Plant heat resistance or salt resistance improver

Abstract

The present invention provides an agent and method for improving the heat resistance or salt tolerance of plants. [Solution] A plant heat resistance or salt resistance improving agent is provided, which contains acetic acid or a salt thereof, or a solvate thereof.

Description

Technical Field

[0003] , , , , , ,

[0001] The present invention relates to an agent for improving the heat resistance or salt tolerance of plants.

Background Art

[0002] Currently, due to the explosive increase in population, producing sufficient amounts of food plants has become a global issue. In addition, desertification of land due to the reduction of green spaces has also become a problem. Moreover, the rise in surface temperature based on the global warming phenomenon has also become a problem. Therefore, the environmental problems related to plant growth are increasing. That is, excessive stress based on global environmental factors in plants often causes problems in plant growth. One of the stresses on plants is drought stress. Regarding drought stress, attempts have been made to create genetically modified plants with modified drought stress-responsive genes and to apply chemical or biological regulators that improve drought stress tolerance. Patent Document 1 discloses a method for improving the drought stress tolerance of plants, including a step of applying 10 mM or more of acetic acid to the roots of plants by perfusion and growing the plants under drought stress conditions.

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Prior Art Documents

Patent Documents

[0005] [Patent Document 1] U.S. Patent No. 9,258,954 [Non-patent literature]

[0006] [Non-Patent Document 1] Kim, JM et al., Nature Plants, Vol.3, 17097 (2017) [Overview of the Initiative] [Problems that the invention aims to solve]

[0007] In plant growth, stresses other than drought also exist as stressors on plants. To exist. Therefore, to counteract the stress on plant growth in high-temperature environments and saline soils, The study focused on whether it was possible to impart resistance to materials. The problem that this invention aims to solve is to impart heat resistance or salt resistance to plants. The objective is to provide a means to achieve this. [Means for solving the problem]

[0008] The inventors of the present invention have conducted diligent studies to solve the above problem and have found that acetic acid and the like are subjected to thermal stress. We have found that this contributes to the ability of plants to grow well in response to salt stress, and this invention I completed it.

[0009] In other words, the present invention is as follows. (1) A plant containing acetic acid or its salts, or solvates thereof, for heat resistance or salt resistance. An additive. (2) (1) further contains one or more solvents including at least water, which is heat-resistant or Salt resistance improver. (3) The heat resistance or salt tolerance improver according to (2), wherein the pH ranges from 3 to 9. (4) The heat resistance or salt tolerance improver according to (2) or (3), which contains acetic acid or its salt in the range of 0.01 to 0.5% by volume. (5) A composition for improving the heat resistance or salt tolerance of plants, which contains acetic acid or its salt, or their solvates. (6) The composition according to (5), which further contains one or more solvents including at least water. (7) The composition according to (6), wherein the pH ranges from 3 to 9. (8) The composition according to claim 6 or 7, which contains acetic acid or its salt in the range of 0.01 to 0.5% by volume. (9) A method for improving the heat resistance or salt tolerance of plants, which includes applying acetic acid or its salt, or their solvates to plants, materials for applying to plants, or soil, medium or culture solution in which plants grow. (10) Obtaining one or more pieces of information related to plant growth, Based on the one or more pieces of information obtained, determining the conditions for applying the heat resistance or salt tolerance improver according to any one of (1) to (4) or the composition according to any one of (5) to (8) to plants, materials for applying to plants, or soil, medium or culture solution in which plants grow. , A method for managing plant growth, which includes the above.

Advantages of the Invention

[0010] According to the present invention, it becomes possible to provide means for imparting heat resistance or salt tolerance to plants.

