Laser light adapter, multiphoton microscope body and optical system

Abstract

The present disclosure provides a laser light adapter, a multi-photon microscope system, and an optical system. The laser light adapter includes a case, and an optical beam transformer and an optical beam stabilizing device provided within the case. The case has a laser light input port and a laser light output port. The optical beam transformer is configured to transform a laser light beam entering the case. The optical beam stabilizing device is provided downstream of the optical beam transformer in the laser light transmission direction and is used to adjust the laser light transmission direction to correct a deviation between the actual position of the laser light beam at the laser light output port and its ideal position. According to the technical solution provided by the present disclosure, when laser light with different parameters is input or the laser light is deflected during transmission, the laser light adapter can convert the input laser light or adjust the laser light transmission direction, without the need to readjust the optical path or replace optical elements in the optical path, so that the laser light can be adaptively coupled to a subsequently connected device.

Description

[Technical Field]

[0001] The present disclosure relates to the field of optics, and more particularly to a laser light adapter, a multiphoton microscope body, and an optical system. [Background technology]

[0002] The conventional laser beam coupling method usually uses an adjustment mechanism with multiple degrees of freedom, and the adjustment mechanism is equipped with a lens, and by adjusting the lens, the spatial laser beam is coupled and enters the optical fiber. In order to ensure the coupling efficiency of the laser beam, it is usually necessary to select the lens according to the laser device output light spot size, beam waist position, etc.

[0003] However, when the laser device is replaced or the light spot size is changed, the optical path needs to be readjusted and the lens needs to be reselected, but the optical path adjustment and lens replacement are complicated and require the operation by a specialist.

[0004] Furthermore, if the environment changes, for example, vibrations or temperature changes, the output beam angle of the laser device also changes. A typical laser device has an output beam angle of 25 μrad / °C, and a 10°C change in temperature will cause a 250 μrad change in beam angle. When the laser beam angle changes, the coupling efficiency of the optical fiber drops sharply, significantly affecting the performance of the device. Furthermore, if the beam is deflected by human error, the system must be re-debugged. Summary of the Invention [Problem to be solved by the invention]

[0005] In view of this, the present disclosure provides a laser light adapter, a multiphoton microscope body, and an optical system, which solves problems with laser coupling means in conventional technology, such as complex optical path adjustment and lens replacement, and reduced laser light coupling efficiency due to the laser light being susceptible to change. [Means for solving the problem]

[0006] According to one aspect of the present disclosure, there is provided a laser light adapter, the laser light adapter including a case, and a light beam transformer and a light beam stabilization device provided within the case, the case having a laser light input port and a laser light output port, the light beam transformer is configured to transform a laser light beam entering the case, and the light beam stabilization device is provided downstream of the light beam transformer in a laser light transmission direction and is configured to adjust the laser light transmission direction to correct a deviation between an actual position and an ideal position of the laser light beam at the laser light output port.

[0007] According to another aspect of the present disclosure, the present disclosure provides a multiphoton microscope body to solve problems of prior art multiphoton microscopes, such as large space occupied, difficulty in transportation and movement, and complex installation and maintenance. The multiphoton microscope body according to the present disclosure is configured to be connected to a microscope probe, and the multiphoton microscope body includes a mounting body and a wide-field search module, a laser light coupling module, a fluorescence collection module, and a scanning control module integrated in the mounting body, the wide-field search module is configured to perform wide-field imaging on a living body to search for a target area in the living body for attaching a microscope probe, the laser light coupling module is configured to receive laser light and adjust the laser light to couple the laser light into a laser light transmission optical fiber, the laser light transmission optical fiber is configured to connect the laser light coupling module and the microscope probe, the scanning control module is connected to the microscope probe via a control cable and configured to control the microscope probe to perform laser scanning and generate a fluorescence signal, and the fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection optical fiber and configured to collect the fluorescence signal output by the microscope probe.

[0008] According to another aspect of the present disclosure, the present disclosure further provides an optical imaging system that solves problems such as the need to readjust the optical path when the laser light emitted by the laser device changes, resulting in poor flexibility in use in conventional optical imaging systems. The optical system according to the present disclosure includes a laser device, a transmission optical fiber, an application device main body, and the above-mentioned laser light adapter, wherein the laser device is installed at a laser light input port of the laser light adapter and configured to emit laser light into the laser light input port, a laser light coupler is connected to one end of the transmission optical fiber, the laser light coupler is connected to the laser light output port, and the other end of the transmission optical fiber is connected to the application device main body.

[0009] According to yet another aspect of the present disclosure, the present disclosure further provides an optical system including a laser device, a laser light adapter, and a microscope body, wherein the laser device is configured to emit laser light to the laser light adapter, the laser light adapter is configured to receive the laser light emitted by the laser device, adjust the laser light, and then transmit the adjusted laser light to the microscope body, and the microscope body is configured to transmit the laser light to a microscope probe and control the microscope probe to laser scan a living body to generate a fluorescent signal for imaging. [Effects of the Invention]

[0010] According to the technical solution of the present disclosure, a laser light adapter is provided with an optical beam converter and an optical beam stabilizer. The optical beam converter performs beam conversion on the input laser light, matching the laser light with a downstream device and optimizing the performance of the device. The optical beam stabilizer adjusts the deflection direction of the laser light when a deviation in the laser light beam is detected, ensuring the stability of the laser light output and ensuring the coupling efficiency of the laser light output. Therefore, according to the laser light adapter of the present disclosure, when laser light with different parameters is input or the laser light is deflected during transmission, there is no need to readjust the optical path or replace optical elements in the optical path. Simply converting the input laser light or adjusting the laser light transmission direction allows the laser light to be adaptively coupled to a downstream device.

[0011] The multiphoton microscope integrates each functional module into a single integrated structure, significantly reducing the space required and making it suitable for a variety of laboratories. The integrated structure also allows for neat and tidy wiring. The multiphoton microscope is small in size, portable, and easy to transport and move. The position and orientation of the multiphoton microscope can be quickly adjusted to meet various testing needs, making it suitable for a wider range of applications. The multiphoton microscope also facilitates quick installation and maintenance on-site.

[0012] In the optical system according to the present disclosure, laser light from a laser device passes through a laser light adapter, which performs conversions such as magnification, reduction, and focal length conversion on the laser light beam, converts various different received laser light signals into a unified laser signal output, and outputs the laser light so that the laser light can be adapted to a device connected thereto later, which is advantageous for optimizing system performance. This allows the use of various laser devices with different parameters, and even if the distance from the laser device changes, the received laser light can be converted by the laser light adapter and then output to the microscope body as an adapted laser light beam.

[0013] Other features and advantages of the present disclosure are described in detail in the detailed description section that follows. [Brief explanation of the drawings]

[0014] The drawings that constitute a part of this disclosure are used to provide a further understanding of the disclosure. The exemplary embodiments and descriptions of the disclosure are used to explain the disclosure and are not intended to unduly limit the disclosure. [Figure 1] 1 is a schematic diagram illustrating an appearance of a laser light adapter according to an embodiment of the present disclosure. [Figure 2] 1 is a structural schematic diagram of a laser light adapter with a top cover removed according to an embodiment of the present disclosure. FIG. [Figure 3] FIG. 1 is a structural schematic diagram of an optical system according to an embodiment of the present disclosure. [Figure 4] FIG. 1 is a schematic diagram of an optical path of an optical system according to an embodiment of the present disclosure. [Figure 5] 1 is a schematic diagram illustrating the external configuration of a multiphoton microscope main body according to an embodiment of the present disclosure. [Figure 6] FIG. 6 is a structural schematic diagram of the multiphoton microscope body shown in FIG. 5 with the light-shielding door in an open state. [Figure 7] 1 is a structural schematic diagram of a multiphoton microscope main body in an exploded state according to an embodiment of the present disclosure; [Figure 8] FIG. 10 is a structural schematic diagram of the multiphoton microscope main body in an exploded state, seen from another angle. [Figure 9] FIG. 10 is a schematic diagram illustrating the mating and attachment of a movement module, a living body attachment device, and a visual field search adapter according to one embodiment of the present disclosure. [Figure 10] 1 is a structural schematic diagram of a living body attachment device according to an embodiment of the present disclosure. [Figure 11] 1 is a structural schematic diagram of a laser beam coupling module according to an embodiment of the present disclosure. [Figure 12] 12 is a schematic diagram of the internal structure of the laser beam coupling module shown in FIG. [Figure 13]FIG. 1 is a structural schematic diagram of a wide-field search module attached to a laser beam coupling module according to an embodiment of the present disclosure. [Figure 14] FIG. 2 is a structural schematic diagram of a control box according to an embodiment of the present disclosure. [Figure 15] FIG. 15 is a front view of the control box shown in FIG. 14. [Figure 16] 1 is a structural schematic diagram of a storage device according to an embodiment of the present disclosure. [Figure 17] 1 is a structural schematic diagram of a storage device according to an embodiment of the present disclosure in a disassembled state; [Figure 18] FIG. 1 is a structural schematic diagram of an optical system according to an embodiment of the present disclosure. [Figure 19] FIG. 10 is a structural schematic diagram of an optical system according to another embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0015] Hereinafter, the technical solutions in the embodiments of the present disclosure will be clearly and completely described with reference to the drawings in the embodiments of the present disclosure. Needless to say, the described embodiments are only some of the embodiments of the present disclosure, and are not all of the embodiments. Based on the embodiments of the present disclosure, those skilled in the art can obtain all other embodiments without creative efforts, all of which fall within the scope of protection of the present disclosure. Unless contradictory, the embodiments and features of the embodiments in the present disclosure can be combined with each other.

[0016] In the description of this disclosure, the orientations or positional relationships indicated by terms such as "center," "longitudinal," "lateral," "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "axial," "radial," and "circumferential" are based on the orientations or positional relationships shown in the drawings, and are intended merely to make the disclosure easier to explain and simplify the description, and do not indicate or imply that such devices or parts must have a specific orientation or be constructed or operated in a specific orientation, and therefore should not be understood as limitations on the disclosure. Furthermore, "inside" and "outside" refer to the inside and outside of the contours of each member itself.

