Composition for recovering from muscle fatigue and composition for reducing muscle hardness

Abstract

To provide a muscle fatigue recovery composition that is highly safe and exhibits a superior effect of muscle fatigue recovery.SOLUTION: According to the present invention, a muscle fatigue recovery composition contains: cannabidiol; and a fermented product of tea leaves by koji mold or an extract thereof.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a composition for recovering from muscle fatigue and a composition for reducing muscle hardness. [Background technology]

[0002] Cannabinoids contained in hemp (Cannabis sativa) are known to have effects such as alleviating cancer-related pain and mental anxiety, and their use in the medical field is being considered. Among cannabinoids, cannabidiol (CBD) is known as a highly safe compound with no psychotropic effects and few side effects. Cannabidiol has the effect of alleviating pain and epilepsy, and is used as a medicine to treat these diseases. It also has a mild relaxing effect and skin care effects, so the development of foods and cosmetics containing cannabidiol is actively underway. Research into new functions and uses of cannabidiol is also underway. For example, Patent Document 1 discloses the use of a gelled oil-in-water emulsion containing cannabidiol as a muscle relaxant.

[0003] Generally, when a person continues to use their muscles, muscle stiffness increases and the muscles become fatigued (muscle fatigue state). This increased muscle stiffness reduces the range of motion and hinders free movement of the body. For example, professional athletes who regularly engage in rigorous training and competitions may be unable to fully demonstrate their abilities or may even be injured. Muscle fatigue can also occur not only in sports but also in standing jobs, desk work, and other situations that require repeated movements or maintaining the same posture. Therefore, not only athletes but also everyone needs to reduce increased muscle stiffness and recover more quickly from muscle fatigue. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Special Publication No. 2023-503103 Summary of the Invention [Problem to be solved by the invention]

[0005] Although Patent Document 1 above reports that cannabidiol has a muscle relaxant effect, as mentioned above, cannabidiol has various other effects in addition to its muscle relaxant effect, such as improving pain and epilepsy, and providing a relaxing effect. Therefore, if the dose of cannabidiol is excessively high in an attempt to reduce muscle stiffness and speed up recovery from muscle fatigue, there is a possibility that effects other than the intended effect will occur. Therefore, there is a need for a composition for muscle fatigue recovery that can reduce muscle stiffness and speed up recovery from muscle fatigue without using an excessively high dose of cannabidiol.

[0006] Therefore, the present invention has been made in view of the above points, and an object of the present invention is to provide a composition for recovering from muscle fatigue that is highly safe and has a more excellent effect of recovering from muscle fatigue. [Means for solving the problem]

[0007] In order to solve the above problems, the present inventors have conducted extensive research and discovered that by combining an extract of tea leaves fermented with koji mold with cannabidiol, a composition can be obtained that dramatically improves the muscle stiffness reducing effect. Based on this finding, the present invention has been completed.

[0008] To solve the above problems, the composition for recovering from muscle fatigue of the present invention contains cannabidiol and a koji mold fermentation product of tea leaves or an extract of the koji mold fermentation product of tea leaves. By combining cannabidiol with a koji mold fermentation product of tea leaves or an extract thereof, a composition for recovering from muscle fatigue can be obtained that is effective in quickly reducing increased muscle hardness and recovering from muscle fatigue. Therefore, the composition for recovering from muscle fatigue of the present invention can safely and quickly reduce muscle hardness without increasing the dosage of cannabidiol, allowing for faster recovery from muscle fatigue. Furthermore, the koji mold fermentation product of tea leaves is highly safe because it is obtained from tea leaves, which are an edible plant, and koji mold, which is used in fermented foods.

[0009] Furthermore, in the composition for recovering from muscle fatigue of the present invention, cannabidiol is preferably blended in an amount of 1 to 15% by mass of the entire composition, thereby allowing a safe and suitable cannabidiol concentration to be selected in the composition of the present invention.

[0010] Furthermore, the extract of the koji mold fermented tea leaves of the present invention is preferably an extract extracted from the koji mold fermented tea leaves using an oily component that is liquid at room temperature as an extraction solvent. By using an oily component that is liquid at room temperature as an extraction solvent, oil-soluble active ingredients that have a muscle fatigue recovery effect can be efficiently extracted from the koji mold fermented tea leaves. Furthermore, because cannabidiol is lipophilic, it can be dissolved in the oily component used as the extraction solvent, and an oil-based composition for muscle fatigue recovery can be easily obtained in which cannabidiol and the extract of the koji mold fermented tea leaves are well mixed.