Brief Description of the Drawings

[0011] [Figure 1] Figure 1 shows the results of heat resistance test 1 using lettuce (Citrus sempervirens) in the example. The left side shows the symmetrical water-supplied group, and the right side shows the 0.1 vol% acetic acid aqueous solution-supplied group. [Figure 2] Figure 2 shows the results of heat resistance test 2 using lettuce in the example. In both Figure 2A and Figure 2B, the left side shows the symmetrical water-supplied group, and the right side shows the 0.06 vol% acetic acid aqueous solution-supplied group. Figure 2A shows lettuce before growth under a temperature of 42°C, and Figure 2B shows lettuce after growth under a temperature of 42°C. Figure 2C shows a graph of survival rates. [Figure 3] Figure 3 shows the results of heat resistance test 3 using tomatoes in the example. In both Figure 3A and Figure 3B, the left side shows the symmetrical water-supplied group, and the right side shows the 0.06 vol% acetic acid aqueous solution-supplied group. Figure 3A shows tomatoes before growth under a temperature of 42°C, and Figure 3B shows tomatoes after growth under a temperature of 42°C. [Figure 4] Figure 4 shows the results of the salt resistance test using lettuce in the example. The left side shows the symmetrical water-supplied group, the middle side shows the 0.1 vol% acetic acid aqueous solution-supplied group, and the right side shows the 0.2 vol% acetic acid aqueous solution-supplied group. [Modes for carrying out the invention]

[0012] The embodiments of the present invention will be described in detail below, but the present invention is not limited to the following embodiments. It is not something that is fixed, but can be implemented with various modifications.

[0013] The present invention relates to acetic acid or its salts, or solvates thereof (hereinafter referred to herein as " It is a plant heat-resistant or salt-resistant agent containing acetic acid, etc. (sometimes referred to as "acetic acid, etc."). In this invention, by applying a plant heat resistance or salt resistance improving agent to plants, The heat resistance and / or salt resistance of plants can be improved. The salt resistance improver may also be a heat resistance improver, and the salt resistance improver may also be a heat resistance improver It may also be a preservative and a salt tolerance improver. The plant heat resistance or salt resistance improving agent of the present invention can be used as a pesticide or agricultural chemical formulation. can. In this invention, by applying a plant heat resistance or salt resistance improving agent to plants, Under conditions such as high temperatures and saline soils, which have undesirable effects on plant growth This can confer resistance to plant stress during plant growth. To confer tolerance to plant stress during plant growth does not mean complete tolerance. It doesn't have a taste.

[0014] In the present invention, the plant heat resistance or salt resistance improver includes acetic acid, etc. Here, acetic acid, etc., exhibits its properties as an enhancer of heat resistance or salt tolerance in plants, Plant heat tolerance or salt tolerance improvers include, but are not limited to, acetic acid as an active ingredient. It contains. The active ingredient is acetic acid, which exhibits its properties as a heat-resistant or salt-resistant agent for plants. This means that it is an ingredient. The acetic acid used in this invention may be acetic acid for industrial use, or vinegar for food use. Acids can also be used. Acetic acid includes wood vinegar, which is used in agriculture, and brewed vinegar, which is produced through fermentation. Such safe and inexpensive acetic acid may be used. Using these acetic acids, etc., as heat-resistant or salt-resistant agents for plants in the present invention is possible. It is suitable because it is highly safe and low-cost.

[0015] The acetic acid used in this invention may be acetic acid itself, or a salt of acetic acid. stomach. The salt of acetate is not particularly limited as long as it is a salt that can supply acetate ions. Examples include salts with cations, specifically sodium acetate, potassium acetate, and calcium acetate. Examples include ammonium, magnesium acetate, zinc acetate ions, and ammonium acetate. In ammonium acetate, ammonium ions are replaced with substituted or unsubstituted ammonium ions. It may also be an acetate with ammonium. Among them, the salts of acetic acid include potassium acetate, sodium acetate, ammonium acetate, and acetic acid. Magnesium and calcium acetate can be suitably used. Also, eggshells can be dissolved in vinegar. The resulting liquid fertilizer can also be used as acetic acid or similar substances. In the present invention, acetic acid and acetate may be used as a mixture. For example, it may be a mixture of acetic acid itself and an acetate salt, and acetic acid with acetate ions and By adding a cation source that forms a salt, for example, vinegar is produced by a neutralization reaction as an aqueous solution. It may also be a mixture of acid and acetate. In this case, the acetic acid may be completely neutralized, or vinegar. A cation source may be added in an amount that partially neutralizes the acid. Examples of aqueous solutions containing acetate include acetate buffer solutions.

