Biochemical instruments

Abstract

The present invention aims to provide a biochemical instrument capable of effectively suppressing adsorption of biochemical substances on a surface that comes into contact with a biochemical sample. The biochemical instrument of the present invention is characterized in that the portion that comes into contact with a biochemical sample is made of a resin composition containing at least one cyclic olefin polymer selected from the group consisting of a copolymer of a cyclic olefin and a linear olefin, a ring-opening polymer of a cyclic olefin, and a hydrogenated product of a ring-opening polymer of a cyclic olefin, and an antioxidant, the resin composition containing 0.01 to 0.7 parts by mass of the antioxidant per 100 parts by mass of the cyclic olefin polymer.

Description

Technical Field

[0001] The present invention relates to a biochemical instrument that comes into contact with a biochemical sample.

Background Art

[0002] Cyclic olefin resins are used in various applications because they are excellent in melt processability, fluidity, heat shrinkability, printing characteristics, etc. In addition to the above characteristics, since they are also excellent in transparency, chemical resistance, moisture resistance, mechanical properties, etc., the use of such resins has been extended to applications such as biochemical instruments.

[0003] Here, in applications such as biochemical instruments, molded articles made of resin compositions are used for storage containers, measuring instruments, etc. that handle substances derived from living organisms (biochemical substances) such as proteins and nucleic acids. However, when handling samples containing relatively low-concentration biochemical substances, problems may occur due to the adsorption of biochemical substances on the surface of the molded article. Specifically, during operations such as storage, transportation, measurement, dilution, and analysis, the adsorption of biochemical substances in the sample on the surface of the biochemical instrument causes various problems such as measurement errors, decreased sensitivity, reduction of the content, and disappearance of trace samples.

[0004] Therefore, in biochemical instruments using materials containing cyclic olefin resins, development of methods for suppressing the adsorption of biochemical substances at the contact surface with the sample has been carried out. Specifically, for example, in Patent Document 1, a surface treatment method of a molded article is used, which includes a step of subjecting the surface of a molded article made of a material containing a cyclic olefin resin to plasma discharge treatment and a step of bringing the surface of the molded article into contact with a strong acid. By treating the surface of the molded article in this way, it has been proposed to suppress the adsorption of biochemical substances on the surface of the molded article. In addition, in Patent Document 2, the surface of a molded article made of a material containing a cyclic olefin resin is irradiated with vacuum ultraviolet light, and the surface of the molded article is treated using a surface treatment method of forming a self-assembled monolayer on the irradiated surface. By doing so, it has been proposed to suppress the adsorption of biochemical substances on the surface of the molded article.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0006] However, there is still room for improvement in the biochemical instrument made of the above-mentioned conventional molded body in terms of suppressing the adsorption of biochemical substances on the contact surface with a sample containing a biochemical substance (biochemical substance sample). Therefore, an object of the present invention is to provide a biochemical instrument capable of favorably suppressing the adsorption of biochemical substances on the contact surface with a biochemical substance sample.

Means for Solving the Problems

[0007] The present inventor earnestly studied for the purpose of solving the above problems. Then, the present inventor found that a biochemical instrument in which the portion in contact with the biochemical substance sample is made of a predetermined resin composition can favorably suppress the adsorption of biochemical substances on the contact surface with the biochemical substance sample, and completed the present invention.

[0008] That is, the object of the present invention is to advantageously solve the above problems. The biochemical instrument of the present invention is a biochemical instrument with which a biochemical substance sample comes into contact, and the portion that comes into contact with the biochemical substance sample is composed of a resin composition containing at least one cyclic olefin polymer selected from the group consisting of a copolymer of a cyclic olefin and a linear olefin, a ring-opening polymer of a cyclic olefin, and a hydrogenated product of a ring-opening polymer of a cyclic olefin, and an antioxidant. The resin composition is characterized by containing 0.01 parts by mass or more and 0.7 parts by mass or less of the antioxidant with respect to 100 parts by mass of the cyclic olefin polymer. Thus, if the portion that comes into contact with the biochemical substance sample in the biochemical instrument is formed of a resin composition containing a predetermined cyclic olefin polymer and a predetermined amount of antioxidant, adsorption of the biochemical substance can be favorably suppressed. In the present invention, the content of the antioxidant in the resin composition can be measured by the method described in the examples of this specification.

[0009] Here, in the biochemical instrument of the present invention, it is preferable that the antioxidant contains a hindered phenol-based antioxidant. If the antioxidant contains a hindered phenol-based antioxidant, adsorption of the biochemical substance on the contact surface with the biochemical substance sample can be more favorably suppressed.

[0010] Further, in the biochemical instrument of the present invention, it is preferable that the contact angle of the portion that comes into contact with the biochemical substance sample with respect to water is 85° or more. If the contact angle of the portion that comes into contact with the biochemical substance sample with respect to water is the above value or more, adsorption of the biochemical substance on the contact surface with the biochemical substance sample can be more favorably suppressed. In the present invention, the contact angle of the portion that comes into contact with the biochemical substance sample with respect to water can be measured by the method described in the examples of this specification.

Effects of the Invention

[0011] According to the present invention, it is possible to provide a biochemical instrument that can favorably suppress the adsorption of a biochemical substance on the contact surface with the biochemical substance sample.

Brief Description of the Drawings

[0012]

Figure 1

Embodiments for Carrying Out the Invention

[0013] Hereinafter, embodiments of the present invention will be described in detail.

[0014] (Biochemical instrument) The biochemical instrument of the present invention is a biochemical instrument that comes into contact with a biochemical substance sample, has a portion that comes into contact with the biochemical substance sample, and optionally has a portion that does not come into contact with the biochemical substance sample. And, the biochemical instrument of the present invention is characterized in that the portion that comes into contact with the biochemical substance sample is made of a resin composition containing a predetermined cyclic olefin polymer and a predetermined amount of an antioxidant. The biochemical instrument of the present invention can favorably suppress the adsorption of biochemical substances on the contact surface with the biochemical substance sample. Specific examples of the biochemical instrument of the present invention include, for example, instruments as described on pages 13 to 30 of "Biological Experiments from the Basics" (published by Sankyo) (May 2002), and more specifically, beakers, flasks, petri dishes, pipettes, syringes, centrifuge tubes, needles, tubes, Eppendorf tips, titer plates, microchannels, filters, test cells, storage containers, containers for analytical instruments, etc. can be mentioned. However, the biochemical instrument of the present invention is not limited to the above, and includes any instrument that may come into contact with a sample containing a biochemical substance.

