Adipose tissue regeneration substrate

Abstract

The purpose of the present invention is to provide an adipose tissue regeneration base material capable of regenerating a large volume of adipose tissue which has a normal shape and good handleability. The present invention is an adipose tissue regeneration base material composed of: a particulate matter made from a bioabsorbable material that has an internal space and has, on the surface thereof, a plurality of openings communicating with the internal space; and a bag-shaped matter made from a bioabsorbable material that has an opening and encloses a plurality of said particulate matters.

Description

[Technical field]

[0001] The present invention relates to an adipose tissue regeneration substrate that is easy to handle and capable of regenerating a large volume of adipose tissue in a normal shape. [Background technology]

[0002] In breast cancer treatment, when cancer in the breast is difficult to cure with radiation or chemotherapy alone, surgery is used to remove the cancer. In the past, total mastectomy, in which the entire breast including the diseased tissue was removed, was common, but in recent years, improvements in testing technology have allowed for early detection of small disease lesions, making it possible to perform breast-conserving surgery, in which only the tissue is removed. However, even with breast-conserving surgery, the resected area still becomes sunken, which still places a mental burden on the patient. For this reason, an increasing number of patients are undergoing breast reconstruction surgery after surgical treatment in order to improve their quality of life.

[0003] Silicone implants are commonly used in breast reconstruction surgery, but because they are not bioabsorbable, they remain in the body forever as foreign bodies, which can lead to postoperative leakage or infection due to foreign body reactions. There are also concerns about adverse effects such as allergies and carcinogenesis upon contact.

[0004] Another method is to harvest adipose tissue from another part of the body and transplant it to the affected area, but the tissue is quickly absorbed after transplantation, causing the depression to reoccur. Also, harvesting tissue creates a new wound, which is not necessarily desirable from the perspective of quality of life.

[0005] In order to solve these problems of conventional breast reconstruction surgery, the inventors have disclosed a breast reconstruction component in which a sponge containing collagen is encapsulated inside hollow granules made of polylactic acid (Patent Document 1). The breast reconstruction component of Patent Document 1 is filled into the space created by partial breast resection, and surrounding cells invade the breast reconstruction component and grow using the breast reconstruction component as a scaffold, making it possible to reconstruct the breast without transplanting adipose tissue from another site. In addition, since the breast reconstruction component of Patent Document 1 is made of a bioabsorbable material, it is gradually absorbed into the body as the regeneration of the breast progresses and eventually disappears, making it highly safe. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] JP 2016-140494 A Summary of the Invention [Problem to be solved by the invention]

[0007] The breast reconstruction component of Patent Document 1 is very effective as a breast reconstruction technique because it can safely regenerate a breast made of living own cells. However, the breast reconstruction component of Patent Document 1 has a problem that it is less easy to implant because a large amount of breast reconstruction components must be packed when the resection area is large. In particular, as a step in breast cancer treatment, radiation therapy is used in combination with cancer resection to completely kill cancer cells. As a result, the skin hardens, so the skin is gradually enlarged using a tissue expander or the like, then incised, the tissue expander is removed, and the implant is inserted. In fact, to implant a large number of small implants as in Patent Document 1, a large incision is required to implant them cosmetically, which increases the burden on the patient.

[0008] To solve this problem, it was considered to increase the size of the breast reconstruction components, but if the size of the breast reconstruction components is too large, the moldability decreases, and it becomes difficult to regenerate fatty tissue to the center, which reduces the tissue regeneration performance.In addition, even if a large number of breast reconstruction components are inserted, each breast reconstruction component can move due to changes in posture or external forces, and the shape of the implanted part is easily distorted, making it difficult to regenerate a breast with a beautiful shape.

[0009] An object of the present invention is to provide an adipose tissue regeneration substrate that is easy to handle and capable of regenerating a large volume of adipose tissue in a normal shape. [Means for solving the problem]

[0010] The present invention is an adipose tissue regeneration base material composed of a granular body made of a bioabsorbable material having an internal space and a plurality of openings on its surface that lead to the internal space, and a bag-shaped body made of a bioabsorbable material having openings and encasing the plurality of granular bodies. The present invention will be described in detail below.

[0011] As a result of extensive research, the inventors discovered that by packing multiple granules made of bioabsorbable material into a bag-shaped body made of bioabsorbable material with an opening, the body can be easily implanted even when the excision site is large, and the shape is less likely to collapse even when external force is applied, making it possible to regenerate tissue of normal shape, thereby completing the present invention.