[0016] The acetic acid used in this invention may be a solvate of acetic acid or a solvate of an acetate salt. stomach. The solvents that can form solvates of acetic acid or its salts are not particularly limited, however For example, water, as well as alcohol, dimethyl sulfoxide (DMSO), ethanolamine Examples include organic solvents such as ethyl acetate. The alcohol can be, for example, a lower alcohol, or a higher alcohol. It is also acceptable. The lower alcohols are not particularly limited, but for example, methanol A substance with 1 or more carbon atoms, such as ethanol or 2-propanol (isopropyl alcohol). Examples include saturated or unsaturated linear or branched alkyl alcohols, etc. The term is not particularly limited, but for example, 1-heptanol or 1-octa Saturated or unsaturated linear or branched alkyl alcohols with 7 or more carbon atoms, such as nool These are some examples. The solvent that forms the solvate may be a single solvent or two or more solvents. stomach. Regarding solvents, for example, when used as an aqueous solution of a solvate, acetic acid, etc. in the aqueous solution. The form is not particularly limited, and it does not need to be solvated.

[0017] In this invention, "heat resistance improver" and "improves heat resistance" refer to the present invention in relation to a group of plants. By applying a heat-resistant agent to plants, it becomes possible to prevent plants from becoming unable to grow (dying) or growing poorly (for example, plant Whitening or yellowing of the whole or part of an object (for example, leaves or flowers), reduction in root length, or (This may include a decrease in the number of leaves or lodging), a decrease in growth rate, or a decrease in plant weight or crop yield. This substantially reduces heat stress, which is an undesirable effect on plant growth. It means that it is possible. Confirmation that it is a "heat resistance improver" and that it "improves heat resistance" is necessary for the plant resistance of the present invention. This can be confirmed by comparing it with a control population of plants that were not treated with a heat enhancer, and also, usually 30% or more, preferably 50% or more, 60% or more, 70% or more, more preferably 80% or more Furthermore, more preferably, the survival rate is improved by 85% or more, and particularly preferably by 90% or more. This can be confirmed by [this method].

[0018] In this specification, "thermal stress" means being placed in an environment with a temperature of 60 degrees Celsius or lower. The temperature may be 50°C or lower, or 45°C or lower. Furthermore, considering that a constant temperature is usually around 25°C, it is preferable to be in an environment of 30°C or higher. It is more preferable that the environment be at 35°C or higher. In this specification, "thermal stress" refers to being placed in an environment with a temperature range of 30 to 60°C. This may be done, and upper and lower limits may be set within the said temperature range. In this invention, confirmation that it is a "heat resistance improver" or that it "improves heat resistance" is necessary. While not particularly limited, evaluation may also be carried out by the following means, and more specifically, The evaluation may also be conducted using the methods described in the examples. For example, the target plant is subjected to a certain amount of test solution (water and, if applicable, normal nutrients). (m) Grow under normal growth conditions, i.e., under non-heat stress conditions. Grow for a certain period of time. After that, a heat-resistant agent was applied, and then the plants were grown under heat stress conditions, followed by normal conditions. The plants were grown under normal growing conditions, i.e., non-heat stress conditions, and their survival rate was measured. It can be evaluated by doing so. The thermal stress conditions include leaving the sample undisturbed for 30 minutes or more under the temperature conditions described above as "thermal stress". If that is the case, the standing time can be set according to the temperature conditions. Under thermal stress conditions, the conditions are constant humidity and, in particular, conditions in which the system is left undisturbed without water supply. This is preferable.

[0019] In this invention, "salt tolerance improver" and "improves salt tolerance" refer to the present invention in relation to a group of plants. By applying a salt tolerance enhancer to plants, it becomes possible to prevent inability to grow (death) or poor growth (for example, plant Whitening or yellowing of the whole or part of an object (for example, leaves or flowers), reduction in root length, or (This may include a decrease in the number of leaves or lodging), a decrease in growth rate, or a decrease in plant weight or crop yield. Salt stress, specifically high concentrations, is an undesirable effect on plant growth. This means that salt stress can be substantially reduced. Confirmation that it is a "salt tolerance enhancer" and that it "improves salt tolerance" is necessary for the plants of this invention. This can be confirmed by comparing it to a control population of plants that were not treated with a hydrochloric acid enhancer, and is usually 30% or more, preferably 50% or more, 60% or more, 70% or more, more preferably 80% or more Furthermore, more preferably, the survival rate is improved by 85% or more, and particularly preferably by 90% or more. This can be confirmed by [this method].

