A cosmetic composition for external use for improving hair density, comprising at least Moringa peregrina seed oil extract as an active ingredient, a method for preparing said composition, and a cosmetic method for caring for hair

Abstract

The present invention relates to the field of cosmetics, and more specifically to an oil extract of Moringa peregrina seeds as an active ingredient for improving hair density. The present invention also relates to a method for preparing such a composition and a method for cosmetic hair care.

Description

[Technical Field]

[0001] The present invention relates to the field of cosmetics, and more particularly to the field of active ingredients included in topical skin care compositions. The present invention more particularly relates to Moringa peregrina seed oil extract as an active ingredient for improving hair density. The present invention also relates to a method for preparing the composition and a method for cosmetic hair care. [Background technology]

[0002] Those skilled in the art have long known that human hair follicles alternate between the growth (anagen) and shedding (telogen) phases, and that natural human hair loss reflects the balance between these two phases throughout the body. The average ratio of anagen to telogen follicles is approximately 9 (90 / 10). Under normal physiological conditions, natural hair loss is estimated to be several hundred hairs per day. It is also known that certain factors, such as hormonal imbalance, physiological stress, and nutritional malnutrition, can exacerbate the phenomenon of hair loss. Certain scalp skin diseases with inflammatory characteristics, such as psoriasis or seborrheic dermatitis, can significantly exacerbate hair loss. While stopping hair loss is certainly important when attempting to provide an effective solution to the problem of hair loss, it is also necessary to do everything possible to stimulate new hair growth.

[0003] Furthermore, the risks of controversial ingredients in cosmetics are being widely condemned, leading to a decline in the use of chemical treatments and a rise in the demand for natural treatments.

[0004] In view of the above, the applicant has surprisingly discovered that a composition containing an oil extracted from Moringa peregrina seeds as an active ingredient can dramatically improve hair density on the scalp or skin. The oil can be virgin or winterized.

[0005] Virgin oil is obtained solely by physical means such as squeezing or pressing seeds without using any heat or chemical treatment.

[0006] Oil winterization is a process in which oils and other desired oil components are separated from waxes using solvents at low temperatures. To separate the different components, fractional crystallization is used to exploit the differences in melting points, solubility, and volatility of the components.

[0007] Applicant has discovered that the above compositions are particularly effective for regrowing hair and / or slowing the progression of hair loss, and it is believed that the natural composition itself provides improved hair density without the need for added chemical ingredients.

[0008] The Moringaceae family is a single genus (Moringa) and part of the Saharosindian Flora. According to some authors, it comprises 12–14 species and is distributed from East Africa to Asia. The genus is typically divided into three sections, but phylogenetic analysis has not confirmed its monophyletic status. Rather, this analysis reveals clades based on specific morphological characteristics: pachykaul ("bottle trees"), "tuberous trees," and non-bottle trees and non-tuberous trees ("slender trees"). The species Moringa peregrina (Moringa peregrina (Forssk.) Fiori) belongs to the third group. Genetic studies on this genus or family are sparse, but they confirm the existence of this species relative to the other species, particularly the Indian moringa, Moringa oleifera rum. (See especially the articles "Olson, ME 2002, Combining Data from DNA Sequences and Morphology for a Phylogeny of Moringaceae (Brassicales), Systematic Botany 27(1): 55-73; Hassanein, AMA and Al-Soqee, AA, 2018, Morphological and genetic diversity of Moringa oleifera and Moringa peregrina genotypes, Horticulture, Environment and Biotechnology 59(2): 251-261.") A recent article on Moringa peregrina collected from various locations in Saudi Arabia, using ITS markers, concluded that there is genetic stability in this species (Alaklabi, A., 2015, Genetic diversity of Moringa peregrina species in Saudi Arabia with ITS sequences, Saudi Journal of Biological Sciences 22: 186-190), although there is a high level of genetic variation within populations.

[0009] Moringa peregrina is found in rocky environments in Yemen, Oman, Saudi Arabia, East Africa, Sudan, Ethiopia, Eritrea, Somalia, and Djibouti. Its presence in Iran appears to be limited to the southeastern provinces, but this needs confirmation (PROTA14 = Munyanziza E. and Yongabi KA, Vegetable oils / Oleaginous plants, Moringa peregrina (Forssk.) Fiori, http: / / database.prota.org / protahtml / moringa peregrina_fr.htm, accessed October 23, 2019). In the Middle East and Egypt, the species is currently represented only by rare, sparsely distributed relict stations (except for a few high-altitude populations), mainly in the Sudanese sector. Moringa peregrina is considered rare and endangered in Sudan and Yemen today. Compared to other species in its clade, Moringa peregrina inhabits the driest and most hostile habitats. It is significantly more drought-tolerant than Moringa oleifera, which is commercially planted extensively in tropical and subtropical regions. Recent studies have shown that seed size and girth favorably affect germination time and the growth rate and velocity of young individuals (Gomaa NH and Pico FX, 2011, Seed germination, seedling traits, and seed bank of the tree Moringa peregrina (Moringaceae) in a hyper-arid environment, American Journal of Botany 98(6): 1024-1030). This suggests that Moringa peregrina can efficiently reproduce in extreme (hyperarid) abiotic environments by adjusting resource allocation to seed quality over seed number. Moringa peregrina seeds have a thicker central mesocarp than Moringa oleifera seeds in terms of cell layers.