[0011] The oily component is preferably at least one component selected from the group consisting of jojoba oil, vegetable oils, and ester oils. This allows the oily component to be selected as a suitable component for use in the present invention. Jojoba oil, vegetable oils, and ester oils can efficiently extract oil-soluble active ingredients that have a muscle fatigue recovery effect from the koji mold fermentation product of tea leaves, and are also highly safe.

[0012] Furthermore, the oily component is preferably jojoba oil or a medium-chain fatty acid triglyceride. This allows for the selection of an oily component that is more preferably used in the present invention. Jojoba oil and medium-chain fatty acid triglyceride can efficiently extract oil-soluble active ingredients that have a muscle fatigue recovery effect from the koji mold fermentation product of tea leaves, and also have excellent affinity to the skin and spreadability upon application, resulting in a composition for muscle fatigue recovery that is also comfortable to use.

[0013] The composition for recovering muscle fatigue of the present invention is also preferably a massage oil. This allows for the selection of a suitable dosage form for the composition of the present invention. By applying the massage oil of the present invention to a muscle area with increased muscle hardness and massaging it, the active ingredient can be penetrated, resulting in a more rapid reduction in muscle hardness and a faster recovery from muscle fatigue.

[0014] Furthermore, the composition for promoting recovery from increased muscle hardness of the present invention contains cannabidiol and a koji mold fermentation product of tea leaves or an extract of the koji mold fermentation product of tea leaves. By combining cannabidiol with a koji mold fermentation product of tea leaves or an extract thereof, a composition is obtained that reduces increased muscle hardness and promotes recovery to the state before the increase. Therefore, the composition for promoting recovery from increased muscle hardness of the present invention can safely promote the recovery of muscles with increased muscle hardness to their original state without increasing the dosage of cannabidiol. Furthermore, the koji mold fermentation product of tea leaves is highly safe because it is obtained from tea leaves, which are an edible plant, and koji mold used in fermented foods.

[0015] Furthermore, the muscle hardness-reducing composition of the present invention contains cannabidiol and a koji mold fermentation product of tea leaves or an extract of the koji mold fermentation product of tea leaves. By combining cannabidiol with a koji mold fermentation product of tea leaves or an extract thereof, a muscle hardness-reducing composition with improved effectiveness in rapidly reducing increased muscle hardness can be obtained. Therefore, the muscle hardness-reducing composition of the present invention can safely and quickly reduce muscle hardness without increasing the dosage of cannabidiol. Furthermore, the koji mold fermentation product of tea leaves is highly safe because it is obtained from tea leaves, which are an edible plant, and koji mold used in fermented foods. [Effects of the Invention]

[0016] According to the present invention, it is possible to provide a composition for recovering from muscle fatigue, a composition for promoting recovery from increased muscle hardness, and a composition for reducing muscle hardness, which have the following excellent effects. (1) It is possible to rapidly reduce increased muscle stiffness and recover from muscle fatigue without using large doses of cannabidiol safely. (2) The koji mold-fermented tea leaves or their extracts, which are combined with cannabidiol, are highly safe because they are obtained from tea leaves, which are edible plants, and koji mold, which is used in fermented foods. [Brief explanation of the drawings]

[0017] [Figure 1] 1 is a flowchart showing an outline of the steps for preparing the koji mold fermentation product of tea leaves and its extract, which are components of the composition for recovering from muscular fatigue of the present invention. [Figure 2] 1 is a graph showing the muscle hardness reducing effect of the composition for recovering from muscle fatigue according to the present invention and a comparative sample. DETAILED DESCRIPTION OF THE INVENTION

[0018] The composition for recovering from muscle fatigue, the composition for promoting recovery from increased muscle hardness, and the composition for reducing muscle hardness according to the present invention will be described below.

[0019] (Composition for recovering muscle fatigue) First, the composition for recovering from muscle fatigue according to the present invention will be described in detail. The composition for recovering from muscle fatigue according to the present invention contains cannabidiol and a product of fermentation of tea leaves with Aspergillus oryzae or an extract of the product of fermentation of tea leaves with Aspergillus oryzae.

[0020] (Cannabidiol) First, we will explain the cannabidiol contained in the composition for recovering muscle fatigue of the present invention. Cannabidiol is a type of cannabinoid extracted from hemp (Cannabis sativa). In the present invention, cannabidiol extracted and purified from plants such as hemp stems and seeds, or cannabidiol obtained by chemical synthesis can be used. When using raw materials extracted from plants, from the perspective of safety, it is preferable to use raw materials from which cannabinoids other than cannabidiol have been separated and removed. In the composition for recovering muscle fatigue of the present invention, cannabidiol is a component that at least reduces increased muscle hardness and has the effect of recovering from muscle fatigue.

