Esophageal cancer detection kit or device and detection method

Abstract

To provide a kit or a device for the detection of esophageal cancer and a method for detecting esophageal cancer.SOLUTION: The present invention provides a kit or a device for the detection of esophageal cancer, comprising nucleic acid(s) capable of specifically binding to miRNA(s) in a sample of a subject, and a method for detecting esophageal cancer, comprising measuring the miRNA in vitro.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a kit or device for detecting esophageal cancer, which contains a nucleic acid capable of specifically binding to a specific miRNA and is used for detecting the presence or absence of esophageal cancer in a subject, and a method for detecting esophageal cancer, which includes measuring the expression level of the miRNA using the nucleic acid.

Background Art

[0002] The esophagus is a tubular organ that transports food from the mouth to the stomach and is located between the trachea and the spine. The wall of the esophagus is divided into four layers from the inside to the outside: the mucosa, submucosa, muscularis propria, and adventitia, each of which is responsible for the function of transporting food from the mouth to the stomach (Non-Patent Document 1). According to the statistics of cancers by site in Japan in 2012 disclosed by the Cancer Countermeasure Information Center of the National Cancer Center, National Institute of Biomedical Innovation, the number of deaths due to esophageal cancer was 11,592, ranking 10th among cancer deaths by site. In addition, the risk of death due to esophageal cancer in Japan is 5.6 times higher in men than in women, and smoking and alcohol consumption are regarded as risk factors for esophageal cancer (Non-Patent Document 1). In the United States, it is said that esophageal cancer develops in 1 out of 125 men and 1 out of 435 women, and the estimated number of patients with esophageal cancer in 2014 is as high as 18,170, and about 15,450 of them are said to die (Non-Patent Document 1 )

[0003] The progression of esophageal cancer is defined in Non-Patent Document 2, and the stage is determined by the size of the tumor (Tis, T1-3 and T4a-b), lymph node metastasis (N1-3), distant metastasis (M0-1), etc. ​​​​​​Stage 0 (Tis / N0 / M0), stage IA (T1 / N0 / M0), stage IB (T 2 / N0 / M0), stage IIA (T3 / N0 / M0), stage IIB (T1-2 / N1 / M0), stage IIIA (T4a / N0 / M0, T3 / N1 / M0, and T1 -2 / N2 / M0), stage IIIB (T3 / N2 / M0), stage IIIC (T4 a / N1-2 / M0, T4b / M0, and N3 / M0), and stage IV (M1) are classified into.

[0004] The 5-year relative survival rate of esophageal cancer greatly depends on the progression of the cancer, and is 39% for cancer limited to the esophageal tissue, 21% for cancer remaining in adjacent tissues, and 4% for cancer with distant metastasis, as reported (Non-Patent Document 1). Therefore, early detection of esophageal cancer leads to a significant improvement in the survival rate, and thus there is a strong demand for providing means enabling early detection.

[0005] The treatment method of esophageal cancer is determined based on the progression of the cancer and the general condition of the body, and mainly includes endoscopic treatment, surgery, radiation therapy, and anticancer agents. For cancers that have progressed to a certain extent, a multidisciplinary treatment is performed to produce a synergistic effect while taking advantage of the characteristics of each treatment (Non-Patent Document 1). In addition, for early esophageal cancers such as stage 0 or 1, endoscopic treatment with less burden on the patient or photodynamic therapy may be applicable (Non-Patent Document 1).

[0006] According to Non-Patent Document 1, general examinations for esophageal cancer are X-ray esophagography and endoscopic examination. In addition, CT examination, MRI examination, endoscopic ultrasonography examination, ultrasonic examination, etc. are performed to check the spread of the cancer. When findings suggesting esophageal cancer are detected in these primary examinations, As a secondary examination, a pathological examination is performed in which a needle is inserted into the lesion to collect cells and tissues and examined under a microscope. In addition, as blood tumor markers for detecting esophageal cancer, for example, CEA, SCC, etc. are known (Non-Patent Document 3).

[0007] Although still in the research stage, as shown in Patent Document 1, there is a report that esophageal cancer can be detected by combining the expression level of microRNA (miRNA) in biological samples including blood with the expression level of other protein markers. .

[0008] Patent Document 1 shows a method for detecting esophageal cancer by measuring miRNAs such as miR-663a, miR-92a-3p, miR-575 in serum.

Prior Art Documents

Patent Documents

[0009]

Patent Document 1

Non-Patent Documents

[0010]

Non-Patent Document 1

Non-Patent Document 2

Non-Patent Document 3

[0011] The object of the present invention is to discover a novel esophageal cancer tumor marker and to develop a compound that specifically binds to the marker. The object of the present invention is to provide a method for effectively detecting esophageal cancer using nucleic acids.

[0012] As described in Non-Patent Document 1, the general examination for esophageal cancer is esophagography using X-rays. However, regular checkups focus on stomach cancer testing, and The roads are often not adequately observed. However, the number of deaths from esophageal cancer in Japan is steadily increasing, and imaging diagnosis is not necessarily the best treatment for esophageal cancer. It cannot be said that cancer is being suppressed. Also, CT scans and MRI scans are highly sophisticated and can only detect esophageal cancer. Although it is possible to detect the Therefore, it is not suitable for widespread use as a primary test.

[0013] Blood tumor markers for detecting esophageal cancer include CEA and SCC. However, these markers are also elevated in cancers other than esophageal cancer (Non-Patent Document 3), and Due to the toxicity issues, their usefulness has not been established. Therefore, if other cancers are misdiagnosed as esophageal cancer, opportunities for appropriate treatment may be missed or the wrong medical treatment may be given. This would impose unnecessary financial and physical burdens on patients. , these markers are not described in the American Cancer Society's esophageal cancer guidelines (Non-Patent Document 1).

[0014] Also, although still in the research stage, there are reports of using the expression levels of microRNAs (miR NA) in biological samples such as blood to discriminate esophageal cancer, but none of them has reached practical application.

[0015] Patent Document 1 shows a method of measuring miRNAs such as miR-663a, miR-92a-3p, and miR-575 in serum to detect esophageal cancer. Specifically, a list of miRNAs that vary in the serum of 16 esophageal cancer patients compared to 12 healthy individuals is shown , and the expression levels of those miRNAs are measured to discriminate whether it is esophageal cancer. However, this detection method has few examples and descriptions regarding the detection performance such as the specific accuracy, sensitivity, and specificity for discriminating esophageal cancer, and has poor industrial applicability. The only verified hsa-miR- 345 has an AUC of 0.814, and there are also descriptions indicating that it is difficult to discriminate esophageal cancer alone.

[0016] Thus, in the detection of esophageal cancer, existing tumor markers have low performance, or for markers in the research stage, the performance and detection methods are not specifically shown. Therefore, when using these , there may be unnecessary additional examinations due to misdetecting healthy individuals as esophageal cancer patients, or the loss of treatment opportunities due to overlooking esophageal cancer patients. Also, measuring dozens to hundreds of miRNAs increases the examination cost, so it is difficult to use in large-scale screenings such as health checkups. Furthermore, measuring tumor markers ​​Since collecting esophageal tissue is highly invasive and unfavorable to the patient, detection from minimally invasive collectable blood is possible, and there is a need for a highly accurate esophageal cancer marker that can correctly distinguish esophageal cancer patients as esophageal cancer patients and healthy individuals as healthy individuals. In particular, early detection and treatment of esophageal cancer can significantly improve the survival rate, and also, endoscopic treatment and photodynamic therapy, which impose less burden on the patient, can be applied in treatment selection. Therefore, there is an urgent need for an esophageal cancer marker with high sensitivity that can detect even early-stage esophageal cancer.

Means for Solving the Problems

[0017] As a result of intensive studies to solve the above problems, the present inventors have found several genes that can be used as detection markers for esophageal cancer in blood, and by using nucleic acids that can specifically bind thereto, it has been found that esophageal cancer can be significantly detected, leading to the completion of the present invention. <Summary of the Invention> That is, the present invention has the following features. (1) miR-204-3p, miR-1247-3p, mi R-6875-5p, miR-6857-5p, miR-6726-5p, miR-31 88, miR-8069, miR-4257, miR-1343-3p, miR-710 8-5p, miR-6825-5p, miR-7641, miR-3185, miR-4 746-3p, miR-6791-5p, miR-6893-5p, miR-4433b -3p, miR-3135b, miR-6781-5p, miR-1908-5p, mi R-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150 -3p, miR-4433-3p, miR-6125, miR-4513, miR-67 87-5p, miR-6784-5p, miR-615-5p, miR-6765-3p , miR-5572, miR-6842-5p, miR-8063, miR-6780b -5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR- 7975, miR-1233-5p, miR-6845-5p, miR-3937, mi R-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-807 2, miR-4516, miR-6756-5p, miR-4665-3p, miR-6 826-5p, miR-6820-5p, miR-6887-5p, miR-3679- 5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-684 8-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p , miR-3620-5p, miR-3131, miR-6732-5p, miR-71 13-3p, miR-23a-3p, miR-3154, miR-4723-5p, mi R-3663-3p, miR-4734, miR-6816-5p, miR-4442, miR-4476, miR-423-5p, miR-1249, miR-6515-3p , miR-887-3p, miR-4741, miR-6766-3p, miR-467 3, miR-6779-5p, miR-4706, miR-1268b, miR-463 2-5p, miR-3197, miR-6798-5p, miR-711, miR-68 40-3p, miR-6763-5p, miR-6727-5p, miR-371a-5 p, miR-6824-5p, miR-4648, miR-1227-5p, miR-5 64, miR-3679-3p, miR-2861, miR-6737-5p, miR- 4725-3p, miR-6716-5p, miR-4675, miR-1915-3p , miR-671-5p, miR-3656, miR-6722-3p, miR-470 7-5p, miR-4449, miR-1202, miR-4649-5p, miR-7 44-5p, miR-642a-3p, miR-451a, miR-6870-5p, m iR-4443, miR-6808-5p, miR-4728-5p, miR-937- 5p, miR-135a-3p, miR-663b, miR-1343-5p, miR- 6822-5p, miR-6803-5p, miR-6805-3p, miR-128- 2-5p, miR-4640-5p, miR-1469, miR-92a-2-5p, m iR-3940-5p, miR-4281, miR-1260b, miR-4758-5 p, miR-1915-5p, miR-5001-5p, miR-4286, miR-6 126, miR-6789-5p, miR-4459, miR-1268a, miR-6 752-5p, miR-6131, miR-6800-5p, miR-4532, miR -6872-3p, miR-718, miR-6769a-5p, miR-4707-3 p, miR-6765-5p, miR-4739, miR-4525, miR-4270 , miR-4534, miR-6785-5p, miR-6850-5p, miR-46 97-5p, miR-1260a, miR-4486, miR-6880-5p, miR -6802-5p, miR-6861-5p, miR-92b-5p, miR-1238 -5p, miR-6851-5p, miR-7704, miR-149-3p, miR- 4689, miR-4688, miR-125a-3p, miR-23b-3p, miR -614, miR-1913, miR-16-5p, miR-6717-5p, miR- 3648, miR-3162-5p, miR-1909-3p, miR-8073, mi R-6769b-5p, miR-6836-3p, miR-4484, miR-6819 -5p and at least one polynucleotide selected from the group consisting of miR-6794-5p that can specifically bind to a nucleic acid A kit for detecting esophageal cancer, comprising a nucleic acid that can specifically bind to a polynucleotide (2) miR-204-3p is hsa-miR-204-3p, and miR-1247 -3p is hsa-miR-1247-3p, and miR-6875-5p is hsa-m iR-6875-5p, and miR-6857-5p is hsa-miR-6857-5 p, and miR-6726-5p is hsa-miR-6726-5p, and miR- 3188 is hsa-miR-3188, and miR-8069 is hsa-miR-80 69, and miR-4257 is hsa-miR-4257, and miR-1343- 3p is hsa-miR-1343-3p, and miR-7108-5p is hsa-mi It is R-7108-5p, and miR-6825-5p is hsa-miR-6825-5p It is miR-7641 is hsa-miR-7641, and miR-3185 is hs a-miR-3185, and miR-4746-3p is hsa-miR-4746-3 p, miR-6791-5p is hsa-miR-6791-5p, and miR- 6893-5p is hsa-miR-6893-5p, and miR-4433b-3p is hsa-miR-4433b-3p, miR-3135b is hsa-miR-31 35b, miR-6781-5p is hsa-miR-6781-5p, and mi R-1908-5p is hsa-miR-1908-5p, and miR-4792 is hs a-miR-4792, miR-7845-5p is hsa-miR-7845-5 p, miR-4417 is hsa-miR-4417, and miR-3184-5 p is hsa-miR-3184-5p, miR-1225-5p is hsa-miR -1225-5p, miR-1231 is hsa-miR-1231, and miR -1225-3p is hsa-miR-1225-3p, miR-150-3p is h sa-miR-150-3p, miR-4433-3p is hsa-miR-443 3-3p, miR-6125 is hsa-miR-6125, and miR-451 3 is hsa-miR-4513, miR-6787-5p is hsa-miR-67 87-5p, miR-6784-5p is hsa-miR-6784-5p, and miR-615-5p is hsa-miR-615-5p, miR-6765-3p is hsa-miR-6765-3p, miR-5572 is hsa-miR-557 is 2, miR-6842-5p is hsa-miR-6842-5p, miR- 8063 is hsa-miR-8063, miR-6780b-5p is hsa-mi R-6780b-5p, miR-187-5p is hsa-miR-187-5p, and miR-128-1-5p is hsa-miR-128-1-5p, miR- 6729-5p is hsa-miR-6729-5p, miR-6741-5p is h sa-miR-6741-5p, miR-6757-5p is hsa-miR-67 57-5p, miR-7110-5p is hsa-miR-7110-5p, miR-7975 is hsa-miR-7975, miR-1233-5p is hsa -miR-1233-5p, miR-6845-5p is hsa-miR-6845 -5p, miR-3937 is hsa-miR-3937, miR-4467 is hsa-miR-4467, miR-7109-5p is hsa-miR-710 9-5p, miR-6088 is hsa-miR-6088, miR-678 2-5p is hsa-miR-6782-5p, miR-5195-3p is hsa- miR-5195-3p, miR-4454 is hsa-miR-4454, miR-6724-5p is hsa-miR-6724-5p, miR-8072 is hsa-miR-8072, miR-4516 is hsa-miR-4516, and miR-6756-5p is hsa-miR-6756-5p, miR-4665 -3p is hsa-miR-4665-3p, miR-6826-5p is hsa-m It is iR-6826-5p, and miR-6820-5p is hsa-miR-6820-5 p, miR-6887-5p is hsa-miR-6887-5p, miR- 3679-5p is hsa-miR-3679-5p, miR-7847-3p is h sa-miR-7847-3p, miR-6721-5p is hsa-miR-67 21-5p, miR-3622a-5p is hsa-miR-3622a-5p and miR-939-5p is hsa-miR-939-5p, miR-602 is h sa-miR-602, miR-7977 is hsa-miR-7977, m iR-6749-5p is hsa-miR-6749-5p, miR-1914-3 p is hsa-miR-1914-3p, miR-4651 is hsa-miR-46 51, miR-4695-5p is hsa-miR-4695-5p, miR -6848-5p is hsa-miR-6848-5p, miR-1228-3p is hsa-miR-1228-3p, miR-642b-3p is hsa-miR-6 42b-3p, miR-6746-5p is hsa-miR-6746-5p and miR-3620-5p is hsa-miR-3620-5p, miR-3131 is hsa-miR-3131, miR-6732-5p is hsa-miR-673 2-5p, miR-7113-3p is hsa-miR-7113-3p, m iR-23a-3p is hsa-miR-23a-3p, miR-3154 is hsa -miR-3154, miR-4723-5p is hsa-miR-4723-5p and miR-3663-3p is hsa-miR-3663-3p, miR-4 734 is hsa-miR-4734, miR-6816-5p is hsa-miR- 6816-5p, miR-4442 is hsa-miR-4442, miR- 4476 is hsa-miR-4476, miR-423-5p is hsa-miR- 423-5p, miR-1249 is hsa-miR-1249, miR-6 515-3p is hsa-miR-6515-3p, miR-887-3p is hsa -miR-887-3p, miR-4741 is hsa-miR-4741, miR-6766-3p is hsa-miR-6766-3p, miR-4673 is hsa-miR-4673, miR-6779-5p is hsa-miR-6779 -5p, miR-4706 is hsa-miR-4706, miR-1268 b is hsa-miR-1268b, miR-4632-5p is hsa-miR-4 632-5p, miR-3197 is hsa-miR-3197, miR-6 798-5p is hsa-miR-6798-5p, miR-711 is hsa-mi R-711, miR-6840-3p is hsa-miR-6840-3p, miR-6763-5p is hsa-miR-6763-5p, miR-6727- 5p is hsa-miR-6727-5p, miR-371a-5p is hsa-mi R-371a-5p, miR-6824-5p is hsa-miR-6824-5p , miR-4648 is hsa-miR-4648, miR-1227-5p is hsa-miR-1227-5p, miR-564 is hsa-miR-564 Yes, miR-3679-3p is hsa-miR-3679-3p, and miR-28 61 is hsa-miR-2861, and miR-6737-5p is hsa-miR-6 737-5p, and miR-4725-3p is hsa-miR-4725-3p , miR-6716-5p is hsa-miR-6716-5p, and miR-4675 is hsa-miR-4675, and miR-1915-3p is hsa-miR-191 5-3p, and miR-671-5p is hsa-miR-671-5p, miR -3656 is hsa-miR-3656, and miR-6722-3p is hsa-mi R-6722-3p, and miR-4707-5p is hsa-miR-4707-5p , miR-4449 is hsa-miR-4449, and miR-1202 is hs a-miR-1202, and miR-4649-5p is hsa-miR-4649-5 p, and miR-744-5p is hsa-miR-744-5p, miR-64 2a-3p is hsa-miR-642a-3p, and miR-451a is hsa-mi R-451a, and miR-6870-5p is hsa-miR-6870-5p , miR-4443 is hsa-miR-4443, and miR-6808-5p is hs a-miR-6808-5p, and miR-4728-5p is hsa-miR-472 8-5p, and miR-937-5p is hsa-miR-937-5p, miR -135a-3p is hsa-miR-135a-3p, and miR-663b is hsa -miR-663b, and miR-1343-5p is hsa-miR-1343-5p is hsa-miR-6822-5p, and miR-6 803-5p is hsa-miR-6803-5p, and miR-6805-3p is hs a-miR-6805-3p, and miR-128-2-5p is hsa-miR-12 8-2-5p, and miR-4640-5p is hsa-miR-4640-5p , miR-1469 is hsa-miR-1469, and miR-92a-2-5p is h sa-miR-92a-2-5p, and miR-3940-5p is hsa-miR-3 940-5p, and miR-4281 is hsa-miR-4281, miR-1 260b is hsa-miR-1260b, and miR-4758-5p is hsa-mi R-4758-5p, and miR-1915-5p is hsa-miR-1915-5p , and miR-5001-5p is hsa-miR-5001-5p, miR-4 286 is hsa-miR-4286, and miR-6126 is hsa-miR-612 6, and miR-6789-5p is hsa-miR-6789-5p, miR- 4459 is hsa-miR-4459, and miR-1268a is hsa-miR-1 268a, and miR-6752-5p is hsa-miR-6752-5p, m iR-6131 is hsa-miR-6131, and miR-6800-5p is hsa- miR-6800-5p, and miR-4532 is hsa-miR-4532, miR-6872-3p is hsa-miR-6872-3p, and miR-718 is h sa-miR-718, and miR-6769a-5p is hsa-miR-6769a -5p, and miR-4707-3p is hsa-miR-4707-3p, mi R-6765-5p is hsa-miR-6765-5p, and miR-4739 is hs a-miR-4739, and miR-4525 is hsa-miR-4525, m iR-4270 is hsa-miR-4270, and miR-4534 is hsa-miR -4534, and miR-6785-5p is hsa-miR-6785-5p, and miR-6850-5p is hsa-miR-6850-5p, miR-4697- 5p is hsa-miR-4697-5p, and miR-1260a is hsa-miR- 1260a, and miR-4486 is hsa-miR-4486, miR-68 80-5p is hsa-miR-6880-5p, and miR-6802-5p is hsa -miR-6802-5p, and miR-6861-5p is hsa-miR-6861 -5p, and miR-92b-5p is hsa-miR-92b-5p, miR- 1238-5p is hsa-miR-1238-5p, and miR-6851-5p is h sa-miR-6851-5p, and miR-7704 is hsa-miR-7704 and miR-149-3p is hsa-miR-149-3p, miR-4689 is hsa-miR-4689, and miR-4688 is hsa-miR-4688 and miR-125a-3p is hsa-miR-125a-3p, miR-23b -3p is hsa-miR-23b-3p, and miR-614 is hsa-miR-61 4, and miR-1913 is hsa-miR-1913, miR-16-5p is hsa-miR-16-5p, and miR-6717-5p is hsa-miR-671 7-5p, and miR-3648 is hsa-miR-3648, miR-316 2-5p is hsa-miR-3162-5p, miR-1909-3p is hsa- miR-1909-3p, miR-8073 is hsa-miR-8073, miR-6769b-5p is hsa-miR-6769b-5p, miR-683 6-3p is hsa-miR-6836-3p, miR-4484 is hsa-miR -4484, miR-6819-5p is hsa-miR-6819-5p, and miR-6794-5p is hsa-miR-6794-5p, the kit according to (1). (3) The nucleic acid is a polynucleotide represented by any of the following (a) to (e): (a) A polynucleotide consisting of a base sequence represented by any of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675, or a base sequence in which u is t in the base sequence, a variant thereof, a derivative thereof, or a fragment thereof containing 15 or more consecutive bases, (b) A polynucleotide containing a base sequence represented by any of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675, (c) A polynucleotide consisting of a base sequence complementary to a base sequence represented by any of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675, or a base sequence in which u is t in the base sequence, a variant thereof, a derivative thereof, or a fragment thereof containing 15 or more consecutive bases, (d) A polynucleotide containing a base sequence complementary to a base sequence represented by any of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675, or a base sequence in which u is t in the base sequence, and (e) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (f) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (g) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (h) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (i) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (j) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (k) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (l) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions (m) A polynucleotide that hybridizes with any of the polynucleotides of (a) to (d) under stringent conditions ​A polynucleotide that hybridizes The kit according to (1) or (2), which is a polynucleotide selected from the group consisting of (4) The kit further comprises a nucleic acid that can specifically bind to a polynucleotide consisting of miR-575 and miR-24-3 p, which is a nucleic acid that can specifically bind to a polynucleotide consisting of miR-575 and miR-24-3 The kit according to any one of (1) to (3). (5) miR-575 is hsa-miR-575, and miR-24-3p is hsa- The kit according to (4), wherein miR-24-3p is hsa-miR-24-3p (6) The nucleic acid is a polynucleotide shown in the following (f) to (j): (f) A polynucleotide consisting of the nucleotide sequences represented by SEQ ID NOs: 116 and 676 or a nucleotide sequence in which u is t, a variant thereof, a derivative thereof, or a fragment thereof containing 15 or more consecutive bases (g) A polynucleotide containing the nucleotide sequences represented by SEQ ID NOs: 116 and 676 (h) A polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NOs: 116 and 676 or a nucleotide sequence in which u is t, a variant thereof, a derivative thereof, or a fragment thereof containing 15 or more consecutive bases body, or a fragment thereof containing 15 or more consecutive bases (i) A polynucleotide containing a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NOs: 116 and 676 or a nucleotide sequence in which u is t, and (j) A polynucleotide that hybridizes with any of the polynucleotides in (f) to (i) under stringent conditions The kit according to (4) or (5), which is a polynucleotide selected from the group consisting of hybridizes (7) The kit is another esophageal cancer marker, miR-675-5p, miR-4 86-3p, miR-6777-5p, miR-4497, miR-296-3p, mi R-6738-5p, miR-4731-5p, miR-6889-5p, miR-67 86-5p, miR-92a-3p, miR-4294, miR-4763-3p, mi R-6076, miR-663a, miR-760, miR-4667-5p, miR- 6090, miR-4730, miR-7106-5p, miR-3196, miR-5 698, miR-6087, miR-4665-5p, miR-8059, and miR-6 at least one polynucleotide selected from the group consisting of 879-5p and capable of specifically binding to a nucleic acid, the kit according to any one of (1) to (6). (8) miR-675-5p is hsa-miR-675-5p, miR-486- 3p is hsa-miR-486-3p, miR-6777-5p is hsa-miR -6777-5p, miR-4497 is hsa-miR-4497, miR -296-3p is hsa-miR-296-3p, miR-6738-5p is hs a-miR-6738-5p, miR-4731-5p is hsa-miR-473 1-5p, miR-6889-5p is hsa-miR-6889-5p, m iR-6786-5p is hsa-miR-6786-5p, miR-92a-3p is hsa-miR-92a-3p, miR-4294 is hsa-miR-4294 and miR-4763-3p is hsa-miR-4763-3p, miR-6 076 is hsa-miR-6076, miR-663a is hsa-miR-663 a, miR-760 is hsa-miR-760, miR-4667-5p is hsa-miR-4667-5p, miR-6090 is hsa-miR-6090 where miR-4730 is hsa-miR-4730, miR-7106-5p is hsa-miR-7106-5p, miR-3196 is hsa-miR-319 6, miR-5698 is hsa-miR-5698, miR-6087 is h sa-miR-6087, miR-4665-5p is hsa-miR-4665- 5p, miR-8059 is hsa-miR-8059, and miR-68 79-5p is hsa-miR-6879-5p, the kit according to (7). (9) The nucleic acid is a polynucleotide represented by the following (k) to (o): (k) A polynucleotide consisting of a base sequence represented by any of SEQ ID NOs: 190 to 214 or a base sequence in which u is t in the base sequence, a variant thereof, a derivative thereof, or a fragment thereof containing 1 5 or more consecutive bases, (l) A polynucleotide containing a base sequence represented by any of SEQ ID NOs: 190 to 214, (m) A polynucleotide consisting of a base sequence complementary to the base sequence represented by any of SEQ ID NOs: 190 to 214 or a base sequence in which u is t in the base sequence, a variant thereof, (n) A polynucleotide consisting of a base sequence complementary to the base sequence represented by any of SEQ ID NOs: 190 to 214 or a base sequence in which u is t in the base sequence, a derivative thereof, or a fragment thereof containing 15 or more consecutive bases, (n) A polynucleotide containing a base sequence complementary to the base sequence represented by any of SEQ ID NOs: 190 to 214 or a base sequence in which u is t in the base sequence, and (o) A polynucleotide that hybridizes with any of the polynucleotides of (k) to (n) under stringent conditions, (n) A polynucleotide containing a base sequence complementary to the base sequence represented by any of SEQ ID NOs: 190 to 214 or a base sequence in which u is t in the base sequence, and (o) A polynucleotide that hybridizes with any of the polynucleotides of (k) to (n) under stringent conditions, (o) A polynucleotide that hybridizes with any of the polynucleotides of (k) to (n) under stringent conditions, hybridizes, The kit according to (7) or (8), which is a polynucleotide selected from the group consisting of (10) The kit is selected from all esophageal cancer markers described in (1) or (2) At least two nucleic acids each of which can specifically bind to each of at least two polynucleotides The kit according to any one of (1) to (9), comprising the nucleic acid (11) miR-204-3p, miR-1247-3p, m iR-6875-5p, miR-6857-5p, miR-6726-5p, miR-3 188, miR-8069, miR-4257, miR-1343-3p, miR-71 08-5p, miR-6825-5p, miR-7641, miR-3185, miR- 4746-3p, miR-6791-5p, miR-6893-5p, miR-4433 b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, m iR-4792, miR-7845-5p, miR-4417, miR-3184-5p 、miR-1225-5p, miR-1231, miR-1225-3p, miR-15 0-3p, miR-4433-3p, miR-6125, miR-4513, miR-6 787-5p, miR-6784-5p, miR-615-5p, miR-6765-3 p, miR-5572, miR-6842-5p, miR-8063, miR-6780 b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p 、miR-6741-5p, miR-6757-5p, miR-7110-5p, miR -7975, miR-1233-5p, miR-6845-5p, miR-3937, m iR-4467, miR-7109-5p, miR-6088, miR-6782-5p 、miR-5195-3p, miR-4454, miR-6724-5p, miR-80 72, miR-4516, miR-6756-5p, miR-4665-3p, miR- 6826-5p, miR-6820-5p, miR-6887-5p, miR-3679 -5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p , miR-939-5p, miR-602, miR-7977, miR-6749-5p , miR-1914-3p, miR-4651, miR-4695-5p, miR-68 48-5p, miR-1228-3p, miR-642b-3p, miR-6746-5 p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7 113-3p, miR-23a-3p, miR-3154, miR-4723-5p, m iR-3663-3p, miR-4734, miR-6816-5p, miR-4442 , miR-4476, miR-423-5p, miR-1249, miR-6515-3 p, miR-887-3p, miR-4741, miR-6766-3p, miR-46 73, miR-6779-5p, miR-4706, miR-1268b, miR-46 32-5p, miR-3197, miR-6798-5p, miR-711, miR-6 840-3p, miR-6763-5p, miR-6727-5p, miR-371a- 5p, miR-6824-5p, miR-4648, miR-1227-5p, miR- 564, miR-3679-3p, miR-2861, miR-6737-5p, miR -4725-3p, miR-6716-5p, miR-4675, miR-1915-3 p, miR-671-5p, miR-3656, miR-6722-3p, miR-47 07-5p, miR-4449, miR-1202, miR-4649-5p, miR- 744-5p, miR-642a-3p, miR-451a, miR-6870-5p, miR-4443, miR-6808-5p, miR-4728-5p, miR-937 -5p, miR-135a-3p, miR-663b, miR-1343-5p, miR -6822-5p, miR-6803-5p, miR-6805-3p, miR-128 -2-5p, miR-4640-5p, miR-1469, miR-92a-2-5p, miR-3940-5p, miR-4281, miR-1260b, miR-4758- 5p, miR-1915-5p, miR-5001-5p, miR-4286, miR- 6126, miR-6789-5p, miR-4459, miR-1268a, miR- 6752-5p, miR-6131, miR-6800-5p, miR-4532, mi R-6872-3p, miR-718, miR-6769a-5p, miR-4707- 3p, miR-6765-5p, miR-4739, miR-4525, miR-427 0, miR-4534, miR-6785-5p, miR-6850-5p, miR-4 697-5p, miR-1260a, miR-4486, miR-6880-5p, mi R-6802-5p, miR-6861-5p, miR-92b-5p, miR-123 8-5p, miR-6851-5p, miR-7704, miR-149-3p, miR -4689, miR-4688, miR-125a-3p, miR-23b-3p, mi R-614, miR-1913, miR-16-5p, miR-6717-5p, miR -3648, miR-3162-5p, miR-1909-3p, miR-8073, m iR-6769b-5p, miR-6836-3p, miR-4484, miR-681 At least one polynucleotide selected from the group consisting of 9-5p and miR-6794-5p A device for detecting esophageal cancer, comprising a nucleic acid capable of specifically binding to a polynucleotide (12) miR-204-3p is hsa-miR-204-3p, and miR-124 7-3p is hsa-miR-1247-3p, and miR-6875-5p is hsa- miR-6875-5p, and miR-6857-5p is hsa-miR-6857- 5p, and miR-6726-5p is hsa-miR-6726-5p, miR -3188 is hsa-miR-3188, and miR-8069 is hsa-miR-8 069, and miR-4257 is hsa-miR-4257, miR-1343 -3p is hsa-miR-1343-3p, and miR-7108-5p is hsa-m iR-7108-5p, and miR-6825-5p is hsa-miR-6825-5 p, and miR-7641 is hsa-miR-7641, miR-3185 is h sa-miR-3185, and miR-4746-3p is hsa-miR-4746- 3p, and miR-6791-5p is hsa-miR-6791-5p, miR -6893-5p is hsa-miR-6893-5p, and miR-4433b-3p is hsa-miR-4433b-3p, and miR-3135b is hsa-miR-3 135b, and miR-6781-5p is hsa-miR-6781-5p, m iR-1908-5p is hsa-miR-1908-5p, and miR-4792 is h sa-miR-4792, and miR-7845-5p is hsa-miR-7845- 5p, and miR-4417 is hsa-miR-4417, miR-3184- 5p is hsa-miR-3184-5p, miR-1225-5p is hsa-mi R-1225-5p, miR-1231 is hsa-miR-1231, mi R-1225-3p is hsa-miR-1225-3p, miR-150-3p is hsa-miR-150-3p, miR-4433-3p is hsa-miR-44 33-3p, miR-6125 is hsa-miR-6125, miR-45 13 is hsa-miR-4513, miR-6787-5p is hsa-miR-6 787-5p, miR-6784-5p is hsa-miR-6784-5p and miR-615-5p is hsa-miR-615-5p, miR-6765-3 p is hsa-miR-6765-3p, miR-5572 is hsa-miR-55 72, miR-6842-5p is hsa-miR-6842-5p, miR -8063 is hsa-miR-8063, miR-6780b-5p is hsa-m iR-6780b-5p, miR-187-5p is hsa-miR-187-5p and miR-128-1-5p is hsa-miR-128-1-5p, miR -6729-5p is hsa-miR-6729-5p, miR-6741-5p is hsa-miR-6741-5p, miR-6757-5p is hsa-miR-6 757-5p, miR-7110-5p is hsa-miR-7110-5p and miR-7975 is hsa-miR-7975, miR-1233-5p is hs a-miR-1233-5p, miR-6845-5p is hsa-miR-684 5-5p, miR-3937 is hsa-miR-3937, miR-446 7 is hsa-miR-4467, miR-7109-5p is hsa-miR-71 09-5p, miR-6088 is hsa-miR-6088, miR-67 82-5p is hsa-miR-6782-5p, miR-5195-3p is hsa -miR-5195-3p, miR-4454 is hsa-miR-4454 , miR-6724-5p is hsa-miR-6724-5p, miR-8072 is hsa-miR-8072, miR-4516 is hsa-miR-4516 , miR-6756-5p is hsa-miR-6756-5p, miR-466 5-3p is hsa-miR-4665-3p, miR-6826-5p is hsa- miR-6826-5p, miR-6820-5p is hsa-miR-6820- 5p, miR-6887-5p is hsa-miR-6887-5p, miR -3679-5p is hsa-miR-3679-5p, miR-7847-3p is hsa-miR-7847-3p, miR-6721-5p is hsa-miR-6 721-5p, miR-3622a-5p is hsa-miR-3622a-5p and, miR-939-5p is hsa-miR-939-5p, miR-602 is hsa-miR-602, miR-7977 is hsa-miR-7977, miR-6749-5p is hsa-miR-6749-5p, miR-1914- 3p is hsa-miR-1914-3p, miR-4651 is hsa-miR-4 651, miR-4695-5p is hsa-miR-4695-5p, mi R-6848-5p is hsa-miR-6848-5p, miR-1228-3p is hsa-miR-1228-3p, miR-642b-3p is hsa-miR- 642b-3p, miR-6746-5p is hsa-miR-6746-5p, miR-3620-5p is hsa-miR-3620-5p, miR-313 1 is hsa-miR-3131, miR-6732-5p is hsa-miR-67 32-5p, miR-7113-3p is hsa-miR-7113-3p, miR-23a-3p is hsa-miR-23a-3p, miR-3154 is hs a-miR-3154, miR-4723-5p is hsa-miR-4723-5 p, miR-3663-3p is hsa-miR-3663-3p, miR- 4734 is hsa-miR-4734, miR-6816-5p is hsa-miR -6816-5p, miR-4442 is hsa-miR-4442, miR -4476 is hsa-miR-4476, miR-423-5p is hsa-miR -423-5p, miR-1249 is hsa-miR-1249, miR- 6515-3p is hsa-miR-6515-3p, miR-887-3p is hs a-miR-887-3p, miR-4741 is hsa-miR-4741 , miR-6766-3p is hsa-miR-6766-3p, miR-4673 is hsa-miR-4673, miR-6779-5p is hsa-miR-677 9-5p, miR-4706 is hsa-miR-4706, miR-126 8b is hsa-miR-1268b, miR-4632-5p is hsa-miR- It is 4632-5p, miR-3197 is hsa-miR-3197, miR- 6798-5p is hsa-miR-6798-5p, miR-711 is hsa-m iR-711, miR-6840-3p is hsa-miR-6840-3p , miR-6763-5p is hsa-miR-6763-5p, miR-6727 -5p is hsa-miR-6727-5p, miR-371a-5p is hsa-m iR-371a-5p, miR-6824-5p is hsa-miR-6824-5 p, miR-4648 is hsa-miR-4648, miR-1227-5 p is hsa-miR-1227-5p, miR-564 is hsa-miR-564 and miR-3679-3p is hsa-miR-3679-3p, miR-2 861 is hsa-miR-2861, miR-6737-5p is hsa-miR- 6737-5p, miR-4725-3p is hsa-miR-4725-3p and miR-6716-5p is hsa-miR-6716-5p, miR-467 5 is hsa-miR-4675, miR-1915-3p is hsa-miR-19 15-3p, miR-671-5p is hsa-miR-671-5p, mi R-3656 is hsa-miR-3656, miR-6722-3p is hsa-m iR-6722-3p, miR-4707-5p is hsa-miR-4707-5 p, miR-4449 is hsa-miR-4449, miR-1202 is h sa-miR-1202, miR-4649-5p is hsa-miR-4649- It is 5p, miR-744-5p is hsa-miR-744-5p, and miR-6 42a-3p is hsa-miR-642a-3p, and miR-451a is hsa-m iR-451a, miR-6870-5p is hsa-miR-6870-5p, and miR-4443 is hsa-miR-4443, miR-6808-5p is h sa-miR-6808-5p, miR-4728-5p is hsa-miR-47 28-5p, miR-937-5p is hsa-miR-937-5p, and mi R-135a-3p is hsa-miR-135a-3p, miR-663b is hs a-miR-663b, miR-1343-5p is hsa-miR-1343-5 p, miR-6822-5p is hsa-miR-6822-5p, and miR- 6803-5p is hsa-miR-6803-5p, miR-6805-3p is h sa-miR-6805-3p, miR-128-2-5p is hsa-miR-1 28-2-5p, miR-4640-5p is hsa-miR-4640-5p, and miR-1469 is hsa-miR-1469, miR-92a-2-5p is hsa-miR-92a-2-5p, miR-3940-5p is hsa-miR- 3940-5p, miR-4281 is hsa-miR-4281, and miR- 1260b is hsa-miR-1260b, miR-4758-5p is hsa-m iR-4758-5p, miR-1915-5p is hsa-miR-1915-5 p, miR-5001-5p is hsa-miR-5001-5p, and miR- 4286 is hsa-miR-4286, miR-6126 is hsa-miR-61 is 26, miR-6789-5p is hsa-miR-6789-5p, miR -4459 is hsa-miR-4459, miR-1268a is hsa-miR- 1268a, miR-6752-5p is hsa-miR-6752-5p, miR-6131 is hsa-miR-6131, miR-6800-5p is hsa -miR-6800-5p, miR-4532 is hsa-miR-4532 , miR-6872-3p is hsa-miR-6872-3p, miR-718 is hsa-miR-718, miR-6769a-5p is hsa-miR-6769 a-5p, miR-4707-3p is hsa-miR-4707-3p, m iR-6765-5p is hsa-miR-6765-5p, miR-4739 is h sa-miR-4739, miR-4525 is hsa-miR-4525, miR-4270 is hsa-miR-4270, miR-4534 is hsa-mi R-4534, miR-6785-5p is hsa-miR-6785-5p , miR-6850-5p is hsa-miR-6850-5p, miR-4697 -5p is hsa-miR-4697-5p, miR-1260a is hsa-miR -1260a, miR-4486 is hsa-miR-4486, miR-6 880-5p is hsa-miR-6880-5p, miR-6802-5p is hs a-miR-6802-5p, miR-6861-5p is hsa-miR-686 1-5p, miR-92b-5p is hsa-miR-92b-5p, miR -1238-5p is hsa-miR-1238-5p, miR-6851-5p is hsa-miR-6851-5p, miR-7704 is hsa-miR-7704 and miR-149-3p is hsa-miR-149-3p, miR-468 9 is hsa-miR-4689, miR-4688 is hsa-miR-4688 and miR-125a-3p is hsa-miR-125a-3p, miR-23 b-3p is hsa-miR-23b-3p, miR-614 is hsa-miR-6 14, miR-1913 is hsa-miR-1913, miR-16-5p is hsa-miR-16-5p, miR-6717-5p is hsa-miR-67 17-5p, miR-3648 is hsa-miR-3648, miR-31 62-5p is hsa-miR-3162-5p, miR-1909-3p is hsa -miR-1909-3p, miR-8073 is hsa-miR-8073 and miR-6769b-5p is hsa-miR-6769b-5p, miR-68 36-3p is hsa-miR-6836-3p, miR-4484 is hsa-mi R-4484, miR-6819-5p is hsa-miR-6819-5p and miR-6794-5p is hsa-miR-6794-5p, (11) The device according to. (13) The nucleic acid is a polynucleotide represented by any of the following (a) to (e): (a) A base sequence represented by any of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a polynucleotide consisting of a base sequence in which u is t in the base sequence or a polynucleotide consisting of a base sequence in which u is t in the base sequence D, its variants, its derivatives, or fragments thereof containing 15 or more consecutive bases, (b) a polynucleotide comprising the nucleotide sequence represented by any one of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675, (c) a polynucleotide comprising the nucleotide sequence represented by any one of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a nucleotide sequence complementary to the nucleotide sequence in which u is t in the said nucleotide sequence, its variants, its derivatives, or fragments thereof containing 15 or more consecutive bases, (d) a polynucleotide comprising the nucleotide sequence represented by any one of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a nucleotide sequence complementary to the nucleotide sequence in which u is t in the said nucleotide sequence, and (e) a polynucleotide that hybridizes under stringent conditions with any one of the polynucleotides of (a) to (d) above, and is a polynucleotide selected from the group consisting of: (11) or (12) above. (14) The device further comprises a nucleic acid that can specifically bind to a polynucleotide consisting of miR-575 and miR-24-3p, which are another esophageal cancer marker. (11) to any one of (13) above. (15) The device according to (14) above, wherein miR-575 is hsa-miR-575 and miR-24-3p is hs a-miR-24-3p. (16) The nucleic acid is a polynucleotide shown in the following (f) to (j): (f) a polynucleotide consisting of the nucleotide sequence represented by SEQ ID NOs: 116 and 676 or a nucleotide sequence in which u is t in the said nucleotide sequence, its variants, its derivatives, or fragments thereof containing 15 or more consecutive bases, ​​​​​​​​​(g) A polynucleotide comprising the nucleotide sequences represented by SEQ ID NOs: 116 and 676, (h) A polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NOs: 116 and 676 or a nucleotide sequence in which u is t, its variants, its derivatives, or a fragment thereof containing 15 or more consecutive bases, (i) A polynucleotide containing the nucleotide sequence represented by SEQ ID NOs: 116 and 676 or a nucleotide sequence complementary to the nucleotide sequence in which u is t, and (j) A polynucleotide that hybridizes under stringent conditions with any of the polynucleotides of (f) to (i) above, is a polynucleotide selected from the group consisting of: The device according to (14) or (15). (17) The device further comprises a nucleic acid that can specifically bind to at least one polynucleotide selected from the group consisting of miR-675-5p, miR -486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, miR-6889-5p, miR- 6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, mi R-6090, miR-4730, miR-7106-5p, miR-3196, miR -5698, miR-6087, miR-4665-5p, miR-8059 and miR -6879-5p, which are other esophageal cancer markers. The device according to any of (11) to (16). (18) miR-675-5p is hsa-miR-675-5p, miR-486 -3p is hsa-miR-486-3p, miR-6777-5p is hsa-mi R-6777-5p, miR-4497 is hsa-miR-4497, mi R-296-3p is hsa-miR-296-3p, miR-6738-5p is h sa-miR-6738-5p, miR-4731-5p is hsa-miR-47 31-5p, miR-6889-5p is hsa-miR-6889-5p, miR-6786-5p is hsa-miR-6786-5p, miR-92a-3 p is hsa-miR-92a-3p, miR-4294 is hsa-miR-429 4, miR-4763-3p is hsa-miR-4763-3p, miR- 6076 is hsa-miR-6076, miR-663a is hsa-miR-66 3a, miR-760 is hsa-miR-760, miR-4667-5p is hsa-miR-4667-5p, miR-6090 is hsa-miR-609 0, miR-4730 is hsa-miR-4730, miR-7106-5 p is hsa-miR-7106-5p, miR-3196 is hsa-miR-31 96, miR-5698 is hsa-miR-5698, miR-6087 is hsa-miR-6087, miR-4665-5p is hsa-miR-4665 -5p, miR-8059 is hsa-miR-8059, and miR-6 879-5p is hsa-miR-6879-5p, the device according to (17). (19) The nucleic acid is a polynucleotide shown in the following (k) to (o): (k) a base sequence represented by any of SEQ ID NOs: 190 to 214 or in the base sequence a polynucleotide consisting of a base sequence in which u is t, a variant thereof, a derivative thereof, or a fragment thereof containing 5 or more consecutive bases; (l) a polynucleotide comprising a nucleotide sequence represented by any one of SEQ ID NOs: 190 to 214; (m) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide consisting of a base sequence complementary to the base sequence in which u is t, a variant thereof, its derivatives or fragments containing 15 or more consecutive bases; (n) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide comprising a base sequence complementary to the base sequence in which u is t; (o) a polynucleotide that binds to any one of the polynucleotides (k) to (n) under stringent conditions; hybridizing polynucleotides, The device according to (17) or (18), which is a polynucleotide selected from the group consisting of Chair. (20) The device is a device for measurement by hybridization technology. The device according to any one of (11) to (19). (21) The method according to (20), wherein the hybridization technique is a nucleic acid array technique. device. (22) The device comprises: At least one polynucleotide capable of specifically binding to each of at least two selected polynucleotides. The device according to any one of (11) to (21), comprising at least two nucleic acids. (23) The kit according to any one of (1) to (10) or (11) to (22). The expression level of a target nucleic acid in a specimen of a subject is measured using the device described in The expression levels measured in the test samples and the control expression levels measured in the test samples were compared to determine whether the test sample was affected by esophageal cancer. evaluating in vitro whether the subject has esophageal cancer or not, or whether the subject does not have esophageal cancer A method for detecting esophageal cancer, comprising the above. (24) The method according to (23), wherein the subject is a human. (25) The method according to (23) or (24), wherein the sample is blood, serum or plasma. [Definition of terms] The terms used in this specification have the following definitions.

