Method for promoting hair growth

Abstract

To provide a new method for promoting hair growth or hair restoration.SOLUTION: A method for promoting hair growth or hair restoration in the skin 10 comprises to irradiate the skin with at least one of first light and second light unlike the first light. In the method, the first light applied from a first light source 2 is the light where a peak wavelength region is 550 nm or more and 600 nm or less, and a half value width is 50 nm or less. The second light applied from a second light source 3 is the light where the peak wavelength region is 1100 nm or more and 1400nm or less, and the half value width is 200 nm or less.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] The present invention relates to a method for promoting hair growth or development on the skin. [Background technology]

[0002] 2. Description of the Related Art In order to promote hair growth or hair development on the scalp, devices using light-emitting diodes (LEDs) that emit light having a specific wavelength range have been proposed (for example, Patent Documents 1 and 2). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent No. 5540324 [Patent Document 2] JP 2018-68482 A Summary of the Invention [Problem to be solved by the invention]

[0004] The hair growth promoting devices described in Patent Documents 1 and 2 both utilize light with a peak wavelength range of 630 nm or more and 660 nm or less.

[0005] An object of the present invention is to provide a new method for promoting hair growth or hair development, which utilizes light having a wavelength range other than the above-mentioned wavelength ranges. [Means for solving the problem]

[0006] As a result of intensive research to solve the above problems, the present inventors have unexpectedly found that irradiating hair follicle keratinocytes, which are epithelial cells that create hair follicles, with light having a peak wavelength range of 550 nm to 600 nm, or light having a peak wavelength range of 1100 nm to 1400 nm, promotes the proliferation of hair follicle keratinocytes. The present invention is based on this finding and provides the following inventions.

[0007] [1] A method for promoting hair growth or hair development on skin, comprising irradiating the skin with at least one of a first light and a second light different from the first light, the first light has a peak wavelength range of 550 nm or more and 600 nm or less, and a half width of 50 nm or less; The method according to claim 1, wherein the second light has a peak wavelength range of 1100 nm or more and 1400 nm or less, and a half width of 200 nm or less. [2] further comprising irradiating the skin with a third light; The method according to [1], wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half-value width of 50 nm or less. [3] 1. A method for promoting proliferation of hair follicular keratinocytes in skin, comprising irradiating skin with at least one of a first light and a second light different from the first light, the first light has a peak wavelength range of 550 nm or more and 600 nm or less, and a half width of 50 nm or less; The method according to claim 1, wherein the second light has a peak wavelength range of 1100 nm or more and 1400 nm or less, and a half width of 200 nm or less. [4] A method for promoting production of at least one factor selected from the group consisting of platelet-derived growth factor (PDGF) A, WNT5A, and placental growth factor (PGF) in skin, comprising irradiating the skin with a third light, The method, wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half-width of 50 nm or less. [5] A method for promoting gene expression in skin of at least one factor selected from the group consisting of platelet-derived growth factor (PDGF) A, WNT5A, and placental growth factor (PGF), comprising irradiating the skin with a third light, The method, wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half-width of 50 nm or less. [6] A light irradiation device for irradiating light to skin, The present invention provides a lighting device that includes at least one of a first light source that emits a first light and a second light source that emits a second light different from the first light, the first light has a peak wavelength range of 550 nm or more and 600 nm or less, and a half width of 50 nm or less; The second light has a peak wavelength range of 1100 nm or more and 1400 nm or less, and a half width of 200 nm or less. [7] a first light source and a second light source; The light irradiation device according to [6], further comprising a drive unit that irradiates the first light and the second light simultaneously or alternately. [8] Further comprising a third light source that emits a third light, The light irradiation device according to [6] or [7], wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half-value width of 50 nm or less. [9] Further comprising a third light source that emits a third light, The light irradiation device according to [8], further comprising a drive unit that simultaneously or alternately irradiates at least one of the first light and the second light and the third light. Effect of the Invention

