Microfluidic chip and microfluidic chip detection system

Abstract

The present disclosure relates to a microfluidic chip and a microfluidic chip detection system. The microfluidic chip includes a storage member with a groove therein, where at least two storage chambers are arranged around the groove, a base arranged at one end of the storage member away from the groove, where a reaction chamber is arranged on the base, and a valve arranged in the groove, where the valve is configured to operatively communicate any one of the at least two storage chambers with the reaction chamber. The present disclosure provides a valve that can be operated to guide a solution in any storage chamber to a reaction chamber, or a solution in a reaction chamber to any storage chamber, thereby realizing the transfer of the solution, making the structure simple and compact, greatly shortening the length of the flow path, and improving the detection efficiency.

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This disclosure is based on and claims priority from Chinese Patent Application No. 202111362356.6, filed on November 17, 2021, the entire contents of which are incorporated herein by reference.

[0002] The present disclosure relates to the field of in vitro diagnostics, and in particular to microfluidic chips and microfluidic chip detection systems. [Background technology]

[0003] Nucleic acid detection technology is a technology that directly detects the genetic material of living organisms, DNA and RNA, and has ultra-high specificity, ultra-high sensitivity, a short detection window, and multiple detection capabilities. However, the nucleic acid detection process is very complex and involves many steps, and it places very high requirements on the detection environment, test chamber conditions, and personnel technical level. Therefore, the development trend of nucleic acid detection is towards fully automated integration, high integration, bedside detection, real-time detection, and random testing at random locations.

[0004] In order to realize the above-mentioned automated integrated detection of nucleic acids, microfluidic technology has emerged in recent years. Microfluidic technology integrates a complex nucleic acid detection process on a chip with micro-sized flow channels and cavities arranged in a regular pattern, releasing different biochemical reagents in a regular sequence and flowing them through different flow channels into designated cavities to complete various biochemical reactions, ultimately achieving rapid and accurate detection of nucleic acids. In this manner, nucleic acid detection based on microfluidic technology has advantages such as full automation, high integration, simplicity, speed, avoidance of cross-contamination, ability to be used independently in various environments, and no need for highly specialized personnel, thereby meeting the concept and requirements of rapid detection. Summary of the Invention [Means for solving the problem]

[0005] In one aspect of the present disclosure, there is provided a microfluidic chip comprising:

[0006] a storage member comprising a groove, the at least two chambers being disposed around the groove;

[0007] a base disposed at one end of the storage member remote from the groove, the reaction chamber being disposed on the base;

[0008] and a valve disposed in the groove, the valve configured to operatively connect any of the at least two storage chambers to the reaction chamber.

[0009] In some embodiments, at least two first storage member inner flow channels are disposed within the storage member, each first storage member inner flow channel correspondingly communicating with one storage chamber, and a valve inner flow channel communicating with the reaction chamber is disposed within the valve, and the valve is configured to operatively communicate the valve inner flow channel with any of the first storage member inner flow channels.

[0010] In some embodiments, a first end of each first storage member inner flow channel penetrates the bottom wall of the groove, the valve is configured to operatively connect the valve inner flow channel to the first end of the first storage member inner flow channel, and the second end of each first storage member inner flow channel connects to the storage chamber via one side of the storage chamber adjacent to the base.

[0011] In some embodiments, the second end of the first storage member inner flow channel communicates with a portion of the storage chamber at a lowest point.

[0012] In some embodiments, both the first end of the valve inner flow channel and the second end of the valve inner flow channel penetrate one end of the valve adjacent the bottom wall of the groove, the first end of the valve inner flow channel is in communication with the reaction chamber, the second end of the valve inner flow channel is in operative communication with any first storage member inner flow channel, the first end of the valve inner flow channel is located in the center of the valve, and the second end of the valve inner flow channel is near the outer edge of the valve.

[0013] In some embodiments, the valve comprises:

[0014] a rotor rotatably disposed within the groove, the rotor including a valve seat and a valve stem, the valve stem being connected to the valve seat;

[0015] a valve cover connected to a peripheral side wall of the groove and abutting against the valve seat to restrict the valve seat between the valve cover and a bottom wall of the groove, the valve cover having a first through hole, an operating portion of the valve shaft passing through the first through hole, the operating portion of the valve shaft being configured to be connected to an external operating member;

[0016] In some embodiments, the valve cover abuts the periphery of the valve seat.

[0017] In some embodiments, the microfluidic chip further includes a sealing film, the at least two storage chambers include reagent chambers, and the sealing film is configured to seal the reagent chambers; the microfluidic chip further includes a top cover and a puncture needle, the top cover is disposed at one end of the storage member having a groove, and the puncture needle is connected to the top cover, and the puncture needle is configured to press the sealing film and puncture the sealing film under the action of an external force.

[0018] In some embodiments, the top cover includes a first rib, the puncture needle is connected to the first rib, and the first rib is configured to be cut under the action of an external force, such that the puncture needle is separated from the top cover and pressed against the sealing film.

[0019] In some embodiments, the central portion of the top cover comprises a second through-hole configured to allow an external operating member to pass therethrough to operate the valve.

[0020] In some embodiments, the puncture needle has a needle inner gas channel therein, and a third through hole that connects the outer portion of the puncture needle with the needle inner gas channel is located near the portion of the puncture needle that connects with the top cover.

[0021] In some embodiments, the microfluidic chip further includes a cover sheet, the cover sheet being disposed within the top cover, the cover sheet having a fourth through-hole through which a puncture needle can penetrate, the puncture needle being configured to press the sealing film under the action of an external force and continue to press the sealing film after puncturing the sealing film, so that the third through-hole is sealed by the cover sheet.

[0022] In some embodiments, the reaction chamber protrudes towards one side away from the storage member.

[0023] In some embodiments, the reaction chamber has a spherical crown structure.

[0024] In some embodiments, the microfluidic chip further includes an amplification member, wherein the amplification member comprises an amplification chamber, a side of the storage member comprises a slot, the slot is located between two adjacent storage chambers, the amplification member is connected to the slot in an insertion manner, and the valve is configured to operatively connect the reaction chamber with the amplification chamber.

[0025] In some embodiments, the storage member comprises a second storage member inner flow channel, a first end of the second storage member inner flow channel extending through the slot and a second end of the second storage member inner flow channel extending through the groove, the amplification member comprises an amplification member inner flow channel in communication with the amplification chamber, the amplification member inner flow channel in communication with the first end of the second storage member inner flow channel, and the valve is in operative communication with the second end of the second storage member inner flow channel and is configured to direct a solution in the reaction chamber through the second storage member inner flow channel and the amplification member inner flow channel to the amplification chamber.

[0026] In some embodiments, the storage member comprises a third storage member inner flow channel, a first end of the third storage member inner flow channel extending through the slot and a second end of the third storage member inner flow channel extending through the groove, the amplification member comprises an amplification member inner gas channel communicating with the amplification chamber, the amplification member inner gas channel communicating with the first end of the third storage member inner flow channel, and the valve is in operative communication with the second end of the third storage member inner flow channel and configured to direct gas in the amplification chamber through the amplification member inner gas channel and the third storage member inner flow channel to the one storage chamber.

[0027] In some embodiments, a valve inner flow channel in communication with the reaction chamber is disposed within the valve, and a valve inner gas channel is also disposed within the valve, and the valve is configured to operatively communicate the valve inner flow channel with the reaction chamber and the amplification chamber, and to communicate the valve inner gas channel with the amplification chamber and one storage chamber.

[0028] In some embodiments, the storage member comprises a storage member inner gas channel, the storage member inner gas channel communicates with the reaction chamber, and the storage member inner gas channel is configured to communicate with an external air pump.

[0029] In some embodiments, the first end of the storage member inner gas channel penetrates one end of the storage member that includes the groove, and the first end of the storage member inner gas channel is located between two adjacent storage chambers.

[0030] In some embodiments, the top cover is fixedly disposed on an end of the storage member that includes the groove, and the base is fixedly disposed on an end of the storage member that is remote from the top cover.

