System for shaping a reinforcement layer, method for shaping a reinforcement layer, and program

Abstract

A system for shaping a reinforcement layer is provided that allows for uniform formation of the laminate. [Solution] The plastic surgery system 100 has a detection unit 121 that detects the thickness of a reinforcing layer made of fibrin gel formed on a sheet-shaped cell culture 10, a resection unit 130 that excises the reinforcing layer, a calculation unit 123 that calculates the height of the excision unit when the reinforcing layer in a region 30H where the thickness of the reinforcing layer is equal to or greater than a predetermined value is excised by the resection unit, and a control unit 120 that controls the excision unit to move horizontally from the height calculated by the calculation unit, thereby excising the reinforcing layer in the region where the thickness of the reinforcing layer is equal to or greater than the predetermined value.

Description

[Technical Field]

[0001] The present invention relates to a system for shaping a reinforcement layer, a method for shaping a reinforcement layer, and a program. [Background technology]

[0002] Sheet-shaped cell cultures (cell sheets) are widely known for use in fields such as regenerative medicine. Sheet-shaped cell cultures are produced by storing and culturing cells collected from humans or non-human animals in a culture substrate (culture vessel) containing a culture medium, and allowing them to adhere and aggregate into a sheet.

[0003] Because the sheet-shaped cell culture is constructed in the form of a thin film sheet, it is physically fragile and therefore requires careful handling, as it is prone to wrinkling and tearing during isolation from the culture substrate, transportation and storage of the sheet-shaped cell culture, and actual transplantation into a patient's body.

[0004] To solve the above problems, a method (fibrin treatment) has been proposed in which a fibrinogen solution and a thrombin solution are applied to a sheet-shaped cell culture, a reinforcing layer made of fibrin gel is formed on the sheet-shaped cell culture, and a laminate consisting of the sheet-shaped cell culture and the reinforcing layer is produced (see, for example, Patent Document 1). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent Publication No. 2014-179151 Summary of the Invention [Problem to be solved by the invention]

[0006] On the other hand, when forming a reinforcing layer made of fibrin gel on a sheet-shaped cell culture and producing a laminate consisting of the sheet-shaped cell culture and the reinforcing layer, areas where the thickness of the laminate is greater than a predetermined value may unintentionally occur, resulting in an uneven thickness of the laminate.

[0007] When there are regions where the thickness of the laminate is uneven, the laminate is difficult to handle, and the sheet-shaped cell culture may be damaged or may be difficult to transplant into the target location.

[0008] An object of the present invention is to provide a system for shaping a reinforcing layer, a method for shaping a reinforcing layer, and a program that enable a laminate to be formed uniformly. [Means for solving the problem]

[0009] The present invention is achieved by any one of the following means (1) to (7).

[0010] (1) a detection unit for detecting the thickness of a reinforcing layer made of fibrin gel formed on the sheet-shaped cell culture; a cutting portion for cutting the reinforcing layer; a calculation unit that calculates a height of the cutting portion when cutting the reinforcing layer in a region where the thickness of the reinforcing layer is equal to or greater than a predetermined value; A system for shaping a reinforcement layer, comprising: a control unit that controls the cutting unit to move horizontally from the height calculated by the calculation unit, thereby cutting out the reinforcement layer in areas where the thickness of the reinforcement layer is greater than or equal to a predetermined value.

[0011] (2) an imaging unit for imaging the sheet-shaped cell culture and the reinforcing layer, The system for shaping a reinforcing layer according to (1), wherein the detection unit detects the thickness of the reinforcing layer based on image information acquired by the imaging unit.

[0012] (3) a light irradiation unit that irradiates the sheet-shaped cell culture and the reinforcing layer with an inspection light; The system for shaping a reinforcing layer described in (1) or (2), wherein the detection unit detects the thickness of the reinforcing layer based on the light transmittance of the inspection light through the sheet-shaped cell culture and the reinforcing layer.

[0013] (4) The system for shaping a reinforcing layer according to any one of (1) to (3), wherein the calculation unit also calculates a horizontal position when the cutting unit cuts off the reinforcing layer.

[0014] (5) The system for shaping a reinforcing layer according to any one of (1) to (4), further comprising a processing unit for forming the reinforcing layer on the sheet-shaped cell culture.

