Method for evaluating earth and sand utilization and method for utilizing earth and sand

Abstract

To provide methods for analyzing earth and sand that are possible to evaluate the utilization of the entire earth and sand to be utilized, based on the presence or absence of a target organism, and the like, and to provide methods for evaluating the utilization of earth and sand.SOLUTION: The method of analyzing earth and sand targets the earth and sand to be utilized as an evaluation target comprises: step S01 of putting the earth and sand to be evaluated into a storage tank; step S03 of stirring the earth and sand in the storage tank; and step S07 of collecting water from the storage tank and analyzing for the water the environmental DNA derived from a target organism.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a method for analyzing earth and sand to be utilized. by An evaluation method for earth and sand utilization and method of using earth and sand and relates thereto.

Background Art

[0002] Conventionally, in order to detect and analyze organisms contained in earth and sand, construction-generated soil, dredged soil, etc. (hereinafter, for convenience, referred to as "earth and sand"), after separating from the earth and sand to be analyzed (including sieving and the Tullgren method in the case of soil animals), visual inspection is performed, or a sample is collected, and DNA is extracted and analyzed. For example, in the extraction operation in the laboratory, DNA is transferred from a small amount of earth and sand to water.

[0003] Patent Document 1 discloses a method for extracting DNA of microorganisms in soil, which instantaneously freezes soil containing microorganisms, freeze-dries the instantaneously frozen soil, and then pulverizes it to extract DNA of microorganisms contained in the soil. Patent Document 2 discloses a pretreatment method for an environmental water sample for estimating the biological phase or the amount of a specific organism at the sampling location of the environmental water sample by analyzing nucleic acids contained in the environmental water sample collected from a predetermined environment.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Non-Patent Documents

[0005]

Non-Patent Document 1

Non-Patent Document 2

Non-Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0006] When specific organisms or the like are contained in sediment, it may prevent the effective use or wide-area use of the sediment. For example, when the dredged soil contains the spider Kaiyadori Ummigumo that parasitizes bivalves, or the oyster drill that damages clams such as Asari, it is expected to be an obstacle when using the dredged soil as a material for creating tidal flats and shallow areas. In addition, when using sediment containing alien organisms (including specified alien organisms) such as insects and plants, there is a risk of disturbing the ecosystem at the destination.

[0007] In order to avoid the above adverse effects, methods such as not using the sediment in places where problems are suspected, and not using the surface soil or surface dredged soil containing specific organisms that are problematic can be considered. However, it is difficult with conventional techniques to prove that the specific organisms that are problematic are not contained therein. Patent Document 1 is a method of directly extracting DNA from soil, but the sample amount is small for targeting plants contained in sediment. Patent Document 2 is a method of confirming distributed organisms by analyzing DNA in the environment. Environmental DNA analysis is mainly a technique targeting water and is not applicable to the above problems.

[0008] Since organisms in sediment are unevenly distributed in specific locations and DNA is considered to have low mobility in soil, it is not appropriate to determine the presence or absence of organisms in thousands to tens of thousands of cubic meters of sediment and judge the feasibility of sediment utilization based on the analysis results of a small amount of samples. To determine the presence or absence of target organisms in the entire large amount of sediment using a small amount of samples, sampling at multiple locations and analysis of a large amount of samples are required. However, as shown in Figure 4, even when analyzing a large number of samples collected from many locations P in the sediment SA to be used, portion TA shown by dashed line the situation of is not necessarily clear.

[0009] In view of the problems of the prior art as described above, the present invention provides a sediment analysis method by for evaluating sediment utilization and method of using earth and sand that can evaluate the utilization of sediment based on the presence or absence of target organisms and the like for the sediment to be used.

Means for Solving the Problems

[0010] A method for evaluating sediment utilization by a first sediment analysis method for achieving the above object is Preset a target organism that may be contained in the earth and sand to be used and that includes at least any one of alien organisms and organisms whose movement is to be suppressed. a step of charging the sediment to be evaluated into a storage section, a step of stirring the sediment and water in the storage section, and a step of collecting water from the storage section after stirring and analyzing the environmental DNA derived from the target organism for the collected water, in a sediment analysis method including wherein detecting the target organism based on the analysis result of the environmental DNA presence or absence of or the biomass of the target organism and evaluating the feasibility of utilization for the sediment based on the detection.

