Skin dullness improver

Abstract

To provide a loricrin expression enhancer, a stratum corneum formation promoter, and a skin dullness improver which promote loricrin expression.SOLUTION: A loricrin expression enhancer comprises Eucalyptus globulus or its extract, and a plant of the Vaccinium genus in the Ericaceae family or its extract as active ingredients.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a loricrin expression promoter, a stratum corneum formation promoter, and an agent for improving skin dullness. [Background technology]

[0002] In order to improve the skin's ability to retain moisture, prevent and improve rough skin, and prevent and improve skin aging such as the formation of wrinkles and the reduction of texture, it is believed that it is effective to act on the epidermal cells of the skin, promote the keratinization of the epidermal cells, promote the formation of a healthy stratum corneum, and improve the stratum corneum barrier function to protect the body from external stimuli and other factors. In addition, the formation of a healthy stratum corneum also leads to the improvement of dull skin (Non-Patent Document 1). Dull skin is a phenomenon that occurs on the entire face or on areas such as around the eyes and cheeks, in which the skin's transparency decreases and it appears dark, with unclear boundaries. There are several thought to be factors that cause dull skin, one of which is a decrease in transparency (light transmittance) due to thickening of the stratum corneum (Non-Patent Document 2).

[0003] Thickening of the stratum corneum refers to a state in which the stratum corneum is thicker than normal, and is also called stratum corneum stratification. The cause of thickening of the stratum corneum is thought to be the disruption of turnover due to the effects of aging, dryness, ultraviolet rays, etc., which causes abnormalities in the formation and peeling of stratum corneum cells. Turnover here refers to the continuous repetition of the proliferation of keratinocytes (epidermal keratinocytes) in the basal layer, the process of keratinization, and the peeling of the stratum corneum. During the keratinization process, these keratinocytes form each layer, changing sequentially from basal cells, spinous cells, granular cells, and stratum corneum cells toward the outside. Then, proteins that make up the cornified envelope (CE) are synthesized from the upper spinous layer to the granular layer. Furthermore, in the process of reaching the stratum corneum, matrix proteins such as involucrin, loricrin, and cystatin are bound to the cell membrane of keratinocytes by the enzyme transglutaminase, forming insoluble CE. Furthermore, ceramide and other substances are covalently bound to the insoluble CE, forming the basis of the stratum corneum barrier function. Eventually, as they reach the surface layer of the stratum corneum, the adhesive strength of the keratinocytes weakens and they become scum and fall off. Therefore, the abnormal formation and peeling of the stratum corneum in the turnover disorder leads to abnormal parakeratinization, which is one of the causes of thickening of the stratum corneum. Conventionally, skin troubles caused by the abnormal turnover disorder have been solved by creams containing ceramide. However, the improvement effect of promoting keratinization of epidermal cells and promoting the health of the stratum corneum is not sufficient, so the development of ingredients that promote the formation of a healthy stratum corneum is desired.

[0004] Among the above matrix proteins, loricrin is a major component of CE and is involved in various stratum corneum functions, including the formation of natural moisturizing factors. Diseases caused by loricrin gene mutations are collectively called loricrin keratosis, and symptoms such as diffuse keratinocyte thickening accompanied by abnormal keratinization are observed (Non-Patent Document 3). It has also been reported that transfection of the loricrin gene into HaCaT cells, a cell line of human epidermal keratinocytes, leads to programmed cell death associated with terminal keratinization, and that loricrin is an important molecule for normal keratinization (Non-Patent Document 4). Therefore, it is believed that promoting the expression of loricrin in epidermal cells promotes normal keratinization of keratinocytes and the formation of a healthy stratum corneum, improving stratum corneum thickening, and as a result, improving dull skin. In recent years, loricrin has been attracting attention as a differentiation marker for epidermal cells.

