Compositions and methods for thymus regeneration and T cell reconstitution

Abstract

The present invention provides non-thymic endothelial cells (ntECs) genetically engineered to express adenovirus E4ORF1 and / or BMP4, as well as compositions comprising such engineered ntECs. The present invention also provides methods of using the ntECs in therapy, for example, to enhance thymic regeneration (e.g., T cell reconstitution) in subjects in need thereof. Such subjects include patients with damaged thymus, defective thymic function, insufficient T cell production, and / or immunodeficiency, including, for example, patients with aging, infection (e.g., HIV infection), radiotherapy treatment, chemotherapy treatment, or myeloablative conditioning in preparation for organ / tissue transplantation.

Description

[Technical field]

[0001] (CROSS REFERENCE TO RELATED APPLICATIONS) This application claims the benefit of U.S. Provisional Patent Application No. 62 / 853,452, filed May 28, 2019.

[0002] (Sequence Listing) This application contains a Sequence Listing that has been submitted in computer readable form in ASCII format, and is incorporated herein by reference in its entirety. The ASCII copy of the Sequence Listing was created on May 28, 2020 and is approximately 5,546 bytes in size.

[0003] (Incorporated by reference) All documents cited herein are incorporated by reference in their entirety, subject only to jurisdictions that permit incorporation by reference. Additionally, manufacturer's instructions or catalogs for any products cited or referred to herein are also incorporated by reference. Any document incorporated by reference herein, or any teaching therein, may be used in the practice of the present invention. Many of the general teachings provided in U.S. Pat. No. 8,465,732 may be used in conjunction with, or modified for use with, the present invention. Accordingly, the entire contents of U.S. Pat. No. 8,465,732 are incorporated herein by reference. [Background technology]

[0004] The thymus supports the development of T cells derived from hematopoietic progenitor cells mobilized from the bone marrow. Legrand et al. (2007); "Human thymus regeneration and T cell reconstitution;" Seminars in Immunology; Vol. 19; No. 5; pp 280-288. Thymus activity gradually declines with age, resulting in reduced T cell production and impaired immune function. The thymus is also highly sensitive to injury - in various situations, for example, chemotherapy, radiation, pre-transplant treatments prior to organ transplantation (e.g. bone marrow transplantation), and infections (e.g. HIV infection), it is susceptible to damage leading to reduced T cell production and immune deficiency. Regeneration of thymic tissue can replenish the T cell compartment and restore immune function. Although the thymus has some regenerative capacity, the extent and speed of this thymus regeneration is often insufficient, leading to severe immunodeficiency and exposing patients to potentially life-threatening infections. Thus, there is a need in the art for compositions and methods that can enhance both thymus regeneration and T cell reconstitution. The present invention addresses these needs.

[0005] SUMMARY OF THEINVENTION The present invention is based in part on the surprising discovery that thymic regeneration can be induced in vivo by administering to a living subject non-thymic endothelial cells ("ntECs") genetically engineered to express either an adenoviral E4ORF1 polypeptide or both an adenoviral E4ORF1 polypeptide and BMP4. As shown in the Examples herein, administration of engineered ntECs expressing E4ORF1 alone or both E4ORF1 and BMP4 to a living subject promotes thymic regeneration and T cell reconstitution. These effects are particularly surprising given that previously published studies demonstrated that administration of endothelial cells promotes thymic regeneration, but this effect was only achieved using thymic endothelial cells. See Wertheimer et al. (2018); "Production of BMP4 by endothelial cells is crucial for endogenous thymic regeneration;" Sci. Immunol. 3, 2736. In these previous studies, thymic regeneration was not observed when non-thymic endothelial cells were used (ibid.). The discovery that engineered ntECs expressing E4ORF1 alone or both BMP4 and E4ORF1 can be used to induce thymic regeneration and T cell reconstitution in vivo has several important practical implications. One of these is that it obviates the need for complex and invasive procedures to obtain and culture endothelial cells from the patient's thymus. Instead, endothelial cells for thymic regeneration and T cell reconstitution protocols can be obtained from more readily available sources such as adipose tissue, skin tissue or umbilical cord tissue, greatly simplifying the applicability of endothelial cell therapy for thymic regeneration.

[0006] Thus, the present invention provides a variety of novel compositions and methods.

[0007] In one embodiment, the present invention provides a population of genetically engineered ntECs that express BMP4 (i.e., BMP4 +In another embodiment, the present invention provides a population of genetically engineered ntECs that express adenovirus E4ORF1 polypeptides (i.e., E4ORF1 + In another embodiment, the present invention provides a population of genetically engineered ntECs that express BMP4 and adenoviral E4ORF1 polypeptides (i.e., BMP4 + E4ORF1 + In some embodiments, the population of engineered ntECs is an isolated cell population. In some embodiments, the population of engineered ntECs is a substantially pure cell population. In some embodiments, the population of engineered ntECs is present in vitro, e.g., in cell culture. In some embodiments, the population of engineered ntECs is present ex vivo. In some embodiments, the population of engineered ntECs is present in vivo. In some embodiments, the population of engineered ntECs is present in a composition, such as a therapeutic composition, suitable for administration to a living subject. For example, in some embodiments, the invention provides a therapeutic composition comprising a population of engineered ntECs and a saline solution suitable for administration to a living subject. Similarly, in some embodiments, the invention provides a therapeutic composition comprising a population of engineered ntECs and a biocompatible matrix material, such as a liquid biocompatible matrix material (e.g., Matrigel) or a solid biocompatible matrix material.

[0008] In some embodiments, the engineered ntECs provided herein, and / or compositions comprising these engineered ntECs, can be used for a variety of therapeutic applications, such as methods of enhancing thymic regeneration and / or T cell reconstitution in living subjects, such as subjects with deficiencies in thymic tissue mass, thymic function, or T cell production, or subjects with other immunocompromised conditions. In some embodiments, such subjects are elderly, have a viral infection (e.g., HIV infection), have been exposed to radiation (e.g., radiation therapy), have undergone chemotherapy, or have been treated, for example, with myeloablative conditioning agents in preparation for organ transplantation, such as bone marrow or hematopoietic stem cell transplantation (HSCT).

[0009] For example, in some embodiments, the present invention provides a method for enhancing thymus regeneration and / or T cell reconstitution in a subject, the method comprising administering to a subject in need thereof an effective amount of ntECs genetically engineered to express E4ORF1 or both E4ORF1 and BMP4, or a therapeutic composition comprising such ntECs, thereby stimulating thymus regeneration and / or T cell reconstitution in the subject.

[0010] Endothelial cells (ECs) can be obtained from any non-thymic source. Examples of suitable sources of ECs (ntECs) include adipose tissue (i.e., adipose ECs), skin tissue (i.e., skin ECs), heart tissue (i.e., heart ECs), kidney tissue (i.e., kidney ECs), lung tissue (i.e., lung ECs), liver tissue (i.e., liver ECs), bone marrow tissue (i.e., bone marrow ECs), umbilical vein (i.e., umbilical vein ECs--or "UVECs"), and the like.

[0011] In some embodiments, the ntECs are adult ECs. In some embodiments, the ntECs are pediatric ECs. In some embodiments, the ntECs are fetal ECs. In some embodiments, the ntECs are embryonic ECs. In some embodiments, the ntECs are differentiated ECs. In some embodiments, the ntECs are derived from endothelial progenitor cells. In some embodiments, the ntECs are derived from stem cells. In some embodiments, the ntECs are derived from primary tissue cultures. In some embodiments, the ntECs are ECs of an endothelial cell line. When ntECs are to be used in the therapeutic methods described herein, in some embodiments, the ntECs are autologous to the subject to which the cells are to be administered, and in other embodiments, the ntECs are allogeneic to the subject to which the cells are to be administered.

[0012] In some embodiments, the ntECs contain a recombinant nucleic acid molecule that includes a molecule or molecules, such as a nucleotide sequence encoding BMP4 and / or a nucleotide sequence encoding an adenoviral E4ORF1 polypeptide. In embodiments using both BMP4 and E4ORF1, the nucleotide sequence encoding BMP4 and the nucleotide sequence encoding the E4ORF1 polypeptide may be provided in the same recombinant nucleic acid molecule or in separate recombinant nucleic acid molecules. In some embodiments, the nucleotide sequence encoding BMP4 and / or the nucleotide sequence encoding the E4ORF1 polypeptide are operably linked to a heterologous promoter. In some embodiments, the recombinant nucleic acid molecule is a plasmid vector. In some embodiments, the recombinant nucleic acid molecule is an expression vector. In some embodiments, the recombinant nucleic acid molecule is a viral vector, such as, for example, a lentiviral vector. In some embodiments, the nucleotide sequence encoding E4ORF1 and / or BMP4 polypeptide is transiently expressed in the ntECs. In some embodiments, the nucleotide sequence encoding BMP4 and / or E4ORF1 polypeptide is stably expressed in the ntECs. In some embodiments, the nucleotide sequence encoding E4ORF1 and / or a BMP4 polypeptide is integrated into the genome of the ntECs.

[0013] In some embodiments, when a recombinant nucleic acid molecule comprising a nucleotide sequence encoding an E4ORF1 polypeptide is used, the recombinant nucleic acid molecule is not an adenovirus genome.In some embodiments, when a recombinant nucleic acid molecule comprising a nucleotide sequence encoding an E4ORF1 polypeptide is used, the recombinant nucleic acid molecule is not a naturally occurring adenovirus genome.In some embodiments, when a recombinant nucleic acid molecule comprising a nucleotide sequence encoding an E4ORF1 polypeptide is used, the recombinant nucleic acid molecule is not an adenovirus vector.

[0014] These and other embodiments of the invention are further described in other sections of this specification. Moreover, as will be appreciated by those skilled in the art, certain modifications and combinations of the various embodiments described herein fall within the scope of the present invention. [Brief description of the drawings]