[0015] (6) a step in which a detection unit detects the thickness of a reinforcing layer made of fibrin gel formed on the sheet-shaped cell culture; a step in which a calculation unit calculates a height of a cutting portion when cutting the reinforcing layer in a region where the thickness of the reinforcing layer is equal to or greater than a predetermined value; A method for shaping a reinforcement layer, comprising a step of moving the cutting horizontally from the height calculated by the calculation unit to cut out the reinforcement layer in an area where the thickness of the reinforcement layer is equal to or greater than a predetermined value.

[0016] (7) A system having a resection unit that resects a region of a reinforcing layer made of fibrin gel where the thickness of the reinforcing layer is equal to or greater than a predetermined value, detecting a thickness of the reinforcing layer; calculating a height of the cutout portion when cutting out the reinforcing layer in a region where the thickness of the reinforcing layer is equal to or greater than a predetermined value; and controlling the cutting unit to cut off the reinforcing layer at a calculated height in an area where the thickness of the reinforcing layer is equal to or greater than a predetermined value. [Effects of the Invention]

[0017] According to the system, method, and program for shaping a reinforcing layer of the present invention, the detection unit detects the thickness of the reinforcing layer, the calculation unit calculates the height of the reinforcing layer to be cut by the cutting unit in areas where the reinforcing layer thickness is equal to or greater than a predetermined value, and the cutting unit moves horizontally from the height calculated by the calculation unit to cut the reinforcing layer in areas where the reinforcing layer thickness is equal to or greater than the predetermined value. As a result, it is possible to eliminate areas where the reinforcing layer thickness is equal to or greater than the predetermined value, thereby forming a uniform laminate. [Brief explanation of the drawings]

[0018] [Figure 1] FIG. 1 is a block diagram illustrating a system for shaping a reinforcement layer, according to an embodiment. [Figure 2] 1 is a flowchart illustrating steps in a method for shaping a reinforcement layer, according to an embodiment. [Figure 3] FIG. 10 is a cross-sectional view for explaining a method for shaping a reinforcing layer according to an embodiment, showing the state before excess reinforcing layer is removed by a removal section. [Figure 4] 10 is a cross-sectional view for explaining a method for shaping a reinforcing layer according to an embodiment, showing how an excess reinforcing layer is cut off by a cutting portion. FIG. [Figure 5] FIG. 10 is a cross-sectional view for explaining a method for shaping a reinforcing layer according to an embodiment, showing the state after excess reinforcing layer has been removed by a removal section. DETAILED DESCRIPTION OF THE INVENTION

[0019] Hereinafter, embodiments of the present invention will be described with reference to the drawings. Note that the dimensional proportions in the drawings are exaggerated for the sake of explanation and may differ from the actual proportions.

[0020] FIG. 1 is a simplified block diagram showing the overall configuration of a system 100 for shaping the reinforcing layer 30 according to the present embodiment (hereinafter also referred to as the "shaping system 100"). FIG. 2 is a flowchart showing each step of a method for shaping the reinforcing layer 30 according to the present embodiment (hereinafter also referred to as the "shaping method"). FIG. 3 is a cross-sectional view for explaining the method for shaping the reinforcing layer 30 according to the embodiment, showing the state before excess reinforcing layer 30 is removed by a cutting unit 130. FIG. 4 is a cross-sectional view for explaining the method for shaping the reinforcing layer 30 according to the embodiment, showing the state after excess reinforcing layer 30 has been removed by a cutting unit 130. FIG. 5 is a cross-sectional view for explaining the method for shaping the reinforcing layer 30 according to the embodiment, showing the state after excess reinforcing layer 30 has been removed by a cutting unit 130.

[0021] Arrows X1 and X2 in each figure indicate directions parallel to the surface direction of the sheet-shaped cell culture 10, and arrows Z1 and Z2 indicate the thickness direction perpendicular to the surface direction of the sheet-shaped cell culture 10 and the laminate 20.