[0031] In one aspect of the present disclosure, there is provided a microfluidic chip detection system including a detection device and the above-mentioned microfluidic chip, wherein the detection device includes an operation table for accommodating the microfluidic chip and an operation member for operating a valve.

[0032] Based on the above technical solutions, the present disclosure has at least the following beneficial effects:

[0033] In some embodiments, the storage member includes a groove, at least two storage chambers are arranged along the circumferential direction of the groove, a valve is arranged in the groove, and a reaction chamber is arranged below the valve. By operating the valve, a solution in any of the storage chambers can be introduced into the reaction chamber, or a solution in the reaction chamber can be introduced into any of the storage chambers, thereby realizing solution transfer, resulting in a simple and compact structure, significantly shortening the length of the flow channel, and improving detection efficiency.

[0034] The accompanying drawings described herein are used to provide a further understanding of the present disclosure and form a part of this application. The exemplary embodiments of the present disclosure and their descriptions are used to explain the present disclosure and do not form undue limitations on the present disclosure. [Brief explanation of the drawings]

[0035] [Figure 1] FIG. 1 is a schematic diagram of the overall structure of a microfluidic chip provided according to some embodiments of the present disclosure.

[0036] [Figure 2] FIG. 2 is a schematic diagram of an exploded structure of a microfluidic chip provided according to some embodiments of the present disclosure.

[0037] [Figure 3] FIG. 3 is a schematic structural diagram of a top cover of a microfluidic chip provided according to some embodiments of the present disclosure.

[0038] [Figure 4] FIG. 4 is a schematic structural diagram of the top cover and cover sheet of the microfluidic chip provided according to some embodiments of the present disclosure.

[0039] [Figure 5] FIG. 5 is a schematic structural diagram of a storage member and a valve of a microfluidic chip provided according to some embodiments of the present disclosure.

[0040] [Figure 6] FIG. 6 is a schematic plan view of a storage member of a microfluidic chip provided according to some embodiments of the present disclosure.

[0041] [Figure 7] FIG. 7 is a schematic bottom view of a storage member of a microfluidic chip provided according to some embodiments of the present disclosure.

[0042] [Figure 8] FIG. 8 is a schematic cross-sectional view of a storage member of a microfluidic chip provided according to some embodiments of the present disclosure.

[0043] [Figure 9] FIG. 9 is a schematic diagram of an exploded structure of a valve of a microfluidic chip provided according to some embodiments of the present disclosure.

[0044] [Figure 10]FIG. 10 is a schematic cross-sectional view of a valve of a microfluidic chip provided according to some embodiments of the present disclosure.

[0045] [Figure 11] FIG. 11 is a structural schematic diagram of the base of a microfluidic chip provided according to some embodiments of the present disclosure.

[0046] [Figure 12] FIG. 12 is a schematic plan view of the base of a microfluidic chip provided in accordance with some embodiments of the present disclosure.

[0047] [Figure 13] FIG. 13 is a schematic diagram of the bottom structure of the base of a microfluidic chip provided according to some embodiments of the present disclosure.

[0048] [Figure 14] FIG. 14 is a schematic cross-sectional view of the base of a microfluidic chip provided according to some embodiments of the present disclosure.

[0049] [Figure 15] FIG. 15 is a schematic diagram of an amplification element of a microfluidic chip provided according to some embodiments of the present disclosure.

[0050] [Figure 16] FIG. 16 is a schematic diagram of a microfluidic chip provided according to some embodiments of the present disclosure before connection of an amplification member, a second gasket, and a storage member.

[0051] [Figure 17] FIG. 17 is a schematic diagram of a microfluidic chip provided according to some embodiments of the present disclosure before insertion of an amplification member into a slot of a storage member. DETAILED DESCRIPTION OF THE INVENTION

[0052] Detailed Description of the Embodiments In the following, the technical solutions in the embodiments are clearly and completely described in combination with the accompanying drawings in the embodiments of the present disclosure. It is clear that the described embodiments are only a part of the embodiments of the present disclosure, and are not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present disclosure without creative work are within the protection scope of the present disclosure.

[0053] In describing the present disclosure, it should be understood that the directions or positional relationships indicated by terms such as "center," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inside," and "outside" are based on the directions or positional relationships shown in the drawings, and are used only for the purpose of facilitating and simplifying the description of the present disclosure, and do not indicate or imply that the specified devices or elements must be specifically disposed, structured, or operated in a particular direction, and therefore should not be understood as a limitation on the protection scope of the present disclosure.

[0054] Some embodiments of the present disclosure provide microfluidic chips and microfluidic chip detection systems suitable for rapid in vitro detection.

[0055] As shown in FIGS. 1 and 2, in some embodiments, a microfluidic chip is provided that includes a storage member 1, a base 2, and a valve 3.

[0056] 5 and 6, the storage member 1 has a groove 11 therein, around which at least two storage chambers 12 are arranged. The groove 11 includes a bottom wall and a peripheral side wall.

[0057] As shown in FIGS. 1 and 2, a base 2 is disposed at one end of the storage member 1 remote from the groove 11 , and a reaction chamber 21 is disposed on the base 2 .

[0058] As shown in FIGS. 1, 2 and 5, the valve 3 is disposed within the groove 11 and is configured to place any one of the at least two storage chambers 12 in operative communication with the reaction chamber 21.

[0059] The at least two storage chambers 12 include at least one sample chamber and at least two reagent chambers 121. The reagent chambers 121 are used to store different detection reagents, which may be solid or liquid, and the number of reagent chambers 121 can be flexibly increased or decreased as needed. One sample chamber may be provided, or two may be provided as needed. The sample chambers are used to add a sample to be detected, such as blood or saliva.

[0060] The reaction chamber 21 is a reagent reaction space for nucleic acid extraction.

[0061] The valve 3 is an important component for fluid control within the microfluidic chip, and controls the opening and closing of the flow channel.

[0062] The base 2 is for stably and flatly laying the microfluidic chip, and has the functions of positioning and limiting, thereby improving the stability of detection.

[0063] According to an embodiment of the present disclosure, a groove 11 is disposed in a storage member 1, at least two storage chambers 12 are arranged along the circumferential direction of the groove 11, a valve 3 is disposed in the groove 11, and a reaction chamber 21 is disposed below the valve 3. By operating the valve 3, a solution in any of the storage chambers 12 can be introduced into the reaction chamber 21, or a solution in the reaction chamber 21 can be introduced into any of the storage chambers 12, thereby realizing the transfer of solutions, resulting in a simple and compact structure, significantly shortening the length of the flow channel, and improving detection efficiency.

[0064] As shown in Figure 8, at least two first storage member inner flow channels 13 are disposed within the storage member 1, and each first storage member inner flow channel 13 is in communication with one corresponding storage chamber 12. As shown in Figure 10, a valve inner flow channel 31 in communication with the reaction chamber 21 is disposed within the valve 3, and the valve 3 is configured to operatively connect the valve inner flow channel 31 with any one of the first storage member inner flow channels 13.

[0065] The valve inner flow channel 31 is always in communication with the reaction chamber 21. By operating the valve 3, the valve inner flow channel 31 is selectively in communication with any one of the first storage member inner flow channels 13 to introduce the solution in the storage chamber 12 into the reaction chamber 21 or to introduce the solution in the reaction chamber 21 into the storage chamber 12.

[0066] In some embodiments, as shown in Figures 6 to 8, the first end 131 of each first storage member inner flow channel 13 penetrates the bottom wall of the groove 11, the valve 3 is configured to operatively connect the valve inner flow channel 31 to the first end 131 of the first storage member inner flow channel 13, and the second end 132 of each first storage member inner flow channel 13 connects to the storage chamber 12 via one side of the storage chamber 12 adjacent to the base 2.

[0067] In some embodiments, as shown in Figures 6-8, the second end 132 of the first storage member inner flow channel 13 communicates with the portion of the storage chamber 12 closest to the groove 11 at its lowest point.

[0068] In some embodiments, as shown in Figures 6-8, the second end 132 of the first storage member inner flow channel 13 communicates with the portion of the storage chamber 12 closest to the groove 11 at its lowest point, shortening the length of the flow channel.