[0015] [Figure 1] In vitro proliferation assay of four cell lines derived from the same umbilical cord. 500,000 cells of each cell line were seeded in small 25cm2 flasks on the first day. All proliferating cell lines were grown in the presence of serum. E4ORF1+ HUVEC and BMP4+ E4ORF1+HUVEC covered four flasks with a surface area of ​​225cm2 (4xT225) within 15 days, whereas HUVEC and BMP4+HUVEC only covered one T225 flask (1xT225) in 16 days. [Diagram 2] Figure 2A-C. BMP4+ E4ORF1+ntECs stimulate thymus regeneration. The results of the experiment are described in Example 2. Each graph shows the number of viable thymocytes (Figure 2.A), viable medullary epithelial cells (mTEC) (Figure 2B), and recovered viable cortical epithelial cells (cTEC) (Figure 2C) for the following treatment groups: (1) no Rad, no ECs; (2) no Rad, ECs; (3) Rad, Mu thymic ECs; (4) Rad Mu BMECs; and (5) Rad. Mu BMEC / BMP4. The y-axis shows the total number of cells isolated from the thymus of each individual animal. Significant differences between treatment groups are indicated by stars, where * indicates P value < 0.05, ** indicates P value < 0.01, and ** indicates P value < 0.001. Mu = murine (i.e., mouse). BMECs are bone marrow endothelial cells. Mu BMEC / BMP4 cells express both E4ORF1 and BMP4. [Diagram 3]Figure 3A-F. BMP4+E4ORF1+ntECs stimulate thymus regeneration. The results of the experiment are described in Example 2. Graphs showing the number of cells of a given type isolated from the thymus of each animal. Figure 3A - Total thymic epithelial content measured by CD45-EpCam+ cell count. Figure 3B - Viable and proliferating thymic epithelial cell content measured by CD45-EpCam+ Ki67+ cell count. Figure 3C - Thymic epithelial progenitor cell (TEPC) content measured by EpCam+ α6 integrin+Sca1+ cell count. Figure 3D - Actively proliferating TPEC content measured by EpCam+ α6 integrin+Sca1+Ki67+ cell count. Figure 3E - Thymic epithelial progenitor cell (TEPC) content measured by CD45-K5+K8+ cell count. Figure 3F - Actively proliferating TEPC content measured by CD45-K5+K8+Ki67+ cell count. In each case, data from two treatment groups are shown: a control without ECs ("650cGy No ECs") and a group treated with BMP4+E4ORF1+human umbilical vein endothelial cells (HUVECs) ("650cGy 500K AB245 cells") Significant differences between control and treatment groups are indicated by their P values. [Figure 4] Total thymocyte counts 3 weeks after transplantation. Total thymic cell content 3 weeks (3W) after lethal (100cGy) total body irradiation and cell transplantation. Co-injection of rescue mice bone marrow (bone marrow alone) with BMP4+E4ORF1+HUVEC (E4 / BMP4) tended to show a numerically more significant effect on thymocyte recovery than E4ORF1+HUVEC (E4), the parental non-BMP4 expressing mice. Statistical significance between groups was tested by Kruskal Wallis One Way ANOVA and Dunn's multiple comparison test when comparing bone marrow alone group with the other irradiated group, i.e., treated with human cells. [Diagram 5] Figure 5. Number of donor CD45+ cells in the thymus. For further explanation of the data in this figure, see Example 5. [Figure 6] Figure 6A-B. Recovery of CD3+ cells in the thymus. For further explanation of the data in this figure, see Example 5. [Figure 7]Figure 7A-B. Recovery of T lymphocytes expressing either CD4 or CD8. For further explanation of the data in this figure, see Example 5. [Figure 8] Figure 8A-B. Relative contribution of CD4+ and CD8+ cells to total CD3+ cell counts. For further explanation of the data in this figure, see Example 5. [Figure 9] Figure 9. Maturation of T-lymphocytes - Schematic representation. For further explanation of the information in this figure, see Example 5. [Figure 10] Figure 10A-B. Number (Figure 10A) and percentage (Figure 10B) of surviving CD3+ donor double positive (DP) T cells 3 weeks after 1000 cGY TBI. For further explanation of the data in this figure, see Example 5. [Figure 11] Number (FIG. 11A) and percentage (FIG. 11B) of surviving double negative (DN) donor CD45+ cells 3 weeks after 1000 cGY TBI. For further explanation of the data in this figure, see Example 5. [Figure 12] Figure 12A-D. Cell numbers of DN subpopulations in restored thymus. Figure 12A - DN1 cells. Figure 12B - DN2 cells. Figure 12C - DN3 cells. Figure 12D - DN4 cells. See Example 5 for further explanation of the data in this figure. [Figure 13] Figures 13A-D. Distribution of DN subpopulations in DN CD3 cells. Figure 13A - DN1 cells. Figure 13B - DN2 cells. Figure 13C - DN3 cells. Figure 13D - DN4 cells. For further explanation of the data in this figure, see Example 5. [Figure 14] Figure 14. Number of surviving CD45 negative / EpCam+ cells 3 weeks after 1000 cGY TBI. For further explanation of the data in this figure, see Example 5. [Figure 15] Figure 15. Recovery of EpCam+ / Sca1+ cells. For further explanation of the data in this figure, see Example 5. [Figure 16]Figure 16. Recovery of EpCam+ / Sca1+ / α6+ cells. For further explanation of the data in this figure, see Example 5. [Figure 17] Figure 17. Recovery of EpCam+ cortical TECs (cTECs). For further explanation of the data in this figure, see Example 5. [Figure 18] Figure 18. Restoration of proliferating (Ki67+) cTECs. For further explanation of the data in this figure, see Example 5. [Figure 19] Figure 19. Recovery of medullary TECs (mTECs). For further explanation of the data in this figure, see Example 5. [Figure 20] Figure 20. Recovery of expanded mTECs. See Example 5 for further explanation of the data in this figure. [Figure 21] Figure 21. BMP4+E4ORF1+ntECs improve survival after total body irradiation and bone marrow transplantation. Survival (y-axis) is plotted against time in days (x-axis) for a given treatment group. ECs are BMP4+E4ORF1+human umbilical vein endothelial cells (HUVECs). Animal survival 5 weeks after lethal total body irradiation is shown. Animals received 200,000 or 500,000 rescue mouse bone marrow (BM) with or without BMP4+E4ORF1+HUVECs. Detailed Description of the Invention

[0016] The "Summary of the Invention", "Figures", "Brief Description of the Drawings", "Examples" and "Claims" sections of this specification describe some of the main embodiments of the present invention. This "Detailed Description of the Invention" section provides specific additional description related to the compositions and methods of the present invention and is intended to be read in conjunction with all other sections of this specification. Moreover, as will be apparent to one of ordinary skill in the art, it is intended that the various embodiments described throughout this specification can be and are combined in various ways. Such combinations of the specific embodiments described herein are intended to fall within the scope of the present invention.

[0017] Specific definitions and abbreviations are provided below. Other terms or phrases may have meanings defined elsewhere in this specification or that are clear from the context in which they are used. Unless otherwise specified herein or unless otherwise clear from the context in this specification, all technical and scientific terms and abbreviations used herein have the same meanings as those commonly understood by ordinary skilled artisans in the technical field to which the present invention pertains. For example, The Dictionary of Cell and Molecular Biology (5th ed. JM Lackie ed., 2013), Oxford Dictionary of Biochemistry and Molecular Biology (2d ed. R. Cammack et al. eds., 2008) and The Concise Dictionary of Biomedicine and Molecular Biology (2d ed. PS. Juo, 2002) can provide a person skilled in the art with a general definition of some terms used herein.

[0018] As used in this specification and the appended claims, the singular articles include the plural counterparts unless expressly stated otherwise. The terms "a," "an," "one or more," and "at least one" can be used interchangeably.

[0019] Furthermore, "and / or" shall be considered to be specifically disclosed with respect to each of two specified features or components, whether or not the other is included. Thus, the term "and / or" used in a phrase such as "A and / or B" is intended to include A and B, A or B, A (alone) and B (alone). Similarly, the term "and / or" used in a phrase such as "A, B, and / or C" is intended to include A, B, and C; A, B, or C; A or B; A or C; B or C; A and B; A and C; B and C; A (alone); B (alone); and C (alone), etc.

[0020] Units, prefixes and symbols are expressed in the form accepted by the International System of Units (SI). Numerical ranges are inclusive of the numerical values ​​defining the range, and each value provided herein may serve as an end point of a range that includes other individual values ​​provided herein. For example, a collection of values ​​such as 1, 2, 3, 8, 9 and 10 is also a range of values ​​from 1 to 10.

[0021] Whenever an embodiment is described with the term "comprising," other similar embodiments that are described with the terms "consisting of" and / or "consisting essentially of" are included.

[0022] As used herein, the terms "about" and "approximately," when used in connection with a numerical value, the term "about" refers to the specified value ±10% standard deviation.

[0023] As used herein, the term "allogeneic" means obtained from, derived from, or members of the same species, where the members are genetically related or genetically similar but not genetically related. In embodiments involving administration of allogeneic ntECs to a subject, the allogeneic cells are obtained from a donor of the same species as the subject (i.e., recipient) to whom the cells are administered. In some embodiments, the allogeneic cells are obtained from a donor having the same MHC / HLA type as the subject (i.e., recipient) to whom the cells are administered, i.e., the donor of the cells and the recipient of the cells are MHC type-matched or HLA type-matched. In some embodiments, the cells (e.g., ntECs) are: (a) obtained from a donor, (b) maintained and / or cultured and / or expanded and / or genetically modified in vitro, and (c) then administered to a subject of the same species as the donor. For example, in some embodiments, the ntECs are obtained from a donor and are administered with BMP4 + and / or E4ORF1 + In some embodiments, ntECs are obtained from a donor and then genetically modified in vitro to become BMP4 + and / or E4ORF1 + These are then genetically modified in vitro to produce a donor-specific antibody, and then administered to a recipient subject of the same species and MHC / HLA type as the donor.

[0024] As used herein, the term "autologous" means obtained or derived from the same subject. In embodiments involving administration of autologous ntECs to a subject, the autologous ntECs are obtained from the subject to whom the ntECs are administered (i.e., the donor and recipient of the ntECs are the same individual). In some embodiments, cells (e.g., ntECs) are (a) obtained from a subject, (b) maintained and / or cultured and / or expanded and / or genetically modified in vitro, and (c) subsequently administered to the same subject. For example, in some such embodiments, the ntECs are obtained from a subject and are administered with BMP4. +and / or E4ORF1 + and then administered to the same subject.

[0025] As used herein, the abbreviation "BMP4" means bone morphogenetic protein 4 or the nucleotide sequence encoding that protein - if clear from the context of use.

[0026] As used herein, the abbreviation "EC(s)" means endothelial cells (plural). As used herein, the abbreviation "ntEC(s)" means non-thymic endothelial cells.

[0027] As used herein, the abbreviation "E4ORF1" means open reading frame (ORF) 1 of the early 4 (E4) region of the adenoviral genome, or the polypeptide / protein encoded by that ORF - if clear from the context of use.

[0028] As used herein, the term "culture" refers to the growth of cells on or in different types of media. "Co-culture" refers to the growth of two or more different types of cells on or in different types of media.

[0029] As used herein, the term "effective amount" refers to an amount of ntECs, or a therapeutic composition containing ntECs, sufficient to achieve a described therapeutic outcome when assessed using a detectable level - e.g., those described in the Examples section herein or methods for measuring therapeutic outcome. Therapeutic outcome is further described below (see definition of "treatment" - referring to various parameters / therapeutic outcomes). An appropriate "effective amount" in any individual case may be empirically determined using standard techniques known in the art, such as, for example, dose escalation studies, and may be determined taking into account factors such as the proposed route of administration, desired frequency of administration, and the like. Additionally, an "effective amount" may be determined using an assay such as those described in the Examples section herein. In some embodiments, an effective amount is about 5 x 10 per kg of subject body weight. 6 In some embodiments, the effective amount is about 1×10 ntECs per kg of the subject's body weight. 6 ~Approx. 50×10 6 In some embodiments, the effective amount is about 5×10 ntECs / kg of the subject's body weight. 6 ~Approx. 25×10 6 In some embodiments, the effective amount is about 5×10 ntECs / kg of the subject's body weight. 6 In some embodiments, the effective amount is about 10×10 ntECs per kg of the subject's body weight. 6 In some embodiments, the effective amount is about 15×10 ntECs / kg of the subject's body weight. 6 In some embodiments, the effective amount is about 20×10 ntECs per kg of the subject's body weight. 6 In some embodiments, the effective amount is about 25×10 ntECs / kg of the subject's body weight. 6 In some embodiments, the effective amount is about 30×10 ntECs / kg of the subject's body weight. 6 In some embodiments, the effective amount is about 35×10 ntECs / kg of the subject's body weight. 6 In some embodiments, the effective amount is about 40×10 ntECs per kg of the subject's body weight. 6In some embodiments, the effective amount is about 45×10 ntECs / kg of the subject's body weight. 6 In some embodiments, the effective amount is about 50×10 ntECs / kg of the subject's body weight. 6 ntECs.

[0030] The term "genetically engineered" as used in relation to non-thymic endothelial cells (ntECs) refers to ntECS cells that have been genetically engineered by humans to produce a referenced phenotype (e.g., E4ORF1 expression, BMP4 expression, or E4ORF1 and BMP4 expression) or that express a referenced nucleic acid molecule or polypeptide. The term "genetically engineered cells" is not intended to encompass naturally occurring cells, but instead is intended to encompass, for example, cells that contain recombinant nucleic acid molecules or cells that have been artificially altered in other ways (e.g., as described below). For example, it is intended to encompass cells that have been artificially altered (e.g., by genetic modification) to express a polypeptide that is not otherwise expressed or to express a polypeptide at a substantially higher level than observed in non-genetically engineered endothelial cells (e.g., to overexpress BMP4).

[0031] The terms "genetically modified" and / or "genetically modified" and / or "modified gene" refer to any addition, deletion, alteration or disruption to a nucleotide sequence or component of a cell's genome or genetic material of a cell. In some embodiments, the endothelial cells described herein may optionally include one or more additional genetic modifications in addition to being genetically modified to provide a nucleic acid molecule encoding E4ORF1 and / or a nucleic acid molecule encoding BMP4. The term "genetically modified" and related terms described above encompass both transient and stable genetic modifications and include the use of a wide variety of gene delivery means and methods, including, but not limited to, transduction (viral-mediated introduction of a nucleic acid into a recipient, either in vivo or in vitro), transfection (uptake by a cell of an isolated nucleic acid), liposome-mediated introduction and other means of gene delivery well known in the art.