[0005] On the other hand, it has been reported that extracts from Eucalyptus globulus, a member of the Myrtaceae family, have the effect of promoting stratum corneum formation by promoting involucrin expression, and are useful for improving the skin's ability to retain moisture (moisturizing effect) and reducing the visibility of pores (Patent Document 1). In addition, it has been reported that an extract of bilberry, which belongs to the genus Vaccinium of the Ericaceae family, improves abnormal mineral metabolism in the lower part of the epidermis and normalizes epidermal cell proliferation and epidermal turnover (Patent Document 2). However, there have been no reports on the effect of combined use of Eucalyptus and a plant of the genus Vaccinium of the Ericaceae family on loricrin expression. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] JP 2007-277149 A [Patent Document 2] JP 2021-155410 A [Non-patent literature]

[0007] [Non-Patent Document 1] T. Ishida et al. J. Soc. Cosmet. Chem. Jpn. 54 (3): 258-63 (2020) [Non-Patent Document 2] Masako Naganuma Journal of the Japanese Society of Cosmetic Science Vol. 39, No. 4, pp. 275-285 (2015) [Non-Patent Document 3] A. I-Yamamoto. J. Dermatol. Sci. 31 (1): 3-8 (2003) [Non-Patent Document 4] K. Yoneda et al. J. Dermatol. 37 (11): 956-964 (2010) Summary of the Invention [Problem to be solved by the invention]

[0008] The present invention relates to providing a loricrin expression promoter, a stratum corneum formation promoter, and an agent for improving skin dullness, which promote the expression of loricrin. [Means for solving the problem]

[0009] The present inventors have conducted intensive research in light of the above-mentioned problems and have found that combining eucalyptus extract with an extract of a plant of the genus Vaccinium in the family Ericaceae synergistically enhances loricrin protein expression, and is useful for promoting stratum corneum formation and improving skin dullness.

[0010] That is, the present invention relates to the following 1) to 3). 1) A loricrin expression promoter having as active ingredients eucalyptus or an extract thereof, and a plant of the genus Vaccinium of the Ericaceae family or an extract thereof. 2) A stratum corneum formation promoter having as active ingredients eucalyptus or its extract, and a plant of the genus Vaccinium of the Ericaceae family or its extract. 3) A skin dullness improving agent containing eucalyptus or its extract, and a plant of the genus Vaccinium of the Ericaceae family or its extract as active ingredients. Effect of the Invention

[0011] According to the present invention, by promoting the expression of loricrin, it is possible to promote the formation of a healthy stratum corneum and improve dull skin. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] In this specification, "eucalyptus" refers to Eucalyptus globulus, which belongs to the genus Eucalyptus in the family Myrtaceae. "Plants of the genus Vaccinium in the family Ericaceae" refers to plants belonging to the genus Vaccinium in the family Ericaceae, including, for example, bilberry, cranberry, blueberry, cowberry (cowberry, lingonberry), huckleberry, and shashambo. One or more species of plants of the genus Vaccinium in the family Ericaceae can be used. Among these, bilberry (Vaccinium myrtillus L.) is preferred. Examples of the parts of the plants that can be used include the whole plant, whole tree, leaves, stems, buds, flowers, buds, trees, woody parts, bark, roots, rhizomes, pseudocorms, tuberous roots, lichens, thallus, seeds, fruits, resins, and mixtures thereof. In the case of Eucalyptus, the parts of the plants that can be used are preferably leaves, flowers, and fruits, and more preferably leaves. In the case of Vaccinium plants of the Ericaceae family, the parts of the plants that can be used are preferably leaves, flowers, and fruits, and more preferably leaves.

[0013] Such plants can be used as they are or as juice obtained by squeezing them, as a dried product obtained by drying the plant body itself or its pulverized product, or as an extract extracted from these, but it is preferable to use them as an extract.

[0014] Examples of the extract include various solvent extracts obtained by extracting the above-mentioned plants at room temperature or under heating, or by using an extraction device such as a Soxhlet extractor, extracts obtained by supercritical extraction using supercritical carbon dioxide or the like, dilutions thereof, concentrates thereof, and dried powders thereof. The extraction means is not particularly limited, but may be any of the usual means such as soaking, decoction, percolation, reflux extraction, ultrasonic extraction, microwave extraction, stirring, etc.