[0011] According to the first sediment analysis method evaluation method of earth and sand utilization by the sediment to be used is charged into the storage section as the sediment to be evaluated, and the sediment in the storage section and water After stirring, water is sampled from the storage tank, and environmental DNA is analyzed for this water. Therefore, the DNA contained in the sediment to be evaluated can be surely eluted into the water by stirring the sediment, and then environmental DNA analysis can be performed on this water. The sediment to be used is the object of evaluation. Even if a large amount of sediment is the object of evaluation, it can be carried out in several portions as needed. By stirring in the storage tank, environmental DNA analysis of the whole sediment to be evaluated can be performed for the target organism. Thus, since environmental DNA analysis of the target organism is performed on the sediment to be used, the usability can be accurately judged.

[0012] Note that environmental DNA is DNA derived from organisms released into the environment such as in water, soil, and air. By collecting and analyzing such environmental DNA, the presence or absence and the amount of biomass of the target organism can be estimated.

[0013] The method for evaluating the use of sediment by the second sediment analysis method for achieving the above object is Preset a target organism that may be contained in the earth and sand to be used and that includes at least any one of alien organisms and organisms whose movement is to be suppressed. a step of introducing the sediment to be evaluated into a storage tank, a step of stirring the sediment and water in the storage tank, visually observing the floating matter floating on the water surface in the storage tank after the stirring, and the a step of determining whether the target organism is contained in the floating matter, sampling water from the storage tank, and the a step of analyzing environmental DNA derived from the target organism for the sampled water, in a sediment analysis method including wherein the detection of the target organism based on the visual observation result presence or absence of or the number of individuals of the target organism based on 、 and / or Based on the presence or absence of detection of the target organism or the biomass of the target organism according to the analysis result of the environmental DNA evaluating the usability of the sediment.

[0014] According to the second sediment analysis method evaluation method of earth and sand utilization by the sediment to be used is introduced into the storage tank as the sediment to be evaluated, and the sediment in the storage tank and water After stirring, visually observe the floating matter that has risen to the water surface inside the storage tank, and determine whether the target organisms are included in the floating matter. Thus, it is possible to surely cause the target organisms or parts of the target organisms contained in the sediment to be evaluated to float to the water surface by stirring the sediment and perform visual observation. Further, since water is collected from inside the storage tank after stirring and environmental DNA is analyzed for the water, it is possible to surely elute the DNA contained in the sediment to be evaluated into the water by stirring the sediment and then perform environmental DNA analysis on the water. The sediment to be used is the object of evaluation, and even if a large amount of sediment is the object of evaluation, it can be carried out in several portions as necessary. By stirring inside the storage tank, visual observation of the target organisms and analysis of environmental DNA derived from the target organisms can be performed on the entire sediment to be evaluated. In this way, since the analysis of the target organisms is performed on the sediment to be used, it is possible to accurately judge whether it can be used.

[0015] In the method for evaluating sediment utilization by the second sediment analysis method, when it is determined by the visual observation that the floating matter the does not contain the target organisms, or when the determination is impossible, DNA analysis of the floating matter may be performed.

[0016] The first and second sediment analysis methods evaluation method of earth and sand utilization by In this case, it is preferable that the sediment to be evaluated is the total amount of the sediment to be used. By making the entire sediment to be used the object of evaluation, it is possible to surely judge whether the entire sediment to be used can be used.

[0017] Also 、 when the sediment to be used is 10,000 m 3 or more, 1,000 m 3 of the sediment to be used per 3 may be taken as the object of evaluation. In this case, the entire sediment to be used is judged based on the results of the sediment to be evaluated.

[0018] It is preferable to add water to the storage section before and / or during the stirring. By adding water as needed, even when the water content of the earth and sand is low, elution of DNA into water in the storage section, water sampling from the storage section, and floating of the target organism to the water surface become possible.

[0019] The stirring is preferably performed using at least one of a mixer, an excavator, and pneumatic conveyance.

[0020] The storage section preferably has a volume corresponding to the volume of the earth and sand to be evaluated. As such a storage section, it is preferable to use a steel water tank, an excavated pond, or the earth tank of an earth hauler, and an appropriate storage section can be selected according to the volume of the earth and sand to be evaluated. Thereby, it is possible to evaluate a relatively large amount of earth and sand on the order of several tens to several thousand m 3 as the object.

