LPS suspension oil and LPS-containing products

Abstract

To provide: LPS fine powder, which is an LPS raw material that can be compounded in an oil main constituent cosmetic or the like without using an emulsifier; also an LPS suspension oil in which the LPS fine powder is suspended; and further an LPS compounded product in which the LPS suspension oil is compounded.SOLUTION: There are provided: LPS fine powder in which the maximum particle size of a fine powder-state LPS is 20 μm or more and 75 μm or less; an LPS suspension oil in which the LPS fine powder is suspended in oil; and a cosmetic, a cleaner, or a hairdressing agent, which is an LPS compounded product in which the LPS suspension oil is compounded.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention provides LPS This relates to products formulated using suspended oils and LPS suspended oils as raw materials, such as lip balm, oil-based cosmetics such as olive oil, balm-type or oil-type cleansing agents, and oil-based hair styling agents such as camellia oil. [Background technology]

[0002] Cosmetics and hair styling products (collectively referred to as cosmetics, etc.) are used to keep the skin clean, protect it from external stimuli, prevent dryness, and moisturize it. For this reason, cosmetics, etc., contain ingredients such as moisturizing ingredients, functional ingredients that keep the skin healthy, fragrances, and preservatives. The ingredients used come in the form of aqueous solutions, oil solutions, and powders that are dissolved in water or oil. When mixing aqueous and oil solutions, an emulsifier is used. However, aqueous ingredients cannot be blended in products that do not use emulsifiers to maintain the oil's natural transparency, or in oil-based cosmetics that are solid and do not have room for aqueous solutions.

[0003] Lipopolysaccharide (LPS) enhances the skin's natural healing ability and has recently been incorporated into cosmetics as a functional ingredient. LPS is a substance composed of lipids and sugars, but because a large proportion of water-soluble sugars is present, LPS as a whole is water-soluble but oil-insoluble. Therefore, LPS raw materials for cosmetics are provided as aqueous solutions (Somacy-CL001: Pantoea agglomerans / wheat flour fermented extract, SomacyN-CL001: Pantoea agglomerans extract, RiB-CL001: Pantoea / rice bran fermented extract, APV-CL001: Pantoea vagans culture extract) (Non-Patent Document 1). Because they are aqueous solutions, they cannot be incorporated into solid, oil-based cosmetics such as lip balms and cleansing balms, or even if they are incorporated, they are added in trace amounts that are insufficient to demonstrate their functionality. Furthermore, when LPS is added to olive oil, for example, the aqueous solution forms beads and does not blend with the oil unless an emulsifier is used.

[0004] Since the inability to incorporate water into oil-based cosmetics presents a problem, one approach is to evaporate the water from an LPS solution and formulate it as a powder. However, the recommended amount of LPS to be mixed into cosmetics is extremely small, approximately 1 μg / g, making it difficult to accurately measure and uniformly mix. To solve this problem, spray-dried LPS powders using dextrin as a carrier are also available for food-grade LPS. In this method, LPS is dissolved in a dextrin solution, and the resulting homogeneous mixture is sprayed and dried through a nozzle. As the water evaporates, the dextrin and LPS combine to form particles. The size of the spray-dried powder depends on the size of the water droplets sprayed. Spray drying using dextrin as a carrier increases the volume, making it possible to accurately measure a consistent amount of LPS. However, because the carrier used in spray drying is water-soluble, adding a spray-dried product to oil to increase the volume results in increased insoluble matter. Therefore, to incorporate LPS into oil-based cosmetics, the most suitable solution is an oil in which only LPS is suspended at an appropriate concentration in an oily solvent. However, because LPS is water-soluble and difficult to suspend in an oily solvent, no such LPS-suspended oil has existed to date. [Prior art documents] [Non-patent literature]

[0005] [Non-Patent Document 1] “LPS raw materials for sale”, [online], Natural Immunity Applied Technology Co., Ltd., [Retrieved December 28, 2020], Internet<https: / / www.macrophi.co.jp / seihin / > Summary of the Invention [Problem to be solved by the invention]

[0006] LPS fine powder is an LPS raw material that can be incorporated into oil-based cosmetics without the use of emulsifiers. is suspended The present invention provides an LPS-containing oil suspension containing LPS, and further provides an LPS-containing product containing the LPS-containing oil suspension.