[0018] The representations by abbreviations such as nucleotide, polynucleotide, DNA, RNA, etc. shall follow the "Guidelines for the Preparation of Specifications, etc. Containing Base Sequences or Amino Acid Sequences" (edited by the Japan Patent Office) and the common practice in the relevant technical field. and the common practice in the relevant technical field. In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification

[0019] In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "polynucleotide" is used for nucleic acids including any of RNA, DNA, and RNA / DNA (chimera). The above DNA includes cDNA, genomic DNA, and synthetic DNA. The above RNA includes total RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, non-coding RNA, and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to DNA and RNA artificially produced, for example, using an automatic nucleic acid synthesizer, based on a predetermined base sequence (which may be either a natural sequence or a non-natural sequence). In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification In this specification, the term "non-natural sequence" is intended to be used in a broad sense and includes sequences different from natural sequences, for example, sequences containing substitutions, deletions, insertions, and / or additions of one or more nucleotides (i.e., mutant sequences), sequences containing one or more modified nucleotides (i.e., modified sequences), etc. Also, in this specification A polynucleotide is used interchangeably with a nucleic acid.

[0020] As used herein, a "fragment" is a polynucleotide having the nucleotide sequence of a continuous portion of a polynucleotide, and desirably has a length of 15 bases or more, preferably 17 bases or more, more preferably 1 9 bases or more.

[0021] As used herein, the term "gene" is intended to include not only RNA and double-stranded DNA, but also single-stranded DNA such as the positive strand (or sense strand) or complementary strand (or antisense strand) that constitutes them. It is not particularly limited by its length. Accordingly, as used herein, the term "gene" refers to double-stranded DNA containing human genomic DNA, single-stranded DNA (positive strand), single-stranded DNA having a sequence complementary to the positive strand (complementary strand, including cDNA), microRNA (miRNA), and fragments thereof, unless otherwise specified. Also included are any of their transcripts. Further, the "gene" is not limited to the "gene" represented by a specific nucleotide sequence (or sequence number), but also includes RNA encoded by these and RNA having equivalent biological functions, such as homologs (i.e., homologs or orthologs), mutants such as gene polymorphisms, and derivatives. Examples of the "nucleic acid" encoding such homologs, mutants or derivatives include, specifically, a nucleotide sequence that hybridizes under stringent conditions described later with the nucleotide sequence represented by any of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the nucleotide sequence, and its complementary sequence. Note that the "gene" is not limited by the distinction of functional regions. For example, it may contain an expression control region, a coding region, an exon or an intron. Also the "gene" may be contained in a cell, may be released extracellularly and exist alone, or may be encapsulated in vesicles called exosomes.

[0022] In this specification, an "exosome" is a vesicle encapsulated by a lipid bilayer secreted from a cell. Exosomes are derived from multivesicular endosomes and may contain biological substances such as "genes" such as RNA, DNA, etc. and proteins when released into the extracellular environment. Exosomes are known to be contained in body fluids such as blood, serum, plasma, and lymph. Also, exosomes are sometimes generally referred to as "exosomes".

[0023] In this specification, the "transcript" refers to RNA synthesized using the DNA sequence of a gene as a template. RNA polymerase binds to a site called a promoter upstream of the gene, and RNA is synthesized in such a way that ribonucleotides are bound to the 3' end to be complementary to the base sequence of the DNA. This RNA contains not only the gene itself, but also the entire sequence from the transcription start point including the expression control region, the coding region, the exon or the intron to the end of the polyA sequence.

[0024] Also, in this specification, "microRNA (miRNA)" is, unless otherwise specified, transcribed as an RNA precursor with a hairpin-like structure, cleaved by a dsRNA cleavage enzyme having RNase III cleavage activity, incorporated into a protein complex called RISC, and used to intend a 15-25 base non-coding RNA involved in translational repression of mRNA. ​

[0025] In addition, the "miRNA" used in this specification is represented by a specific base sequence (or SEQ ID NO). It includes not only "miRNA" but also precursors of the "miRNA" (pre-miRNA, pri-miRNA), miRNAs having biological functions equivalent to these, for example, homologs (that is, homologs or orthologs), mutants such as gene polymorphisms, and derivatives. Among such precursors, homologs, mutants or derivatives, specifically, those that can be identified by miRBase release 20 (http: / / www.mirbase.org / ) and hybridize with the complementary sequence of any specific base sequence represented by any of SEQ ID NOs: 1 to 700 under the stringent conditions described later can be mentioned as "miRNA". Furthermore, the "miRNA" used in this specification may be a gene product of an miR gene, and such a gene product includes mature miRNA (for example, 15 to 25 bases or 19 to 25 bases of non-coding RNA involved in translational repression of mRNA as described above) or miRNA precursor (for example, pre-miRNA or pri-miRNA as described above). In this specification, a "probe" includes a polynucleotide used for specifically detecting an RNA generated by gene expression or a polynucleotide derived therefrom and / or a polynucleotide complementary thereto. In this specification, a "primer" includes a polynucleotide that specifically recognizes and amplifies an RNA generated by gene expression or a polynucleotide derived therefrom and / or

[0026]

[0027] ​​​​​​​​​​​​​ comprises a polynucleotide complementary thereto. Here, the complementary polynucleotide (complementary strand, reverse strand) means a polynucleotide having a nucleotide sequence defined by any one of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the said nucleotide sequence, the full-length sequence of the polynucleotide consisting thereof, or a partial sequence thereof, (hereinafter referred to as the sense strand for convenience), and a polynucleotide having a base complementarity based on the base pair relationship of A:T(U), G:C with respect to it. However, such a complementary strand is not limited to forming a completely complementary sequence with the nucleotide sequence of the target sense strand, and may have a complementary relationship that can hybridize with the target sense strand under stringent conditions. In the present specification, "stringent conditions" means conditions under which a nucleic acid probe hybridizes to its target sequence to a greater extent (for example, a measured value of the average of background measurement values + standard error of background measurement values × 2 or more) than to other sequences. Stringent conditions are sequence-dependent and vary depending on the environment in which hybridization is performed. By controlling the stringency of hybridization and / or washing conditions, a target sequence that is 100% complementary to the nucleic acid probe can be identified. Specific examples of "stringent conditions" will be described later. In the present specification, the "Tm value" means the temperature at which the double-stranded portion of a polynucleotide denatures into a single strand and the double strand and the single strand are present in a ratio of 1:1. In the present specification, "variant" in the case of nucleic acids means a natural one caused by polymorphism, mutation, etc.

[0028]

[0029]

[0030] ​​​​​​​​​​​​A variant thereof, or a nucleotide sequence of any one of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the above, or a variant including deletion, substitution, addition or insertion of one or more bases in a partial sequence thereof, or a variant showing identity of about 90% or more, about 95% or more, about 97% or more, about 98% or more, about 99% or more with each of the above nucleotide sequences or a partial sequence thereof, or a polynucleotide or oligonucleotide including the above nucleotide sequence or a partial sequence thereof, and a nucleic acid that hybridizes under stringent conditions defined above. A nucleotide sequence in which u is t in the above, or a variant including deletion, substitution, addition or insertion of one or more bases in a partial sequence thereof A variant thereof, or a nucleotide sequence of any one of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the above, or a variant including deletion, substitution, addition or insertion of one or more bases in a partial sequence thereof, or a variant showing identity of about 90% or more, about 95% or more, about 97% or more, about 98% or more, about 99% or more with each of the above nucleotide sequences or a partial sequence thereof A variant thereof, or a nucleotide sequence of any one of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the above, or a variant including deletion, substitution, addition or insertion of one or more bases in a partial sequence thereof, or a variant showing identity of about 90% or more, about 95% or more, about 97% or more, about 98% or more, about 99% or more with each of the above nucleotide sequences or a partial sequence thereof A variant thereof, or a nucleotide sequence of any one of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the above, or a variant including deletion, substitution, addition or insertion of one or more bases in a partial sequence thereof, or a variant showing identity of about 90% or more, about 95% or more, about 97% or more, about 98% or more, about 99% or more with each of the above nucleotide sequences or a partial sequence thereof, or a polynucleotide or oligonucleotide including the above nucleotide sequence or a partial sequence thereof, and a nucleic acid that hybridizes under stringent conditions defined above. A variant thereof, or a nucleotide sequence of any one of SEQ ID NOs: 1 to 700, or a nucleotide sequence in which u is t in the above, or a variant including deletion, substitution, addition or insertion of one or more bases in a partial sequence thereof, or a variant showing identity of about 90% or more, about 95% or more, about 97% or more, about 98% or more, about 99% or more with each of the above nucleotide sequences or a partial sequence thereof, or a polynucleotide or oligonucleotide including the above nucleotide sequence or a partial sequence thereof, and a nucleic acid that hybridizes under stringent conditions defined above.

[0031] As used herein, "a plurality" means an integer of about 10, 9, 8, 7, 6, 5, 4, 3 or 2. As used herein, "a plurality" means an integer of about 10, 9, 8, 7, 6, 5, 4, 3 or 2.

[0032] As used herein, a variant can be prepared using well-known techniques such as site-directed mutagenesis and mutagenesis introduction methods using the PCR method. As used herein, a variant can be prepared using well-known techniques such as site-directed mutagenesis and mutagenesis introduction methods using the PCR method.

[0033] As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448). As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448). As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448). As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448). As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448). As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448). As used herein, "identity" can be determined using the above protein or gene search systems such as BLAST and FASTA, with or without introducing gaps (Zheng Zhang et al., 2000, J. Comput. Biol., 7, p203-214; Altschul, S.F. et al., 1990, Journal of Molecular Biology, Vol. 215, p403-410; Pearson, W.R. et al., 1988, Proc. Natl. Acad. Sci. U.S.A., 85, p2444-2448).

[0034] As used herein, "derivative" refers to a modified nucleic acid, including, but not limited to, labeling with a fluorophore, etc. Bell-shaped derivatives, modified nucleotides (e.g., nucleotides containing groups such as alkyls such as halogen and methyl, alkoxys such as methoxy, thio, carboxymethyl, etc.) and reconfigured bases and nucleotides and bases that have undergone saturation of double bonds, deamination, substitution of oxygen molecules with sulfur molecules, etc. Derivatives containing such), PNA (peptide nucleic acid; Niels en, P.E. et al., 1991, Science, Vol. 254, p1497 - 500), L NA (locked nucleic acid; Obika, S. et al., 1998, T etrahedron Lett., Vol. 39, p5401 - 5404), etc. This means including etc. Meaning.

[0035] In this specification, the "nucleic acid" that can specifically bind to a polynucleotide selected from the above esophageal cancer marker miRNAs is a synthesized or prepared nucleic acid, specifically including "nucleic acid probe" or "primer", and is used directly or indirectly to detect the presence or absence of esophageal cancer in a subject, or to diagnose the presence or absence of esophageal cancer, the degree of disease, the presence or absence and degree of improvement of esophageal cancer, the sensitivity to the treatment of esophageal cancer, or to screen for candidate substances useful for the prevention, improvement or treatment of esophageal cancer. These include transcripts represented by any of SEQ ID NOs: 1 - 700 or their cDNA - synthesized nucleic acids in vivo in relation to the development of esophageal cancer, particularly in specimens such as body fluids such as blood and urine, and specifically recognize and bind nucleotides, oligonucleotides and polynucleotides that can do so. These nucleotides, oligonucleotides and polynucleotides are used as probes for detecting the above - mentioned genes expressed in vivo, in tissues, cells, etc. based on the above properties, and are included. 1 - 700 or their cDNA - synthesized nucleic acids in vivo in relation to the development of esophageal cancer, particularly in specimens such as body fluids such as blood and urine, and specifically recognize and bind nucleotides, oligonucleotides and polynucleotides that can do so. These nucleotides, oligonucleotides and polynucleotides are used as probes for detecting the above - mentioned genes expressed in vivo, in tissues, cells, etc. based on the above properties, . These nucleotides, oligonucleotides and polynucleotides are, based on the above properties, used as probes for detecting the above - mentioned genes expressed in vivo, in tissues, cells, etc., It can be effectively used as a primer for amplifying the gene expressed in vivo. This can be done.

[0036] As used herein, the term "detection" can be replaced with terms such as inspection, measurement, detection, or judgment assistance. Also, as used herein, the term "evaluation" is used in the sense of including assisting in diagnosis or evaluation based on inspection results or measurement results. As used herein, the term "evaluation" is used in the sense of including assisting in diagnosis or evaluation based on inspection results or measurement results. Based on the inspection results or measurement results, it is used to assist in diagnosis or evaluation.

[0037] As used herein, the term "subject" means mammals such as humans, primates including chimpanzees, pet animals such as dogs and cats, livestock animals such as cows, horses, sheep, and goats, and rodents such as mice and rats. Also, "healthy subject" also means such a mammal that does not suffer from the cancer to be detected, that is, esophageal cancer. As used herein, the term "subject" means mammals such as humans, primates including chimpanzees, pet animals such as dogs and cats, livestock animals such as cows, horses, sheep, and goats, and rodents such as mice and rats. Also, "healthy subject" also means such a mammal that does not suffer from the cancer to be detected, that is, esophageal cancer. As used herein, the term "subject" means mammals such as humans, primates including chimpanzees, pet animals such as dogs and cats, livestock animals such as cows, horses, sheep, and goats, and rodents such as mice and rats. Also, "healthy subject" also means such a mammal that does not suffer from the cancer to be detected, that is, esophageal cancer. As used herein, the term "subject" means mammals such as humans, primates including chimpanzees, pet animals such as dogs and cats, livestock animals such as cows, horses, sheep, and goats, and rodents such as mice and rats. Also, "healthy subject" also means such a mammal that does not suffer from the cancer to be detected, that is, esophageal cancer.

[0038] As used herein, "P" or "P value" refers to the probability that a statistic more extreme than the statistic actually calculated from the data is observed under the null hypothesis in a statistical test. Thus, the smaller "P" or "P value" is, the more likely there is a significant difference between the comparison subjects. As used herein, "P" or "P value" refers to the probability that a statistic more extreme than the statistic actually calculated from the data is observed under the null hypothesis in a statistical test. Thus, the smaller "P" or "P value" is, the more likely there is a significant difference between the comparison subjects. Therefore, the smaller "P" or "P value" is, the more likely there is a significant difference between the comparison subjects.

[0039] As used herein, "sensitivity" means the value of (number of true positives) / (number of true positives + number of false negatives). If the sensitivity is high, it becomes possible to detect esophageal cancer at an early stage, leading to the resection of the entire cancerous part and a reduction in the recurrence rate. As used herein, "sensitivity" means the value of (number of true positives) / (number of true positives + number of false negatives). If the sensitivity is high, it becomes possible to detect esophageal cancer at an early stage, leading to the resection of the entire cancerous part and a reduction in the recurrence rate. If the sensitivity is high, it becomes possible to detect esophageal cancer at an early stage, leading to the resection of the entire cancerous part and a reduction in the recurrence rate.

[0040] As used herein, "specificity" means (number of true negatives) / (number of true negatives + number of false positives). If the specificity is high, it prevents the unnecessary additional examinations due to misclassifying healthy subjects as esophageal cancer patients, leading to a reduction in the burden on patients and medical costs. As used herein, "specificity" means (number of true negatives) / (number of true negatives + number of false positives). If the specificity is high, it prevents the unnecessary additional examinations due to misclassifying healthy subjects as esophageal cancer patients, leading to a reduction in the burden on patients and medical costs. If the specificity is high, it prevents the unnecessary additional examinations due to misclassifying healthy subjects as esophageal cancer patients, leading to a reduction in the burden on patients and medical costs.