[0032] As used herein, the term "isolated" refers to a cell population that is separated from at least one other cell population, product, compound or composition with which it is normally associated and / or that is not present within the body of a living subject.

[0033] As used herein, the term "recombinant" refers to a nucleic acid molecule that is isolated, produced and / or designed by man (including machine) using molecular biology and genetic engineering methods (such as molecular cloning) that contains a nucleotide sequence that does not occur in nature, is contained in a nucleotide sequence that does not occur in nature, is provided with a nucleotide sequence that is associated with it in nature, or is provided without a nucleotide sequence that is normally associated with it in nature. Thus, a recombinant nucleic acid molecule should be distinguished from a naturally occurring nucleic acid molecule, such as a nucleic acid molecule that occurs in the genome of an organism. For example, a nucleic acid molecule that contains a copy of a complementary DNA or "cDNA" mRNA sequence without an intervening intron sequence as found in the corresponding genomic DNA sequence would be considered a recombinant nucleic acid molecule. As a further example, a recombinant E4ORF1 nucleic acid molecule may contain an E4ORF1 coding sequence operably linked to a promoter and / or other genetic elements with which the coding sequence is not normally associated in the naturally occurring adenovirus genome. Similarly, a recombinant BMP4 nucleic acid molecule can comprise a BMP4 coding sequence operably linked to a promoter and / or other genetic elements with which the coding sequence is not normally associated in the genome of an organism.

[0034] The term "subject" includes mammals (e.g., humans and non-human primates), as well as other mammals (e.g., rabbits, rats, mice, cats, dogs, horses, cows, sheep, goats, pigs, etc.). In some embodiments, the subject is a mammalian subject. In some embodiments, the subject is a human. In some embodiments, the subject is a non-human primate.

[0035] The phrase "substantially pure" as used herein in reference to a cell population refers to a population of cells that are at least about 50%, preferably at least about 75%, more preferably at least about 85%, and most preferably at least about 95% of the cells that make up the total cell population, of a particular type (e.g., as determined by expression of one or more unique cell markers, morphological characteristics, or functional characteristics). Thus, a "substantially pure cell population" refers to a cell population that contains less than about 50%, preferably less than about 25%, more preferably less than about 15%, and most preferably less than about 5% of cells that are not of the specified type or species.

[0036] The terms "treat" and "regenerate" (and grammatical variations thereof) and related method terms (e.g., "treatment method" and "regenerative method"), as used herein in reference to the thymus, refer to detectably increasing or promoting (collectively "enhancing") any of the designated parameters or one or more of the following parameters (a)-(t), each of which is shown herein to be increased or promoted (i.e., enhanced) when the engineered ntECs of the invention are administered to a living subject: (a) total thymocytes (CD45 + Thymocytes and CD45 - (b) thymic mass; (c) production of self-restrictive and / or self-tolerant and / or immunodeficient and / or naive T cells; (d) thymic function (support of lymphoid cells such as T cells); (e) number or proliferation of T cell precursors, immature T cells or mature T cells; (f) CD45 + (g) CD3 cell count or proliferation + (h) CD3 cell count or proliferation + CD4 + Cell count or proliferation, (i) CD3 + CD8 + cell count or proliferation, (j) CD3 + CD4 + CD8 + (double positive or "DP" cell) count or proliferation, (k)CD4 - CD8 -(l) number or proliferation of thymic stromal cells; (m) number or proliferation of thymic epithelial cells; (n) number or proliferation of thymic CD45 - EpCam + Cell number or proliferation, (o) Thymic CD45 - EpCam + Number or proliferation of Sca1+ cells (TEPC), (p) thymic CD45 - EpCam + Cell number or proliferation (cTEC), (q) Thymic CD45 - EpCam + Ki67 + Cell number or proliferation (proliferated cTEC cells), (r) thymic CD45 - EpCam + (s) Thymic CD45 - EpCam + Ki67 +(number or proliferation of expanded mTEC cells) and (t) number or proliferation of one or more cell types shown in Table A (see below). In certain embodiments, a persistent or transient increase or promotion (collectively "enhancement") of one or more of these parameters / treatment outcomes indicates that the subject has been successfully "treated" or "regenerated" or "restored" with respect to the thymus or a particular thymic cell type or variety. In some embodiments, the detection and / or determination of the amount / level of enhancement (increase or promotion) of such parameters / treatment outcomes is assessed relative to a suitable baseline or a suitable control: for example, compared to the level of the parameter before the treatment method is initiated, or compared to the level of the parameter in a comparable control subject in which the method has not been performed, or compared to the level of the parameter when the method is performed in the absence of ntECS (e.g., using a delivery vehicle but not ntECs), or compared to the level of the parameter when the method is performed using non-genetically engineered ntECs (e.g., ntECs that do not express BMP4 and / or ntECs that do not express E4ORF1). In some embodiments, the amount of enhancement (increase or promotion) of one or more parameters / therapeutic outcomes may be any detectable amount. In some embodiments, the amount of enhancement (increase or promotion) of one or more parameters / therapeutic outcomes may be any statistically significant amount. In some embodiments, the amount of enhancement (increase or promotion) of one or more parameters may be about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 200%, 250%, 300%, 400%, 500%, or more compared to an appropriate baseline or an appropriate control. In some embodiments, the amount of enhancement (increase or promotion) of one or more parameters may be that the parameter / therapeutic outcome is at about a "normal" level for the subject, i.e., a level expected in a healthy individual, or a level expected in a subject without any disease, or a level that the subject has before developing a disease or before being treated with chemotherapy, radiation therapy, transplant conditioning regimen, or myeloablative conditioning regimen.In some embodiments, the amount of enhancement (increase or promotion) of one or more of the parameters may be such that the parameter is at a level higher than the "normal" level. In some embodiments, the amount of enhancement (increase or promotion) of one or more of the parameters may be such that the parameter is at about 50%, more preferably about 60%, more preferably about 70%, more preferably about 80%, or more preferably about 90% of the "normal" level. It is noted that for each embodiment of the invention describing a method of enhancing thymus regeneration, a corresponding method of increasing thymus regeneration and a corresponding method of promoting thymus regeneration are also contemplated. Similarly, it is noted that for each embodiment of the invention describing a method of enhancing thymus regeneration, a method of enhancing any one or more of the above specific parameters / therapeutic outcomes (e.g., parameters a-s) are also contemplated.

[0037] As used herein, the term "thymocytes" refers to cells that normally form part of or are present in the thymus, including, but not limited to, those set forth in Table A below. Within the general category of thymocytes are both thymic stromal cells and cells derived from the hematopoietic lineage (e.g., T cell precursors, immature T cells, and mature T cells, etc.) that are present in the thymus at least for a period of time, e.g., during the T cell maturation and T cell education processes that occur in the thymus. The term "thymic regeneration" (defined above) includes both the regeneration of thymic stromal cells and the regeneration of cells derived from the hematopoietic lineage, and also includes the term "T cell reconstitution." Effects on cells of hematopoietic origin that occur as part of the thymic regeneration process can be observed based on evaluation of cells of hematopoietic origin present in the thymus and / or based on evaluation of such cells in circulation - for example, enhanced T cell production from the thymus following administration of the engineered ntECs of the invention can be detected by evaluating such cells in circulation. [Table 1]

[0038] Nucleic Acid Molecules and Polypeptides Some of the embodiments of the invention described herein involve the use of E4ORF1 + , BMP4 + and / or E4ORF1 + BMP4 + The present invention also includes genetically engineered endothelial cells (ntECs) that are capable of expressing an E4ORF1 polypeptide, a BMP4 polypeptide, or both an E4ORF1 polypeptide and a BMP4 polypeptide. These polypeptides are collectively referred to herein as "polypeptides of the invention."

[0039] The "polypeptides of the invention" are encoded by nucleic acid molecules. Thus, in some embodiments, the invention includes nucleic acid molecules encoding adenoviral E4ORF1 polypeptides, nucleic acid molecules encoding BMP4 polypeptides, and / or nucleic acid molecules encoding both adenoviral E4ORF1 and BMP4 polypeptides. Collectively, such nucleic acid molecules are referred to herein as "nucleic acid molecules of the invention."

[0040] The polypeptides of the invention and the nucleic acid molecules of the invention may have an amino acid or nucleotide sequence as defined herein or known in the art, or may have an amino acid or nucleotide sequence that is a variant, derivative, mutant or fragment of said amino acid or nucleotide sequence - provided that such variant, derivative, mutant or fragment comprises or encodes a polypeptide having one or more functional properties as described herein (including, but not limited to, the ability to be expressed in ntECs and to induce thymus regeneration and / or T cell reconstitution when administered to a subject in need thereof).

[0041] In those embodiments that include a BMP4 polypeptide, the BMP4 polypeptide can be any mammalian BMP4 polypeptide, such as a human, non-human primate, rabbit, rat, mouse, goat or pig BMP4 polypeptide. In some embodiments, the polypeptide can be a human BMP4 polypeptide. The amino acid sequences of such polypeptides, and the nucleic acid sequences encoding the polypeptides, are well known in the art and available in well-known public databases, such as the Genbank database. For example, suitable human amino acid sequences of human BMP4 include those having the following accession numbers: NP_001193.2 GI:157276593, NP_570911.2 GI:157276595, NP_570912.2 GI:157276597, NP_001334841.1 GI:1122781626, NP_001334842.1 GI:1122781519, NP_001334843.1 GI:1122780734, NP_001334844.1 GI:1122781552, NP_001334845.1 GI:1122780682, and NP_001334846.1. GI: 1122781077. The human BMP4 polypeptide used in the experiments described in the Examples section herein was encoded by the following nucleotide sequence:

[0042] TIFF0007637418000002.tif145142

[0043] This nucleotide sequence encodes the following BMP4 amino acid sequence: TIFF0007637418000003.tif53145.

[0044] In those embodiments relating to adenovirus E4ORF1 polypeptides, the polypeptide sequence used may be from any suitable adenovirus type or strain, such as human adenovirus types 2, 3, 5, 7, 9, 11, 12, 14, 34, 35, 46, 50, or 52. In some preferred embodiments, the polypeptide sequence used is from human adenovirus type 5. The amino acid sequences of such adenovirus polypeptides, and the nucleic acid sequences encoding said polypeptides, are well known in the art and available in well-known publicly available databases, such as the Genbank database.For example, suitable sequences include human adenovirus type 9 (Genbank Accession No. CAI05991), human adenovirus type 7 (Genbank Accession No. AAR89977), human adenovirus type 46 (Genbank Accession No. AX70946), human adenovirus type 52 (Genbank Accession No. ABK35065), human adenovirus type 34 (Genbank Accession No. AAW33508), human adenovirus type 14 (Genbank Accession No. AAW33146), human adenovirus type 50 (Genbank Accession No. AAW33554), human adenovirus type 2 (Genbank Accession No. AP.sub.--000196), human adenovirus type 12 (Genbank Accession No. AP.sub.--000141), human adenovirus type 35 (Genbank Accession No. No. AP.sub.--000607), human adenovirus type 7 (Genbank Accession No. AP.sub.--000570), human adenovirus type 1 (Genbank Accession No. AP.sub.--000533), human adenovirus type 11 (Genbank Accession No. AP.sub.--000474), human adenovirus type 3 (Genbank Accession No. ABB 17792) and human adenovirus type 5 (Genbank Accession Number D12587).

[0045] In some embodiments, the polypeptides and nucleic acid molecules of the invention have the same amino acid or nucleotide sequences as those specifically described herein or known in the art (e.g., in a public sequence database such as the Genbank database). In some embodiments, the polypeptides and nucleic acid molecules of the invention may have amino acid or nucleotide sequences that are variants, derivatives, mutants or fragments of the sequences, e.g., variants, derivatives, mutants or fragments having 85% or more sequence identity to such sequences. In some embodiments, variants, derivatives, mutants or fragments have about 85% identity to a known sequence, or about 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to a known sequence. In some embodiments, variants, derivatives, mutants, or fragments of known nucleotide sequences that vary in length from the known nucleotide sequence by about 50, 45, 40, 35, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 nucleotide are used. In some embodiments, variants, derivatives, mutants, or fragments of known amino acid sequences that vary in length from the known amino acid sequence by about 50, 45, 40, 35, 30, 28, 26, or 24 amino acids are used. Variants, derivatives, mutants or fragments of the known amino acid sequence that differ in length from the known amino acid sequence by any of 22 amino acids, 20 amino acids, 18 amino acids, 16 amino acids, 14 amino acids, 12 amino acids, 10 amino acids, 9 amino acids, 8 amino acids, 7 amino acids, 6 amino acids, 5 amino acids, 4 amino acids, 3 amino acids, 2 amino acids or 1 amino acid are used.