[0021] From the viewpoints of safety and muscle hardness reducing effect, the amount of cannabidiol in the composition of the present invention is preferably 1 to 15% by mass of the total composition, more preferably 3 to 15% by mass, and particularly preferably 5 to 12% by mass.

[0022] (Koji mold fermented tea leaves) Next, we will explain the koji mold-fermented product of tea leaves contained in the composition for recovering muscle fatigue of the present invention. In the present invention, the koji mold-fermented product of tea leaves refers to so-called "koji" tea leaves, which are produced by seeding koji mold on tea leaves mainly picked from tea plants and allowing the koji mold to grow on the tea leaves. This koji mold-fermented product of tea leaves can be obtained, for example, by the method for producing koji mold-fermented product of tea leaves P1 shown in Figure 1. As shown in Figure 1, the method for producing koji mold-fermented product of tea leaves P1 contained in the composition of the present invention roughly consists of step S0 of preparing tea leaves as fermentation raw materials, step S1 of adding water to the tea leaves to allow them to absorb it, step S2 of steaming the tea leaves, step S3 of seeding koji mold on the steamed tea leaves, and step S4 of fermentation.

[0023] (Preparing tea leaves) First, step S0 of preparing tea leaves as the fermentation raw material shown in FIG. 1 will be described. Tea leaves from the tea plant (Camellia sinensis) can be used as the fermentation raw material in the present invention. The tea leaves may include not only tea leaves but also stems and branches harvested from the tea plant. The tea leaves used as the raw material for the koji mold-fermented product in the present invention are preferably fresh tea leaves in the state they were harvested. However, they may also be tea leaves refrigerated after harvesting, tea leaves heat-treated after harvesting, tea leaves oxidized and fermented with the oxidizing enzymes contained in the tea leaves after harvesting, or tea obtained through a tea-making process. Tea residues remaining after brewing green tea, oolong tea, black tea, or the like can also be used. Furthermore, to improve the efficiency of the subsequent steaming process and koji mold cultivation, the tea leaves or tea stems may be crushed to a certain size so that they are not too large.

[0024] (Water absorption treatment) Next, the water absorption treatment step S1 will be explained. In this step, water is added to the tea leaves or the tea leaves are soaked in water to absorb water. The amount of water absorption is not particularly limited, but specifically, the moisture content of the tea leaves is adjusted to 20 to 60%, preferably 30 to 50%.

[0025] (Steaming treatment) Next, the steaming treatment step S2 will be described. The tea leaves that have absorbed water in the above-mentioned step are placed in a steamer, the lid is closed, and the steamer is heated to expose the tea leaves to steam and heat them. The steaming treatment time is preferably about 30 to 120 minutes, more preferably about 45 to 90 minutes, and particularly preferably about 60 minutes. By performing this step, undesirable bacteria in the raw materials are sterilized, and in the subsequent fermentation step, fermentation is carried out with koji mold as the dominant species. Furthermore, when raw tea leaves are used as the fermentation raw material, this step sterilizes undesirable bacteria in the tea leaves and inactivates the oxidase contained in the tea leaves, allowing koji mold to efficiently propagate on the tea leaves in the subsequent step. In addition to a steamer, steaming treatment can also be carried out using a steaming device that can introduce steam into the device. After steaming, the tea leaves are removed from the steamer, spread evenly on a table, and cooled to about 30 to 40°C. This allows koji mold to be seeded on the tea leaves.

