Personal dental care products to prevent demineralization

Abstract

The present invention provides a novel dental treatment product comprising a self-assembling peptide capable of self-assembly at a specific pH, which is useful for dental treatment, particularly for reducing or preventing demineralization of a subject's teeth, particularly for reducing or preventing further demineralization of a subject's teeth. The dental treatment product comprises a self-assembling peptide, particularly a self-assembling peptide comprising the sequence of SEQ ID NO: 21, capable of self-assembly at a pH of less than 7.5, and a pharmaceutically acceptable base, wherein the self-assembling peptide is essentially present in the dental treatment product in an assembled form. The dental treatment product is an essentially solid product selected from the group consisting of chewing gum, soft chew, taffy, gelatin gum, chewing candy, chewing gum, and lozenges. Preferably, it is a chewy product. The dental treatment product is not abrasive. The dental treatment product is useful for reducing or preventing (further) demineralization of the tooth surface of a subject with demineralized teeth, for example, a subject with xerostomia, hyposalivation, bruxism, gastroesophageal reflux disease, dentin hypersensitivity, and / or acid erosion. Preferably, it is also useful for cleaning the surface of teeth. The products of the present invention are useful for animals and humans. The present invention also provides a method for preparing the dental care products of the present invention. The present invention allows for non-targeted treatment of multiple teeth, which is independent of caries diagnosis.

Description

[Technical field]

[0001] The present invention provides a new dental treatment product comprising a self-assembling peptide that can undergo self-assembly at a specific pH, which is useful for dental treatment, particularly for reducing or preventing the demineralization of the teeth of a subject, particularly for reducing or preventing further demineralization of the teeth of a subject.The dental treatment product comprises a self-assembling peptide, particularly a self-assembling peptide comprising the sequence of SEQ ID NO: 21 that can undergo self-assembly at a pH of less than 7.5, and a pharma- ceutically acceptable base, wherein the self-assembling peptide is essentially present in the dental treatment product in an assembled form.The dental treatment product is an essentially solid product selected from the group consisting of chewing gum, soft chew, taffy, gelatin gum, chewing candy, chew toy, marshmallow, lozenge, or tablet.Preferably, it is a chewy product.The dental treatment product is not abrasive. The dental care product is useful for reducing or preventing (further) demineralization of the tooth surface of subjects with demineralized teeth, such as subjects with xerostomia, hyposalivation, bruxism, gastroesophageal reflux disease, dentin hypersensitivity and / or acid erosion. Preferably, in these diseases, a layer of self-assembling peptides is formed on the tooth surface, which may be eroded or demineralized, i.e., acts as a sacrificial layer before the tooth itself is damaged. Preferably, the dental care product of the present invention is also useful for cleaning the tooth surface. The product of the present invention is useful for animals and humans. The present invention also provides a process for preparing the dental care product of the present invention. The present invention allows for non-targeted treatment of multiple teeth, which is independent of the diagnosis of caries. [Background technology]

[0002] Tooth demineralization is the removal of minerals (mainly calcium and phosphate) from hard tissues such as enamel, dentin, and cementum. It begins at the surface and may progress to the development of cavities unless arrested or reversed by remineralization.

[0003] Demineralization is caused by bacteria excreting acids as a product of carbohydrate metabolism. Reducing the frequency of carbohydrate in an individual's diet can increase remineralization and decrease demineralization. If the demineralization phase continues to outweigh the remineralization phase for an extended period of time, loss of tooth enamel structure and cavitation may occur. This disruption of the remineralization / demineralization equilibrium caused by the presence of fermentable carbohydrates continues until the saliva has returned to normal pH, long enough to penetrate and neutralize the acids in any cariogenic biofilm present (Arathi Rao, et al. 2011. The Role of Remineralizing Agents in dentistry: A Re-view. Volume 32, Number 6; Wikipedia on Remineralisation_of_teeth#Treatment_and_prevention).

[0004] In addition to bacterial invasion, enamel is also susceptible to other destructive forces. Bruxism, also known as teeth clenching or grinding, destroys enamel very quickly. The rate of wear of enamel, called attrition, is 8 micrometers per year from a normal factor. A common misconception is that enamel wears off mostly from chewing, when in reality teeth rarely touch during normal chewing. Moreover, normal tooth contact is physiologically compensated for by the periodontal ligament and the occlusal arrangement of the teeth. The truly destructive forces are dysfunctional movements, such as those found in bruxism, which can cause irreversible damage to enamel.

[0005] Other non-bacterial processes of enamel destruction include abrasion (including foreign bodies such as toothbrushes), erosion (including chemical processes such as dissolution by soft drinks or lemon and other juices) (Larsen MJ et al. 1999. Enamel erosion by some soft drinks and orange juices relative to their pH, buffering effect and contents of calcium phosphate. Caries Res. 33 (1): 81-87), and possibly abfraction (including compressive and tensile forces). Gastroesophageal reflux disease can also result in erosive enamel loss as acid refluxes up the esophagus into the mouth, most often occurring during a night's sleep (Wikipedia on Tooth_enamel#Enamel_loss).

[0006] Acid erosion is a type of tooth wear. It is defined as the irreversible loss of tooth structure due to chemical dissolution by acids not of bacterial origin. Although acid erosion is the most common chronic disease in children aged 5-17 years, it has only recently been recognized as a dental health problem. The harmful effects of acid erosion are generally not widely known, and this is especially true in the case of erosion caused by fruit juices, because fruit juices tend to be viewed as healthy. Acid erosion begins in the enamel, thinning it and can progress into the dentin, giving the tooth a dull yellow appearance and resulting in dentin hypersensitivity.

[0007] The most common cause of erosion is acidic foods and beverages. In general, foods and beverages with a pH below 5.0-5.7 are known to cause the effects of acid erosion. Numerous clinical and laboratory reports link erosion to excessive consumption of beverages. Those thought to pose a risk are soft drinks, some alcoholic and fruit drinks, fruit juices such as orange juice (which contain citric acid), and carbonated drinks such as cola (the carbonation is not the cause of erosion, but the citric and phosphoric acids). Additionally, wine has been shown to erode teeth, and has a low pH of 3.0-3.8. Other possible sources of corrosive acid are exposure to chlorinated pool water, and gastric acid reflux. In children with chronic diseases, the use of medications containing acidic ingredients is also a risk factor. Acid erosion has also been recorded in the fossil record and may have been caused by the consumption of acidic fruits or plants. (Wikipedia on acid erosion).

[0008] To prevent demineralization in the mouth, it is important to have a balanced diet, including foods containing calcium and foods that are low in acid and sugar. Fluoride is also thought to prevent demineralization because its incorporation into enamel results in the production of fluoridated hydroxyapatite, which has improved resistance to acid. Fluoride may be administered in dental care products, such as toothpaste, or in foods or beverages; for example, in some areas it is added to drinking water.

[0009] In healthy subjects, there is a balance between demineralization and remineralization that maintains dental health. Thus, there are many strategies to combat demineralization that are based on increasing tooth remineralization.

[0010] To date, tooth remineralization is primarily achieved by delivering either fluoride or calcium and phosphate ions onto dental lesions or cavities (Arifa et al., Int J Clin Pediatr Dent 12(2): 139-144). Calcium and phosphate ions are typically included in toothpastes, which also contain, for example, abrasives, fluoride, surfactants, and other remineralization agents. Calcium and phosphate ions may be used in various crystalline forms, for example, as hydroxyapatite-based materials, or as amorphous calcium phosphates, such as some casein phosphopeptide-based materials. For example, WO 2013 / 050432 describes such remineralization agents and options for contacting mucosa with these agents. WO 2009 / 100276 teaches dental flosses combined with basic amino acids, in free or salt form, for example, to promote remineralization.

[0011] Recently, an alternative approach to tooth remineralization has been described, based on short rationally designed self-assembling peptides. WO2004 / 007532 discloses peptides capable of forming three-dimensional scaffolds, thereby promoting the nucleation of de novo calcium phosphates. These artificial peptides assemble to form beta-sheet, tape-like assemblies. The peptide assemblies can be switched from a fluid to a nematic, stiffer gel state in response to a chemical or physical trigger. The peptides were designed to form assemblies in a hierarchical order of tapes, ribbons, fibrils, and fibers in response to specific pH and / or ionic strength. Aggeli et al. (2003, J. Am. Chem. Soc. 125, 9619-9628) analyze pH as a trigger for the self-assembly of peptide beta-sheets.

[0012] Several other self-assembling peptides have been described in the prior art. For example, WO2010 / 041636A1 describes a bioadsorbable peptide tissue occluding agent containing an artificial peptide having 8-200 amino acid residues with alternating hydrophilic and hydrophobic amino acids, which self-assembles into a beta structure at physiological pH. Self-assembling peptides with alternating hydrophobic and hydrophilic residues or stretches that interact with the extracellular matrix are also disclosed in WO2008 / 113030A2. WO2010 / 103887A1 discloses self-assembling peptides containing basic, hydrophobic and acidic amino acids of a specific primary sequence, and peptide gels thereof with high strength.

[0013] Another application, WO2007 / 000979A1, describes self-assembling peptides with polar and non-polar amino acids. The peptides can form beta-sheet structures in which the non-polar amino acid residues are arranged on one side of the structure in an organized form. US Patent No. 6,548,630 describes amphiphilic self-assembling peptides for use as stable macroscopic membranes for use in biomaterial applications such as slow diffusion drug delivery.

[0014] EP 2 327 428A2 refers to a pharmaceutical composition comprising mutually complementary self-assembling peptide nanofibers and at least one cell for repairing damaged tissue, such as tissue after myocardial infarction.

[0015] The use of self-assembling peptides for the delivery of bioactive agents is described, for example, in US2008 / 199431A1 and WO2009 / 026729A1. WO2006 / 073889A2 relates to compositions in which human PDGF is directly bound to peptides that assemble into a gel that slowly releases PDGF in vivo. WO2006 / 047315A2 proposes binding therapeutic agents to self-assembling peptides using biotin / streptavidin binding.

[0016] Kirkham et al. and Kind et al. report on self-assembling peptide scaffolds that promote enamel remineralization (Kirkham et al. 2007, Dent. Res. 86(5), 426-430; Kind et al. 2017, Journal of Dental Research 1-8, doi10.1177 / 0022034517698419).

[0017] To effectively induce remineralization, for example to treat dental lesions, especially subsurface lesions (i.e. early caries lesions or white spots), the self-assembling peptide needs to be in a monomeric form outside the dental lesion, and it needs to diffuse into the lesion and switch to an organized form once inside the dental lesion. If the peptide assembles outside the lesion, it cannot promote remineralization in lesions with low pH and high ionic strength, because the three-dimensional structure formed is too large to diffuse through the pores. Therefore, for this purpose, it is necessary to prevent the peptide from assembling until it reaches its site of action.

[0018] For example, WO2014 / 027012A1 and EP2 698 162A1 provide lyophilized solutions containing monomeric self-assembling peptides for targeted treatment of dental lesions. The solution containing the monomeric peptides must be applied directly to the surface of the incipient caries lesion, limiting application to professional users, e.g. dentists. Furthermore, pretreatment of the teeth to be treated is very complex and includes professional tooth cleaning to remove plaque, food debris and stains, treatment with sodium hypochlorite and phosphoric acid, followed by rinsing and drying of the tooth surface with water. Brunton et al., 2013, Br. Dent. J. 215(4): E6, doi:10.1038 / sj.bdj.2013.741) ensure that prior to treatment the lesions are cleaned with a prophylactic paste and treated with an etching solution for 20 seconds to open the pores of the sub-surface lesions, followed by washing and drying. The lyophilized self-assembling peptide in monomeric form was rehydrated with sterile water and a drop of the resulting solution was immediately applied directly to the lesion surface. Moisture control was ensured until the P11-4 solution was no longer visible (approximately 2 min). Subjects were asked not to brush their teeth in the treated quadrants until 4 days after treatment. Schlee et al., 2014, Stomatologie 111:175-181 confirms the need for pretreatment and moisture control.