[0008] According to the present invention, it is possible to provide a new method for promoting hair growth or hair development using light having a specific peak wavelength range. Also, according to the present invention, it is possible to provide a light irradiation device that irradiates light having the specific peak wavelength range. [Brief description of the drawings]

[0009] [Figure 1] 1 is a graph showing the proliferation effect of hair follicle keratinocytes when irradiated with light of each peak wavelength in Test Example 1. [Diagram 2]1 is a graph showing the effect of promoting the production of hair-related factors when irradiated with light of each peak wavelength in Test Example 3. [Diagram 3] 1A and 1B are diagrams illustrating light irradiation to the skin by a light irradiation device according to an embodiment of the present invention. [Figure 4] 1A and 1B are diagrams illustrating light irradiation to the skin by a light irradiation device according to an embodiment of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] Hereinafter, an embodiment of the present invention will be described in detail. However, the present invention is not limited to the following embodiment.

[0011] 1. Method for promoting hair growth or development on the skin A first embodiment of the present invention is a method for promoting hair growth or hair development on skin, comprising irradiating the skin with at least one of a first light and a second light different from the first light. In the first embodiment, the first light is light having a peak wavelength range of 550 nm or more and 600 nm or less, and a half width of 50 nm or less, and the second light is light having a peak wavelength range of 1100 nm or more and 1400 nm or less, and a half width of 200 nm or less.

[0012] Hereinafter, "skin" in this specification means human or animal skin. "Skin" is preferably human skin, more preferably human scalp.

[0013] The peak wavelength range of the first light may be 550 nm or more and 600 nm or less, and the half width of the first light may be 50 nm or less. By irradiating the skin with the first light having this peak wavelength range and half width, the proliferation of hair follicle keratinocytes present in the skin is promoted, and hair growth or hair development in the skin is promoted. The peak wavelength range of the first light is preferably 560 nm or more and 590 nm or less, more preferably 565 nm or more and 585 nm or less. In addition, the half width of the first light is preferably 40 nm or less, more preferably 30 nm or less. The first light can be irradiated by using a light irradiation device described later that is equipped with a light source such as an LED capable of irradiating a predetermined light that satisfies the above conditions.

[0014] The peak wavelength range of the second light may be 1100 nm or more and 1400 nm or less, and the half width of the second light may be 200 nm or less. By irradiating the skin with the second light having this peak wavelength range and half width, the proliferation of hair follicle keratinocytes present in the skin is promoted, and hair growth or hair development in the skin is promoted. The peak wavelength range of the second light is preferably 1150 nm or more and 1350 nm or less, more preferably 1200 nm or more and 1300 nm or less. The half width of the second light is preferably 150 nm or less, more preferably 100 nm or less. The second light can be irradiated by using a light irradiation device described later that is equipped with a light source such as an LED capable of irradiating a predetermined light that satisfies the above conditions.

[0015] In the first embodiment, the light irradiated to the skin may be only the first light, may be only the second light, or may be the first light and the second light. In addition, in the first embodiment, when the first light and the second light are irradiated to the skin, these two kinds of light may be irradiated simultaneously or alternately.

[0016] The irradiation energy of the first light and / or the second light is not particularly limited as long as it is necessary for promoting hair growth or hair development on the skin and has an intensity that does not cause damage to the skin such as roughness, inflammation, etc. The irradiation energy of the first light and / or the second light can be adjusted by, for example, adjusting the intensity of the light emitted from the light source in the light irradiation device, the distance between the light source in the light irradiation device and the skin, etc.

[0017] The time for irradiating the skin with the first light and / or the second light is not particularly limited as long as it is necessary for promoting hair growth or hair development on the skin and does not cause damage to the skin such as rough skin or inflammation. The irradiation time of the first light is preferably 5 minutes or more and 60 minutes or less, more preferably 10 minutes or more and 30 minutes or less. The irradiation time of the second light is preferably 5 minutes or more and 60 minutes or less, more preferably 10 minutes or more and 30 minutes or less. The irradiation of the first light and / or the second light can be performed continuously, or irradiation for a certain period of time and non-irradiation for a certain period of time can be repeated.

