Fat absorption inhibitor, food, defatted sesame, and method for inhibiting obesity

Abstract

To provide a novel fat absorption inhibitor.SOLUTION: The present invention is a fat absorption inhibitor comprising finely pulverized product of defatted sesame or a processed product thereof. Further, the present invention is a fat absorption inhibitor comprising defatted sesame having a 50% particle diameter of 1 to 200 μm as measured by a laser diffraction light scattering method and a compression degree of 30 to 80%. The fat absorption preferably has a lipase inhibitory ability. The present invention is a food product comprising the fat absorption inhibitor. The present invention is defatted sesame having a 50% particle diameter of 1 to 200 μm as measured by a laser diffraction light scattering method and a compression degree of 30 to 80%. The present invention is a method for inhibiting obesity of a human being or an animal comprising feeding the fat absorption inhibitor, the food product, or the defatted sesame to a human being or an animal.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a fat absorption inhibitor, a food product, defatted sesame, and a method for inhibiting obesity. [Background technology]

[0002] Due to changes in diet and lifestyle, the number of obese people is increasing worldwide. Obesity is a risk factor that increases various health risks, such as diabetes, high blood pressure, and cancer, and it is said that four million people die annually from diseases caused by obesity worldwide.

[0003] Anti-obesity drugs currently in use include (1) those that act on the appetite center to suppress appetite (mazindol, etc.), and (2) those that suppress fat absorption by inhibiting intestinal lipase (orlistat, etc.). Many of these anti-obesity drugs have some kind of side effects, and their safety has not yet been established, so few can be used long-term.

[0004] Under these circumstances, it has been studied to obtain safer fat absorption inhibitors by inhibiting lipase as described in (2) above by using food materials. Food materials known to have lipase inhibitory activity include Eucommia leaves (Patent Document 1), Balsam, Mitsumouka, Sea Spruce, Chinese Cabbage, Capsicum anguicum (Patent Document 2), black ginger, red ginger, Cinnamon, young barley leaves, black garlic, Asarum oleifera, hydrangea, linseed, pomegranate, linseed, osmanthus flower, nine-section tea, snowdrop, jasmine tea, oregano, olive leaves, seaweed, curry leaves (Patent Document 3), and white vine vine (Patent Document 4). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] JP 2005-289951 A [Patent Document 2] JP 2006-169181 A [Patent Document 3] JP 2010-209051 A [Patent Document 4] JP 2014-172901 A Summary of the Invention [Problem to be solved by the invention]

[0006] However, many of the above-mentioned food materials are not widely distributed on the market, which leads to the inconvenience of high costs and reduced supply. Therefore, from the viewpoints of availability and cost reduction, there is a demand for fat absorption inhibitors using more familiar food materials.

[0007] The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a novel fat absorption inhibitor. [Means for solving the problem]

[0008] As a result of intensive research into solving the above problems, the inventors discovered that finely ground defatted sesame seeds exhibited a fat absorption inhibitory effect, and continued their research based on this finding, which led to the completion of the present invention.

[0009] The invention made to solve the above problems is a fat absorption inhibitor containing finely ground defatted sesame seeds or a processed product thereof.

[0010] Another invention made to solve the above problems is a fat absorption inhibitor containing defatted sesame having a 50% particle size of 1 to 200 μm as measured by a laser diffraction light scattering method and a compression degree of 30 to 80%.

[0011] Still another invention made to solve the above problems is defatted sesame having a 50% particle size of 1 to 200 μm as measured by a laser diffraction light scattering method and a compression degree of 30 to 80%.

[0012] Yet another invention made to solve the above problems is a method for suppressing obesity in humans or animals, which comprises administering the agent, the food, or the defatted sesame to humans or animals.