[0019] The complex procedures entail significant costs for the patient and / or associated health care systems. Additionally, for psychological reasons, some patients avoid or delay visits to a dental practitioner until the caries lesions have progressed extensively and the dentist would prefer drilling and filling over a treatment approach using self-assembling peptides.

[0020] WO2017 / 202940A1 or WO2017 / 202943A1 provide a personalized dental treatment product which can be used by a subject without the need for a dental professional to diagnose or intervene in the treatment or prevention of dental lesions and / or for use in remineralizing tooth surfaces, comprising self-assembling peptides in monomeric form, where it is emphasized that it is essential that the monomeric form is maintained for as long as possible after application to the subject's mouth.

[0021] Organized forms of self-assembling peptides (also called polymeric self-assembling peptides) can also be applied to tooth surfaces. It has been shown in a pH cycling model that the self-assembling peptide P11-4 (Curodont Protect, Credentis, Switzerland) results in increased mineralization (Soares et al., 2017. Journal of Clinical and Diagnostic Research 11(4) : ZC136-ZC141). Self-assembling peptide matrices were further found to prevent artificial caries lesions and result in remineralization of enamel around orthodontic brackets (Jablonsky-Momeni et al., 2019. Randomised in situ clinical trial investigating self-assembling pep-tide matrix P11-4 in the prevention of artificial caries lesions. Scientific Reports 9:269). A gel containing organized self-assembling peptides (Curodont D'Senz, credentis AG, Windisch, CH) also effectively blocks dentin tubules and may therefore be used for the treatment of dentin hypersensitivity (Schlee et al., Journal of Periodontology 89(6) :653-660).

[0022] EP 2 853 256 A1 and WO 2015 / 044268 A1 teach that self-assembling peptide hydrogels, i.e. dental care products comprising assembled (polymeric) self-assembling peptides and further comprising mineral particles of a particular size as well as fluorophores, which may be amino acids of the self-assembling peptides, are useful for tooth whitening, where the self-assembling peptides and the mineral particles have a synergistic effect. Summary of the Invention

[0023] In light of the state of the art, the inventors have solved the problem of providing a dental care product for use in the treatment of tooth demineralization or for use to reduce or prevent further tooth demineralization in subjects with demineralized teeth. Advantageously, the dental care product of the present invention is easy to administer, preferably for over-the-counter or retail sale, and can be applied by the patient or consumer to prevent tooth demineralization.

[0024] This problem is solved by the present invention, in particular by the claimed subject matter.

[0025] In a first aspect, the present invention provides a dental care product suitable for preventing further demineralization of the teeth of a subject having demineralized teeth, comprising: (i) a self-assembling peptide capable of undergoing self-assembly at a pH of less than 7.5, preferably comprising the sequence of SEQ ID NO: 3, wherein the self-assembling peptide is essentially present in an assembled form in the dental care product (i.e., at least 80%, preferably at least 90%, more preferably at least 95%, or most preferably at least 99%); and (ii) a pharma- ceutically acceptable base wherein the dental care product is an essentially solid product selected from the group consisting of chewing gum, soft chew, taffy, gelatin gum, chewy candy, chew toy, marshmallow, lozenge, or tablet; The dental care product is not abrasive.

[0026] The inventors have found that the administration of the dental treatment product of the present invention, preferably by chewing, results in the formation of a protective layer or film on the teeth of the subject to which it is administered.The protective layer may then prevent further demineralization and protect the teeth from acid attack.This is particularly relevant under conditions in which tooth remineralization is reduced, and therefore remineralization strategies such as the administration of monomeric self-assembling peptides are less efficient than under normal conditions (e.g., healthy subjects) or are not feasible at all.

[0027] Preferably, the dental care product is a chewy product, i.e. a product suitable for chewing or biting. In particular, preferably, when the dental care product is a lozenge or tablet, it is a chewable lozenge (e.g., according to Umashankar et al, 2016. International Research Journal of Pharmacy. 7. 9-16.) or a chewable tablet (e.g., based on the composition disclosed in Dewsbury et al. BMC Vet Res. 2019;15(1):394; Fasoulas et al, 2019. Heliyon 5(7):e02064.; Jagdale et al, 2010. Int. J Res. Pharm. Sci. 1(3), 282-289, 2010). It may be, for example, a soft product. The dental care product typically comprises one or more typical components of a respective dental care product, such as a typical pharma- ceutically acceptable base having a pH of less than 7.5, in which the self-assembling peptide may be incorporated.

[0028] The self-assembling peptides in their assembled form are preferably embedded in a pharma- ceutically acceptable base, preferably embedded in, for example, a gum base, which may contain polymers (or elastomers), plasticizers and / or resins, which are typical ingredients, for example, when the product is a chewing gum.

[0029] For example, the elastomer may be any water insoluble polymer known in the art, including the gum polymers utilized for chewing gum and bubble gum that are listed in the Food and Drug Administration, CFR, Title 21, Section 172,615 as "Masticatory Substances of Natural Vegetable Origin" and "Masticatory Substances, Synthetic."

[0030] For example, gum base is a) synthetic components selected from the group consisting of butadiene-styrene rubber, isobutylene-isoprene copolymers (butyl rubber), paraffin (produced by the Fischer-Tropsch process), petroleum-based (way), petroleum synthetic wax, low molecular weight elastomers such as polyisobutylene polyvinyl acetate, polyisobutadiene and isobutylene-isoprene copolymers, polybutene, polybutadiene and polyisobutylene, vinyl polymer elastomers such as polyvinyl acetate, polyethylene, vinyl copolymer elastomers such as vinyl acetate / vinyl laurate, vinyl acetate / vinyl stearate, ethylene / vinyl acetate, polyvinyl alcohol, or mixtures thereof; and / or b) a natural ingredient selected from the group consisting of: - Chicle, Chiquibul, Crown Gum, Guttahankan, Masalanduba Bharata, Masalanduba Chocolate, Nispero, Los Diñas, Venezuelan Chicle (all from the Sapoaceae family), - Jelutong / Lechesaspi (sorva), Pendare, Perillo (all from the Apocynaceae family), - Leche de vaca, nigergutta, tuno (all from the family Mulberry), - Tilte and natural rubber (all from the Euphorbiaceae family) may include:

[0031] Chicle is the most commonly used gum base when a natural gum base is desired, e.g., when synthetic ingredients such as mineral oil residues are to be avoided. Natural ingredients have the advantage of being biodegradable, which is particularly desirable for products that are typically expelled after chewing.

[0032] Synthetic components are commercially available and typically inexpensive.

[0033] The chewing gum of the present invention can be hard or soft.The soft chewing gum containing self-assembling peptide can be prepared according to, for example, Shivang A Chaudhary et al., Int J Pharm Investig. 2012 Jul-Sep; 2(3); 123-133; Abolfazl Aslani et al., Adv Biomed Res. 2013, 2:72; Zumbe et al., 2001, British Journal of Nutrition 85, Suppl. 1, S31-S45 (especially FIG. 8); WO2014 / 152952A1; WO2006 / 127559A2 or WO2007 / 143989A1. Hard chewing gum may include, for example, a gum base, xylitol, a self-assembling peptide such as P11-4 (eg, about 100 μg per piece of chewing gum), sodium bicarbonate, tartaric acid, and flavorings such as lemon flavor.

[0034] The chewing gum may be a compressed gum, a center-filled gum (with the assembled self-assembling peptide in the center or in a coating, preferably in the center) or a regular gum. Preferably, the assembled self-assembling peptide is embedded in the matrix of the dental care product, i.e., the gum base of the chewing gum, where uniform embedding has the advantage that the self-assembling peptide is in continuous contact with the patient's teeth during chewing over an extended period of time.

[0035] Alternatively, the dental care product of the present invention may comprise gelatin, egg white, lecithin, maltitol / sorbitol matrix, pectin or starch as a pharma- ceutically acceptable base for, for example, a soft chew, gelatin gum, marshmallow or chew toy. For example, a marshmallow of the present invention typically comprises egg white and / or gelatin, a sweetener, water, a self-assembling peptide and air. A soft chew of the present invention typically comprises a maltitol / sorbitol matrix and a self-assembling peptide.

[0036] For example, the pharma- ceutically acceptable base for the gelatin gum of the present invention may comprise, for example, gelatin tin and / or pectin, water, sugar or sugar substitute, for example in the form of syrup, tartaric acid and / or citric acid. The gelatin gum of the present invention comprises assembled self-assembling peptides, where typically the self-assembling peptides are added as assembled premixes. The gelatin gum of the present invention may be coated with self-assembling peptides. Exemplary sugar-free gelatin gums and their preparations are described in Zumbe et al., 2001, British Journal of Nutrition 85, Suppl. 1, S31-S45, especially in Table 13 or Table 14, where the self-assembling peptides are added in a polymeric state, or the self-assembling peptides are added in any form, for example partially or completely monomeric, and the pH in the mixture with the base is less than 7.5, which results in self-assembly. If the dental care product comprises gelatin, it may be type A or type B gelatin. The pH cannot be lowered to a level that would prevent the gelatin from gelling.

[0037] The subject may be a human subject, but may also be an animal subject, for example a pet, such as a dog, horse, or cat. Chew toys (or chew animal biscuits) may be a particularly suitable form of dental care product suitable for use with animals, for example pets, such as cats, dogs, or horses, especially dogs. Chew toys may be coated with and / or preferably incorporate assembled self-assembling peptides. Such embodiments provide low-cost and labor-intensive access to treatment and preventive measures for demineralization of animal teeth. Chew toys of the present invention may include gelatin or pectin and self-assembling peptides, together with flavors that are typically enjoyed by animals.

[0038] The toffee comprises an emulsion of fat in an aqueous system. The self-assembling peptide toffee of the present invention typically comprises, in addition to the self-assembling peptide, a sugar or sugar substitute, for example maltitol, sorbitol, xylitol, lactitol and / or isomalt, optionally at least partially in the form of a syrup, such as maltitol syrup, a vegetable oil, for example coconut oil, soybean oil, sunflower oil, rapeseed oil, olive oil and / or peanut oil, a gelling agent, such as gelatin and / or pectin, and optionally vitamins, antioxidants, for example citric acid and / or ascorbic acid, flavourings, colourings, sweeteners, for example acesulfame K, aspartame and / or sucralose. Exemplary sugar-free taffies and their preparation are taught in Zumbe et al., 2001, British Journal of Nutrition 85, Suppl. 1, S31-S45, especially Table 12, where self-assembling peptides are added and the pH is adapted to ensure their assembled state, or the self-assembling peptides are added in an assembled state.

[0039] The peptide chewable lozenges or chewable tablets of the present invention may further comprise, in addition to the self-assembling peptides in an organized state, ingredients such as sugars or sugar substitutes, e.g., maltitol, sorbitol, xylitol, lactitol and / or isomalt, and optionally antioxidants, e.g., citric acid and / or ascorbic acid, flavors, colorants and sweeteners, e.g., aspartame, acesulfame K, sucralose.