[0015] <Biochemical substance sample> The biochemical substance sample that comes into contact with the biochemical instrument of the present invention is not particularly limited as long as it is a sample containing a biochemical substance, but usually refers to a liquid sample containing a biochemical substance dissolved or dispersed in an arbitrary solvent. And, the biochemical substance sample may contain any other components other than the biochemical substance. Here, examples of biochemical substances include proteins, enzymes, antibodies, polypeptides, oligopeptides, amino acids, nucleic acids, lipids, polysaccharides, oligosaccharides, amino sugars, microorganisms, viruses, etc. Note that nucleic acids can include both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). Also, biochemical substances are not limited to those obtained by methods such as extraction from biological materials, and also include those chemically synthesized outside the body. And as biochemical substances, nucleic acids are particularly preferred. If the biochemical substance contains nucleic acids, adsorption of the biochemical substance at the contact surface with the biochemical substance sample in the biochemical instrument can be further better suppressed. The solvent is not particularly limited as long as it can dissolve or disperse the biochemical substance. For example, water and the like are used. The concentration of the biochemical substance in the biochemical substance sample is not particularly limited, but is preferably 10000 mg / L or less, more preferably 1000 mg / L or less, and even more preferably 100 mg / L or less. If the concentration of the biochemical substance in the biochemical substance sample is 10000 mg / L or less, adsorption of the biochemical substance at the contact surface with the biochemical substance sample in the biochemical instrument can be further better suppressed.

[0016] <Contact part> The part in the biochemical instrument of the present invention that comes into contact with the biochemical substance sample (hereinafter, may be simply referred to as the "contact part") is made of a resin composition containing a predetermined cyclic olefin polymer and a predetermined amount of antioxidant. Here, the contact part is not particularly limited as long as it is a part in the biochemical instrument that can come into contact with the biochemical substance sample, and can have any shape, area, and volume. Specific examples of the contact part include the inner walls of containers such as beakers, flasks, storage containers, etc., and the inner and outer walls of measuring instruments such as pipettes and Eppendorf tips.

[0017] The physical properties of the contact portion are not particularly limited, but the contact angle of the contact portion with respect to water is preferably 85° or more, more preferably 87° or more. If the contact angle of the contact portion with respect to water is 85° or more, adsorption of biochemical substances at the contact surface with the biochemical substance sample in the biochemical instrument can be further suppressed more favorably. Further, the contact angle of the contact portion with respect to water is preferably 100° or less, more preferably 95° or less.

[0018] In addition, the contact portion may be subjected to various surface treatments for the purpose of adjusting the contact angle with respect to water described above. The surface treatment is not particularly limited, and includes physicochemical treatments such as plasma discharge treatment, corona discharge treatment, flame treatment, ultraviolet treatment, electron beam treatment, radiation treatment; chemical treatments such as chemical agent treatment, vapor treatment, surface grafting treatment; mechanical treatments such as sandblast treatment, embossing treatment, and the like.

[0019] [Resin Composition] The resin composition constituting the contact portion contains a predetermined cyclic olefin polymer and a predetermined amount of antioxidant, and may optionally further contain other components.

[0020] - Cyclic Olefin Polymer - The cyclic olefin polymer includes at least one cyclic olefin polymer selected from the group consisting of a copolymer of a cyclic olefin and a chain olefin, a ring-opening polymer of a cyclic olefin, and a hydrogenated product of a ring-opening polymer of a cyclic olefin. And from the viewpoint of enhancing the strength of the resin composition and the contact portion made of the resin composition, it is preferable to use a hydrogenated product of a ring-opening polymer of a cyclic olefin as the cyclic olefin polymer.

[0021] - Copolymer of Cyclic Olefin and Chain Olefin The copolymer of a cyclic olefin and a chain olefin is usually a polymer in which a cyclic olefin and a chain olefin are addition-copolymerized.

[0022] Specific examples of cyclic olefins include monocyclic cyclic olefins such as cyclopentene, cyclohexene, cyclooctene, cyclopentadiene, 1,3-cyclohexadiene;

[0023] Bicyclo[2.2.1]hepta-2-ene (common name: norbornene, hereinafter sometimes abbreviated as "NB"), 5-methyl-bicyclo[2.2.1]hepta-2-ene, 5,5-dimethyl-bicyclo[2.2.1]hepta-2-ene, 5-ethyl-bicyclo[2.2.1]hepta-2-ene, 5-butyl-bicyclo[2.2.1]hepta-2-ene, 5-ethylidene-bicyclo[2.2.1]hepta-2-ene, 5-hexyl-bicyclo[2.2.1]hepta-2-ene, 5-octyl-bicyclo[2.2.1]hepta-2-ene, 5-octadecyl-bicyclo[2.2.1]hepta-2-ene, 5-methylidene-bicyclo[2.2.1]hepta-2-ene, 5-vinyl-bicyclo[2.2.1]hepta-2-ene, 5-propenyl-bicyclo[2.2.1]hepta-2-ene and other bicyclic cyclic olefins;

[0024] Tricyclo[5.2.1.0 2,6 deca-3,8-diene (common name: dicyclopentadiene, hereinafter sometimes abbreviated as "DCP"), tricyclo[5.2.1.0 2,6 deca-3-ene, tricyclo[6.2.1.0 2,7 undeca-3,9-diene, tricyclo[6.2.1.0 2,7 undeca-4,9-diene, tricyclo[6.2.1.0 2,7 undeca-9-ene, 5-cyclopentyl-bicyclo[2.2.1]hepta-2-ene, 5-cyclohexyl-bicyclo[2.2.1]hepta-2-ene, 5-cyclohexenylbicyclo[2.2.1]hepta-2-ene, 5-phenyl-bicyclo[2.2.1]hepta-2-ene and other tricyclic cyclic olefins;