[0020] In this specification, "salt stress" means being exposed to an environment with high concentrations of salts. While there are no specific limitations regarding the concentration of salts, soils classified as saline soils are... This may be the salt concentration in the soil. Salts that cause salt stress are not limited to phosphates, nitrates, etc. Salts such as salt salts and hydrochloride salts, which are found in saline soils, may be included. Salts include, specifically, sodium chloride, magnesium chloride, phosphoric acid, nitrates, and salts. Examples include alkali metal salts of acids, alkali metal salts, and ammonium salts. In this invention, confirmation that it is a "salt resistance improver" or that it "improves salt resistance" is necessary. While not particularly limited, evaluation may also be carried out by the following means, and more specifically, The evaluation may also be conducted using the methods described in the examples. For example, the target plant is subjected to a certain amount of test solution (water and, if applicable, normal nutrients). (m) Grow under normal growth conditions, i.e., non-salt stress conditions. Grow for a certain period of time. After that, a salt tolerance enhancer was applied, and then the plants were grown under salt stress conditions, and then under normal conditions. The plants were grown under specific growth conditions, i.e., non-salt stress conditions, and their survival rate was measured. It can be evaluated by doing so. The salt stress conditions involve leaving the samples undisturbed for 30 minutes or more under the temperature conditions described above for "salt stress". If that is the case, the standing time may be set according to the type and concentration conditions of the salts. Under salt stress conditions, even under conditions of constant humidity and continuous water supply, Often, it is acceptable to leave the plants undisturbed without supplying water.

[0021] In the present invention, the plant heat resistance or salt resistance improver is a plant heat resistance improver. It can also be a plant salt tolerance enhancer, and a plant heat tolerance enhancer, as well as a plant It may be a salt resistance improver. In the present invention, the heat resistance or salt resistance improver for plants is a composition containing acetic acid, etc. It may also be a composition that improves the heat resistance or salt resistance of plants. In other words, in this specification, "plant heat resistance or salt resistance improver" means "plant It can also be understood to have the same meaning as "a composition that improves heat resistance or salt resistance."

[0022] In each embodiment of the present invention, the effect of the plant heat resistance or salt resistance improving agent is that the plant's growth is controlled Effects on the body, for example, elongation of stems, leaves, or roots, increase in the number of leaves, flowering, or fruiting. Promotion, increase in the number of flowers or fruits, increase in plant weight or crop yield, greening, or tillering It may also include growth-promoting effects such as the acceleration of growth.

[0023] In this invention, by applying acetic acid or the like to plants, heat stress or salt stress can be reduced. This can improve heat resistance or salt resistance in relation to the material. The present invention relates to an agent for improving the heat resistance or salt resistance of plants, and an agent for improving the heat resistance or salt resistance of plants. The above composition is used as an agricultural chemical preparation or pesticide to promote plant growth. It is possible.

[0024] In the present invention, the heat resistance or salt resistance improving agent for plants is, for example, a solid (for example, a powder) In any form such as granular material, liquid (e.g., solution or suspension), or gas. That's fine. In the present invention, the heat resistance or salt resistance improving agent for plants is a liquid such as a solution or suspension. It is preferable to use it in this form. In the present invention, when a plant heat resistance or salt resistance improving agent is used as a solution, the liquid It may be used in its current state, or as a liquid prepared on the spot when applying it.