[0012] The adipose tissue regeneration substrate of the present invention is composed of a granular body made of a bioabsorbable material having an internal space and a plurality of openings on its surface leading to the internal space, and a bag-shaped body made of a bioabsorbable material having openings and encasing the plurality of granular bodies. Schematic diagrams of the adipose tissue regeneration base material of the present invention and the above-mentioned granules are shown in FIGS. As shown in FIG. 1, the adipose tissue regeneration substrate of the present invention has a structure in which a plurality of granules 1 are enclosed inside a bag-shaped body 3. The granules 1 have an internal space, and have a plurality of openings on the surface that lead to the internal space, and are generally closed. The bag-shaped body 3 has a number of openings and an internal space, and is closed so that the contents enclosed inside do not move to the outside. The cells that pass through the openings of the bag-shaped body 3 and the granules 1 grow using the wall surface inside the granules as a scaffold to regenerate the adipose tissue. In addition, since the granules 1 and the bag-shaped body 3 are made of a bioabsorbable material, they maintain the space of the adipose tissue to be regenerated until the adipose tissue is regenerated, and after the adipose tissue is regenerated, they are absorbed into the body and eventually disappear. Since the adipose tissue regeneration substrate of the present invention is made of a plurality of granules bound together by a bag-shaped body, it is easy to embed in a large space and is highly easy to handle. In addition, since the plurality of granules 1 are wrapped in the bag-shaped body 3, the granules 1 do not scatter over a wide area, and therefore it is possible to embed the granules in a shape close to the shape after regeneration. Furthermore, even if an external force is applied after implantation, the granules 1 do not move outward from the bag-like bodies 3, so the shape at the time of implantation is unlikely to be distorted, and adipose tissue of a normal shape can be regenerated. Furthermore, the adipose tissue regeneration substrate of the present invention may have a sponge-like porous body 2 made of a bioabsorbable material provided inside the granules 1. Providing a sponge-like porous body 2 made of a bioabsorbable material inside the granules 1 increases the scaffolding for cells, thereby further promoting the regeneration of adipose tissue and increasing its strength. The sponge-like porous body includes not only a spongy body but also a body having a large number of voids such as nonwoven fabric and cotton.

[0013] The bioabsorbable material constituting the granules is not particularly limited as long as its safety as an implant has been confirmed, but since adipose tissue regeneration takes about six months to a year, it is preferable that the material has the strength and decomposition rate to maintain the space in which the adipose tissue regeneration substrate is embedded during that period. Examples of such bioabsorbable materials include natural polymers such as collagen, gelatin, chitin, and chitosan, and synthetic polymers such as homopolymers of lactic acid, glycolic acid, ε-caprolactone, dioxanone, and trimethylene carbonate, or copolymers of at least two or more materials selected from these. Among these, polylactic acid or copolymers of lactic acid and other bioabsorbable materials are preferred because their strength and decomposition rate in vivo are suitable for adipose tissue regeneration substrates. Examples of the copolymers of polylactic acid or lactic acid and other bioabsorbable materials include polylactide, copolymers of lactide and glycolic acid, and copolymers of lactide and ε-caprolactone described in Patent Document 1.

[0014] When the granular bioabsorbable material is polylactide, a copolymer of lactide and glycolic acid, or a copolymer of lactide and ε-caprolactone, the weight-average molecular weight is preferably 4000 to 300000. By setting the weight-average molecular weight within the above range, the decomposition rate can be made more suitable for the regeneration of adipose tissue. The weight-average molecular weight is more preferably 100000 or more, and more preferably 200000 or less.

[0015] The shape of the granular body is not particularly limited as long as it can provide a scaffold for cell proliferation and maintain the space of the adipose tissue to be regenerated, and examples of such shapes include spherical, columnar, irregular, etc. Among these, a spherical shape is preferable, and an ellipsoidal spheroid is more preferable, since the shape is unlikely to be distorted by an external force after implantation, and appropriate gaps are generated between the granular bodies, which can further promote the regeneration of adipose tissue.