[0013] More specifically, the present invention relates to the following inventions: [1] A fat absorption inhibitor containing finely ground defatted sesame seeds or processed products thereof. [2] A fat absorption inhibitor containing defatted sesame seeds having a 50% particle size of 1 to 200 μm and a compression degree of 30 to 80% as measured by a laser diffraction light scattering method. [3] The agent according to [1] or [2], which has lipase inhibitory activity. [4] A food product containing the agent according to any one of [1] to [3]. [5] Defatted sesame having a 50% particle size of 1 to 200 μm as measured by a laser diffraction light scattering method and a compression degree of 30 to 80%. [6] A method for suppressing obesity in a human or animal, comprising administering to a human or animal the agent described in any one of [1] to [3], the food described in [4], or the defatted sesame described in [5]. Effect of the Invention

[0014] According to the present invention, a novel fat absorption inhibitor can be provided. The fat absorption inhibitor of the present invention is obtained from a food material, and therefore is highly safe. The fat absorption inhibitor of the present invention has excellent fat absorption inhibitory ability and lipase inhibitory ability. The fat absorption inhibitor of the present invention can be easily produced without requiring a complicated production process. The food of the present invention contains the fat absorption inhibitor of the present invention, and therefore has fat absorption inhibitory ability and lipase inhibitory ability. The defatted sesame of the present invention can be suitably used as a raw material for the fat absorption inhibitor of the present invention. Therefore, by administering the agent, food and defatted sesame of the present invention to humans or animals, obesity of humans or animals can be suppressed. In addition, the present invention can be suitably used for the treatment or prevention of diseases caused by fat accumulation such as obesity and serum hypertriglyceridemia, and diseases such as diabetes, hyperlipidemia, hypertension, and arteriosclerosis caused by obesity, as well as for pharmaceuticals, foods, food additives, functional foods, etc. for dieting. [Brief description of the drawings]

[0015] [Figure 1] 1 is a graph showing the results of a fat absorption inhibition test (in humans). [Diagram 2] 1 is a graph showing the results of a fat absorption inhibition test (in vivo) of unground defatted sesame seeds. [Diagram 3] 1 is a graph showing the results of a fat absorption inhibition test (in vivo) of finely ground defatted sesame seeds. [Figure 4] 1 is a graph showing the results of a lipase inhibition test (in vitro). [Diagram 5] 1 is a graph showing the results of a long-term intake test (in vivo). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0016] <Fat absorption inhibitor> The fat absorption inhibitor includes those having the effect of inhibiting the transfer of lipids such as neutral fats (triglycerides) into the blood (fat absorption inhibitory ability), and the fat absorption inhibitor usually has lipase inhibitory ability.

[0017] The fat absorption inhibitor of the present invention may be embodied in the following forms (1) to (4). (1) A fat absorption inhibitor containing finely ground defatted sesame seeds or processed products thereof. (2) A fat absorption inhibitor containing defatted sesame seeds having a 50% particle size of 1 to 200 μm as measured by a laser diffraction light scattering method. (3) A fat absorption inhibitor containing defatted sesame seeds having a compression rate of 30 to 80%. (4) A fat absorption inhibitor containing defatted sesame having a 50% particle size of 1 to 200 μm and a compression degree of 30 to 80% as measured by a laser diffraction light scattering method.

[0018] The fat absorption inhibitor contains, as an essential component, "finely ground defatted sesame or a processed product thereof," and preferably contains "defatted sesame having a 50% particle size of 1 to 200 μm and a compression degree of 30 to 80% as measured by a laser diffraction light scattering method." The fat absorption inhibitor may contain other optional components within the range that does not impair the effects of the present invention. Each component will be described below.

[0019] [Finely ground defatted sesame seeds or processed products thereof] A fat absorption inhibitor according to one embodiment of the present invention contains finely ground defatted sesame seeds or a processed product thereof.

[0020] The finely ground defatted sesame is preferably obtained by grinding defatted sesame (unground) to even smaller particles than the "ground" which is usually obtained by grinding defatted sesame (unground) to a smaller average particle size. The average particle size is preferably 1 / 2 or less, more preferably 1 / 4 or less, of that of the unground sesame, and it is particularly preferable that the average particle size is, for example, 200 μm or less as the 50% particle size.

[0021] The term "processed product" in the context of finely ground defatted sesame means a product that has been physically and / or chemically processed. Examples of processed products of finely ground defatted sesame include extracts obtained by subjecting finely ground defatted sesame to extraction processing, concentrates of the extracts, residual finely ground products, dried products, frozen products, and the like.