[0041] In this specification, "accuracy" means the value of (the number of true positives + the number of true negatives) / (the total number of cases). Accuracy indicates the proportion of correct discrimination results for all specimens, and is the first index for evaluating detection performance.

[0042] In this specification, the "specimen" to be determined, detected or diagnosed refers to tissues and biological materials in which the gene of the present invention undergoes expression changes along with the occurrence, progression of esophageal cancer, and the exertion of therapeutic effects on esophageal cancer. Specifically, it refers to esophageal tissues and their surrounding blood vessels, lymph nodes and organs, as well as organs suspected of metastasis, skin, and body fluids such as blood, urine, saliva, sweat, tissue exudates, etc. Serum, plasma prepared from blood, and others, feces, hair, etc. Furthermore, biological samples extracted from these, specifically genes such as RNA and miRNA are referred to.

[0043] The term "hsa-miR-204-3p gene" or "hsa-miR-204-3p" used in this specification includes the hsa-miR-204-3p gene (miRBase Accession No. MIMAT0022693) consisting of the nucleotide sequence represented by SEQ ID NO: 1, and other species homologs or orthologs, etc. The hsa-miR-204-3p gene can be obtained by the method described in Lim LP et al., 2003, Science, Vol. 299, p1540. Also, "hsa-miR-204-3p" has as its precursor "hsa-mir-204" (miRBase Accession No. MI0000284, SEQ ID NO: 215) which has a hairpin-like structure and is known.

[0044] ​​​​​​​​​​​As used herein, the term "hsa-miR-1247-3p gene" or "hsa-mi R-1247-3p" includes the hsa-m iR-1247-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 2 (miRBase Accession No. MIMAT 0022721), as well as homologs or orthologs of other species. The hsa- miR-1247-3p gene can be obtained by the method described in Morin RD et al., 2008, Genome R es, 18, p610-621. Also, " hsa-miR-1247-3p" has a hairpin-like structure as its precursor, "hsa -mir-1247" (miRBase Accession No. MI000638 2, SEQ ID NO: 216) is known.

[0045] As used herein, the term "hsa-miR-6875-5p gene" or "hsa-mi R-6875-5p" includes the hsa-m iR-6875-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 3 (miRBase Accession No. MIMAT 0027650), as well as homologs or orthologs of other species. The hsa- miR-6875-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6875-5p" has a hairpin-like structure as its precursor, " hsa-mir-6875" (miRBase Accession No. MI002 2722, SEQ ID NO: 217) is known.

[0046] As used herein, the term "hsa-miR-6857-5p gene" or "hsa-mi The term "R-6857-5p" refers to hsa-m consisting of the nucleotide sequence represented by SEQ ID NO: 4 iR-6857-5p gene (miRBase Accession No. MIMAT 0027614) and other species homologs or orthologs, etc. are included. hsa- The miR-6857-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6857-5p" has a hairpin-like structure as its precursor, " hsa-mir-6857" (miRBase Accession No. MI002 2703, SEQ ID NO: 218) is known.

[0047] As used herein, the term "hsa-miR-6726-5p gene" or "hsa-mi R-6726-5p" refers to hsa-m consisting of the nucleotide sequence represented by SEQ ID NO: 5 iR-6726-5p gene (miRBase Accession No. MIMAT 0027353) and other species homologs or orthologs, etc. are included. hsa- The miR-6726-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6726-5p" has a hairpin-like structure as its precursor, " hsa-mir-6726" (miRBase Accession No. MI002 2571, SEQ ID NO: 219) is known.

[0048] As used herein, the term "hsa-miR-3188 gene" or "hsa-miR-3 The term "188" refers to hsa-miR-3188 consisting of the nucleotide sequence represented by SEQ ID NO: 6 8 genes (miRBase Accession No. MIMAT0015070), and also includes other species homologs or orthologs, etc. The hsa-miR-3188 gene can be obtained by the method described by Stark MS et al., 2010, PLoS One, Vol. 5, e9685. Also, "hsa-miR-3188" has a hairpin-like structure as its precursor "hsa-mir-3188" (miRBase Ac cession No. MI0014232, SEQ ID NO: 220) is known.

[0049] As used herein, the term "hsa-miR-8069 gene" or "hsa-miR-8 069" refers to hsa-miR-8069 gene consisting of the nucleotide sequence represented by SEQ ID NO: 7 9 genes (miRBase Accession No. MIMAT0030996), and also includes other species homologs or orthologs, etc. The hsa-miR-8069 gene can be obtained by the method described by Wang HJ et al., 2013, Shock, Vol. 39, p480-487. Also, "hsa-miR-8069" has a hairpin-like structure as its precursor "hsa-mir-8069" (miRBase Ac cession No. MI0025905, SEQ ID NO: 221) is known.

[0050] As used herein, the term "hsa-miR-4257 gene" or "hsa-miR-4 257" refers to hsa-miR-4257 gene consisting of the nucleotide sequence represented by SEQ ID NO: 8 7 genes (miRBase Accession No. MIMAT0016878), and It includes other species homologs or orthologs, etc. The hsa-miR-4257 gene can be obtained by the method described by Goff LA et al., 2009, PLoS One, Vol. 4, e7192. Also, "hsa-miR-4257" has a hairpin-like structure as its precursor, "hsa-mir-4257" (miRBase Acc ession No. MI0015856, SEQ ID NO: 222).

[0051] As used herein, the term "hsa-miR-1343-3p gene" or "hsa-mi R-1343-3p" refers to the hsa-miR-1343-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 9 (miRBase Accession No. MIMAT 0019776) and includes other species homologs or orthologs, etc. The hsa- miR-1343-3p gene can be obtained by the method described by Persson H et al., 2011, Cancer Res, Vol. 71, p78-86. Also, "h sa-miR-1343-3p" has a hairpin-like structure as its precursor, "hsa- mir-1343" (miRBase Accession No. MI0017320 , SEQ ID NO: 223).

[0052] As used herein, the term "hsa-miR-7108-5p gene" or "hsa-mi R-7108-5p" refers to the hsa-miR-7108-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 10 (miRBase Accession No. MIMA T0028113) and includes other species homologs or orthologs, etc. Hsa -miR-7108-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-7108-5p" takes a hairpin-like structure as its precursor "hsa-mir-7108" (miRBase Accession No.MI00 22959, SEQ ID NO: 224) is known.

[0053] As used herein, the term "hsa-miR-6825-5p gene" or "hsa-mi R-6825-5p" includes the hsa-miR-6825-5p gene (miRBase Accession No.MIMAT0027550) consisting of the nucleotide sequence represented by SEQ ID NO: 11 and other species homologs or orthologs, etc. The hsa -miR-6825-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6825-5p" takes a hairpin-like structure as its precursor "hsa-mir-6825" (miRBase Accession No.MI00 22670, SEQ ID NO: 225) is known.

[0054] As used herein, the term "hsa-miR-7641 gene" or "hsa-miR-7 641" includes the hsa-miR-7641 gene (miRBase Accession No.MIMAT0029782) consisting of the nucleotide sequence represented by SEQ ID NO: 12 and other species homologs or orthologs, etc. The hsa-miR-7641 ​​The gene can be obtained by the method described in Yoo JK et al., 2013, Arch Pharm Res, 36, p3 53 - 358. Also, "hsa - miR - 7 641" has, as its precursor, "hsa - mir - 7641 - 1, hsa - mir - 7641 - 2" (miRBase Accession No.MI0 024975, MI0024976, SEQ ID NO: 226, 227) which are known.

[0055] As used herein, the term "hsa - miR - 3185 gene" or "hsa - miR - 3 185" refers to the hsa - miR - 31 85 gene (miRBase Accession No.MIMAT0015065) consisting of the nucleotide sequence represented by SEQ ID NO: 13 and also includes other species homologs or orthologs, etc. The hsa - miR - 3185 gene can be obtained by the method described in Stark MS et al., 2010, PLoS One, 5, e9685. Also, "hsa - miR - 3185" has, as its precursor, "hsa - mir - 3185" (miRBase A ccession No.MI0014227, SEQ ID NO: 228) which is known.

[0056] As used herein, the term "hsa - miR - 4746 - 3p gene" or "hsa - miR R - 4746 - 3p" refers to the hsa - miR - 4746 - 3p gene (miRBase Accession No.MIMA T0019881) consisting of the nucleotide sequence represented by SEQ ID NO: 14 and also includes other species homologs or orthologs, etc. The hsa - miR - 4746 - 3p gene is described in Persson H et al., 2011, Cancer ​It can be obtained by the method described in Res, Vol. 71, pp. 78-86. Also, " hsa-miR-4746-3p" has a hairpin-like structure as its precursor, "hsa -mir-4746" (miRBase Accession No. MI001738 5, SEQ ID NO: 229) is known.

[0057] As used herein, the term "hsa-miR-6791-5p gene" or "hsa-mi R-6791-5p" refers to the hsa-mi R-6791-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 15 (miRBase Accession No. MIMA T0027482), and also includes homologs or orthologs of other species. The hsa -miR-6791-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. Also, "hsa-miR-6791-5p" has a hairpin-like structure as its precursor "hsa-mir-6791" (miRBase Accession No. MI00 22636, SEQ ID NO: 230) is known.

[0058] As used herein, the term "hsa-miR-6893-5p gene" or "hsa-mi R-6893-5p" refers to the hsa-mi R-6893-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 16 (miRBase Accession No. MIMA T0027686), and also includes homologs or orthologs of other species. The hsa -miR-6893-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6893-5p" has a hairpin-like structure as its precursor. "hsa-mir-6893" (miRBase Accession No. MI00 22740, SEQ ID NO: 231) is known.

[0059] As used herein, "hsa-miR-4433b-3p gene" or "hsa-m The term "iR-4433b-3p" refers to a hs sequence consisting of the nucleotide sequence represented by SEQ ID NO: 17. a-miR-4433b-3p gene (miRBase Accession No. IMAT0030414) and other species homologs or orthologs. The hsa-miR-4433b-3p gene was identified in Ple H et al., 2012, PLoS O NE, Vol. 7, E50746. -miR-4433b-3p has a hairpin-like structure as its precursor, "hsa-m ir-4433b” (miRBase Accession No. MI0025511 , SEQ ID NO: 232) is known.

[0060] As used herein, "hsa-miR-3135b gene" or "hsa-miR- The term "hsa-miR-3135b" refers to the hsa-miR-3135b consisting of the nucleotide sequence represented by SEQ ID NO: 18. 3135b gene (miRBase Accession No. MIMAT00189 85) and other species homologs or orthologs. The 135b gene is described in Jima DD et al., 2010, Blood, Vol. 116, e118- The miR-313 can be obtained by the method described in e127. 5b" has a hairpin-like structure as its precursor, "hsa-mir-3135b" (m iRBase Accession No. MI0016809, sequence number 233) It is being done.

[0061] As used herein, "hsa-miR-6781-5p gene" or "hsa-mi The term "R-6781-5p" refers to an hsa- miR-6781-5p gene (miRBase Accession No. MIMA T0027462) and other species homologs or orthologs. -miR-6781-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6781-5p" has a hairpin-like structure as its precursor. "hsa-mir-6781" (miRBase Accession No. MI00 22626, SEQ ID NO: 234) is known.

[0062] As used herein, "hsa-miR-1908-5p gene" or "hsa-mi The term "R-1908-5p" refers to an hsa- miR-1908-5p gene (miRBase Accession No. MIMA T0007881) and other species homologs or orthologs. -miR-1908-5p gene is Bar M et al., 2008, Stem Cells It can be obtained by the method described in Vol. 26, pp. 2496-2505. hsa-miR-1908-5p is a precursor of hsa -mir-1908 (miRBase Accession No. MI000832 9, SEQ ID NO: 235) is known.

[0063] As used herein, the term "hsa-miR-4792 gene" or "hsa-miR-4 792" refers to the hsa-miR-47 92 gene (miRBase Accession No. MIMAT0019964) consisting of the nucleotide sequence represented by SEQ ID NO: 21 and also includes other species homologs or orthologs, etc. The hsa-miR-4792 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78 -86. Also, "hsa-miR-4792 " has, as its precursor, "hsa-mir-4792" which takes a hairpin-like structure (miRB ase Accession No. MI0017439, SEQ ID NO: 236) is known to be.

[0064] As used herein, the term "hsa-miR-7845-5p gene" or "hsa-mi R-7845-5p" refers to the hsa- miR-7845-5p gene (miRBase Accession No. MIMA T0030420) consisting of the nucleotide sequence represented by SEQ ID NO: 22 and also includes other species homologs or orthologs, etc. The hsa -miR-7845-5p gene can be obtained by the method described in Ple H et al., 2012, PLoS One, 7 e50746. Also, "hsa-miR -7845-5p" has, as its precursor, "hsa-mir-78 45" (miRBase Accession No. MI0025515, SEQ ID NO: 2 is known as 37).

[0065] As used herein, the term "hsa-miR-4417 gene" or "hsa-miR-4 417" refers to the hsa-miR-4417 gene (miRBase Accession No. MIMAT0018929) consisting of the nucleotide sequence represented by SEQ ID NO: 23 and includes other species homologs or orthologs, etc. The hsa-miR-4417 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127 . Also, "hsa-miR-4417" refers to "hsa-mir-4417" (miRBase Accession No. MI0016753, SEQ ID NO: 238) which has a hairpin-like structure as its precursor and is known.

[0066] As used herein, the term "hsa-miR-3184-5p gene" or "hsa-mi R-3184-5p" refers to the hsa-miR-3184-5p gene (miRBase Accession No. MIMA T0015064) consisting of the nucleotide sequence represented by SEQ ID NO: 24 and includes other species homologs or orthologs, etc. The hsa -miR-3184-5p gene can be obtained by the method described in Stark MS et al., 2010, PLoS One , 5, e9685. Also, "hsa-miR-3184-5p" refers to "hsa-mir- 3184" (miRBase Accession No. MI0014226, SEQ ID NO No: 239) which has a hairpin-like structure as its precursor and is known.

[0067] ​​As used herein, "hsa-miR-1225-5p gene" or "hsa-mi The term "R-1225-5p" refers to an hsa- miR-1225-5p gene (miRBase Accession No. MIMA T0005572) and other species homologs or orthologs. -miR-1225-5p gene, Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1225-5p" has a hairpin-like structure as its precursor, "h sa-mir-1225” (miRBase Accession No. MI0006 311, SEQ ID NO: 240) is known.

[0068] As used herein, "hsa-miR-1231 gene" or "hsa-miR-1 The term "miR-12" refers to the hsa-miR-12 gene consisting of the nucleotide sequence represented by SEQ ID NO: 26. 31 genes (miRBase Accession No. MIMAT0005586) hsa-miR-1231 and other species homologs or orthologs. Gene: Berezikov E et al., 2007, Mol Cell, vol. 28, p32 The miR-12 can be obtained by the method described in U.S. Pat. No. 8,336,846. 31” has a hairpin-like structure as its precursor, “hsa-mir-1231” (mi RBase Accession No. MI0006321, sequence number 241) is known. It is being done.

[0069] As used herein, "hsa-miR-1225-3p gene" or "hsa-mi The term "R-1225-3p" refers to an hsa- miR-1225-3p gene (miRBase Accession No. MIMA T0005573) and other species homologs or orthologs. -miR-1225-3p gene is Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. The precursor of "hsa-miR-1225-3p" is "hsa-miR-1225-3p", which has a hairpin-like structure. sa-mir-1225” (miRBase Accession No. MI0006 311, SEQ ID NO: 240) is known.

[0070] As used herein, "hsa-miR-150-3p gene" or "hsa-miR The term "hsa-mi-150-3p" refers to the hsa-mi-150-3p consisting of the base sequence represented by SEQ ID NO: 28. R-150-3p gene (miRBase Accession No. MIMAT00 04610) and other species homologs or orthologs. The R-150-3p gene is described in Lagos-Quintana M et al., 2002, Cur It can be obtained by the method described in rBiol, vol. 12, pp. 735-739. In addition, "hsa-miR-150-3p" has a hairpin-like structure as its precursor " hsa-mir-150” (miRBase Accession No. MI0000 479, SEQ ID NO: 242) is known.

[0071] As used herein, "hsa-miR-4433-3p gene" or "hsa-mi The term "R-4433-3p" includes the hsa-miR-4433-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 29 (miRBase Accession No. MIMAT0018949), homologs or orthologs of other species, etc. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure. The term "hsa-miR-4433-3p gene" includes the hsa-miR-4433-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 29 (miRBase Accession No. MIMAT0018949), homologs or orthologs of other species, etc. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure. The hsa-miR-4433-3p gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127. Also, "hsa-miR-4433-3p" has as its precursor "hsa-mir-4433" (miRBase Accession No. MI0016773, SEQ ID NO: 243) which takes a hairpin-like structure.

[0072] The term "hsa-miR-6125 gene" or "hsa-miR-6125" as used herein includes the hsa-miR-6125 gene consisting of the nucleotide sequence represented by SEQ ID NO: 30 (miRBase Accession No. MIMAT0024598), homologs or orthologs of other species, etc. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure. The term "hsa-miR-6125 gene" or "hsa-miR-6125" as used herein includes the hsa-miR-6125 gene consisting of the nucleotide sequence represented by SEQ ID NO: 30 (miRBase Accession No. MIMAT0024598), homologs or orthologs of other species, etc. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure. The hsa-miR-6125 gene can be obtained by the method described in Smith JL et al., 2012, J Virol, 86, p5278-5287. Also, "hsa-miR-6125" has as its precursor "hsa-mir-6125" (miRBase Accession No. MI0021259, SEQ ID NO: 244) which takes a hairpin-like structure.

[0073] The term "hsa-miR-4513 gene" or "hsa-miR-4513" as used herein includes the hsa-miR-4513 gene consisting of the nucleotide sequence represented by SEQ ID NO: 31 The term "hsa-miR-4513 gene" or "hsa-miR-4513" as used herein includes the hsa-miR-4513 gene consisting of the nucleotide sequence represented by SEQ ID NO: 31 13 gene (miRBase Accession No. MIMAT0019050) and other species homologs or orthologs, etc. are included. hsa-miR-4513 The gene can be obtained by the method described by Jima DD et al., 2010, Blood, Vol. 116, e118 - e127 Also, "hsa-miR-4513" is "hsa-mir-4513" (miRBase Accession No. MI0016879, SEQ ID NO: 245), which takes a hairpin-like structure as its precursor, is known.

[0074] As used herein, the term "hsa-miR-6787-5p gene" or "hsa-mi R-6787-5p" refers to the hsa-miR-6787-5p gene (miRBase Accession No. MIMA T0027474) consisting of the nucleotide sequence represented by SEQ ID NO: 32 and other species homologs or orthologs, etc. are included. hsa -miR-6787-5p gene can be obtained by the method described by Ladewig E et al., 2012, Genome Res, Vol. 22, p1634 - 1645. Also, "hsa-miR-6787-5p" is known to take a hairpin-like structure as its precursor "hsa-mir-6787" (miRBase Accession No. MI00 22632, SEQ ID NO: 246).

[0075] As used herein, the term "hsa-miR-6784-5p gene" or "hsa-mi R-6784-5p" refers to the hsa-miR-6784-5p gene (miRBase Accession No. MIMA consisting of the nucleotide sequence represented by SEQ ID NO: 33 including T0027468) and other species homologs or orthologs, etc. hsa - The hsa-miR-6784-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6784-5p" has a hairpin-like structure as its precursor "hsa-mir-6784" (miRBase Accession No. MI00 22629, SEQ ID NO: 247) is known.

[0076] As used herein, the term "hsa-miR-615-5p gene" or "hsa-miR -615-5p" refers to the hsa-mi R-615-5p gene (miRBase Accession No. MIMAT00 04804) and other species homologs or orthologs, etc. The hsa-mi R-615-5p gene can be obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad Sci U S A, 103, p3687-3692. Also, "hsa-miR-615-5p" has a hairpin-like structure as its precursor "hsa-mir-615" (miRBase Accessi on No. MI0003628, SEQ ID NO: 248) is known.

[0077] As used herein, the term "hsa-miR-6765-3p gene" or "hsa-mi R-6765-3p" refers to the hsa- miR-6765-3p gene (miRBase Accession No. MIMA It includes T0027431), other species homologs or orthologs, etc. hsa The -miR-6765-3p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, Vol. 22, p1634 - 1645. Also, "hsa-miR-6765-3p" takes a hairpin-like structure as its precursor "hsa-mir-6765" (miRBase Accession No.MI00 22610, SEQ ID NO: 249) is known.

[0078] As used herein, the term "hsa-miR-5572 gene" or "hsa-miR-5 572" refers to the hsa-miR-55 72 gene (miRBase Accession No.MIMAT0022260) consisting of the nucleotide sequence represented by SEQ ID NO: 36 and includes other species homologs or orthologs, etc. hsa-miR-5572 The gene can be obtained by the method described in Tandon M et al., 2012, Oral Dis, Vol. 18, p127 - 1 31. Also, "hsa-miR-5572" takes a hairpin-like structure as its precursor "hsa-mir-5572" (miRBa se Accession No.MI0019117, SEQ ID NO: 250) is known to be.

[0079] As used herein, the term "hsa-miR-6842-5p gene" or "hsa-mi R-6842-5p" refers to the hsa- miR-6842-5p gene (miRBase Accession No.MIMA T0027586) and includes other species homologs or orthologs, etc. hsa - The -miR-6842-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6842-5p" takes a hairpin-like structure as its precursor "hsa-mir-6842" (miRBase Accession No.MI00 22688, SEQ ID NO: 251) is known.

[0080] As used herein, the term "hsa-miR-8063 gene" or "hsa-miR-8 063" refers to the hsa-miR-80 63 gene (miRBase Accession No.MIMAT0030990) consisting of the nucleotide sequence represented by SEQ ID NO: 38 and also includes other species homologs or orthologs, etc. The hsa-miR-8063 gene can be obtained by the method described in Wang HJ et al., 2013, Shock, 39, p480-487. Also, "hsa-miR-8063" takes a hairpin-like structure as its precursor "hsa-mir-8063" (miRBase A ccession No.MI0025899, SEQ ID NO: 252) is known.

[0081] As used herein, the term "hsa-miR-6780b-5p gene" or "hsa-m iR-6780b-5p" refers to the hsa-miR-6780b-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 39 (miRBase Accession No.M IMAT0027572) and also includes other species homologs or orthologs, etc. The hsa-miR-6780b-5p gene is described in Ladewig E et al., 2012, Ge It can be obtained by the method described in nome Res, Vol. 22, pp. 1634-1645. Also, "hsa-miR-6780b-5p" has a hairpin-like structure as its precursor, "hsa-mir-6780b" (miRBase Accession N o.MI0022681, SEQ ID NO: 253) is known.

[0082] As used herein, the term "hsa-miR-187-5p gene" or "hsa-miR -187-5p" includes the hsa-mi R-187-5p gene (miRBase Accession No. MIMAT00 04561) consisting of the nucleotide sequence represented by SEQ ID NO: 40, as well as homologs or orthologs of other species. The hsa-mi R-187-5p gene can be obtained by the method described by Lim LP et al., 2003, Science, Vol. 299, p1540. Also, "hsa-miR-18 7-5p" has a hairpin-like structure as its precursor, "hsa-mir-187" (m iRBase Accession No. MI0000274, SEQ ID NO: 254) is known to be.

[0083] As used herein, the term "hsa-miR-128-1-5p gene" or "hsa-m iR-128-1-5p" includes the hs a-miR-128-1-5p gene (miRBase Accession No. M IMAT0026477) consisting of the nucleotide sequence represented by SEQ ID NO: 41, as well as homologs or orthologs of other species. The hsa-miR-128-1-5p gene can be obtained by the method described by Lagos-Quintana M et al., 2 002, Curr Biol, Vol. 12, pp. 735-739. It can be. Also, "hsa-miR-128-1-5p" has a hairpin-like structure as its precursor, "hsa-mir-128-1" (miRBase Accession No.MI0000447, SEQ ID NO: 255). is known. on No.MI0000447, SEQ ID NO: 255) is known.

[0084] As used herein, the term "hsa-miR-6729-5p gene" or "hsa-miR-6729-5p" refers to the hsa-miR-6729-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 42 (miRBase Accession No.MIMAT0027359), as well as homologs or orthologs of other species, etc. The hsa-miR-6729-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. R-6729-5p" refers to the hsa-miR-6729-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 42 (miRBase Accession No.MIMAT0027359), as well as homologs or orthologs of other species, etc. The hsa-miR-6729-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. miR-6729-5p gene (miRBase Accession No.MIMAT0027359), as well as homologs or orthologs of other species, etc. The hsa-miR-6729-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. T0027359) and homologs or orthologs of other species, etc. The hsa-miR-6729-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. -miR-6729-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Res, 22, p1634-1645. Also, "hsa-miR-6729-5p" has a hairpin-like structure as its precursor, "hsa-mir-6729" (miRBase Accession No.MI00 22574, SEQ ID NO: 256) is known. 22574, SEQ ID NO: 256) is known.

[0085] As used herein, the term "hsa-miR-6741-5p gene" or "hsa-miR-6741-5p" refers to the hsa-miR-6741-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 43 (miRBase Accession No.MIMAT0027383), as well as homologs or orthologs of other species, etc. The hsa-miR-6741-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. R-6741-5p" refers to the hsa-miR-6741-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 43 (miRBase Accession No.MIMAT0027383), as well as homologs or orthologs of other species, etc. The hsa-miR-6741-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. miR-6741-5p gene (miRBase Accession No.MIMAT0027383), as well as homologs or orthologs of other species, etc. The hsa-miR-6741-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. T0027383) and homologs or orthologs of other species, etc. The hsa-miR-6741-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. -miR-6741-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Res, 22, p1634-1645. In addition, "hsa-miR-6741-5p" has a hairpin-like structure as its precursor. "hsa-mir-6741" (miRBase Accession No.MI00 22586, SEQ ID NO: 257) is known.

[0086] As used herein, the term "hsa-miR-6757-5p gene" or "hsa-mi R-6757-5p" refers to the hsa-miR-6757-5p gene (miRBase Accession No.MIMA T0027414) consisting of the nucleotide sequence represented by SEQ ID NO: 44, and other species homologs or orthologs, etc. The hsa -miR-6757-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. In addition, "hsa-miR-6757-5p" has a hairpin-like structure as its precursor. "hsa-mir-6757" (miRBase Accession No.MI00 22602, SEQ ID NO: 258) is known.

[0087] As used herein, the term "hsa-miR-7110-5p gene" or "hsa-mi R-7110-5p" refers to the hsa-miR-7110-5p gene (miRBase Accession No.MIMA T0028117) consisting of the nucleotide sequence represented by SEQ ID NO: 45, and other species homologs or orthologs, etc. The hsa -miR-7110-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. ​​In addition, "hsa-miR-7110-5p" has a hairpin-like structure as its precursor. "hsa-mir-7110" (miRBase Accession No.MI00 22961, SEQ ID NO: 259) is known.

[0088] As used herein, the term "hsa-miR-7975 gene" or "hsa-miR-7 975" refers to the hsa-miR-79 75 gene (miRBase Accession No.MIMAT0031178) consisting of the nucleotide sequence represented by SEQ ID NO: 46 and also includes other species homologs or orthologs, etc. The hsa-miR-7975 gene can be obtained by the method described in Velthut-Meikas A et al., 2013, Mol Endocri nol, online version. In addition, "hsa- miR-7975" has a hairpin-like structure as its precursor, "hsa-mir-79 75" (miRBase Accession No.MI0025751, SEQ ID NO: 2 60) is known.

[0089] As used herein, the term "hsa-miR-1233-5p gene" or "hsa-mi R-1233-5p" refers to the hsa- miR-1233-5p gene (miRBase Accession No.MIMA T0022943) consisting of the nucleotide sequence represented by SEQ ID NO: 47 and also includes other species homologs or orthologs, etc. The hsa -miR-1233-5p gene can be obtained by the method described in Berezikov E et al., 2007, Mol Cell, Vol. 28, p328-336. Also ,"hsa-miR-1233-5p" has a hairpin-like structure as its precursor, "h "sa-mir-1233-1, hsa-mir-1233-2" (miRBase Ac cession No. MI0006323, MI0015973, SEQ ID NO: 261, 2 62) are known.

[0090] As used herein, the term "hsa-miR-6845-5p gene" or "hsa-mi R-6845-5p" refers to the hsa-miR-6845-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 48 (miRBase Accession No. MIMA T0027590), as well as other species homologs or orthologs, etc. The hsa -miR-6845-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6845-5p" takes a hairpin-like structure as its precursor "hsa-mir-6845" (miRBase Accession No. MI00 22691, SEQ ID NO: 263) is known.

[0091] As used herein, the term "hsa-miR-3937 gene" or "hsa-miR-3 937" refers to the hsa-miR-3937 gene consisting of the nucleotide sequence represented by SEQ ID NO: 49 (miRBase Accession No. MIMAT0018352) and other species homologs or orthologs, etc. The hsa-miR-3937 gene can be obtained by the method described in Liao JY et al., 2010, PLoS One, 5, e10563. Also, "hsa-miR-3937" is its ​​​"hsa-mir-3937" (miRBase Accession No. MI0016593, SEQ ID NO: 264), which has a hairpin-like structure as a precursor, is known. ccession No.MI0016593, SEQ ID NO: 264) is known.

[0092] As used herein, the term "hsa-miR-4467 gene" or "hsa-miR-4 467" refers to the hsa-miR-44 67 gene (miRBase Accession No. MIMAT0018994) consisting of the nucleotide sequence represented by SEQ ID NO: 50 and also includes other species homologs or orthologs, etc. The hsa-miR-4467 gene can be obtained by the method described in Jima DD et al., 2010, Blood, Vol. 116, e118 - e127. Also, "hsa-miR-4467" has a hairpin-like structure as its precursor, "hsa-mir-4467" (miRBase Accession No. MI0016818, SEQ ID NO: 265), which is known. Accession No.MI0016818, SEQ ID NO: 265) is known.

[0093] As used herein, the term "hsa-miR-7109-5p gene" or "hsa-mi R-7109-5p" refers to the hsa- miR-7109-5p gene (miRBase Accession No. MIMA T0028115) consisting of the nucleotide sequence represented by SEQ ID NO: 51 and also includes other species homologs or orthologs, etc. The hsa -miR-7109-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, Vol. 22, p1634 - 1645. Also, "hsa-miR-7109-5p" has a hairpin-like structure as its precursor "hsa-mir-7109" (miRBase Accession No. MI00 22960, SEQ ID NO: 266) is known.

[0094] As used herein, the term "hsa-miR-6088 gene" or "hsa-miR-6 088" refers to the hsa-miR-6088 gene (miRBase Accession No. MIMAT0023713) consisting of the nucleotide sequence represented by SEQ ID NO: 52 and includes other species homologs or orthologs, etc. The hsa-miR-6088 gene can be obtained by the method described in Yoo JK et al., 2012, Stem Cells Dev, 21, p2 049-2057. Also, "hsa-miR -6088" refers to "hsa-mir-6088" that forms a hairpin-like structure as its precursor (miRBase Accession No. MI0020365, SEQ ID NO: 267) is known.