[0025] Tetracyclo[6.2.1.1 3,6 .0 2,7Dodeca-4-ene (also simply referred to as "tetracyclododecene". Hereinafter, it may be abbreviated as "TCD".), 9-methyltetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, 9-ethyltetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene (hereinafter, it may be abbreviated as "ETD"), 9-methylidenetetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, 9-ethylidenetetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, 9-vinyltetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, 9-propenyl-tetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, tetracyclo[9.2.1.0 2,10 .0 3,8 tetradeca-3,5,7,12-tetraene (also referred to as 1,4-methano-1,4,4a,9a-tetrahydrofluorene. Hereinafter, it may be abbreviated as "MTF"), tetracyclo[10.2.1.0 2,11 .0 4,9 pentadeca-4,6,8,13-tetraene (also referred to as 1,4-methano-1,4,4a,9,9a,10-hexahydroanthracene), etc., four-ring cyclic olefins;

[0026] 9-cyclopentyl-tetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, 9-cyclohexyl-tetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, 9-cyclohexenyl-tetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, pentacyclo[6.6.1.1 3,6 .0 2,7 .0 9,14 -4-hexadecene, pentacyclo[6.5.1.1 3,6 .0 2,7 .09,13 -4-pentadecene, pentacyclo[7.4.0.0 2,7 .1 3,6 .1 10,13 -4-pentadecene, 9-phenyl-cyclopentyl-tetracyclo[6.2.1.1 3,6 .0 2,7 dodeca-4-ene, heptacyclo[8.7.0.1 2,9 .1 4,7 .1 11,17 .0 3,8 .0 12,16 -5-eicosene, heptacyclo[8.7.0.1 2,9 .0 3,8 .1 4,7 .0 12,17 .1 13,16 -14-eicosene and other cyclic olefins with 5 or more rings can be mentioned. These cyclic olefins can be used alone or in combination of two or more.

[0027] Specific examples of the chain olefin are not particularly limited as long as it is copolymerizable with the above-mentioned cyclic olefin. For example, linear or branched olefins having 2 to 20 carbon atoms such as ethylene, propylene, butene, pentene, hexene, butadiene, pentadiene, and hexadiene can be mentioned.

[0028] The method for preparing the copolymer of the cyclic olefin and the chain olefin is not particularly limited, and a known method for copolymerizing the above-mentioned cyclic olefin and chain olefin can be used.

[0029] - Ring-opening polymer of cyclic olefin The ring-opening polymer of the cyclic olefin is a polymer obtained by ring-opening polymerization of one or more kinds of cyclic olefins. As the cyclic olefin, the same ones as those used for preparing the copolymer of the cyclic olefin and the chain olefin described above can be used. The method for preparing the ring-opening polymer of cyclic olefin is not particularly limited, and known methods for ring-opening polymerization of the above-described cyclic olefins, such as metathesis polymerization, can be used.

[0030] - Hydrogenated product of the ring-opening polymer of cyclic olefin The hydrogenated product of the ring-opening polymer of cyclic olefin is obtained by hydrogenating the above-described ring-opening polymer of cyclic olefin. The method for hydrogenating the ring-opening polymer of cyclic olefin is not particularly limited, and known methods can be used. For example, a known hydrogenation catalyst containing a transition metal such as nickel and palladium can be added to a solution of the above ring-opening polymer of cyclic olefin to hydrogenate the carbon-carbon double bond in the ring-opening polymer. Also, the hydrogenation rate is preferably 90% or more, more preferably 95% or more, still more preferably 99% or more, and even more preferably 99.6% or more. The hydrogenation rate can be measured by the method described in the examples of this specification.

[0031] - Physical properties of the cyclic olefin-based polymer The physical properties of the above-described cyclic olefin-based polymer are not particularly limited. For example, the glass transition temperature of the cyclic olefin-based polymer is preferably 60°C or higher, more preferably 100°C or higher, and still more preferably 130°C or higher. If the glass transition temperature of the cyclic olefin-based polymer is 60°C or higher, the adsorption of biochemical substances on the contact surface with the biochemical substance sample in the biochemical instrument can be further suppressed better. In the present invention, the glass transition temperature of the cyclic olefin-based polymer can be measured in accordance with JIS K 6911.

[0032] - Content of the cyclic olefin-based polymer The content of the cyclic olefin polymer in the resin composition is preferably 70% by mass or more, more preferably 80% by mass or more, and still more preferably 90% by mass or more. If the content of the cyclic olefin polymer is 70% by mass or more, adsorption of biochemical substances at the contact surface with biochemical substance samples in biochemical instruments can be suppressed more favorably.

[0033] - Antioxidant - As the antioxidant, for example, primary antioxidants such as hindered phenol-based antioxidants and amine-based antioxidants, and secondary antioxidants such as phosphorus-based antioxidants and sulfur-based antioxidants can be used.

[0034] Specific examples of the hindered phenol-based antioxidants include pentaerythritol-tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate], 3,9-bis{2-[3-(3-t-butyl-4-hydroxy-5-methylphenyl)propionyloxy]-1,1-dimethylethyl}2,4,8,10-tetraoxaspiro[5,5]undecane, octadecyl-3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate, 1,6-hexanediol-bis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate], 1,3,5-trimethyl-2,4,6-tris(3,5-di-t-butyl-4-hydroxybenzyl)benzene, 2,6-di-t-butyl-4-methylphenol, 2,6-di-t-butyl-4-ethylphenol, 2,6-diphenyl-4-octadecyloxyphenol, stearyl(3,5-di-t-butyl-4-hydroxyphenyl)propionate, thiodiethylene glycol bis[(3,5-di-t-butyl-4-hydroxyphenyl)propionate], 4,4’-thiobis(6-t-butyl-m-cresol), 2,2’-methylenebis(4-methyl-6-t-butylphenol), 2,2’-methylenebis(4-ethyl-6-t-butylphenol), bis[3,3-bis(4-hydroxy-3-t-butylphenyl)butyric acid] glycol ester, 4,4’-butylidenebis(6-t-butyl-m-cresol), 2,2’-ethylidenebis(4,6-di-t-butylphenol), 2,2’-ethylidenebis(4-s-butyl-6-t-butylphenol), 1,1,3-tris(2-methyl-4-hydroxy-5-t-butylphenyl)butane, bis[2-t-butyl-4-methyl-6-(2-hydroxy-3-t-butyl-5-methylbenzyl)phenyl] terephthalate, 1,3,5-tris(2,6-dimethyl-3-hydroxy-4-t-butylbenzyl)isocyanurate, 1,3,5-tris(3,5-di-t-butyl-4-hydroxybenzyl)-2,4,6-trimethylbenzene, 1,3,5-tris[(3,5-di-t-butyl-4-hydroxyphenyl)propionyloxyethyl]isocyanurate, tetrakis[methylene-3-(3,Alkyl-substituted hindered phenolic antioxidants such as [[ID=]],