[0015] The solvent for extraction may be either a polar solvent or a non-polar solvent. Specific examples of the solvent include water; monohydric, dihydric or polyhydric alcohols; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; linear or cyclic ethers such as diethyl ether and tetrahydrofuran; polyethers such as polyethylene glycol; saturated or unsaturated hydrocarbons such as hexane; aromatic hydrocarbons such as benzene and toluene; halogenated hydrocarbons such as dichloromethane, chloroform, dichloroethane and carbon tetrachloride; pyridines; dimethyl sulfoxide; acetonitrile; carbon dioxide, supercritical carbon dioxide; oils and fats, waxes, other oils; and mixtures thereof. Suitable examples include water, alcohols and aqueous solutions thereof, and examples of alcohols include methanol, ethanol, 1,3-butylene glycol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, t-butanol, etc., and preferably ethanol and 1,3-butylene glycol, and more preferably 1,3-butylene glycol.

[0016] The alcohol concentration (volume percentage at 25°C, hereinafter referred to as % (v / v)) in the aqueous solution of the above alcohols is preferably 30% (v / v) or more, more preferably 40% (v / v) or more, even more preferably 45% (v / v) or more, and is preferably 99.8% (v / v) or less, more preferably 90% (v / v) or less, even more preferably 85% (v / v) or less.

[0017] The amount of the solvent used in the extraction is preferably 1 to 100 mL, and more preferably 3 to 50 mL, per 1 g of the plant (calculated as a dry mass). The extraction conditions are not particularly limited as long as sufficient extraction can be performed, but for example, the extraction time is preferably 1 hour or more, more preferably 3 hours or more, and is preferably 2 months or less, more preferably 5 weeks or less, and more preferably 3 weeks or less. The extraction temperature is preferably 0° C. or higher, more preferably 5° C. or higher, and is preferably the boiling point of the solvent or lower, more preferably 90° C. or lower. In general, extraction is performed for a long time at a low temperature and for a short time at a high temperature.

[0018] The extract may be a crude product as long as it meets the standards acceptable for cosmetics and pharmaceuticals and exerts the effects of the present invention. If necessary, the extract may be subjected to treatments such as removal of inactive contaminants, deodorization, decolorization, etc., by known techniques such as liquid-liquid distribution, solid-liquid distribution, filtration membrane, activated carbon, adsorption resin, ion exchange resin, and precipitation. The purity of these compounds may be further increased by appropriately combining known separation and purification methods, such as organic solvent precipitation, centrifugation, ultrafiltration membrane, high performance liquid chromatography, and column chromatography.

[0019] The extract may be used as it is, or may be used as a dilution solution diluted with an appropriate solvent, or may be prepared as a concentrated extract, dried powder, or paste. It may also be freeze-dried and diluted with a solvent normally used in extraction, such as water, ethanol, 1,3-butylene glycol, a mixture of water and ethanol, or a mixture of water and 1,3-butylene glycol, before use. It may also be encapsulated in a vesicle such as a liposome or a microcapsule.

[0020] As shown in the examples below, the combination of eucalyptus extract and bilberry extract promotes the expression of loricrin. Moreover, the promoting effect is superior to the effect of each of eucalyptus extract and bilberry extract alone, and exceeds the additive effect when each is added alone, resulting in a synergistic effect. As described above, promoting the expression of loricrin promotes normal keratinocyte keratinization and healthy stratum corneum formation. This is expected to improve dull skin. Therefore, a combination of eucalyptus or an extract thereof and a plant of the genus Vaccinium of the Ericaceae family, such as bilberry, or an extract thereof, can serve as a loricrin expression promoter, a stratum corneum formation promoter, and an agent for improving skin dullness (hereinafter also referred to as "loricrin expression promoters, etc."), and can be used to promote the expression of loricrin, promote the formation of the stratum corneum, and improve skin dullness, and can also be used to produce loricrin expression promoters, etc. Here, "use" may be for humans or non-human animals, and may be therapeutic or non-therapeutic. "Non-therapeutic" is a concept that does not include medical procedures, i.e., methods of surgery, treatment, or diagnosis of humans, and more specifically, does not include methods of surgery, treatment, or diagnosis performed by a physician, a medical professional, or a person under the instruction of a physician. In the present invention, non-therapeutic use includes the use of the above-mentioned plants or extracts thereof for cosmetic or aesthetic purposes.

