Yeast culture medium, skin preparation for external use containing said culture medium, skin cosmetic preparation for external use containing said culture medium, and method for producing said culture medium

Abstract

To maintain or improve skin conditions, e.g. by improving the texture of skin or moisturizing the skin.SOLUTION: A culture solution, in which yeast has been grown in a medium containing white birch sap, is used to maintain or improve skin conditions.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a yeast culture medium that can be used as an external preparation for skin or a skin cosmetic, a skin preparation for skin containing the culture medium, a skin cosmetic containing the culture medium, and a method for producing the culture medium. [Background technology]

[0002] Aging care and prevention are of great interest to both men and women, and various products for this purpose, such as cosmetics and food products, are available. It is said that as the amount of sebum and water in the skin decreases with age, the moisturizing power of the stratum corneum on the surface of the skin is lost, and fine wrinkles and dry skin due to dryness are more likely to occur. In addition, due to the effects of air conditioning and heating, the skin is exposed to dryness all year round, and interest in topical skin preparations and skin cosmetics with high moisturizing and anti-aging effects is increasing.

[0003] In the past, skin cosmetics have often been tried to improve the moisturizing effect of skin and make skin lustrous by blending moisturizing agents and oil components such as polyhydric alcohols such as glycerin, hyaluronic acid, ceramide, polysaccharides, yeast extract, yeast culture liquid, vaseline, jojoba oil, etc. Also, in skin external preparations, oily components such as white vaseline, liquid paraffin, isopropyl myristate, beeswax, lanolin, stearic acid, etc. are included as bases other than the main drug. The bases in skin external preparations not only enhance the medicinal effects of the main drug, but also contribute to the protective effect and moisturizing effect of the skin.

[0004] Specifically, among skin cosmetics, many anti-aging cosmetics have been proposed from various angles to improve wrinkles, dullness, sagging, and other skin conditions that deteriorate with age (see Patent Documents 1 to 6).

[0005] By the way, Betula platyphylla (white birch) is said to be native to the temperate northern part of East Asia, and mainly grows in cool, naturally rich areas such as Hokkaido. As described below, bark extracts and sap filtrates have been proposed as raw materials for cosmetics. For example, Patent Document 7 and Patent Document 8 disclose a whitening composition that uses birch bark and is extracted with a hydrophilic solvent such as ethanol to improve arteriosclerosis and cataracts caused by aging and to have a whitening effect. Patent Document 9 discloses blended cosmetics containing gel microparticles containing birch sap components, and Patent Document 10 discloses bath additives and footbath additives containing birch sap as an ingredient.

[0006] On the other hand, there are many prior arts in which yeast culture liquid or extracts extracted from cultured yeast are used in skin external preparations and skin cosmetics, and many blended products are sold to general consumers. For example, Patent Document 11 discloses a yeast extract for cosmetics, which is cultured in a medium containing a large amount of glutamic acid, cysteine, and glycine, and extracted with a polyhydric alcohol without digestion with enzymes or proteases in the cells. Patent Document 12 discloses a composition having a heat shock protein 70 production induction effect by using a yeast cell extract that has accumulated a high concentration of zinc compounds in the cells. Patent Document 13 discloses a culture obtained by culturing yeast in a medium containing hyaluronic acids selected from the group consisting of hyaluronic acid, hyaluronic acid derivatives, and salts thereof, and a cosmetic and skin external product containing the same. Patent Document 14 discloses a fermentation liquid using nicotinamide mononucleotide and yeast, which has excellent moisturizing and texture improving effects and is also less irritating.

[0007] The culture medium for culturing yeast contains carbon sources such as glucose or other sugars as substrates, nitrogen sources such as yeast extract, peptone, corn steep liquor, and minerals such as potassium and calcium. Patent documents 15 and 16 disclose the use of a concentrate or dried product of a culture medium in which yeast fungi are cultured in a medium containing glucose, skim milk, and yeast extract as substrate components, as a pharmaceutical or cosmetic for skin. Patent document 17 discloses the use of a fermented soy milk product fermented with yeast using soy milk as a substrate component as a blastocyte proliferation agent, epidermal keratinocyte proliferation agent, collagen production promoter, hyaluronic acid production promoter, and cosmetic. [Prior art documents] [Patent documents]

[0008] [Patent Document 1] JP 2019-203033 A [Patent Document 2] JP 2007-291102 A [Patent Document 3] JP 2008-169196 A [Patent Document 4] JP 2008-255020 A [Patent Document 5] JP 2008-260721 A [Patent Document 6] JP 2009-040690 A [Patent Document 7] Japanese Patent Application Publication No. 10-15244 [Patent Document 8] JP 2001-335498 A [Patent Document 9] JP 2003-321323 A [Patent Document 10] JP 2006-131546 A [Patent Document 11] JP 2006-347988 A [Patent Document 12] JP 2014-108945 A [Patent Document 13] JP 2015-221756 A [Patent Document 14] JP 2018-145167 A [Patent Document 15] Special Publication No. 61-43033 [Patent Document 16] Special Publication No. 3-48166 [Patent Document 17] JP 2017-155029 A Summary of the Invention [Problem to be solved by the invention]

[0009] However, birch sap does not have sufficient moisturizing and texture-improving effects when used as a skin external preparation or skin cosmetic. In addition, there is no known technology for culturing yeast in a medium containing birch sap as a substrate, and it is completely unclear what effects the culture solution in which yeast is cultured has. In view of the above circumstances, the present invention aims to clarify how excellent the effects of a yeast culture solution using birch sap are, and to provide a yeast culture solution having the excellent effects, a skin external preparation containing the culture solution, a skin cosmetic containing the culture solution, and a method for producing the culture solution. [Means for solving the problem]

[0010] As a result of intensive research, the present inventors have found that when yeast is cultured in a medium containing birch sap, the resulting culture solution has an action and effect of maintaining and improving the condition of the skin, such as improving the skin texture and moisturizing the skin, and have thus completed the present invention. The present invention encompasses the following.

[0011] (1) A culture medium in which yeast is grown in a medium containing birch sap. (2) The culture medium according to (1), wherein the birch sap is sap obtained from Betula platyphylla. (3) A culture medium according to (1) or (2), characterized in that the yeast is one or more yeasts selected from the group consisting of yeasts belonging to the genus Galactomyces, yeasts belonging to the genus Saccharomycopsis, and yeasts belonging to the genus Saccharomyces. (4) A skin topical preparation comprising the culture medium described in any one of (1) to (3) above. (5) A skin cosmetic preparation comprising the culture medium according to any one of (1) to (3) above. (6) A skin cosmetic composition according to (5), which exhibits at least one effect selected from the group consisting of a moisturizing effect, a skin texture improving effect, and a dullness improving effect. (7) A method for producing a culture medium, characterized in that during the process of culturing yeast in a medium containing birch sap, low molecular weight sugars in the birch sap are consumed as a nutrient source for the yeast, resulting in a lower concentration of low molecular weight sugars in the birch sap compared to before the start of cultivation. Effect of the Invention

[0012] The culture solution according to the present invention can maintain and improve skin conditions, such as improving skin texture and moisturizing effects. In particular, the culture solution according to the present invention has an extremely excellent effect of maintaining and improving skin conditions, compared to birch sap in which yeast fungi are not cultured.

