Flow channel unit for heater, heater, and culture apparatus

Abstract

This invention provides a flow channel unit for a heater that can improve the heating performance of the liquid supplied to the culture vessel. [Solution] A flow path unit used in a heater connected to a culture vessel for heating a liquid supplied to the culture vessel, comprising a bag body having an inlet for the liquid to flow in, an outlet for the liquid to flow out, and a flow path for circulating the liquid that has flowed in from the inlet toward the outlet, wherein the bag body comprises a surface sheet made of a laminated film having a first sealant layer on the innermost side, a back sheet made of a laminated film having a second sealant layer on the innermost side and facing the surface sheet, a peripheral seal portion at the periphery where the first sealant layer and the second sealant layer are joined, and an inner seal portion inside the peripheral seal portion where the first sealant layer and the second sealant layer are joined, wherein the flow path comprises the peripheral seal portion, the inner seal portion and the inner surface of the bag body.

Description

Technical Field

[0001] The present invention relates to a flow path unit for a warmer, a warmer, and a culture device.

Background Art

[0002] Conventionally, a culture device for culturing cells, microorganisms, etc. in a culture container is known. Liquids such as liquid media used for culturing often need to be stored refrigerated, and when in use, for example, since it is necessary to warm to a predetermined temperature such as the temperature for culturing, in a culture device, a configuration for warming the liquid before supplying it to the culture container has been proposed.

[0003] For example, in Patent Document 1, a first container containing a medium, a second container for culturing cells, a first liquid feeding pipe and a first liquid feeding mechanism for feeding the medium in the first container to the second container, a first temperature adjusting unit for heating or cooling the liquid in the second container, and between the first container and the second container, a cell culture device having a second temperature adjusting unit for heating or cooling the medium in the first container is disclosed. Further, it is described that the second temperature adjusting unit can be installed in the first liquid feeding pipe and the first liquid feeding pipe can be wound in a spiral shape.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] However, in the cell culture device described in Patent Document 1, improvement in the warming efficiency of the liquid supplied to the culture container has not been considered, and when warming a large amount of liquid and supplying it to the culture container, there is a possibility that the liquid cannot be sufficiently warmed to a predetermined temperature before being supplied to the culture container.

[0006] In view of the above, one aspect of the present invention aims to provide a flow channel unit for a heater that can improve the heating performance of the liquid supplied to the culture vessel. [Means for solving the problem]

[0007] A flow channel unit for a heater according to one aspect of the present invention is a flow channel unit used in a heater connected to a culture vessel for heating a liquid supplied to the culture vessel, and comprises a bag body having an inlet for the liquid to flow in, an outlet for the liquid to flow out, and a flow channel for circulating the liquid that has flowed in from the inlet toward the outlet, wherein the bag body comprises a surface sheet made of a laminated film having a first sealant layer on the innermost side, a back sheet made of a laminated film having a second sealant layer on the innermost side and facing the surface sheet, a peripheral seal portion at the periphery where the first sealant layer and the second sealant layer are joined, and an inner seal portion inside the peripheral seal portion where the first sealant layer and the second sealant layer are joined, and the flow channel comprises the peripheral seal portion, the inner seal portion and the inner surface of the bag body. [Effects of the Invention]

[0008] According to one aspect of the present invention, a flow channel unit for a heater can improve the heating performance of the liquid supplied to the culture vessel. [Brief explanation of the drawing]

[0009] [Figure 1] This is a perspective view of a culture apparatus equipped with a heater according to one embodiment. [Figure 2] This is a perspective view showing a heater equipped with a flow channel unit for a heater according to one embodiment. [Figure 3] This is a cross-sectional view II in Figure 2. [Figure 4] This is a perspective view of a flow path unit for a heater according to one embodiment. [Figure 5] This is a diagram showing the II-II section of Figure 4. [Figure 6]This is a schematic diagram of the cross-section of the surface sheet of the bag body of a flow channel unit for a heater according to one embodiment. [Figure 7] This is a perspective view showing another example of a flow channel unit for a heater according to one embodiment. [Modes for carrying out the invention]

[0010] Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings. In order to facilitate understanding of the description, the same reference numerals are used for the same components in each drawing, and redundant explanations may be omitted. In this specification, the "~" indicating a numerical range means that the values ​​written before and after it are included as the lower and upper limits, respectively, unless otherwise specified. In this specification, a three-dimensional Cartesian coordinate system with three axes (X direction, Y direction, Z direction) may be used, and on a plane parallel to the surface of the heater 3, one of two mutually orthogonal directions may be defined as the Y direction and the other as the Z direction, and the direction perpendicular to the surface of the heater 3 may be defined as the X direction. Note that the orientation of the heater 1 when in use is not limited to the examples shown in Figures 1 and 2.