[0095] As used herein, the term "hsa-miR-6782-5p gene" or "hsa-mi R-6782-5p" refers to the hsa-miR-6782-5p gene (miRBase Accession No. MIMA T0027464) consisting of the nucleotide sequence represented by SEQ ID NO: 53 and includes other species homologs or orthologs, etc. Hsa -miR-6782-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6782-5p" refers to "hsa-mir-6782" that forms a hairpin-like structure as its precursor (miRBase Accession No. MI00 22627, SEQ ID NO: 268) is known.

[0096] As used herein, the term "hsa-miR-5195-3p gene" or "hsa-mi R-5195-3p" includes the hsa-miR-5195-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 54 (miRBase Accession No. MIMA T0021127), as well as homologs or orthologs of other species. The hsa -miR-5195-3p gene can be obtained by the method described in Schotte D et al., 2011, Leukem ia, 25, p1389-1399. Also, "hsa-miR-5195-3p" has a hairpin-like structure as its precursor, "h sa-mir-5195" (miRBase Accession No. MI0018 174, SEQ ID NO: 269) is known.

[0097] As used herein, the term "hsa-miR-4454 gene" or "hsa-miR-4 454" includes the hsa-miR-4454 gene consisting of the nucleotide sequence represented by SEQ ID NO: 55 (miRBase Accession No. MIMAT0018976) and homologs or orthologs of other species. The hsa-miR-4454 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e127 and "hsa-miR-4454" has a hairpin-like structure as its precursor, "hsa-mir-4454" (miRBase Accession No. MI0016800, SEQ ID NO: 270) is known.

[0098]

[0098] As used herein, the term "hsa-miR-6724-5p gene" or "hsa-mi The term "R-6724-5p" includes the hsa-miR-6724-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 56 (miRBase Accession No. MIMA T0025856), as well as homologs or orthologs of other species. The hsa -miR-6724-5p gene can be obtained by the method described in Li Y et al., 2012, Gene, Vol. 497, p 330-335. Also, "hsa-miR- 6724-5p" has, as its precursor, "hsa-mir-672 4" which takes a hairpin-like structure (miRBase Accession No. MI0022559, SEQ ID NO: 27 1).

[0099] As used herein, the term "hsa-miR-8072 gene" or "hsa-miR-8 072" includes the hsa-miR-80 72 gene consisting of the nucleotide sequence represented by SEQ ID NO: 57 (miRBase Accession No. MIMAT0030999) and homologs or orthologs of other species. The hsa-miR-8072 gene can be obtained by the method described in Wang HJ et al., 2013, Shock, Vol. 39, p480-487 . Also, "hsa-miR-8072" has, as its precursor, "hsa-mir-8072" which takes a hairpin-like structure (miRBase A ccession No. MI0025908, SEQ ID NO: 272).

[0100] As used herein, the term "hsa-miR-4516 gene" or "hsa-miR-4 516" includes the hsa-miR-45 ​​16 genes (miRBase Accession No. MIMAT0019053) and other species homologs or orthologs, etc. are included. hsa-miR-4516 The gene can be obtained by the method described by Jima DD et al., 2010, Blood, Vol. 116, e118 - e127 Also, "hsa-miR-4516" is "hsa-mir-4516" (miRBase Accession No. MI0016882, SEQ ID NO: 273) which takes a hairpin-like structure as its precursor is known.

[0101] As used herein, the term "hsa-miR-6756-5p gene" or "hsa-mi R-6756-5p" refers to the hsa-miR-6756-5p gene (miRBase Accession No. MIMA T0027412) consisting of the nucleotide sequence represented by SEQ ID NO: 59 and other species homologs or orthologs, etc. are included. hsa -miR-6756-5p gene can be obtained by the method described by Ladewig E et al., 2012, Genome Res, Vol. 22, p1634 - 1645. Also, "hsa-miR-6756-5p" is a hairpin-like structure as its precursor "hsa-mir-6756" (miRBase Accession No. MI00 22601, SEQ ID NO: 274) is known.

[0102] As used herein, the term "hsa-miR-4665-3p gene" or "hsa-mi R-4665-3p" refers to the hsa-miR-4665-3p gene (miRBase Accession No. MIMA consisting of the nucleotide sequence represented by SEQ ID NO: 60 ​It includes T0019740), other species homologs or orthologs, etc. hsa - The hsa-miR-4665-3p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78 - 86. Also, " hsa-miR-4665-3p" has a hairpin-like structure as its precursor, "hsa -mir-4665" (miRBase Accession No.MI001729 5, SEQ ID NO: 275) is known.

[0103] As used herein, the term "hsa-miR-6826-5p gene" or "hsa-mi R-6826-5p" refers to the hsa-miR-6826-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 61 (miRBase Accession No.MIMA T0027552) and includes other species homologs or orthologs, etc. hsa - The hsa-miR-6826-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634 - 1645. Also, "hsa-miR-6826-5p" has a hairpin-like structure as its precursor "hsa-mir-6826" (miRBase Accession No.MI00 22671, SEQ ID NO: 276) is known.

[0104] As used herein, the term "hsa-miR-6820-5p gene" or "hsa-mi R-6820-5p" refers to the hsa-miR-6820-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 62 (miRBase Accession No.MIMA ​​T0027540) and other species homologs or orthologs, etc. are included. hsa - The hsa-miR-6820-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634 - 1645. Also, "hsa-miR-6820-5p" takes a hairpin-like structure as its precursor "hsa-mir-6820" (miRBase Accession No.MI00 22665, SEQ ID NO: 277) is known.

[0105] As used herein, the term "hsa-miR-6887-5p gene" or "hsa-mi R-6887-5p" refers to the hsa-miR-6887-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 63 (miRBase Accession No.MIMA T0027674) and other species homologs or orthologs, etc. are included. hsa - The hsa-miR-6887-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634 - 1645. Also, "hsa-miR-6887-5p" takes a hairpin-like structure as its precursor "hsa-mir-6887" (miRBase Accession No.MI00 22734, SEQ ID NO: 278) is known.

[0106] As used herein, the term "hsa-miR-3679-5p gene" or "hsa-mi R-3679-5p" refers to the hsa-miR-3679-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 64 (miRBase Accession No.MIMA ​​Includes T0018104), other species homologs or orthologs, etc. hsa - The miR-3679-5p gene can be obtained by the method described in Creighton CJ et al., 2010, PLoS One, Volume 5, e9637. Also, "h sa-miR-3679-5p" has a hairpin-like structure as its precursor, "hsa- mir-3679" (miRBase Accession No. MI0016080 , SEQ ID NO: 279) is known.

[0107] As used herein, the term "hsa-miR-7847-3p gene" or "hsa-mi R-7847-3p" refers to the hsa-miR-7847-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 65 (miRBase Accession No. MIMA T0030422) and includes other species homologs or orthologs, etc. hsa - The miR-7847-3p gene can be obtained by the method described in Ple H et al., 2012, PLoS One, 7 Volume, e50746. Also, "hsa-miR -7847-3p" has a hairpin-like structure as its precursor, "hsa-mir-78 47" (miRBase Accession No. MI0025517, SEQ ID NO: 2 80) is known.

[0108] As used herein, the term "hsa-miR-6721-5p gene" or "hsa-mi R-6721-5p" refers to the hsa-miR-6721-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 66 (miRBase Accession No. MIMA T0025852) and includes other species homologs or orthologs, etc. hsa ​​ - The -miR-6721-5p gene can be obtained by the method described in Li Y et al., 2012, Gene, Vol. 497, p 330-335. Also, "hsa-miR- 6721-5p" has a hairpin-like structure as its precursor, "hsa-mir-672 1" (miRBase Accession No. MI0022556, SEQ ID NO: 28 1) is known.

[0109] As used herein, the term "hsa-miR-3622a-5p gene" or "hsa-m iR-3622a-5p" includes the hsa-miR-3622a-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 67 (miRBase Accession No. M IMAT0018003), as well as homologs or orthologs of other species. The hsa-miR-3622a-5p gene can be obtained by the method described in Witten D et al., 2010, BMC Biol, Vol. 8, p58. Also, "hsa -miR-3622a-5p" has a hairpin-like structure as its precursor, "hsa-m ir-3622a" (miRBase Accession No. MI0016013 SEQ ID NO: 282) is known.

[0110] As used herein, the term "hsa-miR-939-5p gene" or "hsa-miR -939-5p" includes the hsa-miR-939-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 68 (miRBase Accession No. MIMAT00 04982), as well as homologs or orthologs of other species. The hsa-mi R-939-5p gene is described in Lui WO et al., 2007, Cancer Res, 67 ​ It can be obtained by the method described in Volume, pages 6031 - 6043. Also, "hsa" -miR-939-5p" has a hairpin-like structure as its precursor, "hsa-mir" -939" (miRBase Accession No. MI0005761, SEQ ID NO. 283) is known.

[0111] As used herein, the term "hsa-miR-602 gene" or "hsa-miR-60 2" includes the hsa-miR-602 gene (miRBase Accession No. MIMAT0003270) consisting of the nucleotide sequence represented by SEQ ID NO. 69 and its homologs or orthologs in other species. The hsa-miR-602 gene can be obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad Sci U S A, 103, pages 3687 - 3692. Also, "hsa-miR-602" has a hairpin-like structure as its precursor, "hsa" -mir-602" (miRBase Accession No. MI0003615 , SEQ ID NO. 284) is known.

[0112] As used herein, the term "hsa-miR-7977 gene" or "hsa-miR-7 977" includes the hsa-miR-79 77 gene (miRBase Accession No. MIMAT0031180) consisting of the nucleotide sequence represented by SEQ ID NO. 70 and its homologs or orthologs in other species. The hsa-miR-7977 gene is described in Velthut-Meikas A et al., 2013, Mol Endocri It can be obtained by the method described in the nol, online version. Also, "hsa- miR-7977" has a hairpin-like structure as its precursor, "hsa-mir-79 77" (miRBase Accession No. MI0025753, SEQ ID NO: 2 85) is known.

[0113] As used herein, the term "hsa-miR-6749-5p gene" or "hsa-mi R-6749-5p" refers to the hsa-miR-6749-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 71 (miRBase Accession No. MIMA T0027398), as well as other species homologs or orthologs, etc. The hsa -miR-6749-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6749-5p" has a hairpin-like structure as its precursor, "hsa-mir-6749" (miRBase Accession No. MI00 22594, SEQ ID NO: 286) is known.

[0114] As used herein, the term "hsa-miR-1914-3p gene" or "hsa-mi R-1914-3p" refers to the hsa-miR-1914-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 72 (miRBase Accession No. MIMA T0007890), as well as other species homologs or orthologs, etc. The hsa -miR-1914-3p gene can be obtained by the method described in Bar M et al., 2008, Stem Cells , 26, p2496-2505. Also, " ​ "hsa-miR-1914-3p" has a hairpin-like structure as its precursor, "hsa -mir-1914" (miRBase Accession No. MI000833 5, SEQ ID NO: 287) is known.

[0115] As used herein, the term "hsa-miR-4651 gene" or "hsa-miR-4 651" refers to the hsa-miR-46 51 gene (miRBase Accession No. MIMAT0019715) consisting of the nucleotide sequence represented by SEQ ID NO: 73 and includes other species homologs or orthologs, etc. The hsa-miR-4651 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78 -86. Also, "hsa-miR-4651 " has a hairpin-like structure as its precursor, "hsa-mir-4651" (miRB ase Accession No. MI0017279, SEQ ID NO: 288) is known .

[0116] As used herein, the term "hsa-miR-4695-5p gene" or "hsa-mi R-4695-5p" refers to the hsa- miR-4695-5p gene (miRBase Accession No. MIMA T0019788) and includes other species homologs or orthologs, etc. The hsa -miR-4695-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, " hsa-miR-4695-5p" has a hairpin-like structure as its precursor, "hsa -mir-4695」(miRBase Accession No.MI001732 8, SEQ ID NO: 289) is known.

[0117] As used herein, the term "hsa-miR-6848-5p gene" or "hsa-mi R-6848-5p" refers to the hsa-miR-6848-5p gene (miRBase Accession No.MIMA T0027596) consisting of the nucleotide sequence represented by SEQ ID NO: 75, as well as homologs or orthologs of other species. The hsa -miR-6848-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6848-5p" has a hairpin-like structure as its precursor "hsa-mir-6848" (miRBase Accession No.MI00 22694, SEQ ID NO: 290) is known.

[0118] As used herein, the term "hsa-miR-1228-3p gene" or "hsa-mi R-1228-3p" refers to the hsa-miR-1228-3p gene (miRBase Accession No.MIMA T0005583) consisting of the nucleotide sequence represented by SEQ ID NO: 76, as well as homologs or orthologs of other species. The hsa -miR-1228-3p gene can be obtained by the method described in Berezikov E et al., 2007, Mol Cell, 28, p328-336. Also ,"hsa-miR-1228-3p" has a hairpin-like structure as its precursor "h "sa-mir-1228" (miRBase Accession No. MI0006 318, SEQ ID NO: 291) is known.

[0119] As used herein, the term "hsa-miR-642b-3p gene" or "hsa-mi R-642b-3p" refers to the hsa-miR-642b-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 77 (miRBase Accession No. MIMA T0018444) and includes other species homologs or orthologs, etc. The hsa -miR-642b-3p gene can be obtained by the method described in Witten D et al., 2010, BMC Bio l, 8, p58. Also, "hsa-miR -642b-3p" has a hairpin-like structure as its precursor, "hsa-mir-64 2b" (miRBase Accession No. MI0016685, SEQ ID NO: 2 92) is known. 92) is known.

[0120] As used herein, the term "hsa-miR-6746-5p gene" or "hsa-mi R-6746-5p" refers to the hsa-miR-6746-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 78 (miRBase Accession No. MIMA T0027392) and includes other species homologs or orthologs, etc. The hsa -miR-6746-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6746-5p" has a hairpin-like structure as its precursor "hsa-mir-6746" (miRBase Accession No. MI00 "hsa-mir-6746" (miRBase Accession No. MI00 22591, SEQ ID NO: 293) is known.

[0121] As used herein, the term "hsa-miR-3620-5p gene" or "hsa-mi R-3620-5p" refers to the hsa- miR-3620-5p gene (miRBase Accession No. MIMA T0022967) consisting of the nucleotide sequence represented by SEQ ID NO: 79, and other homologs or orthologs of other species, etc. are included. The hsa -miR-3620-5p gene can be obtained by the method described in Witten D et al., 2010, BMC Bio l, Vol. 8, p58. Also, "hsa-miR -3620-5p" has a hairpin-like structure as its precursor, "hsa-mir-36 20" (miRBase Accession No. MI0016011, SEQ ID NO: 2 94) is known.

[0122] As used herein, the term "hsa-miR-3131 gene" or "hsa-miR-3 131" refers to the hsa-miR-31 31 gene (miRBase Accession No. MIMAT0014996) and other homologs or orthologs of other species, etc. are included. The hsa-miR-3131 gene can be obtained by the method described in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. Also, "hsa-miR-3131" has a hairpin-like structure as its precursor, "hsa-mir-3131" (miRBase A ccession No. MI0014151, SEQ ID NO: 295) is known. ccession No. MI0014151, SEQ ID NO: 295) is known.

[0123] As used herein, the term "hsa-miR-6732-5p gene" or "hsa-mi R-6732-5p" includes the hsa-mi R-6732-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 81 (miRBase Accession No. MIMA T0027365), as well as homologs or orthologs of other species. The hsa -miR-6732-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. In addition, "hsa-miR-6732-5p" has a hairpin-like structure as its precursor "hsa-mir-6732" (miRBase Accession No. MI00 22577, SEQ ID NO: 296) is known.

[0124] As used herein, the term "hsa-miR-7113-3p gene" or "hsa-mi R-7113-3p" includes the hsa-mi R-7113-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 82 (miRBase Accession No. MIMA T0028124), as well as homologs or orthologs of other species. The hsa -miR-7113-3p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. In addition, "hsa-miR-7113-3p" has a hairpin-like structure as its precursor "hsa-mir-7113" (miRBase Accession No. MI00 22964, SEQ ID NO: 297) is known.

[0125] As used herein, the term "hsa-miR-23a-3p gene" or "hsa-mi -23a-3p" includes the hsa-mi R-23a-3p gene (miRBase Accession No. MIMAT00 00078) consisting of the nucleotide sequence represented by SEQ ID NO: 83, and other homologs or orthologs of other species. The hsa-mi R-23a-3p gene can be obtained by the method described in Lagos-Quintana M et al., 2001, Science, 294, p853-858. Also, "hsa-miR-23a-3p" has a hairpin-like structure as its precursor, "h sa-mir-23a" (miRBase Accession No. MI00000 79, SEQ ID NO: 298) is known.

[0126] As used herein, the term "hsa-miR-3154 gene" or "hsa-miR-3 154" includes the hsa-miR-31 54 gene (miRBase Accession No. MIMAT0015028) and other homologs or orthologs of other species. The hsa-miR-3154 gene can be obtained by the method described in Berezikov E et al., 2006, Genome Res, 16, p 1289-1298. Also, "hsa-mi R-3154" has a hairpin-like structure as its precursor, "hsa-mir-3154 " (miRBase Accession No. MI0014182, SEQ ID NO: 299 ) is known.

[0127] As used herein, the term "hsa-miR-4723-5p gene" or "hsa-mi The term "R-4723-5p" includes the hsa-miR-4723-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 85 (miRBase Accession No. MIMA T0019838), as well as homologs or orthologs of other species. The hsa- miR-4723-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, "hsa- miR-4723-5p" has, as its precursor, "hsa- mir-4723" (miRBase Accession No. MI001735 9, SEQ ID NO: 300) which has a hairpin-like structure.

[0128] As used herein, the term "hsa-miR-3663-3p gene" or "hsa-miR- 3663-3p" includes the hsa-miR-3663-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 86 (miRBase Accession No. MIMA T0018085), as well as homologs or orthologs of other species. The hsa- miR-3663-3p gene can be obtained by the method described in Liao JY et al., 2010, PLoS One 5, e10563. Also, "hsa-miR-3663-3p" has, as its precursor, "hsa-mir- 3663" (miRBase Accession No. MI0016064, SEQ ID NO 301) which has a hairpin-like structure.

[0129] As used herein, the term "hsa-miR-4734 gene" or "hsa-miR-4 ​​​The term "734" refers to hsa-miR-47 consisting of the nucleotide sequence represented by SEQ ID NO: 87 gene 34 (miRBase Accession No. MIMAT0019859) and includes other species homologs or orthologs, etc. hsa-miR-4734 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78 -86. Also, "hsa-miR-4734 " has, as its precursor, "hsa-mir-4734" which takes a hairpin-like structure (miRBase Accession No. MI0017371, SEQ ID NO: 302) and is known to exist.

[0130] As used herein, the term "hsa-miR-6816-5p gene" or "hsa-mi R-6816-5p" refers to the hsa-miR-6816-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 88 (miRBase Accession No. MIMAT0027532) and includes other species homologs or orthologs, etc. hsa -miR-6816-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6816-5p" has, as its precursor, a hairpin-like structure "hsa-mir-6816" (miRBase Accession No. MI00 22661, SEQ ID NO: 303) and is known.

[0131] As used herein, the term "hsa-miR-4442 gene" or "hsa-miR-4 442" refers to hsa-miR-44 consisting of the nucleotide sequence represented by SEQ ID NO: 89 42 gene (miRBase Accession No. MIMAT0018960) and other species homologs or orthologs, etc. are included. hsa-miR-4442 The gene can be obtained by the method described by Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Also, "hsa-miR-4442" is "hsa-mir-4442" (miRBase Accession No. MI0016785, SEQ ID NO: 304) which takes a hairpin-like structure as its precursor is known.

[0132] As used herein, the term "hsa-miR-4476 gene" or "hsa-miR-4 476" refers to the hsa-miR-44 76 gene (miRBase Accession No. MIMAT0019003) and other species homologs or orthologs, etc. are included. hsa-miR-4476 The gene can be obtained by the method described by Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Also, "hsa-miR-4476" is "hsa-mir-4476" (miRBase Accession No. MI0016828, SEQ ID NO: 305) which takes a hairpin-like structure as its precursor is known.

[0133] As used herein, the term "hsa-miR-423-5p gene" or "hsa-miR -423-5p" refers to the hsa-mi R-423-5p gene (miRBase Accession No. MIMAT00 04748) and other species homologs or orthologs, etc. are included. hsa-mi The R-423-5p gene can be obtained by the method described in Kasashima K et al., 2004, Biochem Biophys Res Commun, Vol. 322, p403-410 Also, "hsa-miR-423-5p" has a precursor "hsa-mir-423" that forms a hairpin-like structure (miRBase Access ion No. MI0001445, SEQ ID NO: 306) is known.

[0134] As used herein, the term "hsa-miR-1249 gene" or "hsa-miR-1 249" refers to the hsa-miR-12 49 gene (miRBase Accession No. MIMAT0005901) consisting of the nucleotide sequence represented by SEQ ID NO: 92 and also includes other species homologs or orthologs, etc. The hsa-miR-1249 gene can be obtained by the method described in Morin RD et al., 2008, Genome Res, Vol. 18, p610 -621. Also, "hsa-miR-124 9" has a precursor "hsa-mir-1249" that forms a hairpin-like structure (miR Base Accession No. MI0006384, SEQ ID NO: 307) is known

[0135] As used herein, the term "hsa-miR-6515-3p gene" or "hsa-mi R-6515-3p" refers to the hsa- miR-6515-3p gene (miRBase Accession No. MIMA T0025487) consisting of the nucleotide sequence represented by SEQ ID NO: 93 and also includes other species homologs or orthologs, etc. The hsa -miR-6515-3p gene can be obtained by Joyce CE et al., 2011, Hum Mol​​ It can be obtained by the method described in Genet, Vol. 20, p4025 - 4040 Moreover, "hsa-miR-6515-3p" has a hairpin-like structure as its precursor, "hsa-mir-6515" (miRBase Accession No. MI 0022227, SEQ ID NO: 308) is known.

[0136] As used herein, the term "hsa-miR-887-3p gene" or "hsa-miR -887-3p" refers to the hsa-miR-887-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 94 (miRBase Accession No. MIMAT00 04951), as well as homologs or orthologs of other species. The hsa-miR -887-3p gene can be obtained by the method described in Berezikov E et al., 2006, Genome R es, Vol. 16, p1289 - 1298. Also , "hsa-miR-887-3p" has a hairpin-like structure as its precursor, "hs a-mir-887" (miRBase Accession No. MI000556 2, SEQ ID NO: 309) is known.

[0137] As used herein, the term "hsa-miR-4741 gene" or "hsa-miR-4 741" refers to the hsa-miR-47 41 gene consisting of the nucleotide sequence represented by SEQ ID NO: 95 (miRBase Accession No. MIMAT0019871) and homologs or orthologs of other species. The hsa-miR-4741 gene is described in Persson H et al., 2011, Cancer Res, Vol. 71, p78 It can be obtained by the method described in -86. Also, "hsa-miR-4741" " is known to have a hairpin-like structure as its precursor, "hsa-mir-4741" (miRB ase Accession No. MI0017379, SEQ ID NO: 310). known.

[0138] As used herein, the term "hsa-miR-6766-3p gene" or "hsa-mi R-6766-3p" refers to the hsa-miR-6766-3p gene (miRBase Accession No. MIMA T0027433) consisting of the nucleotide sequence represented by SEQ ID NO: 96, as well as other species homologs or orthologs, etc. The hsa -miR-6766-3p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6766-3p" is known to have a hairpin-like structure as its precursor, "hsa-mir-6766" (miRBase Accession No. MI00 22611, SEQ ID NO: 311). known.

[0139] As used herein, the term "hsa-miR-4673 gene" or "hsa-miR-4 673" refers to the hsa-miR-46 73 gene (miRBase Accession No. MIMAT0019755) consisting of the nucleotide sequence represented by SEQ ID NO: 97 and other species homologs or orthologs, etc. The hsa-miR-4673 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78 -86. Also, "hsa-miR-4673" " is "hsa-mir-4673" which has a hairpin-like structure as its precursor (miRB ase Accession No. MI0017304, SEQ ID NO: 312) is known to be.

[0140] As used herein, the term "hsa-miR-6779-5p gene" or "hsa-mi R-6779-5p" refers to the hsa-miR-6779-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 98 (miRBase Accession No. MIMA T0027458) and other species homologs or orthologs, etc. The hsa -miR-6779-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6779-5p" has a hairpin-like structure as its precursor "hsa-mir-6779" (miRBase Accession No. MI00 22624, SEQ ID NO: 313) is known.

[0141] As used herein, the term "hsa-miR-4706 gene" or "hsa-miR-4 706" refers to the hsa-miR-4706 gene consisting of the nucleotide sequence represented by SEQ ID NO: 99 (miRBase Accession No. MIMAT0019806) and other species homologs or orthologs, etc. The hsa-miR-4706 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78 -86. Also, "hsa-miR-4706 " has a hairpin-like structure as its precursor "hsa-mir-4706" (miRB ase Accession No. MI001995) ​ase Accession No. MI0017339, SEQ ID NO: 314) is known to be.

[0142] As used herein, the term "hsa-miR-1268b gene" or "hsa-miR- 1268b" refers to the hsa-miR -1268b gene (miRBase Accession No. MIMAT0018 925) consisting of the nucleotide sequence represented by SEQ ID NO: 100, as well as homologs or orthologs of other species. The hsa-miR- 1268b gene can be obtained by the method described in Jima DD et al., 2010, Blood, Vol. 116, e118 -e127. Also, "hsa-miR-12 68b" has, as its precursor, "hsa-mir-1268b" that takes a hairpin-like structure ( miRBase Accession No. MI0016748, SEQ ID NO: 315) is known.

[0143] As used herein, the term "hsa-miR-4632-5p gene" or "hsa-mi R-4632-5p" refers to the hsa -miR-4632-5p gene (miRBase Accession No. MIM AT0022977) consisting of the nucleotide sequence represented by SEQ ID NO: 101, as well as homologs or orthologs of other species. The hs a-miR-4632-5p gene can be obtained by the method described in Persson H et al., 2011, Cance r Res, Vol. 71, p78-86. Also, "hsa-miR-4632-5p" has, as its precursor, "hs a-mir-4632" that takes a hairpin-like structure (miRBase Accession No. MI00172 59. The one with SEQ ID NO: 316 is known.

[0144] As used herein, the term "hsa-miR-3197 gene" or "hsa-miR-3 197" refers to the hsa-miR-3 197 gene (miRBase Accession No. MIMAT0015082 ) and also includes other species homologs or orthologs, etc. The hsa-miR-319 7 gene can be obtained by the method described in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. Also, "hsa-miR-3197" has a hairpin-like structure as its precursor, "hsa-mir-3197" (miRBase Accession No. MI0014245, SEQ ID NO: 317) is known.

[0145] As used herein, the term "hsa-miR-6798-5p gene" or "hsa-mi R-6798-5p" refers to the hsa -miR-6798-5p gene (miRBase Accession No. MIM AT0027496) and also includes other species homologs or orthologs, etc. The hs a-miR-6798-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, Vol. 22, p1634 - 1645. Also, "hsa-miR-6798-5p" has a hairpin-like structure as its precursor, "hsa-mir-6798" (miRBase Accession No. MI0 022643, SEQ ID NO: 318) is known.

[0146] ​​As used herein, the term "hsa-miR-711 gene" or "hsa-miR-711" refers to the hsa-miR-711 gene consisting of the nucleotide sequence represented by SEQ ID NO: 104 (miRBase Accession No. MIMAT0012734), as well as homologs or orthologs in other species. The hsa-miR-711 gene can be obtained by the method described in Artzi S et al., 2008, BMC Bioinformatics, 9, p 39. Also, "hsa-miR-711" refers to "hsa-mir-711" which forms a hairpin-like structure as its precursor (miRBase Accession No. MI0012488, SEQ ID NO: 319).

[0147] As used herein, the term "hsa-miR-6840-3p gene" or "hsa-miR-6840-3p" refers to the hsa-miR-6840-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 105 (miRBase Accession No. MIMAT0027583), as well as homologs or orthologs in other species. The hsa-miR-6840-3p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6840-3p" refers to "hsa-mir-6840" which forms a hairpin-like structure as its precursor (miRBase Accession No. MI0022686, SEQ ID NO: 320).

[0148] As used herein, the term "hsa-miR-6763-5p gene" or "hsa-miR-6763-5p" The term "R-6763-5p" includes the hsa-miR-6763-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 106 (miRBase Accession No. MIM AT0027426), as well as homologs or orthologs of other species. The hsa-miR-6763-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22:1634-1645. Also, "hsa-miR-6763-5p" has a hairpin-like structure as its precursor, "hsa-mir-6763" (miRBase Accession No. MI0 022608, SEQ ID NO: 321), is known. The term "hsa-miR-6763-5p gene" or "hsa-miR-6763-5p" used in this specification includes the hsa-miR-6763-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 106 (miRBase Accession No. MIM AT0027426), as well as homologs or orthologs of other species. The hsa-miR-6763-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22:1634-1645. Also, "hsa-miR-6763-5p" has a hairpin-like structure as its precursor, "hsa-mir-6763" (miRBase Accession No. MI0 022608, SEQ ID NO: 321), is known. The term "hsa-miR-6727-5p gene" or "hsa-miR-6727-5p" used in this specification includes the hsa-miR-6727-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 107 (miRBase Accession No. MIM AT0027355), as well as homologs or orthologs of other species. The hsa-miR-6727-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22:1634-1645. Also, "hsa-miR-6727-5p" has a hairpin-like structure as its precursor, "hsa-mir-6727" (miRBase Accession No. MI0

[0149] The term "hsa-miR-6727-5p gene" or "hsa-miR-6727-5p" used in this specification includes the hsa-miR-6727-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 107 (miRBase Accession No. MIM AT0027355), as well as homologs or orthologs of other species. The hsa-miR-6727-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22:1634-1645. Also, "hsa-miR-6727-5p" has a hairpin-like structure as its precursor, "hsa-mir-6727" (miRBase Accession No. MI0 022572, SEQ ID NO: 322), is known. The term "hsa-miR-371a-5p gene" or "hsa-miR-371a-5p" used in this specification includes the hsa-miR-371a-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 107 (miRBase Accession No. MIM AT0027355), as well as homologs or orthologs of other species. The hsa-miR-371a-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22:1634-1645. Also, "hsa-miR-371a-5p" has a hairpin-like structure as its precursor, "hsa-mir-371a" (miRBase Accession No. MI0 022572, SEQ ID NO: 322), is known. The term "hsa-miR-371a-5p gene" or "hsa-miR-371a-5p" used in this specification includes the hsa-miR-371a-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 107 (miRBase Accession No. MIM AT0027355), as well as homologs or orthologs of other species. The hsa-miR-371a-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22:1634-1645. Also, "hsa-miR-371a-5p" has a hairpin-like structure as its precursor, "hsa-mir-371a" (miRBase Accession No. MI0 022572, SEQ ID NO: 322), is known.