[0021] For achieving the above object utilization of earth and sand the method is the first or second earth and sand analysis method evaluation method of earth and sand utilization by to For the earth and sand evaluated to be available for the above utilization, it is used in an area other than the original extraction location of the earth and sand. .

[0022] According to this utilization of earth and sand method, since the usability of the earth and sand is determined based on the detection result of the target organism by at least any one of environmental DNA analysis, DNA analysis, and observation of the earth and sand of the utilization target to be evaluated, it is possible to accurately and reliably determine and evaluate the usability of the earth and sand of the utilization target. The earth and sand evaluated to be available for utilization can be used in an area other than the original extraction location of the earth and sand. .

[0023] Another method for using earth and sand for achieving the above object is to exclude from the utilization target, or cement solidification, heat treatment, or fumigation treatment perform and then use the earth and sand evaluated as not usable by the evaluation method of earth and sand utilization by the first or second earth and sand analysis method.

[0024] According to this method of using earth and sand, When it is determined that the use of the sediment to be evaluated is not possible, the use of the sediment can be stopped, or appropriate treatment can be performed on the sediment and it can be used for limited purposes.

[0025] In addition, the target organisms to be detected in the method for evaluating sediment utilization by the first and second analysis methods are set in advance. ... Examples of such target organisms include alien organisms with restricted movement (including specific alien organisms designated by the Ministry of the Environment) and natural enemy organisms of fishery useful species.

Advantages of the Invention

[0026] According to the present invention, there is provided a method for analyzing sediment capable of evaluating the entire sediment to be used in terms of the presence or absence of target organisms. by Method for evaluating sediment utilization and method of using earth and sand can be provided.

Brief Description of the Drawings

[0027]

Figure 1

Figure 2

Figure 3

Figure 4

Embodiments for Carrying Out the Invention

[0028] Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings. FIG. 1 is a flowchart for explaining each step of the method for evaluating the use of earth and sand according to this embodiment. FIG. 2 is a side view of a storage tank section schematically showing each step of inputting, stirring, confirming floating matter, and water sampling of the earth and sand in FIG. 1.

[0029] Referring to FIGS. 1 and 2, first, the earth and sand SA to be evaluated is put into the storage tank section 11 (S01). The earth and sand SA may be dredged soil, muddy soil, or construction waste in addition to earth and sand, and is targeted for effective use and wide-area use. Since there is a possibility that target organisms such as unwanted alien organisms and microorganisms are included at the place where the earth and sand is used, it is the object of evaluation. The earth and sand to be evaluated is the total amount of the earth and sand to be used. Also, the input of the earth and sand to be evaluated may be carried out once and then the subsequent stirring process may be performed, or it may be input in several portions while stirring.

[0030] The storage tank section 11 is selected according to the volume of the earth and sand to be evaluated. When the volume of the earth and sand is several to 20 m 3 a steel water tank is used. When it is several tens to several hundreds of m 3 a dugout pond is used. When it is several hundreds to 2000 m 3 it is preferable to use the earth tank of an earth hauler. In addition, when the storage tank section cannot handle the earth and sand to be evaluated at one time, it is carried out in several portions.

[0031] Next, water is added to the storage tank 11 as needed (S02). This ensures that there is a sufficient amount of water 12 in the storage tank 11, creating a state where the target organism or a part of it can float and water can be sampled. The water addition is carried out before the next stirring step, but it may also be done during stirring or even before the sediment SA is introduced into the storage tank 11. By adding water as needed, even when the moisture content of the sediment is low, DNA can be eluted into the water in the storage tank 11, water can be sampled from the storage tank 11, and the target organism can float on the water surface within the storage tank 11. When using seawater as the water to be added, it is preferable to analyze and evaluate the environmental DNA of the seawater to be used in advance and then conduct the evaluation of the environmental DNA of the collected sample based on the evaluation of the seawater. Also, in cases where the water content is high, such as in dredged soil with a high water content ratio, the water addition step may be omitted.