[0011] Figure 1 is a perspective view of a culture apparatus equipped with a heater according to one embodiment. As shown in Figures 1 to 3, the heater 1 according to one embodiment of the present invention is connected to a culture vessel 11 and heats the liquid L supplied to the culture vessel 11. Examples of the liquid L supplied to the culture vessel 11 include liquid culture medium, pure water, and chemical solutions. The heater 1 is detachable from the culture vessel 11.

[0012] As shown in Figure 1, the culture apparatus 10 comprises a culture vessel 11 and a heater 1 connected to the culture vessel 11.

[0013] The culture vessel 11 is a container for performing culture, containing the target organism to be cultured, such as cells or microorganisms, a liquid culture medium such as a culture solution for culturing the target organism, and gases necessary for culture. The culture vessel 11 may be a single-use container. The shape of the culture vessel 11 may be, for example, a rectangle or a circle in plan view.

[0014] The culture container 11 may be a bag-shaped container made of a flexible packaging material such as film. The warmer 1 is detachable from the culture container 11. Specifically, the culture container 11 is a sealable bag-shaped container and may have a sealing portion provided on its periphery. The culture container 11 may be a gusseted bag, in which case the shape of the culture container 11 may be cylindrical or prismatic.

[0015] From the viewpoint of allowing the contents sealed inside the culture container 11 to be visible from the outside, it is preferable that the culture container 11 be partially or entirely transparent. When the culture container 11 is a bag-shaped container, the base material constituting the flexible packaging material is not particularly limited, but examples include polyolefins such as polyethylene, polypropylene, and polystyrene, thermoplastic resins such as polyamide and polyester, or laminates thereof. The culture container 11 may also be a laminate in which a biaxially oriented nylon film or a biaxially oriented polyethylene terephthalate (PET) film is laminated on the aforementioned base material as a reinforcing layer. This can improve the strength of the culture container 11. The culture container 11 may also be a laminate in which a vapor-deposited layer made of silica or alumina, or a resin layer made of all or part of an ethylene-vinyl alcohol copolymer or polyvinyl acetate is laminated on the aforementioned base material or reinforcing layer as a gas barrier layer. This can improve the gas barrier properties of the culture container 11.

[0016] The culture container 11 may be constructed by layering two or three layers of flexible packaging material to more securely prevent leakage of its contents. In this case, it is preferable that the inner and outer layers of the flexible packaging material contain thermoplastic resin. By layering and sealing two or three sheets of flexible packaging material, the culture container 11, which is a bag-shaped container constructed by layering two or three layers of flexible packaging material, can be formed.

[0017] The thickness of the substrate constituting the culture vessel 11 is not particularly limited and can be appropriately selected according to the material of the substrate and the capacity of the culture vessel 11. For example, the thickness of the substrate may be 50 μm to 1000 μm. The capacity of the culture vessel 11 is not particularly limited, but can be, for example, 0.1 L to 1000 L.

[0018] The culture vessel 11 has a liquid inlet 12 to which the warmer 1 is connected. The liquid inlet 12 is an inlet for allowing a liquid L such as a liquid medium for culturing to flow into the culture vessel 11. It may have a gas outlet 13. When the culture vessel 11 has an upper surface, a lower surface, and a side surface, the liquid inlet 12 may be provided on the upper surface or the lower surface of the culture vessel 11. According to this configuration, in the culture apparatus 10, the liquid L used for culturing can be made to flow from the warmer 1 into the culture vessel 11 through the liquid inlet 12, and the operation can be performed while maintaining a closed system until the culturing is completed.

[0019] The culture vessel 11 may have a gas outlet 13. The gas outlet 13 is an outlet for discharging gases such as the supplied gas and carbon dioxide generated by culturing.