[0013] The skin external preparation and skin cosmetic according to the present invention contain the culture solution, and therefore can maintain and improve skin conditions such as skin texture improving effect and moisturizing effect. Furthermore, the method for producing a culture solution according to the present invention can produce a culture solution with a low concentration of low molecular weight sugars that can maintain and improve skin conditions such as skin texture improving effect and moisturizing effect. [Brief description of the drawings]

[0014] [Figure 1] FIG. 2 is a characteristic diagram showing the results of measuring the glucose concentration for Production Examples 1 to 4 prepared in Example 1. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0015] The present invention will be described in detail below.

[0016] The culture solution to which the present invention is applied is a culture solution in which yeast fungus is cultured in a medium containing birch sap. Here, birch sap means sap collected from birch. Examples of birch include Japanese white birch (scientific name: Betula platyphylla, synonym: Betula pendula var. platyphylla), American white birch (scientific name: Betula papyrifera), European white birch (scientific name: Betula pendula, also known as Betula pubescens), and Betula szechuanica (scientific name: Betula pendula var. szechuanica). Among them, the sap of Japanese white birch is preferably sap collected from Japanese white birch classified as Japanese white birch in the broad sense (scientific name: Betula platyphylla). Examples of Japanese white birch classified as Japanese white birch in the broad sense (scientific name: Betula platyphylla) include Betula platyphylla var. platyphylla, Betula platyphylla var. japonica, Betula platyphylla var. japonica f. laciniata, Betula platyphylla var. kamtschatica, and Betula platyphylla var. mandshurica.

[0017] The birch sap may be sap collected from one of the above-mentioned birch species, or may be a mixture of sap collected from multiple birch species. Birch sap is collected, for example, by drilling holes or making linear cuts in the trunks of birch trees. The components of the collected sap may vary depending on the habitat and season of the tree. For example, the collected sap is about 99% water, and contains monosaccharides such as glucose and fructose as the remaining solid content, and potassium, sodium, calcium, magnesium, manganese, iron, zinc, phosphorus, barium, silicon, boron, lanthanum, strontium, thorium, aluminum, chromium, etc. as minerals. In addition, birch sap may contain other amino acids such as glutamic acid, aspartic acid, valine, isoleucine, methionine, leucine, lysine, phenylalanine, glycine, alanine, threonine, arginine, cystine, cysteine, proline, serine, tyrosine, and histidine, as well as organic acids such as malic acid and succinic acid, proteins, and polysaccharides.

[0018] The yeast fungus to be cultured in the medium containing birch sap is not particularly limited, and yeast fungus disclosed in The Yeasts, Fifth Edition: A Taxonomic Study, etc. can be used. Examples of yeast fungus include yeasts of the genera Candida, Galactomyces, Saccharomycopsis, Saccharomyces, and Pichia. In particular, one type of yeast fungus may be cultured in the medium containing birch sap, but multiple yeast fungus may also be used for culture simultaneously or at different times.

[0019] In particular, it is preferable to use, as the Galactomyces yeast among the yeasts, for example, Galactomyces reessii, Galactomyces geotrichum, Galactomyces candidus, Galactomyces pseudocandidus, etc. Furthermore, it is preferable to use, as the Saccharomycopsis yeast among the yeasts, for example, Saccharomycopsis capsularis, Saccharomycopsis crataegensis, Saccharomycopsis malanga, etc. Furthermore, as the yeast fungus of the genus Saccharomyces, it is preferable to use, for example, Saccharomyces cerevisiae, Saccharomyces bacillaris, etc. Furthermore, as the yeast fungus of the genus Saccharomyces, commonly used beer yeast, sake yeast, wine yeast, baker's yeast, etc. may be used. As for these yeast fungus examples, one kind may be used alone, or two or more different kinds of yeast fungus may be used in combination.

[0020] The birch sap contained in the yeast culture medium may be in any form, for example, it may be a squeezed raw liquid or a crude filtrate of the raw liquid. The birch sap used is preferably one from which admixtures, impurities, and insoluble solids have been removed by filtration. Furthermore, the birch sap may be one obtained by diluting the raw liquid with a solvent such as water to a specified ratio, or one obtained by concentrating the same to a specified ratio. However, it is most preferable to use the birch sap at the component concentration as it is, without diluting or concentrating it.

[0021] In addition, it is preferable that the birch sap is sterilized before culturing the yeast. That is, when the birch sap is added to a normal yeast culture medium, the birch sap may be sterilized before being added to the medium, or the medium itself to which the birch sap has been added may be sterilized. Sterilization of the birch sap or the medium containing the birch sap can be performed by any method, such as heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, or ozone sterilization. For example, the birch sap or the medium containing the birch sap may be heat sterilized at 65° C. or higher, for example, 110 to 130° C. or 120 to 130° C., and in one example, it can be sterilized by treating at 121° C. for 20 minutes. After heat sterilization, it is preferable to cool the medium to a temperature suitable for culturing the yeast before using it for yeast culture.

[0022] Cultivation of yeast in a medium containing birch sap can be carried out by inoculating the medium with yeast and culturing it. Here, the medium may be a single composition of birch sap alone, or a mixed composition containing a normal yeast medium and birch sap. A normal yeast medium is a medium containing components necessary for culturing yeast (carbon source, nitrogen source, etc.). A normal yeast medium contains components selected from yeast extract, peptone, casein, soybean hydrolyzate, malt extract, amino acids, etc. as nitrogen sources necessary for culturing. In addition, a normal yeast medium contains glucose, etc. as a carbon source, and mineral components such as sodium salt, potassium salt, calcium salt, and magnesium salt. In addition, when birch sap contains a sufficient amount of carbon source and / or mineral components for culturing yeast, the yeast medium used in combination with birch sap may have a composition in which the carbon source and / or mineral components are removed from the normal composition. In particular, a medium suitable for culturing yeast contains birch sap, yeast extract, and peptone.

[0023] In culturing yeast in a medium containing birch sap, the amount of sugar as a carbon source contained in the medium is preferably 0.05 to 5 g / 100 ml in terms of glucose, more preferably 0.1 to 2 g / 100 ml in terms of glucose. Here, birch sap contains about 1% by weight of sugar components such as glucose and xylose. Therefore, birch sap can be used as it is without diluting or concentrating. By using birch sap as it is, it can fully exert its effects of maintaining and improving the skin condition, such as improving the texture and moisturizing the skin.