[0032] Next, the sediment SA in the storage tank 11 is stirred (S03). By such stirring, the target organism or a part of it in the sediment can be floated in the storage tank 11, and the DNA contained in the sediment can be reliably eluted into the water 12. For example, as shown in Fig. 2, the sediment SA in the storage tank 11 is stirred by operating the bucket BK attached to the tip of the arm with the backhoe BH. It is preferable to use a mixing bucket, a skeleton bucket, or the like as the bucket BK. Also, instead of the backhoe, a mixer, pneumatic conveying (in-pipe mixing by plug flow), or the like may be used for stirring.

[0033] It is possible to increase the floating of the target organism and the extraction efficiency of environmental DNA by using a stirring method that breaks the organisms in the sediment during stirring and promotes the elution of DNA in the individuals. Such stirring methods include, for example, crushing the organisms by contact with the sediment during mixer or pneumatic conveying, and cutting the organisms by sharpening the blades of the mixing bucket.

[0034] Next, visually observe the floating objects 13 such as biological individuals and fragments floating on the water surface of the water 12 in the storage tank section 11, and determine whether the target organism is included (S04). As such floating objects 13, for example, plant bodies and plant seeds in the earth and sand, insects (arthropods), individuals that died due to the stirring operation, etc. may float.

[0035] If the target organism preset in the visual observation step S04 is not detected (absence in S05), next, water is sampled from the water 12 in the storage tank section 11 (S06). Such water sampling is preferably performed after standing for a predetermined time as necessary after stirring. The amount of water sampled is preferably about 1,000 to 5,000 mL, for example. In the case of a small water storage section, water sampling from one location may be sufficient, but when using the earth tank of an earth transport ship, which is a large storage tank section, it is desirable to sample from at least three locations.

[0036] Also, if the target organism preset in the visual observation step S04 is not detected (absence in S05), the floating object 13 floating on the water surface is collected (S07).

[0037] Next, environmental DNA analysis is performed on the water sampled as described above (S08). By amplifying and analyzing the DNA derived from the target organism contained in the sampled water through such environmental DNA analysis, it is possible to determine the presence or absence, type, and approximate biomass of the target organism in the earth and sand SA to be evaluated. In addition, by comprehensively amplifying and analyzing the DNA contained in the sampled water, it is also possible to list up the species of organisms that may exist in the earth and sand SA to be evaluated, which could not be confirmed by visual observation. Also, the environmental DNA analysis is performed in accordance with the "Environmental DNA Survey and Experiment Manual" by the Environmental DNA Society (see Non-Patent Document 1).

[0038] Also, DNA analysis is performed on the floating matter 13 that has floated to the water surface and been collected in step S07 (S09). For floating matter such as plant leaves, stems, roots, and parts of insect bodies, when it is difficult or impossible to distinguish the species by visual observation, DNA analysis of the floating matter can surely determine whether the floating matter is the target organism. Such DNA analysis is easier to detect the target organism because the concentration is higher than that eluted in water. Note that the floating matter collection step S07 and the DNA analysis step S09 may be performed as necessary, and may be omitted when the floating matter can be identified by visual observation.

[0039] If the target organism preset in the environmental DNA analysis step S08 and the DNA analysis step S09 is not detected (absence in S10), the earth and sand SA to be evaluated is used as normal earth and sand, dredged soil, or construction waste soil (S11), enabling the effective utilization and wide-area utilization of the earth and sand SA.

[0040] Also, if the target organism preset in the visual observation step S05, the environmental DNA analysis step S08, or the DNA analysis step S09 is detected (presence in S05, presence in S10), the earth and sand SA to be evaluated is excluded from the usage target, or, if necessary, measures such as cement solidification, the soil treatment method proposed by the applicant in Japanese Patent Application No. 2019-202962, heat treatment, and fumigation treatment are implemented and then used (S12). Note that the earth and sand after these measures may have restricted uses.

[0041] According to the method for evaluating the utilization of earth and sand including the method for analyzing earth and sand according to this embodiment, the total amount of the earth and sand to be used is put into the storage section 11 as the earth and sand SA to be evaluated, the earth and sand SA in the storage section 11 is stirred, and then the floating matter 13 floating on the water surface in the storage section 11 is visually observed to check whether the target organism is included in the floating matter 13. Therefore, the target organism or a part thereof included in the earth and sand SA to be evaluated can be floated to the water surface by stirring the earth and sand and the target organism can be confirmed by visual observation. The total amount of the earth and sand to be used is the evaluation target, and even if a large amount of earth and sand is the evaluation target, the target organism can be confirmed for the entire earth and sand by stirring in the storage section 11 in multiple portions as necessary.