[0040] The peptide concentration in the dental care product of the invention may be 0.1-5000mg peptide / kg bulk product, such as 0.1-1000mg peptide / kg bulk product, 0.1-500mg peptide / kg bulk product, preferably 0.1-100mg peptide / kg bulk product, 0.5-50mg peptide / kg bulk product, 1-20mg peptide / kg bulk product, or most preferably 5-15mg peptide / kg bulk product or 8-10mg peptide / kg bulk product. The following examples show that such concentrations allow the reduction or prevention of tooth demineralization.

[0041] The preferred peptides of the present invention, the self-assembling peptides, are provided, for example, in WO2004 / 007532A1, which is fully incorporated herein by reference, and discloses peptides that can form three-dimensional scaffolds and thereby promote the nucleation of de novo calcium phosphate. These artificial peptides assemble in one dimension to form beta sheets and form higher-order assemblies, such as tape-like assemblies. Three-dimensional supramolecular structures of self-assembling proteins can be formed, which have an affinity for calcium phosphate.

[0042] Some other self-assembling peptides (SAPs) that may be used are described in the prior art. For example, WO2010 / 041636A1 describes a bioadsorbable peptide tissue occluding agent that includes an artificial peptide having 8-200 amino acid residues with alternating hydrophilic and hydrophobic amino acids and self-assembles into a β-structure at physiological pH. Self-assembling peptides with alternating hydrophobic and hydrophilic residues or stretches that interact with the extracellular matrix are also disclosed in WO2008 / 113030A2. WO2010 / 103887A1 discloses self-assembling peptides that include specific primary sequences of basic, hydrophobic and acidic amino acids, and peptide gels thereof with high strength. WO2010 / 019651A1 relates to other self-assembling peptides.

[0043] Another application, WO2007 / 000979A1, describes self-assembling peptides with polar and non-polar amino acids. In the assembled form, the peptides can form beta-sheet structures in which non-polar amino acid residues are arranged on one side of the structure. In US6,548,630, amphiphilic self-assembling peptides are described for use as stable macroscopic membranes for use in biomaterial applications such as slow diffusion drug delivery.

[0044] EP 2 327 428A2 refers to a pharmaceutical composition comprising mutually complementary self-assembling peptide nanofibers and at least one cell for repairing damaged tissue, such as tissue after myocardial infarction.

[0045] In the context of the present invention, the self-assembling peptide taught in WO2004 / 007532A1 is particularly preferred. Most preferably, said self-assembling peptide is the self-assembling peptide designated as oligopeptide 104 or P11-4 (SEQ ID NO: 1, QQRFEWEFEQQ), or the self-assembling peptide with SEQ ID NO: 3, QQRFOWOFEQQ (also referred to as P11-8), or the self-assembling peptide with SEQ ID NO: 20, QQRQEQEQEQQ (also referred to as P11-20), or it comprises any of said peptides. The self-assembling peptide comprising or consisting of SEQ ID NO: 1 is most preferred throughout the present invention. It may also be a self-assembling peptide having at least 60% sequence identity to the peptide consisting of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13. Preferably, the peptide has at least 70%, at least 80%, or at least 90% sequence identity to a peptide consisting of SEQ ID NO:1, preferably SEQ ID NO:3 or SEQ ID NO:20. Most preferably, the peptide has or is at least 60%, at least 70%, at least 80%, or at least 90% sequence identity to a peptide consisting of SEQ ID NO:1. Alternatively, the peptide may have or be at least 60%, at least 70%, at least 80%, or at least 90% sequence identity to a peptide consisting of SEQ ID NO:3. Alternatively, the peptide may have or be at least 60%, at least 70%, at least 80%, or at least 90% sequence identity to a peptide consisting of SEQ ID NO:20. Examples of self-assembling peptides that may be used in the present invention are shown in Table 1 below.

[0046] The self-assembling peptides may be modified peptides containing an Ac-N-terminus and / or an NH2-C-terminus, or may be unmodified peptides. [Table 1]

[0047] To allow the mineral particles to be bound to the tooth surface, the matrix must be able to bind and adhere the mineral particles to the tooth surface. Thus, the matrix preferably includes binding sites for the mineral particles that allow the calcium-containing particles to be bound to the tooth surface. For example, charged amino acid residues such as Glu or Orn on the surface of the self-assembling peptides bind with hydroxyapatite particles and bind to the tooth surface, which is essentially hydroxyapatite. Without intending to be bound by theory, it is believed that both reactions increase the stability of the complex formed and create a more permanent whitening effect. The ability of three-dimensional self-assembly, for example found in collagen, supramolecular assemblies, or self-assembling peptides, is important for the binding. In general, a highly charged surface promotes the attachment of the mineral particles. Protein matrices are particularly effective when glutamic acid or ornithine residues are found on the surface, which may bind to calcium phosphate or other mineral particles. Preferably, the protein includes 5% or more, 10% or more, 20% or more, or 30% or more of charged amino acid residues such as glutamic acid and / or ornithine residues.

[0048] The preferred self-assembling peptides of the present invention have common features that can be summarized in a consensus sequence.In particular, the self-assembling peptides used in the product of the present invention comprise the sequence of the formula X1-X2-X1-X2-X1, where X1 is an amino acid with acidic or basic side chain, and X2 is an amino acid with hydrophobic or polar side chain selected from the group consisting of alanine, valine, isoleucine, leucine, methionine, phenylalanine, tyrosine, tryptophan and glutamine (SEQ ID NO: 21).X2 can also be an amino acid with neutral side chain.

[0049] In a first embodiment, X1 is an amino acid with a basic side chain. When X1 is an amino acid with a basic side chain, the amino acid is preferably ornithine or arginine. Of course, X1 may be different basic amino acids at different positions of the same self-assembling peptide. Preferably, X1 at position 1 of SEQ ID NO:21 is Arg, and X1 at positions 3 and 5 of SEQ ID NO:21 is Orn. ​​Amino acid side chains with basic side chains are protonated at pH values ​​below their nominal pK value. Self-assembling peptides where X1 is an amino acid with a basic side chain assemble at high pH, ​​for example, pH above 7.5. P11-8, peptide of SEQ ID NO:3, is a preferred example of such a self-assembling peptide.

[0050] In the second embodiment preferred herein, X1 is an amino acid having an acid side chain, i.e. a side chain containing a -COOH group.The self-assembling peptide used in these products of the present invention comprises a sequence of the formula X1-X2-X1-X2-X1, where X1 is an amino acid having an acidic side chain, and X2 is an amino acid having a hydrophobic or polar side chain selected from the group consisting of alanine, valine, isoleucine, leucine, methionine, phenylalanine, tyrosine, tryptophan, and glutamine (SEQ ID NO: 22).X2 may also be an amino acid having a neutral side chain.

[0051] Amino acid side chains with -COOH are deprotonated at pH values ​​above their nominal pK value. For example, amino acids containing -COOH groups in their side chains, such as aspartic acid (Asp, D) and glutamic acid (Glu, E), are essentially deprotonated at neutral, i.e., pH above 7, because they exhibit low pKa (Asp: 3.71; Glu: 4.15). In the self-assembling peptides used in the products of the present invention, the amino acid side chains containing -COOH groups are specifically positioned within the peptide chain to control the electrostatic interactions between adjacent peptides. When the -COOH groups are deprotonated to -COO-, identical self-assembling peptides are repelled by electrostatic interactions, governing the association free energy in the bond between peptides. When the pH is lowered below a certain threshold, i.e., the pH at which peptides begin to self-assemble, such as about pH 7.5 for P11-4 (SEQ ID NO: 1), the self-assembling peptides of the present invention reduce the repulsive electrostatic interactions between peptides, allowing them to self-assemble.

[0052] Preferred examples of self-assembling peptides of the invention capable of self-assembly at a pH below 7.5 (especially having SEQ ID NO:22) are P11-4 (SEQ ID NO:1) and P11-20 (SEQ ID NO:20).

[0053] For example, X2 may be an amino acid with a polar neutral side chain, such as glutamine. Thus, the self-assembling peptide used in the product of the invention may comprise the sequence Glu-Gln-Glu-Gln-Glu (SEQ ID NO: 26). In this case, the preferred self-assembling peptide is P11-20.

[0054] The peptides used in the products of the invention may also comprise a sequence of the formula X1-X2-X1-X2-X1, where X1 is an amino acid having an acidic side chain and X2 is an amino acid having a hydrophobic side chain selected from the group consisting of alanine, valine, isoleucine, leucine, methionine, phenylalanine, tyrosine, and tryptophan (SEQ ID NO:23).

[0055] In a preferred embodiment, the self-assembling peptide used in the products of the invention comprises the sequence Glu-X2-Glu-X2-Glu, where X2 is an amino acid having a hydrophobic side chain selected from the group consisting of alanine, valine, isoleucine, leucine, methionine, phenylalanine, tyrosine, and tryptophan (SEQ ID NO:24) or the sequence Asp-X2-Asp-X2-Asp, where X2 is an amino acid having a hydrophobic side chain selected from the group consisting of alanine, valine, isoleucine, leucine, methionine, phenylalanine, tyrosine, tryptophan (SEQ ID NO:25).

[0056] Complementary self-assembling peptides may also be used in the context of the present invention. Examples of complementary peptides are provided, for example, in EP 2 327 428 A2.

[0057] Preferably, the self-assembling peptide used in the product of the invention comprises or consists of the sequence Gln-Gln-Arg-Phe-Glu-Trp-Glu-Phe-Glu-Gln-Gln (P11-4, SEQ ID NO:1), or a sequence having at least 80%, preferably 90%, sequence identity thereto. It is further preferred that the peptide is a modified P11-4, in particular acylated at position 1 and amidated at position 11, or a sequence having at least 80%, preferably 90%, sequence identity thereto. SEQ ID NO:1 is a preferred variant of SEQ ID NO:23 and SEQ ID NO:24.

[0058] For the peptide referred to herein as P11-4, the switch from monomeric to organized multimeric form is controlled by pH: when the pH is below pH 7.5, the peptide assembles; when the pH is higher, the peptide state is monomeric.

[0059] Peptides with at least 80% or more sequence identity to SEQ ID NO:1 preferably contain glutamic acid or aspartic acid at positions corresponding to amino acids 5, 7 and 9 of SEQ ID NO:1. Specifically, peptides with at least 80% or more sequence identity to SEQ ID NO:1 preferably contain glutamic acid at positions corresponding to amino acids 5, 7 and 9 of SEQ ID NO:1. Preferably, the remaining amino acid positions are amino acids with hydrophobic side chains selected from the group consisting of alanine, valine, isoleucine, leucine, methionine, phenylalanine, tyrosine and tryptophan. Preferably, the remaining amino acid positions are not amino acids with basic side chains, i.e., amino acids that are positively charged at near-neutral pH.

[0060] In one embodiment, the peptides used in the products of the invention comprise or consist of sequences different from those shown in SEQ ID NO: 1, 3, or 20, preferably by substitution of 1, 2, or 3 amino acids. In general, each of the amino acid residues in the peptide sequence of SEQ ID NO: 1, 3, or 20 may be replaced by another residue, so long as the resulting peptide can undergo self-assembly at a pH value lower than 7.5. The substitution is preferably a conservative substitution, i.e., the replacement of one or more amino acid residues with amino acids of similar polarity that act as functional equivalents. Preferably, the amino acid residues used as substitutions are selected from the same amino acid group as the amino acid residues to be replaced. For example, a hydrophobic residue can be replaced with another hydrophobic residue, or a polar residue can be replaced with another polar residue with the same charge. Functionally homologous amino acids that may be used for conservative substitutions include, for example, non-polar amino acids such as glycine, valine, alanine, isoleucine, leucine, methionine, proline, phenylalanine, and tryptophan. Examples of uncharged polar amino acids include serine, threonine, glutamine, asparagine, tyrosine, and cysteine. Examples of charged polar (basic) amino acids include histidine, arginine, and lysine. Examples of charged polar (acidic) amino acids include aspartic acid and glutamic acid.