[0024] The birch sap may be used after diluting or concentrating it to a predetermined ratio. The concentrations of the carbon source and mineral components contained in the birch sap can be adjusted to a desired range by diluting or concentrating it. It is also possible to add sugar such as glucose to the birch sap so that the carbon source is in the desired range. Furthermore, mineral components can also be added to the birch sap to achieve the desired concentration.

[0025] When culturing yeast in a medium containing birch sap, the yeast may be directly added (inoculated) to the medium, but it is preferable to add a pre-culture medium in which the yeast has been pre-cultured. By adding a pre-culture medium, the culture time for the main culture can be shortened and the risk of contamination can be reduced. The medium used for the pre-culture may have the same composition as the medium used for the main culture, or a normal yeast medium that does not contain birch sap may be used. In particular, it is most preferable to use a medium containing birch sap in this pre-culture to provide the carbon source and mineral components necessary for yeast culture.

[0026] By carrying out the pre-culture, the yeast can be transferred to the main culture while maintaining the yeast in the logarithmic growth phase, and therefore the production efficiency in the mass culture of the yeast can be significantly improved. The conditions for culturing the yeast are not particularly limited as long as they allow the yeast to grow. The culture temperature is not particularly limited as long as it allows the yeast to grow, but it can be typically 15 to 45°C, preferably 25 to 40°C, for example 28 to 37°C. The pH during the culture is not particularly limited as long as it allows the yeast to grow, but it can be 3.0 to 8.0, preferably 4.0 to 7.0, for example 5.0 to 7.0.

[0027] Yeast fungi can convert sugars to carbon dioxide and use it as an energy source under aerobic conditions, and it is known that yeast fungi grow and grow well under these conditions. On the other hand, under oxygen-poor conditions, the growth slows down and produces alcohol and carbon dioxide or organic acids by homo- or heterofermentation. In particular, when culturing yeast fungi in a medium containing birch sap, it is preferable to culture the yeast fungi under aerobic conditions. By culturing the yeast fungi under aerobic conditions, a culture solution having effects of maintaining and improving the skin condition, such as improving the skin texture and moisturizing effects, can be produced. To aerobically culture yeast fungi in a medium containing birch sap, an aeration and stirring type fermenter used for ordinary microbial culture can be used. In this case, the amount of aeration is not particularly limited, but can be, for example, 0.01 to 5 vvm, preferably 0.1 to 2 vvm, and more preferably 0.3 to 1.5 vvm. The stirring speed of the medium is not particularly limited, and conditions suitable for the growth of yeast fungi can be arbitrarily set.

[0028] In addition, during the cultivation of yeast, substances that change the pH, such as organic acids, may be produced, and the change in pH may inhibit the growth of yeast. For example, when organic acids are accumulated during the cultivation of yeast, the pH of the medium decreases, so it is preferable to continue the cultivation by adding a pH adjuster to adjust the pH to the above range, preferably pH 4.0 to 7.0, for example, 5.0 to 7.0.

[0029] Furthermore, the time for culturing the yeast in the medium containing birch sap is not particularly limited, but can be, for example, 5 hours to 1 month, such as 1 to 10 days or 1 to 5 days, and preferably 1 to 3 days.

[0030] In the manner described above, yeast fungus can be cultured in a medium containing birch sap to produce a culture solution. The culture solution obtained has excellent characteristics such as maintaining and improving skin conditions, such as skin texture improving effects and moisturizing effects. In particular, the culture solution obtained is extremely superior in terms of the degree to which it maintains and improves skin conditions, such as skin texture improving effects and moisturizing effects, compared to the use of birch sap alone. Therefore, the culture solution obtained can be used as a skin external preparation or skin cosmetic.

[0031] In addition, the culture fluid obtained by culturing yeast in a medium containing birch sap has a reduced sticky feeling compared to birch sap. Therefore, skin topicals and skin cosmetics containing this culture fluid can maintain and improve skin conditions such as improving skin texture and moisturizing effects, and also have a refreshing feel when used.

[0032] Furthermore, the obtained culture solution may be used as an external preparation for skin or a skin cosmetic as it is, or may be used as an external preparation for skin or a skin cosmetic after a process such as filtration. The obtained culture solution is not limited to a filtration process, and it is preferable to carry out a process of exuding bacterial components or components accumulated in the bacterial cells before the filtration process. This process is not particularly limited, but may be, for example, lysed by the action of digestive enzymes such as protease, lipase, amylase, etc., or may be decomposed with acid after separating the bacterial cells, or the yeast may be physically crushed by means of high-speed rotary crushing, ultrasonic crushing, freeze-drying crushing, etc., or may be heated to sterilize. These processes allow the useful components contained in the yeast to be rationally extracted into the culture solution.

[0033] As described above, the culture solution obtained by culturing yeast in a medium containing birch sap can be used as an external skin preparation or skin cosmetic as it is, but the culture solution that has undergone the above-mentioned various steps can also be used as an external skin preparation or skin cosmetic. Therefore, in this specification, the term "culture solution" includes not only the culture solution obtained by culturing yeast in a medium containing birch sap, but also a solution obtained after filtering the culture solution, a solution obtained after passing through the above-mentioned various steps, a solution obtained by filtering the solution, and a solution obtained by performing one or more treatments such as centrifugation, deodorization and decolorization using a filter aid such as activated carbon or diatomaceous earth, or a combination of these. In this specification, the "culture solution" also includes a solution obtained by concentrating, diluting, drying, or microfiltration of these solutions, or a solution purified by centrifugation or the like. The purification method is not particularly limited, but examples thereof include filtration using a filter such as centrifugal filtration or a membrane filter.

[0034] In particular, as described above, it is preferable to heat the culture solution obtained by culturing yeast in a medium containing birch sap at high temperatures to exude useful components from the yeast cells into the culture solution. More specifically, the culture solution obtained is heated at 121°C for 20 minutes or more, and the resulting extract is centrifuged or filtered (e.g., microfiltration) or both to obtain a filtrate from which impurities, cells, insoluble matter, etc. have been removed. The exudation of useful components may be achieved by cell disruption using a high-pressure homogenizer, ultrasonic homogenizer, high-speed rotary homogenizer, etc., in addition to thermal cell decomposition, or by a combination of a plurality of these methods. In addition, solid contents such as cell walls of cells may be removed together with impurities and insoluble matter, etc., by filtration, for example, through a filter.