[0017] Additionally, the terms "first" and "second" are for descriptive purposes only and should not be understood as indicating or implying relative importance or the number of such features. Thus, features qualified by "first" or "second" may explicitly or implicitly include at least one such feature.

[0018] The present disclosure provides a laser light adapter, and as shown in FIGS. 1 and 2 , the laser light adapter 300 includes a case 3001, and a light beam converter 304 and a light beam stabilizer provided in the case 3001. The case 3001 has a laser light input port 3011 and a laser light output port 3012. The light beam converter 304 is configured to convert a laser light beam entering the case 3001. The light beam stabilizer is provided downstream of the light beam converter 304 in the laser light transmission direction, and is configured to adjust the deflection direction of the laser light beam to correct a deviation between the actual position of the laser light beam at the laser light output port 3012 and an ideal position.

[0019] The laser light adapter according to the present disclosure can perform light beam conversion on the input laser light using the light beam conversion device 304. Light beam conversion means performing expansion, contraction, focal length conversion, etc. on the light beam using the conversion characteristics of the optical element to match the laser light with the subsequent device and optimize the performance of the equipment. In addition, the laser light adapter according to the present disclosure can adjust the deflection direction of the laser light when a deviation in the laser light beam is detected, stabilize the laser light output, and ensure the coupling efficiency of the laser light output.

[0020] Specifically, when laser devices with different parameters are used, and the laser devices emit laser beams with different parameters into the laser beam adapter, the light beam can be converted by the light beam conversion device to unify and output light beams with a consistent light spot size, so that the laser beams output from different laser devices are all compatible with the equipment connected later; or, even if the distance of the laser devices changes, a unified laser beam can be output after the focal length conversion process is performed by the light beam conversion device 304.

[0021] When the environment changes (for example, changes in temperature or humidity, or vibration occurs), each element in the optical path (for example, the laser device itself, reflecting mirror, dichroic mirror, etc.) is subjected to vibration or shifts due to the influence of temperature, causing the output direction of the laser light to change. The light beam stabilization device can adjust the deflection direction of the laser light in real time according to the deviation between the actual position of the light beam at the laser light output port 3012 and the ideal position, ensuring stable output of the laser light, i.e., controlling the laser light to be output within a range with small deviation from the ideal output position, thereby guaranteeing the coupling efficiency of the laser light output.

[0022] Therefore, according to the laser light adapter of the present disclosure, when laser light with different parameters is input or when deflection occurs during laser light transmission, it is not necessary to readjust the optical path or replace optical components in the optical path; instead, the laser light adapter simply converts the input laser light or adjusts the laser light transmission direction, allowing the laser light to be adapted and coupled to the device connected later.

[0023] In one embodiment, the light beam transforming device 304 can adopt a conventional device that can expand or reduce the cross section of the laser light beam and perform focal length transformation on the laser light beam, and its specific structure can be realized by those skilled in the art, so the description will be omitted here.

[0024] The light beam stabilizer is located downstream of the light beam transformer 304 in the laser light transmission direction, so that the light beam stabilizer can correct light beam deflection caused by adjustments such as light beam expansion, contraction, or focal length conversion made by the light beam transformer 304 to the laser light beam.

[0025] As shown in Fig. 2, the light beam stabilization device includes a position detector 307, at least one deflection reflector, and a reflector adjustment mechanism connected to each deflection reflector. The position detector 307 is provided in a position close to the laser light output port 3012 and is configured to detect position information of the laser light at the laser light output port 3012. Specifically, a dichroic mirror 309 is provided in the laser light transmission path, and the dichroic mirror 309 reflects a portion of the laser light directly or indirectly to the position detector 307, thereby realizing detection of the laser light position by the position detector 307. 2 In the embodiment shown in FIG. 1, the dichroic mirror 309 reflects a portion of the laser light to the laser light power meter 308, which is also provided with a dichroic mirror, and the laser light power meter reflects a portion of the laser light to the position detector 307. The position detector 307 may be a 4D position detector, which can precisely detect the position drift and angle drift of the light beam and accurately detect the real-time position of the light beam.

[0026] The reflecting mirror adjustment mechanism is configured to be able to adjust the transmission direction of the laser beam by deflecting and driving the deflecting reflecting mirror based on position information detected by the position detector 307 in order to adjust the transmission direction of the laser beam.

[0027] Specifically, first, an ideal position of the laser light beam at the laser light output port 3012 is determined, and the ideal position is a position where ideal coupling efficiency can be achieved when the laser light is output and coupled to a device or member (e.g., a transmission optical fiber) connected to the laser light output port 3012. When the light beam is deflected, for example, when an optical component is displaced due to vibration or temperature change, or when the light beam is deflected by an artificial touch, the position detector 307 detects the position information of the laser light at the laser light output port 3012 in real time and transmits it to the control unit. The control unit continuously determines the deviation between the position of the laser light beam and the ideal position based on the position information, and further controls the reflecting mirror adjusting mechanism to adjust the deflecting reflecting mirror and constantly adjust the reflection direction of the laser light, so that the laser light is stably transmitted within a certain range surrounding the ideal position.

[0028] The laser light adapter further includes a control unit, which receives position information detected by the position detector 307 and controls the reflector adjusting mechanism based on the position information. In some embodiments, the control unit may be provided within the case 3001, but of course, the control unit may be provided independently, i.e., the control unit may be a single module provided outside the case 3001.

[0029] As shown in FIG. 2, a drive circuit 3016 is further provided inside the case 3001 of the laser light adapter, and the control unit transmits a control signal to the drive circuit 3016, which controls the operation of the reflecting mirror adjustment mechanism.

[0030] In one embodiment, the laser light adapter 300 further includes at least one fixed reflecting mirror configured to change the laser light transmission direction, and the fixed reflecting mirror is provided upstream of the light beam transforming device 304 in the laser light transmission direction. By providing a fixed reflecting mirror to change the laser light transmission direction, the light path can be bent, which facilitates the arrangement of each component in the light path and is advantageous for reducing the overall volume of the laser light adapter.

[0031] In the embodiment shown in FIG. 2, the at least one fixed mirror includes a first fixed mirror 302 and a second fixed mirror 303, and the at least one deflecting mirror includes a first deflecting mirror 305 and a second deflecting mirror 306.

[0032] The laser light is reflected by the first fixed reflecting mirror 302 to the second fixed reflecting mirror 303, which reflects the laser light to the light beam converting device 304, and after the light beam converting device 304 converts the laser light, the output laser light is reflected by the first deflecting reflecting mirror 305 to the second deflecting reflecting mirror 306, which is configured to reflect the laser light to the laser light output port 3012.

[0033] More specifically, the incident angle and exit angle of the laser light at the first fixed reflecting mirror 302, and the incident angle and exit angle at the second fixed reflecting mirror 303 are each approximately 45°, and the incident angle and exit angle of the laser light at the first deflecting reflecting mirror 305, and the incident angle and exit angle at the second deflecting reflecting mirror 303 are each approximately 45°.

[0034] The transmission path of the laser light is shown in Fig. 3. As shown in Fig. 3, the laser light emitted from the laser device 200 enters from a laser light input port 3011, is transmitted to a first fixed reflecting mirror 302, is deflected by the first fixed reflecting mirror 302 by about 90°, is reflected by a second fixed reflecting mirror 303, is further deflected by the second fixed reflecting mirror 303 by about 90°, is transmitted to a light beam converting device 304, is converted into a light beam by the light beam converting device 304, is transmitted to a first deflecting reflecting mirror 305, is deflected by the first deflecting reflecting mirror 305 by about 90°, is reflected by a second deflecting reflecting mirror 306, is then reflected by the second deflecting reflecting mirror 306 to a laser light output port 3012, and is coupled to a component connected to the laser light output port 3012, such as a transmission optical fiber 301 connected to the laser light output port 3012 via a laser light coupler 3014.

[0035] In this embodiment, the optical path is bent by the fixed reflecting mirror and the deflecting reflecting mirror, thereby reducing the length of the laser light adapter and facilitating the arrangement of each optical component.

[0036] The arrangement of the fixed reflecting mirrors and the deflecting reflecting mirrors is not limited to the above description, and other arrangements are also possible.

[0037] In one embodiment, at least one laser light power meter 308 is provided in the case 3001 and configured to detect the power of the laser light entering the laser light adapter. The laser light power meter 308 can detect the change in the power of the laser light in real time to determine whether there is a problem in the laser light transmission, particularly whether there is a problem at the input end of the laser light, such as whether the laser device is damaged or whether the laser light is blocked.

[0038] Optionally, the laser light power meter 308 can be located near the laser light output port 3012 to detect the power of the laser light as it leaves the laser light adapter.

[0039] Specifically, as shown in FIG. 3, a dichroic mirror 309 is provided in the laser light transmission path, and the dichroic mirror 309 can reflect some component light of the laser light beam to a laser light power meter 308, which can obtain the power of the laser light by detecting the component light.

[0040] Alternatively, the at least one laser power meter may include a first laser power meter and a second laser power meter, with the first laser power meter located near the laser input port 3011 and the second laser power meter located near the laser output port 3012. That is, by detecting the power of the laser input using the first laser power meter and the power of the laser output using the second laser power meter, and detecting a change in the power of the laser input, it is possible to determine whether a problem has occurred with the laser input, such as whether the laser device is damaged or blocked. By detecting the power of the laser input and output using the first laser power meter and the second laser power meter, respectively, it is possible to compare the change in the power of the laser output with that of the laser input, thereby determining the power loss of the laser in the laser adapter.

[0041] In one embodiment, the laser light adapter 300 further includes a switch device 3010 provided at the laser light input port 3011, and the switch device 3010 includes an opening / closing door used to open and close the laser light input port 3011, and a door drive mechanism that drives the opening / closing door to convert between an open state and a closed state.