[0010] There are several historical reports that indicate that Moringa peregrina oil was actively traded in the Al-'Ula region at the dawn of Islam (Naseef, AAS, 1995, Al-'Ula, A study of Cultural and Social Heritage). Today, locally produced oil from Moringa peregrina is primarily sold for personal consumption or for the local market. In Saudi Arabia, Moringa peregrina leaves have traditionally been used as an internal decoction to treat diabetes, intestinal disorders, eye disorders, and anemia (Abdel-Kader, MS, Hazazi AMA, Elmakki OA and Alqasoumi SI, 2018, A survey on the traditional plants used in Al Kobah village, Saudi Pharmaceutical Journal 26(6): 817-821), and as a diuretic, rubefacient, and astringent (Aqeel AAM, Tariq M., Mossa JS, Al-Yahya MA and Al-Said MS, 1984, “Plants used in Arabian Folk medicine”, Report submitted to Saudi Arabian National Centre for Science and Technology, Riyadh, Saudi Arabia).In Oman, the oil is extracted by women in late summer and is used to treat migraines, fever, burns, lacerations, fractures, constipation, stomach pains, muscle pain, dry hair, and labor pains (Ghazanfar SA, 1994, Handbook of Arabian Medicinal Plants, 1st ed., CRC Press, Boca Raton, Ann Arbor, US; Ghazanfar SA, 1998, Plants of Economic Importance, cap. 15, in Ghazanfar, SA and Fisher, M. (ed.) Vegetation of the Arabian Peninsula. Geobotany 25, pp. 241-264, Kluwer Academic Publishers, Table 11.1, p. 247 and 11.7, p. 251). It has also been used in fragrance compositions (Ghazanfar SA, 1998, page 259) and as a face lotion in Oman and Yemen (Ghazanfar SA and Rechinger B., 1996, Two multi-purpose seed oils from Oman. Plants for Food and Medicine. Paper presented at the joint meeting of the Society for Economic Botany and International Society for Ethnopharmacology, 1-7 July 1996, London).

[0011] Traditional uses of Moringa peregrina seed oil include treating headaches, fever, constipation, burns, abdominal pain, back and muscle pain, and labor pains (Boulos, L., 2000. Flora of Egypt, vol. I. Al Hadara Publishing, Cairo, p. 238; ElBatran, SA, Abdel-Salam, OM, Abdelshfeek, KA, Nazif, NM, Ismail, SI, Hammouda, FM, 2005. Phytochemical and pharmacological investigation on Moringa peregrina (Forssk) Fiori., Nat. Prod. Sci. 11 (3), 199-206.). Other traditional uses of the seeds include cooking and treating stomach aches (Van der Vossen, HAM, Mkamilo, GS, 2007. PROTA 14: Vegetable Oils. PROTA publishers, Wageningen, pp. 119-120).

[0012] Lalas et al. (Lalas, S., Gortzi, O., Athanasiadis, V., Tsaknis, J., and Chinou, I., 2012, Determination of Antimicrobial Activity and Resistance to Oxidation of Moringa peregrina Seed Oil, Molecules 17, pp. 2330-2334) found that Moringa peregrina seed oil exhibited activity against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Candida albicans, Candida tropicalis, and Candida glabrata. The MICs for each of these microorganisms are 3.5 mg / ml, 3.35 mg / ml, 4.38 mg / ml, 4.8 mg / ml, 4.3 mg / ml, 4.95 mg / ml, 5.7 mg / ml, 3.3 mg / ml, and 3.25 mg / ml, respectively.

[0013] Tsaknis (Tsaknis, J., 1998. Characterization of Moringa peregrina Arabian seed oil. Grasas y Aceites 49 (2), 170-176) studied the chemical composition of Moringa peregrina seed oil and found that palmitic and stearic acids are the main saturated fatty acids, whereas oleic and gadoleic acids are the major unsaturated fatty acids. Among the sterols, β-sitosterol is the most predominant, followed by campesterol, stigmasterol, and brassicasterol. α-, γ-, and δ-tocopherols were also detected.