[0061] Furthermore, the peptides used in the products of the invention may be structurally modified at one or more amino acid positions, for example by the introduction of one or more modified amino acids. According to the invention, these modified amino acids may be amino acids that have been altered, for example, by biotinylation, phosphorylation, glycosylation, acetylation, branching and / or cyclization. Furthermore, the peptides of the invention may additionally or alternatively contain other modifications, such as terminal blocking groups, formyl groups, gamma-carboxyglutamic acid hydroxyl groups, methyl groups, phosphoryl groups, pyrrolidone carboxylic acid groups, and / or sulfate groups. In a preferred embodiment, all peptides of the invention are acetylated at their N-terminus and / or amidated at their C-terminus, for example at the NH2 group, most preferably both. A particularly preferred embodiment is peptide P11-4, which is acetylated at the N-terminus and amidated at the C-terminus at the NH2 group.

[0062] The size of the self-assembling peptide used in the product of the present invention is not particularly limited. The peptide of the present invention can be any length that allows self-assembly in a pH-dependent manner. Preferably, the peptide has a size of about 5-200 amino acids, more preferably 9-100 amino acids, 10-50 amino acids, 10-30 amino acids or 11-20 amino acids. Even more preferably, the self-assembling peptide has a length of about 27 amino acids, 24 amino acids, 21 amino acids, 15 amino acids or 11 amino acids. In a particularly preferred embodiment, the self-assembling peptide has a length of 11 amino acids.

[0063] The self-assembling peptide may be prepared by any suitable method generally known in the field of peptide synthesis. For example, peptides having a length of more than 50 amino acids may be prepared by recombinant methods. In one embodiment, the self-assembling peptide is generated as a fusion peptide. As used herein, a fusion peptide refers to a fusion of a first amino acid sequence comprising a self-assembling peptide of interest linked at its N-terminus or C-terminus to a second amino acid sequence. The second amino acid sequence may be an affinity tag, i.e., an amino acid sequence fused to the N-terminus or C-terminus of the self-assembling peptide that exhibits increased affinity for another compound, thereby allowing purification of the fusion peptide. Preferably, the tag sequence is removed from the self-assembling peptide of interest after purification, for example by providing a proteolytic cleavage site between the self-assembling peptide and the affinity tag. In one embodiment, the self-assembling peptides are prepared as disclosed in Kyle et al., 2010, Biomaterials 31, 9395-9405 and Kyle et al. 2009, Trends in Biotechnol. 27 (7), 423-433.

[0064] Smaller self-assembling peptides are usually prepared by chemical synthesis. For example, peptides may be chemically synthesized by solid-phase or solution-phase methods. Protocols for solution-phase chemical synthesis of peptides have been described (see, for example, Andersson et al., Biopolymers 55:227-250, 2000). For solid-phase synthesis, the technique described by Merrifield (J. Am. Chem. Soc., 1964, 85, 2149-2154) may be used. In this approach, the growing peptide is immobilized on an insoluble resin and unreacted soluble reagents are removed by filtration or washing steps without any handling losses. Solid-phase peptide synthesis can be easily performed using automated equipment.

[0065] The peptides used in the products of the present invention may contain any naturally occurring proteinogenic amino acid. In addition, the peptides may also contain unusual non-proteinogenic amino acids such as carnitine, gamma aminobutyric acid (GABA), hydroxyproline, selenomethionine, hypusine, lanthionine, 2-aminoisobutyric acid, dehydroalanine, ornithine (Orn, O), citrulline, beta-alanine (3-aminopropanoic acid), etc. Non-proteinogenic amino acids can be incorporated into the peptides by post-translational modification or by direct incorporation during the chemical synthesis of the peptide.

[0066] The self-assembling peptides used in the product of the present invention undergo self-assembly in response to a certain pH and ionic strength.In one embodiment, the preferred self-assembling peptides for use according to the present invention are selected to undergo self-assembly as soon as the pH of their environment falls below a certain pH, for example below pH 7.5.The pH at which the self-assembling peptides of the present invention begin to self-assemble is less than 7.5, preferably less than 7.2, more preferably less than 7.0.For example, the pH at which the self-assembling peptide P11-4 (SEQ ID NO: 1) and the end-modified P11-4 begin to self-assemble is about 7.5.This means that when the pH falls below 7.5, the self-assembling peptides begin to self-assemble to a large extent.

[0067] As used herein, the pH at which self-assembling peptides begin to self-assemble refers to the pH below the pH at which peptides are observed to self-assemble to a significant degree in solution, which means that at least about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 99% or even about 100% of the peptides found in dental care products are assembled.In a preferred embodiment, at least about 25% of the peptides found in dental care products are assembled below the pH at which peptides begin to self-assemble.

[0068] Preferably, the pH is at which self-assembly begins, for example, at 100-100° C. For P11-4 and modified P11-4, at about pH 7.5, about 20% or less of the peptides, preferably about 15% or less, more preferably 10% or less, and even more preferably 5% or less, are in a multimeric state.

[0069] In contrast, below the pH at which self-assembly begins, below pH 7.5 for P11-4 (SEQ ID NO:1), a significant degree of self-assembly of the peptides in solution is observed, meaning that at least about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 99% or even about 100% of the peptides found in solution are organized, i.e., multimeric or macromolecular.

[0070] Preferably, the ionic strength at which the peptides self-assemble is physiological ionic strength.

[0071] As used herein, "self-assembly" of a peptide refers to the spontaneous and reversible organization of a peptide and other peptides of its own type (or peptides with similar structure) into multimeric assemblies through non-covalent interactions. Non-covalent interactions involved in the formation of multimeric assemblies include van der Waals, pi-stacking, hydrogen bonding, polar and ionic interactions between the amino acid backbone and / or amino acid side chains of the peptides.

[0072] The self-assembling peptides used in the products of the invention are preferably organized into beta-pleated sheets. In beta-pleated sheets, a series of hydrogen bonds between residues of different polypeptide chains or between residues of different sections of a folded polypeptide create a sheet-like structure. Typically, adjacent polypeptide chains in a beta-pleated sheet are antiparallel, meaning that they run in opposite directions. However, adjacent chains may run parallel. If several polypeptide chains are involved in sheet formation, the sheet is a rigid wall-like structure. Multiple pleated sheets provide the necessary toughness and rigidity. The peptides that can be used in the products of the invention form stable secondary structures upon self-assembly. Preferably, the peptides used in the invention form long "beta tapes" that include beta-pleated structures of a single molecule thickness. The peptides may form complex structures during assembly, such as helical tapes (single molecule thickness), twisted ribbons (double tapes), fibrils (twisted stacks of ribbons), and fibers (entangled fibrils). When the pH is lowered, helical tapes, twisted ribbons, fibrils, and finally fibers may form. Preferably, the dental care products of the present invention comprise fibres of self-assembling peptides.

[0073] As known to those skilled in the art, the organized state of peptides is also influenced by ionic strength. The ionic strength of a solution is a function of the concentration of all ions present in the solution. Thus, a particularly high ionic strength can induce peptides to assemble at a pH higher than the pH at which peptides begin to self-assemble, i.e., even when the peptides are substantially monomeric in solution.

[0074] At ionic strengths in the physiological range, i.e., corresponding to 150 mM NaCl, P11-4 assembles at neutral pH (Carrick et al., 2007. Tetrahedron 63(31):7457-7467). Those skilled in the art know how to determine and measure the ionic strength of a solution. Ionic strength, I, is generally determined by the formula I=1 / 2Σzi 2 b i where z is the valence coefficient, bi is the molar concentration of the i-th ion [mol / kg{H 2 O}]. The sum Σ applies to all ions in the solution. For example, a 150 mM NaCl solution has an ionic strength of about 0.15. This is also roughly the same as the ionic strength of blood. The ionic strength of saliva, present in the oral cavity, is typically much lower, for example around 0.04.

[0075] Those skilled in the art are aware of numerous methods for determining the ionic strength of a preparation. For example, ionic strength may be estimated from a measurement of the solution's conductivity (S=1 / Ω=A / V) via the Russell coefficient as follows: I=1.6x10 -5 x Specific conductivity [μS / cm]. The conductance of a 150 mM NaCl solution is about 80-100 mS / cm. Thus, according to the conductivity estimates above and described, the dental care product has a conductivity of less than 100 mS / cm, preferably less than 80 mS / cm.

[0076] Moreover, those skilled in the art are aware of numerous methods for determining the pH at which the peptides of the invention begin to self-assemble at a given ionic strength. Suitable methods are given, for example, in the publication by Aggeli et al. (2003, J Am Chem Soc, 125, 9619-9628).

[0077] Those skilled in the art can determine whether essentially all of the self-assembling peptides are in an assembled form by routine experimentation. For example, the assembled state of the peptides in solution can be determined by nuclear magnetic resonance (NMR), such as 1H-NMR, circular dichroism analysis, dynamic light scattering (DLS) analysis, diffusing wave spectroscopy, native electrophoresis, viscosity measurement (rheology), quartz crystal microbalance with dissipation monitoring (QCMD), preferably by intrinsic electrophoresis. The presence of fibers of the self-assembling peptides can be detected by TEM, as described in the following examples.

[0078] It is known to those skilled in the art that peptide concentration may affect peptide assembly, i.e. particularly high peptide concentrations may induce assembly. Furthermore, exceptionally low peptide concentrations, i.e. even under low pH conditions such as those present in dental lesions and in the oral cavity, may prevent assembly of the peptides of the invention.

[0079] Typically, the pH of the dental care product of the present invention is within the range in which the dental care product of the present invention is assembled. Thus, for a self-assembling peptide comprising SEQ ID NO: 22, the pH is preferably less than 7.5, or for a self-assembling peptide of SEQ ID NO: 21, X1 is an amino acid with a basic side chain, the pH is greater than 7.5. However, for a self-assembling peptide comprising SEQ ID NO: 22, such as P11-4, it is also possible to change the pH, for example, to a pH of 8.5, or to a pH of 11 (Carrick et al., 2007), or even to a pH of 5 for a self-assembling peptide of SEQ ID NO: 21, where X1 is an amino acid with a basic side chain, while maintaining the self-assembling peptide in an assembled form. For example, a high ionic strength and / or concentration of the self-assembling peptide may lead to the maintenance of the assembled form.

[0080] Thus, for example, it is possible to prepare a dental treatment product of the present invention with a slightly basic pH, for example, pH 7.5-8 or 8-8.5, containing P11-4 or P11-20 (preferably P11-4) in an assembled form. In this case, a premix of assembled peptides, preferably containing mainly fibers of self-assembling peptides, is mixed with a matrix buffered at a desired pH, where the ionic strength and / or concentration of the self-assembling peptides maintains the self-assembling peptides in an assembled form. For example, when administered after a meal or acidic drink, the basic pH of the dental treatment product may neutralize the pH of the mouth. In this environment, the assembled self-assembling peptides may then form a protective layer on the teeth.

[0081] For dental care products of the invention comprising SEQ ID NO: 21, where X1 is an amino acid with a basic side chain, it may be advantageous for the organized morphology to be maintained at a slightly acidic pH, for example in the range of 7-7.5, 6-7 or even 5-6, e.g. P11-8, since acidic pH is typically considered more palatable to humans.