[0042] When the door is opened, the laser light enters the laser light adapter 300 and is transmitted, and when the door is closed, the laser light is prevented from entering the laser light adapter.

[0043] The door drive mechanism may be controlled by a control unit, which sends a control signal, and the drive circuit 3016 can control the door drive mechanism to drive the opening and closing of the door.

[0044] In addition, a support leg 3013 may be further provided below the case 3001 of the laser light adapter 300, and the support leg 3013 is configured to be adjustable in height. By adjusting the height of the support leg 3013, the laser light input port 3011 can be adapted to the height of the laser device, so that the laser device can accurately emit laser light to the laser light input port 3011.

[0045] The height of the support leg 3013 can be adjusted by using conventional techniques, for example, by providing an adjustment bolt to adjust the height of the support leg 3013, or by the support leg including two parts that are connected at different height positions to adjust the height.

[0046] Another embodiment of the present disclosure further provides an optical system. Direct recording of neuronal activity in freely moving living animals is one of the most direct and effective methods for studying the relationship between animal behavior and neural function. Furthermore, multiphoton optical imaging systems, with their excellent optical sectioning capabilities and deep penetration depth, are the most important and widely used tools for neuronal observation. The multiphoton optical imaging system may be a nonlinear laser scanning microscope, such as a two-photon, three-photon, or Raman microscope.

[0047] In conventional multi-photon optical imaging systems, the laser device and optical adjustment bracket are fixed to the optical stage to adjust the optical path, and after the optical path is shaped, it enters the microscope body through a reflecting mirror.Since the optical path from the laser device to the microscope body is a spatial optical path, the microscope body must be stably fixed to the optical stage to ensure that the optical path inside the body is not deflected by external forces and affects the performance of the microscope.

[0048] However, in the periphery, there are usually optical beam shaping ModuleMany modules are installed, such as a circuit control module, various drivers, a fluorescence collection module, a wide-field fluorescence module, and a laser module, making the equipment and wiring complex. In addition, the optical path is easily deflected due to signal interference and human error between each module.

[0049] In addition, the optical path and the microscope body are fixed, and experiments that require the position and direction of the microscope body cannot be flexibly adapted or implemented, for example, if the laser optical path and the microscope body are not on the same stage or even in the same room, they cannot be implemented in the conventional manner. When the laser device is replaced or the distance between the laser devices changes, the laser light emitted by the laser device will change, and in either case, all the optical paths must be readjusted, and further, there may be large differences in the parameters of the laser devices, making it impossible to adapt.

[0050] The present disclosure provides an optical system, which includes a laser device 200, a transmission optical fiber 301, an application device main body 100, and the laser light adapter 300 according to the above embodiment, as shown in Fig. 3, where the application device main body 100 may be a laser-operated device and a microscope main body, such as a two-photon microscope. The laser device 200 is configured to emit laser light to the laser light adapter 300, which receives the laser light emitted from the laser device 200, adjusts and adapts the laser light, and then transmits the adjusted laser light to the application device main body 100, which is configured to transmit the laser light to a microscope probe and control the microscope probe to perform laser scanning on a living body to generate a fluorescent signal for imaging.

[0051] In the technical solution disclosed herein, the laser light from the laser device 200 passes through the laser light adapter 300, which performs conversions such as magnification, reduction, and focal length conversion on the laser light beam, converts various different received laser signals into a unified laser signal and outputs the unified laser signal, so that the laser light can be adapted to the connected device later, which is advantageous for optimizing system performance. This allows various laser devices with different parameters to be used, and even if the distance from the laser device changes, the received laser light can be converted by the laser light adapter 300 and then output an adapted laser light beam to the application device main body 100.

[0052] As shown in FIG. 4, the optical system may further include a microscope probe 400, and the application device body 100 may be a multi-photon microscope body.

[0053] The laser device 200 is provided at the laser light input port 3011 of the laser light adapter 300, and is configured to emit laser light to the laser light input port 3011. A laser light coupler 3014 is connected to one end of the transmission optical fiber 301, and the laser light coupler 3014 is connected to a laser light output port 3012 and the other end of the transmission optical fiber 301 is connected to the application device main body 100.

[0054] The laser light adapter 300 stably couples the laser light into the transmission optical fiber 301, and then transmits it to the application device main body 100 through the transmission optical fiber 301. It can also adjust various laser lights with different parameters emitted from the laser device 200 to suit the application device main body, ensuring that the application device main body is not affected by changes in the parameters of the laser device 200 or changes in the distance between the laser device 200 and the laser light adapter 300. This makes it easy to use, without the need to replace optical components in the optical path or adjust the entire optical path.

[0055] Specifically, the laser device 200 is configured to emit laser light to the laser light adapter 300, the laser light adapter 300 receives the laser light emitted from the laser device 200, adjusts and adapts the laser light, and then transmits the adjusted and adapted laser light to the application device main body 100, the application device main body 100 transmits the laser light to the microscope probe 400, and controls the microscope probe 400 to laser scan the living body and generate a fluorescent signal for imaging.

[0056] In the technical solution disclosed herein, the laser light from the laser device 200 passes through the laser light adapter 300, which performs conversions such as magnification, reduction, and focal length conversion on the laser light beam, converts various different received laser signals into a unified laser signal and outputs it, so that the laser light can be adapted to the subsequently connected device, which is advantageous for optimizing system performance. This allows various laser devices with different parameters to be used, and even if the distance to the laser device changes, the received laser light can be converted by the laser light adapter 300 and then output an adapted laser light beam to the application device main body 100.

[0057] The optical system according to the present disclosure is a multi-photon imaging system, i.e., the microscope probe 400 may employ nonlinear laser scanning imaging such as two-photon, three-photon, Raman, etc. In some embodiments, the microscope probe 400 may specifically include a MEMS (Micro-Electro-Mechanical System) scanning galvanometer mirror and various lenses.

[0058] In one embodiment, the optical system further includes a transmission optical fiber 301 connected between the laser light adapter 300 and the application device main body 100, and the laser light adapter 300 transmits the conditioned laser light from the transmission optical fiber 301 to the application device main body 100. One end of the transmission optical fiber 301 may be connected to a laser light coupler and connected to an output end of the laser light adapter 300 via the laser light coupler, and the other end may be connected to a collimator and connected to the application device main body 100 via the collimator.

[0059] The laser light adapter 300 and the application device main body 100 are connected by an optical fiber, allowing the application device main body 100 to move freely, which allows the application device main body 100 to be placed in different positions as needed, and even placed across a stage, making it more flexible to use.

[0060] Furthermore, the output from the optical fiber can shape the light beam, making the light spot output from the laser light adapter 300 to the application device main body 100 more uniform, which is advantageous for improving system performance. Furthermore, compared to a mode in which a fixed light path adjustment device is provided between the laser light adapter 300 and the application device main body 100, the connection using the optical fiber reduces interference and operational errors, improves system stability, and reduces the need for light path adjustment devices in front of the module, making installation and maintenance easier.

[0061] In one embodiment, the application device body 100 includes a laser light coupling module 3, the laser light input end 31 of the laser light coupling module 3 is connected to a transmission optical fiber 301, and the laser light output end 32 is connected to a microscope probe 400 via a laser light transmission optical fiber 401.

[0062] The laser beam coupling module 3 conditions the laser beam received from the transmission optical fiber 301 and then transmits the conditional laser beam to the microscope probe 400 via the laser beam transmission optical fiber 401. For example, the laser beam coupling module 3 may perform dispersion compensation and / or intensity adjustment on the laser beam.

[0063] 4 is a schematic diagram of an optical path of an optical system according to an embodiment of the present disclosure. As shown in FIG. 4, the laser light adapter 300 includes a laser light power meter 308 for detecting the laser light power, and the laser light coupling module 3 includes a power detector 311 configured to detect the laser light power.

[0064] The laser light power meter 308 can detect the power of the laser light that has entered the laser light adapter 300 in real time, and can detect whether an abnormality has occurred in the laser light transmission based on the power change detected by the laser light power meter 308. This is usually used to determine whether the laser device 200 is damaged or whether the laser light has been blocked. Specifically, Da A chromatic mirror 309 is provided, and a part of the component light of the laser light beam is Da The laser beam is split by the chromatic mirror 309 into a laser beam power meter 308, which detects the component beams to obtain the power of the laser beam. The power detector 311 can detect the power of the laser beam in real time, and similarly, by acquiring the component beams of the laser beam, the power detector 311 detects the component beams to obtain the laser beam power. By comparing the power changes of the power detector 311 and the laser beam power meter 308, it can be determined whether or not there is an abnormality in the laser beam transmission between the laser beam adapter 300 and the application device main body 100. Therefore, by providing the laser beam power meter 308 and the power detector 311, it is possible to quickly locate a part that has failed during laser beam transmission.

[0065] Optionally, a laser light power meter 308 measures the laser light output of the laser light adapter 300. mouth 3012, and a power detector 311 is provided near the laser light input end 31 of the laser light coupling module 3. If the power change of the power detector 311 is larger than that of the laser light power meter 308, it can determine that the laser light power meter 308 has failed, so that the failed part can be quickly located and repaired.

[0066] The transmission path of the laser light will be described below with reference to FIG.

[0067] The laser light emitted from the laser device 200 is mouthThe light enters from 3011, is transmitted to the first fixed reflecting mirror 302, is deflected by about 90° by the first fixed reflecting mirror 302, is reflected by the second fixed reflecting mirror 303, is further deflected by about 90° by the second fixed reflecting mirror 303, is transmitted to the light beam converting device 304, is converted into a light beam by the light beam converting device 304, is transmitted to the first deflecting reflecting mirror 305, is further deflected by about 90° by the first deflecting reflecting mirror 305, is reflected by the second deflecting reflecting mirror 306, and is output as a laser light output mouth 3012, and the laser light output mouth 3012 is connected to the transmission optical fiber 301.