[0007] The inventors discovered that even though LPS is water-soluble, by adjusting the particle size of finely powdered LPS to a specified size, it can be suspended in oil without the use of an emulsifier, making it possible to easily incorporate the required amount of LPS into oil-based cosmetics, etc.

[0008] · Raw materials that can be mixed into oil-based cosmetics without the use of emulsifiers must be oils. The oil used should be registered as a cosmetic ingredient and should be colorless, transparent, and odorless so that it can be incorporated into any cosmetic product. Water does not mix with oil, so LPS must be freeze-dried to completely evaporate the water. LPS is not soluble in oil, so it must be mixed in oil in a suspended state.

[0009] The LPS to be mixed into the oil should be finely powdered to the extent that it disperses uniformly after mixing and does not easily settle, with a maximum particle size of 20 to 75 μm, more preferably 20 to 45 μm. [Means for solving the problem]

[0010] LPS of the present invention Suspended Oil The maximum particle size of the fine powdered LPS is 20 μm or more and 75 μm or less. LPS fine powder is suspended in oil Characterized by (However, this does not include cases where emulsifiers are included.) .

[0012] The present invention also relates to an LPS-containing product containing the above-mentioned LPS suspension in oil.

[0013] Furthermore, the LPS-containing product is preferably a cosmetic, a cleanser, or a hair styling agent. [Effects of the Invention]

[0014] The present invention LPSBy using suspended oil as a raw material, it is now possible to produce oil-based cosmetics containing LPS, such as lip cream, balm cosmetics, oil cosmetics, and oil hair styling products, which were previously impossible to produce. [Brief explanation of the drawings]

[0015] [Figure 1] Photographs of sedimentation of various fine particle oil suspensions. [Figure 2] Photographs of three types of LPS-suspended oil raw materials. [Figure 3] A photo of a cleansing balm containing LPS suspended oil. DETAILED DESCRIPTION OF THE INVENTION [Example]

[0016] <Preparation of LPS fragments for suspension in oil> The gram-negative bacterium Pantoea agglomerans, which contains LPS, was cultured, heated, and centrifuged. The supernatant was treated with activated carbon, filtered through Celite, and concentrated by membrane filtration. After ethanol precipitation, the precipitate was freeze-dried to obtain freeze-dried LPS.

[0017] Approximately 6 g of the freeze-dried LPS thus obtained was crushed to pieces no larger than 1 cm square and placed in a crusher (Wonder Crusher WC-3, Osaka Chemical Co., Ltd.; processing vessel size: 108 mm diameter x 51 mm height, maximum processing capacity: 150 mL) for crushing. The rotation speed of this crusher was adjustable between 0 and 10, with a maximum rotation speed of 28,000 rpm. Generally, small crushers capable of processing tens of grams of material can become hot when operated continuously at high speed for extended periods. For crushing the freeze-dried LPS, an operating procedure that controlled heat generation was found to be appropriate: crushing at speed 7 for 50 seconds twice, followed by crushing at speed 9 for 20 seconds eight times. [Example]

[0018] <Preparation of LPS fine powder for suspension in oil> When the LPS powder preliminarily adjusted in Example 1 was suspended in oil, it was found that there were particles that precipitated in a short time. The particle size of the LPS powder pulverized under the conditions of Example 1 was in the range of 10 - 100 μm by microscopic observation. Therefore, in order to further shift the particle size distribution of the LPS crushed product to the smaller side, it was sieved. The LPS crushed product obtained in Example 1 was transferred to a stainless steel sieve (diameter 7 cm) with a medicine spoon, and while spreading the powder over the entire sieve with the medicine spoon, it was passed through the sieve. This was repeated, and the material that passed through the sieve was used as the LPS fine powder. Three types of stainless steel sieve meshes, 20 μm, 45 μm, and 75 μm, were used. The recovery rates of the LPS fine powder passing through the sieves of three mesh sizes are shown in a table.

[0019]

Table 1

[0020] From these results, it was found that when passing through a 20 - μm mesh sieve, the recovery rate was significantly low. Therefore, in the sieving operation after the crushing treatment, it is appropriate to use a sieve with a mesh size larger than 20 μm.