[0020] The liquid inlet 12 and the gas outlet 13 may be ports. The material constituting the port is not particularly limited, and examples include resin, metal, etc. The port can be inserted into an opening formed in the culture vessel 11, and can be attached to the culture vessel 11 by fixing a flange portion in a flange shape to the inner surface of the culture vessel 11 with an adhesive, heat fusion, or the like.

[0021] The culture apparatus 10 may have a pipe 14 having one end connected to the liquid inlet 12 and the other end connected to the warmer 1. The culture apparatus 10 may further have a pipe 15 having one end connected to the gas outlet 13. The material constituting the pipe 14 and the pipe 15 is not particularly limited, and examples include silicone, thermoplastic elastomer, metal, etc. The shapes of the pipe 14 and the pipe 15 are preferably variable.

[0022] The culture apparatus 10 may include on-off valves provided in the middle of the pipe 14 and the pipe 15, respectively. The on-off valves allow the inflow and cutoff of gas into the culture vessel 11, and maintain the culture vessel 11 in a sealed state.

[0023] The culture vessel 11, piping 14, piping 15, and ports are preferably sterilized. Sterilization methods can be selected according to the purpose of culture, etc., and examples include radiation such as gamma rays, gas such as ethylene oxide, heating with water vapor, etc.

[0024] Figure 2 is a perspective view showing a heater equipped with a heating channel unit according to one embodiment, Figure 3 is a cross-sectional view II of Figure 2, Figure 4 is a perspective view of a heating channel unit according to one embodiment, and Figure 5 is a cross-sectional view II-II of Figure 4. As shown in Figures 2 to 4, the heater 1 comprises a heating channel unit 2 and a heater 3.

[0025] The heating channel unit 2 is connected to the culture vessel 11 and is used in the heating unit 1 to heat the liquid L supplied to the culture vessel 11. The heating channel unit 2 comprises a bag body 20 having an inlet 241 into which the liquid L flows, an outlet 251 into which the liquid L flows out, and a channel 23 that causes the liquid L flowing in from the inlet 241 to flow toward the outlet 251. The heater 3 is positioned opposite at least one side of the bag body 20, i.e., at least one of the front and back surfaces. The inlet 241 is connected to, for example, a container containing a liquid culture medium. The liquid L flowing in from the inlet 241 into the channel 23 is heated by the heat from the heater 3 as it flows through the channel 23 and reaches the outlet 251.

[0026] As shown in Figure 3, the bag body 20 comprises a front sheet 21 and a back sheet 22 facing the front sheet 21.

[0027] The bag body 20 may be formed by folding a single sheet, or by laminating multiple sheets together. That is, the surface sheet 21 and the back sheet 22 may be formed from a single sheet, or they may be formed from separate sheets. At least one of the surface sheet 21 and the back sheet 22 located in the flow path 23 may be in contact with the heater 3 in part or in whole. As shown in Figure 3, the surface sheet 21 and the back sheet 22 located in the flow path 23 may each be in contact with the heater 3 in part.

[0028] As shown in Figure 5, the surface sheet 21 is composed of a laminated film having a first sealant layer 211 on the innermost side, and the back sheet 22 is composed of a laminated film having a second sealant layer 221 on the innermost side.

[0029] Specifically, the surface sheet 21 can be composed of a laminated film in which a first sealant layer 211 and a base material layer 212 are laminated. From the viewpoint of thermal fusion properties, it is preferable that the material constituting the first sealant layer 211 is the same type of material as the material constituting the first mouth member 24 and the second mouth member 25, which will be described later.

[0030] Examples of materials constituting the first sealant layer 211 include polyethylene and polypropylene. Preferably, the material constituting the first sealant layer 211 is polypropylene. By using polypropylene as the material for the first sealant layer 211, the sealed portion (heat-sealed portion) of the bag 20 can achieve high adhesive strength in high-temperature environments, and the bag 20 can have appropriate rigidity. Unstretched polypropylene (CPP) can be suitably used as the polypropylene.

[0031] The material constituting the first sealant layer 211 more preferably includes block polypropylene. This suppresses the penetration of liquid L from the first sealant layer 211 to the substrate layer 212.

[0032] The first sealant layer 211 may consist of one layer or multiple layers. When the first sealant layer 211 consists of multiple layers, it is preferable to have a three-layer structure with two layers of random polypropylene sandwiching a layer of block polypropylene. The layer of block polypropylene has the function of suppressing the penetration of liquid L into other adjacent layers, and the layer of random polypropylene has the function of firmly adhering to the other layers. Therefore, by adopting the above three-layer structure, the durability of the bag 20 can be improved.