[0068] The light beam transmitted by the transmission optical fiber 301 enters the laser light coupling module 3 from the laser light input end 31, is dispersion compensated by the dispersion compensation element 34, is transmitted to the reflecting mirror 35, is deflected by about 90 degrees by the reflecting mirror 35, and is transmitted to the acousto-optic modulator 36. The laser light is intensity-adjusted by the acousto-optic modulator 36, and is then 3 The beam is transmitted to the deflection mirror 37, 3 After being deflected by about 90 degrees by the deflection mirror 37, 4 The light is reflected by the deflection mirror 38. 4 The laser beam is reflected by the deflecting reflector 38 to the laser beam output end 32 and coupled to the laser beam transmission optical fiber 401 connected to the laser beam output end 32 .

[0069] The optical system according to the present disclosure further includes a workbench, which includes a workbench body and a display, and the workbench body is connected to the application device body 100, which processes the collected fluorescent signal and transmits it to the workbench body, and the display displays the image, and the workbench body further sends control commands to the application device body 100, and then each control circuit in the control box 5 controls each component.

[0070] The optical system according to the present disclosure may further include a behavioral testing device that provides an activity space for a living organism to which the microscope probe 400 is attached. For example, a mouse to which the microscope probe 400 is attached is placed in the behavioral testing device and allowed to move freely, and the state of neurons when the mouse is in a freely moving state can be detected.

[0071] Meanwhile, one embodiment of the present disclosure provides an application device. Direct recording of neuronal activity in freely moving animals is one of the most direct and effective methods for studying the relationship between animal behavior and neural function, and multiphoton microscopes are the most important and most widely used tools for observing animal neurons through fluorescence imaging. Multiphoton microscopes can be nonlinear laser scanning microscopes such as two-photon, three-photon, and Raman microscopes.

[0072] Currently, multi-photon microscope devices have problems such as a complex structure and large volume, occupying a large space, requiring many connecting wires, making transportation and movement difficult, and making on-site installation and subsequent maintenance complicated.

[0073] The application device main body according to one embodiment of the present disclosure is a multiphoton microscope main body 100 configured to connect to a microscope probe, which is detachably attached to a living organism to observe the neuronal activity of the living organism.

[0074] As shown in Figures 5 to 8, a multiphoton microscope body 100 according to an embodiment of the present disclosure includes a mounting body 1, a wide-field search module 4, a laser light coupling module 3, a fluorescence collection module, and a scanning control module integrated in the mounting body 1, wherein the wide-field search module 4 is configured to perform wide-field imaging on a living body to search for a target area in the living body for attaching a microscope probe, the laser light coupling module 3 is configured to receive laser light and adjust the laser light to couple the laser light to a laser light transmission optical fiber, which is configured to connect the laser light coupling module 3 and the microscope probe, the scanning control module is connected to the microscope probe via a control cable and configured to control the microscope probe to laser scan the living body and generate a fluorescence signal, and the fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection optical fiber and configured to collect the fluorescence signal output by the microscope probe.

[0075] In application, the multiphoton microscope main body 100 is connected to a microscope probe 400. Specifically, as shown in Figures 18 and 19, the laser light coupling module 3 of the multiphoton microscope main body 100 is connected to the microscope probe 400 via a laser light transmission optical fiber 401, the scanning control module is connected to the microscope probe 400 via a control cable 403, and the fluorescence collecting module is connected to the microscope probe 400 via a fluorescence collecting optical fiber 402, and the microscope probe 400 is configured to be attached to a living body. When the laser light coupling module 3 receives and transmits laser light to the microscope probe 400 via the laser light transmission optical fiber 401, the scanning control module controls the microscope probe 400 via the control cable 403 to laser scan the living body and generate a fluorescence signal. The fluorescence collecting module collects the fluorescence signal output by the microscope probe 400 via the fluorescence collecting optical fiber 402, converts the output fluorescence signal into an electrical signal, and sends it to a computer for imaging, allowing the neuronal activity of the living body to be observed through the imaging.

[0076] The microscope probe 400 may include a MEMS (Micro-Electro-Mechanical System) scanning galvanometer mirror and various lenses. Accordingly, the scanning control module may include a MEMS control module that controls the MEMS scanning galvanometer mirror.

[0077] The multiphoton microscope body according to the present disclosure integrates each functional module into a single integrated structure, significantly reducing the space required and making it suitable for various laboratories. The integrated structure also allows for neat and tidy wiring. The multiphoton microscope body is small in volume, portable, and easy to transport and move. The position and orientation of the multiphoton microscope can be quickly adjusted according to various testing needs, making it suitable for a wider range of applications. The multiphoton microscope body also facilitates rapid installation and maintenance in the field. The multiphoton microscope instrument according to the present disclosure may be a two-photon, three-photon, or nonlinear laser scanning microscope, such as Raman.

[0078] In one embodiment, the multiphoton microscope body 100 further includes a moving module 7 mounted on the mounting body 1, which can place a living organism on the moving module 7 and move the organism in multiple directions, and the wide-field search module 4 is configured to perform field search on the living organism located on the moving module 7.

[0079] For example, the living body can be directly attached to the moving module 7, and specifically, the living body can be restricted by providing a clamping or position restriction structure on the moving module 7. Alternatively, the living body can first be attached to the living body attachment device 8, and then the living body attachment device 8 can be fixed to the moving module 7. The moving module 7 can then move the living body attachment device 8 to adjust the position of the living body, allowing the wide-field search module 4 to image different regions of the living body and search for the target position of interest. The moving module 7 may be a multi-axis stage, capable of moving in multiple directions, such as up and down, left and right, and front and back.

[0080] The wide-field search module 4 is a device capable of wide-field imaging of a living body, and can employ single-photon fluorescence imaging. The image formed by the wide-field search module 4 can be transmitted to a computer for display, or an eyepiece can be attached to the wide-field search module 4 for direct observation.

[0081] 9, when wide-field imaging of a living body is performed using the wide-field search module 4, the living body is attached to a living body mounting device 8, and the living body mounting device 8 is fixed to the moving module 7. The living body may be a mouse or other animal.

[0082] FIG. 10 shows a living body mounting device 8 suitable for mounting a mouse (or other suitable animal). The living body mounting device 8 includes a mounting base 81, a treadmill 88, and a clamping mechanism, both of which are attached to the mounting base 81. The clamping mechanism includes two opposing clamping assemblies 82 that clamp both sides of a probe mounting member 80 attached to the mouse (the probe mounting member 80 is typically attached to the mouse's head; FIG. 10 only shows the probe mounting member 80, not the mouse). The clamping mechanism is configured to clamp and secure the probe mounting member 80 so that the mouse can run on the treadmill 88. The mounting base 81 is provided with bezels 811 positioned on both sides of the treadmill 88, which are configured to fasten the mouse to the treadmill 88. A fixing bracket 812, which is used to fix the mouse to the movement module 7, may be fixed to the mounting base 81 with bolts.

[0083] If the mouse runs on the treadmill 88 when the probe attachment member 80 attached to the mouse is clamped by the clamping assembly 82, the mouse's attention can be distracted, reducing the stress reaction of the mouse during the attachment process and advantageous for rapid experiments.

[0084] Optionally, each clamping assembly 82 includes two clamping sections and a first adjusting bolt 821. 821 are configured such that, by rotation, the two clamping parts are brought closer to each other to clamp the probe mounting member 80, or are moved apart to release the probe mounting member 80. Therefore, when clamping or removing the probe mounting member 80, it is only necessary to rotate the first adjustment bolts 821 of the two clamping assemblies 82, making attachment and detachment convenient.

[0085] Alternatively, the two clamping assemblies 82 are configured to be movable in the front-to-rear direction of the treadmill 88 relative to the mounting base 81 and also movable up and down relative to the mounting base 81. This allows adjustment so that the clamping assemblies 82 are positioned appropriately to clamp the probe mounting member 80 depending on the type or size of the living body.

[0086] Specifically, living organisms Installation The device 8 further includes a movable bracket 85 provided corresponding to each clamping assembly 82, and the movable bracket 85 is configured to be movable in the front-to-rear direction of the treadmill 88 relative to the mounting base 81, and the clamping assembly 82 is mounted on the movable bracket 85 so as to be able to move up and down. 10 As shown in FIG. 1, the moving bracket 85 is provided with a second adjustment bolt 86 configured to adjust the elevation of the clamping assembly 82; when the second adjustment bolt 86 is rotated, the second adjustment bolt 86 raises the clamping assembly 82, and when the second adjustment bolt 86 is rotated in the opposite direction, the clamping assembly 82 descends due to gravity. The mounting base 81 is provided with a third adjustment bolt 87 configured to adjust the forward and backward movement of the moving bracket 85. The third adjustment bolt 87 is threadedly engaged with the moving bracket 85, and when the third adjustment bolt 87 is rotated, the moving bracket 85 can be moved forward and backward relative to the mounting base 81.

[0087] Optionally, the living body InstallationThe device 8 further includes a light-shielding mechanism that shields the eyes of the living subject. The light-shielding mechanism includes a light-shielding cover 83 and a rotating member 84 that rotates the light-shielding cover 83. When performing wide-field search imaging on a mouse, by operating the rotating member 84, the light-shielding cover 83 can shield the mouse's eyes to protect them from the light rays.

[0088] living organisms Installation The apparatus 8 may further include a water dispenser (not shown) mounted on the mount 81, which is configured to provide drinking water to the mice on the treadmill, which is advantageous for distracting the mice.

[0089] living organisms Installation The device 8 further includes a tray 89 located below the mount 81 to receive mouse waste.

[0090] In one embodiment, as shown in FIGS. 7 and 9, the multiphoton microscope body further includes a field of view search adapter 9 attached to the mounting body 1, the field of view search adapter 9 including a probe mounting assembly 91 and a switching mechanism, the probe mounting assembly 91 being configured to detachably mount a microscope probe 400, and the switching mechanism switching the probe mounting assembly 91 between a first position and a second position. or It is configured to be switchable to a second position.