[0046] In embodiments in which E4ORF1 nucleic acid or amino acid sequences are used, in some embodiments such sequences are used without any sequence apart from the adenoviral E4ORF1 region - e.g., not in the sense of the nucleic acid sequence of the complete E4ORF1 region or in conjunction with other polypeptides encoded by this E4ORF1 region. However, in some other embodiments such sequences may be used in conjunction with one or more other nucleic acid or amino acid sequences from the E4ORF1 region, such as E4ORF2, E4ORF3, E4ORF4 or E4ORF5 sequences, or variants, derivatives, mutants or fragments thereof. For example, E4ORF1 sequences can be used in constructs (e.g., viral vectors) that include other sequences, genes or coding regions (e.g., promoters, marker genes, antibiotic resistance genes, etc.), but in certain embodiments E4ORF1 sequences are used in constructs that do not include the entire E4ORF1 region or that do not include ORFs apart from the complete E4ORF1 region, such as E4ORF2, E4ORF3, E4ORF4 and / or E4ORF5.

[0047] The nucleic acid molecules of the invention can be used in constructs or vectors that contain, for example, promoters, enhancers, antibiotic resistance genes, reporter genes or expression tags (such as, for example, a nucleotide sequence encoding GFP), or any other nucleotide sequence or gene that may be desired, depending on the desired use. The polypeptides of the invention can be expressed alone or as part of a fusion protein.

[0048] In some embodiments, the nucleic acid molecule of the present invention may be under the control of one or more promoters to allow expression. Any promoter capable of driving the expression of the nucleic acid sequence in the desired cell type may be used. Examples of suitable promoters include, but are not limited to, CMV, SV40, RSV, HIV-Ltr and MML promoters. The promoter may also be a promoter from the adenovirus genome or a variant thereof. For example, in some embodiments in which E4ORF1 is used, the promoter may be the promoter that naturally drives the expression of E4ORF1 in the adenovirus genome. However, in other embodiments in which E4ORF1 is used, the promoter is not one that naturally drives the expression of E4ORF1 in the adenovirus genome.

[0049] In some embodiments, the nucleic acid molecules of the present invention can be placed under the control of an inducible promoter so that the expression of the nucleic acid sequence can be turned on or off as desired. Any suitable inducible expression system can be used, such as a tetracycline-inducible expression system or a hormone-inducible expression system. For example, the nucleic acid molecules of the present invention can be expressed for as long as they are needed and stopped when the desired result is achieved, such as when there is sufficient growth or proliferation of endothelial cells. The ability to turn expression on or off is believed to be particularly useful in in vivo applications.

[0050] The nucleic acid molecules of the present invention may comprise naturally occurring nucleotides, synthetic nucleotides or combinations thereof. For example, in some embodiments, the nucleic acid molecules of the present invention may comprise RNA, such as synthetic modified RNA, which is stable in cells and can be used to direct protein expression / production directly in cells. In other embodiments, the nucleic acid molecules of the present invention may comprise DNA. In embodiments in which DNA is used, the DNA sequence may be operably linked to one or more suitable promoters and / or regulatory elements to enable (and / or promote, enhance or regulate) expression in the cells and may be present in one or more suitable vectors or constructs. The nucleic acid molecules of the present invention may be introduced into endothelial cells in the same nucleic acid construct or in separate nucleic acid constructs.

[0051] The nucleic acid molecule of the present invention can be introduced into endothelial cells using any suitable system known in the art, including but not limited to transfection and virus-mediated transduction techniques.Transfection methods that can be used according to the present invention include but are not limited to liposome-mediated transfection, polybrene-mediated transfection, DEAE-dextran-mediated transfection, electroporation, calcium phosphate precipitation, microinjection and microparticle bombardment.Virus-mediated transduction methods that can be used include but are not limited to lentivirus-mediated transduction, adenovirus-mediated transduction, retrovirus-mediated transduction, adeno-associated virus-mediated transduction, herpes virus-mediated transduction, etc.

[0052] The present invention also provides vectors, such as expression vectors, that contain the nucleic acid molecules of the present invention.For example, in one embodiment, the present invention provides expression vectors that include nucleotide sequences that code for BMP4 and / or E4ORF1 polypeptides.In some such embodiments, the expression vector is a viral vector.In some such embodiments, the expression vector is a lentiviral vector.In some embodiments, the nucleotide sequence that codes for BMP4 and the nucleotide sequence that codes for E4ORF1 are provided in the same construct or vector and can be under the control of separate promoters, or can be under the control of another promoter, for example, that includes an internal ribosome entry site sequence (IRES) between the BMP4 and E4ORF1 sequences.

[0053] In some embodiments, peptide mimetics may be used.Peptide mimetics are small molecule protein-like chains designed to mimic polypeptides.Such molecules can be designed to mimic any of the polypeptides of the present invention (e.g., BMP4 or E4ORF1 polypeptides).Many different methods of modifying peptides to make peptide mimetics or designing peptide mimetics are known in the art and can be used to make a peptide mimetic of one of the polypeptides of the present invention.

[0054] The manipulation, engineering and expression of the polypeptides and nucleic acid molecules of the present invention can be carried out using conventional molecular biology and cell biology techniques. Such techniques are well known in the art. For example, Sambrook, Fritsch and Maniatis eds., "Molecular Cloning A Laboratory Manual, 2nd Ed., Cold Springs Harbor Laboratory Press, 1989); the series Methods of Enzymology (Academic Press, Inc.) or any other standard reference textbook teachings can be consulted for guidance on suitable techniques to use in the manipulation, engineering and expression of nucleotide and / or amino acid sequences. Further aspects related to the manipulation or expression of E4ORF1 sequences are described in U.S. Pat. No. 8,465,732, the contents of which are incorporated herein by reference.

[0055] endothelial cells The non-thymic endothelial cells (ntECs) described herein can be any suitable non-thymic origin of vascular endothelial cells known in the art. In some embodiments, the ntECs do not express one or more markers (or marker profile) that are specific for thymic endothelial cells. In some embodiments, the endothelial cells are primary endothelial cells. In some embodiments, the endothelial cells are mammalian cells, such as human or non-human primate cells, or rabbit, rat, mouse, goat, pig, or other mammalian cells. In some embodiments, the endothelial cells are primary human endothelial cells. In some embodiments, the endothelial cells are umbilical vein endothelial cells (UVECs), such as human umbilical vein endothelial cells (HUVECs). In some embodiments, the endothelial cells are adipose ECs. In some embodiments, the endothelial cells are skin ECs. In some embodiments, the endothelial cells are myocardial ECs. In some embodiments, the endothelial cells are kidney ECs. In some embodiments, the endothelial cells are lung ECs. In some embodiments, the endothelial cells are liver ECs. In some embodiments, the endothelial cells are bone marrow ECs. Other suitable endothelial cells that can be used include those previously described as suitable for E4ORF1-expression, as described in U.S. Patent No. 8,465,732, the contents of which are incorporated herein by reference.

[0056] In some embodiments, the endothelial cells are genetically modified to comprise one or more genetic modifications in addition to and apart from expressing a molecule of interest (e.g., BMP4 and / or E4ORF1). For example, in some embodiments, the endothelial cells can also be engineered to express ETV2. Indeed, in each of the embodiments described herein in which the ntECs express BMP4 and / or E4ORF1, the ntECs can also express ETV2. Furthermore, in some embodiments, the ntECs described herein contain altered versions of genes known or suspected to be involved in a disease or disorder affecting endothelial cells or other genes, e.g., therapeutically useful genes, which one would wish to provide in the endothelial cells or administer or deliver using genetically engineered endothelial cells.

[0057] Cell populations and compositions The endothelial cells of the present invention may be present or provided in the form of an endothelial cell population or a composition comprising such an endothelial cell population.

[0058] For example, in one embodiment, the present invention provides a genetically engineered BMP4 + E4ORF1 +A population of non-thymic endothelial cells (ntECs) is provided. In some embodiments, such a population of genetically engineered ntECs exists in vitro or ex vivo. In some embodiments, such a population of genetically engineered ntECs exists in vivo. In some embodiments, such a population of genetically engineered ntECs is an isolated population. In some embodiments, such a population is a substantially pure population of ntECs cells. For example, in some embodiments, at least about 50%, preferably at least about 75%, more preferably at least about 85%, and most preferably at least about 95% of the cells constituting the total cell population will be genetically engineered ntECs of the present invention. In some embodiments, the ntECs in the genetically engineered ntEC population are umbilical vein endothelial cells (UVECs), adipose endothelial cells, skin endothelial cells, lung endothelial cells, cardiac endothelial cells, kidney endothelial cells, or bone marrow endothelial cells. In some embodiments, the ntECs are human umbilical vein endothelial cells (HUVECs).

[0059] In some embodiments, the present invention provides various compositions comprising a population of ntECs as described above or elsewhere herein. In some embodiments, such compositions include a carrier solution, such as saline, a cell suspension, or a cell culture medium. In some embodiments, such compositions are therapeutic compositions comprising a population of ntECs as described herein and a solution suitable for administration to a subject, such as saline. Other therapeutically acceptable agents may be included, if desired. One of skill in the art can readily select appropriate agents to be included in the therapeutic composition depending on the intended use. In some embodiments, such compositions and therapeutic compositions may include a population of ntECs as described herein and a biocompatible matrix material (e.g., a biocompatible matrix material that is liquid or solid at room or body temperature).

[0060] In some embodiments, the compositions described herein may include additional cell types, such as additional cell types that can be maintained, cultured or expanded in the presence of ntECs (e.g., using ntECs as "feeder" cells) or administered to a subject together with ntECs. Examples of such additional cell types include, but are not limited to, stem or progenitor cells, such as thymic stem or progenitor cells, hematopoietic cells, hematopoietic stem cells (HSCs), hematopoietic progenitor cells (HPCs), and hematopoietic stem and progenitor cells (HSPCs). Other examples of cells that can be provided or used together with the endothelial cells of the invention are provided in U.S. Patent No. 8,465,732, the contents of which are incorporated herein by reference.

[0061] Treatment methods and other applications In some embodiments, the present invention provides various therapeutic methods, e.g., methods of treating a subject in need thereof by administering to the subject an effective amount of the engineered ntECs of the present invention (or a composition comprising the engineered ntECs).

[0062] For example, in one embodiment, the present invention provides a method of enhancing thymic regeneration, comprising administering to a subject in need of thymic regeneration genetically engineered non-thymic endothelial cells (ntECs), wherein the genetically engineered ntECs express E4ORF1 + or BMP4 + E4ORF1 + The method is characterized in that the present invention comprises administering to a subject an effective amount of a therapeutic composition comprising:

[0063] In another embodiment, the present invention provides a method for enhancing survival after myeloablative conditioning in a subject, the method comprising administering to the subject who has undergone myeloablative conditioning an effective amount of a therapeutic composition comprising genetically engineered non-thymic endothelial cells (ntECs), the genetically engineered ntECs expressing BMP4. + E4ORF1 +Similarly, in another embodiment, the present invention provides a method for increasing survival rate after myeloablative conditioning and subsequent hematopoietic cell transplantation (HCT) (e.g., hematopoietic stem cell transplantation (HSCT)) in a subject, the method comprising administering to a subject undergoing myeloablative conditioning and subsequent hematopoietic cell transplantation (HCT) (e.g., hematopoietic stem cell transplantation (HSCT)) an effective amount of a therapeutic composition comprising engineered non-thymic endothelial cells (ntECs), the engineered ntECs being BMP4 + E4ORF1 + In such methods, survival is extended as compared to survival of a subject undergoing the same myeloablative conditioning treatment (and / or HCT) but not receiving the genetically engineered ntEC.

[0064] Means for selecting an effective amount of an appropriate ntECS, or composition containing ntECs, are as described above.