[0022] <Sheet-shaped cell culture 10> The sheet-shaped cell culture 10 used in this embodiment can contain any cells capable of forming the sheet-shaped cell culture 10. The specific types of such cells are not particularly limited, but include, for example, somatic stem cells (adult stem cells), mesenchymal stem cells, or iPS cell (induced pluripotent stem cell)-derived cardiomyocytes, fibroblasts, synovial cells, epithelial cells, and endothelial cells. Somatic stem cells preferably include skeletal myoblasts (myoblast cells). Furthermore, the term "sheet-shaped cell culture" as used herein refers to a sheet-like (thin film-like) structure in which cells are interconnected.

[0023] The sheet-shaped cell culture 10 may be configured to have, for example, a substantially circular planar shape in its natural state when no external force is applied. The planar shape of the sheet-shaped cell culture 10 is not limited to a circle, and may be, for example, a rectangle, a triangle, an oval, or any other geometric shape. There are also no particular limitations on the specific size or thickness of the sheet-shaped cell culture 10.

[0024] 3 to 5, the sheet-shaped cell culture 10 has a surface 10a, a back surface 10b located opposite the surface 10a and facing the bottom 223 of the container 220, and a peripheral edge 10c having a circular outer shape. The thickness of the sheet-shaped cell culture 10 is, for example, 100 to 1000 μm.

[0025] The shaping method described below can be carried out while the sheet-shaped cell culture 10 is contained in a predetermined container 220. The target sheet-shaped cell culture 10 is generally in a state after being detached from the container. The target sheet-shaped cell culture 10 may be a single layer or a laminate of multiple sheets. The target sheet-shaped cell culture 10 may have thickness variations.

[0026] The container 220 is, for example, a culture substrate used for culturing the sheet-shaped cell culture 10. When the container 220 is a culture substrate, the container 220 can be made of a material that is impermeable to liquids such as liquid culture media. When the container 220 is a culture substrate, the solution (liquid culture media, etc.) used for culturing is discharged from the container 220 before starting the shaping method.

[0027] As shown in Figures 3 to 5, the container 220 has an opening 221 that opens upward, a bottom 223 on which the back surface 10b of the sheet-shaped cell culture 10 is placed, and a side wall 225 that surrounds the periphery of the bottom 223.

[0028] The container 220 has a substantially circular planar shape. The bottom 223 of the container 220 has a size (inner diameter) that is the same as or larger than that of the sheet-shaped cell culture 10.

[0029] There are no particular restrictions on the specific form of the sheet-shaped cell culture 10 (e.g., manufacturing method, physical properties, function, etc.) and the specific form of the culture substrate (e.g., material, function, structure and shape, type of liquid medium used, etc.), and publicly known content (e.g., Patent Publication No. 2011-155869, Patent Publication No. 2022-106986, Patent Publication No. 2021-106609, etc.) can be taken into consideration as appropriate.

[0030] <Orthopedic System 100> To summarize with reference to Figure 1, the plastic surgery system 100 comprises a processing unit 110 that performs processing to form a reinforcing layer 30 made of fibrin gel on the sheet-shaped cell culture 10, a detection unit 121 that detects the thickness of the reinforcing layer 30, an excision unit 130 that excises the reinforcing layer 30, a calculation unit 123 that calculates the height of the excision unit 130 when the excision unit 130 cuts the reinforcing layer 30 in region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value, and a control unit 120 that controls the excision unit 130 to move horizontally from the height calculated by the calculation unit 123, thereby excising the reinforcing layer 30 in region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value.

[0031] As shown in Figure 1, the shaping system 100 may further include an imaging unit 140 that photographs the sheet-shaped cell culture 10 and fibrin gel, a stage 210 on which a container 220 containing the sheet-shaped cell culture 10 can be placed, an image display unit 150 that can display an image generated from image information (image data) acquired by the imaging unit 140, and a light irradiation unit 160 that irradiates the laminate 20 with inspection light f.

[0032] As shown in Figure 1, the processing unit 110 can be configured to have a first application unit 111 that applies fibrinogen liquid (liquid containing fibrinogen) L1 to the sheet-shaped cell culture 10, and a second application unit 112 that applies thrombin liquid (liquid containing thrombin) L2 to the sheet-shaped cell culture 10.

[0033] The first applicator 111 can be configured to include, for example, a mechanism for dripping the fibrinogen liquid L1 onto the sheet-shaped cell culture 10. Examples of the mechanism for dripping the fibrinogen liquid L1 include a known syringe or pipette.