[0033] The base layer 212 may consist of one layer or multiple layers. Figure 6 is a schematic cross-sectional view of the surface sheet of a bag according to one embodiment. The base layer 212 can consist of multiple layers, for example, as shown in Figure 6, in which a metal layer 212a, resin layers 212b, and 212c are sequentially laminated on a first sealant layer 211.

[0034] The metal layer 212a can be, for example, a metal foil or a metal vapor-deposited film. Examples of materials that make up the metal layer 212a include aluminum, copper, stainless steel, and titanium. Among these, it is preferable that the material making up the metal layer 212a includes aluminum. This allows the heater 1 to further improve its heating performance.

[0035] The thickness of the metal layer 212a is preferably 10 nm or more, and more preferably 30 nm or more. A thickness of 30 nm or more for the metal layer 212a allows the heater 1 to further improve its heating performance and also provides good handling. The thickness of the metal layer 212a is preferably 1 μm or less, and more preferably 80 nm or less. A thickness of 80 nm or less for the metal layer 212a allows the bag 20 to have sufficient flexibility to adhere closely to the heater 3.

[0036] In the example shown in Figure 6, the resin layer consists of two layers, but it may consist of one layer or three or more layers. When the resin layers 212b and 212c consist of two layers, for example, the material constituting the inner resin layer 212b may be nylon, and the material constituting the outer resin layer 212c may be polyethylene terephthalate. By using nylon as the material constituting the resin layer 212b, the bag 20 can improve its pressure resistance when liquid L is circulated through the flow path 23, and the occurrence of pinholes can be suppressed. Furthermore, by using polyethylene terephthalate as the material constituting the resin layer 212c, the bag 20 can improve its scratch resistance. Examples of materials constituting the resin layers 212b and 212c include nylon, polyethylene terephthalate, polyester, and the like.

[0037] Each layer constituting the surface sheet 21 may be joined via an adhesive layer or an anchoring agent layer, or it may be directly joined. The surface sheet 21 may have other layers besides the metal layer 212a and the resin layers 212b and 212c on the outside of the first sealant layer 211.

[0038] The structure of the back sheet 22 is the same as that of the front sheet 21 described above, so its explanation is omitted. Also, the structure of the second sealant layer 221 is the same as that of the first sealant layer 211 described above, so its explanation is omitted.

[0039] The thickness of the surface sheet 21 and the back sheet 22 is preferably 50 μm or more and 350 μm or less, and more preferably 150 μm or more and 300 μm or less.

[0040] As shown in Figure 4, the bag 20 has a peripheral seal portion S1 where the first sealant layer 211 and the second sealant layer 221 are joined at the periphery, and an inner seal portion S2 inside the peripheral seal portion S1 where the first sealant layer 211 and the second sealant layer 221 are joined. The peripheral seal portion S1 and the inner seal portion S2 are, for example, seal portions where the first sealant layer 211 and the second sealant layer 221 are heat-fused. The bag 20 may be a pouch container.

[0041] The channel 23 is composed of a peripheral sealing portion S1, an inner sealing portion S2, and the inner surface of the bag body 20. In the example shown in Figure 4, the channel 23 has a wave shape with four folded portions 23a, 23b, 23c, and 23d in a plan view. The shape of the channel 23 in a plan view may be, for example, a rectangular wave shape, a sinusoidal wave shape, or a triangular wave shape. That is, the shape of the channel 23 in a plan view may be a shape in which U shapes are repeated, or a shape in which V shapes are repeated.

[0042] The bag body 20 may include a first opening member 24 having an inlet 241 and a second opening member 25 having an outlet 251. Each of the first opening member 24 and the second opening member 25 is provided between the surface sheet 21 and the back sheet 22 and is heat-fused to the first sealant layer 211 and the second sealant layer 221. That is, the inlet 241 and the outlet 251 open in a direction (Y direction) along the surface of the heater 3 or the surface of the bag body 20. For example, a pipe 14 can be connected to the second opening member 25.