[0150] The term "hsa-miR-371a-5p gene" or "hsa-miR-371a-5p" used in this specification includes the hsa-miR-371a-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 107 (miRBase Accession No. MIM The term "R-371a-5p" includes the hsa-miR-371a-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 108 (miRBase Accession No. MIM AT0004687), as well as homologs or orthologs of other species. The hsa -miR-371a-5p gene can be obtained by the method described in Suh MR et al., 2004, Dev Biol , 270, p488-498. Also, "hsa -miR-371a-5p" has a hairpin-like structure as its precursor, "hsa- mir-371a" (miRBase Accession No. MI0000779 , SEQ ID NO: 323) is known.

[0151] As used herein, the term "hsa-miR-6824-5p gene" or "hsa-mi R-6824-5p" includes the hsa-miR-6824-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 109 (miRBase Accession No. MIM AT0027548), as well as homologs or orthologs of other species. The hsa -miR-6824-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634-1645. Also, "hsa-miR-6824-5p" has a hairpin-like structure as its precursor, "hsa-mir-6824" (miRBase Accession No. MI0 022669, SEQ ID NO: 324) is known. As used herein, the term "hsa-miR-4648 gene" or "hsa-miR-4 648"

[0152] The term "648" refers to hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 110 gene 648 (miRBase Accession No. MIMAT0019710 ), and also includes homologs or orthologs of other species. hsa-miR-464 8 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p7 8-86. Also, "hsa-miR-464 8" has, as its precursor, "hsa-mir-4648" which takes a hairpin-like structure (miR Base Accession No. MI0017275, SEQ ID NO: 325) and is known .

[0153] As used herein, the term "hsa-miR-1227-5p gene" or "hsa-mi R-1227-5p" refers to hsa -miR-1227-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 111 (miRBase Accession No. MIM AT0022941), and also includes homologs or orthologs of other species. hs a-miR-1227-5p gene can be obtained by the method described in Berezikov E et al., 2007, Mol Cell, 28, p328-336. Also ,"hsa-miR-1227-5p" has, as its precursor, " hsa-mir-1227" (miRBase Accession No. MI000 6316, SEQ ID NO: 326) and is known

[0154] As used herein, the term "hsa-miR-564 gene" or "hsa-miR-56 4" refers to hsa-miR-564 consisting of the nucleotide sequence represented by SEQ ID NO: 112 Genes (miRBase Accession No. MIMAT0003228) and other homologous or orthologous genes of other species are included. The hsa-miR-564 gene can be obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad Sci U S A, 103, p3687-3692. Also, "hsa-miR-564" has a hairpin-like structure as its precursor, "hs a-mir-564" (miRBase Accession No. MI000357 0, SEQ ID NO: 327) is known. The term "hsa-miR-3679-3p gene" or "hsa-mi R-3679-3p" as used herein refers to the hsa-miR-3679-3p gene (miRBase Accession No. MIM

[0155] AT0018105) consisting of the nucleotide sequence represented by SEQ ID NO: 113 and other homologous or orthologous genes of other species. The hs a-miR-3679-3p gene can be obtained by the method described in Creighton CJ et al., 2010, PL oS One, 5, e9637. Also, " hsa-miR-3679-3p" has a hairpin-like structure as its precursor, "hsa -mir-3679" (miRBase Accession No. MI001608 0, SEQ ID NO: 279) is known. The term "hsa-miR-2861 gene" or "hsa-miR-2 861" as used herein refers to the hsa-miR-2861 gene (miRBase Accession No. MIMAT0013802 consisting of the nucleotide sequence represented by SEQ ID NO: 114.

[0156] The hsa-miR-2861 gene can be obtained by the method described in Creighton CJ et al., 2010, PL oS One, 5, e9637. ) and other species homologs or orthologs, etc. are included. hsa-miR-286 Gene 1 is described in Li H et al., 2009, J Clin Invest, 119, p36 66 - 3677 and can be obtained by the method described therein. Also, "hsa-miR- 2861" has a hairpin-like structure as its precursor, "hsa-mir-2861"( miRBase Accession No. MI0013006, SEQ ID NO: 328) is known.

[0157] As used herein, the term "hsa-miR-6737-5p gene" or "hsa-mi R-6737-5p" refers to the hsa -miR-6737-5p gene (miRBase Accession No. MIM AT0027375) consisting of the nucleotide sequence represented by SEQ ID NO: 115 and other species homologs or orthologs, etc. are included. Hs a-miR-6737-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634 - 1645 . Also, "hsa-miR-6737-5p" has a hairpin-like structure as its precursor "hsa-mir-6737" (miRBase Accession No. MI0 022582, SEQ ID NO: 329) is known.

[0158] As used herein, the term "hsa-miR-575 gene" or "hsa-miR-57 5" refers to the hsa-miR-575 gene (miRBase Accession No. MIMAT0003240) consisting of the nucleotide sequence represented by SEQ ID NO: 116 and others also includes other species homologs or orthologs, etc. The hsa-miR-575 gene is obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad Sci U S A, Vol. 103, p3687-3692 . Also, "hsa-miR-575" has a hairpin-like structure as its precursor, "hs a-mir-575" (miRBase Accession No. MI000358 2, SEQ ID NO: 330) is known

[0159] As used herein, the term "hsa-miR-4725-3p gene" or "hsa-mi R-4725-3p" includes the hsa-miR-4725-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 117 (miRBase Accession No. MIM AT0019844) and other species homologs or orthologs, etc. The hs a-miR-4725-3p gene can be obtained by the method described in Persson H et al., 2011, Cance r Res, Vol. 71, p78-86. Also, "hsa-miR-4725-3p" has a hairpin-like structure as its precursor, "hs a-mir-4725" (miRBase Accession No. MI00173 62, SEQ ID NO: 331) is known

[0160] As used herein, the term "hsa-miR-6716-5p gene" or "hsa-mi R-6716-5p" includes the hsa-miR-6716-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 118 (miRBase Accession No. MIM AT0025844) and other species homologs or orthologs, etc. Hs ​​ The a-miR-6716-5p gene can be obtained by the method described in Li Y et al., 2012, Gene, Vol. 497, pp. 330-335. Also, "hsa-miR -6716-5p" has a hairpin-like structure as its precursor, "hsa-mir-67 16" (miRBase Accession No. MI0022550, SEQ ID NO: 3 32) is known.

[0161] As used herein, the term "hsa-miR-4675 gene" or "hsa-miR-4 675" includes the hsa-miR-4 675 gene (miRBase Accession No. MIMAT0019757 ) consisting of the nucleotide sequence represented by SEQ ID NO: 119, as well as homologs or orthologs of other species. The hsa-miR-467 5 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, Vol. 71, p7 8-86. Also, "hsa-miR-467 5" has a hairpin-like structure as its precursor, "hsa-mir-4675" (miR Base Accession No. MI0017306, SEQ ID NO: 333) is known to be.

[0162] As used herein, the term "hsa-miR-1915-3p gene" or "hsa-mi R-1915-3p" includes the hsa -miR-1915-3p gene (miRBase Accession No. MIM AT0007892) consisting of the nucleotide sequence represented by SEQ ID NO: 120, as well as homologs or orthologs of other species. The hs a-miR-1915-3p gene is described in Bar M et al., 2008, Stem Cell It can be obtained by the method described in s, Volume 26, p2496-2505. Also, "hsa-miR-1915-3p" has a hairpin-like structure as its precursor, "hs a-mir-1915" (miRBase Accession No.MI00083 36, SEQ ID NO: 334) is known.

[0163] As used herein, the term "hsa-miR-671-5p gene" or "hsa-miR -671-5p" refers to the hsa-m iR-671-5p gene (miRBase Accession No.MIMAT0 003880) consisting of the nucleotide sequence represented by SEQ ID NO: 121, and also includes other species homologs or orthologs, etc. The hsa-m iR-671-5p gene can be obtained by the method described in Berezikov E et al., 2006, Genome Res, Volume 16, p1289-1298. Also, "hsa-miR-671-5p" has a hairpin-like structure as its precursor, "h sa-mir-671" (miRBase Accession No.MI00037 60, SEQ ID NO: 335) is known.

[0164] As used herein, the term "hsa-miR-3656 gene" or "hsa-miR-3 656" refers to the hsa-miR-3 656 gene (miRBase Accession No.MIMAT0018076 ) consisting of the nucleotide sequence represented by SEQ ID NO: 122, and also includes other species homologs or orthologs, etc. The hsa-miR-365 6 gene can be obtained by the method described in Meiri E et al., 2010, Nucleic Acids Res, 3 8, p6234-6246. Also, "hs a-miR-3656 has a hairpin-like structure as its precursor, hsa-mir- 3656" (miRBase Accession No. MI0016056, sequence no. No. 336) is known.

[0165] As used herein, "hsa-miR-6722-3p gene" or "hsa-mi The term "R-6722-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 123. -miR-6722-3p gene (miRBase Accession No. MIM AT0025854) and other species homologs or orthologs. The a-miR-6722-3p gene is described in Li Y et al., 2012, Gene, Vol. 497, The miR gene can be obtained by the method described on pages 330-335. -6722-3p is a precursor of hsa-mir-67, which has a hairpin-like structure. 22" (miRBase Accession No. MI0022557, SEQ ID NO: 3 37) is known.

[0166] As used herein, "hsa-miR-4707-5p gene" or "hsa-mi The term "R-4707-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 124. -miR-4707-5p gene (miRBase Accession No. MIM AT0019807) and other species homologs or orthologs. The a-miR-4707-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4707-5p" is a precursor of "hs "a-mir-4707" (miRBase Accession No. MI00173 40, SEQ ID NO: 338) is known.

[0167] As used herein, the term "hsa-miR-4449 gene" or "hsa-miR-4 449" refers to the hsa-miR-4 449 gene (miRBase Accession No. MIMAT0018968 ) and other species homologs or orthologs, etc. hsa-miR-444 9 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118 - e12 7. Also, "hsa-miR-4449" is the "hsa-mir-4449" that forms a hairpin-like structure as its precursor (miRBas e Accession No. MI0016792, SEQ ID NO: 339) is known .

[0168] As used herein, the term "hsa-miR-1202 gene" or "hsa-miR-1 202" refers to the hsa-miR-1 202 gene (miRBase Accession No. MIMAT0005865 ) and other species homologs or orthologs, etc. hsa-miR-120 2 gene can be obtained by the method described in Marton S et al., 2008, Leukemia, 22, p330 - 338. Also, "hsa-miR-1202 " is the "hsa-mir-1202" that forms a hairpin-like structure as its precursor (miRB ase Accession No. MI0006334, SEQ ID NO: 340) is known exists.

[0169] As used herein, the term "hsa-miR-4649-5p gene" or "hsa-mi R-4649-5p" includes the hsa-miR-4649-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 127 (miRBase Accession No. MIM AT0019711) and other homologs or orthologs of other species. The hsa-miR-4649-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71: 78-86. Also, "hsa-miR-4649-5p" has a hairpin-like structure as its precursor, "hs a-mir-4649" (miRBase Accession No. MI00172 76, SEQ ID NO: 341) is known.

[0170] As used herein, the term "hsa-miR-744-5p gene" or "hsa-miR -744-5p" includes the hsa-miR-744-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 128 (miRBase Accession No. MIMAT0 004945) and other homologs or orthologs of other species. The hsa-miR-744-5p gene can be obtained by the method described in Berezikov E et al., 2006, Genome Res, 16: 1289-1298. Also, "hsa-miR-744-5p" has a hairpin-like structure as its precursor, "h sa-mir-744" (miRBase Accession No. MI00055 59, SEQ ID NO: 342) is known.

[0171] ​​​​​​ As used herein, the term "hsa-miR-642a-3p gene" or "hsa-mi R-642a-3p" includes the hsa-mi R-642a-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 129 (miRBase Accession No. MIM AT0020924), as well as homologs or orthologs of other species. The hs a-miR-642a-3p gene can be obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad Sci U S A, 103, p3687-3692. Also, "hsa-miR-642a-3p" has a hairpin-like structure as its precursor, "hsa-mir-642a" (miRBase Accession No. MI0003657, SEQ ID NO: 343).

[0172] As used herein, the term "hsa-miR-451a gene" or "hsa-miR-4 51a" includes the hsa-miR-4 51a gene consisting of the nucleotide sequence represented by SEQ ID NO: 130 (miRBase Accession No. MIMAT0001631 ). Also, the hsa-miR-451 a gene can be obtained by the method described in Altuvia Y et al., 2005, Nucleic Acids Res , 33, p2697-2706. Also, " hsa-miR-451a" has a hairpin-like structure as its precursor, "hsa-mi r-451a" (miRBase Accession No. MI0001729, SEQ ID NO: 344).

[0173] ​​As used herein, the term "hsa-miR-6870-5p gene" or "hsa-mi R-6870-5p" includes the hsa-mi R-6870-5p gene (miRBase Accession No. MIM AT0027640) consisting of the nucleotide sequence represented by SEQ ID NO: 131, as well as homologs or orthologs of other species. The hs a-miR-6870-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome e Res, 22, p1634-1645 . Also, "hsa-miR-6870-5p" has a hairpin-like structure as its precursor "hsa-mir-6870" (miRBase Accession No. MI0 022717, SEQ ID NO: 345) is known.

[0174] As used herein, the term "hsa-miR-4443 gene" or "hsa-miR-4 443" includes the hsa-miR-4 443 gene (miRBase Accession No. MIMAT0018961 ) consisting of the nucleotide sequence represented by SEQ ID NO: 132, as well as homologs or orthologs of other species. The hsa-miR-444 3 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e12 7. Also, "hsa-miR-4443" has a hairpin-like structure as its precursor "hsa-mir-4443" (miRBas e Accession No. MI0016786, SEQ ID NO: 346) is known .

[0175] As used herein, the term "hsa-miR-6808-5p gene" or "hsa-mi The term "R-6808-5p" includes the hsa-miR-6808-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 133 (miRBase Accession No. MIM AT0027516), as well as homologs or orthologs of other species. The hsa-miR-6808-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634-1645. Also, "hsa-miR-6808-5p" has a hairpin-like structure as its precursor, "hsa-mir-6808" (miRBase Accession No. MI0 022653, SEQ ID NO: 347) is known. The term "hsa-miR-4728-5p gene" or "hsa-miR-4728-5p" used in this specification includes the hsa-miR-4728-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 134 (miRBase Accession No. MIM AT0019849), as well as homologs or orthologs of other species. The hsa-miR-4728-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71: p78-86. Also, "hsa-miR-4728-5p" has a hairpin-like structure as its precursor, "hsa-mir-4728" (miRBase Accession No. MI00173 65, SEQ ID NO: 348) is known. The term "hsa-miR-937-5p gene" or "hsa-miR-937-5p" used in this specification includes the hsa-miR-937-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 135 (miRBase Accession No. MIM AT0027517), as well as homologs or orthologs of other species. The hsa-miR-937-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634-1645. Also, "hsa-miR-937-5p" has a hairpin-like structure as its precursor, "hsa-mir-937" (miRBase Accession No. MI0

[0176] The term "hsa-miR-4728-5p gene" or "hsa-miR-4728-5p" used in this specification includes the hsa-miR-4728-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 134 (miRBase Accession No. MIM AT0019849), as well as homologs or orthologs of other species. The hsa-miR-4728-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71: p78-86. Also, "hsa-miR-4728-5p" has a hairpin-like structure as its precursor, "hsa-mir-4728" (miRBase Accession No. MI00173 65, SEQ ID NO: 348) is known. The term "hsa-miR-937-5p gene" or "hsa-miR-937-5p" used in this specification includes the hsa-miR-937-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 135 (miRBase Accession No. MIM AT0027517), as well as homologs or orthologs of other species. The hsa-miR-937-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634-1645. Also, "hsa-miR-937-5p" has a hairpin-like structure as its precursor, "hsa-mir-937" (miRBase Accession No. MI0 022654, SEQ ID NO: 349) is known. The term "hsa-miR-937-5p gene" or "hsa-miR-937-5p" used in this specification includes the hsa-miR-937-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 135 (miRBase Accession No. MIM AT0027517), as well as homologs or orthologs of other species. The hsa-miR-937-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634-1645. Also, "hsa-miR-937-5p" has a hairpin-like structure as its precursor, "hsa-mir-937" (miRBase Accession No. MI0 022654, SEQ ID NO: 349) is known.

[0177] The term "hsa-miR-937-5p gene" or "hsa-miR-937-5p" used in this specification includes the hsa-miR-937-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 135 (miRBase Accession No. MIM The term "-937-5p" refers to hsa-m consisting of the nucleotide sequence represented by SEQ ID NO: 135 iR-937-5p gene (miRBase Accession No. MIMAT0 022938) and other species homologs or orthologs, etc. are included. hsa-m iR-937-5p gene can be obtained by the method described in Lui WO et al., 2007, Cancer Res, 6 7, p6031-6043. Also, "hs a-miR-937-5p" has a hairpin-like structure as its precursor, "hsa-mi r-937" (miRBase Accession No. MI0005759, sequence No. 349) is known.

[0178] As used herein, the term "hsa-miR-135a-3p gene" or "hsa-mi R-135a-3p" refers to hsa -miR-135a-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 136 (miRBase Accession No. MIM AT0004595) and other species homologs or orthologs, etc. are included. Hs a-miR-135a-3p gene can be obtained by the method described in Lagos-Quintana M et al., 2002 Year, Curr Biol, 12, p735-739. Also, "hsa-miR-135a-3p" has a hairpin-like structure as its precursor, "hsa-mir-135a-1" (miRBase Accession No. MI0000452, SEQ ID NO: 350) is known. No.MI0000452, SEQ ID NO: 350) is known.

[0179] As used herein, the term "hsa-miR-663b gene" or "hsa-miR-6 The term "hsa-miR-663b" refers to hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 137 gene (miRBase Accession No. MIMAT0005867 ), and also includes homologs or orthologs of other species. The hsa-miR-663 b gene can be obtained by the method described in Takada S et al., 2008, Leukemia, 22, p1274 -1278. Also, "hsa-miR-66 3b" has, as its precursor, "hsa-mir-663b" which has a hairpin-like structure (mi RBase Accession No. MI0006336, SEQ ID NO: 351) and is known .

[0180] As used herein, the term "hsa-miR-1343-5p gene" or "hsa-mi R-1343-5p" refers to hsa -miR-1343-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 138 (miRBase Accession No. MIM AT0027038), and also includes homologs or orthologs of other species. The hs a-miR-1343-5p gene can be obtained by the method described in Persson H et al., 2011, Cance r Res, 71, p78-86. Also, "hsa-miR-1343-5p" has, as its precursor, "hs a-mir-1343" which has a hairpin-like structure (miRBase Accession No. MI00173 20, SEQ ID NO: 223) and is known.

[0181] As used herein, the term "hsa-miR-6822-5p gene" or "hsa-mi R-6822-5p" refers to hsa - The -miR-6822-5p gene (miRBase Accession No. MIM AT0027544) and homologs or orthologs of other species are included. hs The a-miR-6822-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634-1645 . Also, "hsa-miR-6822-5p" has a hairpin-like structure as its precursor "hsa-mir-6822" (miRBase Accession No. MI0 022667, SEQ ID NO: 352) is known.

[0182] As used herein, the term "hsa-miR-6803-5p gene" or "hsa-mi R-6803-5p" refers to the hsa-miR-6803-5p gene (miRBase Accession No. MIM AT0027506) consisting of the nucleotide sequence represented by SEQ ID NO: 140 and homologs or orthologs of other species are included. hs The a-miR-6803-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634-1645 and homologs or orthologs of other species are included. hs . Also, "hsa-miR-6803-5p" has a hairpin-like structure as its precursor "hsa-mir-6803" (miRBase Accession No. MI0 022648, SEQ ID NO: 353) is known.

[0183] As used herein, the term "hsa-miR-6805-3p gene" or "hsa-mi R-6805-3p" refers to the hsa-miR-6805-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 141 - The gene of hsa-miR-6805-3p (miRBase Accession No. MIM AT0027511) and homologs or orthologs of other species are included. hs The gene of hsa-miR-6805-3p can be obtained by the method described in Ladewig E et al., 2012, Genome e Res, 22, p1634-1645. In addition, "hsa-miR-6805-3p" has a hairpin-like structure as its precursor "hsa-mir-6805" (miRBase Accession No. MI0 022650, SEQ ID NO: 354) is known.

[0184] As used herein, the term "hsa-miR-128-2-5p gene" or "hsa-m iR-128-2-5p" refers to the hsa-miR-128-2-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 142 (miRBase Accession No. MIMAT0031095) and homologs or orthologs of other species are included . The hsa-miR-128-2-5p gene can be obtained by the method described in Lagos-Quintana M et al., 2002, Curr Biol, 12, p735-739. In addition, "hsa-miR-128-2-5p" has a hairpin-like structure as its precursor "hsa-mir-128-2" (miRBase Access ion No. MI0000727, SEQ ID NO: 355) is known.

[0185] As used herein, the term "hsa-miR-4640-5p gene" or "hsa-mi R-4640-5p" refers to hsa consisting of the nucleotide sequence represented by SEQ ID NO: 143 - The -miR-4640-5p gene (miRBase Accession No. MIM AT0019699) and homologs or orthologs of other species are included. hs The a-miR-4640-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, "hsa-miR-4640-5p" has a hairpin-like structure as its precursor, "hs a-mir-4640" (miRBase Accession No. MI00172 67, SEQ ID NO: 356) is known.

[0186] As used herein, the term "hsa-miR-1469 gene" or "hsa-miR-1 469" refers to the hsa-miR-1 469 gene consisting of the nucleotide sequence represented by SEQ ID NO: 144 (miRBase Accession No. MIMAT0007347 ) and homologs or orthologs of other species are included. hsa-miR-146 9 gene can be obtained by the method described in Kawaji H et al., 2008, BMC Genomics, 9, p1 57. Also, "hsa-miR-1469" has a hairpin-like structure as its precursor, "hsa-mir-1469" (miRBa se Accession No. MI0007074, SEQ ID NO: 357) is known .

[0187] As used herein, the term "hsa-miR-92a-2-5p gene" or "hsa-m iR-92a-2-5p" refers to the h sa-miR-92a-2-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 145 (miRBase Accession No. including MIMAT0004508) and other homologs or orthologs of other species 。The hsa-miR-92a-2-5p gene can be obtained by the method described by Mourelatos Z et al., 2002 year, Genes Dev, Vol. 16, p720-728. Also, "hsa-miR-92a-2-5p" has a hairpin -like structure as its precursor, "hsa-mir-92a-2" (miRBase Accession No. MI0000094, SEQ ID NO: 358) is known 。

[0188] As used herein, the term "hsa-miR-3940-5p gene" or "hsa-mi R-3940-5p" refers to the hsa-miR-3940-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 146 (miRBase Accession No. MIMAT0019229) and other homologs or orthologs of other species. The hsa a-miR-3940-5p gene can be obtained by the method described by Liao JY et al., 2010, PLoS On e, Vol. 5, e10563. Also, "hsa- miR-3940-5p" has a hairpin-like structure as its precursor, "hsa-mir -3940" (miRBase Accession No. MI0016597, sequence No. 359) is known 。

[0189] As used herein, the term "hsa-miR-4281 gene" or "hsa-miR-4 281" refers to the hsa-miR-4281 gene consisting of the nucleotide sequence represented by SEQ ID NO: 147 (miRBase Accession No. MIMAT0016907 ​) and other species homologs or orthologs, etc. are included. hsa-miR-428 1 gene can be obtained by the method described by Goff LA et al., 2009, PLoS One, Vol. 4, e7192. Also, "hsa-miR-4281" has a hairpin-like structure as its precursor, "hsa-mir-4281" (miRBase A ccession No. MI0015885, SEQ ID NO: 360) is known. The term "hsa-miR-1260b gene" or "hsa-miR- 1260b" used in this specification includes the hsa-miR

[0190] -1260b gene consisting of the nucleotide sequence represented by SEQ ID NO: 148 (miRBase Accession No. MIMAT0015 041) and other species homologs or orthologs, etc. The hsa-miR- 1260b gene can be obtained by the method described by Stark MS et al., 2010, PLoS One, Vol. 5, e9 685. Also, "hsa-miR-1260 b" has a hairpin-like structure as its precursor, "hsa-mir-1260b" (mi RBase Accession No. MI0014197, SEQ ID NO: 361) is known

[0191] . The term "hsa-miR-4758-5p gene" or "hsa-mi R-4758-5p" used in this specification includes the hsa -miR-4758-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 149 (miRBase Accession No. MIM AT0019903) and other species homologs or orthologs, etc. hs The a-miR-4758-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, "hsa-miR-4758-5p" has a hairpin-like structure as its precursor, "hs a-mir-4758" (miRBase Accession No. MI00173 99, SEQ ID NO: 362) is known.

[0192] As used herein, the term "hsa-miR-1915-5p gene" or "hsa-mi R-1915-5p" includes the hsa -miR-1915-5p gene (miRBase Accession No. MIM AT0007891) consisting of the nucleotide sequence represented by SEQ ID NO: 150, as well as homologs or orthologs of other species. hs The hsa-miR-1915-5p gene can be obtained by the method described in Bar M et al., 2008, Stem Cells, 26, p2496-2505. Also, "hsa-miR-1915-5p" has a hairpin-like structure as its precursor, "hs a-mir-1915" (miRBase Accession No. MI00083 36, SEQ ID NO: 334) is known.

[0193] As used herein, the term "hsa-miR-5001-5p gene" or "hsa-mi R-5001-5p" includes the hsa -miR-5001-5p gene (miRBase Accession No. MIM AT0021021) consisting of the nucleotide sequence represented by SEQ ID NO: 151, as well as homologs or orthologs of other species. hs ​​The a-miR-5001-5p gene can be obtained by the method described by Hansen TB et al., 2011, RNA Biol, 8, p378-383. Also, "hsa-miR-5001-5p" has a hairpin-like structure as its precursor, "hsa-mir-5001" (miRBase Accession No. MI0017867, SEQ ID NO: 363) is known. -mir-5001" (miRBase Accession No. MI001786 7, SEQ ID NO: 363) is known.

[0194] As used herein, the term "hsa-miR-4286 gene" or "hsa-miR-4286" refers to the hsa-miR-4286 gene consisting of the nucleotide sequence represented by SEQ ID NO: 152 (miRBase Accession No. MIMAT0016916 ) and also includes other species homologs or orthologs, etc. The hsa-miR-4286 gene can be obtained by the method described by Goff LA et al., 2009, PLoS One, 4, e7192. Also, "hsa-miR-4286" has a hairpin-like structure as its precursor, "hsa-mir-4286" (miRBase Accession No. MI0015894, SEQ ID NO: 364) is known. precursor, "hsa-mir-4286" (miRBase A ccession No. MI0015894, SEQ ID NO: 364) is known.

[0195] As used herein, the term "hsa-miR-6126 gene" or "hsa-miR-6126" refers to the hsa-miR-6126 gene consisting of the nucleotide sequence represented by SEQ ID NO: 153 (miRBase Accession No. MIMAT0024599 ) and also includes other species homologs or orthologs, etc. The hsa-miR-6126 gene can be obtained by the method described by Smith JL et al., 2012, J Virol, 86, p5278- ​​​​​​​​​It can be obtained by the method described in 5287. Also, "hsa-miR-612 6" has a hairpin-like structure as its precursor, "hsa-mir-6126" (miR Base Accession No. MI0021260, SEQ ID NO: 365) is known to be.

[0196] As used herein, the term "hsa-miR-6789-5p gene" or "hsa-mi R-6789-5p" refers to the hsa-miR-6789-5p gene (miRBase Accession No. MIM AT0027478) consisting of the nucleotide sequence represented by SEQ ID NO: 154, and also includes homologs or orthologs of other species. The hsa-miR-6789-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645 . Also, "hsa-miR-6789-5p" has a hairpin-like structure as its precursor, "hsa-mir-6789" (miRBase Accession No. MI0 022634, SEQ ID NO: 366) is known. . Also, "hsa-miR-6789-5p" has a hairpin-like structure as its precursor "hsa-mir-6789" (miRBase Accession No. MI0 022634, SEQ ID NO: 366) is known.

[0197] As used herein, the term "hsa-miR-4459 gene" or "hsa-miR-4 459" refers to the hsa-miR-4459 gene (miRBase Accession No. MIMAT0018981 ) consisting of the nucleotide sequence represented by SEQ ID NO: 155, and also includes homologs or orthologs of other species. The hsa-miR-4459 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e12 7. Also, "hsa-miR-4459" can be obtained by the method described. Also, "hsa-miR-4459"​​ 、 a “hsa-mir-4459” (miRBase Accession No. MI0016805, SEQ ID NO: 367) that has a hairpin-like structure as its precursor is known. .

[0198] As used herein, the term “hsa-miR-1268a gene” or “hsa-miR- 1268a” includes the hsa-miR -1268a gene (miRBase Accession No. MIMAT0005 922) consisting of the nucleotide sequence represented by SEQ ID NO: 156, as well as homologs or orthologs of other species. The hsa-miR- 1268a gene can be obtained by the method described in Morin RD et al., 2008, Genome Res, 18 , pp610-621. Also, “hsa-mi R-1268a” has a “hsa-mir-126 8a” (miRBase Accession No. MI0006405, SEQ ID NO: 3 68) that has a hairpin-like structure as its precursor is known.

[0199] As used herein, the term “hsa-miR-6752-5p gene” or “hsa-mi R-6752-5p” includes the hsa -miR-6752-5p gene (miRBase Accession No. MIM AT0027404) consisting of the nucleotide sequence represented by SEQ ID NO: 157, as well as homologs or orthologs of other species. The hs a-miR-6752-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, pp1634-1645. Also, “hsa-miR-6752-5p” has a hairpin-like structure as its precursor . The known "hsa-mir-6752" (miRBase Accession No. MI0 022597, SEQ ID NO: 369).

[0200] As used herein, the term "hsa-miR-6131 gene" or "hsa-miR-6 131" refers to the hsa-miR-6 131 gene consisting of the nucleotide sequence represented by SEQ ID NO: 158 (miRBase Accession No. MIMAT0024615 ), and also includes other species homologs or orthologs, etc. The hsa-miR-613 1 gene can be obtained by the method described in Dannemann M et al., 2012, Genome Biol Evo l, Vol. 4, p552-564. Also, "hsa-miR-6131" has a hairpin-like structure as its precursor, "hsa-mir- 6131" (miRBase Accession No. MI0021276, SEQ ID No: 370) is known.

[0201] As used herein, the term "hsa-miR-6800-5p gene" or "hsa-mi R-6800-5p" refers to the hsa -miR-6800-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 159 (miRBase Accession No. MIM AT0027500), and also includes other species homologs or orthologs, etc. The hs a-miR-6800-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, Vol. 22, p1634-1645 . Also, "hsa-miR-6800-5p" has a hairpin-like structure as its precursor, "hsa-mir-6800" (miRBase Accession No. MI0 ​​ 022645, SEQ ID NO: 371) is known.