[0018] As described above, the proliferation of hair follicle keratinocytes present in the skin is promoted by irradiating the skin with at least one of a first light and a second light different from the first light.Therefore, as one embodiment of the present invention, there is provided a method for promoting the proliferation of hair follicle keratinocytes in the skin, comprising irradiating the skin with at least one of a first light and a second light different from the first light, wherein the first light is light having a peak wavelength range of 550 nm or more and 600 nm or less and a half width of 50 nm or less, and the second light is light having a peak wavelength range of 1100 nm or more and 1400 nm or less and a half width of 200 nm or less.

[0019] A second embodiment of the present invention is a method for promoting hair growth or hair development in the skin, which further comprises irradiating the skin with a third light in addition to irradiating the skin with at least one of a first light and a second light different from the first light. In the second embodiment, the first light and the second light are the same as the first light and the second light in the first embodiment, and the third light is light having a peak wavelength range of 610 nm or more and 660 nm or less and a half width of 50 nm or less.

[0020] The peak wavelength range of the third light may be 610 nm or more and 660 nm or less, and the half width of the third light may be 50 nm or less. By further irradiating the skin with the third light having this peak wavelength range and half width, gene expression of hair-related factors (specifically, for example, platelet-derived growth factor A (PDGFA), WNT5A, placenta growth factor (PGF)) present in the skin is promoted, and the production of hair-related factors is promoted, and hair growth or hair development in the skin is further promoted in combination with the promotion of proliferation of hair follicle keratinocytes by the irradiation of the first light and / or the second light. The peak wavelength range of the third light is preferably 615 nm or more and 655 nm or less, more preferably 620 nm or more and 650 nm or less. The half width of the third light is preferably 40 nm or less, more preferably 30 nm or less. The third light can be irradiated by using a light irradiation device described later that is equipped with a light source such as an LED capable of irradiating a predetermined light that satisfies the above conditions.

[0021] In the second embodiment, the light irradiated to the skin may be the first light and the third light, the second light and the third light, or the first light, the second light and the third light. In the second embodiment, these two or three types of light may be irradiated simultaneously or alternately.

[0022] The irradiation energy of the third light is not particularly limited as long as it is necessary for promoting hair growth or hair development on the skin and has an intensity that does not cause damage to the skin such as roughness, inflammation, etc. The irradiation energy of the third light can be adjusted, for example, by adjusting the intensity of light emitted from the light source in the light irradiation device, the distance between the light source in the light irradiation device and the skin, etc.

[0023] The time for irradiating the skin with the third light is not particularly limited as long as it is necessary for promoting hair growth or hair development on the skin and does not cause damage to the skin such as rough skin or inflammation. The irradiation time of the third light is preferably 5 minutes or more and 60 minutes or less, more preferably 10 minutes or more and 30 minutes or less. The irradiation of the third light can be performed continuously, or can be performed by repeating irradiation for a certain period of time and non-irradiation for a certain period of time.

[0024] As described above, by irradiating the skin with the third light, gene expression of hair-related factors present in the skin is promoted. Therefore, as one embodiment of the present invention, there is provided a method for promoting gene expression of at least one factor selected from the group consisting of platelet-derived growth factor A (PDGFA), WNT5A, and placental growth factor (PGF) in the skin, comprising irradiating the skin with the third light, wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half-width of 50 nm or less. Furthermore, by irradiating the skin with the third light, production of hair-related factors present in the skin is promoted. Therefore, as one embodiment of the present invention, there is provided a method for promoting production of at least one factor selected from the group consisting of platelet-derived growth factor A (PDGFA), WNT5A, and placental growth factor (PGF) in the skin, comprising irradiating the skin with the third light, wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half-width of 50 nm or less.

[0025] [2. Light irradiation device that irradiates light onto the skin] A third embodiment of the present invention is a light irradiation device that irradiates light to skin, comprising at least one of a first light source that irradiates a first light and a second light source that irradiates a second light different from the first light, wherein the first light has a peak wavelength range of 550 nm or more and 600 nm or less and a half width of 50 nm or less, and the second light has a peak wavelength range of 1100 nm or more and 1400 nm or less and a half width of 200 nm or less.