[0035] Specific examples of amine antioxidants include hindered amine compounds such as 2,2,6,6-tetramethyl-4-piperidyl stearate, 1,2,2,6,6-pentamethyl-4-piperidyl stearate, 1-hydroxy-2,2,6,6-tetramethylpiperidinol, 2,2,6,6-tetramethyl-4-piperidyl benzoate, bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate, bis(1,2,2,6,6-pentamethyl-4-piperidyl) sebacate, bis(2,2,6,6-tetramethyl-4-piperidyl)·di(tridecyl)-1,2,3,4-butanetetracarboxylate, bis(1,2,2,6,6-pentamethyl-4-piperidyl)·di(tridecyl)-1,2,3,4-butanetetracarboxylate, 1-(2-hydroxyethyl)-2,2,6,6-tetramethyl-4-piperidinol / succinic acid diethyl polycondensate, 1,6-bis(2,2,6,6-tetramethyl-4-piperidylamino)hexane / dibromoethane polycondensate, 1,6-bis(2,2,6,6-tetramethyl-4-piperidylamino)hexane / 2,4-dichloro-6-morpholino-s-triazine polycondensate, 1,6-bis(2,2,6,6-tetramethyl-4-piperidylamino)hexane / 2,4-dichloro-6-tert-octylamino-s-triazine polycondensate, 1,5,8,12-tetrakis[2,4-bis(N-butyl-N-(2,2,6,6-tetramethyl-4-piperidyl)amino)-s-triazin-6-yl]-1,5,8,12-tetraazadodecane, 1,5,8,12-tetrakis[2,4-bis(N-butyl-N-(1,2,2,6,6-pentamethyl-4-piperidyl)amino)-s-triazin-6-yl]-1,5,8,12-tetraazadodecane; and dialkylhydroxylamine compounds such as diethylhydroxylamine, dioctylhydroxylamine, didodecylhydroxylamine, dioctadecylhydroxylamine, etc.

[0036] Specific examples of phosphorus-based antioxidants include bis-(2,6-di-t-butyl-4-methylphenyl)pentaerythritol diphosphite, tris(2,4-di-t-butylphenyl phosphite), tetrakis(2,4-di-t-butyl-5-methylphenyl)-4,4'-biphenylene diphosphonite, bis-(2,6-dicumylphenyl)pentaerythritol diphosphite, 2,2-methylenebis(4,6-di-t-butylphenyl) octyl phosphite, bis(2,4-di-t-butylphenyl)pentaerythritol-di-phosphite, bis(2,6-di-t-butyl-4-methoxycarbonylethyl-phenyl)pentaerythritol diphosphite, bis(2,6-di-t-butyl-4-octadecyloxycarbonylethyl-phenyl)pentaerythritol diphosphite, and the like.

[0037] Specific examples of sulfur-based antioxidants include dilauryl 3,3'-thiodipropionate, dimyristyl 3,3'-thiodipropionate, distearyl 3,3'-thiodipropionate, lauryl stearyl 3,3'-thiodipropionate, pentaerythritol-tetrakis-(β-lauryl-thio-propionate), 3,9-bis(2-dodecylthioethyl)-2,4,8,10-tetraoxaspiro[5,5]undecane, and the like.

[0038] From the viewpoint of favorably suppressing the adsorption of biochemical substances at the contact portion, it is preferable to use a hindered phenol-based antioxidant as the antioxidant. Among them, it is more preferable to use pentaerythritol tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate].

[0039] The above-mentioned antioxidants can be used alone or in combination of two or more.

[0040] The content of the antioxidant in the resin composition needs to be 0.01 part by mass or more, preferably 0.05 part by mass or more, more preferably 0.09 part by mass or more, and needs to be 0.7 part by mass or less, preferably 0.6 part by mass or less, more preferably 0.5 part by mass or less, based on 100 parts by mass of the above-mentioned cyclic olefin polymer. If the content of the antioxidant is 0.01 part by mass or more and 0.7 part by mass or less based on 100 parts by mass of the cyclic olefin polymer, adsorption of the biochemical substance on the contact surface with the biochemical substance sample in the biochemical instrument can be suppressed well.

[0041] - Other components - In addition to the above-mentioned cyclic olefin polymer and antioxidant, the resin composition may contain any components within the range where the desired effect can be obtained. Specifically, examples of the optional components include additives that can be used in the preparation of cyclic olefin polymers such as chain transfer agents, polymerization regulators, polymerization reaction retardants, and reactive fluidizing agents; polymers other than cyclic olefin polymers such as rubbery polymers and thermoplastic elastomers; organic or inorganic fillers; inorganic fine particles; resin additives such as flame retardants, ultraviolet absorbers, weather stabilizers, antistatic agents, slip agents, metal soaps, antifogging agents, plasticizers; oils such as natural oils and synthetic oils; mold release agents; fluorescent brighteners; dyes; pigments; colorants; antibacterial agents; deodorants; odor removers, etc. Note that as the above-mentioned optional components, generally used compounds such as those described in JP-A-2009-242568, those described in JP-A-2010-100683, and those described in Patent No. 5613981 can be used.

[0042] - Method for preparing resin composition - The method for preparing the resin composition is not particularly limited, and known methods can be used. For example, it can be prepared by melt-kneading the above-mentioned predetermined cyclic olefin polymer, a predetermined amount of antioxidant, and any other components using a single-screw extruder or a twin-screw extruder. Note that the temperature during melt-kneading is not particularly limited, but is preferably 180°C or higher, more preferably 200°C or higher, still more preferably 220°C or higher, preferably 350°C or lower, more preferably 320°C or lower, and still more preferably 300°C or lower.

[0043] <Part not in contact with the biochemical sample> The biochemical instrument of the present invention optionally has a part not in contact with the biochemical substance. Specific examples of the part not in contact with the biochemical substance include the outer wall of containers such as beakers and flasks, and the gripping part of measuring instruments such as pipettes. Note that in the biochemical instrument of the present invention, the part not in contact with the biochemical sample may be formed of the above resin composition containing a predetermined cyclic olefin polymer and a predetermined amount of antioxidant, or may be formed of a resin composition other than the above resin composition, a material other than the above resin composition such as metal or ceramic.