[0091] When the probe mounting assembly 91 is located in a first position, the microscope probe 400 attached to the probe mounting assembly 91 avoids the optical path between the wide-field search module 4 and the living body, and when the probe mounting assembly 91 is located in a second position, the microscope probe 400 is aligned with the optical path of the wide-field search module 4.

[0092] The switching mechanism may be configured to switch the probe mounting assembly 91 between two positions by manually pushing and pulling it. Specifically, the switching mechanism may be provided with an easy-to-hold grip (not shown), and when the probe mounting assembly 91 is pushed with the grip, the probe mounting assembly 91 moves to the first position, and when the probe mounting assembly 91 is pulled in the opposite direction, the probe mounting assembly 91 moves to the second position.

[0093] Furthermore, the field of view search adapter 9 may be provided with an objective lens 43, and when the field of view search adapter 9 is attached to the mounting body 1, the objective lens 43 is aligned with the optical path of the wide-field search module 4, and further, when the probe mounting assembly 91 is located at the second position, the microscope probe 400 attached thereto must be adjusted to be aligned with the optical path of the wide-field search module 4. When performing wide-field imaging using the wide-field search module 4, the switching mechanism switches the probe mounting assembly 91 to the first position, and after the wide-field search module 4 has located a target area in the living body, the probe mounting assembly 91 is switched to the second position, the microscope probe 400 is removed from the probe mounting assembly 91, and fixed (can be fixed by adhesive) at a position on the probe mounting member 80 of the living body corresponding to the located target area.

[0094] After the microscope probe 400 is attached to the probe attachment member 80, the living body (e.g., a mouse) is removed from the living body attachment device 8, and the mouse is released to move freely, thereby enabling neuronal observation to be performed on the freely moving mouse using the microscope probe 400.

[0095] In one embodiment, the multiphoton microscope main body 100 further includes an openable and closable light-shielding door 2 attached to the mounting main body 1. When the light-shielding door 2 is in a closed state, an enclosed space is formed between the mounting main body 1 and the light-shielding door 2, the moving module 7 is located within the enclosed space, and the wide-field searching module 4 is configured to perform wide-field searching on a living organism on the moving module 7 located within the enclosed space.

[0096] By providing the light-shielding door 2, it is possible to ensure that the living body is in a dark room when the wide-field search module 4 images the living body, thereby achieving a high imaging signal-to-noise ratio. Furthermore, by providing the light-shielding door 2, it is not necessary to create a dedicated light-shielding environment (for example, by providing a large cover or turning off the laboratory lights).

[0097] Alternatively, as shown in Figures 5 and 6, two light blocking doors 2 may be provided, adopting a double door structure, i.e., the two light blocking doors 2 are respectively rotatably attached to the mounting body 1, and close when rotated toward each other, and open when rotated away from each other. Figure 5 shows the state when the two light blocking doors 2 are closed, and Figure 6 shows the state when the two light blocking doors 2 are open.

[0098] Only one light-shielding door 2 may be provided, and may be configured to be opened and closed by lifting or sliding.

[0099] In one embodiment, the specific arrangement of each module of the multiphoton microscope main body 100 is as shown in FIGS. 7 and 8. The mounting main body 1 includes a base 11 and a mounting bracket 12 fixed to the base 11. A support plate 13 is provided at the upper position of the mounting bracket 12. The moving module 7 is mounted on the base 11. 11 The mounting bracket 12 is movably mounted on the mounting bracket 12 and is located on one side thereof, and a control box 5 located below a support plate 13 is fixed to the other side of the mounting bracket 12, the scanning control module and the fluorescence collection module are disposed in the control box 5, the laser light coupling module 3 is mounted on the support plate 13, and the wide-field search module 4 is mounted above the mobile module 7. The light-blocking door 2 is attached to the side of the mounting bracket 12 facing the mobile module 7 and is configured to form an enclosed space on the side of the mounting bracket 12 having the mobile module 7, and the optical path of the wide-field search module 4 located above can enter the enclosed space to image the living body on the mobile module 7.

[0100] Alternatively, the wide-field search module 4 is attached to the laser beam coupling module 3, and the laser beam coupling module 3 is provided with an optical path through-hole 331 that penetrates vertically, and the optical path of the wide-field search module 4 is configured to pass downward through the optical path through-hole 331 to reach the moving module 7. This makes the overall structure more compact and reduces the volume.

[0101] Optionally, the base 11 may be provided with a handle 14, which makes it possible to easily transport the multiphoton microscope main body 100.

[0102] Optionally, the base 11 may further be provided with a display 15, which can display, for example, the parameters of the laser light, the transmission status, the temperature and humidity of the multiphoton microscope, etc., so that the operating status of the equipment can be easily known.

[0103] In one embodiment, as shown in FIGS. 11 and 12, the laser beam coupling module 3 includes a coupler case 33, a dispersion compensation element 34, an acousto-optic modulator 36, and No. 2 an optical beam stabilization device, the dispersion compensation element 34, the acousto-optic modulator 36, and No. 2 The optical beam stabilizing devices are all provided in the coupler case 33 and are arranged sequentially along the direction of transmission of the laser light. The dispersion compensation element 34 is Transmission Transmission fiber optic 301 is configured to compensate for negative dispersion that occurs in the process of transmitting the laser light, and the acousto-optic modulator 36 is configured to adjust the intensity of the laser light, No. 2 The optical beam stabilization device is a laser beam coupling module 3. laser light The direction of laser light transmission is adjusted to correct for deviations between the actual and ideal positions of the laser light beams at the output end 32 . No. 2 The optical beam stabilizer is specifically positioned detector 39 may include:

[0104] The position detector 39 detects the position of the laser beam coupling module 3 laser light Located near the output end 32, laser light The position information of the laser beam at the output end 32 is detected. The position detector 39 can be a 4D position detector, which can strictly distinguish between position drift and angle drift of the light beam and accurately detect the real-time position of the light beam. The reflecting mirror adjustment mechanism can deflect the deflecting reflecting mirror based on the position information detected by the position detector 39 to adjust the transmission direction of the laser beam, so that the laser beam can be stably output from the output end to the laser beam transmission optical fiber and advantageously improve the coupling efficiency.

[0105] Specifically, first, laser light Determine an ideal position of the laser beam at the output end 32, the ideal position being: laser light This is the position where ideal coupling efficiency can be achieved when coupling laser light to the connected laser light transmission optical fiber 401 at the output end 32. When the light beam is deflected, for example, when the optical element is displaced due to vibration or temperature change, or when the light beam is deflected by an artificial touch, the position detector 39 detects the deflection. laser light The position information of the laser beam at the output end 32 is detected in real time and transmitted to the control unit. The control unit continuously determines the deviation between the position of the laser beam and the ideal position based on the position information, and controls the reflecting mirror adjusting mechanism to adjust the deflecting reflecting mirror to constantly adjust the reflection direction of the laser beam, so that the laser beam is stably transmitted to the laser beam transmitting optical fiber 401 within a certain range surrounding the ideal position. Conclusion It may be provided in the integrator case 33 or may be disposed in the control box 5.

[0106] Optionally, the laser light combining module 3 may further include at least one reflecting mirror 35 configured to change the laser light transmission direction. Changing the laser light transmission direction allows the light path to be bent, which facilitates the arrangement of each component in the light path and is advantageous for reducing the overall volume of the laser light combining module.

[0107] Specifically, as shown in FIG. 12, the laser light is incident on the laser beam coupling module 3. laser light The laser light enters from the input terminal 31, is dispersion compensated by the dispersion compensation element 34, is transmitted to the reflecting mirror 35, is deflected by about 90 degrees by the reflecting mirror 35, is transmitted to the acousto-optic modulator 36, and the intensity of the laser light is adjusted by the acousto-optic modulator 36, and then is 3 The beam is transmitted to the deflection mirror 37, 3 After being deflected by about 90 degrees by the deflection mirror 37, 4 The light is reflected by the deflection mirror 38. 4 By the deflecting mirror 38 laser light reflected to the output end 32, laser light The laser light is coupled to an optical fiber for transmitting laser light, which is connected to the output end 32. 3 polarized reflection mirror 37 and 4 The deflection mirrors 38 are provided with corresponding reflector adjustment mechanisms, and the deflection mirrors 38 are adjusted based on the position information detected by the position detector 39. 3 polarized reflection mirror 37 and 4 The position of the deflecting mirror 38 can be adjusted in real time, and the laser light can be output to the laser light transmission optical fiber 401 in a stable manner.

[0108] A power detector 311 may further be provided within the laser light combining module 3, configured to detect the laser light transmission power.

[0109] 13, the laser beam combining module further includes a driver 361 configured to drive the acousto-optic modulator 36 and a cooling mechanism configured to cool the driver 361, and the driver 361 and the cooling mechanism are both located on the upper surface of the combiner case 33. The cooling mechanism may include heat dissipation fins 362 that dissipate heat from the driver 361 and a fan 363 that dissipates heat from the heat dissipation fins 362.

[0110] The driver 361 has a large power for driving the radio frequency. ConclusionIf the driver 361 is placed inside the combiner case 33, there is an increased risk of interference from high-power radio frequency signals. Furthermore, the driver 361 dissipates a large amount of heat, which can easily deform the panels in the precision optical system, increasing the temperature inside the chamber and affecting the performance of the equipment. Therefore, the driver 361 is installed outside the combiner case 33, and heat dissipation fins 362 and a fan 363 are added to dissipate heat.

[0111] 13, the wide-field search module 4 is attached to the top of the coupler case 33, and the laser light coupling module 3 is provided with an optical path through-hole 331 that penetrates vertically, and the optical path of the wide-field search module 4 is configured to pass downward through the optical path through-hole 331. The wide-field search module 4 may include a fluorescent light source 41 and a camera 42, and the optical path of the camera 42 passes downward through the optical path through-hole 331 to reach the living body on the moving module 7, thereby realizing wide-field imaging of the living body.