[0043] The materials constituting the first opening member 24 and the second opening member 25 can be suitably used if they can be joined with the first sealant layer 211 and the second sealant layer 221 to ensure airtightness, and are preferably materials that can be heat-fused to the first sealant layer 211 and the second sealant layer 221. From the viewpoint of heat fusion properties, the materials constituting the first opening member 24 and the second opening member 25 are more preferably the same type of material as the materials constituting the first sealant layer 211 and the second sealant layer 221.

[0044] The first opening member 24 and the second opening member 25 may be cylindrical members and may have a flange portion extending outward from one end. The first opening member 24 and the second opening member 25 may be spouts. By having spouts with flange portions, the contact area between each of the first opening member 24 and the second opening member 25 and the first sealant layer 211 and the second sealant layer 221 is increased, thereby increasing the adhesive strength between them. The shape of the bag body 20 may be, for example, a rectangle in plan view.

[0045] The bag 20 in this embodiment may have a thermometer. The thermometer may be installed inside or outside the bag 20. Installing the thermometer inside the bag 20 allows for more accurate measurement of the liquid L temperature. Alternatively, installing the thermometer outside the bag 20 allows for repeated use of the thermometer by simply replacing the bag 20.

[0046] The thermometer is preferably installed at or near the outlet 251 of the bag 20. This allows the temperature of the liquid L heated by the heater 1 to be measured. Furthermore, if the temperature of the liquid L measured by the thermometer is lower than the target temperature, the pump discharge rate can be reduced to increase the time the liquid L stays in the heater 1, thereby improving the temperature of the liquid L flowing out of the outlet 251 of the bag 20. The pump discharge rate may be controlled by computer-based PID control or the like. By using PID control, a liquid L at a constant temperature can be discharged from the outlet 251 of the bag 20.

[0047] It is preferable that the heater 3, in a plan view (when viewed from the X direction), overlaps with at least the flow path 23 of the bag 20. The heater 3 may be arranged in pairs facing each other on the front and back surfaces of the bag 20. The heater 1 may have a support member between the pair of heaters 3 that supports the pair of heaters 3 at a distance from each other. This makes it possible to maintain the gap between the pair of heaters 3 in which the bag 20 is placed.

[0048] The heater 3 is not particularly limited as long as it can heat the liquid in the flow path, and can be a hot air heater, infrared heater, electromagnetic wave heater, electric heater, hot water heater, etc. Electric heaters and hot water heaters can be used in contact with the surface sheet 21 or back sheet 22 that form the flow path.

[0049] If the culture apparatus is equipped with a hot water heater or the like, it is possible to share the hot water heater between the heater 1 and the culture vessel 11 in this embodiment.

[0050] The shape of the heater 3 may be plate-like. The surface of the heater 3 that contacts the bag 20 may be flat or have a corrugated shape that conforms to the flow path of the bag 20.

[0051] Figure 7 is a perspective view showing another example of a heating fluid channel unit according to one embodiment. The heating fluid channel unit 2 may have a sterile connection connector 26 connected to at least one of the inlet 241 and the outlet 251, as shown in Figure 7. Here, "connection" means direct or indirect connection. Furthermore, the heating fluid channel unit 2 may have a tube 27 provided between at least one of the inlet 241 and the outlet 251 and the sterile connection connector 26. The heating fluid channel unit 2 may have a tube 27 provided at the end of the sterile connection connector 26.

[0052] Another embodiment involves a heating device 1 using a bag 20 having an inlet 241 and an outlet 251, and a heater 3 having a flow channel shape on the surface in contact with the bag 20. In this case, the bag 20 only needs to have an inlet 241 and an outlet 251, and it is not necessary for the bag itself to have a complex flow channel. The load of the heater 3, which has a flow channel shape on the surface in contact with the bag 20, and the pressure applied to the bag 20 cause the bag 20 to expand due to the pressure of the liquid L entering from the inlet 241, resulting in the formation of a complex flow channel.

[0053] Even if the heater 3 does not have a flow channel shape on the surface in contact with the bag 20, the internal space of the bag 20 will still form a single flow channel.

[0054] The heater 1 of this embodiment is not limited to the culture apparatus 10 with the configuration shown in Figure 1, but can be applied to any culture apparatus.