[0014] A seed fatty acid profiling study by Osman & Abohassan (2012) also revealed that five of the nine fatty acids detected in the seeds were classified as saturated, with the most abundant being oleic acid (53.94%-64.19%), palmitic acid (12.89%-17.88%), and stearic acid (6.63%-11.51%). Another comprehensive analysis of seed oil was conducted by Somali et al. (Somali, MA, Bajneid, MA, Alfhaimani, SS, 1984. Chemical composition and characteristics of M. peregrina seeds and seed oil. Journal of the American Oil Chemists' Society 61 (1), 85-86), which revealed that unsaturated fatty acids accounted for 84.7% of the oil. This unprecedented discovery is summarized by the authors as follows: "Moringa peregrina kernels contain 1.8% water, 54.3% oil, 22.1% protein, 3.6% fiber, 15.3% carbohydrates, and 2.5% ash. The composition and properties of the extracted oil were determined. Gas-liquid chromatography of the fatty acid methyl esters revealed the presence of 14.7% saturated and 84.7% unsaturated fatty acids. The fatty acid composition is as follows: palmitic acid 9.3%, palmitoleic acid 2.4%, stearic acid 3.5%, oleic acid 78.0%, linoleic acid 0.6%, linolenic acid 1.6%, arachidonic acid 1.8%, and behenic acid 2.6%."

[0015] Another study on Iranian Moringa peregrina seed oil (Gharibzahedi, SMT, Ansarifard, I., Hasanabadi, Y., Sadeghi, H., Ghahderijani, M., and Yousefi, R., 2013. Physicochemical properties of Moringa peregrina seeds and its oil. Quality Assurance and Safety Crops and Food 5 (4), 303-309) revealed similar data, with oleic acid (77.9%) being the predominant fatty acid, followed by palmitic acid (9.3%), stearic acid (3.5%), and behenic acid (2.6%). These and other data (e.g., Salaheldeen, M., Aroua, MK, Mariod, AA, Cheng, SF, and Abdelrahman, MA, 2014. An evaluation of Moringa peregrina seeds as a source for biofuel, Industrial Crops and Products 61, 49-61) indicate that polyunsaturated fatty acids are the major components of Moringa peregrina seed oil.

[0016] Persian Moringa peregrina seeds have been studied for their vitamin composition (Asghari, G., Palizban, A., and Bakhshaei, B., 2015. Quantitative analysis of the nutritional components in leaves and seeds of the Persian Moringa peregrina (Forssk.) Fiori, Pharmacognosy Research 7 (3), 242-248). Vitamin C and vitamin A were recorded in sufficient amounts: 14.0 ± 0.6 mg per 100 g dry weight, and 24.8 ± 0.7 mg per 100 g dry weight. Furthermore, elemental analysis of the seeds revealed a calcium content of 1164.8 ± 43.4 mg and a potassium content of 572 mg per 100 g dry weight.

[0017] The oil composition of Moringa peregrina from Saudi Arabia and Egypt, compared with Moringa oleifera, was studied by Robiansyah et al. in 2014 (Robiansyah, I., Hajar, AS, Al-kordy, MA, and Ramadan, A. 2014. Current Status of Economically Important Plant Moringa peregrina (Forsk.) Fiori in Saudi Arabia: A Review, International Journal of Theoretical and Applied Sciences 6(1), 79-86; Table 1, p. 81, and Tables 2 and 3, p. 82). This oil contains high concentrations of tocopherols, consisting of αβ- and δ-tocopherols, at concentrations of 145 mg / kg, 58 mg / kg, and 66 mg / kg, respectively. This high tocopherol content protects the oil during storage and processing. Other studies have found that Moringa peregrina seed oil can withstand temperatures of 120°C for up to 10.5 hours, significantly more than extra virgin olive oil (8.9 hours).

[0018] Cosmetic compositions containing extracts derived from Moringa oleifera seeds are known in the cosmetic arts. For example, U.S. Patent No. 667,047 describes an oxidatively stable emollient made from Moringa oil or its derivatives. The composition described therein has high lubricity and contains Moringa oleifera, a long-chain oil with less than 1% interrupted methylene unsaturation.

[0019] Korean Patent No. 101540333 describes a mixed extract prepared by extracting a mixture of Moringa oleifera, Dictamnus albus, and Annona muricata with purified water. The resulting filtered mixed extract is used to prevent hair loss.

[0020] Korean Patent Publication No. 101947705 describes cosmetic, pharmaceutical, and food compositions for preventing hair loss or promoting hair growth, containing extracts of Mukko and Sappan obtained by ethanol extraction, moringa oil, and black cumin seed oil. The compositions contain 0.01% to 1% by weight, preferably 0.01% by weight, of moringa oil, which is used for its mechanical lubricating activity. The moringa species described in the publication is believed to be Moringa oleifera, based on its geographical origin, as stated in the abstract.