[0035] The obtained culture solution can be filtered by any method. For example, the filtration can be performed using a filter or other filtration device suitable for removing impurities, bacterial cells, and / or insoluble matter. Examples of filtration using a filter or other filtration device include centrifugal filtration, pressure filtration, and suction filtration. The filter used to filter the culture solution is not particularly limited, and examples include membrane filters, glass fiber filters, cellulose filters, and sintered filters. Filtration can be performed using one of these filters, or two or more types of filters in combination. More specifically, when a precision filtration filter is used, a filter having a pore size of typically less than 1 μm, preferably 0.5 μm or less, more preferably 0.1 μm to 0.5 μm or 0.2 μm to 0.5 μm, for example 0.45 μm, 0.22 μm, or 0.1 μm can be used. In addition, filtration using a precision filtration filter may be performed after filtration using diatomaceous earth filtration or a prefilter.

[0036] The obtained culture solution can be diluted or concentrated by any dilution or concentration method. When diluting, for example, water or any other solvent can be used. The culture solution can be concentrated by, for example, heat evaporation concentration method, freeze concentration method, ultrafiltration method, dialysis method, chromatography method, etc.

[0037] Any method for sterilizing or disinfecting the obtained culture solution can be used, including, for example, heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, ozone sterilization, etc.

[0038] When the obtained culture solution is dried to obtain a dried product, any drying method can be applied. Examples of the method for drying the culture solution include freeze-drying methods such as vacuum freeze-drying, and thermal drying methods such as spray drying. The obtained culture solution can be deodorized and / or decolorized by any deodorizing and / or decolorizing method, such as a method in which the culture solution is passed through activated carbon to adsorb odorous and / or attractive components onto the activated carbon.

[0039] The culture solution may be subjected to a wintering (dewaxing) treatment, in which the culture solution is cooled to precipitate components that solidify at low temperatures (wax), and the wax can be removed by solid-liquid separation using a method such as filtration.

[0040] Here, topical skin preparations refer to pharmaceuticals containing ingredients (main drugs) effective for the diagnosis, treatment, or prevention of diseases in humans or animals, or bases used in such pharmaceuticals. Topical skin preparations include, for example, pharmaceuticals and quasi-drugs as defined in the "Act on Ensuring Quality, Effectiveness, and Safety of Pharmaceuticals, Medical Devices, etc." in Japan. On the other hand, skin cosmetics refer to cosmetics that are not classified as pharmaceuticals or quasi-drugs and are used on human skin. Topical skin preparations refer to cosmetics that are used on human skin as defined in the "Act on Ensuring Quality, Effectiveness, and Safety of Pharmaceuticals, Medical Devices, etc." in Japan.

[0041] The topical skin preparation may be in any dosage form, and examples thereof include ointments, patches, poultices, liniments, lotions, creams, extracts, liquid extracts, tinctures, elixirs, syrups, limonades, aromatic perfumes, liquids, suspensions, emulsions, medicated bath additives, medicated shampoos, aerosols, powders, granules, tablets, capsules, and the like.

[0042] In addition, the skin external preparation and skin cosmetic may be a composition in any form, such as a solution, suspension, emulsion, powder, paste, mousse, or gel. In particular, the skin cosmetic may be in any form that can be used by contacting the skin, specifically, for example, lotion, milky lotion, beauty essence, general cream (face cream, hand cream, body cream, etc.), face wash (cleansing cream, etc.), pack, shaving cream, suntan cream, sunscreen cream, sunscreen lotion, suntan lotion, cosmetic soap, foundation, face powder, powder, lipstick, lip balm, eyeliner, eye cream, eye shadow, mascara, bath additive (bath additive), shampoo, hair rinse, hair treatment, hair pack, scalp care agent, body rinse, body gel, hair dye, hair cosmetics, etc. Particularly preferred are lotion, milky lotion, beauty essence, face cream, face wash, etc.

[0043] Furthermore, in skin topical preparations and skin cosmetics, the above-mentioned culture solution can be formulated in any form. For example, it may be simply mixed with other ingredients as is, or it may be formulated in the form of a gel, powder, granules, or an encapsulated body.

[0044] In addition to the above-mentioned culture solution, the skin cosmetic preparation may contain additives acceptable for cosmetics, and other active ingredients, etc. as necessary. Such additives and other active ingredients can be selected as appropriate for the intended use and form. The skin cosmetic preparation can be produced according to any cosmetic production method, for example, by mixing, stirring, molding, filling, etc., of the raw materials.

[0045] The skin external preparation can be a composition containing the above-mentioned culture solution as a main agent that maintains and improves the skin condition, such as skin texture improving effect and moisturizing effect. Alternatively, the skin external preparation can be a composition containing a predetermined medicinal ingredient (main agent) and the above-mentioned culture solution as a base that maintains and improves the skin condition, such as skin texture improving effect and moisturizing effect. The skin external preparation can contain the above-mentioned culture solution, pharma- ceutical acceptable additives, and other active ingredients as necessary. Such additives and other active ingredients can be arbitrarily selected and used according to the purpose and form of the medicine. The medicine according to the present invention can be manufactured by mixing, stirring, molding, filling, etc. of raw materials according to any medicine manufacturing method.

[0046] The topical skin preparations and skin cosmetics may contain one or more additives for formulations, such as carriers (solid or liquid carriers), excipients, surfactants, binders, disintegrants, lubricants, odorants, solubilizing agents, suspending agents, coating agents, pH adjusters, colorants, fragrances, flavorings, cooling agents, stabilizers, foaming agents, preservatives, and buffers.