[0026] For example, as shown in FIG. 3, the light irradiation device according to the third embodiment includes a first light source 2, a second light source 3, and a driving unit 1 that drives the first light source 2 and the second light source 3. The first light source 2 is an LED or the like that can irradiate the skin 10 with a predetermined light that satisfies the above conditions as the first light, and may be adjusted by arranging grading or the like as necessary. The second light source 3 is an LED or the like that can irradiate the skin 10 with a predetermined light that satisfies the above conditions as the second light, and may be adjusted by arranging grading or the like as necessary. The driving unit 1 includes a driving circuit or the like that can apply a predetermined voltage to the first light source 2 and the second light source 3.

[0027] Furthermore, the light irradiation device according to the third embodiment may include at least one of a first light source 2 and a second light source 3, and the driving unit 1 may drive at least one of the first light source 2 and the second light source 3.

[0028] The fourth embodiment of the present invention is a light irradiation device for irradiating light to skin, which further includes a third light source for irradiating a third light in addition to at least one of a first light source for irradiating a first light and a second light source for irradiating a second light different from the first light. In this embodiment, the first light and the second light are synonymous with the first light and the second light in the third embodiment, and the third light is light having a peak wavelength range of 610 nm or more and 660 nm or less and a half width of 50 nm or less.

[0029] For example, as shown in FIG. 4, the light irradiation device according to the fourth embodiment may include a third light source 4 in addition to the first light source 2 and the second light source 3, and the driving unit 1 may drive the first light source 2, the second light source 3, and the third light source 4. The first light source 2 is an LED or the like capable of irradiating the skin 10 with a predetermined light that satisfies the above conditions as the first light, and may be adjusted by arranging grading or the like as necessary. The second light source 3 is an LED or the like capable of irradiating the skin 10 with a predetermined light that satisfies the above conditions as the second light, and may be adjusted by arranging grading or the like as necessary. The third light source 4 is an LED or the like capable of irradiating the skin 10 with a predetermined light that satisfies the above conditions as the third light, and may be adjusted by arranging grading or the like as necessary. The driving unit 1 includes a driving circuit or the like capable of applying a predetermined voltage to the first light source 2, the second light source 3, and the third light source 4.

[0030] In addition, the light irradiation device according to the fourth embodiment may include at least one of the first light source 2 and the second light source 3, and a third light source 4, and the driving unit may include at least one of the first light source 2 and the second light source 3, and the third light source 4.

[0031] The light irradiation device according to this embodiment can be suitably used to promote hair growth or hair development on the skin (preferably the human scalp). EXAMPLES

[0032] The present invention will be specifically described below based on test examples, but the present invention is not limited to these.

[0033] [Test Example 1: Evaluation of hair follicle keratinocyte proliferation] Human hair follicle keratinocytes (HHFK) (ScienCell Research Laboratories, Inc., product number 2440) were added at 1 × 10 4The cells were seeded in a 24-well plate at 1000 cells / well and cultured for 24 hours. The medium was replaced with HBSS, and the cells were irradiated with LEDs emitting light with each peak wavelength and half-width for 2 hours (peak wavelength (half-width): 572 nm (20 nm), 1200 nm (71 nm), and 1300 nm (71 nm)). After irradiation, the medium was replaced with HBSS and the cells were cultured for another 24 hours. The medium was replaced with HBSS again, and the cells were irradiated with LEDs emitting light with each of the above peak wavelengths for 2 hours. After irradiation, the medium was replaced with HBSS, and the cells were cultured for another 48 hours, after which the cell proliferation effect of HHFK was evaluated using the WST-8 method. As a control, HHFK obtained by the same method as above except that no LED irradiation was used. The results are shown in Figure 1.