[0082] Suitable buffers and pH adjusting agents to obtain a desired pH are known in the art.

[0083] In order to prevent the abrasive action on already demineralized teeth, the dental care product of the present invention is not abrasive. Wear is usually caused by particles. In particular, the dental care product of the present invention does not contain or is essentially free of abrasives at a suitable concentration, and is essentially free of particles, especially mineral particles. Commonly used abrasives are calcium carbonate, silica, aluminum hydroxide, and phosphates of aluminum or calcium. If the dental care product of the present invention is abrasive (like typical toothpaste), it will lead to further abrasion or erosion of already demineralized teeth, since it is intended for chewing.

[0084] Abrasion depends on different parameters, especially hardness, size and shape of the particles. Preferably, the dental treatment product does not contain a significant amount of hard particles, especially particles with a MOSH hardness higher than the MOSH hardness of dentin, which is 3. Large particles are more abrasive than small particles. Therefore, it is preferred that the dental treatment product does not contain a significant amount of large particles, for example with a size of 1 μm or more, 0.5 μm or more, or 0.1 μm or more. Round particles are less abrasive than particles with edges. Therefore, the amount of round particles that may be contained is greater than the amount of particles with edges that are otherwise comparable.

[0085] Preferably, the dental care products of the invention do not contain more than 0.4% by weight of mineral particles having a size of at least 0.1 μm, more preferably they contain less than 0.3% by weight of mineral particles having a size of at least 0.1 μm, or less than 0.2% by weight of mineral particles having a size of at least 0.1 μm, or less than 0.1% by weight of mineral particles having a size of at least 0.1 μm, or less than 0.01% by weight of mineral particles having a size of at least 0.1 μm. They may also be free of mineral particles.

[0086] In order to improve the taste and acceptability of the product, the dental care product of the present invention may preferably contain sugar and / or sugar substitutes that do not promote cariogenic tooth decay, such as polyols or sugar alcohols, such as sorbitol, mannitol, maltitol, lactitol, isomalt, xylitol and / or erythritol, or D-tagatose and / or trehalose. Advantageously, the dental care product of any of the previous embodiments does not contain cariogenic sugars, such as sucrose. Thus, preferably, the products are sugar-free products, i.e., they do not contain significant amounts or any amount of sucrose or glucose. Sugar-free products may be used, for example based on suitable modifications of the preparations disclosed by Zumbe et al., 2001, British Journal of Nutrition 85, Suppl. 1, S31-S45, further comprising self-assembling peptides, and pH controlled to ensure the assembly state of the peptides, for example by selecting appropriate buffers and pH, as disclosed herein.

[0087] It is particularly advantageous if the dental treatment product contains, in addition to the self-assembling peptide, a polyol that has been shown to be anti-cariogenic, such as xylitol, erythritol, or sorbitol, preferably xylitol.It also reduces biofilm and plaque, thus facilitating the self-assembling peptide of the present invention to access the tooth surface and potential lesions.Of course, xylitol is not used for application to dogs, cows, goats, rabbits, or other animals, where the substance is toxic.Xylitol can be used in the product of the present invention for use in human subjects or, for example, cats, preferably human subjects.

[0088] The dental care products of the present invention may further comprise flavours, for example lemon flavour, caramel, vanillin, menthol, preservatives, such as ethanol, sodium benzoate, colouring agents, such as Solvent Red, Acid Blue 3, active agents such as fluorides, preferably in the form of tertiary amines, such as amine fluorides, or organic fluorides, such as sodium monofluorophosphate, potassium nitrate, and / or oxalates.

[0089] The dental care product of the invention may contain phosphates, such as sodium phosphate, calcium phosphate, e.g. hydroxyapatite, as defined above, so long as they are not abrasive. Phosphates aid in remineralization and also have a cleaning effect. The phosphates may, for example, be present in solution.

[0090] Dental care products of the present invention may also contain pyrophosphates, which have a hydrophilic effect on tooth surfaces and may prevent extrinsic staining.

[0091] Dental care products of the present invention may also contain pH adjusting agents such as sodium carbonate / bicarbonate or urea.

[0092] The buffering agent that may be used in the context of the present invention may be, for example, one or more of alkali metal carbonate, alkali metal bicarbonate, alkaline earth metal carbonate, alkali metal citrate or alkali metal phosphate, or any mixture thereof.Preferred buffering agent is sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, calcium carbonate, potassium citrate or dipotassium phosphate, or any mixture thereof.More preferably, buffering agent is sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, potassium citrate and dipotassium phosphate, or any mixture thereof.In particular, it is sodium carbonate, sodium bicarbonate, potassium carbonate or potassium bicarbonate, or any mixture thereof (for example, as disclosed in US9,511,021).

[0093] It is noteworthy that even when the dental care product has a basic pH, i.e., a pH above pH 7.5, for example, a pH between pH 7.5 and 8, if the ionic strength is high enough, the preformulated assembled self-assembling peptides will maintain their assembled form. Thus, the slightly basic dental care product of the present invention may be used to neutralize the acidic pH after eating or drinking, and may simultaneously deliver the assembled self-assembling peptides. However, in the context of human consumption, an acidic pH often results in a more pleasant taste.

[0094] In another aspect, the present invention relates to a method for preparing a dental care product of any of embodiments 1-13, comprising the steps of: a) providing a first matrix of organized self-assembling peptides; b) providing a pharma- ceutically acceptable base as a second matrix, where steps a) and b) can be performed in any order; c) mixing the first and second matrices together, optionally with other ingredients; d) forming dental care products; and e) Packaging dental care products, if desired. The present invention provides a method comprising:

[0095] The first matrix of assembled self-assembling peptides of step a) may be prepared, for example, by drying a solution having a pH below 7.5. The matrix may be a gel or a dry material obtained, for example, by spray drying / freeze drying or evaporation.

[0096] Preferably, for step a), the peptides are first monomerized by raising the pH to at least 8. The pH is then titrated, preferably slowly, to below pH 7.5 to allow for the ordered formation of self-assembling peptide beta-sheets, fibrils, and fibers. Advantageously, the pH is lowered until essentially all of the self-assembling peptides are present in an organized (or polymeric) form.

[0097] Alternatively, after generation of the self-assembling peptides, the pH can be controlled and, if necessary, reduced to a pH below 7.5 to allow the formation of self-assembling peptide beta-sheets, fibrils, and fibers if not already present. Monomerization and subsequent titration to a low pH (<7.5) is preferred because it results in a more ordered structure of the self-assembling peptides and avoids random coil structures. This results in a more uniform and reproducible product.

[0098] The pharma- ceutically acceptable base, i.e. the second matrix, may be any of the bases described herein. Preferably, it is a gum base as defined above. The preparation of such gum bases is known in the art.

[0099] In step c), the first and second matrices are mixed.Preferably, they are homogenized.Homogenization results in a substantially homogenous distribution of the self-assembling peptide in the pharma-ceutically acceptable base of the dental care product.Homogenization may be carried out by mixing, for example, using a blender, homogenizer, pass-through homogenizer, disperser, or rotating membrane, preferably using a blender.

[0100] In any case, the self-assembling peptide is preferably already in an assembled form before being mixed with a pharma-ceutically acceptable base.The inventors have found that this promotes the self-assembly of a matrix of self-assembling peptides, in particular a matrix of fibrils and fibers of self-assembling peptides.Surprisingly, homogenization does not result in the collapse of such matrix structure.Therefore, the dental care product preferably comprises assembled fibrils and / or fibers of self-assembling peptides, such as fibrils of P11-4.

[0101] The dental care product may then be formed by forming the mixture in a mold, e.g., by extrusion, by injecting or applying pressure, and / or by cutting, tearing, or forming the matrix of assembled self-assembling peptides and the hardened mixture of self-assembling peptides. For example, chewing gum is typically cut into strips, but may also be formed into balls.

[0102] In one embodiment, a matrix of assembled self-assembling peptides, prepared, for example, as in step a, can be applied in a coating step to a dental care product, such as, for example, a chewing gum, in particular, a chewing gum of the present invention. For example, a solution, preferably having a pH of less than 7.5, containing assembled self-assembling peptides, such as P11-4, can be applied as a coating, optionally by spray drying.

[0103] The dental care products can then be packaged.The packaging can be individual packaging for each product, for example in paper or foil or combinations thereof, as is customary for chewing gum, and / or bulk product packaging, for example several individual dental care products in one package, for example in glass, paper packaging, or plastic bag.Of course, the multiple packages can then be further packaged, for example for retail sale.

[0104] The packaged dental care product may include printed information, such as a leaflet or label, regarding the medical and / or cosmetic use of the product, as disclosed herein.

[0105] The present invention also provides a dental care product obtainable by the method of the present invention.

[0106] In one aspect, the present invention provides a dental care product for use in reducing demineralization of tooth surfaces in a subject having demineralized teeth, in particular for reducing further demineralization of tooth surfaces in a subject having demineralized teeth, as described herein.

[0107] In the context of the present invention, demineralized teeth does not necessarily mean the presence of caries, but rather a change in the balance of demineralization and remineralization towards tooth demineralization. For example, the subject may have a disease or disorder that promotes demineralization, such as a disease or disorder that prevents or reduces tooth remineralization that occurs in healthy subjects. The inventors have surprisingly found that application of the dental care product of the present invention may help prevent or reduce further demineralization, when remineralization often does not lead to satisfactory results. For example, the dental care product may be for use in treating subjects with xerostomia, hyposalivation, bruxism, dentin hypersensitivity, and / or tooth erosion, i.e., the subject with demineralized teeth may be a subject with one or more of these diseases or disorders. Preferably, in these diseases, administration of the dental care product of the present invention forms a layer of self-assembling peptides on the surface of the tooth, which may be eroded or demineralized, i.e., before the tooth itself is damaged, serves as a sacrificial layer.

[0108] In one embodiment, the subject has xerostomia.Xerostomia is the subjective sensation of dry mouth, and is often (but not always) associated with the hypofunction of salivary glands.Xerostomia may be associated with changes in the composition of saliva, or with a decrease in saliva flow, or may not have a specific cause.

[0109] This condition is very common and is often seen as a side effect of many types of medications. It is common in the elderly (mainly because this group tends to take several medications), or in people who abuse drugs, for example chronic users of methamphetamine, or in people who breathe through their mouths. Dehydration, radiation therapy, chemotherapy, and some diseases involving the salivary glands can cause reduced saliva production (hyposalivation), or changes in the consistency of saliva, thereby causing a complaint of xerostomia. Sometimes there is no identifiable cause, and sometimes there may be a psychogenic reason for the complaint.

[0110] Although hyposalivation is a clinical diagnosis made on the basis of history and examination, reduced saliva flow has been given an objective definition. Salivary gland hypofunction is defined as an objectively demonstrable decrease in total and / or individual gland flow rates. The unstimulated total salivary flow rate in normal individuals is 0.3-0.4 ml per minute, with anything less than 0.1 ml per minute being grossly abnormal. Stimulated salivary flow rates are reduced to less than 0.5 ml per gland in 5 minutes or less than 1 ml per gland in 10 minutes. The term subjective xerostomia is sometimes used to describe the condition in the absence of clinical evidence of dryness. Xerostomia may occur as a result of a change in the composition of saliva (from serous to mucous). Salivary gland hypofunction is an umbrella term that describes the presence of xerostomia, salivary gland hyposalivation, and hypersalivation (https: / / en.wikipedia.org / wiki / Xerostomia).