[0047] The skin external preparation and skin cosmetic preparation may contain, for example, one or more components selected from any of the following groups. Any component selected from proteins and protein hydrolysates such as collagen, collagen hydrolysate, gelatin, gelatin hydrolysate, elastin, elastin hydrolysate, lactoferrin, keratin, keratin hydrolysate, casein, albumin, royal jelly-derived protein hydrolysate, and honey-derived protein hydrolysate may be included. Any component selected from natural polymers and derivatives thereof such as sodium hyaluronate and hyaluronic acid derivatives, xanthan gum, carrageenan, guar gum, alginic acid and its salts, pectin, chondroitin sulfate and its salts, water-soluble chitin, chitosan derivatives and its salts, pullulan, deoxyribonucleic acid, gum arabic, and gum tragacanth may be included. Any component selected from herbal medicines such as ground cherries, monk fruit, jujube, pearl barley, licorice, eucommia, ginger, and udo may be included. It may contain any component selected from seaweed powder, seaweed extract, and polysaccharides derived from seaweed. It may contain any component selected from animal-derived substances such as placenta extract. It may contain any component selected from water such as carbonated water, hot spring water, micro-nano bubble water, purified water, and distilled water. It may contain any component selected from acidic polymers such as carboxyvinyl polymers and their salts, polyacrylic acid and its salts, carboxymethylcellulose and its salts, and neutral polymers such as polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinyl pyrrolidone, and nitrocellulose polyvinyl methyl ether. It may contain any component selected from cationic polymers such as cationized cellulose, polyethyleneimine, and cationized guar gum. It may contain any component selected from lower alcohols such as ethanol and isopropyl alcohol. It may contain any component selected from ultraviolet absorbers such as para-aminobenzoic acid-based ultraviolet absorbers, salicylic acid-based ultraviolet absorbers, cinnamic acid-based ultraviolet absorbers, anthranilate-based ultraviolet absorbers, and benzophenone-based ultraviolet absorbers.It may contain any component selected from anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, and allantoin. It may contain any component selected from antioxidants such as stearic acid esters, nordihydroguaiaretenic acid, dibutyl hydroxytoluene, butyl hydroxyanisole, parahydroxyanisole, propyl gallate, sesamol, sesamolin, and gossypol. It may contain any component selected from parabenzoic acid esters such as methyl parabenzoate, ethyl parabenzoate, propyl parabenzoate, and butyl parabenzoate, and preservatives such as sorbic acid, dehydroacetic acid, phenoxyethanol, and benzoic acid. It may contain any component selected from edetic acid and its salts such as edetic acid and disodium edetate, phytic acid, and hydroxyethane disulfonic acid, and sequestering agents. It may contain any component selected from polyhydric alcohols such as glycerin, 1,3-butylene glycol, and propylene glycol. It may contain any component selected from amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, glycine, and salts thereof. It may contain any component selected from sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, honey, and the like. It may contain any component selected from bases such as ammonia water, monoethanolamine, triethanolamine, sodium hydroxide, and potassium hydroxide, and the like. It may contain any component selected from hydrocarbons such as liquid paraffin, squalane, and vaseline, and the like. It may contain any component selected from fats and oils such as coconut oil, evening primrose oil, jojoba oil, castor oil, and hardened castor oil, and the like. It may contain any component selected from fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and behenic acid, and the like. It may contain any component selected from higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol, and the like. It may contain any component selected from esters such as isopropyl myristate, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate, and stearyl stearate.It may contain any component selected from phospholipids such as lecithin and its derivatives. It may contain any component selected from animal and plant-derived lipids such as bovine bone marrow fat and bovine brain lipid. It may contain any component selected from alkyl sulfates such as ammonium lauryl sulfate, ethanolamine lauryl sulfate, sodium lauryl sulfate, and triethanolamine lauryl sulfate. It may contain any component selected from polyoxyethylene alkyl sulfates such as polyoxyethylene (2EO) lauryl ether sulfate triethanolamine (EO is ethylene oxide, and the number before EO indicates the number of moles of ethylene oxide added; the same applies below) and polyoxyethylene (3EO) alkyl (one of 11 to 15 carbon atoms or a mixture of two or more) ether sulfate. It may contain any component selected from alkyl benzene sulfonates such as sodium lauryl benzene sulfonate and triethanolamine lauryl benzene sulfonate. It may contain any component selected from polyoxyethylene alkyl ether sulfates such as polyoxyethylene (3EO) tridecyl ether acetate sodium.N-Acylamino acid salts such as sodium coconut oil fatty acid sarcosine, triethanolamine lauroyl sarcosine, sodium lauroyl methyl-L-glutamate, sodium coconut oil fatty acid-L-glutamate, triethanolamine coconut oil fatty acid-L-glutamate, sodium coconut oil fatty acid methyl taurate, sodium lauroyl methyl taurate, sodium ether sulfate alkane sulfonate, sodium hydrogenated coconut oil fatty acid glycerin sulfate, sodium hydrogenated coconut oil fatty acid glycerin sulfate, disodium undecynoyl amidoethyl sulfosuccinate, sodium octylphenoxydiethyl sulfonate, oleic acid The surfactant may include any component selected from anionic surfactants such as disodium aminosulfosuccinate, dioctyl sodium sulfosuccinate, dioctyl sodium sulfosuccinate, disodium lauryl sulfosuccinate, polyoxyethylene alkyl (carbon chain 12-15) ether phosphate (8-10EO), sodium polyoxyethylene oleyl ether phosphate, sodium polyoxyethylene cetyl ether phosphate, disodium lauryl polyoxyethylene sulfosuccinate, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, and sodium tetradecene sulfonate. The surfactant may include any component selected from cationic surfactants such as stearyl dimethyl ammonium chloride, di(polyoxyethylene)oleyl methyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, stearyl trimethyl ammonium chloride, tri(polyoxyethylene)stearyl ammonium chloride, polyoxypropylene methyl diethyl ammonium chloride, myristyl dimethyl benzyl ammonium chloride, and lauryl trimethyl ammonium chloride.The surfactant may include any component selected from amphoteric surfactants such as 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, sodium undecyl hydroxyethyl imidazolinium betaine, sodium undecyl-N-hydroxyethyl-N-carboxymethyl imidazolinium betaine, stearyl dihydroxyethyl betaine, coconut oil fatty acid amidopropyl betaine, sodium coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethyl imidazolinium disodium lauryl sulfate, and N-coconut oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate. It may contain any component selected from nonionic surfactants such as polyoxyethylene alkyl (carbon number 12 to 14) ether (7EO), polyoxyethylene octylphenyl ether, polyoxyethylene oleyl ether, polyoxyethylene glycerin oleate, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxyethylene cetylstearyl diether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyl tetradecyl ether, polyoxyethylene lanolin, polyoxyethylene lanolin alcohol, and polyoxypropylene stearyl ether. It may contain any component selected from thickeners such as isostearic acid diethanolamide, undecylenic acid monoethanolamide, oleic acid diethanolamide, beef tallow fatty acid monoethanolamide, hardened beef tallow fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristic acid diethanolamide, coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, and lanolin fatty acid diethanolamide.The composition may contain any component selected from linear or cyclic methyl polysiloxane, methylphenyl polysiloxane, dimethyl polysiloxane polyethylene glycol copolymer, dimethyl polysiloxane polypropylene copolymer, amino-modified silicone oil, quaternary ammonium-modified silicone oil, and other silicone oils. The composition may contain any component selected from thioglycolic acid and its salts. The composition may contain any component selected from cysteamine and its salts. The composition may contain any component selected from peroxides such as hydrogen peroxide, persulfates, perborates, and urea peroxide. The composition may contain any component selected from bromates such as sodium bromate and potassium bromate. The composition may contain any component selected from other active ingredients such as keratolytic agents, astringents, wound healing agents, deodorants, antiallergic agents, blood flow promoters, and cell activators.