[0021] Finally, Korean Patent Publication No. 102277088 describes a cosmetic composition containing a fermentation product of camellia oil, argan oil, and Moringa oleifera oil as active ingredients used for improving scalp or preventing hair loss, as well as a method for preparing the cosmetic composition.

[0022] All of the above cited prior art compositions are made from mixtures of different extracts, including extracts of Moringa oleifera, for preventing or treating hair loss. None of the above documents describe the use of Moringa oleifera oil alone.

[0023] Therefore, one problem that the present invention proposes to solve is to develop new products based on natural extracts of Moringa peregrina, such as oil of Moringa peregrina, that can be used in cosmetics or pharmaceuticals and that are easy to use to improve hair density or to prevent and treat hair loss.

[0024] The applicant has discovered that a composition containing oil extracted from Moringa peregrina seeds as an active ingredient can dramatically improve hair density on the scalp or skin.

[0025] Applicants have discovered that such novel compositions are particularly effective for regrowing hair and / or slowing the progression of hair loss. Summary of the Invention

[0026] A first aspect of the present invention is a cosmetic topical composition for improving hair density comprising at least Moringa peregrina seed oil extract as an active ingredient, optionally in combination with a cosmetically acceptable excipient (excipient, vehicle, etc.).

[0027] A second aspect of the invention is a method for making the composition of the invention, comprising: a) drying shelled seeds of Moringa peregrina (Forssk.) Fiori, harvested at fruit ripeness, to an internal moisture content of less than 8%, preferably about 6% or less than 6%, more preferably about 4%; b) pressing the dried shelled seeds of step a) by a mechanical endless screw press to separate the dried shelled seed oil from other components of the dried shelled seeds to obtain the dried shelled seed virgin oil and cake; c) then allowing the virgin oil from the dried shelled seeds obtained in step b) to decant, isolate and place in a sterile container, and optionally combine the virgin oil with a cosmetically acceptable excipient; It has.

[0028] A third aspect of the present invention is cosmetic hair care, characterized in that an effective amount of at least about 0.06 g of a composition according to the invention is applied to the scalp and / or hair for at least 5 minutes, preferably 10 minutes, twice daily, and optionally rinsed off with water and / or a conventional shampoo.

[0029] A fourth aspect of the present invention is a dermatological composition for preventing and treating alopecia, comprising at least Moringa peregrina seed oil extract as an active ingredient, and optionally a dermatologically acceptable excipient.

[0030] The present invention will be better understood and other objects, details, features and advantages of the present invention will become more apparent from the following description of several specific embodiments of the present invention, taken in conjunction with the accompanying drawings, in which the specific embodiments described below are merely illustrative and are not intended to limit the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0031] In this specification, when a range is stated, it is inclusive of its upper and lower limits unless otherwise specified.

[0032] For the purposes of the present invention, the following definitions apply:

[0033] - "External composition": the application or spreading of the active principle of the invention or of a composition containing said active principle onto the surface of the skin or scalp or hair.

[0034] - "Cosmetically acceptable" or "dermatologically acceptable": Suitable for external use in contact with human skin without any risk of toxicity, incompatibility, instability or allergic reaction.

[0035] As used herein, the expression "prevention of hair loss" refers to minimizing or preventing hair loss caused by various habits and environmental influences, such as genetic causes, hormonal imbalance, mental stress in social life, exposure to air pollution, consumption of processed foods, etc.

[0036] As used herein, the expression "treatment of hair loss" refers to minimizing the absence of hair and causing hair growth in areas where hair would normally be present but is absent due to various diseases or treatments that cause hair loss.

[0037] - "Shelled seeds": means that the seed shell or pericarp remains attached to the surrounding area of ​​the harvested seed.

[0038] - "When the fruit is ripe": means when the fruit is ripe, preferably when the pod begins to split open, changes color from dark beige to brown, and about the bottom quarter of the pod twists 180°, opening the capsules.

[0039] - "Improves hair density": Stops hair loss and stimulates hair growth, increasing hair density by 1cm 2 This means increasing the density of hair so that the number of hairs per scalp is greater than the number before cosmetic care.

[0040] - "Approximately": The stated information (time, percentage, etc.) has a margin of ±10~20%.

[0041] - "active ingredient" or "active agent": the amount of the extract of the present invention sufficient to achieve the stated biological activity. The proportion of the amount of the active ingredient relative to the total weight of the composition may vary between 5% and 100% by weight depending on the cause of prevention or treatment.