[0111] Thus, in one embodiment, xerostomia is associated with hyposalivation, e.g., an unstimulated total saliva flow rate of less than 0.1 ml per minute. A subject may also have hyposalivation without a subjective sensation of dry mouth.

[0112] Reduced saliva secretion significantly reduces the rate of tooth remineralization, thus shifting the balance towards demineralization. Without the buffering effect of saliva, cariogenic tooth decay becomes a common feature and may progress much more aggressively than it would otherwise ("endemic caries"). It may affect tooth surfaces that are normally spared, such as cervical and root caries. This is commonly seen in patients undergoing radiation therapy involving the major salivary glands, referred to as radiation-induced caries.

[0113] Decreased saliva production may be due to physiological effects such as anxiety or dehydration, or due to xerogenic drugs such as anticholinergics, sympathomimetics, and diuretics. Smoking is another possible cause. Administration of other recreational drugs such as methamphetamine, cannabis, hallucinogens, or heroin may also lead to xerostomia and dental demineralization. Xerostomia may also be caused by autoimmune conditions that damage saliva-producing cells, such as Sjogren's syndrome, e.g., primary or secondary Sjogren's syndrome. Xerostomia may also be associated with celiac disease. Hormonal disorders such as poorly controlled diabetes, chronic graft-versus-host disease, or reduced water intake in people undergoing hemodialysis for renal failure may also cause xerostomia due to dehydration. Xerostomia may be the result of infection with the Hepatitis C virus (HCV). A rare cause of salivary gland dysfunction may be sarcoidosis. Infection with the human immunodeficiency virus or acquired immune deficiency syndrome (AIDS) may cause a related salivary gland disease known as diffuse infiltrative lymphocytosis syndrome (DILS).

[0114] In one embodiment, the subject has bruxism, i.e. excessive grinding of teeth or jaw clenching.Generally, several symptoms are associated with bruxism, including sensitive teeth, pain in jaw muscles, headache, tooth wear, and damage to dental restorations (e.g. crowns and fillings).In this context, conventional dental care products do not necessarily reduce bruxism or jaw clenching, but serve to prevent or reduce further demineralization of teeth.

[0115] The subject may have dentin hypersensitivity. Dentin hypersensitivity is associated with a sharp, short-lasting toothache characteristically arising from exposed dentin surfaces in response to stimuli, typically thermal, evaporative, tactile, osmotic, chemical or electrical stimuli; it cannot be attributed to any other dental condition. Some degree of dentin hypersensitivity is normal, but is usually not painful during everyday activities such as drinking cold beverages.

[0116] The subject may have dental erosion. Acid erosion is a type of tooth wear. It is defined as the irreversible loss of tooth structure due to chemical dissolution by acids of non-bacterial origin. Acid erosion begins in the enamel, which thins and may progress to the dentin, giving the tooth a dull yellow appearance and resulting in dentin hypersensitivity. The most common cause of erosion is due to acidic foods and beverages. In general, foods and beverages with a pH below 2-3 are known to cause an erosive effect on the teeth. Gastroesophageal reflux disease may also be associated with erosion.

[0117] Preferably, the subject to whom the dental care product is administered has a clinical xerostomia scale of at least 1, preferably at least 4, or most preferably at least 7 on the Challacombe scale.

[0118] The dental care product of the present invention may be administered to the subject's mouth and chewed. Advantageously, it is kept in the mouth for at least 3 minutes. The dental care product may also be kept in the mouth for at least 4 minutes, preferably at least 5 minutes or at least 10 minutes.

[0119] The inventors have found that this allows at least partial separation of the two matrices of the dental treatment product, i.e. the matrix of assembled self-assembling peptides, which results in the formation of a protective layer of assembled self-assembling peptides and, for example, the gum matrix of the dental treatment product, which contributes to cleaning the teeth. The formation of the protective layer of self-assembling peptides reduces further demineralization. In contrast to increased remineralization, this effect does not depend on the presence of saliva, and therefore it is also available in subjects with reduced or absent salivary secretion.

[0120] The dental care products of the present invention that require or allow chewing, such as candies, lozenges, gelatin gums, taffy, chewing gums, biscuits, or chew toys, especially in forms related to long-term application (e.g., 5 minutes or more), such as chewing gums (for humans) or chew toys (for animals), are associated with particular advantages, since their use may simultaneously reduce biofilm or plaque, thus facilitating access to the tooth surface and potential lesions. Such products also increase saliva secretion.

[0121] Thus, preferably, upon chewing of the dental care product of the present invention, the gum base cleanses the tooth surface (e.g., it reduces the presence of pellicle and / or biofilm and / or food debris and / or calculus and / or stains, preferably all of these), and the matrix of organized self-assembling peptides provides a film to the tooth surface that reduces or prevents demineralization of the tooth surface.

[0122] It is advantageous if the dental care product is chewed, i.e. bitten, by the subject. The inventors have found that chewing the dental care product of the invention, preferably for 5 minutes, increases saliva flow by at least 10 fold.

[0123] Optionally, the dental care product further comprises a saliva stimulant, such as a parasympathomimetic agent. Thus, in addition to reducing further demineralization, the dental care product may also reduce other symptoms of xerostomia, in particular the sensation of dry mouth. However, this is not necessary in the context of the present invention.

[0124] Compared with other galenic forms of self-assembling peptides, such as toothpaste or gel, the dental care product of the present invention can be administered more easily to subjects, for example, it can be administered at work or while traveling, and it does not require the use of a washbasin or water. The ease of administration increases the compliance of subjects. Another advantage compared with toothpaste is the absence of abrasives that are typically contained in toothpaste. This is particularly beneficial for subjects who already have pathologically demineralized teeth. Furthermore, the essentially dry formulation of the dental care product of the present invention increases the stability of the product, which can be stored for a longer period of time.

[0125] The dental care product of the present invention may be administered, for example, at least once a day, preferably at least twice a day. It may also be administered at least three times a day, four times a day, or five times a day. For example, it may be administered when the subject has an increased sensation of dry mouth. The dental care product may also be administered after a meal or snack, as needed, instead of brushing the teeth. The dental care product may be administered after waking up to reduce xerostomia. Preferably, the subject applies the dental care product on a regular basis, so that further demineralization can be avoided by using the product of the present invention. For example, the dental care product may be administered for at least two consecutive days, at least three consecutive days, or at least five consecutive days. Preferably, the product is administered for at least one week, at least two weeks, at least three weeks, at least four weeks, at least one month, at least two months, at least six months, or at least one year. The product may be administered, for example, daily, for the remainder of its life.

[0126] Advantageously, the dental care product reduces the incidence of, and preferably prevents, dental caries, particularly in subjects with demineralized teeth. The dental care product of the present invention may also reduce pain associated with dentin hypersensitivity.

[0127] As used herein, "subject" refers to any subject having teeth, for example, a mammal such as a human, a feline such as a dog, a cat, a rodent such as a mouse, a rat, a hamster, a guinea pig, a cow, a horse, a camel, a sheep, a goat, or other pet, farm or zoo tooth-bearing animal. Preferably, the subject is a human.

[0128] In the context of the present invention, unless expressly stated or clear from the context, "a" is not limited to the singular, but can also mean "one or more." For example, a reference to "teeth" includes a reference to a plurality of teeth, particularly all teeth of the subject, unless expressly stated otherwise.

[0129] The following examples and embodiments are intended to illustrate, but not limit, the present invention. All references cited herein are incorporated in their entirety. [Brief description of the drawings]

[0130] Figure 1: General manufacturing flow for the preparation of coated chewing gum.

[0131] Figure 2: Typical production flow for the production of toffee. The addition of temperature sensitive ingredients may be after the online mixer.

[0132] FIG. 3: An example of a chewing gum according to the present invention.

[0133] FIG. 3A: A chewing gum product comprising an organized self-assembling peptide and a gum base, such as chicle.

[0134] FIG. 3B: Chewing gum A) Shell containing flavor B) Matrix containing organized self-assembling peptides and gum base, e.g., chicle.

[0135] FIG. 3C: Coated chewing gum: A) shell / coating containing flavors and / or self-assembling peptides. B) core containing organized self-assembling peptides and gum base matrix.

[0136] FIG 3D: Coated chewing gum: A) shell comprising organized self-assembling peptides. B) core comprising gum base, e.g. chicle. The gum base may be made from compressed gum.

[0137] FIG. 4: Example of final manufactured chewing gum containing P11-4 organized per description of compressed gum prepared according to Example 1B.

[0138] FIG. 5: Cross-section of taffy containing structured P11-4 prepared according to Example 1A with titration.

[0139] Figure 6: SEM photographs of Example 2 with protective layer. a) Intact human enamel (covered with varnish during the experiment and removed for inspection) b) Exposed human enamel with protective layer after acid erosion experiment showing reduced demineralization compared to Figure 7 proving the protective effect of the treatment. Magnification: 1000x, Signal: SE2, EHT: 10

[0140] Figure 7: kVSEM pictures of Example 2 without protective layer. a) Intact human enamel (covered with varnish during the experiment and removed for inspection) b) Exposed human enamel without protective layer after acid erosion experiment showing severe demineralization. Magnification: 1000x, Signal: SE2, EHT: 10 kV

[0141] Figure 8: TEM of reference structured P11-4. TEM, 50'000x, 50kV, AMT

[0142] FIG. 9: TEM of artificially chewed toffee saliva showing distinct fiber bundles of P11-4, see Example 3. TEM, 50'000x, 50 kV, AMT.

[0143] Figure 10: Solution prepared according to Example 1 using premix and titration. Content 35 mg * ml-1.

[0144] Figure 11: Solution prepared according to Example 1 without titration resulting in a slightly yellowish opaque solution. Content 35 mg * The concentration is ml / L.

[0145] FIG12: Device for artificial mastication of dental products. A similar device is the Dental Masticator from the University of Minnesota, shown for example at https: / / www.youtube.com / watch?v=LEJymW-g0B0.

[0146] Figure 13: Comparison of the protective effect of monomeric and polymeric self-assembling peptides. A Sample distribution on egg grid. Top left original, untreated surface protected with varnish, top right: sample 1 with polymerized self-assembling peptide. Bottom left - sample 2 with monomeric self-assembling peptide, bottom right unprotected surface. B Empty chicken egg with drawn grid, coated with Maybelline Express Finish 40s. The top square marked with a "V" is completely coated with nail polish and shows the undisturbed natural surface. For the next investigation, the remaining three squares are left blank. C Colored chicken egg with grid for sample allocation. The somewhat whitish appearance at the top left is due to the protection by the varnish, which therefore does not result in good adhesion of the color. D Incubation of eggshell in Coca Cola® in a crystallizing dish. The sites coated with the samples were balanced with 2 g of lead to ensure proper balance and complete contact to the liquid acid. E Egg 3 after 5 min incubation in Coca Cola® after rinsing with water. Top left: area protected with varnish "V", top right: polymeric P11-4 "P", bottom left: monomeric P11-4 "M", bottom right: control "C". F Example of black and white converted surface with analyzed area (thin lines). G Result of "Analyze Particle" ImageJ output. A high percentage of protected surface is seen in the erosion model for samples with intermediate protection with polymeric and monomeric self-assembling peptides, while most erosion is seen without protection, i.e. the control. Notably, no color adheres to the varnish, which explains the 0% black area shown for the varnished sample.

[0147] Working Example Example 1: Preparation of a dental care product according to the invention A) Preparation of the toffee of the invention After the ingredients are provided, a computer-based weighing system ensures that all ingredients are accurately weighed for the subsequent cooking process that produces the first matrix. Cooking is carried out at 100-150°C with stirring.