[0112] The multi-photon microscope body may further include a cover 10 configured to cover the wide-field search module 4, the driver 361 and the cooling mechanism, and the cover 10 not only plays a protective role but also contributes to aesthetic appearance.

[0113] In one embodiment, as shown in Figures 14 and 15, the multi-photon microscope body further includes a control box 5 attached to the mounting body 1, and the fluorescence collection module and the scanning control module are both located in the control box 5.

[0114] The fluorescence collection module 53 may include a photomultiplier tube (PMT), and the signal collected by the fluorescence collection optical fiber 402 is conducted to the photomultiplier tube.

[0115] Optionally, the fluorescence collection module 53 may include a dichroic mirror and at least two spectral collection modules, each of which may include a photomultiplier tube. The fluorescence signal collected by the fluorescence collection optical fiber 402 from the microscope probe 400 is split into at least two fluorescence signals by the dichroic mirror, and then collected by the at least two spectral collection modules, respectively.

[0116] A signal processing module may be provided within the control box 5, and the signal processing module is configured to process the signal output from the fluorescence collection module 53 and transmit the processed signal to a computer for display. For example, the fluorescence signal collected by the fluorescence collection module 53 may be converted into an electrical signal, and after signal amplification, the signal may be collected and reorganized by high-speed AD collection, and then transmitted to a computer for display.

[0117] Both the fluorescence collection module and the signal processing module are installed in the control box 5, which shortens the transmission distance of the signal collected by the fluorescence collection module to the signal processing module, reduces the possibility of interference, and improves the reliability of signal transmission.

[0118] Optionally, the control box 5 is provided with a first interface 51 used to connect the control cable 403 and a second interface 52 used to connect the fluorescence collection optical fiber 402, and the first interface 51 and the second interface 52 are both located at an upper position on the same side of the control box 5.

[0119] The laser light coupling module 3 is located above the control box 5, and the output end of the laser light coupling module 3 used to connect the laser light transmission optical fiber 401 is located on the same side as the first interface 51 and the second interface 52 of the control box 5.

[0120] The laser beam coupling module 3 is provided above the control box 5, and the first interface 51 and the second interface 52 of the control box 5 Control box 5and the output end of the laser light coupling module 3 is located at a position above the first interface 51 and the second interface 52 of the control box 5, so that the laser light transmission optical fiber 401, the fluorescence collection optical fiber 402 and the control cable 403 can be placed close to each other, the wiring can be neatly arranged and the respective cables can be bundled together to form a bus cable, for example, by covering the bundled cables with a sheath to form a bus cable that can be easily stored in the storage device 6 (the storage of the cables and microscope probes by the storage device will be specifically described below).

[0121] Furthermore, by providing the lead-out ends of the laser light transmission optical fiber 401, the fluorescence collection optical fiber 402, and the control cable 403 at an upper position of the control box 5, it is possible to easily accommodate more behavioral devices and advantageously reduce the length of the cables. For example, when a mouse with a microscope probe 400 is placed in a living organism behavior box and allowed to move freely, the cable arrangement can make it easy for the microscope probe 400 to enter the living organism behavior box downwards.

[0122] 14 and 15, the control box 5 is provided with a main control circuit board 54 and a fluorescence collecting module 53, the fluorescence collecting module 53 is located above the main control circuit board 54, the main control circuit board 54 includes a scanning control module, and a laser light coupling module 3 (for example, an acousto-optic modulator for controlling the laser light coupling module 3, No. 2 Optical beam stabilizer), Wide field of view It may further include a control and driving circuit for controlling the search module 4, the indicator light, the light sensor, the temperature and humidity sensor, etc.

[0123] In one embodiment, the multi-photon microscope further includes a storage device 6, which is attached to the side of the control box 5 having the first interface 51 and the second interface 52, where the laser light transmission optical fiber 401, the fluorescence collection optical fiber 402, and the control cable 403 are all located. By attaching the storage device 6 to this side, the microscope probe 400 and the cables connected to the microscope probe 400, including the laser light transmission optical fiber 401, the fluorescence collection optical fiber 402, and the control cable 403, can be easily stored. To facilitate storage, the cables may be bundled and covered, for example, with a sheath.

[0124] As shown in Figures 16 and 17, the storage device 6 includes a storage body provided with a winding cylinder 633, around which an annular space is formed for storing the cable, and a probe holder 65 fixed to the storage body, in which the microscope probe 400 can be attached to the probe holder 65 after the cable is wound around the winding cylinder 633.

[0125] 16 shows a state in which the cable is wound around the winding cylinder 633 and the microscope probe 400 is attached to the probe holder 65. By storing the cable and probe in the storage device 6, the cable and probe are not easily damaged by being touched or pressed, and it is possible to prevent the cable from being tangled and knotted due to improper placement, or from being damaged due to improper bending.Furthermore, storing the cable and probe in the storage device 6 makes them neater, which is advantageous for improving the visual effect.

[0126] Specifically, the storage body includes a storage box 61 and a stopper disk 63 fixed to the storage box 61, and the probe holder 65 is fixed to the outside of the stopper disk 63 facing away from the storage box 61. The winding cylinder 633 is a part of the storage box 61 and the stopper disk 63 The storage box 61 and the stopper disk 63 are configured so that the cable wound around the winding cylinder 633 can be stopped from both ends of the winding cylinder 633.

[0127] 17, the stopper disk 63 includes a winding cylinder 633 and a stopper ring 632 that protrudes radially from the winding cylinder 633, and when the winding cylinder 633 is fixed to the storage box 61, an annular space that surrounds the winding cylinder 633 is formed between the stopper ring 632 and the storage box 61. Of course, the winding cylinder 633 may be formed directly on the storage box 61, and the stopper disk 63 is fixed to one end of the winding cylinder 633 that faces away from the storage box 61 and has the stopper ring 632 that protrudes radially from the winding cylinder 633.

[0128] The probe holder 65 may have multiple structural forms as long as it is capable of attaching the microscope probe 400. For example, the probe holder 65 may be configured to have an engagement structure that can engage the probe, and the probe holder 65 may be provided with an insertion hole for inserting the microscope probe 400.

[0129] Optionally, a plurality of position limiting notches are provided at intervals along the circumferential direction on the outer periphery of the stopper disc 63 (i.e., the stopper ring 632), and the cable extends from the winding cylinder 633 to the outside of the stopper disc 63 so that when the probe is attached to the probe holder 65, the cable is position-limited by one of the position limiting notches.

[0130] Alternatively, a clip ring 64 may be provided on the outer side of the stopper disk 63 facing away from the winding tube 633. The clip ring 64 may have a plurality of locking grooves 643 formed to surround the probe holder 65. The clip ring 64 may have a plurality of bumps spaced apart along the circumferential direction, with a locking groove 643 formed between each adjacent bump. The cable extends to the outside of the stopper disk 63 so that when the probe is attached to the probe holder 65, it can be locked in one of the locking grooves 643. This prevents the cable from loosening from the winding tube 633 and from swinging or loosening, which could cause the probe to separate from the probe holder. The clip ring 64 may be made of a flexible material, such as a rubber material, which has elasticity and can easily lock the cable in and out of the locking grooves.

[0131] In one embodiment, a through hole 612 is formed on the side of the storage box 61 that is attached to the control box 5, and the through hole 612 is connected to the annular space that stores the cable, and one end of the cable that has the microscope probe 400 can enter the storage box 61 through the through hole 612, extend into the annular space, and be wound around the winding cylinder 633.

[0132] Specifically, the storage box 61 is provided with a protrusion 611 that wraps around the through-hole 612 and protrudes toward the winding cylinder 633, an annular groove 613 is formed on the radially outer side of the protrusion 611 away from the through-hole 612, the winding cylinder 633 is provided on the stopper disk 63, the winding cylinder 633 is fixed to the protrusion 611, and the annular groove 613 forms an annular space for winding the cable between the storage box 61 and the stopper disk 63. The protrusion 611 may be configured to have an annular structure or an arc-shaped structure having a notch, and in order to attach the winding cylinder 633, a male thread may be provided on the outer surface of the protrusion 611 and a female thread may be provided on the inner surface of the winding cylinder 633, or the winding cylinder 633 may be connected to the protrusion 611 of the storage box 61 by a screw connection.

[0133] The protrusion 611 is provided with a through groove 614 that penetrates the protrusion 611 in the radial direction, and one end of the cable having the microscope probe 400 can enter the storage box 61 through the through hole 612 and then extend outward from the through groove 614 into the annular space. The through groove 614 may be a notch formed in the protrusion 611 as shown in FIG. 13 , or may be a through hole provided in the wall of the protrusion 611. When the winding cylinder 633 is fitted and fixed to the protrusion 611, the through groove 614 is located approximately at one end of the winding cylinder 633, and the cable can extend from the through groove 614 into the annular space and be wound around the winding cylinder 633.

[0134] In one embodiment, the storage device further includes a ring-shaped indicator light 62, which is attached to the storage body and arranged around the center of the winding tube 633. The ring-shaped indicator light 62 may be arranged to illuminate the interior of the storage device 6 or to indicate the operating state of the multiphoton microscope body. For example, the ring-shaped indicator light 62 may be arranged to indicate, with different colors, whether the device is in an operating state, an inoperable state, a malfunction, or an abnormality. For example, when the controller detects that laser light has entered the laser beam coupling module 3 or that the laser device is emitting laser light, the controller controls the ring-shaped indicator light 62 to display green, which indicates that the device is in an operating state. When the controller detects an abnormality in the device, such as a laser beam power abnormality or other abnormal state, the controller controls the ring-shaped indicator light 62 to display red. When the device is in an inoperable state, the ring-shaped indicator light 62 may display yellow and be used for interior illumination.