[0065] The genetically engineered non-thymic endothelial cells (ntECs) can be administered to the subject once (single dose) or multiple times (multiple doses), for example, 2, 3, or 4 times. When multiple doses are used, the schedule of administration can be any suitable schedule. In some embodiments, the multiple doses are 1 day apart, 2 days apart, 3 days apart, or 5 days apart. For example, in one embodiment, the ntECs are administered on days 0, 3, and 5. The appropriate time to initially administer the ntECs to the subject (i.e., day 0) can be selected by a physician based on the subject's particular condition. For example, in one embodiment, if the subject has undergone myeloablative conditioning in preparation for undergoing hematopoietic cell transplantation (HCT), the ntECs are initially administered to the subject on the same day the subject undergoes HCT (day 0), and the ntECs may or may not be administered again to the subject on subsequent days, for example, days 3 and 5 after HCT. In some embodiments, an effective amount of the ntECs is administered to the subject in one dose-single dose. In some embodiments, an effective amount of ntECs is administered to a subject multiple times, i.e., multiple times, each delivering an effective amount of ntECs to the subject. In some embodiments, an effective amount of ntECs is divided between multiple administrations, e.g., half of an effective amount is administered on the first day in a first administration, and half of an effective amount is administered on another day in a second administration. Many variations of these administration schemes can be employed as appropriate. Those skilled in the art will be able to select an appropriate administration schedule depending on the particular condition.

[0066] In some embodiments, thymic regeneration refers to the expression of CD45 - Thymic stromal cells and CD45 + This includes recovery of at least one cell type among T cells. CD45 - Thymic stromal cells include, but are not limited to, thymic epithelial progenitor cells (TEPCs), cortical thymic epithelial cells (cTECs) and medullary thymic epithelial cells (mTECs). + T cells have CD3 + T cells, CD4 + T cells, CD8 +These include, but are not limited to, double positive T cells (DP), double negative T cells (DN), double negative type 1 (DN1) T cells, double negative type 2 (DN2) T cells, double negative type 3 (DN3) T cells and double negative type 4 (DN4) T cells. In some embodiments, thymic regeneration is achieved by the expression of CD45 - Thymic stromal cells and CD45 + In some embodiments, thymic regeneration includes the recovery of both thymic epithelial progenitor cells (TEPCs), thymic cortical epithelial cells (cTECs), and medullary thymic epithelial cells (mTECs). In some embodiments, thymic regeneration includes the recovery of CD4+ T cells, CD8+ T cells, double positive T cells (DP), double negative T cells (DN), double negative type 1 (DN1) T cells, double negative type 2 (DN2) T cells, and double negative type 4 (DN4) T cells. In some embodiments, thymic regeneration includes the recovery of thymic epithelial progenitor cells (TEPCs), thymic cortical epithelial cells (cTECs), and medullary thymic epithelial cells (mTECs). CD4 + T cells, CD8 + This includes recovery of T cells, double positive T cells (DP), double negative T cells (DN), double negative type 1 (DN1) T cells, double negative type 2 (DN2) T cells, and double negative type 4 (DN4) T cells.

[0067] In some embodiments, the ntECs are selected from the group consisting of umbilical vein endothelial cells (UVECs), adipose endothelial cells, skin endothelial cells, lung endothelial cells, cardiac endothelial cells, kidney endothelial cells and bone marrow endothelial cells.

[0068] In some embodiments, the subject is a human. In some such embodiments, the ntECs are human umbilical vein endothelial cells (HUVECs). In some such embodiments, the ntECs are human bone marrow endothelial cells. In some such embodiments, the ntECs are human adipose endothelial cells. In some such embodiments, the ntECs are human dermal endothelial cells.

[0069] In some embodiments, the ntECs are autologous to the subject. In some embodiments, the ntECs are allogeneic to the subject. In some embodiments, the ntECs are MHC / HLA-matched to the subject.

[0070] In some embodiments, the subject has previously been treated with chemotherapy, radiation therapy, a transplant conditioning regimen, or a myeloablative conditioning regimen. Examples of myeloablative conditioning regimens include, but are not limited to, treating the subject with radiation (e.g., total body irradiation) and / or administering etoposide and / or busulfan to the subject.

[0071] In some embodiments, the subject has been previously treated with hematopoietic cell transplantation (HCT), hematopoietic stem cell transplantation (HSCT) or hematopoietic stem and / or progenitor cell transplantation (HSPCT).

[0072] In some embodiments, the subject has an immunodeficiency. In some embodiments, the subject has an HIV infection. In some embodiments, the subject has Age-related in thymic tissue mass, thymic function, or T cell production Lack of There is a shortage.

[0073] In some embodiments, the method further comprises administering to the subject a therapeutic composition comprising hematopoietic cells or hematopoietic stem cells (HSCs), or hematopoietic stem and progenitor cells (HSPCs) (e.g., in the context of performing a hematopoietic stem cell transplant (HSCT) or hematopoietic stem and / or progenitor cell transplant (HSPCT) procedure). In some such embodiments, the engineered ntECs are administered to the subject by intravenous (IV) infusion. In some such embodiments, the engineered ntECs and hematopoietic cells (e.g., HSCs) are administered simultaneously. In some such embodiments, the engineered ntECs and hematopoietic (e.g., hematopoietic stem cells) are administered to the subject in the same injection. In some such embodiments, the engineered ntECs are administered to the subject in multiple intravenous injections over the course of several days or weeks.

[0074] In some embodiments of the therapeutic methods provided herein, a BMP4 protein is administered to a subject. For example, in some such embodiments, the BMP4 protein is administered to a subject by immunizing the subject with E4ORF1 or E5ORF2 prior to administering such a composition to the subject. + or BMP4 + E4ORF1 + In some embodiments, a separate composition comprising a BMP4 protein is administered to the subject as part of the treatment method. In some embodiments of the treatment methods provided herein, BMP4 is not administered to the subject.

[0075] In some embodiments, thymic endothelial cells are also administered to the subject (i.e., both non-thymic ECs (ntECs) and thymic ECs are administered). In some embodiments, thymic endothelial cells are not administered to the subject.

[0076] In the therapeutic methods provided herein, the ntECs or compositions containing ntECs can be administered to a subject using any suitable means known in the art, for example, by injection (e.g., intravenous (IV) injection, intramuscular injection, subcutaneous injection, local injection), or by infusion (e.g., intravenous infusion, subcutaneous infusion, local injection), or by surgical implantation. In some embodiments, the ntECs or compositions containing ntECs are administered to a subject by IV infusion. For example, in some embodiments, the engineered ntECs of the invention may be administered directly into or near the thymus. In some embodiments, the engineered ntECs of the invention may be administered to a subject by intrathymic injection or infusion. In some embodiments, the engineered ntECs of the invention may be administered to a subject by injection or infusion into the inferior thyroid artery. In some embodiments, the engineered ntECs of the invention may be administered to a subject by injection or infusion into the internal thoracic artery. One of skill in the art will be able to select an appropriate administration route depending on the particular condition.

[0077] In some embodiments, the thymus may be rendered more permissive to homing of engineered ntECs to the thymus via mechanical, magnetic, ultrasonic or other stimulation methods.

[0078] In some embodiments, the engineered ntECs of the invention can be generated in vivo, e.g., for research purposes or therapeutic applications. For example, in some aspects, the invention provides various methods of treatment, e.g., methods for treating a subject in need of treatment, comprising administering to the subject an effective amount of a nucleic acid molecule encoding BMP4 and / or a nucleic acid molecule encoding E4ORF1 (e.g., in a suitable vector and / or under the control of a suitable promoter), by transfecting non-thymic endothelial cells of the subject with such nucleic acid molecules, or TransductionThe method includes administering the nucleotide molecule to a subject by a suitable means known to those skilled in the art. For example, the nucleotide molecule (e.g., in a suitable vector) can be administered by injection or infusion into the bloodstream or tissue at the desired site. The nucleic acid molecule can be administered in a single dose or multiple doses. Depending on the desired use, those skilled in the art will be able to select the appropriate administration method and the appropriate administration regime accordingly.

[0079] In some embodiments, the engineered ntECs of the present invention are mitotically inactivated prior to use (e.g., therapeutic use) so that they cannot replicate. This can be accomplished, for example, with chemical agents such as mitomycin C or by irradiating the engineered endothelial cells.

[0080] In some embodiments, the therapeutic methods of the invention involve genetically modifying ntECs in vitro or ex vivo by transduction or transfection with a nucleic acid molecule encoding E4ORF1 and, optionally, a nucleic acid molecule encoding BMP4 prior to administering the ntECs to a subject. Starting Step Further includes:

[0081] Any of the various therapeutic methods described herein can be used to enhance thymic regeneration (including stimulating T cell reconstitution) and / or achieve any one or more of the parameters or therapeutic outcomes listed in the definition of "Treatment" section above in a living subject in need thereof.

[0082] Cell culture method Methods for culturing cells are well known in the art, and any suitable cell culture method can be used. For example, the genetically engineered ntECs of the present invention can be cultured using methods known to be useful for culturing other endothelial cells, or methods known to be useful for culturing E4ORF1-expressing endothelial cells, such as those described in U.S. Pat. No. 8,465,732, the contents of which are incorporated herein by reference. In some embodiments, the genetically engineered endothelial cells of the present invention can be cultured in the absence of serum, or in the absence of exogenous growth factors, or in the absence of both serum and exogenous growth factors. The genetically engineered endothelial cells of the present invention can also be cryopreserved. Various methods for cell culture and cell cryopreservation are known to those skilled in the art, including, for example, those described in Culture of Animal Cells: A Manual of Basic Technique, 4th Edition (2000) by R. Ian Freshney ("Freshney"), the contents of which are incorporated herein by reference.

[0083] kit The present invention also provides kits for carrying out the various methods described herein or for producing the genetically engineered endothelial cells provided herein. Such kits can include any of the components described herein, including nucleotide sequences (e.g., in vectors), ntECs, populations of genetically engineered ntECS, control non-genetically engineered ntECs, sample / standard genetically engineered ntECs, means or compositions for detecting genetically engineered ntECs or proteins or nucleic acid molecules expressed therein (e.g., nucleic acid probes, antibodies, etc.), media or compositions useful for maintaining or growing genetically engineered ntECs, media conditioned by genetically engineered ntECs, means or compositions for administering genetically engineered endothelial cells to a subject, or any combination thereof. All such kits may optionally include instructions for use, containers, culture vessels, etc. A label may be attached to the kit or may include any written or recorded material, which may be in electronic or computer readable form (e.g., disk, optical disk, memory chip, or tape) that provides instructions or other information regarding the use of the contents of the kit.

[0084] Certain aspects of the present invention can be further illustrated in the following non-limiting examples. EXAMPLES

[0085] Example 1 Generation of genetically engineered non-thymic endothelial cells Isolation of non-thymic endothelial cells Non-thymic endothelial cells (ntECs) are isolated from a desired tissue, such as umbilical cord / vein, adipose tissue, skin, lung, heart, kidney or bone marrow, or any other desired non-thymic tissue source. Endothelial cells are isolated from the desired tissue using standard established protocols. An example protocol for isolating ntECs from umbilical cord is provided below. Similar protocols are known and can be used to isolate endothelial cells from other tissues.

[0086] Below is an illustrative protocol for isolating ntECS from umbilical veins from any desired species, such as humans. This protocol generates a population of umbilical vein endothelial cells or "UVECs" - called "HUVECs" when obtained from human umbilical veins. All steps are performed using aseptic technique and sterile materials.

[0087] The umbilical cord is maintained at 2-8°C until it is processed to isolate endothelial cells. UVECs are isolated from the umbilical vein by enzymatic digestion with collagenase. For example, the umbilical vein can be washed with saline or other solution suitable for viable cells (e.g., culture medium), filled with a solution of 0.2% (w / v) collagenase / saline or other solution suitable for viable cells (e.g., culture medium), and fixed with clamps at both ends. The umbilical cord is incubated at an appropriate temperature (e.g., room temperature 20-25°C) for a time sufficient for the endothelial cells to detach from the umbilical vein (e.g., about 30 minutes at room temperature). The time may vary depending on the temperature at which the incubation is performed and based on the specific properties of the tissue. The detached cells are washed out of the vein and stored in a suitable container, such as a conical tube. The umbilical vein can be washed again with saline or other solution suitable for viable cells (e.g., culture medium), and the washings can also be stored, for example, in the same container. The contents of the container are centrifuged and the supernatant aspirated. The cell pellet containing UVECs is gently resuspended in EC culture medium. An example of a culture medium that can be used is EC culture medium containing 10% fetal bovine serum, 20 ng / mL FGF-2, 10 U / mL heparin, 5 μg / mL gentamicin, 10 mM HEPES and 1X Glutamax supplemented with M199 basal medium. The UVECs in EC culture medium are transferred to a suitable tissue culture vessel and placed in an incubator at 37° C. and 5% CO2 to begin the in vitro culture process. Those skilled in the art will understand that modifications of this protocol can be made to achieve the isolation of UVECs. Those skilled in the art will also recognize that modifications of this protocol can be made to isolate ntECs from other tissues.