[0042] - "Less than": "Less than" means below that value in the strict sense. When no minimum value is specified, "less than X" means a range between 0 and X excluding X. For example, "less than 8%" means a range between 0% and 8% excluding 8%, "less than 25 μm" means a range between 0 μm and 25 μm excluding 25 μm, and "5% or more and less than 100%" means a range between 5% and 100%, including 5% and excluding 100%.

[0043] A first aspect of the present invention is a cosmetic topical composition for improving hair density comprising an oil extract of Moringa peregrina seeds as an active ingredient, optionally in combination with a cosmetically acceptable excipient.

[0044] Due to the above-mentioned characteristic composition, Moringa peregrina seed extract oil has been found to be particularly effective for improving hair density, for hair regrowth, and / or for slowing the progression of hair loss.

[0045] In embodiments, the composition as described above may have one or more of the following characteristics:

[0046] In one embodiment, the composition comprises virgin oil of Moringa peregrina seeds as the active ingredient.

[0047] In one embodiment, the composition comprises as the active ingredient winterized oil of Moringa peregrina seeds, such winterized oil being as described in Example 1 below.

[0048] In one preferred embodiment, the seeds are shelled seeds of Moringa peregrina (Forssk.) Fiori, which have been harvested at ripe fruit and then dried to an internal moisture content of less than 8%, preferably less than or about 6%, more preferably about 4%. The shelled seeds may be dried to an internal moisture content of less than about 4%.

[0049] Generally, the seeds with shells are dried to an internal moisture content of 2% to 8%.

[0050] In one embodiment, the content of the Moringa peregrina seed oil extract in the composition is 5% to 100% by weight.

[0051] In another embodiment, the content of the Moringa peregrina seed oil extract in the composition is 100% by weight. In this embodiment, the Moringa peregrina seed oil extract does not contain any excipients or other components other than the original extracted components of the seed. In such a case, the content of the Moringa peregrina oil in the composition is 100%. The extracted oil can be virgin oil. In another embodiment, the extracted oil is winterized oil.

[0052] In one embodiment, the composition comprises more than 5% and less than 100% by weight of the Moringa peregrina seed oil extract, as well as other ingredients to improve the formulation, i.e., modify the formulation to make it more suitable for the application.

[0053] Since the composition of the present invention is intended for external application, it optionally comprises a cosmetically acceptable medium, which is a medium compatible with the skin and skin appendages, and covers all cosmetic forms. Such compositions may be in particular creams, oil-in-water emulsions, water-in-oil emulsions, multiple emulsions, serums, solutions, suspensions, gels, milks, lotions, sticks, or powders, and may be suitable for application to the skin and / or skin appendages, such as hair. The composition may also comprise excipients necessary for the formulation, such as solvents, emollients, thickeners, diluents, surfactants, antioxidants, bioactive agents, dyes, preservatives, and fragrances.

[0054] The compositions of the invention may in particular consist of hair care compositions, in particular shampoos, hair conditioners, treatment lotions, styling creams, styling gels, hair restructuring lotions, masks, etc. The cosmetic compositions of the invention may in particular be used in treatments, which may include rinsing after application, or alternatively may be in the form of a shampoo.

[0055] The compositions of the present invention may also contain any additives conventionally used in the envisaged field of application or adjuvants necessary for the formulation, such as solvents, thickeners, diluents, antioxidants, dyes, sunscreens, pigments, fillers, preservatives, fragrances, deodorants, cosmetic or pharmaceutical active ingredients, essential oils, vitamins, refined fatty acids, surfactants, film-forming polymers, etc.

[0056] The ICID (INCI Dictionary & Handbook (“International Nomenclature of Cosmetic Ingredients” (13th ed., 2010), published by The Personal Care Products Council Inc., Washington, D.C., USA) describes a wide variety of cosmetic and pharmaceutical ingredients commonly used in the skin care industry that are suitable for use as additive ingredients in the compositions of the present invention, but this does not limit the present invention.

[0057] In any case, the skilled artisan will take steps to ensure that the adjuvants and their proportions are chosen so as not to adversely affect the desired advantageous properties of the compositions of the invention.

[0058] A second aspect of the invention is a method for making the composition of the invention, comprising: a) drying shelled seeds of Moringa peregrina (Forssk.) Fiori, harvested at fruit ripeness, to an internal moisture content of less than 8%, preferably about 6% or less than 6%, more preferably about 4%; b) pressing the dried shelled seeds of step a) by a mechanical endless screw press to separate the oil contained in the dried shelled seeds from other components of the dried shelled seeds and obtain the dried shelled seed virgin oil and cake; c) thereafter isolating the dried, shelled virgin oil obtained in step b) and placing it in a sterile container; Optionally, the virgin oil is combined with a cosmetically acceptable excipient.