[0022] Examples of the extraction solvent used in the extraction process include alcohols such as ethanol and ethanol aqueous solution, ketones such as acetone, ethers such as diethyl ether, esters such as ethyl acetate, and hydrocarbons such as toluene. The lower limit of the temperature of the extraction process is preferably 0°C, more preferably 20°C. The upper limit of the temperature of the extraction process is preferably 100°C, more preferably 70°C. The lower limit of the time of the extraction process is preferably 10 seconds, more preferably 1 minute. The upper limit of the time of the extraction process is preferably 5 hours, more preferably 1 hour. The lower limit of the mass ratio of the extraction solvent to the defatted sesame finely ground product in the extraction process (extraction solvent / defatted sesame finely ground product) is preferably 0.1, more preferably 1. The upper limit of the mass ratio (extraction solvent / defatted sesame finely ground product) is preferably 100, more preferably 10. The present disclosure includes any combination of these lower and upper limits.

[0023] Methods for obtaining a concentrate from the extract obtained by the extraction treatment include, for example, atmospheric concentration and reduced pressure concentration.

[0024] (Degreased sesame) It was found that defatted sesame does not have the ability to inhibit fat absorption when left unground, but when finely ground, it exhibits the ability to inhibit fat absorption.

[0025] "Defatted sesame" refers to sesame raw material that has been defatted. Examples of sesame raw materials include black sesame, white sesame, golden sesame, etc. Roasted sesame may be used as the sesame raw material. The method for roasting the sesame raw material may be performed according to a known method.

[0026] The method for degreasing the sesame raw material includes, for example, a pressing method, a solvent extraction method such as a hexane extraction method, etc. The method for degreasing the sesame raw material may be carried out according to a known method.

[0027] As the defatted sesame (unground), for example, those having an oil content of 35% by mass or less that are generally available on the market can be used. As a commercially available product of the defatted sesame (unground), for example, "Sesame Oil Cake" by Polars Laboratories can be mentioned.

[0028] The upper limit of the fat content of the defatted sesame (unground) is preferably 35% by mass, more preferably 30% by mass, even more preferably 25% by mass, and particularly preferably 15% by mass. The lower limit of the fat content of the defatted sesame is preferably 1% by mass, more preferably 2% by mass, even more preferably 5% by mass, and particularly preferably 8% by mass.

[0029] The average particle size of defatted sesame (unground) is not particularly limited as a 50% particle size, but is usually 250 μm to 3 mm, preferably 300 μm to 1 mm, and more preferably 400 to 800 μm.

[0030] Methods for obtaining finely ground defatted sesame include grinding defatted sesame (unground) using a grinding machine such as a ball mill, tube mill, jet mill, hammer mill, grinding mill, pin mill, air sweep mill, disk mill, vibration mill, stone mill, or planetary motion mill.

[0031] The lower limit of the temperature when defatted sesame seeds are finely ground is preferably -30°C, more preferably 0°C. The upper limit of the temperature when defatted sesame seeds are finely ground is preferably 100°C, more preferably 50°C. The lower limit of the time for finely grinding defatted sesame seeds is preferably 10 seconds, more preferably 1 minute. The upper limit of the time for finely grinding defatted sesame seeds is preferably 5 hours, more preferably 1 hour.

[0032] The fat absorption inhibitor of another embodiment of the present invention contains defatted sesame seeds having a 50% particle size of 1 to 200 μm as measured by a laser diffraction light scattering method. If the 50% particle size of the defatted sesame seeds exceeds the upper limit, the fat absorption inhibitory ability decreases and sufficient fat absorption inhibitory ability is not exhibited. If the 50% particle size of the defatted sesame seeds is less than the lower limit, the flowability decreases significantly and good operability cannot be maintained.

[0033] The lower limit of the 50% particle size of the defatted sesame contained in the fat absorption inhibitor is preferably 1 μm, more preferably 10 μm, even more preferably 20 μm, and particularly preferably 40 μm. The upper limit of the 50% particle size of the defatted sesame is preferably 200 μm, more preferably 180 μm, even more preferably 160 μm, and particularly preferably 140 μm. By setting the 50% particle size of the defatted sesame within the above range, the fat absorption inhibitory ability can be further improved.