[0025] In the plant heat resistance or salt resistance improving agent of the present invention, acetic acid, etc., preferably as an active ingredient It may be used alone, or in combination with one or more agriculturally acceptable ingredients. That's good too. The plant heat resistance or salt resistance improving agent of the present invention, as an agrochemical or agricultural chemical preparation, is desired Depending on the method of application, it can be formulated into various dosage forms commonly used in the relevant technical field. ru. The plant heat resistance or salt resistance improving agent of the present invention, in addition to acetic acid, contains one or more agriculturally permissible substances. It may further contain the ingredients that are included. Agriculturally permissible components include solvents or carriers, excipients, binders, solubilizers, and ammonium compounds. Stabilizers, thickeners, leavening agents, lubricants, surfactants, oily liquids, buffers, disinfectants, antifreezes, defoamers Examples include colorants, antioxidants, additives, fertilizers, and further chemicals. Agriculturally acceptable solvents or carriers include water, kerosene, or diesel oil. Eel mineral oil fraction, vegetable or animal-derived oils, cyclic or aromatic hydrocarbons (e.g., paraffin fin, tetrahydronaphthalene, alkylated naphthalenes or their derivatives, Alkylated benzenes or their derivatives), alcohols (e.g., methanol, ethanol) Butanol, propanol, butanol, ethylene glycol, glycerol or cyclohexyl Sanol), ketones (e.g., cyclohexanone), or amines (e.g., N-methyl methyl amines). Agriculturally acceptable solvents or liquid carriers such as rolidone, or mixtures thereof, are preferred. More preferably, one or more solvents containing at least water are used. As fertilizer, organic fertilizers such as oil cake or cow manure, or ammonium sulfate, calcium cyanamide or fused Inorganic fertilizers such as phosphorus are preferred.

[0026] The plant heat resistance or salt resistance improving agent of the present invention comprises at least one solvent including water. Furthermore, if present, the pH of the solution of the plant heat resistance or salt resistance improver of the present invention is preferred. Or it is in the range of 3 to 9, and within that range the lower limit of pH is 4 or higher, 5 or higher, and 6 or higher. It is also fine to have upper limits of pH of 8.5 or less, 8 or less, 7.5 or less, or 7 or less. . The pH range is more preferably in the range of 4 to 8, and even more preferably in the range of 5 to 7.5. The pH range is within the range of 5 to 7. You can also use 6 or 7. The pH of plant heat tolerance or salt tolerance improvers should be within the aforementioned range at the time of application to the target plant. It is preferable to have one. The pH of the plant heat resistance or salt resistance improving agent of the present invention may be adjusted with an acid or alkali. For example, hydrochloric acid, nitric acid, sulfuric acid, sodium hydroxide, potassium hydroxide, ammonia water, or vinegar. It may also be prepared using an acid, alkali, or buffer, such as ammonium ammonium acid. In the plant heat resistance or salt resistance improving agent of the present invention, the pH is adjusted to the target plant. It is preferable that the value is within the above range at the time of application, but if, for example, the value is within the above range at the time of application to the target plant, Even if it is not within the specified range, the pH buffering effect of the soil, culture medium, or culture solution to be applied can be utilized. The pH may be adjusted to a range.

[0027] The plant heat resistance or salt resistance improving agent of the present invention comprises at least one solvent including water. Furthermore, if it is contained, the total amount of acetic acid, etc. in the heat resistance or salt resistance improver for plants of the present invention The ratio to the product is preferably in the range of 0.01 to 0.5 volume%, and within that range the ratio The lower limits are 0.05 volume% or more, 0.075 volume% or more, 0.09 volume% or more, and 0.1 It may be 0% by volume or more, and the upper limit of the percentage is 0.25% by volume or less, and 0.2% by volume or less. That's fine. The range of the proportion of acetic acid, etc., is more preferably in the range of 0.05 to 0.5 volume%, and further Preferably in the range of 0.075 to 0.25 volume%, and more preferably 0.09 to The range is 0.2% by volume, and particularly preferably in the range of 0.1 to 0.2% by volume.

[0028] The plant heat resistance or salt resistance improving agent of the present invention comprises at least one solvent including water. Furthermore, if present, the concentration of acetic acid, etc., in the heat resistance or salt resistance improver for plants of the present invention. The concentration is preferably in the range of 1 to 100 mM, and within that range, the lower limit of the concentration is 2 mM or higher. The upper limit of the concentration may be 5 mM or higher, 7.5 mM or higher, 9 mM or higher, or 10 mM or higher. The concentration may be 50 mM or less, or 40 mM or less. The range of the proportion of acetic acid, etc., is more preferably in the range of 1 to 50 mM, and even more preferably The range is 7.5 to 50 mM, more preferably 9 to 40 mM, and especially Preferably, the concentration is in the range of 10 to 40 mM.

[0029] The plant heat resistance or salt resistance improving agent of the present invention comprises at least one solvent including water. Furthermore, if present, the pH is within the aforementioned range, and the concentration of acetic acid, etc., is within the aforementioned range. It is preferable to do so. pH and concentration can be appropriately selected together or independently within the aforementioned range. ru.