[0148] In parallel, a premix is ​​prepared. A self-assembling peptide, for example P11-4, is weighed into a suitable container. The powder is then transferred, with stirring, into another container containing a basic solvent, i.e. water adjusted to pH 8 with 0.1N NaOH solution. After addition of the self-assembling peptide, the solution is kept at basic pH for 5 minutes to ensure monomeric peptides. The solution is then slowly titrated to pH 6 using an acid, preferably phosphoric acid or citric acid 0.1N, to initiate self-assembly of the peptides. This results in a slightly opaque solution. The concentration of the peptide may be, for example, between 20-100g / L.

[0149] To meet the product requirements, the premix is ​​added continuously during the online mixing or immediately after the online mixing in the cooling tunnel. Preferably during the cooling stage with a maximum temperature of 85°C. The resulting raw toffee is rolled and formed into rods with cone rollers, followed by bar-shaped forming with leveling rollers. After this shape change, the cooled toffee is cut and wrapped online into wraps. The wrapped toffee may be packaged or stored as bulk awaiting further packaging.

[0150] Alternatively, the bulk can be prepared without titration as follows:

[0151] After the ingredients are provided, a computer-based weighing system accurately weighs all ingredients for the subsequent cooking process to produce the first matrix. Cooking is carried out at 100-150°C with stirring.

[0152] In parallel, prepare a premix. Weigh out the self-assembling peptide, for example P11-4, into a suitable container. Then, transfer the powder with stirring into another container containing an acid solution, preferably phosphoric acid or citric acid, pH 6. If necessary, correct the pH with the corresponding acid. This results in an opaque, yellowish, viscous solution. The concentration of the peptide may be, for example, between 20 and 60 g / L.

[0153] To meet the product requirements, premix is ​​added continuously during the on-line mixing or immediately after the on-line mixing in a cooling tunnel. During the cooling stage, preferably at a maximum temperature of 85°C, the resulting raw toffee is rolled and formed into rods by cone rollers and subsequently into bars by leveling rollers. After this shape change, the cooled toffee is cut and wrapped on-line into wraps. The wrapped toffee may be stored or packaged as bulk awaiting further packaging.

[0154] B) Preparation of the chewing gum of the present invention Prior to providing the materials, the SAP matrix is ​​prepared. The self-assembling peptide is weighed into a suitable container. The powder is then transferred, with stirring, into another container containing a basic solvent, i.e., water adjusted to pH 8 with 0.1N NaOH solution. After addition of the self-assembling peptide, the solution is maintained at basic pH for 5 minutes to ensure monomeric peptides. The solution is then slowly titrated to pH 6 using an acid, preferably phosphoric acid or citric acid 0.1N, to initiate self-assembly of the peptides. This results in a slightly opaque solution. The concentration of the peptides may be, for example, between 20 and 100 g / L. The solution is then dried, for example, freeze-dried or spray-dried, to preserve the fibrous structure of the matrix.

[0155] After the materials are provided, all materials are accurately weighed by a computer-based weighing system for the subsequent mixing procedure. Granulating agent, most preferably sorbitol, lubricant, such as magnesium stearate or talc, are added, and then mixed in a blender. After that, the powder of structured peptide is added, and the free-flowing powder is directly put into the press.

[0156] To improve the usefulness in terms of taste, the coating may be applied after preparation of the coating bulk, i.e. in a separate container. For this, a peptide solution may be prepared as described above. This solution is then added to flavors, colors, etc. by homogenization and is later used for spray coating of compressed gums.

[0157] After coating, the gum may be directly expanded and sealed and, if desired, further packaged.

[0158] Example 2: Acid attack test Sample preparation [Table 2]

[0159] Enamel disc preparation -Removing preferably human teeth from the refrigerator Teeth should have intact surfaces - Blot it dry -Cut off a piece of enamel -Store in PBS solution

[0160] incubation -Remove the slices from the PBS solution -Rinse under tap water -Incubate in remineralization buffer for 48 hours -Remove from solution - Applying enamel slices to the artificial teeth of the artificial chewing device with 2K adhesive -Artificial chewing taffy with or without P11-4 (33mg / ml) organized in 3mL of artificial saliva for 5 minutes Remove "saliva" A piece of enamel is incubated in "saliva" for 5 minutes. -Removing the form enamel slices from the teeth - Incubate the enamel pieces in Coca Cola for 30 minutes - Place the blotting paper -Air dry for 24 hours

[0161] SEM preparation -The sample is placed on the SEM sample holder in the form of carbon tape. - Sputter the sample in Au plasma under argon for 30 seconds. 8*10-2Pa 30sec at 20mA coating with gold The samples are analyzed in a SEM "Carl Zeiss".

[0162] Acid erosion was induced by artificially chewing enamel slices with taffy containing textured P11-4 or blank (saliva only) as described above, followed by incubation in Coca Cola for 30 min. SEM pictures of representative enamel slices with and without a protective layer formed by chewing taffy with textured P11-4 are shown in Figures 6 (no protective layer formed) and 7 (with P11-4 and protective layer). Samples with an acid protective / sacrificial layer show lower erosion than samples without protection.

[0163] Example 3: Measurement of fiber dissolution from the matrix material - P11-4 organized as a control -uL-Pipette Soccorex -Cu-TEM template 200 mesh EMS215-412-810 -Uranyl acetate 3% EMS 22400-2 Lot: 1B155953 / 131007 -EM900 TEM Zeiss

[0164] sample: -Place one taffy in 3 mL of artificial saliva with or without structured P11-4 (35 mg / ml) (see Example 2) - Chew artificially in an artificial chewing device for 5 minutes -Remove the supernatant (saliva)

[0165] Method TEM -Sample (10mg / mL) was diluted with H 2 Dilute with O -Place 1 µL of control solution on the TEM grid sample -Place 20 μL of water on the parafilm - Gently place the TEM grid with the dark area facing up on the parafilm, close to the drop. - Application of 10 μL sample onto TEM carbon-coated grids (hexagonal) -Incubation for 10 minutes - Remove unbound sample with tissue paper -Place the grid in 20 μl of 3% uranyl acetate solution -40 seconds incubation -Remove unbound uranyl acetate with tissue paper - Wash the grids twice with distilled water Pipette a 5 µL drop onto the top of the grid and remove it. - Allow to dry at room temperature for 20 minutes -Introducing the sample into the sample holder for the TEM Analyze at -50kV, vacuum of at least 9*10-6hPa

[0166] The artificial saliva contains organized fibers of the self-assembling peptide P11-4 after chewing of the toffee of the present invention, i.e. the assembled self-assembling peptide maintains its organized form after being incorporated into the toffee base and after being extracted from the same by chewing, which allows the formation of a protective sacrificial layer on the teeth that protects the teeth from erosion.

[0167] Example 4: Comparison of the protective effects of monomeric and organized self-assembling peptides against acid attack The aim of this study was to determine the protective effect of polymeric self-assembling peptides compared to monomeric self-assembling peptides based on exemplary experiments with taffy.

[0168] Due to the limited availability of human teeth, an alternative model has been established that uses chicken eggs instead of human enamel for erosion testing. Although chicken eggs are made of calcium carbonate rather than calcium phosphate as human teeth, this model is suitable to show protective effects against acidic attacks. The calcium carbonate structure of chicken eggs with a palisade structure resembles the crystalline structure of calcium phosphate in human teeth. In addition, human teeth also contain a percentage of calcium carbonate (-4%) (Klimuszko et al. 2018 Odontology 106:369-376).

[0169] The acid attack in acid erosion may be caused by acetic acid, phosphoric acid, or other organic or inorganic acids. For translation purposes, Coca Cola® was used, which is one of the widely consumed beverages that significantly lowers oral pH, with 1.7 billion servings of Coca Cola consumed daily worldwide.

[0170] The hypothesis tested in this semi-quantitative acid erosion model is whether the eggshell is better protected by the polymeric or aggregated self-assembling peptide P11-4 compared to the monomeric self-assembling peptide P11-4.

[0171] To prove this hypothesis, two sets of taffy were made and compared. The basic formula was the same, but the peptides were added in monomeric form as monomers and in polymeric form as hydrogels. The materials were added during the taffy making process, after the heating step but before the use of the mixing rod.

[0172] material and method [Table 3]

[0173] The taffy was cut and 4.2 g was weighed into a 15 mL centrifuge tube. 3 mL of isotonic NaCl solution, which mimics the natural saliva and volume present in the oral cavity, was added and the sample was placed on a rotating wheel overnight.

[0174] A raw chicken egg was carefully opened on one side and the egg white and yolk discarded. The empty chicken egg was then washed and dried. Following drying, two areas of the egg were first given a grid drawn with a fine pencil and then coated with nail polish. See Figure 13B.

[0175] After the nail polish dried, the eggs were stained with blue food dye "Brilliant Blue FCF" for better visibility by applying the color directly to the egg with a sponge on the chicken egg. A clean sponge was then used to remove excess color to ensure even distribution of the color. See Figure 13C. The partially stained eggs were then dried at 40°C for 2 hours.

[0176] After drying, the samples were assigned as defined in FIG. 13A.

[0177] 25 μL of the extracted sample or control was pipetted onto the surface of the dried egg and allowed to soak in. This process was repeated twice to ensure that the squares were completely covered.

[0178] After drying at 40°C for 1 h, the eggshells were placed in Coca Cola (pure) at 21°C and incubated for 5, 10 and 20 min as shown in Figure 13D.

[0179] After incubation, the eggshells were removed (FIG. 13E) and washed with water, followed by removal of varnish with acetone, and then air-dried.

[0180] The areas where the sample was applied were then examined under a digital USB microscope for color distribution. In areas where the acidic liquid had eaten away at the eggshell, there was less dye on the surface as the color had been eaten away along with the shell's surface.

[0181] The images were opened in ImageJ and each image was selected and converted to a binary image, i.e., the dark areas of the deep blue shell where the shell was protected were converted to black and the light areas where the shell was eroded were converted to white. An example picture is shown in Figure 13F. Within the grid, the largest possible square was analyzed using the "Analyze-Particle" function.

[0182] It should be noted that nail polish prevents the color from approaching the surface of the eggshell, so even if the shell is optimally protected under varnish, the color will be lost after washing.

[0183] result [Table 4]

[0184] conclusion The data obtained confirm the hypothesis that the polymeric self-assembling peptide P11-4 "P" provides better erosion protection compared to the monomeric self-assembling peptide P11-4 "M". The effect is highest at a short incubation time of 5 minutes, where the polymeric material protects about 20% more area compared to the monomeric form and the control "C" (Figure 13G). With longer incubation times, the effect and the difference in values ​​become smaller, but the polymeric self-assembling peptide still has a better protective effect than the monomeric self-assembling peptide, which is more protective than the control.