[0135] The stopper disk 63 includes a light-transmitting cover 631, and the annular indicator light 62 is provided between the storage box 61 and the stopper disk 63 and corresponds to the light-transmitting cover 631, so that the light emitted by the annular indicator light 62 can be seen through the light-transmitting cover 631. The stopper ring 632 of the stopper disk 63 is provided on the outer ring of the light-transmitting cover 631.

[0136] The storage device 6 may further include a protective lid 66 covering the outside of the storage body, and the protective lid 66 may be rotatably attached to the storage body by a rotation shaft 67 and a hinge 68, and may be provided so as to be rotatable between an open state and a closed state. A magnet may be provided between the protective lid 66 and the storage body to hold the protective lid 66 in the closed state.

[0137] protective lid 66 The annular lid 661 and the transparent observation window provided in the center of the annular lid 661 are 662 , so that the interior of the storage device can be easily observed and the equipment status indicated by the annular indicator light 62 can be easily observed.

[0138] When in use, the protective cover 66 is rotated until it opens, the microscope probe 400 is removed from the probe holder 65, and the cable is unwound from the winding cylinder 633, and the probe can be attached to a living body and used.

[0139] A further aspect of the present disclosure is Multiphoton Microscope System 18 and 19, the multiphoton microscope system includes a laser device 200, a microscope probe 400, and the above-mentioned multiphoton microscope main body 100, wherein the laser device 200 is configured to transmit laser light to a laser light coupling module 3, the output end of the laser light coupling module 3 is connected to the microscope probe 400 via a laser light transmission optical fiber 401, the fluorescence collection module is connected to the microscope probe 400 via a fluorescence collection optical fiber 402, and the scanning control module is connected to the microscope probe 400 via a control cable 403.

[0140] In one embodiment, as shown in FIG. 18, the optical system further includes a laser light adapter 300, and the laser device 200 first emits laser light into the laser light adapter 300, and the laser light is adjusted and adapted by the laser light adapter 300, and then transmitted to the multiphoton microscope main body 100 via a transmission optical fiber 301.

[0141] By providing a laser light adapter 300 between the laser device 200 and the multiphoton microscope main body 100, laser light with different parameters emitted by various laser devices 200 can be adjusted by the laser light adapter 300 and then adapted to the subsequent device. Furthermore, because the laser light adapter 300 and the multiphoton microscope main body 100 are connected by a transmission optical fiber 301, the multiphoton microscope 100 can move freely and be placed in different positions as needed, and can even be installed across stages, allowing for more flexible use.

[0142] Specifically, the laser light adapter 300 may include a case and a beam converter located within the case. The beam converter is configured to convert the laser light beam entering the case. The beam converter refers to using the conversion characteristics of an optical element to perform operations such as expanding and contracting the beam and changing the focal length, thereby matching the laser with downstream equipment and optimizing its performance. The beam stabilizer is located downstream of the beam converter in the laser light transmission direction and adjusts the deflection direction of the laser light beam to correct deviations between the actual and ideal positions of the laser light beam at the laser light output port. The beam stabilizer is similar to the beam converter installed in the laser light coupling module 3. When a deviation in the laser light beam is detected, the beam stabilizer can adjust the deflection direction of the laser light to stabilize the laser light output and ensure the coupling efficiency of the laser light output.

[0143] In the embodiment shown in FIG. 19, a fixed optical path may be provided between the laser device 200 and the multiphoton microscope main body 100. Device 200 and the multiphoton microscope main body 100 cannot be moved, and if one attempts to change the position, it is necessary to rearrange the optical path.

[0144] The above are merely preferred embodiments of the present disclosure, and are not intended to limit the present disclosure. Any modifications, equivalent replacements, etc. made within the spirit and principle of the present disclosure should be included in the protection scope of the present disclosure. [Explanation of symbols]

[0145] 100 Multiphoton microscope body 1 Mounting body 11 Pedestal 12 Mounting bracket 13 Support plate 14 Handle 15 Display 2 Light-blocking doors 3 Laser optical coupling module 31 laser light Input terminal 32 laser light Output terminal 311 Power Detector 33 Coupler case 331 Optical path through hole 34 Dispersion compensation element 35 Reflector 36 Acousto-optic modulator 361 Driver 362 Heat dissipation fin 363 fans 37th 3 polarized reflector 38th 4 polarized reflector 39 Location detector 4 Wide Visual Search Module 41 Fluorescent light source 42 Camera 43 Objective Lens 5 Control box 51 First Interface 52 Second Interface 53 Fluorescence Collection Module 54 Main control circuit board 6 Storage device 61 Storage Box 611 Protrusion 612 Through hole 613 Annular groove 614 Through groove 62 Ring indicator light 63 Stopper disk 631 Translucent cover 632 Stopper Ring 633 Rolling cylinder 64 Clip Ring 643 Locking groove 65 Probe holder 66 Protective lid 661 Annular lid 662 Peephole 67 Rotation axis 68 Hinge 7. Mobile Module 8. Bio-attachment Device 81 Mounting base

Claims

1. 1. A laser light adapter, comprising: a case; and an optical beam transforming device and an optical beam stabilizing device provided in the case; the case has a laser light input port and a laser light output port; the light beam transformation device is configured to transform a laser light beam that enters the case; the light beam stabilizing device is provided downstream of the light beam transforming device in the laser light transmission direction, and is used to adjust the laser light transmission direction so as to correct a deviation between the actual position of the laser light beam at the laser light output port and an ideal position thereof; Laser light adapter.

2. the optical beam stabilization device includes a position detector, at least one deflection mirror, and a mirror adjustment mechanism connected to each of the deflection mirrors; the position detector is provided at a position close to the laser light output port and is used to detect position information of the laser light at the laser light output port; the reflecting mirror adjustment mechanism is configured to be able to deflect the deflecting reflecting mirror based on position information detected by the position detector in order to adjust the laser light transmission direction.

2. The laser light adapter according to claim 1.

3. the laser light adapter further includes a control unit, the control unit receiving position information detected by the position detector and controlling the reflector adjustment mechanism based on the position information; 3. The laser light adapter according to claim 2.

4. the laser light adapter further includes at least one fixed reflector used to change the laser light transmission direction, the fixed reflector being disposed upstream of the light beam transforming device in the laser light transmission direction; 4. The laser light adapter according to claim 2 or 3.

5. the at least one fixed reflector includes a first fixed reflector and a second fixed reflector, and the at least one deflecting reflector includes a first deflecting reflector and a second deflecting reflector; the laser light is reflected by the first fixed reflecting mirror to the second fixed reflecting mirror, the second fixed reflecting mirror reflects the laser light to the light beam conversion device, the laser light emitted from the light beam conversion device is reflected by the first deflection reflecting mirror to the second deflection reflecting mirror, and the second deflection reflecting mirror reflects the laser light to the laser light output port; 5. The laser light adapter according to claim 4.

6. At least one laser light power meter is provided in the case and is used to detect the power of the laser light entering the laser light adapter.

6. The laser light adapter according to claim 1.

7. the at least one laser beam power meter includes a first laser beam power meter and a second laser beam power meter, the first laser beam power meter being provided at a position close to the laser beam input port, and the second laser beam power meter being provided at a position close to the laser beam output port; 7. The laser light adapter according to claim 6.

8. the laser light adapter further includes a switch device provided at the laser light input port, the switch device including an opening / closing door used to open and close the laser light input port, and a door drive mechanism that drives the opening / closing door to switch between an open state and a closed state; When the door is opened, the laser light enters the laser light adapter and is transmitted, and when the door is closed, the laser light is prevented from entering the laser light adapter.

8. The laser light adapter according to claim 1.

9. Support legs are provided below the case, and the support legs are configured to be height adjustable.

9. The laser light adapter according to claim 1.

10. 1. An optical system comprising: A laser device, an application device body, and a laser light adapter according to any one of claims 1 to 9, the laser device is configured to emit a laser beam into the laser beam adapter; the laser light adapter is configured to receive the laser light emitted by the laser device, adjust and adapt the laser light, and then transmit the adjusted laser light to the application device body; The application device main body is configured to transmit laser light to a microscope probe and control the microscope probe to perform laser scanning on a living body to generate a fluorescent signal for imaging. Optical system.

11. the optical system further includes a transmission optical fiber connected between the laser light adapter and the application device body, the laser light adapter transmitting the conditioned laser light from the transmission optical fiber to the application device body; 11. The optical system of claim 10.

12. The application device body includes a laser beam coupling module, the laser beam coupling module having a laser beam input end connected to the transmission optical fiber and a laser beam output end connected to the microscope probe via the laser beam transmission optical fiber; the laser light coupling module is configured to condition the laser light received from the transmission optical fiber and then transmit it to the microscope probe via the laser light transmission optical fiber; 12. The optical system of claim 11.

13. the laser light coupling module includes a power detector used to detect laser light power; 13. The optical system of claim 12.

14. the laser light power meter is provided near the laser light output end of the laser light adapter, and the power detector is provided near the laser light input end of the laser light coupling module; 14. The optical system of claim 13.

15. The application device body includes a multiphoton microscope body; the multiphoton microscope body is configured to connect to the microscope probe, and includes a mounting body, a wide-field search module, a laser light coupling module, a fluorescence collection module, and a scan control module integrated in the mounting body; the wide-field search module is configured to perform wide-field imaging on the living body to search for a target area in the living body for attaching a microscope probe; the laser light coupling module is configured to receive laser light and condition the laser light to couple the laser light into a laser light transmission optical fiber, the laser light transmission optical fiber being configured to connect the laser light coupling module and the microscope probe; the scanning control module is connected to the microscope probe via a control cable and configured to control the microscope probe to laser scan and generate a fluorescent signal; the fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection optical fiber and configured to collect a fluorescence signal output by the microscope probe; The optical system according to any one of claims 10 to 14.