[0055] As described above, the heating channel unit 2 of this embodiment comprises a bag body 20, the bag body 20 having a surface sheet 21, a back sheet 22, a peripheral seal portion S1, and an inner seal portion S2, and the channel 23 is composed of the peripheral seal portion S1, the inner seal portion S2, and the inner surface of the bag body 20. With this configuration, the heating channel unit 2 can heat the channel 23 through which the liquid L flows from the side. In addition, since the width of the inner seal portion S2 can be made relatively small, the ratio of the area of ​​the channel 23 to the bag body 20 can be increased. Furthermore, since the bag body 20 comprises the channel 23, the channel 23 and the heater of the heating device come into contact more easily compared to when the channel is composed of pipes or the like. Therefore, the heating channel unit 2 can improve heating performance.

[0056] Furthermore, in this embodiment, compared to forming a flexible tube by bending it, it is possible to make the flow path cross-sectional area of ​​the bent portion and the flow path cross-sectional area of ​​the other portions more uniform. As a result, pressure loss is reduced, and the discharge capacity of the pump can be utilized more effectively.

[0057] Furthermore, the heating element flow path unit 2 offers a high degree of design flexibility because its size and shape can be changed to match the size and shape of the heater.

[0058] Furthermore, the heating element flow channel unit 2 allows for the replacement of the bag 20 with a new bag 20, and contamination can be suppressed even when changing the liquid supplied to the culture vessel.

[0059] In this embodiment, the bag body 20 comprises a first opening member 24 and a second opening member 25, each of which is provided between the surface sheet 21 and the back sheet 22 and is heat-fused to the first sealant layer and the second sealant layer 221. With this configuration, each of the first opening member 24 and the second opening member 25 is heat-fused to the first sealant layer 211 and the second sealant layer 221, and is joined to the first sealant layer 211 and the second sealant layer 221 with sufficient adhesive strength. Therefore, the heating flow channel unit 2 can suppress leakage of liquid L from the bag body 20 and maintain heating performance.

[0060] In this embodiment, each of the surface sheet 21 and the back sheet 22 includes a metal layer. This allows the heating channel unit 2 to improve heat exchange efficiency, thereby further improving heating performance.

[0061] The heating channel unit 2 of this embodiment has a sterile connection connector 26 connected to at least one of the inlet 241 and the outlet 251. With this configuration, the heating channel unit 2 can further suppress contamination even when replacing the bag 20 with a new bag 20 and changing the liquid supplied to the culture vessel.

[0062] The heating channel unit 2 of this embodiment has a tube 27 provided between at least one of the inlet 241 and outlet 251 and the sterile connection connector 26. With this configuration, the heating channel unit 2 can further suppress contamination even when replacing the bag 20 with a new bag 20 and changing the liquid supplied to the culture vessel.

[0063] The heater 1 of this embodiment comprises a heater flow channel unit 2 and a heater 3. With this configuration, the heater 1 can heat the flow channel 23 through which the liquid L flows from the side. In addition, since the width of the inner seal portion S2 can be made relatively small, the ratio of the area of ​​the flow channel 23 to the bag body 20 can be increased. Furthermore, since the bag body 20 is equipped with a flow channel 23, the flow channel 23 and the heater 3 are more likely to come into contact compared to when the flow channel is composed of pipes or the like. Therefore, the heating performance can be improved with the culture device 10.

[0064] In this embodiment, it is preferable that at least one of the surface sheet 21 and the back sheet 22 located in the flow path 23 is in contact with the heater 3, either partially or entirely. This configuration makes it easier for heat from the heater 3 to be transferred into the flow path 23, thereby improving the heating performance of the heater 1.

[0065] The culture apparatus 10 in this embodiment comprises a culture container 11 and a heater 1 connected to the culture container 11. The heater 1 can heat from the side of the flow path 23 through which the liquid L flows. Furthermore, since the width of the inner seal portion S2 can be made relatively small, the ratio of the area of ​​the flow path 23 to the bag body 20 can be increased. Moreover, since the bag body 20 is equipped with a flow path 23, the flow path 23 and the heater 3 are more likely to come into contact compared to when the flow path is composed of pipes or the like. Therefore, the culture apparatus 10 can improve heating performance.