[0044] <Method for manufacturing a biochemical instrument> The method for manufacturing a biochemical instrument is not particularly limited as long as the manufactured biochemical instrument has the above-described contact part. For example, a method of molding the above resin composition into the shape of a biochemical instrument is used. In addition, a method of coating the contact part of a biochemical instrument made of a material other than the above resin composition with the above resin composition can also be used. The method for molding the resin composition is not particularly limited, and known methods can be used. Specifically, methods such as injection molding, injection compression molding, gas assist injection molding, extrusion molding, multilayer extrusion molding, rotational molding, hot press molding, blow molding, foam molding, and molding by a 3D printer can be mentioned. The conditions during molding of the resin composition are not particularly limited. For example, in the case of injection molding, the temperature of the resin composition is preferably 180°C or higher, more preferably 200°C or higher, still more preferably 220°C or higher, preferably 350°C or lower, more preferably 320°C or lower, and still more preferably 300°C or lower. In addition, known methods for coating include dipping method; coating method using a brush or the like; spraying method; coating method using a coater such as a roll coater, a bar coater, or a knife coater.

Example

[0045] Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited thereto. In addition, parts and % in the present examples are based on mass unless otherwise specified. In production examples, examples, and comparative examples, the hydrogenation rate and glass transition temperature of the cyclic olefin-based polymer; the content of the antioxidant in the resin composition; the contact angle of the contact portion with respect to water; the DNA adsorption rate; and the linearity of the dilution series data were measured, calculated, or evaluated using the following methods.

[0046] <Hydrogenation rate> Regarding the ring-opening polymers of cyclic olefins obtained in Production Examples 1 to 3 and the hydrogenated products of the polymers, using deuterated chloroform as a solvent 1 1H-NMR measurement was performed, and the hydrogenation rate in the hydrogenation reaction was determined by calculating the ratio of the unsaturated bonds that disappeared among all the unsaturated bonds present in the ring-opening polymer of the cyclic olefin.

[0047] <Glass transition temperature> The glass transition temperature of the cyclic olefin-based polymer obtained in each production example was measured based on JIS K 6911 using a differential scanning calorimeter (manufactured by Nanotronics Co., Ltd., DSC6220S11).

[0048] <Content of antioxidant in resin composition> Eppendorf-type tube containers obtained in each of the examples and comparative examples (hereinafter, may be simply referred to as "containers") were used, and a 0.1-mm sheet was produced at 200 °C in a nitrogen atmosphere using a hot press machine. Using this sheet, an IR spectrum was measured by the FT-IR transmission method, the ratio between the peak of the antioxidant and the peak of the cyclic olefin polymer was determined, and the content (parts by mass) of the antioxidant with respect to 100 parts by mass of the cyclic olefin polymer in the resin composition was quantified using a calibration curve. As the measuring instrument for the IR spectrum, "AVATAR360" manufactured by Thermo Scientific was used.

[0049] <Contact angle of the contact part with respect to water> The contact angle with respect to water of the part where the biochemical sample in the containers obtained in each of the examples and comparative examples comes into contact is defined as the static contact angle measured by the curve fitting method using a contact angle meter Drop Master 300 manufactured by Kyowa Interface Science Co., Ltd.

[0050] <DNA adsorption rate> When the operation of transferring the DNA solution as the biochemical sample to the container was repeated, the ratio of DNA adsorbed to the container (DNA adsorption rate) was calculated by the following procedure. 1) As the biochemical sample, a DNA standard sample ("Deoxyribonucleic acid aqueous solution for quantitative analysis NMIJ CRM 6205-a", DNA strand length 600 bps) was used to prepare a three-step dilution series of DNA concentrations of 1000 mg / L, 100 mg / L, and 10 mg / L. 2) Among the above dilution series, a DNA solution with an arbitrary concentration was dispensed into the container to be evaluated. 3) The container was tapped about 20 times to apply the DNA solution to the inner wall surface of the container, thereby bringing the DNA into contact with the inner wall surface of the container. 4) The DNA solution remaining on the inner wall surface of the container was spun down using a tabletop centrifuge. 5) An amount of the DNA solution in the container for analysis was left, and the rest was transferred to an unused container to be evaluated. The DNA solution left for analysis was used as the sample after one transfer operation. 6) For the DNA solution transferred to an unused container, the operations in 3) to 5) above were repeated 9 times to obtain samples after 2 to 10 transfer operations. 7) Transfer the sample before the transfer operation of the DNA solution used in 2) above and the samples after 1 to 10 transfer operations obtained in the above operations to an analysis tube, and perform analysis by SEC-UV. Thus, the peak area (A0) derived from the amount of DNA in the sample before the transfer operation and the peak areas (A1 to A 10 ) derived from the remaining amount of DNA in the DNA solution after 1 to 10 container transfer operations were obtained. Then, the DNA adsorption rates (B1 to B 10 )(%) after 1 to 10 container transfer operations were calculated by the following formula. B n ={(A0 - A n ) / A0} × 100 (n is the number of container transfer operations) The smaller the value of the obtained DNA adsorption rate (B n ), the better the suppression of the adsorption of the biochemical substance at the contact surface with the biochemical substance sample in the container to be evaluated. Note that the measurement conditions of SEC-UV are as follows. HPLC: LC-10Avp system (Shimadzu Corporation) Column: Yarra-2000 (Phenomenex) Eluent: 0.1 mol / L Tris-HCl (pH 8.1) Detection: UV detector (260 nm)

[0051] <Linearity of dilution series data> As a biochemical substance sample, a DNA standard sample ("Aqueous solution of deoxyribonucleic acid for quantitative analysis NMIJ CRM 6205-a", DNA strand length 600 bps) was used to prepare a five-step dilution series with DNA concentrations of 10 μg / L, 50 μg / L, 100 μg / L, 500 μg / L, and 1000 μg / L in the container to be evaluated. For each dilution series, in order to bring the DNA into contact with the inner wall surface of the container, the container was tapped about 20 times to apply the DNA solution to the inner wall surface of the container, and then spun down using a tabletop centrifuge. Each dilution series was analyzed by SEC-UV, and a scatter plot of dilution series data with the peak area obtained on the vertical axis and the DNA concentration on the horizontal axis was created, and its linearity was evaluated. The higher the linearity of the obtained dilution series data, the less the adsorption of DNA to the container, indicating that in the container to be evaluated, the adsorption of the biochemical substance at the contact surface with the biochemical substance sample can be well suppressed. Note that the measurement conditions of SEC-UV are the same as those in the measurement of the above-mentioned DNA adsorption rate.