[0034] The second applicator 112 can be configured to include, for example, a mechanism for spraying the thrombin solution L2 onto the sheet-shaped cell culture 10. The mechanism for spraying the thrombin solution L2 can be, for example, a known spray.

[0035] The fibrinogen solution L1 and the thrombin solution L2 are not particularly limited as long as they react with each other and solidify to form a fibrin gel. There are also no particular limitations on the concentrations and volume ratios of the respective solutions L1 and L2, the amount applied per unit time, the application pressure, etc. The specific forms of the respective solutions L1 and L2 used in this embodiment can be determined by referring to known information (e.g., JP 2014-179151 A, etc.).

[0036] The control unit 120 is configured to comprehensively control each unit of the shaping system 100. The control unit 120 is configured with a CPU (Central Processing Unit), memories such as RAM (Random Access Memory) and ROM (Read Only Memory), and a storage unit, and controls each of the above units and performs various arithmetic processing according to a program.

[0037] The storage unit is configured with an HDD (Hard Disk Drive) or an SSD (Solid State Drive) and stores various programs and data. The storage unit can store a series of programs required for controlling the operation of the shaping system 100. The above programs can incorporate algorithms for causing the control unit 120 to function as a detection unit 121, a determination unit 122, and a calculation unit 123.

[0038] The operational control executed by the control unit 120 includes the operation of shaping the reinforcement layer 30 using the shaping system 100. In carrying out the above operation, the control unit 120 controls the operation of the shaping system 100 to execute the following steps: a step in which the detection unit 121 detects the thickness of the reinforcement layer 30; a step in which the calculation unit 123 calculates the height of the resection unit 130 when the resection unit 130 resects the reinforcement layer 30 in the region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value; and a step in which the resection unit 130 moves horizontally from the height calculated by the calculation unit 123 to resect the reinforcement layer 30 in the region 30H where the thickness of the reinforcement layer 30 is equal to or greater than the predetermined value. Specific steps of the shaping method will be described later. The predetermined value of the thickness of the reinforcement layer 30 is not particularly limited, but is 1000 to 5000 μm.

[0039] The detection unit 121 is configured to detect the thickness of the reinforcing layer 30 based on the image information acquired by the imaging unit 140. Specifically, the detection unit 121 detects the thickness of the reinforcing layer 30 based on the image information of the sheet-shaped cell culture 10 and the image information of the laminate 20 acquired by the imaging unit 140. For this reason, as shown in FIG. 1 , the imaging unit 140 is disposed on the front side of the sheet-shaped cell culture 10, and images are taken along the horizontal direction. In this case, it is also possible to capture images of the 360° periphery of the sheet-shaped cell culture 10. The detection unit 121 detects a region 30H in the reinforcing layer 30 where the thickness is equal to or greater than a predetermined value. A plurality of regions 30H may exist in the laminate 20 consisting of the sheet-shaped cell culture 10 and the reinforcing layer 30.

[0040] Based on the thickness data of the reinforcing layer 30 detected by the detection unit 121, the determination unit 122 determines the region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value.

[0041] The calculation unit 123 is programmed to calculate the height and horizontal position of the cutting unit 130 when cutting out unnecessary reinforcement layer 30 in region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value by the cutting unit 130. Specifically, the calculation unit 123 stores in advance the height of the cutting unit 130 when cutting out unnecessary reinforcement layer 30 in region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value by the cutting unit 130.

[0042] Here, the height of the excision portion 130 may be the sum (see dotted lines in FIGS. 3 and 4) of the maximum thickness of the sheet-shaped cell culture 10 and a predetermined value of the thickness of the reinforcing layer (see two-dot chain lines in FIGS. 3 and 4). The height of the excision portion 130 may be the average or median value of the thickness of the reinforcing layer within a predetermined range (to match the horizontal, smooth portion of the already formed reinforcing layer 30).

[0043] The control unit 120 controls the operation of the cutting unit 130 to move the cutting unit 130 horizontally from the height calculated by the calculation unit 123. In this way, the cutting unit 130 cuts off unnecessary reinforcement layer 30 in the region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value. The cutting by the cutting unit 130 targets the entire top surface of the laminate 20 (from one end to the other end).