[0059] To obtain the virgin oil of the dried shelled seeds, step b) involves performing a solid-liquid separation, which can be carried out by various processes such as natural decantation, centrifugation and / or filtration.

[0060] In another embodiment, the method for making the composition of the present invention comprises: - carrying out steps a) and b) above to obtain virgin oil of dried shelled seeds of Moringa peregrina; - in step c') subjecting the dried shelled seed virgin oil obtained in step b) to a first crystallization treatment at about 13°C with moderate stirring for 60-180 minutes depending on the batch size, followed by a first vacuum filtration treatment at about 13°C with a filter cut of less than about 25 μm, preferably less than 10 μm, preferably about 5 μm, to obtain the first winterized dried shelled seed oil; - in step d), a second crystallization of the first winterized oil obtained in step c') at about 4°C with moderate stirring for 60 to 180 minutes depending on the batch size, followed by a second vacuum filtration at about 4°C with a filter cut of less than about 25 μm, preferably less than 10 μm, preferably about 5 μm, to obtain a second winterized oil of the dried shelled seeds; - in step e), isolating the second winterized oil obtained in step d) and placing it in a sterile container, and optionally combining the second winterized oil with a cosmetically acceptable excipient; It has.

[0061] Generally, the filtration cutoff of the first vacuum filtration and the second vacuum filtration is between 4 and 30 micrometers (μm).

[0062] A third aspect of the present invention is a method for cosmetic hair care, comprising applying an effective amount of at least about 0.06 g of a composition of the present invention to the scalp and / or hair for at least 5 minutes, preferably 10 minutes, twice daily, optionally followed by rinsing with water and / or a conventional shampoo.

[0063] A fourth aspect of the present invention is a dermatological composition for preventing and treating alopecia, comprising at least Moringa peregrina seed oil extract as an active ingredient, and optionally a dermatologically acceptable excipient.

[0064] In one embodiment, the dermatological composition is further used to prevent and relieve itching.

[0065] The dermatological composition is then used as a medicament for preventing or treating hair loss or for preventing or alleviating itching.

[0066] In one embodiment, the dermatological composition comprises virgin or winterized oil of Moringa peregrina seeds.

[0067] In another preferred embodiment, the seeds are shelled seeds of Moringa peregrina (Forssk.) Fiori harvested when the fruit is dried after ripening to an internal moisture content of less than 8%, preferably about 6% or less, more preferably about 4%, and the shelled seeds are dried to an internal moisture content of 2% to 8%.

[0068] In one embodiment, the content of the Moringa peregrina seed oil extract in the dermatological composition is 5-100% by weight.

[0069] Although the present invention has been described with reference to several specific embodiments, it is clear that this is not a limitation of the present invention, and that the present invention also includes all technical equivalents of the means described herein and all technical equivalents and combinations thereof that fall within the scope of the present invention.

[0070] Use of the verb "comprise" or "include" and its conjugated forms does not exclude the presence of elements or steps other than those stated in a claim.

[0071] In the claims, any reference signs in parentheses shall not be construed as limiting the scope of the claims.

[0072] <Example> Example 1: Preparation of Moringa peregrina seed plant extract

[0073] Shelled seeds of Moringa peregrina (Forssk.) Fiori, harvested at fruit ripeness, are dried to an internal moisture content of less than 8%, preferably less than 6%. The internal moisture content can be about 6%, preferably about 4%. The internal moisture content is 2-8%.

[0074] The dried, shelled seeds were then pressed in a mechanical endless screw press to separate the oil from other components of the seeds, yielding virgin oil and a cake.

[0075] Virgin oil was recovered after solid-liquid separation and / or additional filtration.

[0076] The virgin oil was subjected to a first crystallization process with moderate agitation at about 13°C for 60-180 minutes (depending on the batch size), followed by a first vacuum filtration process at about 13°C with a 4-30 μm filter cut, preferably about 25 μm or less, preferably less than 10 μm, and preferably about 5 μm, to obtain a first winterized oil. The first filtered oil, i.e., the first winterized oil, was subjected to a second crystallization process with moderate agitation at about 4°C for 60-180 minutes (depending on the batch size), followed by a second vacuum filtration process at about 4°C with a 4-30 μm filter cut, preferably about 25 μm or less, preferably less than 10 μm, and preferably about 5 μm, to obtain a second winterized oil. The second winterized oil was then isolated and placed in a sterile container. This is the winterized oil mentioned above. Hereafter, this winterized oil will be referred to as "winterized Alula Peregrina oil."