[0052] (Production Example 1) Production of Hydrogenated Product A of Ring-Opening Polymer of Cyclic Olefin Under a nitrogen atmosphere, 0.82 part of 1-hexene, 0.15 part of dibutyl ether, and 0.30 part of triisobutylaluminum were placed in a reactor at room temperature (25 °C) and mixed with 500 parts of dehydrated cyclohexane. Then, while maintaining the temperature at 45 °C, 76 parts of tricyclo[4.3.0.1 2,5 deca-3,7-diene (DCP), and 70 parts of tetracyclo[4.4.0.1 2,5 .1 7,10 dodeca-3-ene (TCD), and 54 parts of tetracyclo[7.4.0.0 2,7 .1 10,13 tetradeca-2,4,6,11-tetraene (MTF), and 80 parts of tungsten hexachloride (0.7% toluene solution) were continuously added and polymerized in parallel over 2 hours. Next, 1.06 parts of butyl glycidyl ether and 0.52 part of isopropyl alcohol were added to the polymerization solution to inactivate the polymerization catalyst and stop the polymerization reaction. When the reaction solution containing the obtained ring-opening polymer was analyzed by gas chromatography, the polymerization conversion rate of each monomer was 99.5%.

[0053] Next, 270 parts of cyclohexane was added to 100 parts of the reaction solution containing the obtained ring-opening polymer, and further 5 parts of a diatomaceous earth-supported nickel catalyst (G-96D manufactured by Nissan Girdler Co., Ltd.; nickel supported ratio 58%) as a hydrogenation catalyst was added. After pressurizing to 5 MPa with hydrogen and heating to 200 °C with stirring, the mixture was reacted for 8 hours to obtain a reaction solution containing a hydrogenated product of the DCP / TCD / MTF ring-opening copolymer. The hydrogenation catalyst was removed by filtration. Next, using a cylindrical concentration dryer (manufactured by Hitachi, Ltd.), cyclohexane as a solvent and other volatile components were removed from the solution at a temperature of 270 °C and a pressure of 1 kPa or less. Then, the hydrogenated product was extruded in a strand form from an extruder in a molten state and pelletized after cooling to obtain pellets. The hydrogenation ratio of this pelletized hydrogenated product of the ring-opening copolymer (hydrogenated product A of the ring-opening polymer of cyclic olefin) was 99.8%, and the glass transition temperature was 136 °C.

[0054] (Production Example 2) Production of hydrogenated product B of ring-opening polymer of cyclic olefin Into a reactor at room temperature (25 °C) under a nitrogen atmosphere, 250 parts of dehydrated cyclohexane was placed, and further 0.84 part of 1-hexene, 0.06 part of dibutyl ether, and 0.11 part of triisobutylaluminum were added and mixed. Then, while maintaining the temperature at 45 °C, 85 parts of DCP, 8-ethyltetracyclo 2,5 .1 7,10 dodeca-3-ene (ETD) 15 parts, and 15 parts of tungsten hexachloride (0.7% toluene solution) were continuously added over 2 hours and polymerized. When the reaction solution containing the obtained ring-opening polymer was analyzed by gas chromatography, the polymerization conversion rate of each monomer was 100%. The obtained polymerization reaction solution was transferred to a pressure-resistant hydrogenation reactor, 5 parts of a diatomaceous earth-supported nickel catalyst (G-96D manufactured by Nissan Girdler Co., Ltd.; nickel supported ratio 58%) as a hydrogenation catalyst and 100 parts of cyclohexane were added, and the mixture was reacted at 150 °C and a hydrogen pressure of 4.4 MPa for 8 hours. This reaction solution was pressure-filtered (Hundafilter, manufactured by Ishikawajima-Harima Heavy Industries Co., Ltd.) at a pressure of 0.25 MPa using diatomaceous earth (Radiolite #500 manufactured by Showa Chemical Industry Co., Ltd.) as a filter bed to remove the hydrogenation catalyst. Next, in the same manner as in Production Example 1, pellets of the hydrogenated ring-opening copolymer (hydrogenated product B of the ring-opening polymer of cyclic olefin) were obtained. The hydrogenation rate of this pelletized hydrogenated ring-opening copolymer was 99.6%, and the glass transition temperature was 102°C.

[0055] (Production Example 3) Production of hydrogenated product C of ring-opening polymer of cyclic olefin Into a dried and nitrogen-substituted polymerization reactor, 7 parts of a monomer mixture of bicyclo[2.2.1]hepta-2-ene (norbornene, NB), DCP, and TCD (mass ratio 38 / 31 / 31), 1600 parts of dehydrated cyclohexane, 3.5 parts of 1-hexene as a molecular weight regulator, 1.3 parts of diisopropyl ether, 0.33 part of isobutyl alcohol, 0.84 part of triisobutylaluminum, and 30 parts of a 0.66% cyclohexane solution of tungsten hexachloride were added, and the mixture was stirred at 55°C for 10 minutes. Next, while maintaining the reaction system at 55°C and with stirring, 93 parts of a monomer mixture having the same composition as the above-described monomer mixture and 72 parts of a 0.77% cyclohexane solution of tungsten hexachloride were each continuously dropped into the polymerization reactor over 150 minutes. After further stirring for 30 minutes after the completion of the dropping, 1.0 part of isopropyl alcohol was added to terminate the polymerization reaction. When the polymerization reaction solution was measured by gas chromatography, the conversion rate of the monomer to the polymer was 100%.

[0056] Next, 300 parts of the polymerization reaction solution containing the above polymer was transferred to an autoclave equipped with a stirrer, and 100 parts of cyclohexane and 2.0 parts of a nickel catalyst supported on diatomaceous earth (manufactured by JGC Catalysts and Chemicals Ltd., "T8400RL", nickel supported ratio 58%) were added. After replacing the inside of the autoclave with hydrogen, the reaction was carried out at 170°C under a hydrogen pressure of 4.9 MPa for 6 hours. This solution was filtered through a filter made of a stainless steel wire mesh equipped with diatomaceous earth (manufactured by Showa Chemical Industry Co., Ltd., "Radiolite #500") as a filter aid to remove the catalyst. The obtained reaction solution was poured into 8000 parts of isopropyl alcohol with stirring to precipitate the hydride, which was then collected by filtration. Further, after washing with 500 parts of acetone, 0.13×103 It was dried for 24 hours in a vacuum dryer set at 65 °C under a pressure of Pa or less to obtain a hydrogenated product of the ring-opening copolymer. Next, in the same manner as in Production Example 1, pellets of a hydrogenated product of a ring-opening copolymer (hydrogenated product C of a ring-opening polymer of a cyclic olefin) were obtained. The hydrogenation rate of this pelletized hydrogenated product of the ring-opening copolymer was 99.9%, and the glass transition temperature was 68 °C.