[0044] The cutting portion 130 can be configured to include a mechanism such as a cutting tool (for example, a knife, a cutter, or a scalpel) that can cut the reinforcing layer 30. Note that the shape of the cutting tool is not limited as long as it can cut in the horizontal direction, and the cutting tool may be deployed in a fan shape from the center position of the laminate 20 to perform the cutting.

[0045] The imaging unit 140 can be configured with a known camera device capable of acquiring still image data and / or video data. As shown in Fig. 1, the imaging unit 140 is disposed on the front side of the sheet-shaped cell culture 10 in order to detect the thickness of the sheet-shaped cell culture 10 and the thickness of the laminate 20.

[0046] In the shaping system 100 according to this embodiment, the detection unit 121 detects the thickness of the laminate 20 based on image information acquired by the imaging unit 140. However, the detection unit 121 may also detect the thickness of the laminate 20 based on the light transmittance of the inspection light f irradiated by the light irradiator 160. The configuration of the light irradiator 160 will be described below.

[0047] As shown in FIG. 1, the light irradiating unit 160 can be configured with a light source (e.g., a known lamp) that irradiates the laminate 20 contained in the container 220 with inspection light f of a predetermined wavelength. There are no particular limitations on the inspection light f irradiated by the light irradiating unit 160. Measurement of light transmittance may be performed using a transmission method in which a light source is provided on the back surface of the sheet-shaped cell culture 10 as viewed from the light receiver, or a reflection method in which an illumination light source is provided on the light receiver side and a reflective surface is provided on the back surface of the sheet-shaped cell culture 10. Furthermore, in both transmission and reflection methods, ambient light may be used as the light source without providing a special light source, and the intensity of light transmitted through the sheet-shaped cell culture 10 may be measured. When targeting the sheet-shaped cell culture 10, visible light is typically used; however, any radiation whose transmittance varies depending on the thickness of the sheet-shaped cell culture 10 can also be used. A transmittance wavelength of 400 to 800 nanometers is optimal for detection sensitivity.

[0048] As shown in Fig. 1, the light irradiation unit 160 can be laid out above a stage 210 on which a container 220 containing a sheet-shaped cell culture 10 is placed. It is also possible to irradiate the sheet-shaped cell culture 10 with the inspection light f from below and detect the light transmittance on the upper surface of the sheet-shaped cell culture 10. As shown in Fig. 3, the light irradiation unit 160 can be laid out so as to irradiate the inspection light f over the entire range in the planar direction of the sheet-shaped cell culture 10 constituting the laminate 20 (the entire range of the surface 10a).

[0049] The detection unit 121 is configured to be able to detect the thickness of the laminate 20 based on the light transmittance of the inspection light f irradiated onto the laminate 20. The detection unit 121 irradiates each portion of the laminate 20 with the inspection light f when the fibrin gel has sufficiently solidified, and detects the light transmittance of each portion of the laminate 20. The detection unit 121 can detect the thickness by converting the light transmittance of each portion of the laminate 20 into the thickness of the laminate 20, taking into consideration pre-stored reference data (data relating to the relationship between light transmittance and thickness). In this case, the calculation unit 123 calculates the thickness of the reinforcing layer 30 by subtracting the thickness of the sheet-shaped cell culture 10 detected based on the image information of the sheet-shaped cell culture 10 acquired by the imaging unit 140 from the thickness of the laminate 20 detected based on the light transmittance of the inspection light f.

[0050] The image display unit 150 can be configured, for example, by a known liquid crystal display or the like.

[0051] The orthodontic system 100 may also be configured as a portable or non-portable information terminal device in which the control unit 120, the imaging unit 140, and the image display unit 150 are integrally incorporated.

[0052] <Formatting method> Next, a shaping method according to this embodiment will be described.

[0053] The specific work content of each step will be described below with reference to the flowchart shown in Fig. 2 and Fig. 3 to Fig. 5. The following description also explains an example in which the shaping system 100 shown in Fig. 1 automatically performs steps S10 to S16 of the shaping method.

[0054] To start the shaping method, a container 220 is prepared, which contains a sheet-shaped cell culture 10 before the formation of the reinforcing layer 30 made of fibrin gel. The container 220 is placed on a stage 210 (see FIG. 1).