[0048] By the way, since the skin external preparation and skin cosmetic contain the above-mentioned culture solution, they can maintain and improve the skin condition, such as the skin texture improving effect and moisturizing effect. Here, maintaining and improving the skin condition means maintaining and / or improving the moisturizing property of the skin, maintaining and / or improving the skin texture, and preventing and / or improving the dullness of the skin. Specifically, the skin texture improving effect is the effect of improving the skin to a fine texture, that is, the skin grooves on the skin surface are shallow, and each of the skin ridges shows a clear, small and beautiful triangle. In addition, the dullness prevention effect is the effect of acting on the skin part that is lower in brightness compared to the surrounding area, called dull skin, narrowing the area of ​​dull skin and reducing the degree of decrease in brightness.

[0049] The skin topical agent and skin cosmetic preparation are particularly useful for administration or application to a subject having at least one of the symptoms of dry skin, dull skin, and rough skin. Such a subject can be, for example, a subject having factors that tend to cause at least one of dry skin, dull skin, and rough skin. Factors that tend to cause at least one of dry skin, dull skin, and rough skin can be, for example, a subject having environment, lifestyle, dietary habits, genetic factors, constitution, disease, mental and physical stress, aging, etc. By applying the skin topical agent and skin cosmetic preparation to a subject having these factors, at least one of dry skin, dull skin, and rough skin can be improved, and health or beauty benefits can be expected.

[0050] The dosage and application amount of the skin topical agent and skin cosmetic varies depending on the age, body weight, application / administration route, dosage form, number of administrations, etc. of the subject, and can be changed widely at the discretion of a person skilled in the art. Specifically, the application amount or administration amount is not particularly limited, but can be, for example, 0.001 mg / time to 10 g / time, calculated as the dry weight of the culture solution, which is the active ingredient. The skin topical agent and skin cosmetic may be repeatedly administered or applied at intervals of, for example, 6 to 24 hours, but is not limited thereto. EXAMPLES

[0051] The present invention will be described in more detail below with reference to examples, but the technical scope of the present invention is not limited to the following examples.

[0052] [Example 1] Preparation of sap yeast culture and control All of the birch sap used in this example was produced in Hokkaido and was filtered from the original solution without any processing such as concentration or dilution.

[0053] <Production Example 1> Galactomyces reessii A pre-culture medium was prepared by using birch sap from Hokkaido as the substrate for pre-culture, and adding yeast extract and peptone as supplementary nutrients (see Table 1). The pre-culture medium was placed in a baffled flask, covered with silicone, and sterilized at 121°C for 20 minutes. After sterilization, the pre-culture medium was cooled to 30°C, and one loopful of Galactomyces reessii was inoculated. In this Production Example 1, the pre-culture medium was used for shaking pre-culture at 30°C for 48 hours.

[0054] For the main culture, birch sap (2000 g) roughly filtered through a cartridge filter with a mesh size of 20 μm was placed in a small jar fermenter for microbial culture and sterilized at 121°C for 20 minutes. After cooling the birch sap to 30°C, 50 g of preculture medium was added, and Galactomyces raceii was cultured at 30°C while continuously adjusting the pH to 6.0 with an aqueous solution of sodium hydroxide. During the culture period, samples were taken at appropriate times, and the glucose concentration in the culture solution was measured using an enzymatic method (F Kit Glucose: JK International Co., Ltd.). The glucose content in the culture solution is shown in Figure 1. As shown in Figure 1, glucose was consumed and decreased as the bacteria grew, and the glucose content, which was initially 1200 ppm, fell to less than 20 ppm at the end of the culture.

[0055] The resulting culture solution was heated at 121°C for 30 minutes to extract the bacterial components, and then cooled to room temperature. The resulting solution containing solids such as yeast cell walls was filtered through a prefilter and then a 0.45μm membrane filter to remove the solids, yielding a birch sap Galactomyces culture solution.

[0056] <Production Example 2> Saccharomycopsis capsularis In this Production Example 2, a birch sap Saccharomycopsis culture solution was obtained in the same manner as in Production Example 1, except that Saccharomycopsis capsularis was used. The change in glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1).

[0057] <Production Example 3> Saccharomyces cerevisiae In this Production Example 3, except that Saccharomyces cerevisiae was used, a birch sap Saccharomyces culture solution was obtained in the same manner as in Production Example 1. The change in glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1).

[0058] <Production Example 4> Cultivation with multiple bacteria In this Production Example 4, a birch sap Galactomyces-Saccharomyces culture solution was obtained in the same manner as in Production Example 1, except that Galactomyces reessii and Saccharomyces cerevisiae were used. The change in glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1). In addition, in Production Example 4, the obtained birch sap Galactomyces-Saccharomyces culture solution was vacuum freeze-dried to obtain a birch sap Galactomyces-Saccharomyces culture solution freeze-dried powder.

[0059] <Comparative Example 1> Uncultured birch sap In Comparative Example 1, birch sap (2000 g) roughly filtered using a cartridge filter with a mesh size of 20 μm was sterilized at 121° C. for 20 minutes. The sterilized birch sap was cooled to room temperature and filtered through a prefilter and then a 0.45 μm membrane filter to prepare a sterilized birch sap filtrate. The glucose concentration of this liquid was measured using an enzymatic method (F Kit Glucose: JK International Co., Ltd.).

[0060] <Comparative Example 2> Yeast culture in skim milk medium In Comparative Example 2, skim milk medium was prepared as a substrate for pre-culture (see Table 1). The prepared pre-culture medium was placed in a baffled flask, covered with silicone, and sterilized at 121°C for 20 minutes. After sterilization, the pre-culture medium was cooled to 30°C, and one loopful of Galactomyces reessii was inoculated. In this Comparative Example 2, this pre-culture medium was used for shaking culture at 30°C for 48 hours.

[0061] For the main culture, 20g of skim milk, 10g each of glucose and yeast extract were dissolved in 1960g of purified water to prepare the main culture medium, which was placed in a small jar fermenter for microbial culture and sterilized at 121°C for 20 minutes. After the main culture medium was cooled to 30°C, 50g of preculture medium was added, and Galactomyces raceii was cultured at 30°C for 48 hours while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. The obtained skim milk Galactomyces culture solution was heated at 121°C for 30 minutes to extract the bacterial components, and then cooled to room temperature. The obtained solution containing solids such as yeast cell walls was filtered through a prefilter and then a 0.45μm membrane filter to obtain a skim milk Galactomyces culture solution from which solids had been removed.

[0062] <Comparative Example 3> Yeast culture in soy milk medium In Comparative Example 3, soy milk medium was prepared as a substrate for pre-culture (see Table 1). The prepared pre-culture medium was placed in a baffled flask, covered with silicone, and sterilized at 121°C for 20 minutes. After sterilization, the pre-culture medium was cooled to 30°C, and one loopful of Galactomyces reessii was inoculated. In this Comparative Example 3, this pre-culture medium was used for shaking culture at 30°C for 48 hours.