[0034] In this specification, the "50% particle size measured by the laser diffraction light scattering method" is measured, for example, using a laser diffraction type particle size distribution measuring device (Shimadzu Corporation's "SALD-2200", Malvern Instruments' "Mastersizer 3000", etc.) faithfully following the measurement procedure described in the instruction manual of the measuring device. "50% particle size" means "median size (D50)".

[0035] The fat absorption inhibitor of another embodiment of the present invention contains defatted sesame seeds having a degree of compression of 30 to 80%. If the degree of compression of the defatted sesame seeds exceeds the upper limit, the fluidity is significantly reduced and good operability is not maintained. If the degree of compression of the defatted sesame seeds is less than the lower limit, the fat absorption inhibitory ability is reduced and sufficient fat absorption inhibitory ability is not exhibited.

[0036] The lower limit of the compression degree of the defatted sesame contained in the fat absorption inhibitor is preferably 30%, more preferably 35%, even more preferably 40%, and particularly preferably 45%. The upper limit of the compression degree of the defatted sesame is preferably 80%, more preferably 75%, even more preferably 70%, and particularly preferably 65%. By setting the compression degree of the defatted sesame in the above range, the fat absorption inhibitory ability can be further improved.

[0037] In this specification, the "compressibility" is calculated from the measured values ​​of the "hardened bulk density" and the "loose bulk density" using, for example, a multi-function powder property measuring device (such as "Multitester MT-02" manufactured by Seishin Enterprise Co., Ltd.) in accordance with the measurement procedures described in the instruction manual of the measuring device, using the following formula: Compressibility (%) = (hard bulk density (g / mL) - loose bulk density (g / mL)) ÷ hard bulk density (g / mL) × 100

[0038] The lower limit of the bulk density of the defatted sesame is preferably 0.50 g / mL, more preferably 0.55 g / mL.The upper limit of the bulk density of the defatted sesame is preferably 0.62 g / mL, more preferably 0.60 g / mL.

[0039] The lower limit of the loose bulk density of the defatted sesame is preferably 0.20 g / mL, more preferably 0.25 g / mL.The upper limit of the loose bulk density of the defatted sesame is preferably 0.40 g / mL, more preferably 0.35 g / mL.

[0040] The lower limit of the dynamic bulk density of the defatted sesame is preferably 0.35 g / mL, more preferably 0.40 g / mL.The upper limit of the dynamic bulk density of the defatted sesame is preferably 0.60 g / mL, more preferably 0.55 g / mL.

[0041] The lower limit of the angle of repose of the defatted sesame is preferably 35°, more preferably 40°, and the upper limit of the angle of repose of the defatted sesame is preferably 60°, more preferably 55°.

[0042] The lower limit of the crumbling angle of the defatted sesame is preferably 15°, more preferably 20°, and the upper limit of the crumbling angle of the defatted sesame is preferably 30°, more preferably 27°.

[0043] The lower limit of the difference angle (angle of repose - angle of collapse) of the defatted sesame is preferably 15°, more preferably 20°, and the upper limit of the difference angle of the defatted sesame is preferably 30°, more preferably 25°.

[0044] The lower limit of the spatula angle of the defatted sesame is preferably 55°, more preferably 60°, and the upper limit of the spatula angle of the defatted sesame is preferably 75°, more preferably 70°.

[0045] A fat absorption inhibitor in another embodiment of the present invention contains defatted sesame having a 50% particle size of 1 to 200 μm and a compression degree of 30 to 80% as measured by a laser diffraction light scattering method.

[0046] By setting the 50% particle size and compression degree of the defatted sesame contained in the fat absorption inhibitor within the above ranges, the fat absorption inhibitory ability can be made even higher.