[0042] In addition, since water 12 is sampled as a sample from within the storage tank portion 11 and environmental DNA analysis is performed on the water, the DNA contained in the sediment to be evaluated can be eluted into the water by stirring the sediment SA, and then environmental DNA analysis can be performed on the water. The entire amount of the sediment to be used is the evaluation target. Even when a large amount of sediment is the evaluation target, if necessary, the sediment SA can be stirred in the storage tank portion 11 in multiple portions to transfer the DNA into the water, and since the water is easily homogenized, environmental DNA analysis of the entire sediment is possible.

[0043] As described above, since visual observation and / or environmental DNA analysis can be performed on the entire sediment to be used to determine the presence or absence of target organisms, etc., it is possible to accurately and reliably determine and evaluate whether the target sediment can be used.

[0044] Also, by adding water to the sediment to be evaluated and stirring it, eluting the DNA in the sediment into the water, and transferring the target organisms into the water is a large-scale extraction operation. After such an extraction operation, visual observation of the floating matter floating on the water surface and environmental DNA analysis by collecting water samples, etc., are performed, so it is possible to surely determine and evaluate whether the sediment to be evaluated can be used. Also, since the water is easily homogenized, for example, even for sediment with a large capacity of about 2000 m 3 the entire sediment can be evaluated by environmental DNA analysis.

[0045] In this embodiment, based on the detection result of the floating matter by visual observation, the presence or absence of specific alien organisms and species of organisms whose movement is desired to be suppressed is confirmed as the detection target organisms. When the target species is not confirmed in the floating matter, environmental DNA analysis of the sampled water sample and DNA analysis of the floating matter are performed as necessary to confirm plants, fish, shellfish, benthic organisms, arthropods, etc. contained in the sediment, and the presence or absence of the target organisms can be confirmed. Also, when necessary by environmental DNA analysis or DNA analysis of floating matter microorganisms can also be targeted.

[0046] When checking the occurrence status of a specific biological species, it is also possible to make a judgment on-site in about 30 minutes by using mobile real-time PCR with a real-time PCR device or the like.

[0047] The above analysis methods (floatation separation and visual confirmation after water addition, environmental DNA analysis, DNA analysis of floating substances) according to the present embodiment can be used for preliminary determination and selection (screening) of soil and dredged soil, and management during the use of soil and dredged soil (environmental DNA analysis once every several thousand m 3 For example, when 10,000 m 3 of soil is the object of use, and it is put into the soil tank of a soil transport ship, water is added, and stirring is carried out in units of 1000 m 3 in the case of whole quantity evaluation, 10 ship loads are the objects of evaluation. For example, environmental DNA analysis is performed by collecting 3 samples / soil transport ship.

[0048] In addition, it is preferable to preset alien organisms (including specific alien organisms) and organisms whose movement is to be suppressed as the organisms to be detected. Alien organisms refer to alien species of overseas origin, and specific alien organisms are designated by the Ministry of the Environment from among alien organisms that cause or may cause damage to ecosystems, human life and health, and agriculture, forestry and fisheries. It is not limited to living things, and includes eggs, seeds, organs, etc. as well as individuals. As of November 2020, 25 species of insects such as termites and Argentine ants, 7 species of spiders and scorpions such as Nephila clavata and Argiope bruennichi, 5 species of mollusks, etc., and 19 species of plants such as Alecthrium (see Non-Patent Document 2) are the main organisms to be detected in the case of soil and dredged soil.

[0049] The third of the three principles for preventing damage caused by alien species by the Ministry of the Environment for preventing damage caused by alien organisms is not to "spread" (including not increasing) alien species that already exist in the wild to other regions. The highly venomous Sydney funnel-web spider native to Australia has already bred in western Japan, and as some have been carried in vehicles and discovered even in far-off Hokkaido, it is important not to spread it any further from its current habitats (see Non-Patent Document 3). However, according to the method for evaluating the use of earth and sand in this embodiment, it is possible to prevent the movement and spread of alien organisms that occur when effectively using or widely using earth and sand.