Example

[0021] <Adjustment of LPS suspension oil in which LPS fine powder is suspended> From the LPS powder put through a small pulverizer, three types were adjusted in the manner of Example 2: fine powder passing through a 45 - μm mesh sieve, fine powder passing through a 75 - μm mesh sieve, and fine powder that did not pass through a 75 - μm mesh sieve. While gradually adding glyceryl tri(caprylate / caprate) to 40 mg of each, stirring was carried out using a vortex mixer and a homogenizer, and finally it was made up to 100 g. The oil for suspending LPS, if it is for cosmetics, a medium - chain fatty acid (MCT) oil that is colorless, odorless, has excellent oxidation stability, low viscosity, and a refreshing feel is suitable, but it is not limited to glyceryl tri(caprylate / caprate), and natural oils such as olive oil may also be used.

[0022] After thoroughly stirring three types of adjusted LPS suspension oils, 2 mL was measured and transferred to a glass petri dish, and it was left standing at room temperature for 4 hours to observe the precipitation state. As a result, as shown in Fig. 1, precipitation was visually observed in the oil suspension with particles larger than 75 μm. No precipitation was visually observed in the oil suspension with particles of 75 μm or less. However, when observed under a magnifying glass, fine precipitation was observed. No precipitation was observed either visually or under a magnifying glass in the oil suspension with particles of 45 μm or less. From these results, it was shown that in order to obtain a stable oil suspension, a maximum particle size of 75 μm or less is good, and more preferably a maximum particle size of 45 μm or less.

Example

[0023] <Manufacture of LPS suspension oil raw material> Using LPS fine powder sieved through a 45-μm mesh, a final concentration of 100 μg / mL was produced according to the following procedure.

[0024] Since the LPS fine powder contains impurities such as proteins and nucleic acids, the LPS content in the LPS fine powder is accurately measured by the ELISA method or the Limulus method, and the amount of LPS fine powder (A) mg required to produce 1 kg of the LPS suspension oil raw material with a final LPS concentration of 100 μg / mL is calculated and used.

[0025] Into a 50-mL conical tube, (A) 595 mg of Pantoea agglomerans LPS fine powder micronized with a 45-μm mesh sieve was placed according to the method of Example 1, 4 mL of tri(caprylic acid / capric acid) glyceryl (MCT oil) was added, and it was stirred with a vortex mixer for 1 minute × 2 times. This stirring operation was repeated twice. About 500 g of MCT oil was placed in a 3-L glass beaker for tare measurement, and the LPS suspension oil in the 50-mL conical tube was added thereto. The empty 50-mL conical tube was added with 40 mL of fresh MCT oil and stirred and washed with a vortex mixer for 1 minute × 2 times, and the washing oil was added to the 3-L beaker. This washing operation was performed twice. Fresh MCT oil was added to the 3-L glass beaker so that the total weight excluding the tare became 1 kg.

[0026] The LPS fine powder and MCT oil placed in a 3 L beaker were set in a homogenizer (POLYTRON PT-MR3100, KINEMATICA AG), homogenized at a rotational speed of 8000 - 8500 rpm for 3 minutes, and then stopped for 2 minutes. This operation was repeated 8 times to obtain the LPS suspension oil raw material.

[0027]

[0028] The origin of the LPS used for the suspension oil raw material is not limited to Pantoea agglomerans (Proteobacteria phylum > γ-proteobacteria class), Enterobacter asburiae (Proteobacteria phylum > γ-proteobacteria class), and Roseomonas mucosa (Proteobacteria phylum > α-proteobacteria class). It may also be derived from gram-negative bacteria of the β-proteobacteria class of the Proteobacteria phylum, the Bacteroides phylum, or the Verrucomicrobia phylum that also have LPS.

Example

[0029] <Prototype of LPS-containing cleansing balm using LPS suspension oily raw material> Using the Pantoea agglomerans LPS suspension oil produced in Example 4, a cleansing balm was prototyped by blending the following cosmetic raw materials (Table 2). It was shown that the cleansing balm maintained appropriate solidity as a balm (Figure 3) and had good feel, detergency, and moisturizing ability when applied to the skin.

[0030]

Table 2

Claims

1. An LPS suspension oil is characterized in that fine powder of LPS having a maximum particle size of 20 μm or more and 75 μm or less is suspended in oil (excluding the case where an emulsifier is contained).

2. An LPS-containing product characterized by containing the LPS suspension oil described in claim 1.

3. The LPS-containing product according to claim 2 is a cosmetic, a cleaning agent or a hair styling agent.

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