[0016] The size of the internal space is not particularly limited, but is preferably 10 mm 3 More than 100000mm 3The size of the internal space is preferably 25 mm or less. By setting the size of the internal space within the above range, it is possible to regenerate the adipose tissue to the center of the granule more reliably while securing a space for regenerating the adipose tissue. The size of the internal space is preferably 25 mm or less. 3 More preferably, it is 50 mm or more. 3 More preferably, it is 50,000 mm or more. 3 More preferably, it is 25,000 mm or less. 3 It is even more preferable that:

[0017] The shape of the openings of the granular material is not particularly limited, and may be circular, lattice-like, polygonal, irregular, or the like. The number of openings of the granular body is not particularly limited as long as it is two or more. The size and occupancy of the openings of the granular body are not particularly limited as long as the cells can smoothly pass into the inside of the granular body, but it is preferable that the openings having a maximum length of 0.1 mm or more and 20 mm or less are distributed at an occupancy rate of 50% to 99% of the surface area of ​​the granular body. The size and occupancy of the openings in the above range can further improve the balance between the strength of the granular body and the invadibility of the cells. The maximum length of the openings may be a size that allows adipose tissue to invade and does not allow surrounding tissues other than fat that already exist as tissues to invade, and is more preferably 15 mm or less, and even more preferably 10 mm or less. The occupancy rate of the openings is more preferably 60% or more of the surface area of ​​the granular body to facilitate tissue infiltration, and more preferably 70% or more, and more preferably 95% or less from the viewpoint of ensuring the shape of the granular body, and even more preferably 90% or less. In this specification, the maximum length refers to the maximum length when the distance between two points of the openings is measured.

[0018] More specific examples of the granules include mesh granules, porous capsules, etc. When the granules are mesh granules, the mesh constituting the granules includes a net, woven fabric, knitted fabric, etc., formed from monofilaments or multifilaments. Among them, mesh ellipsoids are more preferable from the viewpoints of elasticity, shape retention, adipose tissue penetration, etc.

[0019] When the granular material is made of a mesh, the thickness of each mesh constituting the granular material is not particularly limited, but is preferably 0.05 mm to 1 mm, more preferably 0.1 mm to 0.4 mm, from the viewpoints of mesh elasticity, shape retention, cell penetration, etc. The size of the mesh of the granular material is preferably in the range of 0.01 mm to 6 mm in both length and width, more preferably 0.02 mm to 5 mm.

[0020] The size of the granules is not particularly limited, but when the granules are elliptical, the major axis is preferably 8 mm to 150 mm, and the minor axis is preferably 5 mm to 100 mm. By making the size of the granules within the above range, it is possible to easily adjust the shape when regenerating a large volume of adipose tissue, and to more reliably regenerate adipose tissue to the center. The major axis of the granules is more preferably 10 mm to 30 mm, and even more preferably 15 mm to 20 mm. The minor axis of the granules is more preferably 5 mm to 20 mm, and even more preferably 7 mm to 15 mm.

[0021] The number of the above-mentioned granules in the adipose tissue regeneration substrate of the present invention is not particularly limited as long as it is two or more, and can be adjusted appropriately depending on the size of the granules and the size of the space in which they are to be implanted. From the standpoint of ease of handling and further promoting the regeneration of large volumes of adipose tissue, however, the number is preferably five or more, more preferably ten or more, and preferably 100 or less, and more preferably 50 or less.

[0022] The bioabsorbable material constituting the sponge-like porous body is not particularly limited, and examples thereof include synthetic polymers such as polyglycolide, polylactide, poly-ε-caprolactone, lactide-glycolic acid copolymer, glycolide-ε-caprolactone copolymer, lactide-ε-caprolactone copolymer, polycitric acid, polymalic acid, poly-α-cyanoacrylate, poly-β-hydroxy acid, polytrimethylene oxalate, polytetramethylene oxalate, polyorthoester, polyorthocarbonate, polyethylene carbonate, poly-γ-benzyl-L-glutamate, poly-γ-methyl-L-glutamate, poly-L-alanine, and polyglycol sebacic acid, and natural polymers such as polysaccharides such as starch, alginic acid, hyaluronic acid, chitin, pectinic acid, and derivatives thereof, and proteins such as gelatin, collagen, albumin, and fibrin. Among these, it is preferable to contain collagen because of its high affinity with the living body.

[0023] When the sponge-like porous body contains collagen, it preferably contains 50% or more by weight of collagen, more preferably 60% or more by weight, even more preferably 70% or more by weight, even more preferably 80% or more by weight, particularly preferably 90% or more by weight, very preferably 95% or more by weight, and most preferably 100% by weight.

[0024] The above-mentioned collagen can be used without any particular limitation from collagen derived from skin, tendons, etc. of cows, pigs, etc. Among them, from the viewpoint of eliminating antigenicity and enhancing safety, atelocollagen from which telopeptides have been removed as much as possible by treating collagen with an enzyme such as protease or pepsin is preferred.