[0077] Example 2: Measurement of hair density using Aramo SG (trademark) ASG 200F

[0078] The use tests were conducted in a laboratory under the supervision of a dermatologist. The study was designed to: To evaluate the cosmetic effect on the tolerance at the application site as a result of regular application of winterized Alura peregrina oil according to the purpose and specified duration of use; Providing evidence of the activity of winterized Alura peregrina oil by conducting studies to confirm or exclude the properties required for the cosmetic product.

[0079] The evaluation questionnaire format, panel characteristics and number of subjects were in accordance with the requirements. Winterized Alura peregrina oil was applied by the subjects according to the declared usage.

[0080] A declaration was considered valid only if the ratio of positive responses received to the total number of subjects participating in the survey exceeded 50%.

[0081] Description of study subjects in Table 1

[0082] [Table 1] Approved subjects received the winterized Alura peregrina oil to be tested and a specially developed questionnaire, and were required to: Use the product in accordance with the instructions; -No other products with similar effects may be used during the test. - Use the questionnaire received to conduct a detailed evaluation of the product being tested; If any adverse reactions occur to the skin, discontinue use of the product immediately and consult a specialist. The results of the test can be affected by the following factors: - Type and condition of the area where the product is applied, · Individual differences in genetic characteristics, Subject's personal preferences.

[0083] The purpose of this study was to evaluate the direct effect of the winterized Alura peregrina oil of the present invention from Example 1 on hair density. The study was carried out using a special measuring device (ARAMO SG™ ASG 200F) manufactured by ARAM HUVIS. 21 subjects were subjected to an instrumental study. Measurements were taken at 60x zoom in designated zones (before product application (D0), after 56 days (D56) and after 84 days (D84) of regular use). Each subject was required to apply three drops of winterized Alura peregrina oil twice daily, morning and evening (one drop weighing approximately 0.02 g).

[0084] The study was carried out in an air-conditioned room at a temperature of 20±2°C and a relative humidity of 50±10%. Table 2 below shows the mean results of hair density at the product application sites, measured before application (D0), 56 days (D56) and 84 days (D84) after regular use [1 cm 2 [Average hair volume per].

[0085] [Table 2]

[0086] Conclusion: The composition containing winterized Alura peregrina oil (winterized oil) improved hair density by approximately 10% (average) after 56 days of regular use, and by approximately 16% (average) after 84 days of regular use.

[0087] Example 3: Evaluation of the effect of the composition on tolerance at the application site

[0088] Based on the results of medical examinations and interviews collected from the subjects, it was found that the composition tested in Example 1 (winterized Alura peregrina oil) was very well tolerated at the application site. All subjects who completed the study reported no adverse symptoms during regular application, nor any sensations that could indicate an adverse reaction to any of the product's ingredients, such as irritation, burning sensation, redness, or itching. None of the subjects experienced dryness at the application site due to the product.

[0089] Based on the results of the use test, the compositional properties of the winterized Alura peregrina oil were evaluated, and the results are shown in Table 3 below.

[0090] [Table 3]

[0091] Conclusion: The results obtained in the study led to the conclusion that the composition of the present invention, when used as intended, was very well tolerated by those tested who had no contraindications to its use.

[0092] Additionally, the product relieved itching in 70% of the people tested, leading to the conclusion that the composition helps relieve itching.

[0093] Under the conditions of the study, after 84 days of regular application, the following conclusions were made about the composition of winterized Alura peregrina oil: The composition was very well tolerated at the application site. Based on the subject survey, the effectiveness of the declared characteristics was confirmed: -My hair felt softer after application. -The product feels like it's protecting my hair. -After application, my hair became shinier. -The product eliminated bad odors from hair. -Gives hair a neutral or pleasant smell. -My scalp feels clean. -The product relieved the itching. -After application, my hair feels stronger. -After application, my hair seems to get longer. -The product has relieved the itching and I feel like my quality of life has improved. -The product gave my hair volume. -After application, the product did not make my hair feel dry. -The product improved the feel of my hair. -After application, hair feels nourished. There was no change in hair color. -The product restored shine to my hair. -There were no problems with handling or use of the product. -I liked the cosmetic quality of the product.

[0094] Example 4: Comparative Data of Fatty Acid Profiles of Moringa oleifera and Moringa peregrina Seed Oils

[0095] [Table 4]

[0096] Example 5: Hair Styling Agent "Waxy Jelly" Hair Booster: Wet Hair Styling Product

[0097] [Table 5]

[0098] (Example 6: Stimulating Shampoo Hair Booster: Apply a thick layer and leave for 4-10 minutes, then lather and rinse with clean water)

[0099] [Table 6]

[0100] (Example 7: Hair loss cream: Apply to scalp in the morning and evening, massaging in. Brush and style. When applied to long hair, it leaves a wet feeling.)