[0021] As used herein, "promotion of loricrin expression" encompasses promotion of loricrin expression at the gene level and promotion of loricrin expression at the protein level. Promotion of expression at the gene level includes promotion of transcription into mRNA in the expression of mRNA encoding loricrin, and promotion of expression at the protein level includes promotion of mRNA translation, etc. Of these, promotion of expression at the protein level is preferred in the present invention. Human loricrin is composed of 315 amino acids and has a molecular weight of approximately 26 kDa.

[0022] "Promotion of stratum corneum formation" means that normal keratinization of keratinocytes is promoted, and the formation of a healthy stratum corneum (horny layer) is promoted. Here, a healthy stratum corneum means a stratum corneum with healthy skin turnover, specifically a stratum corneum in which the proliferation of keratinocytes in the basal layer and the peeling of the stratum corneum from the surface layer are balanced, and no thickening of the stratum corneum is observed.

[0023] "Dull skin" refers to a state in which the skin appears dark due to reduced transparency caused by thickening of the stratum corneum, etc. Areas where dull skin occurs include, for example, the entire face, around the eyes, cheeks, forehead, neck, back of the hands, back of the feet, etc. "Improvement" refers to improvement of the condition, prevention or delay of deterioration of the condition, or reversal, prevention or delay of the progression of deterioration of the condition.

[0024] In the present invention, Eucalyptus or an extract thereof, and a plant of the genus Vaccinium of the Ericaceae family or an extract thereof may be administered in either order, or may be administered simultaneously. When the two agents are not administered simultaneously, the administration interval between the two agents may be appropriately selected as long as the effect of enhancing the loricrin expression promoting action of Eucalyptus or an extract thereof, or a plant of the genus Vaccinium of the Ericaceae family or an extract thereof is exhibited. An agent comprising a combination of eucalyptus or an extract thereof, and a plant of the genus Vaccinium of the Ericaceae family or an extract thereof may be formulated into a single formulation as a combination agent, or may be a kit in which the individual formulations can be used simultaneously or separately at intervals.

[0025] The loricrin expression promoter etc. of the present invention may be a drug, quasi-drug, or cosmetic itself for promoting the expression of loricrin, promoting the formation of the stratum corneum, or improving dullness of the skin, or may be a material or preparation to be incorporated into the drug, quasi-drug, or cosmetic.

[0026] The pharmaceutical product (including quasi-drugs, the same applies below) contains eucalyptus or its extract, and a plant of the genus Vaccinium of the Ericaceae family or its extract as active ingredients for promoting loricrin expression, promoting the formation of the stratum corneum, and improving dullness of the skin. Furthermore, the pharmaceutical product may contain a pharma- ceutically acceptable carrier, or other active ingredients, medicinal ingredients, etc., as necessary, so long as the functions of the active ingredients are not lost. The administration form of the pharmaceutical product is arbitrary, but is preferably parenteral administration.The dosage form for parenteral administration includes each preparation for external use on the skin, transdermal, transmucosal, nasal, enteral, injection, suppository, injection, inhalation, patch, etc. Among them, the preferred preparation form is an external use on the skin, specifically, ointment, emulsion, cream, milky lotion, lotion, gel, aerosol, etc.

[0027] The cosmetic contains eucalyptus or an extract thereof, and a plant of the genus Vaccinium of the Ericaceae family or an extract thereof as active ingredients for promoting loricrin expression, promoting the formation of the stratum corneum, and improving dullness of the skin. Furthermore, the cosmetic may contain a carrier acceptable for cosmetics, or other active ingredients, cosmetic ingredients, etc., as necessary, so long as the functions of the active ingredients are not lost. Preferred examples of cosmetics include face and body cosmetics (for example, lotions, gels, creams, packs, etc.), make-up cosmetics, face or body cleansers, and the like.

[0028] Each of these pharmaceutical and cosmetic preparations can be manufactured according to conventional methods by combining eucalyptus or an extract thereof, and a plant of the genus Vaccinium of the Ericaceae family or an extract thereof, as necessary, with a pharma- ceutical or cosmetically acceptable carrier, the other active ingredients, medicinal ingredients, cosmetic ingredients, etc. described above. Examples of the pharma- ceutically or cosmetically acceptable carriers include various oils, surfactants, gelling agents, buffers, preservatives, antioxidants, solvents, dispersants, chelating agents, thickeners, UV absorbers, emulsion stabilizers, pH adjusters, colorants, and fragrances. Examples of such other active ingredients, medicinal ingredients, and cosmetic ingredients include plant extracts, bactericides, moisturizers, anti-inflammatory agents, antibacterial agents, keratolytic agents, cooling agents, antiseborrheic agents, cleansing agents, and makeup ingredients.