[0034] As shown in FIG. 1, cell proliferation was confirmed when the cells were irradiated with light having a peak wavelength of 572 nm, 1200 nm, or 1300 nm.

[0035] [Test Example 2: Evaluation of gene expression of hair-related factors using dermal papilla cells] Human hair follicle papilla cells (HFDPC) (Cell Applications, Inc., product number CA60205a) were added at 5 × 10 4 The cells were seeded in a 24-well plate at 100 cells / well and cultured for 48 hours. The medium was replaced with HBSS, and the cells were irradiated with LEDs emitting light with each peak wavelength and half-width for 3 hours (peak wavelength (half-width): 623 nm (20 nm) and 636 nm (20 nm)). After irradiation, the medium was replaced with HBSS, and the cells were cultured for another 24 hours. After the culture, the cells were collected, and the gene expression of hair growth or hair growth related factors (PDGFA, WNT5A, and PGF) was measured by real-time PCR. As a control, cells obtained in the same manner as above except that they were not irradiated with LED were used. The results, expressed as a percentage of the expression level in the cells irradiated with light having each peak wavelength relative to the expression level in the control, are shown in Table 1.

[0036] [Table 1]

[0037] As shown in Table 1, it was confirmed that irradiation with light having peak wavelengths of 623 nm and 636 nm promoted gene expression for all of the factors PDGFA, WNT5A, and PGF.

[0038] [Test Example 3: Evaluation of the production of hair-related factors using dermal papilla cells] Human hair follicle papilla cells (HFDPC) (Cell Applications, Inc., product number CA60205a) were added at 5 × 10 4 The cells were seeded in a 24-well plate at 1000 cells / well and cultured for 48 hours. The medium was replaced with HBSS, and the cells were irradiated with an LED emitting light having each peak wavelength for 2 hours (peak wavelength (half width): 623 nm (20 nm) and 636 nm (20 nm)). After irradiation, the medium was replaced with HBSS and the cells were cultured for another 24 hours. The medium was replaced with HBSS again, and the cells were irradiated with an LED emitting light having each of the above peak wavelengths for 2 hours. After irradiation, the medium was replaced with HBSS and the cells were cultured for another 24 hours. The culture supernatant was collected, and the amount of PGF produced in the culture supernatant was measured by ELISA using a Human PLGF ELISA Kit (manufactured by Abcam; product number ab100629). In addition, after collecting the culture supernatant, a cell nucleus stain (Hoechst33342 (DOJINDO; product number H342)) diluted 1000-fold with medium was added to the 24-well plate, and 30 minutes later, the nuclei were counted using an imaging device to obtain the cell number. The amount of PGF produced per cell count was calculated, and this was taken as the productivity. As a control, cells obtained in the same manner as above, except that they were not irradiated with LED, were used. The results are shown in Figure 2.

[0039] As shown in FIG. 2, it was confirmed that irradiation with light having a peak wavelength of 623 nm or 636 nm promoted the production of PGF. [Explanation of symbols]

[0040] 1...drive unit, 2...first light source, 3...second light source, 4...third light source, 10...skin.

Claims

1. A light irradiation device for irradiating light to skin, The present invention provides a lighting device that includes a first light source that emits a first light and a second light source that emits a second light different from the first light, the first light has a peak wavelength range of 550 nm or more and 600 nm or less and a half width of 50 nm or less; The second light has a peak wavelength range of 1200 nm or more and 1300 nm or less, and a half width of 100 nm or less.

2. The light irradiation device according to claim 1 , further comprising a drive unit that irradiates the first light and the second light simultaneously or alternately.

3. The light irradiation device described in Claim 2, wherein the driving unit alternately irradiates the first light and the second light.

4. Further comprising a third light source that emits a third light, 4. The light irradiation device according to claim 1, wherein the third light has a peak wavelength range of 610 nm or more and 660 nm or less, and a half width of 50 nm or less.

5. Further comprising a third light source that emits a third light, The light irradiation device according to claim 4 , further comprising a drive unit that simultaneously or alternately irradiates at least one of the first light and the second light and the third light.

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