[0055] Next, the detection unit 121 detects the thickness of the sheet-shaped cell culture 10 (step S10). As a method for detecting the thickness of the sheet-shaped cell culture 10, detection is performed based on image information captured by the imaging unit 140, as described above.

[0056] Next, the fibrinogen solution L1 is supplied (applied) to the sheet-shaped cell culture 10 by the first applicator 111 of the processing unit 110 (step S11).

[0057] Next, the second applicator 112 of the processing unit 110 supplies (applies) the thrombin solution L2 to the sheet-shaped cell culture 10 (step S12). When the thrombin solution L2 is supplied to the sheet-shaped cell culture 10, the fibrinogen solution L1 and the thrombin solution L2 applied to the sheet-shaped cell culture 10 start to react, and solidification proceeds accordingly.

[0058] Next, the detection unit 121 detects the thickness of the laminate 20 (step S13). As described above, the method for detecting the thickness of the laminate 20 may be based on image information captured by the imaging unit 140, or may be based on the light transmittance of the inspection light f irradiated by the light irradiating unit 160.

[0059] Next, the calculation unit 123 calculates the thickness of the reinforcing layer 30 based on the thickness of the sheet-shaped cell culture 10 detected in step S10 and the thickness of the laminate 20 detected in step S13 (step S14). Specifically, the calculation unit 123 calculates the thickness of the reinforcing layer 30 by subtracting the thickness of the sheet-shaped cell culture 10 detected in step S10 from the thickness of the laminate 20 detected in step S13.

[0060] Next, the detection unit 121 detects regions 30H in the reinforcing layer 30 where the thickness is equal to or greater than a predetermined value (step S15). If the detection unit 121 does not detect regions 30H in the reinforcing layer 30 where the thickness is equal to or greater than a predetermined value (step S15: NO), the shaping method ends.

[0061] On the other hand, as shown in FIG. 3 , if the detection unit 121 detects a region 30H in which the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value in step S15 (step S15: YES), the control unit 120, as shown in FIGS. 4 and 5 , positions the resection unit 130 at the height and horizontal position calculated by the calculation unit 123, and moves the resection unit 130 along the calculated position in the horizontal direction to remove unnecessary reinforcement layer 30 in the region 30H in which the thickness of the reinforcement layer 30 is equal to or greater than the predetermined value (step S16). Note that the control unit 120 may position the resection unit 130 at the height calculated by the calculation unit 123 (regardless of the position) and move the resection unit 130 over a wide range in the horizontal direction to remove unnecessary reinforcement layer 30 in the region 30H in which the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value. Here, moving the resection unit 130 along the calculated position in the horizontal direction can shorten the moving distance and the resection time. The process of step S16 can prevent the reinforcement layer 30 from being formed at an extremely thick location in the laminate 20. This makes it possible to increase the strength of each portion of the laminate 20 uniformly.

[0062] Next, the process returns to step S13, and step S16 is repeated until it is determined that the reinforcing layer 30 does not need to be cut off (step S15: NO).

[0063] As described above, the orthopedic system 100 of this embodiment includes a detection unit 121 that detects the thickness of the reinforcing layer 30 made of fibrin gel formed on the sheet-shaped cell culture 10, a resection unit 130 that excises the reinforcing layer 30, a calculation unit 123 that calculates the height of the resection unit 130 when the resection unit 130 excises the reinforcing layer 30 in region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value, and a control unit 120 that controls the resection unit 130 to move horizontally from the height calculated by the calculation unit 123, thereby excising the reinforcing layer 30 in region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value.

[0064] In addition, the shaping method of this embodiment includes the steps of: a step in which the detection unit 121 detects the thickness of the reinforcing layer 30 formed on the sheet-shaped cell culture and consisting of fibrin gel; a step in which the calculation unit 123 calculates the height of the excision unit 130 when the reinforcing layer 30 in the region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value is excised by the excision unit 130; and a step in which the excision unit 130 moves horizontally from the height calculated by the calculation unit 123 to excise the reinforcing layer 30 in the region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value.

[0065] In addition, the program for executing the shaping method of this embodiment is a program that causes a system having an excision section 130 that excises the reinforcement layer 30 made of fibrin gel in region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value to execute the following steps: detecting the thickness of the reinforcement layer 30; calculating the height of the excision section 130 when the reinforcement layer 30 in region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value is excised by the excision section 130; and controlling the excision section 130 so as to excise the reinforcement layer 30 in region 30H where the thickness of the reinforcement layer 30 is equal to or greater than a predetermined value at the calculated height.