[0030] The plant heat resistance or salt resistance improving agent of the present invention contains one or more further agents. That's fine. The drugs in question are not limited to those mentioned above, but examples include auxin and gibberellin. Cytokinin, 2-chloroethylphosphonic acid (product name: Esrel (registered trademark)), -Baid, benzyladenine, brassinosteroid, strigolactone and jasmonic acid Examples include the above. Furthermore, the drugs in question are plant hormones commonly used in this technical field. , plant chemical regulators and pesticides may also be used.

[0031] In the present invention, the target plants are not particularly limited, and for example, angiosperms. And selected from gymnosperms. The target plants are not particularly limited, but for example, chrysanthemums and gerberas. Plants of the Asteraceae family such as potatoes, tomatoes and eggplants, rapeseed and flies Brassicaceae plants such as rana, rice, corn, wheat, sugarcane and barley Grasses like soybeans, legumes like soybeans, morning glories like morning glories, pop Salicaceae plants like ra, castor bean, cassava, and spurge like jatropha Plants of the Convolvulaceae family, such as sweet potatoes, and plants of the Rutaceae family, such as oranges and lemons. , plants of the Rosaceae family such as cherry blossoms and roses, plants of the Orchidaceae family such as Phalaenopsis orchids, and lisianthus Gentian-like plants, cyclamen-like primrose-like plants, pansies-like plants Millet plants, lily-like lily-family plants, sugar beet-like Amaranth-family plants, grape-like bushes Plants of the Viburnum family, plants of the Cupressaceae family such as Japanese cedar and cypress, and trees such as olive and osmanthus. Examples include plants of the Salicaceae family, as well as plants of the Pinaceae family, such as Japanese red pine. The plants targeted by the plant heat resistance or salt resistance improver of the present invention are those containing acetic acid, including acetic acid synthesis. They share common metabolic mechanisms. Therefore, these are conserved in plants regardless of species. Through the action of a group of functional genes, heat tolerance or salt tolerance to acetic acid, etc., is commonly exhibited in plants. It is considered possible. The plants considered include not only the whole plant (i.e., a complete plant body), but also a group of plants. Tissue or organ (for example, cut flowers, or vegetative propagation such as rhizomes, tubers, corms, or runners) This may include organs, cultured cells, and / or parts of plants such as callus. The plant heat resistance or salt resistance improving agent of the present invention is applicable to any plant, including before or after germination. The whole or part of a plant in a growth stage (for example, a seed, seedling or the whole or part of a mature plant) It can be applied to the part shown.

[0032] The plant heat resistance or salt resistance improving agent of the present invention is not only for the plant itself, but also when applied to the plant. It may be used as a material for that purpose, or applied to the soil, growing medium, or culture solution in which plants grow. The present invention relates to the application of acetic acid, preferably in an agriculturally effective amount, to plants, and to the application of acetic acid, etc. This includes applying it to materials for plant growth, or to the soil, growing medium, or culture solution in which plants grow. This also relates to methods for improving heat resistance or salt resistance. In the method of the present invention, the acetic acid and other substances applied are determined by the heat resistance of the plant or As described herein, it is used as a salt resistance improver. The materials used for plant application are not particularly limited, but examples include water and fertilizer. Examples include various materials commonly used in the relevant technical field, such as fertilizers.

[0033] The dosage form of the plant heat resistance or salt resistance improving agent of the present invention is not particularly limited, Emulsions, wettable powders, liquids, aqueous solutions, powders, and pastes commonly used in the relevant technical field. It may be in the form of a compound or granules. In each embodiment of the present invention, acetic acid, etc., is included or applied in an agriculturally effective amount. In each embodiment, the amount of agriculturally effective acetic acid, etc., is, for example, 0.0 of the total mass at the time of application. The range is 1 to 0.5% by mass, and typically it is 0.05 to 0.5% by mass relative to the total mass at the time of application. This ranges from 0.075% to 0.25% by mass relative to the total mass at the time of application. This range is more typically 0.09 to 0.2% by mass relative to the total mass at the time of application. This is a range, specifically within the range of 0.1 to 0.2% by mass relative to the total mass at the time of application. If the heat-resistant or salt-resistant agent for plants of the present invention is in liquid form, then acetic acid, etc. The amount that is agriculturally effective is, for example, in the range of 0.01 to 0.5 volume percent of the total volume at the time of application. Yes, and typically it is in the range of 0.05 to 0.5 volume percent of the total volume at the time of application. The amount is in the range of 0.075 to 0.25 volume percent relative to the total volume at the time of application, and furthermore, typical Specifically, the range is 0.09 to 0.2 volume percent of the total mass at the time of application, and in particular at the time of application This is in the range of 0.1 to 0.2 volume percent relative to the total mass.