[0069] In some embodiments, as shown in Figures 9 and 10, both the first end 311 and the second end 312 of the valve inner flow channel 31 penetrate one end of the valve 3 adjacent to the bottom wall of the groove 11, the first end 311 of the valve inner flow channel 31 communicates with the reaction chamber 21, the second end 312 of the valve inner flow channel 31 operatively communicates with any one of the first storage member inner flow channels 13, the first end 311 of the valve inner flow channel 31 is located in the center of the valve 3, and the second end 312 of the valve inner flow channel 31 is near the outer edge of the valve 3.

[0070] In some embodiments, as shown in FIGS. 5 and 8, the storage member 1 is generally cylindrical.

[0071] In some embodiments, as shown in FIGS. 2, 9 and 10, the valve 3 includes a rotor 32 and a valve cover 33.

[0072] The rotor 32 is rotatably disposed in the groove 11. The rotor 32 includes a valve seat 321 and a valve shaft 322, and the valve shaft 322 is connected to the valve seat 321.

[0073] The valve cover 33 is connected to the peripheral side wall of the groove 11 and abuts against the valve seat 321, which is limited between the valve cover 33 and the bottom wall of the groove 11 and abuts against the bottom wall of the groove 11. The valve cover 33 has a first through-hole 331, and the operating portion of the valve shaft 322 passes through the first through-hole 331, and the operating portion of the valve shaft 322 is configured to be connected to an external operating member.

[0074] The valve seat 321 includes a valve seat body 3211 and a first gasket 3212. The first gasket 3212 is shaped to match the bottom of the valve seat body 3211, and the valve seat body 3211 and the first gasket 3212 are fixedly arranged. The valve inner flow channel 31 is formed within the valve seat 321. The valve seat body 3211 is made of a hard material, and the first gasket 3212 is made of an elastic material. By adjusting the valve cover 33, an appropriate contact pressure is applied to the valve seat 321 of the rotor 32, so that the valve seat 321 contacts the bottom wall of the groove 11. Furthermore, the valve inner flow channel 31 has the required airtightness and prevents liquid leakage at the junction between the valve inner flow channel 31 and the storage member inner flow channel.

[0075] In some embodiments, the valve cover 33 abuts the periphery of the valve seat 321 .

[0076] The first end 131 of each first storage member inner flow channel 13 penetrates the bottom wall of the groove 11 and is arranged around the central axis of the groove 11, and the valve cover 33 abuts against the periphery of the valve seat 321, so that the valve seat 321 can abut against the bottom wall of the groove 11, and the junction between the valve inner flow channel 31 and the storage member inner flow channel is sealed to avoid liquid leakage.

[0077] The radial size of the valve shaft 322 is smaller than the radial size of the valve seat body 3211, and one end of the valve shaft 322 is fixedly connected to the valve seat body 3211, while the other end of the valve shaft 322 is an operating part for connecting to an external operating member by passing through the first through-hole 331. The valve shaft 322 is rotated by the operating member, which further rotates the valve seat body 3211 and the first gasket 3212, and selectively connects the second end 312 of the valve inner flow channel 31 to one of the first storage member inner flow channels 13.

[0078] Optionally, the operating portions of the valve stem 322 are arranged in a hexagonal or square configuration.

[0079] The boss 324 is arranged circumferentially on the valve seat body 3211, and the boss 324 has an arcuate contour so as to reduce friction between the circumferential sidewall of the valve seat body 3211 and the circumferential sidewall of the groove 11 during rotation of the valve seat body 3211 relative to the groove 11.

[0080] 3 to 5, the microfluidic chip further includes a sealing film, and the at least two storage chambers 12 include reagent chambers 121, and the sealing film is configured to seal the reagent chambers 121. The microfluidic chip further includes a top cover 4 and a puncture needle 41. The top cover 4 is disposed at one end of the storage member 1 having the groove 11, and the puncture needle 41 is connected to the top cover 4, and the puncture needle 41 is configured to press the sealing film and puncture the sealing film under the action of an external force.

[0081] In some embodiments, as shown in Figures 3 to 5, the top cover 4 includes a first rib 421, the puncture needle 41 is connected to the first rib 421, and the first rib 421 is configured to be cut under an external force, resulting in the puncture needle 41 being separated from the top cover 4 and pressed against the sealing film.

[0082] In some embodiments, as shown in Figures 3 to 5, the central portion of the top cover 4 has a second through-hole 43, which is configured to allow an external operating member to pass through to connect with the operating portion of the valve 3 in order to rotate the valve 3.

[0083] In some embodiments, as shown in FIG. 4, the puncture needle 41 has a needle inner gas channel 411 therein, and the portion of the puncture needle 41 that connects to the top cover 4 has a third through hole 412, which connects the outer portion of the puncture needle 41 with the needle inner gas channel 411.

[0084] 4 , the microfluidic chip further includes a cover sheet 6, which is disposed within the top cover 4 and includes a sixth through-hole 62 through which an external operating member can penetrate to connect the rotor 32. The cover sheet 6 also includes a fourth through-hole 61 through which the puncture needle 41 can penetrate. The puncture needle 41 is configured to press the sealing film under the action of an external force and continue to press the sealing film even after puncturing the sealing film, so that the third through-hole 412 is sealed by the cover sheet 6.

[0085] 4, the puncture needle 41 includes a first needle segment and a second needle segment, and the radial size of the second needle segment is larger than the radial size of the first needle segment. The second needle segment is connected to the first rib 421. The first needle segment is configured as a sharp needle for puncturing the sealing film, and the third through-hole 412 is disposed in the second needle segment.

[0086] The puncture needle is pressed against the sealing film under the action of an external force, puncturing the sealing film, so that the reagent chamber 121 communicates with the atmosphere through the needle inner gas channel 411 and the third through-hole 412 of the puncture needle 41, which is convenient for subsequent reagent extraction. At this time, the first needle segment penetrates the fourth through-hole 61. After detection, the puncture needle 41 is further pressed against the sealing film under the action of an external force, and the third through-hole 412 is sealed by the cover sheet 6 to seal the reagent chamber 121 to prevent waste liquid from leaking.

[0087] In some embodiments, a sealing film is placed on the cover sheet 6 to seal the reagent chamber 121 .

[0088] In some other embodiments, the sealing film is placed directly on the reagent chamber 121 to seal the reagent chamber 121 .

[0089] In some embodiments, the reaction chamber 21 protrudes towards one side away from the storage member 1, as shown in FIGS.

[0090] In some embodiments, the reaction chamber 21 has a spherical-cap structure, as shown in Figures 11 to 14. As a reagent reaction field for nucleic acid extraction, the reaction chamber 21 completes the extraction step in a short time by cooperation between the spherical-cap cavity structure and the ultrasonic transducer.

[0091] 15 to 17, the microfluidic chip further includes an amplification member 5, which includes an amplification chamber 51. As shown in FIGS. 5 and 6, a side of the storage member 1 includes a slot 14, which is located between two adjacent storage chambers 12, the amplification member 5 is connected to the slot 14 in an insertion manner, and the valve 3 is configured to operatively connect the reaction chamber 21 to the amplification chamber 51.

[0092] The amplifying member 5 has a thin plate structure with a large heating surface, making it easy to collect fluorescence from the sample.

[0093] The amplifying member 5 is designed as a separate body, and can be connected to or separated from the main body structure (including the storage member 1) of the microfluidic chip by inserting or removing it, thereby improving versatility.

[0094] In some embodiments, as shown in FIGS. 5-8 , the storage member 1 includes a second storage member inner flow channel 15, a first end 151 of the second storage member inner flow channel 15 extending through the slot 14, and a second end 152 of the second storage member inner flow channel 15 extending through the groove 11. As shown in FIG. 15 , the amplification member 5 includes an amplification member inner flow channel 52 communicating with the amplification chamber 51, the amplification member inner flow channel 52 communicating with the first end 151 of the second storage member inner flow channel 15. The valve 3 is configured to be in operative communication with the second end 152 of the second storage member inner flow channel 15, and directs the solution in the reaction chamber 21 through the second storage member inner flow channel 15 and the amplification member inner flow channel 52 to the amplification chamber 51.