[0057] (Production Example 4) Production of copolymer D of cyclic olefin and linear olefin To a reaction vessel charged with 258 L of cyclohexane, NB (120 kg) was added at room temperature (25 °C) under a nitrogen stream, and stirring was carried out for 5 minutes. Further, triisobutylaluminum was added so that the concentration in the system became 1.0 mL / L. Next, while stirring, ethylene was passed through at normal pressure to make the inside of the system an ethylene atmosphere. The internal temperature of the autoclave was maintained at 70 °C, and the internal pressure was pressurized with ethylene to a gauge pressure of 6 kg / cm 2 After stirring for 10 minutes, a copolymerization reaction of ethylene and NB was initiated by adding 0.4 L of a toluene solution containing isopropylidene(cyclopentadienyl)(indenyl)zirconium dichloride and methylaluminoxane to the system. At this time, the catalyst concentration was 0.018 mmol / L of isopropylidene(cyclopentadienyl)(indenyl)zirconium dichloride and 8.0 mmol / L of methylaluminoxane with respect to the whole system.

[0058] During the polymerization, the temperature was maintained at 70 °C and the internal pressure was maintained at a gauge pressure of 6 kg / cm 2 by continuously supplying ethylene into the system. After 60 minutes, the polymerization reaction was stopped by adding isopropyl alcohol. After depressurization, the polymer solution was taken out, and then it was brought into contact with an aqueous solution obtained by adding 5 L of concentrated hydrochloric acid to 1 m 3 of water with strong stirring at a ratio of 1:1 to transfer the catalyst residue to the aqueous phase. After allowing this contact mixture to stand, the aqueous phase was separated and removed, and further washed twice with water to purify and separate the organic phase.

[0059] Next, the purified and separated polymerization solution was brought into contact with three times the amount of acetone under strong stirring to precipitate the copolymer. Then, the solid part (copolymer) was collected by filtration and thoroughly washed with acetone. Further, in order to extract the unreacted monomers present in the polymer, this solid part was put into acetone so that it became 40 kg / m 3 and then an extraction operation was carried out under the conditions of 60 °C for 2 hours. After the extraction treatment, the solid part was collected by filtration and dried at 130 °C and 350 mmHg for 12 hours under a nitrogen flow to obtain an ethylene-NB copolymer (copolymer D of a cyclic olefin and a linear olefin). Next, in the same manner as in Production Example 1, pellets of an ethylene-NB copolymer (copolymer D of a cyclic olefin and a linear olefin) were obtained. The glass transition temperature of this pelletized ethylene-NB copolymer was 138 °C.

[0060] (Example 1) 100 parts of the hydrogenated product A of the ring-opening polymer of the cyclic olefin obtained in Production Example 1 and 0.01 part of pentaerythritol tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate] as an antioxidant were mixed using a blender, and then kneaded and extruded at a cylinder temperature of 290 °C using a twin-screw kneader with the hopper purged with nitrogen to obtain a pelletized resin composition A-1. Next, using an injection molding machine ROBOSHOTα100B manufactured by FANUC, the pelletized resin composition A-1 was injection molded under the conditions of a resin composition temperature of 300 °C and a mold temperature of 100 °C to produce an Eppendorf-type tube container with a volume of 1.5 mL. Using this container, the content of the antioxidant in the resin composition, the contact angle of the inner wall surface (contact part) where the biochemical sample contacts in the Eppendorf-type tube container with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0061] (Example 2) A resin composition A-2 was obtained in the same manner as in Example 1 except that the addition amount of the antioxidant was 0.1 part. Then, a container was produced by injection molding in the same manner as in Example 1, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, the DNA adsorption rate, and the linearity of the dilution series data were measured, calculated, or evaluated. The results are shown in Table 1 and FIG. 1.

[0062] (Example 3) A resin composition A-3 was obtained in the same manner as in Example 1 except that the addition amount of the antioxidant was 0.5 part. Then, a container was produced by injection molding in the same manner as in Example 1, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0063] (Comparative Example 1) A resin composition A-4 was obtained in the same manner as in Example 1 except that no antioxidant was added. Then, a container was produced by injection molding in the same manner as in Example 1, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0064] (Comparative Example 2) A resin composition A-5 was obtained in the same manner as in Example 1 except that the addition amount of the antioxidant was 0.75 part. Then, a container was produced by injection molding in the same manner as in Example 1, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0065] (Example 4) 100 parts of the hydrogenated product B of the cyclic olefin obtained in Production Example 2 and 0.01 part of pentaerythritol tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate] as an antioxidant were mixed with a blender, and kneaded and extruded at a cylinder temperature of 285°C using a twin-screw kneader whose popper was purged with nitrogen to obtain a pelletized resin composition B-1. Next, using an injection molding machine ROBOSHOT α100B manufactured by FANUC, the pelletized resin composition B-1 was injection molded under the conditions of a resin composition temperature of 290°C and a mold temperature of 80°C to produce an Eppendorf-type tube container with a capacity of 1.5 mL. Using this container, the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0066] (Example 5) A resin composition B-2 was obtained in the same manner as in Example 4 except that the addition amount of the antioxidant was 0.1 part. Then, a container was produced by injection molding in the same manner as in Example 4, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, the DNA adsorption rate, and the linearity of the dilution series data were measured, calculated, or evaluated. The results are shown in Table 1 and Figure 1.

[0067] (Example 6) A resin composition B-3 was obtained in the same manner as in Example 4 except that the addition amount of the antioxidant was 0.5 part. Then, a container was produced by injection molding in the same manner as in Example 4, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0068] (Comparative Example 3) A resin composition B-4 was obtained in the same manner as in Example 4 except that no antioxidant was added. Then, a container was produced by injection molding in the same manner as in Example 4, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0069] (Comparative Example 4) A resin composition B-5 was obtained in the same manner as in Example 4 except that the addition amount of the antioxidant was 0.75 part. Then, a container was produced by injection molding in the same manner as in Example 4, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 1.