[0034] In plant cultivation, the heat resistance or salt resistance of the plant according to the present invention is determined based on the state of plant growth. The performance enhancer is applied to plants, materials used for applying to plants, or the soil, growing medium, or By appropriately setting the conditions for application to the culture solution, the heat tolerance or salt tolerance of plants can be improved. It is possible to improve the plant growth while stably managing it. The present invention Obtaining one or more pieces of information related to plant growth (hereinafter also referred to as the "information acquisition process") do), Based on one or more pieces of information obtained, the plant heat resistance or salt resistance improver of the present invention is used in plant Materials for application to objects, plants, or for application to the soil, growing medium, or nutrient solution in which plants grow. Determining the conditions (hereinafter also referred to as the "determination of application conditions process"), This also relates to methods for managing plant growth, including [specific examples of plant growth management].

[0035] In the information acquisition process, one or more pieces of information related to plant growth are obtained, and are not limited to Although not definitively established, for example, under heat stress or salt stress conditions, young stems and leaves Alternatively, root elongation, increase in leaf number, promotion of flowering or fruiting, increase in the number of flowers or fruits, Regarding effects that promote growth, such as increasing plant weight or crop yield, greening, or promoting tillering. Various information, as well as information regarding inability to grow (death), poor growth (for example, the entire plant or its parts Whitening or yellowing of parts (for example, leaves or flowers), reduction in root length or reduction in the number of leaves, (This refers to plant growth problems such as lodging, decreased growth rate, or a decrease in plant weight or crop yield.) Various pieces of information regarding undesirable effects in this regard can be cited. By obtaining one or more pieces of information as exemplified above, the growth status of the plant can be evaluated. It is possible.

[0036] The heat-resistant or salt-resistant plant agent of the present invention, determined in the application condition determination process, The conditions for application are that the heat tolerance or salt tolerance of the plant will be improved by the application. The conditions are set as appropriate so that improvements can be made. The conditions determined in this process are particularly The composition of the heat-resistant or salt-resistant plant enhancer of the present invention is, for example, not limited to the present invention. This consists of pH, application rate and timing, and the content of acetic acid and other active ingredients. One or more conditions can be selected from the group. The specific values ​​for these conditions may be set as appropriate from the ranges exemplified herein.

[0037] In the present invention, in a method for managing plant growth, the process includes an information acquisition step and a determination of application conditions. The number and order of steps are not particularly limited. For example, the information acquisition step and the application condition determination step are... This process may be carried out once in this order: information acquisition process, application condition determination process, and then further The information acquisition process may be carried out in this order, with the first information acquisition process and the first determination of application conditions being performed. The process of determining the fixed process, the second information acquisition process, and the second application condition determination process, etc. The combination of the application condition determination process may be repeated multiple times. [Examples]

[0038] The present invention will be described in more detail below using examples. However, the technical scope of the present invention is These examples are not the only ones.

[0039] <Heat Resistance Test 1> In a simply constructed incubator, using plastic pots, lettuce (sanchi (Lactuca sativa L., Asteraceae family) After germination, at a temperature of 22°C The plants were grown for two weeks under conditions of ℃ and 40-50% humidity. During the growing period, watering was done using a watering tray. Then, I watered the plants from the bottom so that the bottom of the plastic pots was submerged in water. Next, excess water from bottom watering was removed by gravity on a dry watering tray. Water or a 0.1% by volume aqueous acetic acid solution was used for bottom watering via a watering tray for 24 hours. This bottom watering treatment allows water or an acetic acid solution to be supplied to the lettuce plant from the bottom of the vinyl pot. It will be watered. Afterward, the plastic pots were moved to a separate tray and left without water, under conditions of continuous light and a temperature of 35°C. The samples were then left undisturbed in an incubator for four days. After 4 days of standing, remove the plastic pot from the incubator and place it at a temperature of 22°C. The plants were then watered from the bottom, and the condition of the lettuce was observed after 24 hours. The results are shown in Figure 1. In addition, in the following experiments, lettuce and tomatoes were grown under continuous light, Except for the illuminance in the incubator under the condition of a temperature of 42°C, which was set to approximately 3300 lux, the other conditions were approximately 5 The condition was set at 000 lux.