[0095] In some embodiments, as shown in FIGS. 5-8 , the storage member 1 includes a third storage member inner flow channel 16, where a first end 161 of the third storage member inner flow channel 16 passes through the slot 14 and a second end 162 of the third storage member inner flow channel 16 passes through the groove 11. As shown in FIG. 15 , the amplification member 5 includes an amplification member inner gas channel 53 in communication with the amplification chamber 51, where the amplification member inner gas channel 53 is in communication with the first end 161 of the third storage member inner flow channel 16. The valve 3 is configured to direct gas in the amplification chamber 51 through the amplification member inner gas channel 53 and the third storage member inner flow channel 16 to one storage chamber 12 and to maintain air pressure balance within the amplification chamber 51, and is in operative communication with the second end 162 of the third storage member inner flow channel 16. When the amount of sample to be detected is too large to fit in the amplification chamber 51, the excess liquid in the amplification chamber 51 can also be discharged to the storage chamber 12 through the above-mentioned connected gas channel and flow channel to avoid leakage and contamination. The amplification member 5 includes a main body part and an insertion / removal part, the amplification chamber 51 is disposed in the main body part, and the insertion / removal part is aligned with and connected to the slot 14 in an insertion / removal manner.

[0096] Both the amplification member inner flow channel 52 and the amplification member inner gas channel 53 communicate with the amplification chamber 51, and a portion of the amplification member inner flow channel 52 and the amplification member inner gas channel 53 is disposed in the insertion / removal portion.

[0097] The valve 3 communicates with the second end 152 of the second storage member inner flow channel 15 to introduce the solution in the reaction chamber 21 through the second storage member inner flow channel 15 and the amplification member inner flow channel 52 into the amplification chamber 51, while the valve 3 also communicates with the second end 162 of the third storage member inner flow channel 16 to introduce the gas in the amplification chamber 51 through the amplification member inner gas channel 53 and the third storage member inner flow channel 16 into one storage chamber 12. Optionally, the storage chamber 12 is adjacent to the slot 14.

[0098] 9 and 10, the valve 3 includes a valve inner flow channel 31 therein that communicates with the reaction chamber 21. The valve 3 also includes a valve inner gas channel 34 therein, and the valve 3 is configured to operatively communicate the valve inner flow channel 31 with the reaction chamber 21 and the amplification chamber 51, and to communicate the valve inner gas channel 34 with the amplification chamber 51 and one of the storage chambers 12.

[0099] 5 to 7, the storage member 1 includes a storage member inner gas channel 17, which communicates with the reaction chamber 21, and which is configured to communicate with an external air pump. The air pump provides suction force for introducing the solution in the storage chamber 12 into the reaction chamber 21 through the valve 3, or provides blowing force for introducing the solution in the reaction chamber 21 into the storage chamber 12 through the valve 3.

[0100] In some embodiments, as shown in Figures 5 to 7, the first end 171 of the storage member inner gas channel 17 penetrates one end of the storage member 1 having the groove 11, and the first end 171 of the storage member inner gas channel 17 is located between two adjacent storage chambers 12.

[0101] In some embodiments, as shown in FIG. 1, the top cover 4 is fixedly positioned at one end of the storage member 1 having the groove 11, and the base 2 is fixedly positioned at one end of the storage member 1 remote from the top cover 4.

[0102] 2, the storage member 1 has a cylindrical structure, the cover surface of the top cover 4 covering the storage member 1 is circular, the surface of the base 2 connected to the storage member 1 is circular, and the cover sheet 6 is circular. The groove 11 disposed in the storage member 1 is a cylindrical groove. The base 321 of the rotor 32 of the valve 3 and the first gasket 3212 are circular.

[0103] Some specific embodiments of the microfluidic chip will be described in detail with reference to FIGS.

[0104] As shown in FIGS. 1 and 2, the microfluidic chip includes a storage member 1, a base 2, a valve 3, a top cover 4, an amplification member 5 and a cover sheet 6.

[0105] As shown in FIGS. 1 and 2, the top cover 4 is fixed to the top of the storage member 1 and encloses a portion of the top of the storage member 1 therein, and a cover sheet 6 is disposed between the top cover 4 and the top of the storage member 1. The base 2 is fixedly disposed on the bottom of the storage member 1. The central position of the top of the storage member 1 is provided with a groove 11 recessed toward the bottom, the valve 3 is disposed in the groove 11, and the operating portion of the valve 3 extends from the groove 11 toward the top cover 4. The top cover 4 is provided with a through-hole through which the operating portion of the valve 3 can pass, or an external operating member extends into the through-hole to connect with the operating portion of the valve 3 and operate the valve 3. The amplifying member 5 is disposed on the side of the storage member 1 in an insertable / removable manner, and the side of the top cover 4 is provided with a notch to avoid the amplifying member 5.

[0106] As shown in FIGS. 2 to 4, the top cover 4 includes a circular cover plate 47, the central portion of which is provided with a second through-hole 43 through which the operating portion of the valve 3 passes, or an external operating member extends into the second through-hole 43 to be connected to the operating portion of the valve 3. An annular peripheral side wall 48 extending to the storage member 1 is disposed in the circumferential direction of the cover plate 47, and the peripheral side wall 48 of the top cover 4 encloses a portion of the top of the storage member 1 therein. The peripheral side wall 48 of the top cover 4 is provided with a clamp block, and the top of the storage member 1 is provided with a clamp groove, so that the top cover 4 and the storage member 1 are fixedly connected via the structure of the clamp block and the clamp groove.

[0107] The cover plate 47 of the top cover 4 has a sample addition port 46, which corresponds to the position of one of the multiple storage chambers 12, and this storage chamber 12 functions as the sample addition chamber, and the sample to be detected is added to the sample addition chamber through the sample addition port 46.

[0108] The cover plate 47 of the top cover 4 also has a fifth through-hole 44, which is used to communicate with the storage member inner gas channel 17 of the storage member 1, which communicates with the reaction chamber 21, and the gas in the reaction chamber 21 communicates with the outside through the storage member inner gas channel 17 and the fifth through-hole 44. The fifth through-hole 44 may function as a pump interface and can be connected to an air pump, which provides suction force to introduce the solution in the storage chamber 12 into the reaction chamber 21 through the valve 3, and also provides blowing force to introduce the solution in the reaction chamber 21 into the storage chamber 12 through the valve 3.

[0109] The storage member 1 includes a plurality of storage chambers 12 around the groove 11. Accordingly, a plurality of puncture needles 41 are connected to the cover plate 47 of the top cover 4 in a corresponding manner, all of which are spaced apart around the center line of the groove 11, and each puncture needle 41 corresponds to one storage chamber 12. Each puncture needle 41 may be connected to an annular member 45, the outer edge of which may be connected to the cover plate of the top cover 4 via a plurality of first ribs 421, and the inner edge of which may be connected to the cylindrical member 49 via a plurality of second ribs 422.

[0110] The puncture needle 41 has a hollow structure, i.e., a needle-inner gas channel 411 therein, and also a third through-hole 412 that connects the needle-inner gas channel 411 to the outside atmosphere. The puncture needle 41 includes a first needle segment and a second needle segment, and the radial size of the second needle segment is larger than the radial size of the first needle segment. The second needle segment is connected to the annular member 45, and the first needle segment is configured as a sharp needle for puncturing the sealing film, and the third through-hole 412 is disposed in the second needle segment.