[0025] Commercially available collagen-containing sponge-like porous bodies include, for example, Pernac (manufactured by Smith & Nephew Management Co.), Terudermis (manufactured by Terumo Corporation), and the like.

[0026] The present invention also relates to a granular material used in the adipose tissue regeneration base material of the present invention as described above, which is made of a bioabsorbable material having an internal space and a plurality of openings on its surface that lead to the internal space.

[0027] The shape of the bag-like body is not particularly limited, and any shape can be used depending on the ease of shaping at the implantation site, such as a rectangular bag-like shape, a circular bag-like shape, etc. Specific embodiments include a net woven from filaments into a bag-like shape, a porous bag-like body, etc.

[0028] The bioabsorbable material constituting the bag-like body is not particularly limited, and since it does not need to maintain its strength for a long period of time compared to granular bodies, it can be the same as the bioabsorbable material constituting the sponge-like porous body. However, it needs to be strong enough to hold a plurality of granular bodies and maintain its overall shape, and since it is to be embedded in a living body as an implant, it is preferable that it is a material that causes as little inflammatory or foreign body reaction as possible. Such bioabsorbable materials include materials that can be used as sutures, and it is preferable to use polyglycolide, polylactic acid, polycaprolactone, polydioxane, trimethylene carbonate, or copolymers thereof, and it is more preferable to use polyglycolide, copolymers of polyglycolide and other bioabsorbable materials, or copolymers of lactic acid and other bioabsorbable materials.

[0029] When the bag-shaped body is a net, the thickness of the filaments constituting the bag-shaped body is not particularly limited, but from the viewpoint of the balance between flexibility and strength, it is preferably 0.01 mm or more, more preferably 0.1 mm or more, preferably 2 mm or less, and more preferably 0.5 mm or less.

[0030] The occupancy rate of the opening of the bag-like body is not particularly limited as long as the cells can smoothly pass through the granules, but is preferably 50% or more and 99% or less of the surface area of ​​the bag-like body. With an occupancy rate of the opening in the above range, the balance between the strength of the bag-like body and the invadibility of the cells can be further improved. The occupancy rate of the opening of the bag-like body is more preferably 60% or more of the surface area of ​​the bag-like body, even more preferably 70% or more, more preferably 95% or less, and even more preferably 90% or less.

[0031] The size of the opening of the bag-like body is not particularly limited as long as it does not inhibit the intrusion of cells into the granules and the granules do not protrude outside the bag-like body, but the maximum length of the opening of the bag-like body is preferably 1 / 50 times or more of the short axis of the granules, more preferably 1 / 20 times or more, preferably 1 / 3 times or less, and more preferably 1 / 10 times or less. By setting the size of the opening of the bag-like body within the above range, the shape imparting ability and operability of the entire adipose tissue regeneration substrate obtained can be further improved.

[0032] When the bag-like body is a net, the specific numerical values ​​of the mesh size of the bag-like body are, for example, preferably 0.02 mm or more and 0.5 mm or less in both length and width, and more preferably 0.05 mm or more and 0.1 mm or less.

[0033] The size of the bag-like body can be adjusted appropriately depending on the volume of the implantation site and the number of the granules, but from the viewpoint of increasing the moldability of the adipose tissue regeneration base material while suppressing the collapse of the granules after implantation, the internal space of the bag-like body is preferably 1.2 times or more, more preferably 1.5 times or more, and preferably 3 times or less, and more preferably 2 times or less of the total volume of the granules. Note that the total volume of the granules includes the volume of the internal space of the granules.

[0034] The method for producing the adipose tissue regeneration substrate of the present invention is not particularly limited, and for example, the substrate can be produced by wrapping the sponge-like porous body with a mesh made of a bioabsorbable material and closing the ends to produce a plurality of granular bodies, and then wrapping the resulting granular bodies in a bag-shaped body made of a bioabsorbable material and closing the ends. It is also possible to insert the sponge-like porous body material from the opening after producing the granular bodies. The method for closing the ends of the mesh or bag-shaped body is not particularly limited, and examples include a method of tying filaments together and heat compression bonding.