16. The multiphoton microscope further includes a field-of-view search adapter attached to the mounting body; the field-searching adapter includes a probe mounting assembly and a switching mechanism, the probe mounting assembly configured to removably mount the microscope probe, and the switching mechanism configured to switch the probe mounting assembly between a first position and a second position; When the probe mounting assembly is located at the first position, the microscope probe mounted on the probe mounting assembly avoids an optical path between the wide-field search module and the living body, and when the probe mounting assembly is located at the second position, the microscope probe is aligned with the optical path of the wide-field search module.

16. The optical system of claim 15.

17. The multiphoton microscope main body further includes a moving module provided on the mounting body, the moving module being configured to place a living body thereon and move the living body in a plurality of directions, and the wide-field search module being configured to perform a wide-field search on the living body positioned on the moving module.

17. An optical system according to claim 15 or 16.

18. the multiphoton microscope body further includes an openable and closable light-shielding door attached to the mounting body; When the light-shielding door is in a closed state, a sealed space is formed between the mounting body and the light-shielding door, and the moving module is located in the sealed space; The wide-field search module is configured to perform wide-field search on a living body on the mobile module located in an enclosed space.

18. The optical system of claim 17.

19. The mounting body includes a base and a mounting bracket fixed to the base, and a support plate is provided at an upper position of the mounting bracket. the moving module is movably mounted on the base and is located on one side of the mounting bracket, and a control box is mounted on the other side of the mounting bracket and is located below the support plate, the scanning control module and the fluorescence collecting module are provided in the control box; the laser beam coupling module is attached above the support plate, and the wide-field search module is attached above the moving module; 19. An optical system according to claim 17 or 18.

20. The wide-field search module is attached to the laser beam coupling module, and the laser beam coupling module is provided with an optical path through-hole penetrating vertically, and the optical path of the wide-field search module is configured to pass through the optical path through-hole downward and reach the moving module.

20. An optical system according to claim 18 or 19.

21. the laser beam combining module includes a combiner case, a dispersion compensation element, an acousto-optic modulator, and an optical beam stabilizing device; the dispersion compensation element, the acousto-optic modulator, and the optical beam stabilizing device are all provided within the coupler case, and are provided sequentially along the transmission direction of the laser light; the dispersion compensation element is configured to compensate for negative dispersion that occurs in the process of transmitting laser light through the transmission optical fiber; the acousto-optic modulator is configured to adjust the intensity of the laser light; the light beam stabilizing device is configured to adjust the laser light transmission direction to correct a deviation between an actual position and an ideal position of the laser light beam at the laser light output end of the laser light combining module; The optical system according to any one of claims 15 to 20.

22. the laser beam coupling module further includes a driver used to drive the acousto-optic modulator and a cooling mechanism used to cool the driver, the driver and the cooling mechanism both being located on an upper surface of the coupler case; 22. The optical system of claim 21.

23. the wide-field search module is attached to the top surface of the coupler case, the laser light coupling module is provided with an optical path through hole penetrating vertically, and the optical path of the wide-field search module is configured to pass through the optical path through hole downward; the multiphoton microscope main body further includes a cover lid used to cover the wide-field search module, the driver, and the cooling mechanism; 23. The optical system of claim 22.

24. The multiphoton microscope body further includes a control box attached to the mounting body, and the fluorescence collection module and the scanning control module are both located within the control box. The optical system according to any one of claims 15 to 23.

25. the control box is provided with a first interface used to connect the fluorescence collection optical fiber and a second interface used to connect the control cable, the first interface and the second interface both being located at an upper position on the same side of the control box; the laser light coupling module is located above the control box, and an output end of the laser light coupling module used to connect the laser light transmission optical fiber is located on the same side of the control box as the first interface and the second interface; 25. The optical system of claim 24.

26. One side of the first interface and the second interface, the storage device is used to store the microscope probe and cables connected to the microscope probe, including the laser light transmission optical fiber, the fluorescence collection optical fiber, and the control cable; 26. The optical system of claim 25.

27. The work table further includes a work table body and a display; The worktable body is connected to the microscope body, and the microscope body processes the collected fluorescent signal and then transmits it to the worktable body, and the display displays the formed image; The worktable body further transmits a control command to the microscope body. The optical system according to any one of claims 10 to 26.

28. A multiphoton microscope body configured to connect to a microscope probe, a mounting body; a wide-field search module, a laser beam coupling module, a fluorescence collection module, and a scan control module integrated in the mounting body; the wide-field search module is configured to perform wide-field imaging on the living body to search for a target area in the living body for attaching a microscope probe; the laser light coupling module is configured to receive laser light and condition the laser light to couple the laser light into a laser light transmission optical fiber, the laser light transmission optical fiber being configured to connect the laser light coupling module and the microscope probe; the scanning control module is connected to the microscope probe via a control cable and configured to control the microscope probe to laser scan and generate a fluorescent signal; the fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection optical fiber and configured to collect a fluorescence signal output by the microscope probe; Multiphoton microscope body.

29. The multiphoton microscope further includes a field-of-view search adapter attached to the mounting body; the field-searching adapter includes a probe mounting assembly and a switching mechanism, the probe mounting assembly configured to removably mount the microscope probe, and the switching mechanism configured to switch the probe mounting assembly between a first position and a second position; When the probe mounting assembly is located at the first position, the microscope probe mounted on the probe mounting assembly avoids an optical path between the wide-field search module and the living body, and when the probe mounting assembly is located at the second position, the microscope probe is aligned with the optical path of the wide-field search module.

29. The multiphoton microscope body according to claim 28.

30. The multiphoton microscope main body further includes a moving module provided on the mounting body, the moving module is used to place a living body and can move the living body in multiple directions, and the wide-field search module is configured to perform field search on the living body located on the moving module.

30. A multiphoton microscope body according to claim 28 or 29.

31. the multiphoton microscope body further includes an openable and closable light-shielding door attached to the mounting body; When the light-shielding door is in a closed state, a sealed space is formed between the mounting body and the light-shielding door, and the moving module is located in the sealed space; The wide-field search module is configured to perform wide-field search on a living body on the mobile module located in an enclosed space. The multiphoton microscope body according to claim 30.

32. The mounting body includes a base and a mounting bracket fixed to the base, and a support plate is provided at an upper position of the mounting bracket. the moving module is movably mounted on the base and is located on one side of the mounting bracket, and a control box is mounted on the other side of the mounting bracket and is located below the support plate, the scanning control module and the fluorescence collecting module are provided in the control box; the laser beam coupling module is attached above the support plate, and the wide-field search module is attached above the moving module; 32. The multiphoton microscope body according to claim 30 or 31.

33. The wide-field search module is attached to the laser beam coupling module, and the laser beam coupling module is provided with an optical path through-hole penetrating vertically, and the optical path of the wide-field search module is configured to pass through the optical path through-hole downward and reach the moving module.

33. A multiphoton microscope body according to claim 31 or 32.

34. the laser beam combining module includes a combiner case, a dispersion compensation element, an acousto-optic modulator, and an optical beam stabilizing device; the dispersion compensation element, the acousto-optic modulator, and the optical beam stabilizing device are all provided within the coupler case, and are provided sequentially along the transmission direction of the laser light; the dispersion compensation element is used to compensate for negative dispersion that occurs in the process of transmitting laser light through the transmission optical fiber; the acousto-optic modulator is used to adjust the intensity of the laser light; the light beam stabilizing device adjusts the laser light transmission direction to correct the deviation between the actual position and the ideal position of the laser light beam at the laser light output end of the laser light combining module; The multiphoton microscope body according to any one of claims 28 to 33.

35. the laser beam coupling module further includes a driver used to drive the acousto-optic modulator and a cooling mechanism used to cool the driver, the driver and the cooling mechanism both being located on an upper surface of the coupler case; The multiphoton microscope body according to claim 34.

36. the wide-field search module is attached to the top surface of the coupler case, the laser light coupling module is provided with an optical path through hole penetrating vertically, and the optical path of the wide-field search module is configured to pass through the optical path through hole downward; the multiphoton microscope main body further includes a cover lid used to cover the wide-field search module, the driver, and the cooling mechanism; The multiphoton microscope body according to claim 35.

37. The multiphoton microscope body further includes a control box attached to the mounting body, and the fluorescence collection module and the scanning control module are both located within the control box. The multiphoton microscope body according to any one of claims 28 to 36.

38. the control box is provided with a first interface used to connect the fluorescence collection optical fiber and a second interface used to connect the control cable, the first interface and the second interface both being located at an upper position on the same side of the control box; the laser light coupling module is located above the control box, and an output end of the laser light coupling module used to connect the laser light transmission optical fiber is located on the same side of the control box as the first interface and the second interface; 38. The multiphoton microscope body according to claim 37.

39. a storage device attached to the control box on a side having the first interface and the second interface; the storage device is used to store the microscope probe and cables connected to the microscope probe, including the laser light transmission optical fiber, the fluorescence collection optical fiber, and the control cable; 39. The multiphoton microscope body according to claim 38.

40. 1. A multiphoton microscope system, comprising: A laser device, a microscope probe, and a multiphoton microscope body according to any one of claims 28 to 39, the laser device is configured to transmit laser light to the laser light coupling module, the output end of the laser light coupling module is connected to the microscope probe via a laser light transmitting optical fiber, the fluorescence collecting module is connected to the microscope probe via a fluorescence collecting optical fiber, and the scanning control module is connected to the microscope probe via a control cable; Multiphoton microscope system.

41. 1. An optical imaging system comprising: The laser device includes a laser light adapter and a microscope body. the laser device is configured to emit a laser beam into the laser beam adapter; the laser light adapter is configured to receive the laser light emitted by the laser device, adjust and adapt the laser light, and then transmit the adjusted laser light to the microscope body; The microscope body is configured to transmit laser light to a microscope probe and control the microscope probe to perform laser scanning on a living body to generate a fluorescent signal for imaging. Optical imaging system.

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