[0111] When the microfluidic chip is used, pressure is applied to the annular member 45 of the top cover 4 to break the first rib 421, causing the annular member 45 to separate all of the puncture needles 41 from the cover plate 47 and press them against the sealing film of the storage chamber 12, causing the puncture needles 41 to pierce the sealing film, and the cylindrical member 49 abuts against the peripheral side wall of the groove 11 to prevent the puncture needles 41 from descending excessively. At this time, the gas in the storage chamber 12 communicates with the atmosphere through the needle-inner gas channel 411 and the third through-hole 412. After the extraction step is completed, an external force is continuously applied to the annular member 45 and all the puncture needles 41, causing the second rib 422 to break, the annular member 45 to separate from the cylindrical member 49, the cylindrical member 49 to no longer hinder the downward movement of the puncture needles 41, and the annular member 45 and all the puncture needles 41 to be further pressed against the sealing film under the action of the external force, causing the second needle segment to interference-fit into the fourth through-hole 61. The second needle segment aligns with the cover sheet 6 to block the third through-hole 412 of the second needle segment, sealing the storage chamber 12 and preventing leakage of the waste liquid in the storage chamber 12.

[0112] The cover sheet 6 is circular and has a fourth through hole 61, a sixth through hole 62, and a seventh through hole 63 in its center. The sixth through hole 62 is aligned with the second through hole 43 of the cover plate 47, and is used for allowing the operating portion of the valve 3 to pass through, or for allowing an external operating member to extend into the sixth through hole 62 and connect to the operating portion of the valve 3. There are multiple fourth through holes 61, and each fourth through hole 61 corresponds to one puncture needle 41. The seventh through hole 63 is aligned with the fifth through hole 44 of the top cover 4 so as to communicate with the storage member inner gas channel 17.

[0113] The radial size of the second needle segment of the puncture needle 41 is larger than the radial size of the first needle segment. When the microfluidic chip is used, the first needle segment punctures the sealing film through the fourth through-hole 61, and the second needle segment and the third through-hole 412 are located above the cover sheet 6. After the extraction step is completed, the annular member 45 and each puncture needle 41 are further pressed against the sealing film under the action of an external force, and the second needle segment is interference-fitted into the fourth through-hole 61. The second needle segment aligns with the cover sheet 6 to block the third through-hole 412 and seal the storage chamber 12, preventing leakage of waste liquid in the storage chamber 12.

[0114] In summary, the puncture needle 41 of the top cover 4 is used to puncture the sealing film of the storage chamber 12 and connect the storage chamber 12 to the atmosphere. The cover sheet 6 is used to align with the puncture needle 41 of the top cover 4 to seal the storage chamber 12 after detection.

[0115] As shown in FIGS. 5 to 8 , the storage member 1 is cylindrical and has a groove 11 at the center of its top end, recessed toward the bottom. Multiple storage chambers 12 are arranged around the groove 11. The storage chambers 12 can serve as both sample addition chambers and reagent chambers 121. In this embodiment, the multiple storage chambers 12 include one sample addition chamber and multiple reagent chambers 121. The sample addition chamber is used to add a sample to be detected, and the reagent chambers 121 contain reagents for biochemical reactions. The top and bottom surfaces of the reagent chambers 121 are sealed with a sealing film. The second end 132 of the first storage member inner flow channel 13 communicates with the reagent chambers 121 through the lowest position of the reagent chambers 121. To ensure convenient sealed packaging, transportation, and storage of the reagents, an appropriate connection method can be selected for the reagent chambers 121 as needed. The storage chambers 12 have an oval cross section. The cross section of the storage chambers 12 is narrower near the centerline of the groove 11 and wider away from the centerline of the groove 11. The size and distribution of the storage chambers 12 can be adjusted as needed.

[0116] The storage member 1 includes a storage member inner gas channel 17 therein, and a first end of the storage member inner gas channel 17 is located between two adjacent storage chambers 12. The storage member inner gas channel 17 communicates with the reaction chamber 21. The side of the storage member 1 includes a slot 14 for inserting the amplification member 5.

[0117] The storage member 1 has at least two first storage member inner flow channels 13 therein, each of which communicates with one corresponding storage chamber 12. The first end 131 of each first storage member inner flow channel 13 penetrates the bottom wall of the groove 11 to communicate with the valve inner flow channel 31 of the valve 3. The second end 132 of each first storage member inner flow channel 13 communicates with the storage chamber 12 through one side of the storage chamber 12 adjacent to the base 2, and the second end 132 of the first storage member inner flow channel 13 communicates with the lowest part of the storage chamber 12 to prevent reagent residue. The second end 132 of the first storage member inner flow channel 13 communicates with the storage chamber 12 closest to the groove 11, shortening the communication distance with the valve 3 and improving detection efficiency.

[0118] The storage member 1 has a fourth storage member inner flow channel 18 therein, and a first end 181 of the fourth storage member inner flow channel 18 penetrates the bottom wall of the groove 11 and is located in the center of the groove 11, and the first end 181 of the fourth storage member inner flow channel 18 is used to communicate with the valve inner flow channel 31 of the valve 3. The second end of the fourth storage member inner flow channel 18 communicates with the reaction chamber 21.

[0119] The storage member 1 has a second storage member inner flow channel 15, a first end 151 of the second storage member inner flow channel 15 passing through the slot 14 to communicate with the amplification member inner flow channel 52, and a second end 152 of the second storage member inner flow channel 15 passing through the groove 11 to communicate with the valve inner flow channel 31 of the valve 3.

[0120] The storage member 1 includes a third storage member inner flow channel 16, a first end 161 of which passes through the slot 14 to communicate with the amplification member inner gas channel 53. A second end 162 of the third storage member inner flow channel 16 passes through the groove 11 to communicate with the valve inner gas channel 34 of the valve 3.

[0121] The slot 14 includes a first buckle 141 for mating with and connecting to a second buckle 54 on the amplifying member 5 .

[0122] 9 and 10, the valve 3 is used to control the closure of the liquid passages and to communicate with each chamber. The valve 3 includes a rotor 32 and a valve cover 33.

[0123] The valve cover 33 is used to connect with the peripheral side wall of the groove 11, and the valve cover 33 has a first through hole 331, and the operating part of the rotor 32 passes through the first through hole 331, and the operating part of the rotor 32 is configured to connect with an external operating member. The valve cover 33 has a plurality of connection blocks connected to the peripheral side wall of the groove 11.

[0124] The rotor 32 is rotatably disposed in the groove 11. The rotor 21 includes a valve seat body 3211, a valve stem 322, and a first gasket 3212. The bottoms of the first gasket 3212 and the valve seat body 3211 are identical in shape and are both circular. The valve seat body 3211 and the first gasket 3212 are fixedly disposed. The valve inner flow channel 31 is formed within the combined structure of the valve seat body 3211 and the first gasket 3212.

[0125] The radial size of the valve shaft 322 is smaller than the radial size of the valve seat body 3211, one end of the valve shaft 322 is fixedly connected to the valve seat body 3211, and the other end of the valve shaft 322 passes through the first through-hole 331 and is an operating part for connecting to an external operating member.

[0126] The valve 3 comprises a valve inner flow channel 31 and a valve inner gas channel 34 .

[0127] The first end 311 of the valve inner flow channel 31 is located in the center of the valve 3 and is aligned with and communicates with the first end 181 of the fourth storage member inner flow channel 18 in the groove 11. The valve inner flow channel 31 always communicates with the reaction chamber 21 through the fourth storage member inner flow channel 18, and the second end 312 of the valve inner flow channel 31 selectively communicates with either the first storage member inner flow channel 13 or the second storage member inner flow channel 15 while rotating with the rotor 32, and the second end 312 of the valve inner flow channel 31 is near the outer edge of the valve 3.

[0128] When the second end 312 of the valve inner flow channel 31 is connected to the second storage member inner flow channel 15, the first end of the valve inner gas channel 34 is connected to the second end 162 of the third storage member inner flow channel 16, the second end of the valve inner gas channel 34 is connected to the first end 131 of the first storage member inner flow channel 13, and the second end 132 of the first storage member inner flow channel 13 is connected to one storage chamber 12.

[0129] The operating part of the valve shaft 322 is connected via an external operating member, and when the valve shaft 322 is rotated, the valve seat body 3211 and the first gasket 3212 are further rotated, so that the second end 312 of the valve inner flow channel 31 is selectively connected to one of the first storage member inner flow channel 13 or the second storage member inner flow channel 15, thereby completing the movement of the liquid through the flow channel during the detection process.

[0130] Optionally, the operating portion of the valve stem 322 is configured in a hexagonal configuration.