[0050] In addition, the stirring time in the stirring step S03 of FIG. 1 is preferably about 15 minutes to 60 minutes, for example. However, it is preferable to determine the stirring time by a preliminary test for each amount of soil to be stirred and stirring method. For example, it is preferable to change the stirring time of the earth and sand, analyze the environmental DNA, and set the time when the same number of species appearing and the environmental DNA concentration of the target organisms reach a certain value.

[0051] That is, to confirm the influence of the stirring time, when dredged soil is put into the soil tank of a dump truck, if necessary, after adding water to the dredged soil, water is sampled multiple times at intervals of, for example, 5 to 10 minutes before and after stirring with an excavator, and the environmental DNA (fish, shellfish, benthic organisms, etc.) of the water sampling sample and the sediment is analyzed. Also, when earth and sand is put into a steel water tank, after putting earth and sand into the steel water tank and adding water, water is sampled multiple times at intervals of, for example, 5 to 10 minutes before and after stirring with a mixing bucket, and the environmental DNA (plants, insects, etc.) of the water sampling sample and the earth and sand sample is analyzed. It is preferable to set the stirring time based on these analysis results.

[0052] (Experimental Example) An example of an environmental DNA analysis experiment conducted in a laboratory test will be described. The experimental results of this example are shown in Fig. 3. As shown in the figure, after adding 1 g of seeds of a plant (Cucurbita ficifolia (Cucurbitaceae)) to 1 kg of soil and mixing them, tap water was added and stirred 10 times with a hand scoop. After standing for 1 hour, 1 liter of water sample was collected for DNA analysis, and as a result, Cucurbita ficifolia was detected. On the other hand, as a result of collecting a small amount of soil sample of about 500 mg from the soil before adding water and analyzing the DNA, Cucurbita ficifolia was not detected. Although Miscanthus transmorrisonensis was detected from the above soil sample and water sample, Miscanthus transmorrisonensis is a plant widely distributed around the collection location of the soil used, and such species can be detected even when a small amount of soil sample is collected and analyzed. In contrast, the added small amount of Cucurbita ficifolia seeds were not present in the original soil and were unevenly distributed in the soil, so they were not detected from a small amount of soil sample, but were detected in the water sample after adding water and stirring.

[0053] As described above, the embodiments for carrying out the present invention have been explained, but the present invention is not limited to these, and various modifications are possible within the scope of the technical idea of the present invention. For example, in Fig. 1, when the target organism is not detected by the visual observation S04 of the floating matter (absence of S05), environmental DNA analysis and DNA analysis of the floating matter are performed. However, the present invention is not limited to this, and environmental DNA analysis may be performed regardless of the presence or absence of detection in step S05, and further, DNA analysis of the floating matter may be performed as necessary. Thereby, for example, relatively large organisms can be confirmed relatively easily by visual observation, but it is possible to cope with cases where it is relatively difficult or impossible to confirm by visual observation, such as fragments of individuals, plants, and plant seeds, or cases where microorganisms are included as the target organism to be detected.

[0054] Also, in this embodiment, the usability of the target soil was evaluated based on the presence or absence of detection of the target organism, but the present invention is not limited to this, and the usability of the target soil may be evaluated based on the biomass or the number of individuals of the target organism.

[0055] Also, a chemical agent such as skim milk may be added to the soil or dredged soil and stirred to increase the extraction efficiency of environmental DNA.

[0056] In addition, in this embodiment, the entire earth and sand to be used is taken as the evaluation target. However, the present invention is not limited to this. When an extremely large amount of earth and sand is the use target and a part of the large amount of earth and sand is taken as the evaluation target, it is also preferably applicable. When the earth and sand to be used is 10,000 m 3 or more, 1000 m 3 of the earth and sand per 10,000 m 3 of the earth and sand to be used may be taken as the evaluation target. In this case, the entire earth and sand to be used is judged based on the result of the earth and sand to be evaluated. Also, for example, when the earth and sand to be used is 10,000 m 3 or more, at least 1000 m 3 of the earth and sand is used as a representative sample and is also applicable when it is taken as the evaluation target. According to the present invention, it is possible to evaluate a large amount of earth and sand at the 1000 m 3 level. This method is suitable, for example, when removing the surface layer where organisms are distributed even if there are alien organisms and using the subsoil, so the possibility of biological mixing is small, when it is carried out for confirmation, or when dealing with a large amount of earth and sand in land reclamation etc. where the possibility of the appearance of the target organism is low. On the other hand, when the importance is high due to the possibility of the existence of specific alien organisms etc., it is possible to evaluate the entire earth and sand to be used by taking the total amount of the earth and sand to be used as the evaluation target.