[0066] According to this embodiment, the detection unit 121 detects the thickness of the reinforcing layer 30, the calculation unit 123 calculates the height of the reinforcing layer 30 when the reinforcing layer 30 is used to remove the reinforcing layer 30 in the region 30H where the thickness of the reinforcing layer 30 is equal to or greater than a predetermined value, and the reinforcing layer 30 moves horizontally from the height calculated by the calculation unit 123 to remove the reinforcing layer 30 in the region 30H where the thickness of the reinforcing layer 30 is equal to or greater than the predetermined value. This eliminates the region 30H where the thickness of the reinforcing layer 30 is equal to or greater than the predetermined value, thereby enabling the laminate 20 to be formed uniformly. Note that "formed uniformly" here does not necessarily mean that the thickness is completely constant.

[0067] Although the system for forming the reinforcing layer 30, the method for forming the reinforcing layer 30, and the program according to the present invention have been described through the embodiments, the present invention is not limited to the configurations described in the embodiments, and can be modified as appropriate based on the claims. [Explanation of symbols]

[0068] 10. Sheet-shaped cell culture 20 laminate 30 Reinforcement layer 30H Region where the thickness of the reinforcing layer is equal to or greater than a specified value 100 Orthopedic System 110 Processing section 111 First application section 112 Second application section 120 control section 121 Detector 122 Judgment section 123 Calculation Unit 130 Excision part 140 Imaging unit 150 Image display unit 160 Light irradiation unit 210 Stages 220 Container L1 fibrinogen liquid L2 thrombin solution f Inspection light

Claims

1. a detection unit for detecting the thickness of a reinforcing layer made of fibrin gel formed on the sheet-shaped cell culture; a cutting portion for cutting the reinforcing layer; a calculation unit that calculates a height of the cutting portion when cutting the reinforcing layer in a region where the thickness of the reinforcing layer is equal to or greater than a predetermined value; A system for shaping a reinforcement layer, comprising: a control unit that controls the cutting unit to move horizontally from the height calculated by the calculation unit, thereby cutting out the reinforcement layer in areas where the thickness of the reinforcement layer is greater than or equal to a predetermined value.

2. an imaging unit for imaging the sheet-shaped cell culture and the reinforcing layer, The system for shaping a reinforcing layer according to claim 1 , wherein the detection unit detects the thickness of the reinforcing layer based on image information acquired by the imaging unit.

3. a light irradiation unit that irradiates the sheet-shaped cell culture and the reinforcing layer with an inspection light; The system for shaping a reinforcing layer according to claim 1 or 2, wherein the detection unit detects the thickness of the reinforcing layer based on the light transmittance of the inspection light through the sheet-shaped cell culture and the reinforcing layer.

4. The system for shaping a reinforcing layer according to claim 1 or 2, wherein the calculation unit also calculates a horizontal position when the cutting unit cuts the reinforcing layer.

5. The system for shaping a reinforcing layer according to claim 1 or 2, further comprising a processing unit for forming the reinforcing layer on the sheet-shaped cell culture.

6. a step in which a detection unit detects the thickness of a reinforcing layer made of fibrin gel formed on the sheet-shaped cell culture; a step in which a calculation unit calculates a height of a cutting portion when cutting the reinforcing layer in a region where the thickness of the reinforcing layer is equal to or greater than a predetermined value; A method for shaping a reinforcement layer, comprising a step in which the cutting unit moves horizontally from the height calculated by the calculation unit to cut out the reinforcement layer in areas where the thickness of the reinforcement layer is greater than or equal to a predetermined value.

7. A system having a resection unit that resects a region of a reinforcing layer made of fibrin gel where the thickness of the reinforcing layer is equal to or greater than a predetermined value, detecting a thickness of the reinforcing layer; calculating a height of the cutout portion when cutting out the reinforcing layer in a region where the thickness of the reinforcing layer is equal to or greater than a predetermined value; and controlling the cutting unit to cut off the reinforcing layer at a calculated height in an area where the thickness of the reinforcing layer is equal to or greater than a predetermined value.

Images