[0131] The circumferential direction of the valve seat body 3211 is provided with a boss 324, which has an arcuate contour so as to reduce friction between the circumferential direction of the valve seat body 3211 and the circumferential side wall of the groove 11 during rotation of the valve seat body 3211 relative to the groove 11.

[0132] As shown in FIGS. 11 to 14, the base 2 includes a chassis 22, a support member 23, and a positioning member 24.

[0133] The surface of the chassis 22 is circular, and the chassis 22 is provided with positioning lugs 27, which are used to connect the storage member 1.

[0134] The support member 23 is disposed below the chassis 22 to support the chassis 22 and the entire microfluidic chip. As a support structure for the microfluidic chip, the support member 23 allows the microfluidic chip to be stably positioned. The bottom of the support member 23 is provided with a positioning groove 28, which is also used to complete the initial positioning of the microfluidic chip by aligning it with a mounting base on the detection device. A clamping groove 25 is formed between the support member 23 and the chassis 22. During the process of pressing the microfluidic chip into the detection device, the clamping groove 25 is used to align and position the microfluidic chip with a structure on the detection device to further fix it, thereby avoiding detection errors due to movement of the microfluidic chip during the detection process and improving the consistency of detection.

[0135] The reaction chamber 21 is located at the bottom of the chassis 22, protruding downward and having a spherical cap structure. This structure can be coupled with an ultrasonic head to achieve rapid resonance and promote sample lysis and magnetic bead mixing. The chassis 22 includes a chassis inner flow channel communicating with the reaction chamber 21. A first end 261 of the chassis inner flow channel is located in the center of the chassis 22, and a second end 262 of the chassis inner flow channel communicates with the reaction chamber 21. The first end 261 of the chassis inner flow channel communicates with the fourth storage member inner flow channel 18 and is aligned with the first end 311 of the valve inner flow channel 31, so that the valve inner flow channel 31 always communicates with the reaction chamber 21. The second end 262 of the chassis inner flow channel communicates with the reaction chamber 21 through the lowest part of the reaction chamber 21, preventing the formation of a dead zone that would prevent reagents from being completely discharged.

[0136] A positioning member 24 is disposed on the support member 23 for positioning the microfluidic chip when it is placed on the detection device.

[0137] The above-mentioned top cover 4 is fixedly disposed on one end of the storage member 1 having a groove 11, the base 2 is fixedly disposed on one end of the storage member 1 remote from the top cover 4, and the valve 3 is disposed in the groove 11. The top cover 4, the storage member 1, the base 2 and the valve 3 form the main structure of the microfluidic chip.

[0138] 15 to 17, the amplifying member 5 has a sheet structure for realizing amplification of rapid temperature increase and decrease. The amplifying member 5 is attached to and detached from the slot 14 of the storage member 1 by inserting and removing it. The amplifying member 5 may be separated from the main structure of the microfluidic chip, or may be made of a material different from the main structure and joined thereto.

[0139] The amplification chamber 51 is disposed within the amplification member 5, and the amplification chamber 51 is sheet-shaped, which allows for a larger contact surface area with the heat source and improved thermal conductivity. The amplification member 5 includes an amplification member inner flow channel 52 communicating with the amplification chamber 51, and the amplification member inner flow channel 52 communicates with a first end 151 of the second storage member inner flow channel 15. The amplification member 5 includes an amplification member inner gas channel 53 communicating with the amplification chamber 51, and the amplification member inner gas channel 53 communicates with a first end 161 of the third storage member inner flow channel 16.

[0140] The joint between the main body structure of the microfluidic chip and the amplification member 5 may be fixed with a second gasket 7 made of soft rubber using secondary injection molding or adhesive to ensure airtightness of the amplification member inner flow channel 52 and the amplification member inner gas channel 53 at the joint.

[0141] The cross section of the first buckle 141 disposed in the slot 14 and the cross section of the second buckle 54 disposed in the amplifying member 5 may both be triangular. After the amplifying member 5 is inserted into the slot 14, the first buckle 141 and the second buckle 54 mutually restrict the amplifying member 5 from being pulled out of the slot 14.

[0142] Some embodiments also provide a microfluidic chip detection system including a detection device and the above-described microfluidic chip, wherein the detection device includes an operation table for accommodating the microfluidic chip and an operation member for operating the valve 3.

[0143] The microfluidic chip detection system according to the embodiment of the present disclosure places low demands on the operator; the detection process, including extraction and amplification, can be initiated simply by adding the sample to be detected, inserting the microfluidic chip into the detection device, and clicking the start button.

[0144] The detection flow of the microfluidic chip will be explained below.

[0145] Depending on the detection item, a microfluidic chip loaded with a corresponding reagent is selected, and the sample to be detected is injected into the sample chamber of the microfluidic chip, completing the preliminary preparation.

[0146] The operator takes note of the base 2 of the microfluidic chip and the corresponding positioning structure of the detection device, places the base 2 of the microfluidic chip flat on the tray of the detection device, and completes the initial positioning. By clicking the start button, the tray enters the working area of ​​the detection device, and the clamping grooves 25 of the base 2 align with the positioning structure of the detection device, clamping the microfluidic chip.

[0147] At the start of the detection process, the air pump is connected to the air pump interface in the microfluidic chip, and the puncture needle 41 on the top cover 4 of the microfluidic chip is pressed downward, causing the first rib 421 to break, thereby separating the puncture needle 41 from the top cover 4 and puncturing the sealing film of the storage chamber 12, and the storage chamber 12 is connected to the atmosphere through the needle inner gas channel 411 and the third through hole 412 of the puncture needle 41, preparing for the release of the reagent.

[0148] The valve 3 is rotated to connect with one storage chamber 12 and one reaction chamber 21 respectively, and the power source is supplied by an external air pump, so that the reagent extraction required by each extraction step is completed in order.

[0149] In this embodiment, the magnetic bead method is used for nucleic acid extraction. After the reagent from the extraction storage chamber 12 enters the reaction chamber 21, the ultrasonic head is coupled to the reaction chamber 21 through resonance. The spherical cap structure of the reaction chamber 21 provides better support, preventing wall deformation during the ultrasonic process and improving contact surface consistency. Under the action of ultrasound, the wall vibrates, stirring the reagent and magnetic beads in the reaction chamber 21. This completes the sample dissolution and uniform mixing of the magnetic beads within a few seconds. After the reaction, the waste liquid is returned from the reaction chamber 21 to the storage chamber 12, which is then sealed by rotating the valve 3.

[0150] After the reagents are sequentially extracted and reacted in the reaction chamber 21, a purified extract is finally obtained, and the purified extract is transferred to the amplification chamber 51, which is then sealed by rotating the valve 3, waiting for the amplification module of the detection device to perform rapid amplification of the sample and multi-flow channel optical detection.

[0151] After the detection is completed, the pressure module in the detection device again acts on the puncture needle 41 on the top cover 4 of the microfluidic chip, causing the second rib 422 to break under the pressure, and the puncture needle 41 continues to move downward. The second needle segment of the puncture needle 41 forms an interference fit with the cover sheet 6, and the cover sheet 6 covers the third through-hole 412 of the second needle segment, thereby isolating the storage chamber 12 from the atmosphere and preventing the reaction waste liquid in the storage chamber 12 from leaking.

[0152] At this point, the detection flow of the microfluidic chip is completely finished, and the user can click the "Exit Chamber" button to release the pressure, remove the microfluidic chip, and start detecting the next group.

[0153] The flow channels of the present disclosure can be used for liquid and gas transmission, and similarly, the gas channels can be used for gas and liquid transmission.

[0154] Based on each of the above embodiments of the present disclosure, the technical features of one of the embodiments may be beneficially combined with one or more of the other embodiments without explicit negation or conflict.