[0057] In addition, as is clear from the description of the above-described method for evaluating the use of earth and sand, the present invention is also established as a method for using earth and sand. Such a method for using earth and sand is to use the earth and sand evaluated as being usable by the above-described method for evaluating the use of earth and sand in an area other than the original extraction location of the earth and sand.

Industrial Applicability

[0058] According to the present invention, since the evaluation of the use of the earth and sand to be used is carried out based on the presence or absence of target organisms such as alien organisms whose movement is restricted, when effectively using or widely using earth and sand, construction-generated earth, dredged soil, etc., the movement and spread of the target organisms can be prevented in advance.

Explanation of Signs

[0059] 11 Storage part 12 Water in storage part 11 13 Floating matter Sediment to be evaluated by SA BK Bucket BH Backhoe

Claims

1. An evaluation method for earthwork utilization by an earthwork analysis method with the earthwork to be utilized as the evaluation target, wherein a target organism that may be contained in the earthwork to be utilized and includes at least one of alien organisms and organisms whose movement is to be suppressed is preset in advance, a step of charging the earthwork to be evaluated into a storage section, a step of stirring the earthwork and water in the storage section, a step of collecting water from the storage section after the stirring and analyzing environmental DNA derived from the target organism in the collected water, in the earthwork analysis method including: an evaluation method for earthwork utilization that evaluates the usability of the earthwork based on the presence or absence of detection of the target organism or the biomass of the target organism according to the analysis result of the environmental DNA.

2. An evaluation method for earthwork utilization by an earthwork analysis method with the earthwork to be utilized as the evaluation target, wherein a target organism that may be contained in the earthwork to be utilized and includes at least one of alien organisms and organisms whose movement is to be suppressed is preset in advance, a step of charging the earthwork to be evaluated into a storage section, a step of stirring the earthwork and water in the storage section, a step of visually observing the floating matter floating on the water surface in the storage section after the stirring and determining whether the target organism is contained in the floating matter, a step of collecting water from the storage section and analyzing environmental DNA derived from the target organism in the collected water, in the earthwork analysis method including: an evaluation method for earthwork utilization that evaluates the usability of the earthwork based on the presence or absence of detection of the target organism or the number of individuals of the target organism according to the visual observation result and / or based on the presence or absence of detection of the target organism or the biomass of the target organism according to the analysis result of the environmental DNA.

3. The evaluation method for earthwork utilization according to claim 2, wherein when it is determined that the target organism is not contained in the floating matter by the visual observation, or when the determination is impossible, DNA analysis of the floating matter is performed.

4. The evaluation method for earthwork utilization according to any one of claims 1 to 3, wherein the earthwork to be evaluated is the total amount of the earthwork to be utilized.

5. When the earth and sand to be used are 10,000 m ３ or more, for every 10,000 m of the earth and sand to be used ３ 1,000 m ３ of the earth and sand is taken as the object to be evaluated, and the method for evaluating the utilization of earth and sand according to any one of claims 1 to 3.

6. The evaluation method for earthwork utilization according to any one of claims 1 to 5, wherein water is added to the storage section before and / or during the stirring.

7. The evaluation method for utilization of earth and sand according to any one of claims 1 to 6, wherein the stirring is performed using at least one of a mixer, an excavator, and pneumatic transportation.

8. The evaluation method for utilization of earth and sand according to any one of claims 1 to 7, wherein the storage section has a volume corresponding to the volume of the earth and sand to be evaluated.

9. The evaluation method for utilization of earth and sand according to any one of claims 1 to 8, wherein a steel water tank, an excavated pond, or an earth tank of an earth hauler is used as the storage section.

10. A method for utilizing earth and sand, which utilizes the earth and sand evaluated as usable by the evaluation method for utilization of earth and sand according to any one of claims 1 to 9 in an area other than the original extraction location of the earth and sand.

11. A method for utilizing earth and sand, which excludes the earth and sand evaluated as unusable by the evaluation method for utilization of earth and sand according to any one of claims 1 to 9 from the objects of utilization, or utilizes the earth and sand after performing cement solidification, heat treatment, or fumigation treatment.

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