[0063] For the main culture, 2000g of soy milk was placed in a small jar fermenter for microbial culture and sterilized at 121℃ for 20 minutes. After the main culture medium was cooled to 30℃, 50g of preculture was added, and Galactomyces raceii was cultured at 30℃ for 48 hours while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. The obtained soy milk Galactomyces culture solution was heat-treated at 121℃ for 30 minutes to extract the bacterial components, and then cooled to room temperature. The obtained solution containing solids such as yeast cell walls was filtered through a prefilter and then a 0.45μm membrane filter to obtain a soy milk Galactomyces culture solution from which the solids had been removed.

[0064] [Table 1]

[0065] Example 2 The following describes the evaluation test methods and results using the various culture media produced in Example 1 and the culture media of the comparative examples. Table 2 shows a list of the samples used for the evaluation.

[0066] [Table 2]

[0067] [Example 2] Evaluation of moisture retention based on stratum corneum moisture content and water evaporation rate A lotion with the composition shown in Table 3 was prepared using the samples shown in Table 2, and applied to the inner forearms of 10 female subjects in their 40s to 50s twice a day for four consecutive weeks. The moisture content of the stratum corneum was measured using a Corneometer (Courage + Khazaka) before application and 2 and 4 weeks after application, and the amount of transepidermal water loss was measured using a Tewameter (Courage + Khazaka electronic GmbH), respectively. The results are shown in Tables 4 and 5.

[0068] [Table 3]

[0069] [Table 4]

[0070] As shown in Table 4, the moisture content of the stratum corneum was increased in Test Areas 1 to 4 and Control Areas 1 to 3 compared to Comparative Example 4, which used purified water. In particular, all of Test Areas 1 to 4, in which yeast was cultured using birch sap as the substrate, showed an extremely excellent effect on retaining moisture in the stratum corneum, and although some moisturizing effects were observed in the birch sap without yeast culture (Control Area 1), the culture solution with skim milk as the substrate (Control Area 2), and the culture solution with soy milk as the substrate (Control Area 3), the results were inferior to those of Test Areas 1 to 4. In addition, when comparing the types of yeast, it was confirmed that the culture solution of Galactomyces raceii (Test Area 1) and the culture solution of Saccharomycopsis (Test Area 2) showed a high effect on retaining moisture in the stratum corneum.

[0071] [Table 5]

[0072] As shown in Table 5, the amount of water evaporation was increased in control area 2 (purified water) during the test period due to environmental influences, but the other test areas and control areas were confirmed to have a transepidermal water evaporation inhibitory effect. In particular, all test areas 1 to 4, in which yeast was cultured using birch sap as a substrate, had an extremely excellent water evaporation inhibitory effect. Although some water evaporation inhibitory effect was observed in birch sap without yeast culture (control area 1), culture medium with skim milk as a substrate (control area 2), and culture medium with soy milk as a substrate (control area 3), the results were inferior to those of test areas 1 to 4. In addition, when comparing the types of yeast, it was confirmed that the water retention and evaporation inhibitory effect was high in two test areas: birch sap yeast culture medium with Galactomyces raceii selected (test area 1) and birch sap yeast culture medium with Saccharomycopsis selected (test area 2).

[0073] [Example 3] Skin Texture Improvement Test 1 The samples shown in Table 3 were applied twice a day for four weeks to ten female subjects in their 40s and 50s, marking an area of ​​15 mm x 15 mm on the inner forearm of each test area. The skin texture before and after application was photographed using a microscope (Keyence Corporation) and compared. The images were evaluated on the following three items (depth of skin groove, shape of skin mound, size of skin mound) using the following five-point scale, and the average scores of the ten subjects are shown in Tables 6 to 8.

[0074] (1) Depth of the skin groove 5. The skin groove is very shallow. 4. The skin groove is slightly shallow. 3. Skin grooves are normal 2. The skin groove is slightly deep. 1. The skin groove is very deep (2) Shape of the skin mound 5. They are all perfect triangles 4 It is roughly triangular. 3. Skin ridges other than the triangular shape are slightly noticeable 2. Skin ridges other than the triangle are prominent 1. Skin ridges other than the triangle are very noticeable (3) Size of the skin ridge 5. Very small and neat 4. Slightly small 3. Medium size 2. Slightly large 1. Very large

[0075] [Table 6]

[0076] [Table 7]

[0077] [Table 8]

[0078] As shown in Tables 6 to 8, compared to Comparative Example 4, which used purified water, the skin texture improving effect was confirmed in Test Areas 1 to 4 and Control Areas 1 to 3. In particular, all of Test Areas 1 to 4, in which yeast was cultured using birch sap as the substrate, showed an extremely excellent skin texture improving effect. Although some skin texture improving effect was observed in birch sap without yeast culture (Control Area 1), culture solution with skim milk as the substrate (Control Area 2), and culture solution with soy milk as the substrate (Control Area 3), the results were inferior to those of Test Areas 1 to 4. When comparing by type of yeast, it was confirmed that the birch sap yeast culture solution (Test Area 1), which selected Galactomyces raceii, had a high skin texture improving effect.

[0079] [Example 4] Stickiness evaluation test 1 In this Example 4, in the second week of the continuous application test period in Example 2, the stickiness of the application site of each test group before and after drying for 15 minutes was measured and compared using a skin friction force evaluation device, Frictiometer FR700 (manufactured by Courage+Khazaka Co., Ltd.) before and after lotion application. The friction force value before application was set to 100, and the value after application was calculated and averaged for 10 subjects, and is shown in Table 9.

[0080] [Table 9]

[0081] As shown in Table 9, in control area 1, stickiness due to the sugar contained therein was observed, and the frictional force also showed a very high value. In other words, it was clear that birch sap itself gives a sticky feeling. In addition, the yeast culture liquids in control areas 2 and 3, which used skim milk and soy milk as substrates, also showed high frictional force and were sticky, although not as strong as control area 1. In comparison, test areas 1 to 4, in which yeast was cultured using birch sap as a substrate component, showed values ​​close to that of purified water in control area 4, indicating that they had an excellent usability without giving a sticky feeling.

[0082] [Example 5] Skin Texture Improvement Test 2 In this Example 5, the samples shown in Table 10 were applied twice a day to the left and right cheeks of 10 female subjects in their 40s to 50s for 4 weeks. The right cheek was used as the test group and the left cheek was used as the control group. The skin texture before and after application was measured using a skin image analyzer (VISIA™ Evolution, manufactured by Canfield). The skin texture evaluation was quantified and the results are shown in Table 11.