[0155] Finally, it should be noted that the above embodiments are only used to illustrate, not to limit, the technical solutions of the present disclosure. That is, although the present disclosure has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can be made to the specific embodiments of the present disclosure or equivalent substitutions can be made to some of the technical features. And, without departing from the spirit of the technical solutions of the present disclosure, the modifications and equivalent substitutions should be included in the scope of the technical solutions claimed in the present disclosure. [Configuration 1] A microfluidic chip comprising: a storage member (1) having a groove (11) therein and at least two storage chambers (12) arranged around said groove (11); a base (2) disposed at one end of the storage member (1) remote from the groove (11), the reaction chamber (21) being disposed on the base (2); a valve (3) disposed within the groove (11), the valve (3) being configured to operatively connect any one of the at least two storage chambers (12) to the reaction chamber (21). [Configuration 2] At least two first storage member inner flow channels (13) are disposed within the storage member (1); Each first storage member inner flow channel (13) is in communication with one corresponding storage chamber (12); A valve inner flow channel (31) communicating with the reaction chamber (21) is disposed within the valve (3); and 2. The microfluidic chip of claim 1, wherein the valve (3) is configured to operatively connect the valve inner flow channel (31) with any first storage member inner flow channel (13). [Configuration 3] a first end (131) of each first storage member inner flow channel (13) penetrates the bottom wall of said groove (11); the valve (3) is configured to place the valve inner flow channel (31) in operative communication with the first end (131) of the first storage member inner flow channel (13); and A microfluidic chip as described in configuration 2, wherein the second end (132) of each first storage member inner flow channel (13) is connected to the storage chamber (12) through one side of the storage chamber (12) adjacent to the base (2). [Configuration 4] A microfluidic chip as described in configuration 3, wherein the second end (132) of the first storage member inner flow channel (13) communicates with a portion of the storage chamber (12) at its lowest point. [Configuration 5] a first end (311) of the valve inner flow channel (31) and a second end (312) of the valve inner flow channel (31) both pass through one end of the valve (3) adjacent to the bottom wall of the groove (11); the first end (311) of the valve inner flow channel (31) communicates with the reaction chamber (21); the second end (312) of the valve inner flow channel (31) is in operative communication with any first storage member inner flow channel (13); The first end (311) of the valve inner flow channel (31) is located in the center of the valve (3), and 5. The microfluidic chip of configuration 3 or 4, wherein the second end (312) of the valve inner flow channel (31) is near the outer edge of the valve (3). [Configuration 6] The valve (3) a rotor (32) rotatably disposed within the groove (11), The rotor (32) includes a valve seat (321) and a valve stem (322). The valve stem (322) is connected to the valve seat (321), and the rotor (32) is connected to the valve seat (321); A valve cover (33), a valve cover (33) connected to a peripheral side wall of the groove (11) and abutting against the valve seat (321) to restrict the valve seat (321) between the valve cover (33) and a bottom wall of the groove (11); the valve cover (33) includes a first through-hole (331) and an operating portion of the valve shaft (322) that passes through the first through-hole (331); The valve stem (322) operating portion is configured to connect with an external operating member, and a valve cover (33); The microfluidic chip according to any one of configurations 1 to 5, comprising: [Configuration 7] 7. The microfluidic chip of claim 6, wherein the valve cover (33) abuts against a circumferential edge of the valve seat (321). [Configuration 8] Further provided with a sealing film, The at least two storage chambers (12) comprise a reagent chamber (121); the sealing film is configured to seal the reagent chamber (121); The microfluidic chip further comprises a top cover (4) and a puncture needle (41); The top cover (4) is disposed at one end of the storage member (1) having the groove (11), The puncture needle (41) is connected to the top cover (4), and 8. The microfluidic chip according to any one of configurations 1 to 7, wherein the puncture needle (41) is configured to press against the sealing film in order to puncture the sealing film under the action of an external force. [Configuration 9] The top cover (4) has a first rib (421), The puncture needle (41) is connected to the first rib (421), and The microfluidic chip of configuration 8, wherein the first rib (421) is configured to be cut under the action of the external force, so that the puncture needle (41) is separated from the top cover (4) and pressed against the sealing film. [Configuration 10] The central portion of the top cover (4) is provided with a second through-hole (43), and The microfluidic chip according to Aspect 8 or 9, wherein the second through-hole (43) is configured so that the external operating member can pass through to operate the valve (3). [Configuration 11] The puncture needle (41) has a needle inner gas channel (411) therein; and A microfluidic chip according to any one of configurations 8 to 10, wherein a third through-hole (412) that connects the outside of the puncture needle (41) with the needle inner gas channel (411) is positioned near the part of the puncture needle that connects with the top cover (4). [Configuration 12] Further provided with a cover sheet (6), The cover sheet (6) is disposed within the top cover (4), The cover sheet (6) has a fourth through-hole (61) through which the puncture needle (41) can pass, and The microfluidic chip of configuration 11, wherein the puncture needle (41) is configured to press the sealing film under the action of an external force, and continues to press the sealing film even after puncturing the sealing film, so that the third through hole (412) is sealed by the cover sheet (6). [Configuration 13] 13. The microfluidic chip according to any one of configurations 1 to 12, wherein the reaction chamber (21) protrudes towards one side away from the storage member (1). [Configuration 14] 14. The microfluidic chip of claim 13, wherein the reaction chamber (21) has a spherical cap structure. [Configuration 15] Further provided with an amplifying member (5), The amplification member (5) comprises an amplification chamber (51), The side of the storage member (1) is provided with a slot (14), The slot (14) is located between two adjacent storage chambers (12), and the amplifying member (5) connects with the slot (14) in an insertion manner; and 15. The microfluidic chip of any one of configurations 1 to 14, wherein the valve (3) is configured to operatively connect the reaction chamber (21) with the amplification chamber (51). [Configuration 16] The storage member (1) comprises a second storage member inner flow channel (15), a first end (151) of the second storage member inner flow channel (15) passing through the slot (14); a second end (152) of the second storage member inner flow channel (15) passing through the groove (11); The amplification member (5) comprises an amplification member inner flow channel (52) communicating with the amplification chamber (51); the amplification member inner flow channel (52) communicates with the first end (151) of the second storage member inner flow channel (15); and The microfluidic chip of configuration 15, wherein the valve (3) is operatively connected to the second end (152) of the second storage member inner flow channel (15) and is configured to direct the solution in the reaction chamber (21) through the second storage member inner flow channel (15) and the amplification member inner flow channel (52) to the amplification chamber (51). [Configuration 17] The storage member (1) comprises a third storage member inner flow channel (16); a first end (161) of the third storage member inner flow channel (16) passing through the slot (14); a second end (162) of the third storage member inner flow channel (16) passing through the groove (11); The amplifier member (5) has an amplifier member inner gas channel (53) that communicates with the amplifier chamber (51), the amplification member inner gas channel (53) communicates with the first end (161) of the third storage member inner flow channel (16); and The microfluidic chip of configuration 16, wherein the valve (3) is operatively connected to the second end (162) of the third storage member inner flow channel (16) and is configured to direct gas in the amplification chamber (51) through the amplification member inner gas channel (53) and the third storage member inner flow channel (16) to one storage chamber (12). [Configuration 18] The valve inner flow channel (31) communicating with the reaction chamber (21) is disposed within the valve (3); A valve inner gas channel (34) is also disposed within the valve (3), and A microfluidic chip according to any one of configurations 15 to 17, wherein the valve (3) is configured to operatively connect the valve inner flow channel (31) to the reaction chamber (21) and the amplification chamber (51), and to connect the valve inner gas channel (34) to the amplification chamber (51) and one storage chamber (12). [Configuration 19] The storage member (1) comprises a storage member inner gas channel (17), The storage member inner gas channel (17) communicates with the reaction chamber (21), and 19. The microfluidic chip according to any one of configurations 1 to 18, wherein the storage member inner gas channel (17) is configured to communicate with an external air pump. [Configuration 20] a first end (171) of the storage member inner gas channel (17) passing through one end of the storage member (1) comprising the groove (11); and 20. The microfluidic chip of claim 19, wherein the first end (171) of the storage member inner gas channel (17) is located between two adjacent storage chambers (12). [Configuration 21] The top cover (4) is fixedly disposed on one end of the storage member (1) having the groove (11), and 13. The microfluidic chip according to any one of configurations 8 to 12, wherein the base (2) is fixedly disposed at one end of the storage member (1) remote from the top cover (4). [Configuration 22] A microfluidic chip detection system comprising a detection device and the microfluidic chip according to any one of configurations 1 to 21, The detection device comprises an operation table for accommodating the microfluidic chip and an operation member for operating the valve (3), in a microfluidic chip detection system.