[0101] [Table 7]

[0102] (Example 8: Stimulating Shampoo Hair Booster: Apply a thick layer to the scalp and leave on for 4-10 minutes, then rinse before washing with shampoo and rinsing with clean water)

[0103] [Table 8]

[0104] (Example 9: Hair Booster Dry Oil: Hair care product. Apply to scalp in the morning and evening, massaging in. Brush through to style. When applied to long hair, it leaves a wet feeling.)

[0105] [Table 9]

[0106] Example 10: Conditioner Hair Booster Powder [Table 10]

[0107] Use after shampooing. Apply to scalp and leave for 5-10 minutes. Sprinkle on wet hair and massage into scalp. Leave in a thick layer for 10 minutes. Rinse with clean water.

Claims

1. A cosmetic composition for external use for improving hair density, comprising at least Moringa peregrina seed extract oil as an active ingredient.

2. The Moringa peregrina seed oil extract is combined with a cosmetically acceptable extract. The composition of claim 1.

3. The extracted oil is virgin oil from Moringa peregrina seeds. The composition according to claim 1 or 2.

4. The extracted oil is winterized oil of Moringa peregrina seeds. The composition according to claim 1 or 2.

5. The seeds are shelled seeds of Moringa peregrina (Forssk.) Fiori, which have been harvested when the fruit is ripe and then dried so that the internal moisture content is less than 8%. The composition according to any one of claims 1 to 4.

6. The shelled seeds are dried so that the internal moisture content is less than 6%. The composition of claim 5.

7. The shelled seeds are dried to an internal moisture content of about 4%. The composition of claim 6.

8. The content of the Moringa peregrina seed extract oil in the composition is 5% by weight to 100% by weight. The composition of any one of claims 1 to 7.

9. The content of the Moringa peregrina seed extract oil in the composition is 100% by weight. The composition of claim 8.

10. The extract of Moringa peregrina seeds contains 5% by weight or more and less than 100% by weight of the oil, and other ingredients for improving the formulation. The composition of claim 8.

11. 4. A method for making the composition of any one of claims 1 to 3, comprising: a) drying shelled seeds of Moringa peregrina (Forssk.) Fiori harvested at fruit ripeness to an internal moisture content of less than 8%; b) pressing the dried, shelled seeds in a mechanical endless screw press to separate the oil from other components of the seeds to obtain virgin oil and a cake; c) thereafter isolating the virgin oil obtained and placing it into a sterile container; A method comprising:

12. In step c), the virgin oil is combined with a cosmetically acceptable excipient. The method of claim 11.

13. 5. A method for making the composition of claim 4, comprising: a) drying shelled seeds of Moringa peregrina (Forssk.) Fiori harvested at fruit ripeness to an internal moisture content of less than 8%; b) pressing the dried, shelled seeds in a mechanical endless screw press to separate the oil from other components of the seeds to obtain virgin oil and a cake; c') subjecting the virgin oil to a first crystallization treatment at about 13°C with moderate stirring for 60-180 minutes depending on the batch size, followed by vacuum filtration at about 13°C with a filter cut of about 25 μm or less to obtain a first winterized oil; d) subjecting the first winterized oil to a second crystallization process with moderate stirring at about 4°C for 60-180 minutes depending on the batch size, followed by vacuum filtration at about 4°C with a filter cutoff of about 25 μm or less to obtain a second winterized oil; e) thereafter isolating the second winterized oil and placing it in a sterile container; A method comprising:

14. In step e), combining the second winterized oil with a cosmetically acceptable excipient.

14. The method of claim 13.

15. A method for cosmetic hair care, comprising:

11. A method comprising applying an effective amount of at least about 0.06 g of a composition according to any one of claims 1 to 10 to the scalp and / or hair for at least 5 minutes twice daily.

16. applying the effective amount to the scalp and / or hair for 10 minutes twice daily; 16. The method of claim 15.

17. Further comprising the step of rinsing with water and / or a regular shampoo.

17. The method according to claim 15 or 16.

18. A dermatological composition for preventing and treating alopecia, comprising at least Moringa peregrina seed oil extract as an active ingredient.

19. including dermatologically acceptable excipients, 19. The dermatological composition of claim 18.

20. 20. Dermatological composition according to claim 18 or 19 for preventing and relieving itching.

21. The extracted oil is virgin or winterized oil of Moringa peregrina seeds. Dermatological composition according to any one of claims 18 to 20.

22. The seeds are shelled seeds of Moringa peregrina (Forssk.) Fiori harvested by drying the fruit after ripening so that the internal moisture content is less than 8%. Dermatological composition according to any one of claims 18 to 21.

23. The content of the Moringa peregrina seed extract oil in the dermatological composition is 5 to 100% by weight.

22. The dermatological composition of claim 21.

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