[0202] As used herein, the term "hsa-miR-4532 gene" or "hsa-miR-4 532" encompasses the hsa-miR-4532 gene (miRBase Accession No. MIMAT0019071 ) consisting of the nucleotide sequence represented by SEQ ID NO: 160, as well as homologs or orthologs of other species. The hsa-miR-453 2 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e12 7. Also, "hsa-miR-4532" refers to "hsa-mir-4532" (miRBase Accession No. MI0016899, SEQ ID NO: 372), which forms a hairpin-like structure as its precursor, and is known . .

[0203] As used herein, the term "hsa-miR-6872-3p gene" or "hsa-mi R-6872-3p" encompasses the hsa-miR-6872-3p gene (miRBase Accession No. MIM AT0027645) consisting of the nucleotide sequence represented by SEQ ID NO: 161, as well as homologs or orthologs of other species. The hs a-miR-6872-3p gene can be obtained by the method described in Ladewig E et al., 2012, Genome e Res, 22, p1634-1645. Also, "hsa-miR-6872-3p" refers to "hsa-mir-6872" (miRBase Accession No. MI0 022719, SEQ ID NO: 373), which forms a hairpin-like structure as its precursor, and is known.

[0204] As used herein, the term "hsa-miR-718 gene" or "hsa-miR-71 8" includes the hsa-miR-718 gene (miRBase Accession No. MIMAT0012735) consisting of the nucleotide sequence represented by SEQ ID NO: 162, as well as other species homologs or orthologs thereof. The hsa-miR-718 gene can be obtained by the method described in Artzi S et al., 2008, BMC Bioinformatics, 9, p 39. Also, "hsa-miR-718" is known to have a hairpin-like structure as its precursor, "hsa-mir-718" (miRBase Accession No. MI0012489, SEQ ID NO: 374).

[0205] As used herein, the term "hsa-miR-6769a-5p gene" or "hsa-m iR-6769a-5p" includes the hsa-miR-6769a-5p gene (miRBase Accession No. MIMAT0027438) consisting of the nucleotide sequence represented by SEQ ID NO: 163, as well as other species homologs or orthologs thereof. The hsa-miR-6769a-5p gene can be obtained by the method described in Ladewig E et al., 2012, G enome Res, 22, p1634-1645. Also, "hsa-miR-6769a-5p" is known to have a hairpin -like structure as its precursor, "hsa-mir-6769a" (miRBase Accession No. MI0022614, SEQ ID NO: 375).

[0206] As used herein, the term "hsa-miR-4707-3p gene" or "hsa-mi The term "R-4707-3p" includes the hsa-miR-4707-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 164 (miRBase Accession No. MIM AT0019808) and homologs or orthologs of other species, etc. The hsa -miR-4707-3p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, "hsa-miR-4707-3p" has a hairpin-like structure as its precursor, "hs a-mir-4707" (miRBase Accession No. MI00173 40, SEQ ID NO: 338) is known.

[0207] As used herein, the term "hsa-miR-6765-5p gene" or "hsa-mi R-6765-5p" includes the hsa-miR-6765-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 165 (miRBase Accession No. MIM AT0027430) and homologs or orthologs of other species, etc. The hs a-miR-6765-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome e Res, 22, p1634-1645. Also, "hsa-miR-6765-5p" has a hairpin-like structure as its precursor, "hsa-mir-6765" (miRBase Accession No. MI0 022610, SEQ ID NO: 249) is known. 022610, SEQ ID NO: 249) is known.

[0208] As used herein, the term "hsa-miR-4739 gene" or "hsa-miR-4 The term "739" refers to hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 166 gene 739 (miRBase Accession No. MIMAT0019868 ), and also includes homologs or orthologs of other species. hsa-miR-473 gene 9 can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p7 8 - 86. Also, "hsa-miR-473 9" has, as its precursor, "hsa-mir-4739" which takes a hairpin-like structure (miR Base Accession No. MI0017377, SEQ ID NO: 376) and is known .

[0209] As used herein, the term "hsa-miR-4525 gene" or "hsa-miR-4 525" refers to hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 167 gene 525 (miRBase Accession No. MIMAT0019064 ), and also includes homologs or orthologs of other species. hsa-miR-452 gene 5 can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118 - e12 7. Also, "hsa-miR-4525" has, as its precursor, "hsa-mir-4525" which takes a hairpin-like structure (miRBas e Accession No. MI0016892, SEQ ID NO: 377) and is known .

[0210] As used herein, the term "hsa-miR-4270 gene" or "hsa-miR-4 270" refers to hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 168 270 gene (miRBase Accession No. MIMAT0016900 ) and also includes homologs or orthologs of other species, etc. hsa-miR-427 0 gene can be obtained by the method described in Goff LA et al., 2009, PLoS One, 4, e7192. Also, "hsa-miR-4270" is known to have a hairpin-like structure as its precursor, "hsa-mir-4270" (miRBase A ccession No. MI0015878, SEQ ID NO: 378).

[0211] As used herein, the term "hsa-miR-4534 gene" or "hsa-miR-4 534" refers to the hsa-miR-4 534 gene (miRBase Accession No. MIMAT0019073 ) and also includes homologs or orthologs of other species, etc. hsa-miR-453 4 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118 - e12 7. Also, "hsa-miR-4534" is known to have a hairpin-like structure as its precursor, "hsa-mir-4534" (miRBas e Accession No. MI0016901, SEQ ID NO: 379). .

[0212] As used herein, the term "hsa-miR-6785-5p gene" or "hsa-mi R-6785-5p" refers to the hsa -miR-6785-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 170 (miRBase Accession No. MIM ​It includes AT0027470), other species homologs or orthologs, etc. hs The a-miR-6785-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634 - 1645. Also, "hsa-miR-6785-5p" has a hairpin-like structure as its precursor "hsa-mir-6785" (miRBase Accession No. MI0 022630, SEQ ID NO: 380) is known.

[0213] As used herein, the term "hsa-miR-6850-5p gene" or "hsa-mi R-6850-5p" refers to the hsa-miR-6850-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 171 (miRBase Accession No. MIM AT0027600) and includes other species homologs or orthologs, etc. hs The a-miR-6850-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634 - 1645. Also, "hsa-miR-6850-5p" has a hairpin-like structure as its precursor "hsa-mir-6850" (miRBase Accession No. MI0 022696, SEQ ID NO: 381) is known.

[0214] As used herein, the term "hsa-miR-4697-5p gene" or "hsa-mi R-4697-5p" refers to the hsa-miR-4697-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 172 (miRBase Accession No. MIM AT002788) It includes AT0019791) and other biological species homologs or orthologs, etc. hs The a-miR-4697-5p gene can be obtained by the method described in Persson H et al., 2011, Cance r Res, 71, p78-86. Also, "hsa-miR-4697-5p" has a hairpin-like structure as its precursor, "hs a-mir-4697" (miRBase Accession No.MI00173 30, SEQ ID NO: 382) is known.

[0215] As used herein, the term "hsa-miR-1260a gene" or "hsa-miR- 1260a" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 173 -1260a gene (miRBase Accession No.MIMAT0005 911) and other biological species homologs or orthologs, etc. hsa-miR- The 1260a gene can be obtained by the method described in Morin RD et al., 2008, Genome Res, 18 Vol., p610-621. Also, "hsa-mi R-1260a" has a hairpin-like structure as its precursor, "hsa-mir-126 0a" (miRBase Accession No.MI0006394, SEQ ID NO: 3 83) is known.

[0216] As used herein, the term "hsa-miR-4486 gene" or "hsa-miR-4 486" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 174 486 gene (miRBase Accession No.MIMAT0019020 ) and other biological species homologs or orthologs, etc. hsa-miR-448 The 6 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e12 and can be obtained by the method described in 7. Also, "hsa-miR-4486" has a hairpin-like structure as its precursor, "hsa-mir-4486" (miRBas e Accession No. MI0016847, SEQ ID NO: 384) is known .

[0217] As used herein, the term "hsa-miR-6880-5p gene" or "hsa-mi R-6880-5p" refers to the hsa-miR-6880-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 175 (miRBase Accession No. MIM AT0027660) and other species homologs or orthologs, etc. The hsa a-miR-6880-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634-1645. Also, "hsa-miR-6880-5p" has a hairpin-like structure as its precursor "hsa-mir-6880" (miRBase Accession No. MI0 022727, SEQ ID NO: 385) is known .

[0218] As used herein, the term "hsa-miR-6802-5p gene" or "hsa-mi R-6802-5p" refers to the hsa-miR-6802-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 176 (miRBase Accession No. MIM AT0027504) and other species homologs or orthologs, etc. The hsa a-miR-6802-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22​ It can be obtained by the method described in e Res, Vol. 22, p1634-1645 In addition, "hsa-miR-6802-5p" has a hairpin-like structure as its precursor "hsa-mir-6802" (miRBase Accession No. MI0 022647, SEQ ID NO: 386) is known

[0219] As used herein, the term "hsa-miR-6861-5p gene" or "hsa-mi R-6861-5p" refers to the hsa-miR-6861-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 177 (miRBase Accession No. MIM AT0027623), as well as homologs or orthologs of other species, etc. The hsa-miR-6861-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, Vol. 22, p1634-1645 In addition, "hsa-miR-6861-5p" has a hairpin-like structure as its precursor "hsa-mir-6861" (miRBase Accession No. MI0 022708, SEQ ID NO: 387) is known As used herein, the term "hsa-miR-92b-5p gene" or "hsa-miR -92b-5p" refers to the hsa-miR-92b-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 178 (miRBase Accession No. MIMAT0 004792), as well as homologs or orthologs of other species, etc. The hsa-miR-92b-5p gene can be obtained by the method described in Cummins JM et al., 2006, Proc Nat

[0220] As used herein, the term "hsa-miR-92b-5p gene" or "hsa-miR -92b-5p" refers to the hsa-miR-92b-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 178 (miRBase Accession No. MIMAT0 004792), as well as homologs or orthologs of other species, etc. The hsa-miR-92b-5p gene can be obtained by the method described in Cummins JM et al., 2006, Proc Nat iR-92b-5p gene can be obtained by the method described in Cummins JM et al., 2006, Proc Nat The method described in l Acad Sci U S A, Vol. 103, pp. 3687-3692 can be obtained by. Also, "hsa-miR-92b-5p" and its precursor take a hairpin-like structure as "hsa-mir-92b" (miRBase Access ion No. MI0003560, SEQ ID NO: 388) is known.

[0221] As used herein, the term "hsa-miR-1238-5p gene" or "hsa-mi R-1238-5p" refers to the hsa-miR-1238-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 179 (miRBase Accession No. MIM AT0022947) and includes other species homologs or orthologs, etc. The hsa a-miR-1238-5p gene can be obtained by the method described in Berezikov E et al., 2007, Mol Cell, Vol. 28, pp. 328-336. Also, "hsa-miR-1238-5p" takes a hairpin-like structure as its precursor " hsa-mir-1238" (miRBase Accession No. MI000 6328, SEQ ID NO: 389) is known. hsa-mir-1238" (miRBase Accession No. MI000 6328, SEQ ID NO: 389) is known.

[0222] As used herein, the term "hsa-miR-6851-5p gene" or "hsa-mi R-6851-5p" refers to the hsa-miR-6851-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 180 (miRBase Accession No. MIM AT0027602) and includes other species homologs or orthologs, etc. The hsa a-miR-6851-5p gene is described in Ladewig E et al., 2012, Genom ​It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645 . Also, "hsa-miR-6851-5p" has a hairpin-like structure as its precursor "hsa-mir-6851" (miRBase Accession No. MI0 022697, SEQ ID NO: 390) is known.

[0223] As used herein, the term "hsa-miR-7704 gene" or "hsa-miR-7 704" includes the hsa-miR-7 704 gene (miRBase Accession No. MIMAT0030019 ) consisting of the nucleotide sequence represented by SEQ ID NO: 181, as well as other species homologs or orthologs, etc. The hsa-miR-770 4 gene can be obtained by the method described in Swaminathan S et al., 2013, Biochem Bioph ys Res Commun, Vol. 434, pp. 228-234. Also, "hsa-miR-7704" has a hairpin-like structure as its precursor "hsa-mir-7704" (miRBase Accession No .MI0025240, SEQ ID NO: 391) is known. .

[0224] As used herein, the term "hsa-miR-149-3p gene" or "hsa-miR -149-3p" includes the hsa-m iR-149-3p gene (miRBase Accession No. MIMAT0 004609) consisting of the nucleotide sequence represented by SEQ ID NO: 182, as well as other species homologs or orthologs, etc. The hsa-m iR-149-3p gene was described by Lagos-Quintana M et al., 2002, Cu It can be obtained by the method described in rr Biol, Vol. 12, pp. 735-739 Also, "hsa-miR-149-3p" has a hairpin-like structure as its precursor "hsa-mir-149" (miRBase Accession No. MI000 0478, SEQ ID NO: 392) is known

[0225] As used herein, the term "hsa-miR-4689 gene" or "hsa-miR-4 689" refers to the hsa-miR-4 689 gene (miRBase Accession No. MIMAT0019778 ) and includes other species homologs or orthologs, etc. The hsa-miR-468 9 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, Vol. 71, pp. 7 8-86. Also, "hsa-miR-468 9" has a hairpin-like structure as its precursor, "hsa-mir-4689" (miR Base Accession No. MI0017322, SEQ ID NO: 393) is known to be

[0226] As used herein, the term "hsa-miR-4688 gene" or "hsa-miR-4 688" refers to the hsa-miR-4 688 gene (miRBase Accession No. MIMAT0019777 ) and includes other species homologs or orthologs, etc. The hsa-miR-468 8 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, Vol. 71, pp. 7 8-86. Also, "hsa-miR-468 8” has a hairpin-like structure as its precursor, “hsa-mir-4688” (miR Base Accession No. MI0017321, SEQ ID NO: 394) is known are.

[0227] As used herein, "hsa-miR-125a-3p gene" or "hsa-mi The term "R-125a-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 185. -miR-125a-3p gene (miRBase Accession No. MIM AT0004602) and other species homologs or orthologs. The a-miR-125a-3p gene was identified by Lagos-Quintana M et al., 2002 The method described in Curr Biol, Vol. 12, p. 735-739 In addition, "hsa-miR-125a-3p" has a hairpin-like structure as its precursor. The structure of "hsa-mir-125a" (miRBase Accession No. .MI0000469, sequence number 395) is known.

[0228] As used herein, "hsa-miR-23b-3p gene" or "hsa-miR The term "HSA-M-23b-3p" refers to the HSA-M-23b-3p consisting of the base sequence represented by SEQ ID NO: 186. iR-23b-3p gene (miRBase Accession No. MIMAT0 000418) and other species homologs or orthologs. The iR-23b-3p gene is a member of the Lagos-Quintana M et al., 2002, Cu It can be obtained by the method described in rr Biol, Vol. 12, p. 735-739. In addition, "hsa-miR-23b-3p" has a hairpin-like structure as its precursor. "hsa-mir-23b" (miRBase Accession No. MI000 0439, SEQ ID NO: 396) is known.

[0229] As used herein, the term "hsa-miR-614 gene" or "hsa-miR-61 4" refers to the hsa-miR-614 gene (miRBase Accession No. MIMAT0003282) consisting of the nucleotide sequence represented by SEQ ID NO: 187, and also includes other species homologs or orthologs thereof. The hsa-miR-614 gene can be obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad Sci U S A, 103, p3687-3692. Also, "hsa-miR-614" has a hairpin-like structure as its precursor, "hs a-mir-614" (miRBase Accession No. MI000362 7, SEQ ID NO: 397) is known.

[0230] As used herein, the term "hsa-miR-1913 gene" or "hsa-miR-1 913" refers to the hsa-miR-1 913 gene (miRBase Accession No. MIMAT0007888 ) and also includes other species homologs or orthologs thereof. The hsa-miR-191 3 gene can be obtained by the method described in Bar M et al., 2008, Stem Cells, 26, p2496- 2505. Also, "hsa-miR-191 3" has a hairpin-like structure as its precursor, "hsa-mir-1913" (miR Base Accession No. MI0008334, SEQ ID NO: 398) is known to be.

[0231] As used herein, the term "hsa-miR-16-5p gene" or "hsa-miR- 16-5p" refers to the hsa-miR- 16-5p gene (miRBase Accession No. MIMAT0000 069) consisting of the nucleotide sequence represented by SEQ ID NO: 189, as well as homologs or orthologs of other species. The hsa-miR- 16-5p gene can be obtained by the method described in Lagos-Quintana M et al., 2001, Science, 294, p853-858. Also, " hsa-miR-16-5p" has, as its precursor, a hairpin-like structure, "hsa-m ir-16-1, hsa-mir-16-2" (miRBase Accession No. MI0000070, MI0000115, SEQ ID NOs: 399, 400) which are known to be.

[0232] As used herein, the term "hsa-miR-675-5p gene" or "hsa-miR -675-5p" refers to the hsa-m iR-675-5p gene (miRBase Accession No. MIMAT0 004284) consisting of the nucleotide sequence represented by SEQ ID NO: 190, as well as homologs or orthologs of other species. The hsa-m iR-675-5p gene can be obtained by the method described in Cai X et al., 2007, RNA, 13, p313- 316. Also, "hsa-miR-675- 5p" has, as its precursor, a hairpin-like structure, "hsa-mir-675" (miR ​Base Accession No. MI0005416, SEQ ID NO: 401) is known to be.

[0233] As used herein, the term "hsa-miR-486-3p gene" or "hsa-miR -486-3p" refers to the hsa-m iR-486-3p gene (miRBase Accession No. MIMAT0 004762) consisting of the nucleotide sequence represented by SEQ ID NO: 191, as well as homologs or orthologs of other species. The hsa-m iR-486-3p gene can be obtained by the method described in Fu H et al., 2005, FEBS Lett, 579 , p3849-3854. Also, "hsa- miR-486-3p" has a hairpin-like structure as its precursor, "hsa-mir- 486, hsa-mir-486-2" (miRBase Accession No. MI0002470, MI0023622, SEQ ID NO: 402, 403) is known.

[0234] As used herein, the term "hsa-miR-6777-5p gene" or "hsa-mi R-6777-5p" refers to the hsa -miR-6777-5p gene (miRBase Accession No. MIM AT0027454) consisting of the nucleotide sequence represented by SEQ ID NO: 192, as well as homologs or orthologs of other species. The hs a-miR-6777-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634-1645 . Also, "hsa-miR-6777-5p" has a hairpin-like structure as its precursor The known "hsa-mir-6777" (miRBase Accession No. MI0 022622, SEQ ID NO: 404).

[0235] As used herein, the term "hsa-miR-4497 gene" or "hsa-miR-4 497" refers to the hsa-miR-4 497 gene (miRBase Accession No. MIMAT0019032 ) and includes other species homologs or orthologs, etc. The hsa-miR-449 7 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116, e118-e12 7. Also, "hsa-miR-4497" is the "hsa-mir-4497" (miRBase Accession No. MI0016859, SEQ ID NO: 405) that forms a hairpin-like structure as its precursor, and is known .

[0236] As used herein, the term "hsa-miR-296-3p gene" or "hsa-miR -296-3p" refers to the hsa-m iR-296-3p gene (miRBase Accession No. MIMAT0 004679) and includes other species homologs or orthologs, etc. The hsa-m iR-296-3p gene can be obtained by the method described in Houbaviy HB et al., 2003, Dev Cell l, 5, p351-358. Also, "hs a-miR-296-3p" is the "hsa-mi r-296" (miRBase Accession No. MI0000747, sequence Number 406) is known.

[0237] As used herein, the term "hsa-miR-6738-5p gene" or "hsa-mi R-6738-5p" includes the hsa-miR-6738-5p gene (miRBase Accession No. MIM AT0027377) consisting of the nucleotide sequence represented by SEQ ID NO: 195, as well as homologs or orthologs of other species. The hsa-miR-6738-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. In addition, "hsa-miR-6738-5p" has a hairpin-like structure as its precursor, "hsa-mir-6738" (miRBase Accession No. MI0 022583, SEQ ID NO: 407) is known. As used herein, the term "hsa-miR-4731-5p gene" or "hsa-mi R-4731-5p" includes the hsa-miR-4731-5p gene (miRBase Accession No. MIM AT0019853) consisting of the nucleotide sequence represented by SEQ ID NO: 196, as well as homologs or orthologs of other species. The hsa-miR-4731-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. In addition, "hsa-miR-4731-5p" has a hairpin-like structure as its precursor, "hs

[0238] a-mir-4731" (miRBase Accession No. MI00173 68, SEQ ID NO: 408) is known. ​​​​​​​

[0239] As used herein, the term "hsa-miR-6889-5p gene" or "hsa-mi R-6889-5p" includes the hsa-miR-6889-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 197 (miRBase Accession No. MIM AT0027678), as well as homologs or orthologs of other species. The hsa a-miR-6889-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634-1645 . Also, "hsa-miR-6889-5p" has a hairpin-like structure as its precursor, "hsa-mir-6889" (miRBase Accession No. MI0 022736, SEQ ID NO: 409) is known .

[0240] As used herein, the term "hsa-miR-6786-5p gene" or "hsa-mi R-6786-5p" includes the hsa-miR-6786-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 198 (miRBase Accession No. MIM AT0027472), as well as homologs or orthologs of other species. The hsa a-miR-6786-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634-1645 . Also, "hsa-miR-6786-5p" has a hairpin-like structure as its precursor, "hsa-mir-6786" (miRBase Accession No. MI0 022631, SEQ ID NO: 410) is known .

[0241] ​​As used herein, the term "hsa-miR-92a-3p gene" or "hsa-miR -92a-3p" includes the hsa-miR-92a-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 199 (miRBase Accession No. MIMAT0 000092), as well as homologs or orthologs of other species. The hsa-miR-92a-3p gene can be obtained by the method described in Mourelatos Z et al., 2002, Genes Dev, 16, p720-728. Also, "hsa-miR-92a-3p" has as its precursor the hairpin-like structures "hsa -mir-92a-1, hsa-mir-92a-2" (miRBase Access ion No. MI0000093, MI0000094, SEQ ID NO: 411, 358). are known.

[0242] As used herein, the term "hsa-miR-4294 gene" or "hsa-miR-4 294" includes the hsa-miR-4294 gene consisting of the nucleotide sequence represented by SEQ ID NO: 200 (miRBase Accession No. MIMAT0016849 ) and homologs or orthologs of other species. The hsa-miR-4294 gene can be obtained by the method described in Goff LA et al., 2009, PLoS One, 4, e7192. Also, "hsa-miR-4294" has as its precursor the hairpin-like structure "hsa-mir-4294" (miRBase A ccession No. MI0015827, SEQ ID NO: 412). are known.

[0243] As used herein, the term "hsa-miR-4763-3p gene" or "hsa-mi R-4763-3p" includes the hsa-mi R-4763-3p gene (miRBase Accession No. MIM AT0019913) consisting of the nucleotide sequence represented by SEQ ID NO: 201, as well as homologs or orthologs of other species. The hs a-miR-4763-3p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, "hsa-miR-4763-3p" has a hairpin-like structure as its precursor, "hs a-mir-4763" (miRBase Accession No. MI00174 04, SEQ ID NO: 413) is known.

[0244] As used herein, the term "hsa-miR-6076 gene" or "hsa-miR-6 076" includes the hsa-miR-6 076 gene (miRBase Accession No. MIMAT0023701 ) consisting of the nucleotide sequence represented by SEQ ID NO: 202, as well as homologs or orthologs of other species. The hsa-miR-607 6 gene can be obtained by the method described in Voellenkle C et al., 2012, RNA, 18, p472-4 84. Also, "hsa-miR-6076" has a hairpin-like structure as its precursor, "hsa-mir-6076" (miRBa se Accession No. MI0020353, SEQ ID NO: 414) is known .

[0245] As used herein, the term "hsa-miR-663a gene" or "hsa-miR-6 The term "hsa-miR-663a" refers to hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 203 gene (miRBase Accession No. MIMAT0003326 ), and also includes other species homologs or orthologs, etc. The hsa-miR-663 a gene can be obtained by the method described in Cummins JM et al., 2006, Proc Natl Acad S ci U S A, Vol. 103, p3687-3692. Also, "hsa-miR-663a" has a hairpin-like structure as its precursor "hsa-mir-663a" (miRBase Accession No. MI 0003672, SEQ ID NO: 415) is known.

[0246] As used herein, the term "hsa-miR-760 gene" or "hsa-miR-76 0" refers to hsa-miR-760 consisting of the nucleotide sequence represented by SEQ ID NO: 204 gene (miRBase Accession No. MIMAT0004957) and also includes other species homologs or orthologs, etc. The hsa-miR-760 gene can be obtained by the method described in Berezikov E et al., 2006, Genome Res, Vol. 16, p128 9-1298. Also, "hsa-miR-7 60" has a hairpin-like structure as its precursor "hsa-mir-760" (miR Base Accession No. MI0005567, SEQ ID NO: 416) is known and is described.

[0247] As used herein, the term "hsa-miR-4667-5p gene" or "hsa-mi R-4667-5p" refers to hsa consisting of the nucleotide sequence represented by SEQ ID NO: 205​​ - The -miR-4667-5p gene (miRBase Accession No. MIM AT0019743) and homologs or orthologs of other species are included. hs The a-miR-4667-5p gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p78-86. Also, "hsa-miR-4667-5p" has a hairpin-like structure as its precursor, "hs a-mir-4667" (miRBase Accession No. MI00172 97, SEQ ID NO: 417) is known.

[0248] As used herein, the term "hsa-miR-6090 gene" or "hsa-miR-6 090" refers to the hsa-miR-6 gene consisting of the nucleotide sequence represented by SEQ ID NO: 206 (miRBase Accession No. MIMAT0023715 ) and homologs or orthologs of other species are included. hsa-miR-609 0 gene can be obtained by the method described in Yoo JK et al., 2012, Stem Cells Dev, 21, p 2049-2057. Also, "hsa-mi R-6090" has a hairpin-like structure as its precursor, "hsa-mir-6090 " (miRBase Accession No. MI0020367, SEQ ID NO: 418 ) is known.

[0249] As used herein, the term "hsa-miR-4730 gene" or "hsa-miR-4 730" refers to the hsa-miR-4 gene consisting of the nucleotide sequence represented by SEQ ID NO: 207 The 730 gene (miRBase Accession No. MIMAT0019852 ) and other homologs or orthologs of other species are included. hsa-miR-473 The 0 gene can be obtained by the method described in Persson H et al., 2011, Cancer Res, 71, p7 8 - 86. Also, "hsa-miR-473 0" has a hairpin-like structure as its precursor, "hsa-mir-4730" (miR Base Accession No. MI0017367, SEQ ID NO: 419) is known .

[0250] As used herein, the term "hsa-miR-7106-5p gene" or "hsa-mi R-7106-5p" refers to the hsa-miR-7106-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 208 (miRBase Accession No. MIM AT0028109) and other homologs or orthologs of other species are included. Hs a-miR-7106-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genom e Res, 22, p1634 - 1645 . Also, "hsa-miR-7106-5p" has a hairpin-like structure as its precursor . "hsa-mir-7106" (miRBase Accession No. MI0 022957, SEQ ID NO: 420) is known

[0251] As used herein, the term "hsa-miR-3196 gene" or "hsa-miR-3 196" refers to the hsa-miR-3196 gene consisting of the nucleotide sequence represented by SEQ ID NO: 209 (miRBase Accession No. MIMAT0015080 ​ ) and other species homologs or orthologs, etc. are included. hsa-miR-319 The 6 genes can be obtained by the method described by Stark MS et al., 2010, PLoS One, Vol. 5, e9685 Also, "hsa-miR-3196" has a hairpin-like structure as its precursor, "hsa-mir-3196" (miRBase Accession No. MI0014241, SEQ ID NO: 421) is known.

[0252] As used herein, the term "hsa-miR-5698 gene" or "hsa-miR-5 698" refers to the hsa-miR-5 698 gene consisting of the nucleotide sequence represented by SEQ ID NO: 210 (miRBase ) and other species homologs or orthologs, etc. are included. hsa-miR-569 8 gene can be obtained by the method described by Watahiki A et al., 2011, PLoS One, Vol. 6, e249 50. Also, "hsa-miR-5698" has a hairpin-like structure as its precursor, "hsa-mir-5698" (miRBa se Accession No. MI0019305, SEQ ID NO: 422) is known .

[0253] As used herein, the term "hsa-miR-6087 gene" or "hsa-miR-6 087" refers to the hsa-miR-6 087 gene consisting of the nucleotide sequence represented by SEQ ID NO: 211 (miRBase ) and other species homologs or orthologs, etc. are included. hsa-miR-608 ​The 7 gene can be obtained by the method described in Yoo JK et al., 2012, Stem Cells Dev, 21, p 2049-2057. Also, "hsa-mi R-6087" has a hairpin-like structure as its precursor, "hsa-mir-6087 " (miRBase Accession No. MI0020364, SEQ ID NO: 423 ).

[0254] As used herein, the term "hsa-miR-4665-5p gene" or "hsa-mi R-4665-5p" includes the hsa -miR-4665-5p gene (miRBase Accession No. MIM AT0019739) consisting of the nucleotide sequence represented by SEQ ID NO: 212, as well as homologs or orthologs of other species. The hs a-miR-4665-5p gene can be obtained by the method described in Persson H et al., 2011, Cance r Res, 71, p78-86. Also, "hsa-miR-4665-5p" has a hairpin-like structure as its precursor, "hs a-mir-4665" (miRBase Accession No. MI00172 95, SEQ ID NO: 275).

[0255] As used herein, the term "hsa-miR-8059 gene" or "hsa-miR-8 059" includes the hsa-miR-8 059 gene (miRBase Accession No. MIMAT0030986 ) consisting of the nucleotide sequence represented by SEQ ID NO: 213, as well as homologs or orthologs of other species. The hsa-miR-805 9 gene is described in Wang HJ et al., 2013, Shock, 39, p480-487 It can be obtained by the method described. Also, "hsa-miR-8059" has a hairpin-like structure as its precursor, "hsa-mir-8059" (miRBase Accession No. MI0025895, SEQ ID NO: 424) is known.

[0256] As used herein, the term "hsa-miR-6879-5p gene" or "hsa-mi R-6879-5p" refers to the hsa-miR-6879-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 214 (miRBase Accession No. MIM AT0027658), as well as homologs or orthologs of other species, etc. The hsa a-miR-6879-5p gene can be obtained by the method described by Ladewig E et al., 2012, Genome Res, 22: p1634-1645. Also, "hsa-miR-6879-5p" has a hairpin-like structure as its precursor, "hsa-mir-6879" (miRBase Accession No. MI0 022726, SEQ ID NO: 425) is known. 022726, SEQ ID NO: 425) is known.