[0047] (Other optional ingredients) Other optional components that may be contained in the fat absorption inhibitor include, for example, carbohydrate sweeteners (monosaccharides such as fructose, glucose, tagatose, arabinose, etc., oligosaccharides such as lactose, oligosaccharides, trehalose, maltose, etc., powdered starch syrup, dextrin, sugar alcohols, etc.), high-intensity sweeteners (sucralose, acesulfame K, stevia, etc.), polysaccharides such as starch, flavorings such as vanillin, flavoring materials (eggs, coffee, tea, cocoa, fruit juice and pulp, yogurt, alcoholic beverages, etc.), amino acids, proteins, dietary fiber, vitamins, minerals, acidulants, flavorings, oils and fats, dairy products, excipients, preservatives, colorants, acidulants, emulsifiers, agar, etc. These components can be used alone or in combination of two or more.

[0048] The fat absorption inhibitor may be in various forms, such as powder, granules, paste, suspension, etc. The preparation itself of the fat absorption inhibitor and the method for producing the same have been well established, and therefore the present invention may also follow these methods.

[0049] The timing of intake of the fat absorption inhibitor is not particularly limited, but examples include taking it before or after a meal, or simultaneously with a meal, etc. In particular, taking it simultaneously with a meal is preferable in that the fat absorption inhibitory effect can be efficiently obtained.

[0050] When the fat absorption inhibitor is taken before, after or at the same time as a meal, the lower limit of the amount of the fat absorption inhibitor taken per meal is preferably 50 mg, more preferably 100 mg, calculated as defatted sesame finely ground material.The upper limit of the amount of the fat absorption inhibitor taken per meal is preferably 10 g, more preferably 1 g.

[0051] The fat absorption inhibitor can be applied to various foods, as well as to medicines and general industrial products. Among these, application to foods is preferable because the fat absorption inhibitor can be easily and deliciously consumed and can efficiently and simply exert its fat absorption inhibitory effect.

[0052] <Food> The food of the present invention contains the fat absorption inhibitor described above. The method for containing the fat absorption inhibitor may be carried out according to known techniques.

[0053] Examples of such foods include confectionery (chewing gum, candy, tablets, chocolate, jelly, etc.), frozen desserts, starch-based foods such as noodles, bakery foods (bread, biscuits, etc.), oily foods (margarine, shortening, fat spreads, etc.), dairy products (butter, cream, cheese, etc.), drinks (juice, tea, energy drinks), seasonings (soy sauce, sugar, miso, mirin, dressings, sauces, etc.), supplements (protein, tablets, grains, capsules, tablets, powders, granules, etc.), etc. Among these, it is a preferred embodiment to incorporate the fat absorption inhibitor into supplements, noodles, bread, batter for fried foods, etc.

[0054] The lower limit of the content of the fat absorption inhibitor in the food varies depending on the type and purpose of the food, but is preferably 0.0001% by mass, more preferably 0.01% by mass, even more preferably 1% by mass, and particularly preferably 10% by mass, calculated as defatted sesame finely ground material. The upper limit of the content of the fat absorption inhibitor in the food may be 100% by mass. EXAMPLES

[0055] The present invention will be described in detail below with reference to examples, but the present invention is not limited to these.

[0056] [Production Example 1] (Production of Fat Absorption Inhibitor (A)) Defatted sesame (Mitsui & Co., Ltd.'s "Defatted sesame powder") was pulverized using a hammer mill (Hosokawa Micron Corporation's "ACM30") at a rotation speed of 3000 rpm to obtain a finely pulverized defatted sesame. The obtained finely pulverized defatted sesame was used as fat absorption inhibitor (A).

[0057] [Test Example 1: Measurement of 50% particle size and compression degree] (50% particle size measurement) The particle size distribution of defatted sesame (unground) was measured using a laser diffraction particle size distribution analyzer (Shimadzu Corporation's "SALD-2200"). For defatted sesame (unground), the particle size distribution was measured at three points, and the 50% particle size (D50, median size) was 278 μm, 457 μm, and 567 μm. The particle size distribution of the fat absorption inhibitor (A) was measured using a laser diffraction particle size distribution measuring device (Malvern Instruments' "Mastersizer 3000"). For the fat absorption inhibitor (A), the particle size distribution was measured three times, and the 50% particle size (D50, median size) was 32.7 μm, 30.7 μm, and 32.0 μm.