[0035] The adipose tissue regeneration substrate of the present invention is used for the purpose of regenerating adipose tissue by embedding it in adipose tissue. By using the present invention, it is possible to regenerate living adipose tissue consisting of the patient's own cells without embedding tissue from another site. Examples of adipose tissue for which the present invention can be used include breasts, buttocks, and abdomen. In particular, since the present invention can regenerate a large volume of adipose tissue in a normal shape, it is highly effective for the purpose of regenerating a breast by embedding it in a defect caused by partial resection of the breast. Effect of the Invention

[0036] According to the present invention, it is possible to provide an adipose tissue regeneration substrate that is easy to handle and capable of regenerating a large volume of adipose tissue in a normal shape. [Brief description of the drawings]

[0037] [Figure 1] FIG. 1 is a schematic diagram of the adipose tissue regeneration substrate of the present invention. [Diagram 2] FIG. 2 is a schematic diagram of a granular body. [Diagram 3] 1 is a graph showing the measurement results of formability with respect to hole-like defects. [Figure 4] 1 is a graph showing the results of measuring formability for horizontal defects. [Diagram 5] 1 shows nuclear magnetic resonance images (MRI) taken 0 (immediately after implantation), 1, 3, 6, and 9 months after implantation of the adipose tissue regeneration scaffold obtained in Example 1 into a porcine fascial defect. [Figure 6]1 shows a hematoxylin and eosin (HE) stained image of the transplanted area 6 months after the adipose tissue regeneration scaffold obtained in Example 1 was transplanted into a porcine epifascial defect. [Figure 7] 1 shows an Oil Red O stained image of the transplanted area 6 months after the adipose tissue regeneration scaffold obtained in Example 1 was transplanted into a porcine fascial defect. [Figure 8] 1 shows an Azan stained image of the transplanted area 6 months after the adipose tissue regeneration scaffold obtained in Example 1 was transplanted into a porcine fascial defect. [Figure 9] 1 shows an anti-CD31 antibody immunostained image of the transplant site 6 months after the adipose tissue regeneration scaffold obtained in Example 1 was transplanted into a porcine fascial defect. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0038] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0039] Example 1 A collagen sponge (Pelnac, Smith & Nephew Wound Management) was wrapped in a mesh (filament thickness: 0.2mm-0.25mm, mesh opening: 1x1mm-2x2mm) made of polylactic acid (weight average molecular weight: 220,000) and the ends were closed by heat compression to obtain elliptical granules with a major axis of 18mm and a minor axis of 7.5mm containing a collagen sponge inside. Next, 30 granules were produced in the same manner, and the resulting granules were wrapped in an envelope-shaped bag-shaped body (filament composition: 0.015mm x 12, mesh size: 0.05mm x 0.05mm) made of polyglycolide multifilament with a size of 110mm x 35mm, and the ends were closed by heat welding to obtain an adipose tissue regeneration matrix.

[0040] Example 2 An adipose tissue regeneration scaffold was obtained in the same manner as in Example 1, except that a collagen sponge was not used.

[0041] Comparative Example 1 Thirty granules of Example 1 were used as they were as a base material for adipose tissue regeneration.

[0042] <Evaluation> The adipose tissue regeneration scaffolds obtained in the Examples and Comparative Examples were evaluated as follows.

[0043] (Evaluation of tissue regeneration 1) A subcutaneous incision was made in the back of a miniature pig (approximately 20 kg), and the adipose tissue regeneration materials obtained in Examples 1 and 2 were implanted on the left side of the midline. After 4 months, the area where the adipose tissue regeneration material was implanted was removed, and the presence or absence of tissue regeneration was confirmed. Approximately 4 cm of tissue was regenerated.

[0044] (Evaluation of tissue regeneration 2) A large miniature pig (approximately 25 kg) was prepared as an experimental animal, and a midline incision was made in the abdominal skin. Next, the fat and mammary tissues on the left and right sides of the abdomen were removed, and a defect was created under the mammary gland and on the fascia. The adipose tissue regeneration scaffold obtained in Example 1 was transplanted into the defect on the fascia, and the skin was sutured. After surgery, abdominal magnetic resonance images (MRI) were taken at 0 (immediately after transplantation), 1, 3, 6, and 9 months. The MRI images are shown in Figure 5. Six months after the operation, the adipose tissue above the muscle layer on the right side of the abdomen was removed, and the transplanted area was excised. The obtained specimen was cut into sections and stained with hematoxylin and eosin (HE), oil red O, azan, and anti-CD31 antibody immunostained. The microscopic images of each stain are shown in Figures 6, 7, 8, and 9, respectively. As shown in Figure 5, 6 months after surgery, regeneration of adipose tissue from the surrounding area in contact with adipose tissue and mammary gland tissue was observed in the area where the adipose tissue regeneration scaffold was implanted (areas colored white in the MRI image (T1 weighted image) in Figure 5). Furthermore, 9 months after surgery, regeneration of adipose tissue from the periphery of the adipose tissue regeneration scaffold was observed over a larger area. In addition, the formation of adipose tissue and collagen tissue was observed inside the adipose tissue regeneration scaffold 6 months after surgery, as shown in Figures 6, 7, and 8. Furthermore, the formation of blood vessels was observed in the adipose tissue and collagen tissue, as shown in Figure 9.