Claims

1. A microfluidic chip comprising: a storage member (1) having a groove (11) therein and at least two storage chambers (12) arranged around said groove (11); a base (2) disposed at one end of the storage member (1) remote from the groove (11), the reaction chamber (21) being disposed on the base (2); a valve (3) disposed in the groove (11), the valve (3) being configured to operatively connect any one of the at least two storage chambers (12) with the reaction chamber (21); At least two first storage member inner flow channels (13) are disposed within said storage member (1); Each first storage member inner flow channel (13) is in communication with a corresponding one of the storage chambers (12); A valve inner flow channel (31) communicating with the reaction chamber (21) is disposed in the valve (3); and the valve (3) is configured to place the valve inner flow channel (31) in operative communication with any first storage member inner flow channel (13); a first end (131) of each first storage member inner flow channel (13) penetrates the bottom wall of said groove (11); the valve (3) is configured to place the valve inner flow channel (31) in operative communication with the first end (131) of the first storage member inner flow channel (13); and A microfluidic chip, wherein a second end (132) of each first storage member inner flow channel (13) communicates with the storage chamber (12) through one side of the storage chamber (12) adjacent to the base (2).

2. 2. The microfluidic chip of claim 1, wherein the second end (132) of the first storage member inner flow channel (13) communicates with a portion of the storage chamber (12) at a lowest point.

3. a first end (311) of the valve inner flow channel (31) and a second end (312) of the valve inner flow channel (31) both pass through one end of the valve (3) adjacent to the bottom wall of the groove (11); the first end (311) of the valve inner flow channel (31) communicates with the reaction chamber (21); the second end (312) of the valve inner flow channel (31) is in operative communication with any first storage member inner flow channel (13); The first end (311) of the valve inner flow channel (31) is located in the center of the valve (3), and 3. The microfluidic chip of claim 1 or 2, wherein the second end (312) of the valve inner flow channel (31) is near the outer edge of the valve (3).

4. The valve (3) a rotor (32) rotatably disposed within the groove (11), The rotor (32) includes a valve seat (321) and a valve stem (322). a rotor (32), the valve stem (322) being connected to the valve seat (321); A valve cover (33), a valve cover (33) connected to the peripheral side wall of the groove (11) and abutting against the valve seat (321) to restrict the valve seat (321) between the valve cover (33) and a bottom wall of the groove (11); The valve cover (33) includes a first through-hole (331) and an operating portion of the valve shaft (322) that passes through the first through-hole (331), a valve cover (33) configured to connect the valve stem (322) operating portion to an external operating member; The microfluidic chip of claim 1 , comprising:

5. The microfluidic chip of claim 4 , wherein the valve cover (33) abuts against a circumferential edge of the valve seat (321).

6. Further provided with a sealing film, The at least two storage chambers (12) comprise a reagent chamber (121); the sealing film is configured to seal the reagent chamber (121); The microfluidic chip further comprises a top cover (4) and a puncture needle (41); The top cover (4) is disposed at one end of the storage member (1) having the groove (11), The puncture needle (41) is connected to the top cover (4), and The microfluidic chip according to claim 1 , wherein the puncture needle (41) is configured to press against the sealing film to puncture the sealing film under the action of an external force.

7. The top cover (4) has a first rib (421), The puncture needle (41) is connected to the first rib (421), and The microfluidic chip of claim 6, wherein the first rib (421) is configured to be cut under the action of the external force, so that the puncture needle (41) is separated from the top cover (4) and pressed against the sealing film.

8. The central portion of the top cover (4) is provided with a second through hole (43), and The microfluidic chip according to claim 6, wherein the second through-hole (43) is configured so that the external operating member can pass through to operate the valve (3).

9. The puncture needle (41) has a needle inner gas channel (411) therein; and The microfluidic chip according to any one of claims 6 to 8, wherein a third through-hole (412) that connects the outside of the puncture needle (41) with the needle inner gas channel (411) is positioned near the portion of the puncture needle that connects with the top cover (4).

10. Further provided with a cover sheet (6), The cover sheet (6) is disposed within the top cover (4), The cover sheet (6) has a fourth through-hole (61) through which the puncture needle (41) can pass, and The microfluidic chip of claim 9, wherein the puncture needle (41) is configured to press the sealing film under the action of an external force, and continues to press the sealing film even after puncturing the sealing film, so that the third through hole (412) is sealed by the cover sheet (6).

11. 2. The microfluidic chip according to claim 1, wherein the reaction chamber (21) protrudes towards one side away from the storage member (1).

12. The microfluidic chip according to claim 11, wherein the reaction chamber (21) has a spherical crown structure.

13. Further comprising an amplifying member (5), The amplification member (5) comprises an amplification chamber (51), The side of the storage member (1) is provided with a slot (14), the slot (14) is located between two adjacent storage chambers (12), the amplifying member (5) connects with the slot (14) in an insertion manner, and The microfluidic chip of claim 1 , wherein the valve (3) is configured to operatively connect the reaction chamber (21) with the amplification chamber (51).

14. The storage member (1) comprises a second storage member inner flow channel (15), a first end (151) of the second storage member inner flow channel (15) passing through the slot (14); a second end (152) of the second storage member inner flow channel (15) passing through the groove (11); The amplification member (5) comprises an amplification member inner flow channel (52) communicating with the amplification chamber (51); the amplification member inner flow channel (52) communicates with the first end (151) of the second storage member inner flow channel (15); and The microfluidic chip of claim 13, wherein the valve (3) is operatively connected to the second end (152) of the second storage member inner flow channel (15) and is configured to direct a solution in the reaction chamber (21) through the second storage member inner flow channel (15) and the amplification member inner flow channel (52) to the amplification chamber (51).

15. the storage member (1) comprises a third storage member inner flow channel (16); a first end (161) of the third storage member inner flow channel (16) passing through the slot (14); a second end (162) of the third storage member inner flow channel (16) passing through the groove (11); The amplifier element (5) comprises an amplifier element inner gas channel (53) communicating with the amplifier chamber (51); the amplification member inner gas channel (53) communicates with the first end (161) of the third storage member inner flow channel (16); and 15. The microfluidic chip of claim 14, wherein the valve (3) is operatively connected to the second end (162) of the third storage member inner flow channel (16) and is configured to direct gas in the amplification chamber (51) through the amplification member inner gas channel (53) and the third storage member inner flow channel (16) to one storage chamber (12).

16. The valve inner flow channel (31) communicating with the reaction chamber (21) is disposed within the valve (3); A valve inner gas channel (34) is also disposed within the valve (3), and The microfluidic chip of any one of claims 13 to 15, wherein the valve (3) is configured to operatively connect the valve inner flow channel (31) to the reaction chamber (21) and the amplification chamber (51), and to connect the valve inner gas channel (34) to the amplification chamber (51) and one storage chamber (12).

17. The storage member (1) comprises a storage member inner gas channel (17), The storage member inner gas channel (17) communicates with the reaction chamber (21), and The microfluidic chip according to claim 1, wherein the storage member inner gas channel (17) is configured to communicate with an external air pump.

18. a first end (171) of the storage member inner gas channel (17) passing through one end of the storage member (1) comprising the groove (11); and 18. The microfluidic chip of claim 17, wherein the first end (171) of the storage member inner gas channel (17) is located between two adjacent storage chambers (12).

19. The top cover (4) is fixedly disposed on one end of the storage member (1) having the groove (11), and 7. The microfluidic chip of claim 6, wherein the base (2) is fixedly arranged at an end of the storage member (1) remote from the top cover (4).

20. A microfluidic chip detection system comprising a detection device and the microfluidic chip according to claim 1, The detection device comprises an operation table for accommodating the microfluidic chip and an operation member for operating the valve (3).

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