[0058] (Measurement of compression degree) The compressibility of the defatted sesame (unground) and fat absorption inhibitor (A) was measured using a multi-function powder property measuring device (Seishin Enterprise Co., Ltd.'s "Multitester MT-02"). The compressibility was calculated from the measured values ​​of the hard bulk density and loose bulk density using the following compressibility calculation formula.

[0059] <Compression calculation formula> Compressibility (%) = (hard bulk density (g / mL) - loose bulk density (g / mL)) ÷ hard bulk density (g / mL) × 100

[0060] The measured values ​​(loose bulk density, hard bulk density, dynamic bulk density, angle of repose, angle of collapse and spatula angle) for the defatted sesame (unground) and fat absorption inhibitor (A) as well as the calculated values ​​of the compressibility and difference angle are shown in Table 1 below.

[0061] [Table 1]

[0062] The compression degree of defatted sesame (unground) was measured at three points and was 19.0%, 20.7%, and 23.4%. The compressibility of the fat absorption inhibitor (A) was measured at three points and was 52.6%, 53.8%, and 54.2%.

[0063] [Production Example 2] (Production of Fat Absorption Inhibitor (B)) 50 g of the fat absorption inhibitor (A) obtained in the above-mentioned Production Example 1 was subjected to an extraction treatment using 500 mL of a 50% by volume aqueous ethanol solution at 30°C for 180 minutes, and the obtained extract was concentrated under reduced pressure to prepare 7.7 g of fat absorption inhibitor (B).

[0064] [Test Example 2: Fat absorption inhibition test (humans)] For the fat absorption inhibitor (B), a fat absorption inhibition test on humans was carried out by the following method.

[0065] Twenty-six healthy male and female subjects aged 22 years or older and younger than 56 years were divided into two groups: an Active group (13 participants each) and a Placebo group. The active group ingested test food 1 shown in Table 2 below as the test food, and immediately thereafter ingested the high-fat diet (total lipid content: 41.2 g) shown in Table 3 below. The placebo group was administered the same procedure as the active group, except that the test food was test food 2 shown in Table 2 below. Blood was collected 0, 2, 3, 4, and 6 hours after ingestion of the high-fat meal, and blood triglyceride levels were measured. The change in blood triglyceride levels [mg / dL] was calculated from the blood triglyceride levels measured at each time point using the formula below. <Calculation formula for blood triglyceride change> Change in blood triglyceride level = blood triglyceride level after high-fat diet intake - blood triglyceride level before high-fat diet intake

[0066] The composition of each test food used in the fat absorption inhibition test (in humans) is shown in Table 2 below.

[0067] [Table 2]

[0068] Details of the high-fat diet used in the fat absorption inhibition test (humans) are shown in Table 3 below.

[0069] [Table 3]

[0070] The results of Test Example 2 are shown in Figure 1. The change in blood triglyceride levels 3 and 6 hours after ingestion of a high-fat meal was significantly lower in the Active group than in the Placebo group (3 hours: P (significance probability) = 0.04, 6 hours: P = 0.01). Thus, the postprandial fat absorption inhibitory effect of the fat absorption inhibitor (B) in humans was confirmed.

[0071] [Test Example 3: Fat absorption inhibition test for unground defatted sesame seeds (in vivo)] A fat absorption inhibition test for defatted sesame (unground) was conducted on rats using the following method.