[0045] (Evaluation of moldability) (1) Formability for hole-like defects 358 g of chicken breast meat with skin was prepared as a substitute for skin and adipose tissue, and the skin was partially peeled off to expose the meat. Next, a cross was made in the exposed meat, and the center was hollowed out to create a hole-shaped defect. After the skin was returned to its original position, the length (length, width) of the incision and the height of the defect were measured. Then, the adipose tissue regeneration scaffold obtained in Example 1 was embedded in the defect, and the skin was returned to its original position, and the length (length, width) of the incision and the height of the defect were measured. Next, the length of the incision (length, width) and the height of the defect were measured in the same manner using 30 pieces of adipose tissue regeneration substrate from Comparative Example 1. The measurement results are shown in Figure 3. From the measurement results, it can be seen that in Comparative Example 1, the adipose tissue regeneration substrate penetrates into the incision site and is difficult to mold in the height direction, whereas in Example 1, the adipose tissue regeneration substrate does not spread much in the length and width directions and is densely packed in the height direction to form a mountain, making it easy to mold a tall shape and providing excellent moldability for adipose tissue in the breasts, buttocks, etc.

[0046] (2) Formability for horizontal defects 379 g of chicken breast meat with skin was prepared as a substitute for skin and adipose tissue, and the skin was partially peeled off to expose the meat. Next, a single incision was made in the direction of the muscle fibers of the exposed meat, and the length (horizontal), length (vertical) when the incision was opened, and height of the incision were measured. Then, the adipose tissue regeneration substrate obtained in Example 1 was embedded in the incision, and the length (vertical, horizontal) and height of the incision were measured. Next, the length (vertical, horizontal) and height of the incision were measured using 30 pieces of the adipose tissue regeneration substrate of Comparative Example 1 in the same manner. At this time, when the adipose tissue regeneration substrates of Example 1 and Comparative Example 1 were observed after implantation, the adipose tissue regeneration substrate did not protrude from the incision in Example 1, but multiple pieces of the adipose tissue regeneration substrate protruded from the incision and fell off in Comparative Example 1. Note that the measurement of Comparative Example 1 was performed after the protruding and fallen adipose tissue regeneration substrate was pushed back into the incision. The measurement results are shown in Figure 4. From the measurement results, it can be seen that in Comparative Example 1, the adipose tissue regeneration base material spreads in the vertical direction of the incision, making it difficult to mold in the height direction, whereas in Example 1, it does not spread easily in the vertical or horizontal directions and is densely packed in the height direction to form a mountain, making it easy to mold a tall shape and providing excellent moldability for adipose tissue in the breasts, buttocks, etc. [Industrial Applicability]

[0047] According to the present invention, it is possible to provide an adipose tissue regeneration substrate that is easy to handle and capable of regenerating a large volume of adipose tissue in a normal shape. [Explanation of symbols]

[0048] 1 Granules 2. Sponge-like porous body 3 Bag-like body

Claims

1. A granular body made of a bioabsorbable material having an internal space and a plurality of openings on the surface thereof that communicate with the internal space; a bag-shaped body made of a bioabsorbable material having a plurality of openings and enclosing a plurality of the granular bodies; The granular material has a sponge-like porous body made of a bioabsorbable material inside a mesh having a generally closed shape, The bag-shaped body is a bag-shaped net, the mesh comprises polylactic acid or a copolymer of lactic acid and other bioabsorbable materials; The sponge-like porous body contains at least one natural polymer selected from the group consisting of collagen, gelatin, albumin, and fibrin. Adipose tissue regeneration base material.

2. The adipose tissue regeneration material according to claim 1 , wherein the granules are ellipsoidal.

3. 3. The adipose tissue regeneration matrix according to claim 1 or 2, wherein the bioabsorbable material constituting the bag-shaped body is polyglycolide, a copolymer of polyglycolide and another bioabsorbable material, or a copolymer of lactic acid and another bioabsorbable material.

4. 4. The adipose tissue regeneration matrix according to claim 1, 2 or 3, which is used by being implanted in a defect caused by partial mastectomy.

Images