[0029] The content of eucalyptus or an extract thereof in the above pharmaceutical or cosmetic formulations cannot be generalized as it varies depending on the form of the formulation. For example, based on the total amount of the formulation, the content is preferably 0.00006% by mass or more, more preferably 0.0001% by mass or more, even more preferably 0.0002% by mass or more, calculated as solid content, and is preferably 0.006% by mass or less, more preferably 0.002% by mass or less, even more preferably 0.0006% by mass or less. Furthermore, the content of a plant of the genus Vaccinium of the Ericaceae family or an extract thereof in the above-mentioned pharmaceutical or cosmetic formulations cannot be generalized because it varies depending on the form of the formulation; however, for example, based on the total amount of the formulation, the content is, calculated as solid content, preferably 0.00002 mass% or more, more preferably 0.00006 mass% or more, even more preferably 0.0001 mass% or more, and is preferably 0.002 mass% or less, more preferably 0.0006 mass% or less, even more preferably 0.0002 mass% or less.

[0030] In the loricrin expression promoters, etc. of the present invention, the ratio of Eucalyptus or an extract thereof to a plant of the genus Vaccinium of the Ericaceae family or an extract thereof, in terms of promoting loricrin expression and promoting stratum corneum formation, is preferably 0.01 or more, more preferably 0.1 or more, even more preferably 1 or more, in terms of the mass ratio [converted into solids content of Eucalyptus or an extract thereof / converted into solids content of a plant of the genus Vaccinium of the Ericaceae family or an extract thereof], and is preferably 300 or less, more preferably 30 or less, and even more preferably 4 or less.

[0031] The dosage or use amount of Eucalyptus or its extract may be an amount that can achieve the effects of the present invention. The dosage or use amount may vary depending on the species, weight, sex, age, condition, or other factors of the subject, but in the case of parenteral administration such as external skin preparations, the dosage or use amount is preferably 0.0012 μg / cm as the mass of Eucalyptus or its extract in terms of solid content once per adult (60 kg). 2 More preferably, 0.002 μg / cm 2 More preferably, 0.004 μg / cm 2 or more, and preferably 0.12 μg / cm 2 Less than or equal to 0.04 μg / cm 2 Less than 0.012 μg / cm 2 The dosage or use amount of the plant of the genus Vaccinium of the Ericaceae family or an extract thereof is preferably 0.0004 μg / cm3 in terms of the mass of the plant of the genus Vaccinium of the Ericaceae family or an extract thereof in terms of solid content once per adult (60 kg). 2 More preferably, 0.0012 μg / cm 2 More preferably, 0.002 μg / cm 2 or more, and preferably 0.04 μg / cm 2 Less than or equal to 0.012 μg / cm 2 Less than 0.004 μg / cm 2 The following is the result. In the present invention, such an amount can be administered or used repeatedly or continuously, once or in divided doses per day, for one day or more, preferably seven days or more, more preferably 14 days or more, and even more preferably 42 days or more.

[0032] The subject to which the loricrin expression promoter of the present invention is administered or used is not particularly limited as long as it is a human or a non-human animal that requires or desires it. Preferred examples of subjects include humans who desire promotion of loricrin expression, promotion of stratum corneum formation, and improvement of dull skin. Non-human animals include non-human mammals such as apes and other primates. Furthermore, the site to which the loricrin expression promoter of the present invention is administered or used is not particularly limited, but preferred examples include the face, neck, arms, backs of the hands, fingers, legs, and backs of the feet. EXAMPLES

[0033] Example 1: Loricrin expression promoting effect plant extract The eucalyptus extract used was obtained by extracting eucalyptus leaves with an 80% (v / v) 1,3-butylene glycol aqueous solution (Maruzen Pharmaceutical Co., Ltd., Eucalyptus Extract BG-KA, solids concentration 0.2% (w / v)). The bilberry extract used was obtained by extracting bilberry leaves with a 50% (v / v) 1,3-butylene glycol aqueous solution (Ichimaru Pharcos Co., Ltd., Cureberry (registered trademark), solids concentration 0.2% (w / v)).