[0072] 30 mL of corn oil was mixed with 400 mg of cholic acid, 10 g of cholesterol oleate, and 30 mL of pure water, and the mixture was sonicated for 10 minutes to form an emulsion, which was used to prepare emulsified corn oil for lipid-loaded foods. The rats were weighed, and the dosage was calculated so that 125 mg / kg (weight of defatted sesame (unground) / weight of rat) was administered. The unground defatted sesame used in Production Example 1 was stirred in a 20% (v / v) ethanol solution (50.9 mg / mL) to prepare test solution A. Male Wistar rats (6 weeks old) were divided into two groups, a group that received uncrushed defatted sesame seeds and a control group (7 rats per group). The uncrushed defatted sesame group was orally administered 400 μL of test solution A (dosage of defatted sesame seeds (uncrushed): 20.4 mg (approximately 1 g in human terms (based on a weight of 60 kg))). The control group was orally administered 400 μL of 20% (v / v) ethanol solution. Ten minutes after oral administration, both groups were orally administered 1 mL of emulsified corn oil. Blood was collected every 1.5 hours until 7.5 hours after administration of the lipid-loading diet. The blood was left to stand for 30 minutes and then centrifuged (3000 g x 15 minutes). Serum was removed and blood triglyceride concentration [mg / mL] was measured. Blood triglyceride concentration was measured by laboratory assay. TM The measurement was performed according to the method for measuring triglycerides (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.).

[0073] [Test Example 4: Fat absorption inhibition test for defatted sesame finely ground material (in vivo)] For the fat absorption inhibitor (A), a fat absorption inhibition test was carried out on rats by the following method.

[0074] The body weight of the rats was measured, and the administration dose was calculated so that 125 mg / kg was administered. The fat absorption inhibitor (A) obtained in Production Example 1 was stirred in a 20% (v / v) ethanol solution (45.0 mg / mL) to prepare a test solution B. Male Wistar rats (6 weeks old) were divided into two groups, a defatted sesame finely ground group and a control group (7 rats per group). The defatted sesame finely ground group, which was administered the fat absorption inhibitor (A), was orally administered 400 μL of test solution B (administration dose: 18.8 mg (approximately 1 g / day in human terms)). The control group was orally administered 400 μL of 20% (v / v) ethanol solution. μL of serum was orally administered to each group. Ten minutes after the oral administration, 1 mL of emulsified corn oil was orally administered to each group. Blood samples were taken every 1.5 hours until 7.5 hours after the administration of the lipid-loaded diet. The blood was left to stand for 30 minutes and then centrifuged (3000g x 15 minutes). Serum was extracted and blood triglyceride concentrations [mg / mL] were measured. Blood triglyceride concentrations were measured by laboratory assay. TM The measurement was performed according to the method for measuring triglycerides (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.).

[0075] The results of Test Example 3 are shown in Figure 2. In Test Example 3, one of the subjects in the control group was unable to continue the test and was therefore removed from the control group. No inhibition of fat absorption was observed in the unground defatted sesame. The results of Test Example 4 are shown in Figure 3. The blood triglyceride levels of the defatted sesame finely ground group administered with the fat absorption inhibitor (A) were significantly lower than those of the control group 1.5 hours and 7.5 hours after administration of a lipid-loaded diet (1.5 hours: P=0.03, 7.5 hours: P=0.01), confirming that fat absorption was inhibited. From the results of Test Examples 3 and 4, it was confirmed that the lipid absorption inhibitory effect was exerted by finely grinding defatted sesame (unground).

[0076] [Production Example 3] (Production of Fat Absorption Inhibitor (C)) 50 g of the fat absorption inhibitor (A) obtained in the above-mentioned Manufacturing Example 1 was subjected to an extraction treatment using 500 mL of a 50% by volume aqueous ethanol solution at room temperature for 180 minutes, and the obtained extract was concentrated under reduced pressure to prepare 9 g of fat absorption inhibitor (C).

[0077] [Test Example 5: Lipase inhibition test (in vitro)] The lipase inhibitory activity of the fat absorption inhibitor (C) was evaluated by the following method.

[0078] (Preparation of enzyme solution) Rat intestinal acetone powder 0.5g (Sigma Company A5 mL of 0.1 M citrate buffer (pH 6.0) was added to the lysate (manufactured by Sigma-Aldrich Co., Ltd.), and the mixture was shaken on ice for 1 hour, and then centrifuged at 10,000 rpm for 45 minutes at 4° C. The supernatant was then diluted 100-fold with the citrate buffer to obtain an enzyme solution.