[0088] Transfection or transduction to generate genetically engineered ntECs After a suitable culture period (e.g., 2 days), ntECs isolated as described above, or isolated or obtained by any other means, are transfected or transduced (e.g., in an expression vector, viral vector, etc.) with a nucleic acid molecule comprising a selectable marker and a coding sequence(s) of interest (e.g., an E4ORF1 coding sequence (e.g., from Ad5) or a BMP4 coding sequence) under control of a suitable promoter. Transfection or transduction is performed using standard transfection or transduction protocols. If a second coding sequence is to be transfected or transduced (e.g., an E4ORF1 coding sequence (e.g., from Ad5) or a BMP4 coding sequence), after a suitable period (e.g., the next day), the ntECs are transfected or transduced with a second nucleic acid molecule comprising a selectable marker and a second coding sequence (e.g., in an expression vector, viral vector, etc.) under control of a suitable promoter, again using standard transfection or transduction protocols. Hato If two transfections / transductions are performed, the order of transfections / transductions can be in any desired order (e.g., E4ORF1 first or second). The two coding sequences can also be delivered simultaneously, whether in the same nucleic acid molecule or in separate nucleic acid molecules.

[0089] Typically, after transfection or transduction, the ntECs cells are maintained in EC medium for several days (e.g., 3 days) and then switched to an appropriate selection medium containing the appropriate selection agent or drug. For example, if the delivered nucleic acid molecule contains a gene that confers resistance to a given antibiotic, an appropriate amount of that antibiotic is provided in the selection medium - such that cells that do not contain the nucleic acid molecule will die and cells that contain the nucleic acid molecule will survive.

[0090] Typically, ntECs are subjected to serum starvation culture (E4ORF1 expression endows ntECs with the ability to survive in serum-free culture) for a period of time. The ntECs are then cultured in the appropriate EC medium using standard EC culture protocols for a sufficient time to generate sufficient numbers of cells for the desired application. For example, for UVECs, approximately 200-300x10 cells can be produced from a single umbilical cord over a 14-21 day proliferation period. 6 E4ORF1 + UVECs can be easily obtained. The amount of starting material and the growth period can be adjusted accordingly to obtain a desired amount of ntECs. If necessary, a bioreactor system can be used to produce large amounts of ntECs under controlled conditions. For example, the Quantum Cell Expansion System (Quantum, Terumo BCT) bioreactor system can be used. Those skilled in the art can easily obtain a population of genetically engineered ntECs (e.g., E4ORF1) suitable for a desired application. + , BMP4 + or BMP4 + E4ORF1 + It will be appreciated that modifications can be made to the above protocol to generate ntECs.

[0091] Freeze-drying of genetically engineered ntECs The genetically engineered ntECs produced as described above or by other means can be used immediately or maintained in culture until use, although in some circumstances it may be desirable to cryopreserve the ntECS and / or generate a ntEC cell bank, e.g., to allow the ntECs to be used at a later date. A variety of cryopreservation protocols and cryopreservation reagents suitable for use with endothelial cells are known in the art, and any such method / reagent may be used. For example, the genetically engineered ntECs may be cryopreserved using CryoStor CS5 freeze-drying reagent (BioLife Solutions, Seattle, Washington), optionally with human serum albumin (HSA: Griffols) added to a final concentration of about 20% HSA. The cryopreserved ntECs may be cryopreserved until needed. Typically, when needed, the ntECs are thawed, transferred to EC culture medium, and cultured and expanded to the desired extent.

[0092] Quality control of engineered ntECs Quality control testing is performed at any point during the production process. Steps And / or prior to use, genetically engineered ntECs can be performed as necessary. For example, quality control assays can be performed to evaluate and confirm cell viability, the presence or absence of contamination, the presence of BMP4 expression (e.g., by RT PCR), the presence of BMP4 secretion (e.g., by ELISA), the presence of E4ORF1 expression (e.g., by RT PCR), etc. For example, the evaluation of quality control data was based on detecting secreted BMP4 protein detected by ELISA. BMP4 + E4ORF1 + Conditioned medium was collected from wells containing HUVECs. BMP4 was detected in the conditioned medium at an average concentration of approximately 60,000 (n=2 independent lots, performed in duplicate). + E4ORF1 +In a separate quality control evaluation performed on the HUVECs lines, the average concentration of BMP4 in the conditioned medium was approximately 29,104 pg / mL for the first line, 4,542 pg / mL for the second line, 1,798 pg / mL for the third line, and 3,211 pg / mL for the fourth line.

[0093] Example 2 Resistance of non-thymic endothelial cells to serum starvation. Human umbilical vein endothelial cells were isolated and cultured in complete medium (medium supplemented with 20% fetal bovine serum and fibroblast growth factor-2; FGF-2) until confluent. The cells were then split into six wells, each containing 100,000 endothelial cells in complete medium. Cells in wells 1 and 2 were used as controls (not transfected); cells in wells 3 and 4 were transfected with a retrovirus carrying the BMP4 gene. Cells in wells 5 and 6 were transfected with a retrovirus carrying the E4ORF1 region of adenovirus, which has been shown to confer resistance to serum starvation.

[0094] All cells in wells 2, 4 and 6 were then switched to serum-free medium for four days; wells 1, 3 and 5 were maintained in complete medium for four days. At the end of the four days, each well was examined to determine cell proliferation. The results are summarized in Table 1 below.

[0095] [Table 2]

[0096] As a result, in the absence of gene transduction, the cells did not proliferate upon serum starvation (wells 1 and 2). Gene transduction with BMP4 did not confer resistance to serum starvation (wells 3 and 4), but gene transduction with adenovirus E4ORF1 conferred resistance (wells 5 and 6).

[0097] The results of these studies are further summarized in Table 2 below, which shows the effect (in relative comparison) of transfecting HUVECs with E4ORF1 alone, BMP4 alone, or both E4ORF1 and BMP4 on the ability of HUVECs to grow in the absence of serum. The "-" and "+" symbols are a measure of cell number, with more "+" symbols representing higher cell numbers.

[0098] [Table 3]

[0099] Example 3 Proliferation of non-thymic endothelial cells in the presence of serum. In this study, human umbilical vein endothelial cells were isolated and cultured in complete medium (medium supplemented with 20% fetal bovine serum and fibroblast growth factor-2; FGF-2) until confluent. The cells were then split into four wells, and each well contained 100,000 endothelial cells with complete medium. The cells in well 1 were used as a control (untransfected), the cells in well 2 were transfected with a retrovirus carrying the BMP4 gene, and the cells in well 3 were transfected with a retrovirus carrying the adenovirus E4ORF1 region, which is known to confer a growth advantage to the cells.

[0100] All cells were grown to confluence, then passaged and replated at 150,000 cells per well. All cultures were passaged every 4 to 6 days for 15 days. Cell numbers were determined at each passage using trypan blue exclusion and a hemocytometer.

[0101] The results showed that cells transduced with BMP4 alone did not proliferate faster than non-transduced cells, whereas cells transduced with E4ORF1 proliferated significantly faster (see FIG. 1). Thus, transduction of human umbilical vein endothelial cells with BMP4 did not confer a substantial increase in the proliferation rate to the cells. On the other hand, transduction with the adenovirus E4ORF1 region resulted in a substantial proliferation enhancement. These test results are further summarized in Table 3 below, which shows the effect (in the form of relative quantification) of transfection with E4ORF1 alone, BMP4 alone, or both E4ORF1 and BMP4 on the proliferation capacity of HUVECs cultured in the presence of serum. The "+" symbol is a measure of cell number, with more "+" symbols representing a higher cell number. For actual cell numbers, please refer to the figures showing the corresponding data.

[0102] [Table 4]

[0103] Example 4 BMP4 + E4ORF1 + Non-Thymic Endothelial Cells Stimulate Thymic Epithelial Cell Renewal In Vivo BMP4 + E4ORF1 + ntECs were generated essentially as described in the previous examples. C57Bl / 6 mice were exposed to 650 Rads of total body irradiation (TBI), a sublethal dose sufficient to induce systemic hematopoietic and thymic damage. Mice were injected with 1x10 6 Mouse thymic endothelial cells (mutECs) or BMP4 + E4ORF1 +Bone marrow endothelial cells (shown as "muBMECs+BMP4" in Figure 2) were suspended in saline (phosphate buffered saline - "PBS") and injected 6 hours after irradiation. There was also a "no ECs" control group injected with PBS only. Tissues were harvested on day 9 after TBI. The numbers of viable thymocytes, viable medullary thymic epithelial cells (mTECs), and recovered viable cortical thymic epithelial cells (cTECs) were determined. The results are shown in Figure 2, where the numbers of viable thymocytes (Figure 2A), viable medullary epithelial cells (mTECs) (Figure 2B), and recovered viable cortical epithelial cells (cTECs) (Figure 2C) are plotted on each graph. From this data, it was found that murine BMP4 + E4ORF1 + Bone marrow endothelial cells have been shown to promote thymic recovery after sublethal doses of radiation.

[0104] To distinguish the role of different subsets of thymic epithelial cells (TECs), namely mTECs and cTECs, in thymic regeneration, additional studies were performed to evaluate the role of BMP4 + E4ORF1 + We identified the cell type affected by ntEC treatment (shown as "AB245" cells in Figure 3). C57Bl / 6 mice were subjected to 650 Rads of TBI to induce thymic injury. Control irradiated mice were treated with BMP4 on days 1 and 3 post-irradiation. + E4ORF1 + The mice were then compared with those that had received HUVECs. Four days after irradiation, the animals were sacrificed and treated with BMP4. + E4ORF1 + We observed the effect of HUVEC transplantation. At this early time point, transplanted animals had almost double the amount of total thymic epithelium (CD45 - EpCam + These actively proliferating cells (CD45 - EpCam + Ki67 + The total number of EpCam cells increased by nearly four-fold (Figure 3B). + , thymic epithelial progenitor cells (TEPCs) identified by markers of α6 integrin+, Sca1+ (Figure 3C) and CD45 - , K5 +, K8 + The number of TEPCs (Figure 3E) was significantly increased compared to animals that did not receive cell transplantation. The proliferative population of these TEPCs was also increased, and BMP4 + E4ORF1 + The number of cells was found to be two to four times higher in animals that received HUVECs compared with animals that did not receive cell transplants ( Fig. 3, D and F ).

[0105] The results of the above studies are further summarized in Tables 4A and 4B below, which show the ability (in relative comparison) of the indicated ntEC types to promote recovery of the listed thymic cell type(s) in the thymus following sublethal irradiation (see column "Treatment Outcome"). The data summarized in Table 4A (data from Figure 2) show that BMP4 + E4ORF1 + The data summarized in Table 4B (data from FIG. 3) summarize the recovery induced by administration of bone marrow ECs. + E4ORF1 + Figure 1 summarizes the recovery induced by administration of HUVECs. The "+" symbol is a measure of cell number, with more "+" symbols representing higher cell numbers. For actual cell numbers, please refer to the corresponding data figures.

[0106] [Table 5] [Table 6]

[0107] Example 5 Genetically engineered non-thymic endothelial cells stimulate the recovery of both thymic T cells and thymic epithelial cells We investigated the ability of engineered ntECs to stimulate lymphoid reconstitution using a mouse (C57Bl / 6 mice) hematopoietic stem cell transplantation model. + , BMP4 + , or BMP4 + E4ORF1 +HUVECs) were generated essentially as described in the previous examples. Animals were divided into treatment and control groups as follows:

[0108] Group 1 (control) - 16 mice served as controls; these animals did not receive irradiation, bone marrow injection or endothelial cell injection.