[0257] As used herein, the term "hsa-miR-6717-5p gene" or "hsa-mi R-6717-5p" refers to the hsa-miR-6717-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 666 (miRBase Accession No. MIM AT0025846), as well as homologs or orthologs of other species, etc. are included. The h sa-miR-6717-5p gene can be obtained by the method described by Li Y et al., 2012, Gene, 497: p330-335. Also, "hsa-mi "R-6717-5p" has a hairpin-like structure as its precursor, "hsa-mir-6" 717" (miRBase Accession No. MI0022551, SEQ ID NO: 677) is known.

[0258] As used herein, the term "hsa-miR-3648 gene" or "hsa-miR-3 648" refers to the hsa-miR-3 648 gene (miRBase Accession No. MIMAT0018068 ) and other species homologs or orthologs, etc. are included. The hsa-miR-36 48 gene can be obtained by the method described in Meiri E et al., 2010, Nucleic Acids Res, 38, p6234-6246. Also, "h sa-miR-3648" has a hairpin-like structure as its precursor, "hsa-mir -3648" (miRBase Accession No. MI0016048, SEQ ID NO: 678) is known.

[0259] As used herein, the term "hsa-miR-3162-5p gene" or "hsa-mi R-3162-5p" refers to the hsa -miR-3162-5p gene (miRBase Accession No. MIM AT0015036) and other species homologs or orthologs, etc. are included. The h sa-miR-3162-5p gene can be obtained by the method described in Stark MS et al., 2010, PLoS One, 5, e9685. Also, "hsa -miR-3162-5p" has a hairpin-like structure as its precursor, "hsa-mi "r-3162" (miRBase Accession No. MI0014192, sequence number 679) is known.

[0260] As used herein, the term "hsa-miR-1909-3p gene" or "hsa-mi R-1909-3p" refers to the hsa-miR-1909-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 669 (miRBase Accession No. MIM AT0007883), as well as homologs or orthologs of other species. The h sa-miR-1909-3p gene can be obtained by the method described by Bar M et al., 2008, Stem Cel ls, 26, p2496-2505. Also, "hsa-miR-1909-3p" has a hairpin-like structure as its precursor, "h sa-mir-1909" (miRBase Accession No. MI0008 330, SEQ ID NO: 680) is known.

[0261] As used herein, the term "hsa-miR-8073 gene" or "hsa-miR-8 073" refers to the hsa-miR-8073 gene consisting of the nucleotide sequence represented by SEQ ID NO: 670 (miRBase Accession No. MIMAT0031000 ) and homologs or orthologs of other species. The hsa-miR-80 73 gene can be obtained by the method described by Wang HJ et al., 2013, Shock, 39, p480-487 . Also, "hsa-miR-8073" has a hairpin-like structure as its precursor, "hsa-mir-8073" (miRBase Accession No. MI0025909, SEQ ID NO: 681) is known. ​

[0262] As used herein, the term "hsa-miR-6769b-5p gene" or "hsa-m iR-6769b-5p" refers to the hsa-miR-6769b-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 671 (miRBase Accession No. MIMAT0027620), as well as other species homologs or orthologs, etc. are included. The hsa-miR-6769b-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6769b-5p" has a hairpin-like structure as its precursor, "hsa-mir-6769b" (miRBase Accession No. MI0022706, SEQ ID NO: 682) is known. Genome Res, 22, p1634-1645. As used herein, the term "hsa-miR-6836-3p gene" or "hsa-mi R-6836-3p" refers to the hsa-miR-6836-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 672 (miRBase Accession No. MIM AT0027575), as well as other species homologs or orthologs, etc. are included. The hsa-miR-6836-3p gene can be obtained by the method described in Ladewig E et al., 2012, Geno

[0263] me Res, 22, p1634-1645. Also, "hsa-miR-6836-3p" has a hairpin-like structure as its precursor, "hsa-mir-6836" (miRBase Accession No. MI 0022682, SEQ ID NO: 683) is known. AT0027575), as well as other species homologs or orthologs, etc. are included. The hsa-miR-6836-3p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6836-3p" has a hairpin-like structure as its precursor, "hsa-mir-6836" (miRBase Accession No. MI 0022682, SEQ ID NO: 683) is known. sa-miR-6836-3p gene can be obtained by the method described in Ladewig E et al., 2012, Geno me Res, 22, p1634-1645. Also, "hsa-miR-6836-3p" has a hairpin-like structure as its precursor, "hsa-mir-6836" (miRBase Accession No. MI 0022682, SEQ ID NO: 683) is known. 0022682, SEQ ID NO: 683) is known. 0022682, SEQ ID NO: 683) is known.

[0264] As used herein, the term "hsa-miR-4484 gene" or "hsa-miR-4 484" includes the hsa-miR-4484 gene consisting of the nucleotide sequence represented by SEQ ID NO: 673 (miRBase Accession No. MIMAT0019018 ), as well as other species homologs or orthologs. The hsa-miR-44 84 gene can be obtained by the method described in Jima DD et al., 2010, Blood, 116: e118 - e1 27. Also, "hsa-miR-4484" has a hairpin-like structure as its precursor, "hsa-mir-4484" (miRBa se Accession No. MI0016845, SEQ ID NO: 684), which is known .

[0265] As used herein, the term "hsa-miR-6819-5p gene" or "hsa-mi R-6819-5p" includes the hsa-miR-6819-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 674 (miRBase Accession No. MIM AT0027538), as well as other species homologs or orthologs. The h sa-miR-6819-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22: p1634 - 1645 . Also, "hsa-miR-6819-5p" has a hairpin-like structure as its precursor, "hsa-mir-6819" (miRBase Accession No. MI 0022664, SEQ ID NO: 685), which is known .

[0266] As used herein, the term "hsa-miR-6794-5p gene" or "hsa-mi​​​​ The term "R-6794-5p" includes the hsa-miR-6794-5p gene consisting of the nucleotide sequence represented by SEQ ID NO: 675 (miRBase Accession No. MIM AT0027488), as well as homologs or orthologs of other species. The hsa-miR-6794-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. In addition, "hsa-miR-6794-5p" has a hairpin-like structure as its precursor, "hsa-mir-6794" (miRBase Accession No. MI 0022639, SEQ ID NO: 686) is known.

[0267] As used herein, the term "hsa-miR-24-3p gene" or "hsa-miR-24-3p" includes the hsa-miR-24-3p gene consisting of the nucleotide sequence represented by SEQ ID NO: 676 (miRBase Accession No. MIMAT0000 080), as well as homologs or orthologs of other species. The hsa-miR-24-3p gene can be obtained by the method described in Lagos-Quintana M et al., 2001, Science, 294, p853-858. In addition, "hsa-miR-24-3p" has a hairpin-like structure as its precursor, "hsa-mir-24-1, hsa-mir-24-2" (miRBase Accession No. MI0000080, MI0000081, SEQ ID NO: 687, 688) is known.

[0268] In addition, mature miRNAs are derived from RNA precursors with a hairpin-like structure. When they are excised as miRNA A, one to several bases at the front and back of the sequence may be excised shorter or longer, or base substitutions may occur, resulting in mutants, which are called isomiRs (Morin R D. et al., 2008, Genome Res., Vol. 18, p. 610-621). In miRBase Release20, in addition to the base sequences represented by any of SEQ ID NOs: 1 to 214 and 666 to 676, mutants and fragments of base sequences represented by any of SEQ ID NOs: 426 to 665 and 689 to 700, which are called various isomiRs, are also shown. These mutants can also be obtained as miRNAs having the base sequences represented by any of SEQ ID NOs: 1 to 214 and 666 to 676. That is, SEQ ID NOs: 1, 2, 6, 9, 13, 18, 20, 21, 23, 28, 29, 30, 31, 34, 36, 40, 41, 46, 47, 50, 52, 54, 55, 5 6, 58, 64, 66, 67, 68, 72, 73, 74, 76, 77, 79, 80, 83 、84、85、87、89、90、91、92、93、94、95、97、99、100 、101、102、104、108、110、112、113、114、117、118 、120、121、122、124、125、126、127、128、129、130

[0269] 、132、134、135、136、137、142、143、145、146、147 、148、149、150、151、152、153、155、156、158、160 、162、164、166、167、173、174、178、182、183、184 、185、186、187、188、189、190、191、193、194、196 、197、198、200、201、202、203、205、206、208、210 、211、212、213、214、215、216、217、218、219、220 、221、222、223、224、225、226、227、228、229、230 、231、232、233、234、235、236、237、238、239、240 、241、242、243、244、245、246、247、248、249、250 、251、252、253、254、255、256、257、258、259、260 , 199, 201, 203, 204, 205, 207, 209, 210, 211, 212 , a polynucleotide variant consisting of the nucleotide sequence represented by 666, 667, 668, 669, 673, and 676, or the nucleotide sequence in which u is t in the nucleotide sequence, for example Among the variants of the polynucleotide consisting of the nucleotide sequence represented by 666, 667, 668, 669, 673, and 676, or the nucleotide sequence in which u is t in the nucleotide sequence, for example As the longest variant registered in miRBase Release 20, respectively SEQ ID NOs: 426, 428, 430, 432, 434, 436, 438, 440, 442 , 444, 446, 448, 450, 452, 454, 456, 458, 460, 462 , 464, 466, 468, 470, 472, 474, 476, 478, 480, 482 , 484, 486, 488, 490, 492, 494, 496, 498, 500, 502 , 504, 506, 508, 510, 512, 514, 516, 518, 520, 522 , 524, 526, 528, 530, 532, 534, 536, 538, 540, 542 , 544, 546, 548, 550, 552, 554, 556, 558, 560, 562 , 564, 566, 568, 570, 572, 574, 576, 578, 580, 582 , 584, 586, 588, 590, 592, 594, 596, 598, 600, 602 , 604, 606, 608, 610, 612, 614, 616, 618, 620, 622 , 624, 626, 628, 630, 632, 634, 636, 638, 640, 642 , 644, 646, 648, 650, 652, 654, 656, 658, 660, 662 , polynucleotides represented by 664, 689, 691, 693, 695, 697, and 699 are included. Also, SEQ ID NOs: 1, 2, 6, 9, 13, 18, 20, 21, of the present invention 23, 28, 29, 30, 31, 34, 36, 40, 41, 46, 47, 50, 52, 5 4, 55, 56, 58, 64, 66, 67, 68, 72, 73, 74, 76, 77, 79 , 80, 83, 84, 85, 87, 89, 90, 91, 92, 93, 94, 95, 97, 99, 100, 101, 102, 104, 108, 110, 112, 113, 114, 1 17, 118, 120, 121, 122, 124, 125, 126, 127, 128, 1 29, 130, 132, 134, 135, 136, 137, 142, 143, 145, 1 46, 147, 148, 149, 150, 151, 152, 153, 155, 156, 1 58, 160, 162, 164, 166, 167, 173, 174, 178, 182, 1 83, 184, 185, 186, 187, 188, 189, 190, 191, 193, 1 94, 196, 199, 201, 203, 204, 205, 207, 209, 210, 2 a polynucleotide consisting of the nucleotide sequences represented by 11, 212, 666, 667, 668, 669, 673 and 676 or a variant thereof consisting of a nucleotide sequence in which u is t in said nucleotide sequence Among them, for example, the shortest variant registered in miRBase Release 20 As, respectively, SEQ ID NO: 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, polynucleotides of the sequences represented by 661, 663, 665, 690, 692, 694, 696, 698 and 700 are included. In addition to these variants and fragments, polynucleotides of various isomiRs registered in miRBase, which are composed of the base sequences represented by SEQ ID NOs: 1 to 214 and 666 to 676, are included. Further, examples of polynucleotides containing the base sequence represented by any of SEQ ID NOs: 1 to 214 and 666 to 676 include polynucleotides represented by any of SEQ ID NOs: 215 to 425 and 677 to 688, which are their respective precursors. In addition to these variants and fragments, polynucleotides of various isomiRs registered in miRBase, which are composed of the base sequences represented by SEQ ID NOs: 1 to 214 and 666 to 676, are included. Further, examples of polynucleotides containing the base sequence represented by any of SEQ ID NOs: 1 to 214 and 666 to 676 include polynucleotides represented by any of SEQ ID NOs: 215 to 425 and 677 to 688, which are their respective precursors. polynucleotides of various isomiRs registered in miRBase, which are composed of the base sequences represented by SEQ ID NOs: 1 to 214 and 666 to 676, are included. In addition to these variants and fragments, polynucleotides of various isomiRs registered in miRBase, which are composed of the base sequences represented by SEQ ID NOs: 1 to 214 and 666 to 676, are included. Further, examples of polynucleotides containing the base sequence represented by any of SEQ ID NOs: 1 to 214 and 666 to 676 include polynucleotides represented by any of SEQ ID NOs: 215 to 425 and 677 to 688, which are their respective precursors. Further, examples of polynucleotides containing the base sequence represented by any of SEQ ID NOs: 1 to 214 and 666 to 676 include polynucleotides represented by any of SEQ ID NOs: 215 to 425 and 677 to 688, which are their respective precursors. include polynucleotides represented by any of SEQ ID NOs: 215 to 425 and 677 to 688, which are their respective precursors.

[0270] The names of the genes composed of the base sequences represented by SEQ ID NOs: 1 to 700 and the miRBase Accession No. (registration number) are shown in Table 1. The names of the genes composed of the base sequences represented by SEQ ID NOs: 1 to 700 and the miRBase Accession No. (registration number) are shown in Table 1.

[0271] As used herein, "specifically bindable" means that the nucleic acid probe or primer used in the present invention binds to a specific target nucleic acid and cannot substantially bind to other nucleic acids. As used herein, "specifically bindable" means that the nucleic acid probe or primer used in the present invention binds to a specific target nucleic acid and cannot substantially bind to other nucleic acids. means that the nucleic acid probe or primer used in the present invention binds to a specific target nucleic acid and cannot substantially bind to other nucleic acids.

[0272] [Table 1]

[0273] TIFF0007714199000002.tif245135

[0274] TIFF0007714199000003.tif244134

[0275] TIFF0007714199000004.tif245135

[0276] TIFF0007714199000005.tif245133

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[0280] TIFF0007714199000009.tif246135

[0281] TIFF0007714199000010.tif244134

[0282] TIFF0007714199000011.tif245135

[0283] TIFF0007714199000012.tif245136

[0284] TIFF0007714199000013.tif246134

[0285] TIFF0007714199000014.tif246136

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[0289] TIFF0007714199000018.tif245135

[0290] TIFF0007714199000019.tif245134

[0291] This specification incorporates the content described in the specifications and / or drawings of Japanese Patent Applications No. 2014-125036 and No. 2015-070379, which are the basis of the priority of this application. and / or drawings.

Advantages of the Invention

[0292] According to the present invention, it has become possible to easily and highly accurately detect esophageal cancer. For example, by using the measured values of several miRNAs in the blood, serum, and / or plasma of patients that can be collected in a minimally invasive manner as indicators, it is possible to easily detect whether a patient has esophageal cancer or not.

Brief Description of the Drawings

[0293]

Figure 1

Figure 2

Figure 3

Figure 4

Mode for Carrying Out the Invention

[0294] The present invention will be described in more detail below. 1. Target Nucleic Acid for Esophageal Cancer Using the nucleic acid probe or primer for detecting esophageal cancer as defined above of the present invention, esophageal cancer as an esophageal cancer marker for detecting the presence and / or absence of esophageal cancer or esophageal cancer cells; The main target nucleic acids of miR-204-3p and miR-1247-3 p, hsa-miR-6875-5p, hsa-miR-6857-5p, hsa-mi R-6726-5p, hsa-miR-3188, hsa-miR-8069, hsa- miR-4257, hsa-miR-1343-3p, hsa-miR-7108-5p , hsa-miR-6825-5p, hsa-miR-7641, hsa-miR-31 85, hsa-miR-4746-3p, hsa-miR-6791-5p, hsa-m iR-6893-5p, hsa-miR-4433b-3p, hsa-miR-3135 b, hsa-miR-6781-5p, hsa-miR-1908-5p, hsa-mi R-4792, hsa-miR-7845-5p, hsa-miR-4417, hsa- miR-3184-5p, hsa-miR-1225-5p, hsa-miR-1231 , hsa-miR-1225-3p, hsa-miR-150-3p, hsa-miR- 4433-3p, hsa-miR-6125, hsa-miR-4513, hsa-mi R-6787-5p, hsa-miR-6784-5p, hsa-miR-615-5p , hsa-miR-6765-3p, hsa-miR-5572, hsa-miR-68 42-5p, hsa-miR-8063, hsa-miR-6780b-5p, hsa- miR-187-5p, hsa-miR-128-1-5p, hsa-miR-6729 -5p, hsa-miR-6741-5p, hsa-miR-6757-5p, hsa- miR-7110-5p, hsa-miR-7975, hsa-miR-1233-5p , hsa-miR-6845-5p, hsa-miR-3937, hsa-miR-44 67, hsa-miR-7109-5p, hsa-miR-6088, hsa-miR- 6782-5p, hsa-miR-5195-3p, hsa-miR-4454, hsa -miR-6724-5p, hsa-miR-8072, hsa-miR-4516, h sa-miR-6756-5p, hsa-miR-4665-3p, hsa-miR-6 826-5p, hsa-miR-6820-5p, hsa-miR-6887-5p, h sa-miR-3679-5p, hsa-miR-7847-3p, hsa-miR-6 721-5p, hsa-miR-3622a-5p, hsa-miR-939-5p, h sa-miR-602, hsa-miR-7977, hsa-miR-6749-5p, hsa-miR-1914-3p, hsa-miR-4651, hsa-miR-469 5-5p, hsa-miR-6848-5p, hsa-miR-1228-3p, hsa -miR-642b-3p, hsa-miR-6746-5p, hsa-miR-362 0-5p, hsa-miR-3131, hsa-miR-6732-5p, hsa-mi R-7113-3p, hsa-miR-23a-3p, hsa-miR-3154, hs [[ID=,29]]a-miR-4723-5p, hsa-miR-3663-3p, hsa-miR-47 34, hsa-miR-6816-5p, hsa-miR-4442, hsa-miR- 4476, hsa-miR-423-5p, hsa-miR-1249, hsa-miR -6515-3p, hsa-miR-887-3p, hsa-miR-4741, hsa -miR-6766-3p, hsa-miR-4673, hsa-miR-6779-5 p, hsa-miR-4706, hsa-miR-1268b, hsa-miR-463 2-5p, hsa-miR-3197, hsa-miR-6798-5p, hsa-mi R-711, hsa-miR-6840-3p, hsa-miR-6763-5p, hs a-miR-6727-5p, hsa-miR-371a-5p, hsa-miR-68 24-5p, hsa-miR-4648, hsa-miR-1227-5p, hsa-m iR-564, hsa-miR-3679-3p, hsa-miR-2861, hsa- miR-6737-5p, hsa-miR-4725-3p, hsa-miR-6716 -5p, hsa-miR-4675, hsa-miR-1915-3p, hsa-miR -671-5p, hsa-miR-3656, hsa-miR-6722-3p, hsa -miR-4707-5p, hsa-miR-4449, hsa-miR-1202, h sa-miR-4649-5p, hsa-miR-744-5p, hsa-miR-64 2a-3p, hsa-miR-451a, hsa-miR-6870-5p, hsa-m iR-4443, hsa-miR-6808-5p, hsa-miR-4728-5p, hsa-miR-937-5p, hsa-miR-135a-3p, hsa-miR-6 63b, hsa-miR-1343-5p, hsa-miR-6822-5p, hsa- miR-6803-5p, hsa-miR-6805-3p, hsa-miR-128- 2-5p, hsa-miR-4640-5p, hsa-miR-1469, hsa-mi R-92a-2-5p, hsa-miR-3940-5p, hsa-miR-4281, hsa-miR-1260b, hsa-miR-4758-5p, hsa-miR-19 15-5p, hsa-miR-5001-5p, hsa-miR-4286, hsa-m iR-6126, hsa-miR-6789-5p, hsa-miR-4459, hsa -miR-1268a, hsa-miR-6752-5p, hsa-miR-6131, hsa-miR-6800-5p, hsa-miR-4532, hsa-miR-687 2-3p, hsa-miR-718, hsa-miR-6769a-5p, hsa-mi R-4707-3p, hsa-miR-6765-5p, hsa-miR-4739, h sa-miR-4525, hsa-miR-4270, hsa-miR-4534, hs a-miR-6785-5p, hsa-miR-6850-5p, hsa-miR-46 97-5p, hsa-miR-1260a, hsa-miR-4486, hsa-miR -6880-5p, hsa-miR-6802-5p, hsa-miR-6861-5p 、hsa-miR-92b-5p, hsa-miR-1238-5p, hsa-miR- 6851-5p, hsa-miR-7704, hsa-miR-149-3p, hsa- miR-4689, hsa-miR-4688, hsa-miR-125a-3p, hs a-miR-23b-3p, hsa-miR-614, hsa-miR-1913, hs a-miR-16-5p, hsa-miR-6717-5p, hsa-miR-3648 、hsa-miR-3162-5p, hsa-miR-1909-3p, hsa-miR -8073, hsa-miR-6769b-5p, hsa-miR-6836-3p, h sa-miR-4484, hsa-miR-6819-5p, and hsa-miR-67 At least one miRNA selected from the group consisting of 94-5p can be used . Furthermore, other esophageal cancer markers that can be combined with these miRNAs, such as miRNAs consisting of hsa-miR-575 and hsa-miR-24-3p can also be preferably used as target nucleic acids. Furthermore, other esophageal cancer markers that can be combined with these miRNAs , namely, hsa-miR-675-5p, hsa-m iR-486-3p, hsa-miR-6777-5p, hsa-miR-4497, h sa-miR-296-3p, hsa-miR-6738-5p, hsa-miR-47 31-5p, hsa-miR-6889-5p, hsa-miR-6786-5p, hs a-miR-92a-3p, hsa-miR-4294, hsa-miR-4763-3 p, hsa-miR-6076, hsa-miR-663a, hsa-miR-760, hsa-miR-4667-5p, hsa-miR-6090, hsa-miR-473 0, hsa-miR-7106-5p, hsa-miR-3196, hsa-miR-5 698, hsa-miR-6087, hsa-miR-4665-5p, hsa-miR -8059 and at least one miRNA selected from the group consisting of hsa-miR-6879-5p can also be preferably used as target nucleic acids .

[0295] The above miRNAs include, for example, human genes containing the base sequences represented by any of SEQ ID NOs: 1 to 214 and 666 to 676 (that is, hsa-miR-204-3 respectively) p, hsa-miR-1247-3p, hsa-miR-6875-5p, hsa-mi R-6857-5p, hsa-miR-6726-5p, hsa-miR-3188, h sa-miR-8069, hsa-miR-4257, hsa-miR-1343-3p 、hsa-miR-7108-5p, hsa-miR-6825-5p, hsa-miR -7641, hsa-miR-3185, hsa-miR-4746-3p, hsa-m iR-6791-5p, hsa-miR-6893-5p, hsa-miR-4433b -3p, hsa-miR-3135b, hsa-miR-6781-5p, hsa-mi R-1908-5p, hsa-miR-4792, hsa-miR-7845-5p, h sa-miR-4417, hsa-miR-3184-5p, hsa-miR-1225 -5p, hsa-miR-1231, hsa-miR-1225-3p, hsa-miR -150-3p, hsa-miR-4433-3p, hsa-miR-6125, hsa -miR-4513, hsa-miR-6787-5p, hsa-miR-6784-5 p, hsa-miR-615-5p, hsa-miR-6765-3p, hsa-miR -5572, hsa-miR-6842-5p, hsa-miR-8063, hsa-m iR-6780b-5p, hsa-miR-187-5p, hsa-miR-128-1 -5p, hsa-miR-6729-5p, hsa-miR-6741-5p, hsa- miR-6757-5p, hsa-miR-7110-5p, hsa-miR-7975 、hsa-miR-1233-5p, hsa-miR-6845-5p, hsa-miR -3937, hsa-miR-4467, hsa-miR-7109-5p, hsa-m iR-6088, hsa-miR-6782-5p, hsa-miR-5195-3p, hsa-miR-4454, hsa-miR-6724-5p, hsa-miR-807 2, hsa-miR-4516, hsa-miR-6756-5p, hsa-miR-4 665-3p, hsa-miR-6826-5p, hsa-miR-6820-5p, h sa-miR-6887-5p, hsa-miR-3679-5p, hsa-miR-7 847-3p, hsa-miR-6721-5p, hsa-miR-3622a-5p, hsa-miR-939-5p, hsa-miR-602, hsa-miR-7977, hsa-miR-6749-5p, hsa-miR-1914-3p, hsa-miR- 4651, hsa-miR-4695-5p, hsa-miR-6848-5p, hsa -miR-1228-3p, hsa-miR-642b-3p, hsa-miR-674 6-5p, hsa-miR-3620-5p, hsa-miR-3131, hsa-mi R-6732-5p, hsa-miR-7113-3p, hsa-miR-23a-3p 、hsa-miR-3154, hsa-miR-4723-5p, hsa-miR-36 63-3p, hsa-miR-4734, hsa-miR-6816-5p, hsa-m iR-4442, hsa-miR-4476, hsa-miR-423-5p, hsa- miR-1249, hsa-miR-6515-3p, hsa-miR-887-3p, hsa-miR-4741, hsa-miR-6766-3p, hsa-miR-467 3, hsa-miR-6779-5p, hsa-miR-4706, hsa-miR-1 268b, hsa-miR-4632-5p, hsa-miR-3197, hsa-mi R-6798-5p, hsa-miR-711, hsa-miR-6840-3p, hs a-miR-6763-5p, hsa-miR-6727-5p, hsa-miR-37 1a-5p, hsa-miR-6824-5p, hsa-miR-4648, hsa-m iR-1227-5p, hsa-miR-564, hsa-miR-3679-3p, h sa-miR-2861, hsa-miR-6737-5p, hsa-miR-4725 -3p, hsa-miR-6716-5p, hsa-miR-4675, hsa-miR -1915-3p, hsa-miR-671-5p, hsa-miR-3656, hsa -miR-6722-3p, hsa-miR-4707-5p, hsa-miR-444 9, hsa-miR-1202, hsa-miR-4649-5p, hsa-miR-7 44-5p, hsa-miR-642a-3p, hsa-miR-451a, hsa-m iR-6870-5p, hsa-miR-4443, hsa-miR-6808-5p, hsa-miR-4728-5p, hsa-miR-937-5p, hsa-miR-1 35a-3p, hsa-miR-663b, hsa-miR-1343-5p, hsa- miR-6822-5p, hsa-miR-6803-5p, hsa-miR-6805 -3p, hsa-miR-128-2-5p, hsa-miR-4640-5p, hsa -miR-1469, hsa-miR-92a-2-5p, hsa-miR-3940- 5p, hsa-miR-4281, hsa-miR-1260b, hsa-miR-47 58-5p, hsa-miR-1915-5p, hsa-miR-5001-5p, hs a-miR-4286, hsa-miR-6126, hsa-miR-6789-5p, hsa-miR-4459, hsa-miR-1268a, hsa-miR-6752- 5p, hsa-miR-6131, hsa-miR-6800-5p, hsa-miR- 4532, hsa-miR-6872-3p, hsa-miR-718, hsa-miR -6769a-5p, hsa-miR-4707-3p, hsa-miR-6765-5 p, hsa-miR-4739, hsa-miR-4525, hsa-miR-4270 、hsa-miR-4534, hsa-miR-6785-5p, hsa-miR-68 50-5p, hsa-miR-4697-5p, hsa-miR-1260a, hsa- miR-4486, hsa-miR-6880-5p, hsa-miR-6802-5p 、hsa-miR-6861-5p, hsa-miR-92b-5p, hsa-miR- 1238-5p, hsa-miR-6851-5p, hsa-miR-7704, hsa -miR-149-3p, hsa-miR-4689, hsa-miR-4688, hs a-miR-125a-3p, hsa-miR-23b-3p, hsa-miR-614 、hsa-miR-1913, hsa-miR-16-5p, hsa-miR-6717 -5p, hsa-miR-3648, hsa-miR-3162-5p, hsa-miR -1909-3p, hsa-miR-8073, hsa-miR-6769b-5p, h sa-miR-6836-3p, hsa-miR-4484, hsa-miR-6819 -5p, hsa-miR-6794-5phsa-miR-575, hsa-miR-2 4-3p, hsa-miR-675-5p, hsa-miR-486-3p, hsa-m iR-6777-5p, hsa-miR-4497, hsa-miR-296-3p, h sa-miR-6738-5p, hsa-miR-4731-5p, hsa-miR-6 889-5p, hsa-miR-6786-5p, hsa-miR-92a-3p, hs a-miR-4294, hsa-miR-4763-3p, hsa-miR-6076, hsa-miR-663a, hsa-miR-760, hsa-miR-4667-5p , hsa-miR-6090, hsa-miR-4730, hsa-miR-7106- 5p, hsa-miR-3196, hsa-miR-5698, hsa-miR-608 7, hsa-miR-4665-5p, hsa-miR-8059 and hsa-miR- 6879-5p), its homologs, its transcription products, and its variants or derivatives are included. Here, the gene, homolog, transcription product, variant, and derivative are as defined above.

[0296] A preferred target nucleic acid is a human gene containing a nucleotide sequence represented by any of SEQ ID NOs: 1 to 700, or its transcription product, more preferably the transcription product, that is, miRNA, its precursor RNA, pri-miRNA or pre-miRNA.

[0297] The first target gene is the hsa-miR-204-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0298] The second target gene is the hsa-miR-1247-3p gene, its homologs, its transcripts, or their variants or derivatives. So far, there has been no report indicating that the change in the expression of this gene or its

[0299] transcripts can be a marker for esophageal cancer. The third target gene is the hsa-miR-6875-5p gene, its homologs, its transcripts, or their variants or derivatives. So far, there has been no report

[0300] indicating that the change in the expression of this gene or its transcripts can be a marker for esophageal cancer. The fourth target gene is the hsa-miR-6857-5p gene, its homologs, its

[0301] transcripts, or their variants or derivatives. So far, there has been no report indicating that the change in the expression of this gene or its transcripts can be a marker for esophageal cancer.

[0302] The fifth target gene is the hsa-miR-6726-5p gene, its homologs, its transcripts, or their variants or derivatives. So far, there has been no report indicating that the change in the expression of this gene or its

[0303] transcripts can be a marker for esophageal cancer. It is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer.

[0304] The eighth target gene is the hsa-miR-4257 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. It is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer.

[0305] The ninth target gene is the hsa-miR-1343-3p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. It is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer.

[0306] The tenth target gene is the hsa-miR-7108-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. It is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer.

[0307] The eleventh target gene is the hsa-miR-6825-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. It is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer.

[0308] The twelfth target gene is the hsa-miR-7641 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. It is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer. So far, there has been no report that changes in the expression of this gene or its transcription product can be used as a marker for esophageal cancer.