[0185] EMBODIMENTS OF THE PRESENTINVENTION 1. (i) a self-assembling peptide comprising the sequence of SEQ ID NO: 21, wherein the self-assembling peptide is essentially present in an assembled form (particularly at least 80%, preferably at least 90% or at least 95%); and (ii) a pharma- ceutically acceptable carrier; wherein the dental care product is an essentially solid product selected from the group consisting of chewing gum, soft chew, gelatin gum, chewing gum, chewing gum, taffy, lozenge and tablet; The dental care product is a non-abrasive dental care product. 2. The dental care product of embodiment 1, wherein the organized form of the self-assembling peptide is embedded in a pharma- ceutically acceptable base, preferably a gum base. 3. The dental care product of embodiment 2, wherein the gum base comprises a polymer, a plasticizer and / or a resin. 4. Gum base a) synthetic components selected from the group consisting of butadiene-styrene rubber, isobutylene-isoprene copolymers (butyl rubber), paraffins (produced by the Fischer-Tropsch process), petroleum-based, petroleum wax synthetic, low molecular weight elastomers such as polyisobutylene polyvinyl acetate, polyisobutadiene and isobutylene-isoprene copolymers, polybutene, polybutadiene and polyisobutylene, vinyl polymer elastomers such as polyvinyl acetate, polyethylene, vinyl copolymer elastomers such as vinyl acetate / vinyl laurate, vinyl acetate / vinyl stearate, ethylene / vinyl acetate, polyvinyl alcohol, or mixtures thereof; and / or b) natural ingredients selected from the group consisting of chicle, chiquibal, crown gum, guttahankan, masaranduba bharata, masaranduba chocolate, nispero, los diñas, Venezuelan chicle, jelutong, leche saspi, pendare, perillo, leche de vaca, niger gutta, horn, chilte, and natural rubber; 3. The dental care product of embodiment 2, comprising: 5. The dental care product of any of the previous embodiments comprising gelatin, egg white, lecithin, pectin or starch. 6. The dental care product of any of the previous embodiments, wherein the concentration of the self-assembling peptide is 0.1-500 mg / kg, preferably about 5-15 mg / kg. 7. The dental care product of any of the previous embodiments, wherein said peptide comprises the sequence of SEQ ID NO:22. 8. The dental care product of any of the previous embodiments, wherein said peptide comprises the sequence of SEQ ID NO:23. 9. The dental care product of any of the previous embodiments, wherein said peptide comprises the sequence of SEQ ID NO:24. 10. The dental care product of any of the previous embodiments, wherein said peptide comprises the sequence of SEQ ID NO:25. 11. The dental care product of any of the previous embodiments, wherein said peptide comprises the sequence of SEQ ID NO:26. 12. The dental care product of any of the previous embodiments, wherein the peptide comprises the sequence of any one of SEQ ID NOs: 1-20. 13. The dental care product of any of the previous embodiments, wherein the self-assembling peptide comprises a sequence having at least 80% sequence identity to one of the sequences of SEQ ID NO:1, 2 or 20, wherein preferably the peptide comprises the sequence of SEQ ID NO:1. 14. The dental care product of embodiment 13, wherein the self-assembling peptide comprises a sequence having at least 80% sequence identity to SEQ ID NO:1, preferably wherein the peptide comprises the sequence of SEQ ID NO:1. 15. The dental care product of embodiment 13, wherein the self-assembling peptide comprises a sequence having at least 80% sequence identity to SEQ ID NO:3, preferably wherein the peptide comprises the sequence of SEQ ID NO:3. 16. The dental care product of embodiment 13, wherein the self-assembling peptide comprises a sequence having at least 80% sequence identity to SEQ ID NO:20, preferably wherein the peptide comprises the sequence of SEQ ID NO:20. 17. The dental care product of any of embodiments 1-15, wherein the peptide is capable of self-assembly at a pH below 7.5. 18. The dental care product of any of embodiments 1-6, 12 or 16, wherein the peptides are capable of undergoing self-assembly at a pH above 7.5. 19. The dental care product of any of the preceding embodiments does not contain more than 0.4% by weight of mineral particles having a size of at least 0.1 μm. 20. The dental care product of any of the previous embodiments, wherein the dental care product further comprises a) a polyol, such as xylitol, erythritol or sorbitol, preferably xylitol. 21. The dental care product of any of the previous embodiments, wherein the dental care product further comprises b) a phosphate salt, such as sodium phosphate, calcium phosphate, e.g. hydroxyapatite. 22. The dental care product of any of the previous embodiments, wherein the dental care product further comprises c) a pyrophosphate salt. 23. The dental care product of any of the previous embodiments, wherein the dental care product further comprises d) a pH control agent, such as baking soda or urea. 24. a) Providing an organized matrix of self-assembling peptides b) providing a pharma- ceutically acceptable base, preferably a gum base, where steps a) and b) can be performed in any order; c) homogenizing the matrix of assembled self-assembling peptides and a pharma- ceutically acceptable base, preferably a gum base, optionally together with other ingredients; d) forming dental care products; and e) Packaging dental care products, if desired. A method for preparing the dental care product of any of embodiments 1-23, comprising the steps of: 25. The process of embodiment 24, wherein the matrix of step a is prepared by drying, for example by spray drying, freeze drying or evaporation, a solution having a pH at which the self-assembling peptide assembles (e.g., a pH of less than 7.5 for the peptide of SEQ ID NO: 22). 26. The dental care product of any of embodiments 1-23, obtainable by the method of any of embodiments 22 or 23. 27. The dental care product of any of embodiments 1-23 or 26 for use in reducing demineralization of a tooth surface in a subject having demineralized teeth, preferably for reducing further demineralization of a tooth surface in a subject having demineralized teeth. 28. The dental treatment product for use according to embodiment 27, wherein the subject has a disease or condition associated with reduced remineralization of teeth. 29. The dental care product for use according to any of embodiments 27 or 28, wherein the subject has xerostomia, hyposalivation, bruxism, dentin hypersensitivity and / or acid erosion. 30. The dental care product for use in any of embodiments 27-29, wherein the subject has xerostomia. 31. The dental care product for use according to embodiment 30, wherein the xerostomia is associated with reduced saliva secretion. 32. Dental care product for use in any of embodiments 27-31, wherein the subject has low saliva production. 33. A dental care product for use in any of embodiments 27-32, wherein the subject has bruxism. 34. Dental care product for use in any of embodiments 27-33, wherein the subject has acid erosion. 35. The dental treatment product for use in embodiment 34, wherein the subject has gastroesophageal reflux disease. 36. A dental treatment product for use in any of embodiments 27-35, wherein the subject has dental hypersensitivity. 37. The dental care product for use in any of embodiments 27-36, wherein the subject has a clinical xerostomia scale of at least 1, preferably at least 4 or at least 7 on the Characombe scale. 38. The dental care product for use of any of embodiments 27-37, wherein the dental care product is administered to the subject's mouth, and preferably wherein the dental care product is maintained in the mouth for at least 3 minutes. 39. A dental care product for use according to any of embodiments 26-37, wherein the dental care product is chewed by the subject, preferably chewing for 5 minutes increases saliva flow by at least 10 times. 40. A dental care product for use of any of embodiments 27-39, wherein upon chewing, the gingival base cleanses the tooth surface and the matrix of organized self-assembling peptides provides a film on the tooth surface that reduces or prevents further demineralization of the tooth surface. 41. A dental care product for use according to any of embodiments 27-40, wherein the dental care product is administered at least once a day, preferably at least twice a day. 42. A dental care product for use according to any of embodiments 27-41, wherein the dental care product is optionally administered after a meal or snack instead of brushing the teeth. 43. A dental care product for use according to any of embodiments 27-42, wherein the dental care product is administered after waking up to relieve xerostomia. 44. A dental care product for use according to any of embodiments 27-43, wherein the dental care product reduces the incidence and preferably prevents dental caries. 45. A dental treatment product for use according to any of embodiments 27-44, for relieving pain associated with dentin hypersensitivity.

Claims

1. (i) a self-assembling peptide comprising the sequence of SEQ ID NO:21, wherein the self-assembling peptide is present in the dental care product in at least 80% assembled form; and (ii) a pharma- ceutically acceptable carrier wherein the dental care product is an essentially solid product selected from the group consisting of chewing gum, soft chew, taffy, gelatin gum, chewing gum, chewing gum, lozenge, or tablet; The dental care product is a non-abrasive dental care product.

2. 2. The dental care product of claim 1, wherein the self-assembling peptide in an organized form is embedded in a pharma- ceutically acceptable base, which is preferably a gum base comprising components selected from the group comprising polymers, plasticizers and / or resins.

3. The gum base is a) synthetic components selected from the group consisting of butadiene-styrene rubber, isobutylene-isoprene copolymers, paraffins, petroleum-based (way), petroleum wax synthetics, low molecular weight elastomers such as polyisobutylene polyvinyl acetate, polyisobutadiene and isobutylene-isoprene copolymers, polybutene, polybutadiene and polyisobutylene, vinyl polymeric elastomers such as polyvinyl acetate, polyethylene, vinyl copolymer elastomers such as vinyl acetate / vinyl laurate, vinyl acetate / vinyl stearate, ethylene / vinyl acetate, polyvinyl alcohol, or mixtures thereof; and / or b) natural ingredients selected from the group consisting of chicle, chiquibal, crown gum, guttahankan, masaranduba bharata, masaranduba chocolate, nispero, los diñas, Venezuelan chicle, jelutong, leche saspi, pendare, perillo, leche de vaca, nigergutta, horn, chirte, and natural rubber; 3. The dental care product of claim 2 comprising:

4. 4. A dental care product according to any one of claims 1 to 3, comprising gelatin, pectin, egg white, lecithin, maltitol / sorbitol or starch.

5. A dental care product according to any one of claims 1 to 4, wherein the concentration of the self-assembling peptide is 0.1-5000 mg / kg, preferably about 5-15 mg / kg.

6. 6. A dental care product according to any one of claims 1 to 5, wherein the peptide comprises the sequence of SEQ ID NO:

22.

7. 7. A dental care product according to any one of claims 1 to 6, wherein the self-assembling peptide comprises a sequence having at least 80% sequence identity to one of the sequences of SEQ ID NO: 1, 3 or 20, preferably wherein the peptide comprises the sequence of SEQ ID NO:

1.

8. 8. A dental care product according to any one of claims 1 to 7, which does not contain more than 0.4% by weight of mineral particles having a size of at least 0.1 μm, Wherein, optionally, the dental care product comprises: a) a polyol selected from the group comprising xylitol, erythritol and sorbitol; b) a salt selected from the group comprising calcium carbonate and phosphates, the salt having a MOSH hardness of less than 3; c) pyrophosphate, and d) pH control agent 13. A dental care product comprising at least one further ingredient selected from the group comprising:

9. A method for preparing a dental care product according to any one of claims 1 to 8, comprising the steps of: a) providing a matrix of organized self-assembling peptides; b) providing a pharma- ceutically acceptable base, preferably a gum base, where steps a) and b) can be performed in any order; c) homogenizing the matrix of assembled self-assembling peptides and the pharma- ceutically acceptable carrier, optionally together with other ingredients; d) forming a dental care product; and e) Optionally, packaging dental care products. A method comprising:

10. 9. A dental care product according to any one of claims 1 to 8 for use in reducing further demineralisation of the dental surfaces of a subject having demineralised teeth comprising orally administering the dental care product to the mouth of the subject, wherein preferably the dental care product is maintained in the mouth for at least 3 minutes.

11. 11. A dental care product for use according to claim 10, wherein the subject has a disease or condition associated with reduced tooth remineralisation selected from the group comprising xerostomia, hyposalivation, bruxism, dentin hypersensitivity and / or acid erosion, optionally acid erosion associated with hyposalivation.

12. 12. A dental care product for use according to any one of claims 10 or 11, wherein the dental care product is to be chewed by the subject, preferably chewing for 5 minutes increases saliva flow by at least 10 times.

13. 13. A dental care product for use according to any one of claims 10-12, wherein upon chewing, the gum base cleanses the tooth surface and the matrix of organized self-assembling peptides provides a film on the tooth surface which reduces or prevents further demineralization of the tooth surface.

14. A dental care product for use according to any one of claims 10-13, wherein said dental care product reduces the incidence of dental caries.

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