[0034] Experimental cell culture The cells used were normal human epidermal keratinocytes (derived from neonatal foreskin; Kurabo). For cell proliferation and culture, EpiLife Medium, with 60 μM calcium (Thermo Fisher) was supplemented with HKGS (Thermo Fisher). For material supplementation, EpiLife Medium, with 60 μM calcium was supplemented with HKGS Kit (BPE and EGF-free; Thermo Fisher). Cell culture was performed at 37°C and 5% CO. 2 The experiment was carried out according to a conventional method under the following conditions.

[0035] Protein extraction and loricrin expression analysis Normal human epidermal keratinocytes were cultured in a 6-well plate (collagen-coated; Corning) at 1.5 × 10 5The cells were seeded at a density of 100 cells / well, cultured in a growth medium for one day, then replaced with a medium containing the material and cultured for another day. The next day, eucalyptus extract dissolved in 80% (v / v) 1,3-butylene glycol (added at a concentration of 0.3% (v / v)) and bilberry extract dissolved in 50% (v / v) 1,3-butylene glycol (added at a concentration of 0.1% (v / v)) were added, and the cells were cultured for 48 hours, washed once with PBS, dissolved in RIPA buffer (SIGMA) containing Protease / Phosphatase Inhibitor Cocktail (Cell Signaling) diluted 100 times, and the cells were collected. The cell suspension was homogenized (ultrasonicated), centrifuged (15,000×g, 10 min), and the supernatant was collected. Protein quantification was then performed using Pierce (registered trademark) BCA Protein Assay Kit (Thermo Fisher). 8 to 10 μg of protein was required for detection. The samples were separated by electrophoresis using NuPAGE® 4-12% SDS (sodium dodecyl sulfate)-polyacrylamide gel (Thermo Fisher), transferred to a PVDF membrane, blocked for 1 hour with TBS-T (Tween 20 0.1% (w / v)) containing 5% (w / v) skim milk, and incubated overnight at 4°C with a primary antibody (anti-Loricrin antibody; Abcam) diluted 2000-fold in blocking solution. After washing three times for 15 minutes with PBS-T, the samples were incubated for 1 hour at room temperature with a secondary antibody (anti-rabbit IgG antibody (HRP labeling; Dako) diluted 4000-fold in blocking solution. After washing three times for 10 minutes with PBS-T, the samples were incubated for 1 hour at room temperature with the luminescence reagent SuperSignal TM Images were taken using a detector (Amersham Imager 600) with West Dura Extended Duration substrate (Thermo Fisher). Band density was quantified using an Amersham Imager 600. The measurement data was evaluated relative to the amount of loricrin protein expression, with the value of the control group set at 1. The results are shown in Table 1.

[0036] [Table 1]

[0037] As is clear from Table 1, it was confirmed that in the group to which eucalyptus extract and bilberry extract were added in combination, the expression of loricrin was synergistically promoted more than in the groups to which eucalyptus extract or bilberry extract was added alone.

Claims

1. A loricrin expression promoter containing eucalyptus (Eucalyptus globulus) leaf extract and bilberry (Vaccinium myrtillus L.) leaf extract as active ingredients.

2. A stratum corneum formation promoter containing eucalyptus (Eucalyptus globulus) leaf extract and bilberry (Vaccinium myrtillus L.) leaf extract as active ingredients.

3. A skin dullness improving agent containing eucalyptus (Eucalyptus globulus) leaf extract and bilberry (Vaccinium myrtillus L.) leaf extract as active ingredients.

4. The agent according to any one of claims 1 to 3, wherein the mass ratio of the eucalyptus (Eucalyptus globulus) leaf extract to the bilberry (Vaccinium myrtillus L.) leaf extract (solid content equivalent of eucalyptus (Eucalyptus globulus) leaf extract / solid content equivalent of bilberry (Vaccinium myrtillus L.) leaf extract) is 0.1 or more and 300 or less.

5. The agent according to any one of claims 1 to 3, which is in the form of an external preparation for skin.

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