[0040] <Heat Resistance Test 2> For the lettuce plants grown in the same manner as in Heat Resistance Test 1, 50 mL of water was applied per plant. A 0.1% by volume aqueous acetic acid solution was poured around the base of the plant and left to stand for 24 hours. This pouring treatment resulted in the development of a vi Water or an acetic acid solution will be supplied to the lettuce plant from the soil surface inside the Neilpot. Afterward, without supplying water, the plants were kept in an incubator under conditions of continuous light and a temperature of 42°C for 3 days. I placed it down. After 3 days of standing, remove the plastic pot from the incubator and place it at a temperature of 22°C. The plants were watered from the bottom, and the survival rate of the lettuce was measured after two days. The results are shown in Figure 2.

[0041] <Heat Resistance Test 3> In a simple incubator, using plastic pots, tomatoes (variety: Momota) are being grown. After germination, the Solanum lycopersicum (a species of the nightshade family) is grown at a temperature of 22°C. The plants were grown for three weeks under conditions of 40-50% humidity. During the growing period, watering was done using a watering tray. Bottom watering was performed so that the bottom of the plastic pot was submerged in water. Next, excess water from bottom watering was removed by gravity on a dry watering tray. Drench the base of each plant with 50 mL of water or a 0.06% by volume aqueous acetic acid solution and let stand for 24 hours. This irrigation treatment supplied water or an acetic acid solution to the tomatoes from the soil surface inside the plastic pots. It will be watered. Afterward, without supplying water, the plants were kept in an incubator under conditions of continuous light and a temperature of 42°C for 3 days. I placed it down. After 3 days of standing, remove the plastic pot from the incubator and place it at a temperature of 22°C. The tomatoes were watered from the bottom, and the survival rate was measured after two days. Survival rate of tomatoes in the acetic acid solution watering group. The survival rate was 100%, while the survival rate of tomatoes in the water-supplied group was 0%. The results are shown in Figure 3.

[0042] <Salt Tolerance Test> For the lettuce plants grown in the same manner as in Heat Resistance Test 1, 100 mL of water was added per plant. The sample was irrigated with a 0.1 vol% aqueous acetic acid solution or a 0.2 vol% aqueous acetic acid solution and left to stand for 24 hours. During the injection process, water or an acetic acid solution is supplied to the lettuce plant from the soil surface inside the plastic pot. This will happen. Pour 100 mL of 3.5% sodium chloride solution into the base of each plant and leave it to stand for 3 days. Next, 100 mL of a 3.5% sodium chloride solution was poured around the base of the plant. The condition of the lettuce plants was observed 7 days after the initial irrigation with a sodium chloride solution. The results are shown in Figure 4.

Claims

1. A plant containing acetic acid or its salts, or solvates thereof, for heat resistance or salt resistance. An additive.

2. The heat-resistant or It is a salt resistance improver.

3. The heat resistance or salt resistance improving agent according to claim 2, wherein the pH is in the range of 3 to 9.

4. The claim 2 or 3, which contains acetic acid or a salt thereof in an amount ranging from 0.01 to 0.5 volume percent. Heat resistance or salt resistance improver.

5. A plant containing acetic acid or its salts, or solvates thereof, for heat resistance or salt resistance. A composition for improving [something].

6. The composition according to claim 5, further comprising one or more solvents, including at least water.

7. The composition according to claim 6, wherein the pH is in the range of 3 to 9.

8. The claim 6 or 7 contains acetic acid or a salt thereof in an amount ranging from 0.01 to 0.5 volume percent. composition.

9. Acetic acid or its salts, or solvates thereof, plants, materials for application to plants, This includes applying it to the soil, growing medium, or culture solution in which the plant grows, and improving the heat resistance of the plant. This is a method to improve salt resistance.

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