[0070] (Example 7) 100 parts by weight of the hydrogenated product of the ring-opening polymer of the cyclic olefin obtained in Production Example 3 and 0.01 part by weight of pentaerythritol tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate] as an antioxidant were mixed with a blender, and kneaded and extruded at a cylinder temperature of 260°C using a twin-screw kneader with the hopper purged with nitrogen to obtain a pelletized resin composition C-1. Next, using an injection molding machine ROBOSHOTα100B manufactured by FANUC, the pelletized resin composition C-1 was injection molded under the conditions of a resin composition temperature of 250°C and a mold temperature of 40°C to produce an Eppendorf-type tube container with a volume of 1.5 mL. Using this container, the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0071] (Example 8) A resin composition C-2 was obtained in the same manner as in Example 7 except that the addition amount of the antioxidant was 0.1 part by weight. Then, a container was produced by injection molding in the same manner as in Example 7, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0072] (Example 9) A resin composition C-3 was obtained in the same manner as in Example 7 except that the addition amount of the antioxidant was 0.5 part by weight. Then, a container was produced by injection molding in the same manner as in Example 7, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0073] (Comparative Example 5) A resin composition C-4 was obtained in the same manner as in Example 7 except that no antioxidant was added. Then, a container was produced by injection molding in the same manner as in Example 7, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0074] (Comparative Example 6) A resin composition C-5 was obtained in the same manner as in Example 7, except that the addition amount of the antioxidant was 0.75 parts. Then, in the same manner as in Example 7, a container was produced by injection molding, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0075] (Example 10) 100 parts of the copolymer D1 of cyclic olefin and linear olefin obtained in Production Example 4 and 0.01 part of pentaerythritol tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate] as an antioxidant were mixed with a blender and kneaded and extruded at a cylinder temperature of 290 °C using a twin-screw kneader with the hopper purged with nitrogen to obtain a pelletized resin composition D-1. Next, using an injection molding machine ROBOSHOTα100B manufactured by FANUC, the pelletized resin composition D-1 was injection molded under the conditions of a resin composition temperature of 300 °C and a mold temperature of 100 °C to produce a 1.5 mL-capacity Eppendorf-type tube container. Using this container, the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0076] (Example 11) A resin composition D-2 was obtained in the same manner as in Example 10, except that the addition amount of the antioxidant was 0.1 part. Then, in the same manner as in Example 10, a container was produced by injection molding, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0077] (Example 12) A resin composition D-3 was obtained in the same manner as in Example 10, except that the addition amount of the antioxidant was 0.5 part. Then, in the same manner as in Example 10, a container was produced by injection molding, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0078] (Comparative Example 7) Resin composition D-4 was obtained in the same manner as in Example 10 except that no antioxidant was added. Then, a container was produced by injection molding in the same manner as in Example 10, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0079] (Comparative Example 8) Resin composition D-5 was obtained in the same manner as in Example 10 except that the amount of the antioxidant added was 0.75 part. Then, a container was produced by injection molding in the same manner as in Example 10, and the content of the antioxidant in the resin composition, the contact angle of the contact portion with respect to water, and the DNA adsorption rate were measured or calculated. The results are shown in Table 2.

[0080] (Comparative Example 9) Using an injection molding machine ROBOSHOTα100B manufactured by FANUC, polypropylene (manufactured by Sumitomo Chemical Co., Ltd., "Exxelene (registered trademark) AR244M", melting point measured by ISO3146: 157 °C) was injection molded under the conditions of a resin composition temperature of 240 °C and a mold temperature of 70 °C to produce a 1.5 mL capacity Eppendorf-type tube container. Using this container, the contact angle of the contact portion with respect to water, the DNA adsorption rate, and the linearity of the dilution series data were measured, calculated, or evaluated. The results are shown in Table 2 and FIG. 1.

[0081] (Comparative Example 10) Using a commercially available 1.5 mL capacity Eppendorf-type tube container made of polypropylene (manufactured by Corning, "Axygen Maximum Recovery Tube") with surface treatment for improving smoothness, the contact angle of the contact portion with respect to water, the DNA adsorption rate, and the linearity of the dilution series data were calculated or evaluated. The results are shown in Table 2 and FIG. 1.

[0082]

Table 1

[0083]

Table 2

[0084] From Tables 1 and 2 and FIG. 1, it can be seen that in Examples 1 to 12 using biochemical instruments (containers) made of a resin composition containing a predetermined cyclic olefin polymer and a predetermined amount of antioxidant, the adsorption of biochemical substances on the contact surface with the biochemical substance sample can be well suppressed even when the biochemical substance is at a low concentration. On the other hand, it can be seen that the biochemical instruments (containers) of Comparative Examples 1 to 8 in which the content of the antioxidant in the resin composition is not within the predetermined range are inferior in the performance of suppressing the adsorption of biochemical substances on the contact surface with the biochemical substance sample. Further, it can be seen that the biochemical instruments (containers) of Comparative Examples 9 and 10 in which the resin composition does not contain a predetermined cyclic olefin polymer are extremely inferior in the performance of suppressing the adsorption of biochemical substances on the contact surface with the biochemical substance sample.

Industrial Applicability

[0085] According to the present invention, it is possible to provide a biochemical instrument capable of well suppressing the adsorption of biochemical substances on the contact surface with the biochemical substance sample.

Claims

1. A biochemical instrument with which a biochemical sample containing nucleic acid comes into contact, wherein the portion with which the biochemical sample comes into contact is made of a resin composition containing at least one cyclic olefin polymer selected from the group consisting of a copolymer of a cyclic olefin and a chain olefin, a ring-opening polymer of a cyclic olefin, and a hydrogenated product of a ring-opening polymer of a cyclic olefin, and a hindered phenol antioxidant, the resin composition contains 0.01 part by mass or more and 0.1 part by mass or less of the hindered phenol antioxidant with respect to 100 parts by mass of the cyclic olefin polymer, the contact angle of the portion with which the biochemical sample comes into contact with water is 90° or more and 95° or less, and the hindered phenol antioxidant contains pentaerythritol tetrakis[3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate], a biochemical instrument.

2. The biochemical instrument according to claim 1, wherein the concentration of the nucleic acid in the biochemical sample is 1000 mg / L or less.

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