[0079] (Activity evaluation) Lipase Kit S (Pharma Biomedical Co., Ltd.) was used for activity evaluation. 100 μL of aqueous solution (sample) of fat absorption inhibitor (C) of each predetermined concentration, 237 μL of color development solution, 9 μL of enzyme solution, and 4 μL of esterase inhibitor were added to a 24-well plate, and incubated at 30 ° C. for 5 minutes. Then, 25 μL of substrate solution was added, and the reaction was carried out at 30 ° C. for 30 minutes in the dark. Then, 700 μL of reaction stop solution was added, and the absorbance at 412 nm was measured.

[0080] (Control Evaluation) A control was prepared in a 24-well plate with the same sample arrangement. 100 μL (sample) of a fat absorption inhibitor (C) solution of each predetermined concentration, 237 μL of color development solution, 9 μL of enzyme solution, and 4 μL of esterase inhibitor were added, and incubated at 30°C for 35 minutes in the dark. Then, 700 μL of reaction stop solution and 25 μL of substrate solution were added, and the absorbance at 412 nm was measured.

[0081] (calculation) A blank sample was prepared by adding only the solvent instead of the aqueous solution of the fat absorption inhibitor (C). Activity evaluation and control evaluation were also performed on the blank sample. The lipase activity of the sample was calculated according to the following formula. Lipase activity (%)=100×(absorbance of activity evaluation well−absorbance of activity evaluation well (control)) / (absorbance of blank−absorbance of blank (control))

[0082] The results of Test Example 5 are shown in Figure 4. The amount of sample added indicates the final concentration of fat absorption inhibitor (C) in each well. Fat absorption inhibitor (C) has a concentration-dependent and strong lipase inhibitory activity (50% lipase activity inhibitory concentration: IC 50 = 140 μg / mL) was observed.

[0083] [Test Example 6: Long-term ingestion test (in vivo)] A long-term ingestion test (in vivo) on the fat absorption inhibitor was carried out by the following method.

[0084] C57BL / 6 mice (male, 5 weeks old) were divided into two groups, a control group and a pulverized defatted sesame group (10 mice per group). The control group was fed a high-fat diet, and the pulverized defatted sesame group was fed a high-fat diet mixed with 0.1% by mass (approximately 0.5 g / day in human terms) of the pulverized defatted sesame obtained in Production Example 1 (fat absorption inhibitor (A)). The mice were given this diet ad libitum, and their body weights were measured every week for up to 4 weeks.

[0085] The results of Test Example 6 are shown in Figure 5. It was confirmed that long-term intake of the fat absorption inhibitor (A) significantly suppressed weight gain after 21 days (P=0.02) and 28 days (P=0.02) compared to the control group. [Industrial Applicability]

[0086] According to the present invention, a novel fat absorption inhibitor can be provided. The fat absorption inhibitor of the present invention is obtained from a food material, and therefore is highly safe. The fat absorption inhibitor of the present invention has excellent fat absorption inhibitory ability and lipase inhibitory ability. The fat absorption inhibitor of the present invention can be easily produced without requiring a complicated production process. The food of the present invention contains the fat absorption inhibitor of the present invention, and therefore has fat absorption inhibitory ability and lipase inhibitory ability. The defatted sesame of the present invention can be suitably used as a raw material for the fat absorption inhibitor of the present invention. Therefore, by administering the agent, food and defatted sesame of the present invention to humans or animals, obesity of humans or animals can be suppressed. In addition, the present invention can be suitably used for the treatment or prevention of diseases caused by fat accumulation such as obesity and serum hypertriglyceridemia, and diseases such as diabetes, hyperlipidemia, hypertension, and arteriosclerosis caused by obesity, as well as for pharmaceuticals, foods, food additives, functional foods, etc. for dieting.

Claims

1. A fat absorption inhibitor containing finely ground defatted sesame seeds.

2. A fat absorption inhibitor comprising defatted sesame seeds, the defatted sesame seeds having a 50% particle size of 1 to 200 μm as measured by a laser diffraction light scattering method and a compression degree of 30 to 80%.

3. The agent according to claim 1 or 2, which has lipase inhibitory activity.

4. An agent described in any one of claims 1 to 3, which is for food use.

5. A method for suppressing obesity in an animal, which comprises administering the agent according to any one of claims 1 to 4 to the animal.

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