[0066] (Aspects of the present invention) The present invention includes the following embodiments. <Aspect 1> A flow path unit used in a heater connected to a culture vessel for heating the liquid supplied to the culture vessel, The bag comprises an inlet into which the liquid flows, an outlet from which the liquid flows out, and a flow path that causes the liquid flowing in from the inlet toward the outlet. The bag body comprises a surface sheet made of a laminated film having a first sealant layer on the innermost side, It is composed of a laminated film having a second sealant layer on the innermost side, and a back sheet facing the surface sheet, At the periphery, the first sealant layer and the second sealant layer are joined together to form a periphery seal portion, Inward from the peripheral seal portion, there is an inner seal portion where the first sealant layer and the second sealant layer are joined together. The aforementioned flow path is a flow path unit for a heater, comprising the peripheral seal portion, the inner seal portion, and the inner surface of the bag body. <Aspect 2> The bag comprises a first opening member having the inlet and a second opening member having the outlet. The heating channel unit according to Embodiment 1 is provided between the surface sheet and the back sheet, and heat-fused to the first sealant layer and the second sealant layer, respectively. <Aspect 3> Each of the aforementioned surface sheet and the aforementioned back sheet is a heating channel unit according to embodiment 1 or 2, which includes a metal layer. <Aspect 4> A flow path unit for a heater according to any one of embodiments 1 to 3, having a sterile connection connector connected to at least one of the inlet and outlet. <Aspect 5> This is a heating fluid channel unit according to embodiment 4, having a tube provided between at least one of the inlet and outlet and the sterile connection connector. <Aspect 6> A heater connected to a culture vessel, which heats the liquid supplied to the culture vessel, A heating channel unit according to any one of embodiments 1 to 5, The heating device comprises a heater positioned opposite at least one side of the bag body. <Aspect 7> The heater according to embodiment 6, wherein at least one of the surface sheet and the back sheet located in the flow path is in contact with the heater in part or in whole. <Aspect 8> Culture vessel and The culture apparatus comprises a heater according to embodiment 6 or 7 connected to the culture vessel.

[0067] As described above, embodiments have been explained, but these embodiments are presented as examples only, and the present invention is not limited by these embodiments. The above embodiments can be implemented in various other forms, and various combinations, omissions, substitutions, and modifications are possible without departing from the spirit of the invention. These embodiments and their variations are included in the scope and spirit of the invention, as well as in the claims of the invention and its equivalents. [Explanation of Symbols]

[0068] 1 Warmer 2. Flow channel unit for heater 20 Bag body 21 Surface sheet 211 First sealant layer 213 Through hole 22 Back sheet 221 Second sealant layer 23 Flow channels 241 Inlet 251 Outlet 26. Sterile connection connectors 27 tubes 3 Heaters 10 Culture device 11 Culture vessel S1 Peripheral seal portion S2 Inner seal section

Claims

1. A flow path unit used in a heater connected to a culture vessel for heating the liquid supplied to the culture vessel, The bag comprises an inlet into which the liquid flows, an outlet from which the liquid flows out, and a flow path that causes the liquid flowing in from the inlet toward the outlet. The bag body comprises a surface sheet made of a laminated film having a first sealant layer on the innermost side, It is composed of a laminated film having a second sealant layer on the innermost side, and a back sheet facing the surface sheet, At the periphery, the first sealant layer and the second sealant layer are joined together to form a periphery seal portion, Inward from the peripheral seal portion, there is an inner seal portion where the first sealant layer and the second sealant layer are joined together. The flow path is a heating flow path unit comprising the peripheral sealing portion, the inner sealing portion, and the inner surface of the bag body.

2. The bag comprises a first opening member having the inlet and a second opening member having the outlet. The heating channel unit according to claim 1, wherein each of the first port member and the second port member is provided between the surface sheet and the back sheet and is heat-fused to the first sealant layer and the second sealant layer.

3. The heating channel unit according to claim 1, wherein each of the surface sheet and the back sheet includes a metal layer.

4. A flow path unit for a heater according to claim 1, having a sterile connection connector connected to at least one of the inlet and outlet.

5. A flow path unit for a heater according to claim 4, comprising a tube provided between at least one of the inlet and the outlet and the sterile connection connector.

6. A heater connected to a culture vessel, which heats the liquid supplied to the culture vessel, A flow path unit for a heater according to any one of claims 1 to 5, A heater comprising a heater positioned opposite at least one side of the bag body.

7. The heater according to claim 6, wherein at least one of the surface sheet and the back sheet located in the flow path is in contact with the heater in part or in whole.

8. Culture vessel and A culture apparatus comprising a heater according to claim 6, connected to the culture vessel.

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