[0309] The 13th target gene is the hsa-miR-3185 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0310] The 14th target gene is the hsa-miR-4746-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0311] The 15th target gene is the hsa-miR-6791-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0312] The 16th target gene is the hsa-miR-6893-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0313] The 17th target gene is the hsa-miR-4433b-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0314] The 18th target gene is the hsa-miR-3135b gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0315] The 19th target gene is the hsa-miR-6781-5p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0316] The 20th target gene is the hsa-miR-1908-5p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0317] The 21st target gene is the hsa-miR-4792 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0318] The 22nd target gene is the hsa-miR-7845-5p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0319] The 23rd target gene is the hsa-miR-4417 gene, its homologs, their They are transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0320] The 24th target gene is the hsa-miR-3184-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0321] The 25th target gene is the hsa-miR-1225-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0322] The 26th target gene is the hsa-miR-1231 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0323] The 27th target gene is the hsa-miR-1225-3p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0324] The 28th target gene is the hsa-miR-150-3p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its There is no report that changes in the expression of the transcription product can be a marker for esophageal cancer.

[0325] The 29th target gene is the hsa-miR-4433-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0326] The 30th target gene is the hsa-miR-6125 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0327] The 31st target gene is the hsa-miR-4513 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0328] The 32nd target gene is the hsa-miR-6787-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0329] The 33rd target gene is the hsa-miR-6784-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0330] The 34th target gene is the hsa-miR-615-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report indicating that changes in the expression of this gene or its

[0331] transcription products can serve as markers for esophageal cancer. The 35th target gene is the hsa-miR-6765-3p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no

[0332] report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer. The 36th target gene is the hsa-miR-5572 gene, its homologs, their

[0333] transcription products, or their mutants or derivatives. So far, there has been no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0334] The 37th target gene is the hsa-miR-6842-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report indicating that changes in the expression of this gene or its

[0335] transcription products can serve as markers for esophageal cancer. They are those transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer. .

[0336] The 40th target gene is the hsa-miR-187-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0337] The 41st target gene is the hsa-miR-128-1-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer. .

[0338] The 42nd target gene is the hsa-miR-6729-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0339] The 43rd target gene is the hsa-miR-6741-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0340] The 44th target gene is the hsa-miR-6757-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its There is no report indicating that changes in the expression of the transcription products can serve as markers for esophageal cancer.

[0341] The 45th target gene is the hsa-miR-7110-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0342] The 46th target gene is the hsa-miR-7975 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0343] The 47th target gene is the hsa-miR-1233-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0344] The 48th target gene is the hsa-miR-6845-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0345] The 49th target gene is the hsa-miR-3937 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0346] The 50th target gene is the hsa-miR-4467 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0347] The 51st target gene is the hsa-miR-7109-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0348] The 52nd target gene is the hsa-miR-6088 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0349] The 53rd target gene is the hsa-miR-6782-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0350] The 54th target gene is the hsa-miR-5195-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0351] The 55th target gene is the hsa-miR-4454 gene, its homologs, their They are transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0352] The 56th target gene is the hsa-miR-6724-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0353] The 57th target gene is the hsa-miR-8072 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0354] The 58th target gene is the hsa-miR-4516 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0355] The 59th target gene is the hsa-miR-6756-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0356] The 60th target gene is the hsa-miR-4665-3p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its There is no report that changes in the expression of the transcription product can be a marker for esophageal cancer.

[0357] The 61st target gene is the hsa-miR-6826-5p gene, its homologs, its transcription products, or their variants or derivatives. There has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0358] The 62nd target gene is the hsa-miR-6820-5p gene, its homologs, its transcription products, or their variants or derivatives. There has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0359] The 63rd target gene is the hsa-miR-6887-5p gene, its homologs, its transcription products, or their variants or derivatives. There has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0360] The 64th target gene is the hsa-miR-3679-5p gene, its homologs, its transcription products, or their variants or derivatives. There has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0361] The 65th target gene is the hsa-miR-7847-3p gene, its homologs, its transcription products, or their variants or derivatives. There has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0362] The 66th target gene is the hsa-miR-6721-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0363] The 67th target gene is the hsa-miR-3622a-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer. .

[0364] The 68th target gene is the hsa-miR-939-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0365] The 69th target gene is the hsa-miR-602 gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0366] The 70th target gene is the hsa-miR-7977 gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0367] The 71st target gene is the hsa-miR-6749-5p gene, its homologs, They are these transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0368] The 72nd target gene is the hsa-miR-1914-3p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0369] The 73rd target gene is the hsa-miR-4651 gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0370] The 74th target gene is the hsa-miR-4695-5p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0371] The 75th target gene is the hsa-miR-6848-5p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0372] The 76th target gene is the hsa-miR-1228-3p gene, its homologs, its transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its There is no report indicating that changes in the expression of the transcription products can serve as markers for esophageal cancer.

[0373] The 77th target gene is the hsa-miR-642b-3p gene, its homologs, their transcription products, or their variants or derivatives. There is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0374] The 78th target gene is the hsa-miR-6746-5p gene, its homologs, their transcription products, or their variants or derivatives. There is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0375] The 79th target gene is the hsa-miR-3620-5p gene, its homologs, their transcription products, or their variants or derivatives. There is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0376] The 80th target gene is the hsa-miR-3131 gene, its homologs, their transcription products, or their variants or derivatives. There is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0377] The 81st target gene is the hsa-miR-6732-5p gene, its homologs, their transcription products, or their variants or derivatives. There is no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0378] The 82nd target gene is the hsa-miR-7113-3p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcripts can be a marker for esophageal cancer.

[0379] The 83rd target gene is the hsa-miR-23a-3p gene, its homologs, its transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcripts can be a marker for esophageal cancer.

[0380] The 84th target gene is the hsa-miR-3154 gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcripts can be a marker for esophageal cancer.

[0381] The 85th target gene is the hsa-miR-4723-5p gene, its homologs, its transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcripts can be a marker for esophageal cancer.

[0382] The 86th target gene is the hsa-miR-3663-3p gene, its homologs, its transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of this gene or its transcripts can be a marker for esophageal cancer.

[0383] The 87th target gene is the hsa-miR-4734 gene, its homologs, their They are transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0384] The 88th target gene is the hsa-miR-6816-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0385] The 89th target gene is the hsa-miR-4442 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of the gene or its transcription products can be markers for esophageal cancer.

[0386] The 90th target gene is the hsa-miR-4476 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0387] The 91st target gene is the hsa-miR-423-5p gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0388] The 92nd target gene is the hsa-miR-1249 gene, its homologs, its transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription products can be markers for esophageal cancer.

[0389] The 93rd target gene is the hsa-miR-6515-3p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0390] The 94th target gene is the hsa-miR-887-3p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0391] The 95th target gene is the hsa-miR-4741 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0392] The 96th target gene is the hsa-miR-6766-3p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0393] The 97th target gene is the hsa-miR-4673 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0394] The 98th target gene is the hsa-miR-6779-5p gene, its homologs, They are these transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0395] The 99th target gene is the hsa-miR-4706 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0396] The 100th target gene is the hsa-miR-1268b gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0397] The 101st target gene is the hsa-miR-4632-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0398] The 102nd target gene is the hsa-miR-3197 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its tran scription product can be a marker for esophageal cancer.

[0399] The 103rd target gene is the hsa-miR-6798-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or There is no report that the change in the expression of the transcription product can be a marker for esophageal cancer 。

[0400] The 104th target gene is the hsa-miR-711 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0401] The 105th target gene is the hsa-miR-6840-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that this gene or the change in the expression of its transcription product can be a marker for esophageal cancer 。

[0402] The 106th target gene is the hsa-miR-6763-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that this gene or the change in the expression of its transcription product can be a marker for esophageal cancer 。

[0403] The 107th target gene is the hsa-miR-6727-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that this gene or the change in the expression of its transcription product can be a marker for esophageal cancer 。

[0404] The 108th target gene is the hsa-miR-371a-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that this gene or There is no report that the change in the expression of the transcription product can be a marker for esophageal cancer 。

[0405] The 109th target gene is the hsa-miR-6824-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0406] The 110th target gene is the hsa-miR-4648 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0407] The 111th target gene is the hsa-miR-1227-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0408] The 112th target gene is the hsa-miR-564 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0409] The 113th target gene is the hsa-miR-3679-3p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0410] The 114th target gene is the hsa-miR-2861 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0411] The 115th target gene is the hsa-miR-6737-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0412] The 116th target gene is the hsa-miR-575 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is a report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer (Patent Document 1).

[0413] The 117th target gene is the hsa-miR-4725-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0414] The 118th target gene is the hsa-miR-6716-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0415] The 119th target gene is the hsa-miR-4675 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0416] The 120th target gene is the hsa-miR-1915-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0417] The 121st target gene is the hsa-miR-671-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0418] The 122nd target gene is the hsa-miR-3656 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0419] The 123rd target gene is the hsa-miR-6722-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0420] The 124th target gene is the hsa-miR-4707-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0421] The 125th target gene is the hsa-miR-4449 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0422] The 126th target gene is the hsa-miR-1202 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0423] The 127th target gene is the hsa-miR-4649-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0424] The 128th target gene is the hsa-miR-744-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0425] The 129th target gene is the hsa-miR-642a-3p gene, its homologs, They are those transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0426] The 130th target gene is the hsa-miR-451a gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0427] The 131st target gene is the hsa-miR-6870-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0428] The 132nd target gene is the hsa-miR-4443 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0429] The 133rd target gene is the hsa-miR-6808-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0430] The 134th target gene is the hsa-miR-4728-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or There is no report that changes in the expression of the transcription product can be a marker for esophageal cancer .

[0431] The 135th target gene is the hsa-miR-937-5p gene, its homologs, their transcription products, or their variants or derivatives. There is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0432] The 136th target gene is the hsa-miR-135a-3p gene, its homologs, their transcription products, or their variants or derivatives. There is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0433] The 137th target gene is the hsa-miR-663b gene, its homologs, their transcription products, or their variants or derivatives. There is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0434] The 138th target gene is the hsa-miR-1343-5p gene, its homologs, their transcription products, or their variants or derivatives. There is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0435] The 139th target gene is the hsa-miR-6822-5p gene, its homologs, their transcription products, or their variants or derivatives. There is no report that changes in the expression of this gene or There is no report indicating that the change in the expression of the transcription product can be a marker for esophageal cancer 。

[0436] The 140th target gene is the hsa-miR-6803-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0437] The 141st target gene is the hsa-miR-6805-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0438] The 142nd target gene is the hsa-miR-128-2-5p gene, its homologs 、their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0439] The 143rd target gene is the hsa-miR-4640-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0440] The 144th target gene is the hsa-miR-1469 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription There is no report indicating that the change in the expression of the writing product can be a marker for esophageal cancer.

[0441] The 145th target gene is the hsa-miR-92a-2-5p gene, its homologs , their transcripts, or their mutants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0442] The 146th target gene is the hsa-miR-3940-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0443] The 147th target gene is the hsa-miR-4281 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0444] The 148th target gene is the hsa-miR-1260b gene, its homologs, their transcripts, or their mutants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0445] The 149th target gene is the hsa-miR-4758-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcript can be a marker for esophageal cancer. ​​​

[0446] The 150th target gene is the hsa-miR-1915-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0447] The 151st target gene is the hsa-miR-5001-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0448] The 152nd target gene is the hsa-miR-4286 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0449] The 153rd target gene is the hsa-miR-6126 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0450] The 154th target gene is the hsa-miR-6789-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0451] The 155th target gene is the hsa-miR-4459 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0452] The 156th target gene is the hsa-miR-1268a gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0453] The 157th target gene is the hsa-miR-6752-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0454] The 158th target gene is the hsa-miR-6131 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0455] The 159th target gene is the hsa-miR-6800-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0456] The 160th target gene is the hsa-miR-4532 gene, its homologs, is a transcription product, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0457] The 161st target gene is the hsa-miR-6872-3p gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0458] The 162nd target gene is the hsa-miR-718 gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0459] The 163rd target gene is the hsa-miR-6769a-5p gene, its homologs , their transcription products, or variants or derivatives thereof. So far, there has been no report that this gene or changes in the expression of its transcription product can be a marker for esophageal cancer. None.

[0460] The 164th target gene is the hsa-miR-4707-3p gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0461] The 165th target gene is the hsa-miR-6765-5p gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that this gene or There is no report indicating that the change in the expression of its transcription product can be a marker for esophageal cancer 。

[0462] The 166th target gene is the hsa-miR-4739 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0463] The 167th target gene is the hsa-miR-4525 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0464] The 168th target gene is the hsa-miR-4270 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0465] The 169th target gene is the hsa-miR-4534 gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0466] The 170th target gene is the hsa-miR-6785-5p gene, its homologs, their transcription products, or their variants or derivatives. So far, there is no report indicating that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0467] The 171st target gene is the hsa-miR-6850-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0468] The 172nd target gene is the hsa-miR-4697-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0469] The 173rd target gene is the hsa-miR-1260a gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0470] The 174th target gene is the hsa-miR-4486 gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0471] The 175th target gene is the hsa-miR-6880-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0472] The 176th target gene is the hsa-miR-6802-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0473] The 177th target gene is the hsa-miR-6861-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0474] The 178th target gene is the hsa-miR-92b-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0475] The 179th target gene is the hsa-miR-1238-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0476] The 180th target gene is the hsa-miR-6851-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0477] The 181st target gene is the hsa-miR-7704 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report indicating that changes in the expression of this gene or its

[0478] transcription products can serve as markers for esophageal cancer. The 182nd target gene is the hsa-miR-149-3p gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no

[0479] report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer. The 183rd target gene is the hsa-miR-4689 gene, its homologs,

[0480] their transcription products, or their variants or derivatives. So far, there has been no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0481] The 184th target gene is the hsa-miR-4688 gene, its homologs, their transcription products, or their variants or derivatives. So far, there has been no report indicating that changes in the expression of this gene or its transcription products can serve as markers for esophageal cancer.

[0482] The 185th target gene is the hsa-miR-125a-3p gene, its homologs, They are these transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0483] The 187th target gene is the hsa-miR-614 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0484] The 188th target gene is the hsa-miR-1913 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its trans cription product can be a marker for esophageal cancer.

[0485] The 189th target gene is the hsa-miR-16-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0486] The 190th target gene is the hsa-miR-675-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0487] The 191st target gene is the hsa-miR-486-3p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its There is no report that changes in the expression of the transcript can be a marker for esophageal cancer.

[0488] The 192nd target gene is the hsa-miR-6777-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcript can be a marker for esophageal cancer. 。

[0489] The 193rd target gene is the hsa-miR-4497 gene, its homologs, their transcripts, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0490] The 194th target gene is the hsa-miR-296-3p gene, its homologs, their transcripts, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0491] The 195th target gene is the hsa-miR-6738-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcript can be a marker for esophageal cancer. 。

[0492] The 196th target gene is the hsa-miR-4731-5p gene, its homologs, their transcripts, or their variants or derivatives. So far, there is no report that changes in the expression of this gene or its transcript can be a marker for esophageal cancer. 。

[0493] The 197th target gene is the hsa-miR-6889-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0494] The 198th target gene is the hsa-miR-6786-5p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0495] The 199th target gene is the hsa-miR-92a-3p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been a report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer (Patent Document 1).

[0496] The 200th target gene is the hsa-miR-4294 gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer.

[0497] The 201st target gene is the hsa-miR-4763-3p gene, its homologs, their transcripts, or their mutants or derivatives. So far, there has been no report that the change in the expression of this gene or its transcript can be a marker for esophageal cancer .

[0498] The 202nd target gene is the hsa-miR-6076 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0499] The 203rd target gene is the hsa-miR-663a gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is a report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer (Patent Document 1).

[0500] The 204th target gene is the hsa-miR-760 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0501] The 205th target gene is the hsa-miR-4667-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer .

[0502] The 206th target gene is the hsa-miR-6090 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that the change in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0503] The 207th target gene is the hsa-miR-4730 gene, its homologs, their is a transcription product thereof, or a variant or derivative thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0504] The 208th target gene is the hsa-miR-7106-5p gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer. .

[0505] The 209th target gene is the hsa-miR-3196 gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0506] The 210th target gene is the hsa-miR-5698 gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0507] The 211th target gene is the hsa-miR-6087 gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer.

[0508] The 212th target gene is the hsa-miR-4665-5p gene, its homologs, their transcription products, or variants or derivatives thereof. So far, there has been no report that changes in the expression of this gene or There is no report that changes in the expression of the transcription product can be a marker for esophageal cancer 。

[0509] The 213th target gene is the hsa-miR-8059 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer

[0510] The 214th target gene is the hsa-miR-6879-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that changes in the expression of this gene or its transcription product can be a marker for esophageal cancer 。

[0511] The 215th target gene is the hsa-miR-6717-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that changes in the expression of the gene or its transcription product can be a marker for esophageal cancer

[0512] The 216th target gene is the hsa-miR-3648 gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that changes in the expression of the gene or its transcription product can be a marker for esophageal cancer

[0513] The 217th target gene is the hsa-miR-3162-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there is no report that changes in the expression of the gene or its transcription product can be a marker for esophageal cancer

[0514] The 218th target gene is the hsa-miR-1909-3p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of a gene or its transcript can be a marker for esophageal cancer.

[0515] The 219th target gene is the hsa-miR-8073 gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of a gene or its transcript can be a marker for esophageal cancer.

[0516] The 220th target gene is the hsa-miR-6769b-5p gene, its homologs their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of a gene or its transcript can be a marker for esophageal cancer. .

[0517] The 221st target gene is the hsa-miR-6836-3p gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of a gene or its transcript can be a marker for esophageal cancer.

[0518] The 222nd target gene is the hsa-miR-4484 gene, its homologs, their transcripts, or their variants or derivatives. So far, there has been no report that changes in the expression of a gene or its transcript can be a marker for esophageal cancer.

[0519] The 223rd target gene is the hsa-miR-6819-5p gene, its homologs, They are their transcription products, or their mutants or derivatives. So far, there has been no report that changes in the expression of genes or their transcription products can be markers for esophageal cancer. So far, there has been no report that changes in the expression of genes or their transcription products can be markers for esophageal cancer.

[0520] The 224th target gene is the hsa-miR-6794-5p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been no report that changes in the expression of genes or their transcription products can be markers for esophageal cancer. So far, there has been no report that changes in the expression of genes or their transcription products can be markers for esophageal cancer.

[0521] The 225th target gene is the hsa-miR-24-3p gene, its homologs, their transcription products, or their mutants or derivatives. So far, there has been a report that changes in the expression of genes or their transcription products can be markers for esophageal cancer (Patent Document 1). So far, there has been a report that changes in the expression of genes or their transcription products can be markers for esophageal cancer (Patent Document 1). (Patent Document 1). 2. Nucleic Acid Probe or Primer for Detecting Esophageal Cancer In the present invention, a nucleic acid capable of specifically binding to the target nucleic acid as the above esophageal cancer marker can be used as a nucleic acid for detecting or diagnosing esophageal cancer, for example, a nucleic acid probe or primer. In the present invention, a nucleic acid probe or primer that can be used for detecting or diagnosing esophageal cancer can be the target nucleic acid as the above esophageal cancer marker, for example, hsa-miR-204-3p, hsa-miR-1247-3p, hsa

[0522] In the present invention, a nucleic acid probe or primer that can be used for detecting esophageal cancer or for diagnosing esophageal cancer is the target nucleic acid as the above esophageal cancer marker, for example, hsa-miR-204-3p, hsa-miR-1247-3p, hsa -miR-6875-5p, hsa-miR-6857-5p, hsa-miR-672 6-5p, hsa-miR-3188, hsa-miR-8069, hsa-miR-4 257, hsa-miR-1343-3p, hsa-miR-7108-5p, hsa- miR-6825-5p, hsa-miR-7641, hsa-miR-3185, hs a-miR-4746-3p, hsa-miR-6791-5p, hsa-miR-68 93-5p, hsa-miR-4433b-3p, hsa-miR-3135b, hsa -miR-6781-5p, hsa-miR-1908-5p, hsa-miR-479 2, hsa-miR-7845-5p, hsa-miR-4417, hsa-miR-3 184-5p, hsa-miR-1225-5p, hsa-miR-1231, hsa- miR-1225-3p, hsa-miR-150-3p, hsa-miR-4433- 3p, hsa-miR-6125, hsa-miR-4513, hsa-miR-678 7-5p, hsa-miR-6784-5p, hsa-miR-615-5p, hsa- miR-6765-3p, hsa-miR-5572, hsa-miR-6842-5p , hsa-miR-8063, hsa-miR-6780b-5p, hsa-miR-1 87-5p, hsa-miR-128-1-5p, hsa-miR-6729-5p, h sa-miR-6741-5p, hsa-miR-6757-5p, hsa-miR-7 110-5p, hsa-miR-7975, hsa-miR-1233-5p, hsa- miR-6845-5p, hsa-miR-3937, hsa-miR-4467, hs a-miR-7109-5p, hsa-miR-6088, hsa-miR-6782- 5p, hsa-miR-5195-3p, hsa-miR-4454, hsa-miR- 6724-5p, hsa-miR-8072, hsa-miR-4516, hsa-mi R-6756-5p, hsa-miR-4665-3p, hsa-miR-6826-5 p, hsa-miR-6820-5p, hsa-miR-6887-5p, hsa-mi R-3679-5p, hsa-miR-7847-3p, hsa-miR-6721-5 p, hsa-miR-3622a-5p, hsa-miR-939-5p, hsa-mi R-602, hsa-miR-7977, hsa-miR-6749-5p, hsa-m iR-1914-3p, hsa-miR-4651, hsa-miR-4695-5p, hsa-miR-6848-5p, hsa-miR-1228-3p, hsa-miR- 642b-3p, hsa-miR-6746-5p, hsa-miR-3620-5p, hsa-miR-3131, hsa-miR-6732-5p, hsa-miR-711 3-3p, hsa-miR-23a-3p, hsa-miR-3154, hsa-miR -4723-5p, hsa-miR-3663-3p, hsa-miR-4734, hs a-miR-6816-5p, hsa-miR-4442, hsa-miR-4476, hsa-miR-423-5p, hsa-miR-1249, hsa-miR-6515 -3p, hsa-miR-887-3p, hsa-miR-4741, hsa-miR- 6766-3p, hsa-miR-4673, hsa-miR-6779-5p, hsa -miR-4706, hsa-miR-1268b, hsa-miR-4632-5p, hsa-miR-3197, hsa-miR-6798-5p, hsa-miR-711 、hsa-miR-6840-3p, hsa-miR-6763-5p, hsa-miR -6727-5p, hsa-miR-371a-5p, hsa-miR-6824-5p , hsa-miR-4648, hsa-miR-122...

Claims

1. A kit for detecting esophageal cancer, comprising a nucleic acid probe capable of specifically binding to a polynucleotide of miR-615-5p consisting of the nucleotide sequence represented by SEQ ID NO: 34 and / or a primer capable of specifically recognizing and amplifying the polynucleotide.

2. The kit is another esophageal cancer marker, consisting of the base sequences represented by SEQ ID NOs: 2-3, 5-7, 9-33, 35-214, and 666-676, namely miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6784-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734,miR-6816-5p, miR-4442, miR-4476, miR-423-5p, miR-1249, miR-6515-3p, miR-887-3p, miR-4741, miR-6766-3p, miR-4673, miR-6779-5p, miR-4706, miR-1268b, miR-4632-5p, miR-3197, miR-6798-5p, miR-711, miR-6840-3p, miR-6763-5p, miR-6727-5p, miR-371a-5p, miR-6824-5p, miR-4648, miR-1227-5p, miR-564, miR-3679-3p, miR-2861, miR-6737-5p, miR-4725-3p, miR-6716-5p, miR-4675, miR-1915-3p, miR-671-5p, miR-3656, miR-6722-3p, miR-4707-5p, miR-4449, miR-1202, miR-4649-5p, miR-744-5p, miR-642a-3p, miR-451a, miR-6870-5p, miR-4443, miR-6808-5p, miR-4728-5p, miR-937-5p, miR-135a-3p, miR-663b, miR-1343-5p, miR-6822-5p, miR-6803-5p, miR-6805-3p, miR-128-2-5p, miR-4640-5p, miR-1469, miR-92a-2-5p, miR-3940-5p, miR-4281, miR-1260b, miR-4758-5p, miR-1915-5p, miR-5001-5p, miR-4286, miR-6126, miR-6789-5p, miR-4459, miR-1268a, miR-6752-5p, miR-6131, miR-6800-5p, miR-4532, miR-6872-3p, miR-718, miR-6769a-5p, miR-4707-3p, miR-6765-5p, miR-4739, miR-4525, miR-4270, miR-4534, miR-6785-5p, miR-6850-5p, miR-4697-5p, miR-1260a, miR-4486, miR-6880-5p, miR-6802-5p, miR-6861-5p, miR-92b-5p, miR-1238-5p, miR-6851-5pAt least one polynucleotide selected from the group consisting of miR-7704, miR-149-3p, miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p, miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p, miR-6836-3p, miR-4484, miR-6819-5p, and miR-6794-5p, miR-575, miR-24-3p, miR-675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, and a nucleic acid probe capable of specifically binding to the polynucleotide and / or a primer capable of specifically recognizing and amplifying the polynucleotide. The kit according to claim 1.

3. A device for detecting esophageal cancer, comprising a nucleic acid probe capable of specifically binding to a polynucleotide of miR-615-5p consisting of the nucleotide sequence represented by SEQ ID NO: 34 and / or a primer capable of specifically recognizing and amplifying the polynucleotide.

4. The device is another esophageal cancer marker, and consists of base sequences represented by SEQ ID NOs: 2 to 3, 5 to 7, 9 to 33, 35 to 214, and 666 to 676, namely miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6784-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734,miR-6816-5p, miR-4442, miR-4476, miR-423-5p, miR-1249, miR-6515-3p, miR-887-3p, miR-4741, miR-6766-3p, miR-4673, miR-6779-5p, miR-4706, miR-1268b, miR-4632-5p, miR-3197, miR-6798-5p, miR-711, miR-6840-3p, miR-6763-5p, miR-6727-5p, miR-371a-5p, miR-6824-5p, miR-4648, miR-1227-5p, miR-564, miR-3679-3p, miR-2861, miR-6737-5p, miR-4725-3p, miR-6716-5p, miR-4675, miR-1915-3p, miR-671-5p, miR-3656, miR-6722-3p, miR-4707-5p, miR-4449, miR-1202, miR-4649-5p, miR-744-5p, miR-642a-3p, miR-451a, miR-6870-5p, miR-4443, miR-6808-5p, miR-4728-5p, miR-937-5p, miR-135a-3p, miR-663b, miR-1343-5p, miR-6822-5p, miR-6803-5p, miR-6805-3p, miR-128-2-5p, miR-4640-5p, miR-1469, miR-92a-2-5p, miR-3940-5p, miR-4281, miR-1260b, miR-4758-5p, miR-1915-5p, miR-5001-5p, miR-4286, miR-6126, miR-6789-5p, miR-4459, miR-1268a, miR-6752-5p, miR-6131, miR-6800-5p, miR-4532, miR-6872-3p, miR-718, miR-6769a-5p, miR-4707-3p, miR-6765-5p, miR-4739, miR-4525, miR-4270, miR-4534, miR-6785-5p, miR-6850-5p, miR-4697-5p, miR-1260a, miR-4486, miR-6880-5p, miR-6802-5p, miR-6861-5p, miR-92b-5p, miR-1238-5p, miR-6851-5pAnucleic acid probe that can specifically bind to at least one polynucleotide selected from the group consisting of miR-7704, miR-149-3p, miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p, miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p, miR-6836-3p, miR-4484, miR-6819-5p, miR-6794-5p, miR-575, miR-24-3p, miR-675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, and / or a primer that specifically recognizes and amplifies the polynucleotide. The device according to claim 3.

5. The device according to claim 3 or 4, wherein the device is a device for measurement by a hybridization technique.

6. The device according to claim 5, wherein the hybridization technique is a nucleic acid array technique.

7. Using the kit according to claim 1 or the device according to claim 3 or claims 5 or 6 dependent on claim 3, measuring the expression level of miR-615-5p in a specimen of a subject, comparing the measured expression level of the subject with the control expression level of a healthy subject measured in the same manner, and when the expression level of the subject is lower than the expression level of the healthy subject, evaluating in vitro that the subject is suffering from esophageal cancer, a method for assisting in the detection of esophageal cancer.

8. The method according to claim 7, wherein the subject is a human.

9. The method according to claim 7 or 8, wherein the specimen is blood, serum or plasma.

10. Use of miR-615-5p consisting of the nucleotide sequence represented by SEQ ID NO: 34 as an esophageal cancer marker for detecting esophageal cancer, measuring the expression level of the miR-615-5p in a specimen of a subject, comparing the measured expression level with the control expression level of a healthy subject measured in the same manner, and when the expression level of the subject is lower than the control expression level, evaluating that the subject is suffering from esophageal cancer said use.

11. The miR-615-5p consists of base sequences represented by SEQ ID NOs: 2 to 3, 5 to 7, 9 to 33, 35 to 214, and 666 to 676, and is miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6784-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5p,miR-4442, miR-4476, miR-423-5p, miR-1249, miR-6515-3p, miR-887-3p, miR-4741, miR-6766-3p, miR-4673, miR-6779-5p, miR-4706, miR-1268b, miR-4632-5p, miR-3197, miR-6798-5p, miR-711, miR-6840-3p, miR-6763-5p, miR-6727-5p, miR-371a-5p, miR-6824-5p, miR-4648, miR-1227-5p, miR-564, miR-3679-3p, miR-2861, miR-6737-5p, miR-4725-3p, miR-6716-5p, miR-4675, miR-1915-3p, miR-671-5p, miR-3656, miR-6722-3p, miR-4707-5p, miR-4449, miR-1202, miR-4649-5p, miR-744-5p, miR-642a-3p, miR-451a, miR-6870-5p, miR-4443, miR-6808-5p, miR-4728-5p, miR-937-5p, miR-135a-3p, miR-663b, miR-1343-5p, miR-6822-5p, miR-6803-5p, miR-6805-3p, miR-128-2-5p, miR-4640-5p, miR-1469, miR-92a-2-5p, miR-3940-5p, miR-4281, miR-1260b, miR-4758-5p, miR-1915-5p, miR-5001-5p, miR-4286, miR-6126, miR-6789-5p, miR-4459, miR-1268a, miR-6752-5p, miR-6131, miR-6800-5p, miR-4532, miR-6872-3p, miR-718, miR-6769a-5p, miR-4707-3p, miR-6765-5p, miR-4739, miR-4525, miR-4270, miR-4534, miR-6785-5p, miR-6850-5p, miR-4697-5p, miR-1260a, miR-4486, miR-6880-5p, miR-6802-5p, miR-6861-5p, miR-92b-5p, miR-1238-5p, miR-6851-5p, miR-7704The use according to claim 10, which is used as an esophageal cancer marker for the detection of esophageal cancer, in combination with at least one polynucleotide selected from the group consisting of miR-149-3p, miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p, miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p, miR-6836-3p, miR-4484, miR-6819-5p, miR-6794-5p, miR-575, miR-24-3p, miR-675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059 and miR-6879-5p.,

Images