[0109] Groups 2-4 (treatment) - Mice received total body irradiation at 1,000 cGy. All irradiated mice received an intravenous infusion of 500,000 bone marrow (BM) cells approximately 10-16 hours after irradiation. The treatment groups were as follows: Group 2 - animals received an infusion of 500,000 synthetic bone marrow cells alone. Group 3 - animals were injected with 500,000 synthetic bone marrow cells genetically engineered to express the adenovirus E4 ORF1 region and 500,000 human umbilical vein endothelial cells. An additional intravenous injection of 500,000 of these endothelial cells was given approximately 48 hours after the first injection, and again approximately 48 hours after a second injection. Group 4--animals underwent the same procedure as group 3, except that at each injection time point, the recipient endothelial cells were transduced with both E4ORF1 and BMP4.

[0110] Three weeks after irradiation, all animals were euthanized. The thymus of each animal was removed, dissected into small pieces, and subjected to collagenase digestion to release a single cell suspension consisting of the cellular components of the thymus. These cells were subjected to flow cytometric analysis to characterize the major hematopoietic and epithelial cell types present. The results were as follows:

[0111] A. Recellularization - Total Cell Recovery In irradiated animals receiving bone marrow injection alone, the number of nucleated thymocytes was reduced by more than 90% (Figure 4). Although still significantly lower compared to non-irradiated animals, co-administration of ntECs transduced with E4ORF1 alone or E4ORF1 and BMP4 significantly improved the overall cell number compared to bone marrow injection alone (Figure 4; difference from ntECs transduced with E4ORF1 alone was p<0.05, and difference from ntECs transduced with both E4ORF1 and BMP4 was p<0.01).

[0112] Total cell numbers in animals receiving ntECs transduced with the combination of E4ORF1 and BMP4 were higher than in animals receiving ntECs transduced with E4ORF1 alone - however, this difference was not statistically significant.

[0113] Among all cell lots, BM alone, E4ORF1 + ntECs, or E4ORF1 + BMP4 + Data from the ntECs-treated groups were pooled and analyzed by one-way Kruskal-Wallis ANOVA test followed up with Dunn's multiple comparison test. The Dunn test compared the mean of each group to the mean of the control bone marrow-only treated group. The data are shown in Figure 4.

[0114] B. Recovery of Hematopoietic Cells and Thymic Stromal Cells The cells of the thymus, the primary lymphoid organ important for the development of T lymphocytes, are derived from hematopoietic cells (CD45 + These may be divided into bone marrow hematopoietic stem cell-derived and thymic stromal (epithelial) cells. The recovery of these two populations, and each subpopulation, was evaluated separately and is summarized as follows:

[0115] C. CD45 + cell recovery By using donor bone marrow obtained from C57Bl / 6 mice expressing the CD45.1 isoform and host mice expressing the CD45.2 isoform, we were able to identify donor- and host-derived CD45 + It is possible to distinguish the cells.

[0116] Donor CD45 in the thymus 3 weeks after irradiation + The cell counts (Fig. 5) generally reflected the total cell counts (Fig. 4). Similar to the total cell counts, the CD45 + The cell numbers were significantly higher than those of animals that received bone marrow injection alone. Injection of ntECs transduced with both E4ORF1 and BMP4 resulted in more CD45 + The degree of cell recovery was increased, and this difference was statistically significant (Figure 5).

[0117] D. CD3 + cell recovery T-lymphocytes are characterized by the expression of the CD3 surface marker. + / CD3 + The recovery of T cell populations followed the same pattern as the recovery of CD45 (Figure 6). + Given that the majority of cells were T cells, the association with recovery of CD45 was not surprising (Figure 6B). + Similarly, the number of T cells was greatest in the group treated with ntECs transduced with both E4ORF1 and BMP4, which was statistically significantly greater than that in the group treated with ntECs transduced with E4ORF1 alone.

[0118] Importantly, CD3 also expresses CD45 +The differences in cell percentages between groups were not significant, suggesting that the differences seen in both forms of ntEC were due to enhanced CD3 recovery rather than a bias towards the CD45 subpopulation.

[0119] E. CD3 + / CD4 + and CD3 + / CD8 + Cell recovery CD3 expressing either CD4 or CD8 + The number of cells increased after ntEC-treatment (Figure 7). As with other populations, recovery after treatment with ntEC transduced with both E4ORF1 and BMP4 was greater than recovery after treatment with ntEC transduced with E4ORF1 alone.

[0120] The relative representation of these two populations (CD3 + The relative contributions of CD4 cells to CD8 cells (as a percentage of total cells) in irradiated animals were compared to those of non-irradiated animals, with a relative increase in CD8 cells and a corresponding decrease in CD4 cells (Figure 8). The average relative contributions of both populations in animals treated with ntEC transduced with both E4ORF1 and BMP4 tended to be more similar to those in non-irradiated animals than those in animals treated with no ntEC or with ntEC transduced with E4ORF1 alone.

[0121] F. Thymic Maturation Markers T lymphocyte differentiation and outgrowth within the thymus follows a well-known expression pattern of surface markers (Figure 9), which can be used to assess the effect of ntEC treatment on T lymphocyte differentiation and outgrowth, as described below.

[0122] G. Recovery of CD3 cells expressing CD4 and CD8 (double positive cells) The number of double positive (DP) cells was increased by treatment with both forms of ntEC, but like other parameters, the improvement was numerically greater with ntEC transduced with both E4ORF1 and BMP4 than with bone marrow alone (Fig. 10A). The relative number of double positive cells (percentage of total CD3+ cells) was the same in all groups, suggesting that ntEC treatment promotes but does not bias thymic T cell recovery (Fig. 10B).

[0123] H. Recovery of CD3 cells negative for CD4 and CD8 (double negative cells) The number of double negative (DN) cells was increased by treatment with both forms of ntEC, but like other parameters, was numerically improved more by ntEC transduced with both E4ORF1 and BMP4 than by bone marrow alone (Figure 11). The mean relative number of DN cells (percentage of total CD3 positive cells) was slightly lower (closer to normal) in animals treated with ntEC transduced with both E4ORF1 and BMP4 (Figure 11B).

[0124] As shown in Figure 9, double-negative CD3 + DN cells can be further subdivided into four types of cells based on their expression of CD25, the α chain of the interleukin 2 (IL-2) receptor, and CD44, an adhesion molecule involved in a wide range of cell functions such as lymphocyte activation, recirculation homing, and hematopoiesis. These four types are called DN1, DN2, DN3, and DN4, with higher numbers indicating later stages of T cell differentiation and growth.

[0125] As shown in Figure 12, the numbers of each DN subpopulation were higher in animals treated with ntECs transduced with E4ORF1 alone or with ntECs transduced with both E4ORF1 and BMP4. As with the other populations, animals treated with ntECs transduced with both E4ORF1 and BMP4 showed higher numbers than animals treated with ntECs transduced with E4ORF1 alone, but this difference was not statistically significant for any of the DN subpopulations.

[0126] As shown in Figure 13, the relative abundance of DN1, DN2, and DN4 cells / subpopulations was higher in animals treated with ntEC transduced with E4ORF1 alone or with ntEC transduced with both BMP4 and E4ORF1. The increase was accompanied by a decrease in the proportion of DN3 cells. The largest relative difference was for the earliest and least abundant subpopulations (DN1 and DN2), and the increase in animals administered ntEC transduced with both E4ORF1 and BMP4 was statistically significant.

[0127] These data show a consistent pattern in which thymic T cell repopulation is greatly improved in animals treated with ntECs, with animals receiving ntECs transduced with both BMP4 and E4ORF1 showing a greater benefit than animals receiving ntECs transduced with E4ORF1 alone. This increased recovery is evident from the earliest T cell progenitors evaluated (DN1) to the most mature single positive CD3 / CD4 and CD4 / CD8 cells.

[0128] I. Recovery of thymic stromal cells Thymic epithelial cells are characterized by the expression of EpCAM and the absence of CD45 expression. Results showed that with regard to the thymic T cell population, animals co-injected with ntECs expressed more EpCAM than animals receiving bone marrow alone. +The numbers of cells were shown (Figure 14). Interestingly, the mean number of said cells in animals receiving bone marrow alone was lower than in intact animals, whereas the mean number of cells in animals receiving ntEC was equal (E4ORF1 alone) or higher (E4+BMP4) than in intact animals (Figure 14). This is likely why a small number of animals had a very high number of EpCAM positive cells, suggesting an overshoot phenomenon (Figure 14). Data on the recovery of the various subsets of thymic stoma cells are presented below.

[0129] J. Recovery of Thymic Epithelial Progenitor Cells (TEPCs) Sca1 + (Figure 15) and Sca1 + / α6 + Analysis of thymic epithelial progenitor cells (Fig. 16) revealed that treatment with non-thymic endothelial cells increased both populations, and that this effect was enhanced by BMP4 (E4ORF1 and E4 / BMP4, respectively). Transduced It was shown that the expression of thymic endothelial cells was greatly enhanced by the expression of thymic endothelial cells.

[0130] K. Recovery of thymic cortical epithelial cells (cTECs) Analysis of mature thymic cortical epithelial cells (cTECs, Figure 17) revealed significantly more cells in E4 / BMP4-treated animals compared to bone marrow alone. + A similar trend was observed in the analysis of cTECs (Figure 18).

[0131] L. Recovery of medullary thymic epithelial cells (mTECs) Analysis of mature thymic medullary epithelial cells (mTECs, Figure 19) revealed significantly more cells in E4 / BMP4-treated animals compared to bone marrow alone. + A similar trend was observed in the analysis of mTEC (Figure 20).

[0132] The above test results are further summarized in Table 5 below, which shows that HUVEC, E4ORF1 + HUVEC, BMP4 + HUVEC, or E4ORF1+ The ability of HUVECs to promote (relatively) recovery of the listed cell type(s) in the thymus following sublethal irradiation was demonstrated (see column "Treatment Outcome"). The "+" symbol is a measure of cell number, with more "+" symbols representing higher cell numbers. For actual cell numbers, please refer to the corresponding data figures.

[0133] [Table 7]

[0134] Example 6 BMP4 + E4ORF1 + ntECs enhance survival after total body irradiation and bone marrow transplantation BMP4 + and E4ORF1 + ntECs were generated essentially as described in Example 1. C57Bl6 mice were myeloablated with 1,000cG (lethal) total body irradiation (TBI) and administered either 200,000 or 500,000 whole bone marrow cells (WBM) from synthetic mice. Some cohorts of animals (shown as "+EC" in Figure 21) were administered BMP4 after TBI. + E4ORF1 + HUVECs (500,000 cells on days 1, 3, and 5) were also administered.

[0135] Survival was assessed at the time points indicated in Figure 21. As shown in Figure 5, BMP4 + E4ORF1 + Animals treated with HUVECs (referred to as BMP4 ntECs in FIG. 5) and low doses of WBM showed reduced mortality compared to animals not treated with ntECs (64% reduced mortality, P<0.05). + E4ORF1 + Animals treated with ntECs and high doses of WBM dramatically abolished mortality (100% survival, P<0.05) (Figure 21). Thus, both doses of WBM significantly reduced BMP4 + E4ORF1+ Treatment with ntECs resulted in a dramatic and statistically significant increase in survival.

[0136] The results of the above studies were further examined in relation to the effect of BMP4 on survival after exposure to lethal doses of radiation. + E4ORF1 + The effects of ntECs are summarized in Table 6 below in a relative comparison format. The "+" symbols indicate approximate values ​​of survival rates, with more "+" symbols representing higher survival rates. For survival rate values, please refer to the corresponding data figures. + HUVECs (without E4ORF1) could not be cultured in sufficient cell numbers for use in this in vivo study (see FIG. 1).

[0137] [Table 8]

[0138] Example 7 BMP4 + E4ORF1 + Human clinical trials of ntECs BMP4 + E4ORF1 + A phase 1, open-label, multicenter, dose-escalation prospective study of ntECs was conducted in adult human subjects with hematological malignancies undergoing myeloablative conditioning (MAC) and matched related or unrelated donor (MRD or MUD) allogeneic hematopoietic cell transplantation (HCT). + E4ORF1 + The aim of this study was to evaluate the safety and preliminary efficacy of treatment with ntECs.

[0139] Use ntECs obtained from various organs (HUVECs and ECs from adipose tissue, skin, lung, heart, kidney and / or bone marrow). + E4ORF1 + The ntECs are generated as described in Example 1.