[0083] [Table 10]

[0084] [Table 11]

[0085] As shown in Table 11, the study in this embodiment also confirmed that test area 1, in which Galactomyces was cultivated using birch sap as a substrate component, had a greater effect on improving texture than the control area, in which birch sap itself was used.

[0086] [Example 6] Dullness Improvement Test In this Example 6, in parallel with the test of Example 5, the dullness improving effect was measured in the test section and the control section. The dullness was evaluated using a colorimeter (Konica Minolta), which is a color measuring device, and the lightness index L* value in the L*a*b* color system was obtained and analyzed. The results before application and after 4 weeks of continuous application are shown in Table 12 (in the table, the L* value is the average value of the subjects (10 people)).

[0087] [Table 12]

[0088] As shown in Table 12, it was confirmed that test area 1, in which Galactomyces was cultured using birch sap as a substrate component, had a greater effect of improving dullness than the control area, in which birch sap itself was used.

[0089] [Example 7] Stickiness evaluation test 2 (sensory test) In this Example 7, a sensory test was conducted on the sticky feeling during the tests of Examples 5 and 6. Specifically, the evaluation was conducted every week from 1 week to 4 weeks after the start of the test using the following 5-point scale. The average scores of 10 subjects are shown in Table 13. Skin stickiness evaluation 5: Refreshing 4: Slightly refreshing 3: Normal 2: Slightly sticky 1: Sticky

[0090] [Table 13]

[0091] As shown in Table 13, the test area in which Galactomyces was cultivated using birch sap as the substrate component gave a refreshing feeling throughout the entire period, while the control area in which birch sap itself was used gave a sticky feeling due to the sugars in the sap.

[0092] [Example 8] Example of production of lotion In this Example 8, a lotion (lotion containing birch sap yeast culture fluid) was produced by stirring components (1) to (11) at 80°C according to the recipe in Table 14, dissolving them, and then cooling them to room temperature. A control lotion was also produced in the same manner as in Comparative Example 1, except that the yeast-cultured birch sap prepared in Production Example 1 was not added. All of the obtained lotions were clear, and were stable for 3 months at 40°C and a relative humidity (RH) of 75% without developing any cloudiness.

[0093] [Table 14]

[0094] A sensory test was conducted on these lotions by 10 female subjects in their 40s and 50s. Evaluation was carried out on the following 5-point scale for 4 items (skin moistness, skin smoothness, skin stickiness, and sensory irritation). The average scores of the subjects (10 subjects) are shown in Table 15.

[0095] (1) Moisturizing skin 5: Moisturizing 4: Slightly moist 3: Normal 2: Slightly dry 1: Dryness (2) Skin smoothness 5: Smooth 4: Slightly smooth 3: Normal 2: Slightly rough 1: Rough (3) Sticky skin 5: Refreshing 4: Slightly refreshing 3: Normal 2: Slightly sticky 1: Sticky (4) Sensory stimulation 5: I don't feel any particular irritation. 4: I feel a slight irritation, but it doesn't bother me. 3: There is some irritation such as a tingling, burning sensation, prickling sensation, or itchiness, but it is tolerable. 2: Strong irritation such as tingling, burning, prickling, and itching. 1: Very strong irritation such as tingling, burning, prickling, and itching. 0: Severe stimulation, cannot continue testing

[0096] [Table 15]

[0097] As shown in Table 15, the lotion containing the culture solution of Galactomyces cultured using birch sap as a substrate component exhibited superior moisturizing properties and reduced stickiness compared to the control lotions 1 and 2.

[0098] [Example 9] Preparation of cream In this Example 9, according to the formulation shown in Table 16, a cream was produced by mixing and stirring components (1) to (7) at 80°C and adding a mixture obtained by mixing and stirring components (8) to (11) at 80°C separately to the mixture obtained by mixing and stirring the components (1) to (7) at 80°C. The mixture was homogenized and cooled to room temperature with stirring to produce a cream.

[0099] The cream obtained was not sticky during use and moisturized the skin.

[0100] [Table 16]

[0101] [Example 10] Preparation of body gel In Example 10, a body gel was prepared by stirring and dissolving the components (1) to (7) according to the recipe in Table 17. The obtained body gel moisturized the skin and was a non-irritating preparation.

[0102] [Table 17]

[0103] [Example 11] Preparation of shampoo In Example 11, components (1) and (2) were dissolved by heating according to the recipe in Table 18, and components (3) and (4) heated to 60°C were added and stirred. Components (5) to (8) were added and stirred. Component (9) was added to adjust the pH to 5.5 to 6.0, and further components (11) and (12) dissolved in component (10) were added. Component (13) was added until the total amount (100%) was reached to produce a shampoo.

[0104] When the hair was washed with the obtained shampoo, the hair felt smooth and moisturized.

[0105] [Table 18]

[0106] [Example 12] Preparation of cleansing cream In Example 12, a cleansing composition was obtained by homogeneously mixing all ingredients by stirring according to the formulation in Table 19. The resulting cleansing cream had good cleansing properties and provided a refreshing feeling after use.

[0107] [Table 19]

[0108] [Example 13] Preparation of rinse agent In Example 13, a rinse composition was obtained by homogeneously mixing all the components by stirring according to the formulation in Table 20. The obtained rinse gave hair and scalp a moist feeling after use.

[0109] [Table 20]

[0110] [Example 14] Preparation of emulsion In Example 14, a milky lotion composition was obtained by homogeneously mixing all the ingredients by stirring according to the formulation in Table 21. The obtained milky lotion provided a moist and moisturizing feeling to the skin.

[0111] [Table 21]

[0112] [Example 15] Mask manufacturing In Example 15, a mask was prepared by impregnating a nonwoven fabric with a skin topical preparation having the composition shown in Table 22. The prepared mask was used to cover the face for 15 minutes, and provided a suitable level of moisture and firmness after use.

[0113] [Table 22]

Claims

1. A culture medium in which a yeast belonging to the genus Galactomyces and / or a yeast belonging to the genus Saccharomycopsis is grown in a medium containing birch sap.

2. 2. The culture medium according to claim 1, characterized in that the birch sap is sap obtained from Betula platyphylla.

3. A skin external preparation comprising the culture medium according to claim 1 or 2.

4. A skin cosmetic comprising the culture medium according to claim 1 or 2.

5. 5. The skin cosmetic composition according to claim 4, which exhibits at least one effect selected from the group consisting of an effect of improving moisturizing properties, an effect of improving skin texture, and an effect of reducing dullness.

6. A method for producing a culture medium, characterized in that during the process of culturing a yeast belonging to the genus Galactomyces and / or a yeast belonging to the genus Saccharomycopsis in a medium containing birch sap, low molecular weight sugars in the birch sap are consumed as a nutrient source for the fungi, resulting in a lower concentration of low molecular weight sugars in the birch sap compared to before the start of the culture.

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