[0140] Enrolled subjects will undergo HCT planned according to review institution criteria using one of the following MAC regimens, selected by the treating oncologist: Total body irradiation (>1000 cGY) (TBI) and cyclophosphamide (120 mg / kg) (Cy / TBI). etoposide 120 mg / kg and TBI (>1000 cGY), busulfan (16 mg / kg orally or 12.8 mg / kg intravenously) and cyclophosphamide (120 mg / kg) (Bu / Cy). Busulfan (16 mg / kg orally or 12.8 mg / kg intravenously) and fludarabine (120–180 mg / m 2 )(Bu / Flu).

[0141] Other MAC regimens may be used if approved by the study medical monitor. Post-transplant immunosuppressants and supportive care will be administered according to institutional guidelines.

[0142] BMP4 + E4ORF1 + After ensuring that no acute reactions related to the stem cell infusion occurred, ntECs were administered intravenously 2 hours after completion of allogeneic (MRD or MUD) HCT infusion on day 0. If an acute infusion-related reaction occurred during the HCT infusion, the reaction was treated and the trial was discontinued.

[0143] BMP4 + E4ORF1 + The ntECs will be administered in a dose-escalating fashion, which will include four cohorts (at least six patients each), as shown in Table 7 below.

[0144] [Table 9] *********

[0145] [Section 1] A method for enhancing thymus regeneration, comprising: administering to a subject in need of thymus regeneration a therapeutic composition comprising an effective amount of genetically engineered non-thymic endothelial cells (ntECs), thereby enhancing thymus regeneration in a subject, wherein the genetically engineered ntECs are E4ORF1+ or BMP4+E4ORF1+. [Section 2] The method of paragraph 1, wherein thymic regeneration comprises recovery of at least one cell type of CD45- thymic stromal cells and CD45+ T cells. [Section 3] The method of item 1, wherein thymic regeneration comprises restoration of both CD45- thymic stromal cells and CD45+ T cells. [Section 4] The method of paragraph 2 or paragraph 3, wherein the CD45- thymic stromal cells are selected from the group consisting of thymic epithelial progenitor cells (TEPCs), cortical thymic epithelial cells (cTECs) and medullary thymic epithelial cells (mTECs). [Section 5] The method of claim 2 or 3, wherein the CD45+ T cells are selected from the group consisting of CD3+ T cells, CD4+ T cells, CD8+ T cells, double positive T cells (DP), double negative T cells (DN), double negative type 1 (DN1) T cells, double negative type 2 (DN2) T cells, double negative type 3 (DN3) T cells and double negative type 4 (DN4) T cells. [Section 6] Item 6. The method according to any one of items 1 to 5, wherein the ntEC is selected from the group consisting of umbilical vein endothelial cells (UVEC), adipose endothelial cells, skin endothelial cells, lung endothelial cells, cardiac endothelial cells, kidney endothelial cells and bone marrow endothelial cells. [Section 7] Item 7. The method according to any one of Items 1 to 6, wherein the subject is a human. [Section 8] Item 8. The method according to any one of Items 1 to 7, wherein the ntECs are human umbilical vein endothelial cells (HUVECs). [Section 9] Item 9. The method according to any one of items 1 to 8, wherein the ntECs are derived from the subject. [Section 10] Item 10. The method according to any one of items 1 to 9, wherein the ntECs are allogeneic to the subject. [Section 11] 11. The method according to any one of items 1 to 10, wherein the ntECs are MHC / HLA compatible with the subject. [Section 12] 12. The method according to any one of items 1 to 11, wherein the subject has previously been treated with chemotherapy, radiation therapy, a transplant conditioning regimen or a myeloablative conditioning regimen. [Section 1 Item 13. The method according to any one of Items 1 to 12, wherein the subject has an immunodeficiency. [Section 14] The method of claim 13, wherein the subject has an HIV infection. [Section 15] Item 15. The method according to any one of items 1 to 14, wherein the subject has an age-related deficiency in thymic tissue mass, thymic function, or T cell production. [Section 16] 16. The method according to any one of items 1 to 15, wherein the genetically engineered ntECs are administered to the subject by intravenous infusion. [Section 17] 17. The method of any of paragraphs 1 to 16, wherein the genetically engineered ntECs are administered to the subject in multiple intravenous infusions over a period of days or weeks. [Section 18] Item 18. The method according to any one of items 1 to 17, further comprising administering to the subject a therapeutic composition comprising hematopoietic stem cells (HSCs). [Section 19] The method of paragraph 18, wherein the genetically engineered ntECs and HSCs are administered simultaneously. [Section 20] The method of paragraph 18, wherein the genetically engineered ntECs and HSCs are administered to the subject in the same intravenous infusion. [Section 21] 21. The method according to any one of items 1 to 20, further comprising the step of genetically modifying the ntEC by transducing or transfecting the ntEC ex vivo with a nucleic acid molecule encoding E4ORF1 and, optionally, a nucleic acid molecule encoding BMP4, prior to administering the ntEC to the subject. [Section 22] 22. The method of any one of items 1 to 21, further comprising the step of genetically modifying autologous ntECs from the subject by transducing or transfecting the ntECs ex vivo with a nucleic acid molecule encoding E4ORF1 and, optionally, a nucleic acid molecule encoding BMP4 prior to administering the autologous ntECs to the subject. [Section 23] An isolated population of BMP4+E4ORF1+ non-thymic endothelial cells (ntECs). [Section 24] 24. The population of genetically engineered ntECs of paragraph 23, wherein the population is a substantially pure population. [Section 25] The population of genetically engineered ntECs of paragraph 23, wherein the ntECs comprise a recombinant nucleic acid molecule comprising a nucleotide sequence encoding BMP4. [Section 26] The population of ntECs described in paragraph 25, wherein a nucleotide sequence encoding BMP4 is operably linked to a heterologous promoter. [Section 27] The population of ntECs of paragraph 25, wherein the ntEC comprises a recombinant nucleic acid molecule comprising a nucleotide sequence encoding E4ORF1. [Section 28] The population of ntECs of paragraph 27, wherein the nucleotide sequence encoding E4ORF1 is operably linked to a heterologous promoter. [Section 29] 29. The population of ntECs according to any one of items 25 to 28, wherein the recombinant nucleic acid molecule is an expression vector. [Section 30] The population of ntECs of paragraph 29, wherein the expression vector is a viral vector. [Section 31] The population of ntECs of paragraph 29, wherein the expression vector is a lentiviral vector. [Section 32] The population of ntECs of paragraph 29, wherein the expression vector is a retroviral vector. [Section 33] 33. The population of ntECs according to any one of items 23 to 32, wherein the ntECs are selected from the group consisting of human umbilical vein endothelial cells (HUVECs), adipose endothelial cells, skin endothelial cells, lung endothelial cells, cardiac endothelial cells, kidney endothelial cells and bone marrow endothelial cells. [Section 34] 33. The population of ntECs according to any one of items 23 to 32, wherein the ntECs are human umbilical vein endothelial cells (HUVECs). [Section 35] A composition comprising a population of ntECs according to any one of items 23 to 34. [Section 36] A therapeutic composition comprising a population of ntECs according to any one of items 23 to 34, and a solution suitable for administration to a subject. [Section 37] A therapeutic composition comprising a population of ntECs according to any one of items 23 to 34, and a biocompatible matrix material. [Section 38] 38. The therapeutic composition of claim 37, wherein the biocompatible matrix material is liquid. [Section 39] 38. The therapeutic composition of claim 37, wherein the biocompatible matrix material is a solid. The invention is further described by the following claims.

Claims

1. A therapeutic composition for enhancing thymic regeneration in a subject, comprising genetically engineered non-thymic endothelial cells (ntECs), the genetically engineered ntECs comprising E4ORF1 ＋ or BMP4 ＋ E4ORF1 ＋ and the ntECs are human umbilical vein endothelial cells (HUVECs).

2. Thymic regeneration is associated with CD45 - Thymic stromal cells and CD45 + The therapeutic composition of claim 1, comprising restoration of at least one cell type of T cells.

3. Thymic regeneration is associated with CD45 - Thymic stromal cells and CD45 + 2. The therapeutic composition of claim 1, comprising both T cell restoration and T cell repair.

4. CD45 - The therapeutic composition of claim 2 or claim 3, wherein the thymic stromal cells are selected from the group consisting of thymic epithelial progenitor cells (TEPCs), cortical thymic epithelial cells (cTECs) and medullary thymic epithelial cells (mTECs).

5. CD45 + T cells are CD3 + T cells, CD4 + T cells, CD8 + The therapeutic composition of claim 2 or claim 3, wherein the T cells are selected from the group consisting of T cells, double positive T cells (DP), double negative T cells (DN), double negative type 1 (DN1) T cells, double negative type 2 (DN2) T cells, double negative type 3 (DN3) T cells and double negative type 4 (DN4) T cells.

6. The therapeutic composition of any one of claims 1 to 5, wherein the subject is a human.

7. The therapeutic composition of claim 1 , wherein the ntECs are autologous to the subject.

8. The therapeutic composition of claim 1 , wherein the ntECs are allogeneic to the subject.

9. The therapeutic composition of claim 1 , wherein the ntECs are MHC / HLA compatible with the subject.

10. 10. The therapeutic composition of any one of claims 1 to 9, wherein the subject has previously been treated with chemotherapy, radiation therapy, a transplant conditioning regimen or a myeloablative conditioning regimen.

11. The therapeutic composition of any one of claims 1 to 10, wherein the subject has an immunodeficiency.

12. The therapeutic composition of claim 11 , wherein the subject has an HIV infection.

13. 13. The therapeutic composition of any one of claims 1 to 12, wherein the subject has an age-related deficiency in thymic tissue mass, thymic function or T cell production.

14. 14. The therapeutic composition of any one of claims 1 to 13, administered to a subject by intravenous infusion.

15. 15. The therapeutic composition of any one of claims 1 to 14, administered to a subject by multiple intravenous infusions over a period of days or weeks.

16. A therapeutic composition according to any one of claims 1 to 15, characterized in that it is used in combination with a therapeutic composition comprising hematopoietic stem cells (HSCs).

17. 17. The therapeutic composition of claim 16, administered simultaneously with a therapeutic composition comprising hematopoietic stem cells (HSCs).

18. 17. The therapeutic composition of claim 16, administered by the same intravenous infusion as a therapeutic composition comprising hematopoietic stem cells (HSCs).

19. A method for producing genetically engineered non-thymic endothelial cells (ntECs), comprising the step of genetically modifying the ntECs by transducing or transfecting the ntECs ex vivo with a recombinant nucleic acid molecule encoding E4ORF1 and a recombinant nucleic acid molecule encoding BMP4, wherein the ntECs are human umbilical vein endothelial cells (HUVECs).

20. The method of claim 19, wherein the ntECs are derived from the subject.

21. Genetically engineered BMP4 + E4ORF1 + An isolated population of human umbilical vein endothelial cells (HUVECs), wherein the HUVECs comprise a recombinant nucleic acid molecule encoding BMP4 and a recombinant nucleic acid molecule encoding E4ORF1.

22. The population of genetically engineered ntECs of claim 21 , wherein the population is a substantially pure population.

23. The population of ntECs described in claim 21, wherein the nucleotide sequence encoding BMP4 is operably linked to a heterologous promoter.

24. The population of ntECs described in claim 21, wherein the nucleotide sequence encoding E4ORF1 is operably linked to a heterologous promoter.

25. 25. The population of ntECs of any one of claims 21 to 24, wherein the recombinant nucleic acid molecule is an expression vector.

26. The population of ntECs described in claim 25, wherein the expression vector is a viral vector.

27. The population of ntECs described in claim 25, wherein the expression vector is a lentiviral vector.

28. The population of ntECs of claim 25 , wherein the expression vector is a retroviral vector.

29. A composition comprising a population of ntECs described in any one of claims 21 to 28.

30. 30. A therapeutic composition comprising a population of ntECs according to any one of claims 21 to 28 and a solution suitable for administration to a subject.

31. 30. A therapeutic composition comprising a population of ntECs according to any one of claims 21 to 28 and a biocompatible matrix material.

32. 32. The therapeutic composition of claim 31, wherein the biocompatible matrix material is a liquid.

33. 32. The therapeutic composition of claim 31, wherein the biocompatible matrix material is a solid.

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