[0026] (Koji mold sowing) Next, we will explain step S3, in which koji mold is inoculated onto tea leaves. In this step, koji mold is inoculated onto tea leaves that have been cooled after the steaming process described above. In the present invention, koji mold refers to a microorganism primarily used in producing koji-fermented foods, specifically koji mold black, koji mold white, and koji mold yellow. Among these, koji mold black refers to a group of molds of the genus Aspergillus that form black or dark brown conidia and are used in the production of distilled alcoholic beverages such as awamori in Okinawa and sweet potato shochu in Kagoshima. Specific examples include, but are not limited to, Aspergillus ryukyuensis (Aspergillus awamori), Aspergillus ryukyuensis val kawachi (Kawachi black koji mold), Aspergillus saitoi, Aspergillus inui, Aspergillus usamii, and Aspergillus aureus. White koji mold refers to a group of Aspergillus molds that produce white-ochre conidia (a type of asexual spore) and are widely used in shochu production. They are known as white mutants of the black koji mold mentioned above. Specific examples include Aspergillus luchuensis mut. kawachii (also known as Aspergillus luchuensis var. kawachii kitahara). Yellow koji mold refers to a group of Aspergillus molds that produce yellow or yellow-green conidia and are primarily used in the production of sake, miso, soy sauce, and other products. Specific examples include, but are not limited to, Aspergillus oryzae, Aspergillus oryzae var. kawachii (Kawachi yellow koji mold), and Aspergillus sojae. In the present invention, it is preferable to use black koji mold or white koji mold from the viewpoint of improving the muscle hardness reducing effect and fatigue recovery promoting effect. Among these, black koji molds such as Aspergillus luchuensis var. kawachii and Aspergillus luchuensis, white koji molds such as Aspergillus luchuensis mut. kawachii, or a combination thereof are preferably used.Seeding of these koji molds can be carried out, for example, by mixing the koji mold with tea leaves placed in a sterilized bag and stirring. The koji mold is preferably sown as spores, and is preferably added so that there are 500,000 or more koji mold spores, and preferably 1,000,000 or more koji mold spores per 1 g (dry weight) of fermentation raw material. For example, if 1 g of seed koji contains 2 billion spores, then approximately 0.5 g (0.05 w / w%) of seed koji should be added per kg of tea leaves. After seeding the koji mold on the tea leaves, it is preferable to stir well to disperse the koji mold throughout the tea leaves.

[0027] (fermentation) Next, we will explain step S4, in which the koji mold seeded on the tea leaves is cultivated and fermented. In this step, the koji mold is propagated on the seeded tea leaves. First, the tea leaves to which the koji mold has been added are placed in a cultivation chamber maintained at around 30°C. As fermentation progresses and the temperature of the tea leaves rises over time, koji mold generally has difficulty growing above 40°C. Therefore, the temperature is lowered by ventilation, and the ventilation rate is adjusted so that the temperature of the tea leaves remains between 30°C and 42°C, preferably between 30°C and 40°C. The fermentation temperature is adjusted appropriately depending on the type of koji mold used for fermentation. Specifically, when white koji mold or black koji mold is selected as the koji mold, for example, the temperature of the tea leaves is adjusted to 35°C to 40°C for 12 to 30 hours after the start of cultivation by adjusting the room temperature of the fermentation chamber. Thereafter, the room temperature and other conditions are similarly adjusted to a slightly lower temperature of 30°C to 35°C, and the koji mold fermentation product P1 is considered complete when the koji mold has been cultured for a total of 1 to 4 days, preferably 36 to 72 hours, and more preferably 40 to 60 hours from the start of culture. On the other hand, if yellow koji mold is selected as the koji mold, for example, the temperature of the tea leaves is adjusted to 30°C to 35°C by adjusting the room temperature of the fermentation chamber or the like for 12 to 30 hours from the start of culture. Thereafter, the room temperature and other conditions are similarly adjusted to a slightly higher temperature of 35°C to 40°C, and the koji mold fermentation product P1 is considered complete when the koji mold has been cultured for a total of 1 to 4 days, preferably 36 to 72 hours, and more preferably 40 to 60 hours from the start of culture.

[0028] In addition, the manufacturing steps S1 to S4 of the koji mold fermented product P1 described above can also be performed using a machine (for example, a drum-type automatic koji making device) that can perform the washing of tea leaves, water absorption, steaming, seeding with koji mold, and fermentation (koji production) within the same device.

[0029] (Koji mold fermented product) The koji mold fermentation product P1 obtained is the so-called "koji" of tea leaves, in which koji mold has grown on tea leaves. The koji mold is contained in the koji mold fermentation product P1 as a viable organism (including spores). This koji mold fermentation product P1 is excellent at reducing increased muscle hardness and is useful for promoting recovery from muscle fatigue. The koji mold fermentation product P1 obtained by the above-described process contains a certain amount of moisture. However, the moisture can be removed by natural drying or low-temperature dehumidification drying to prevent the koji mold from dying. The koji mold fermentation product P1 from which the moisture has been removed can also be pulverized into powder or granules. By reducing the moisture content of the koji mold fermentation product P1, the activity and growth of the koji mold in the koji mold fermentation product P1 can be suppressed during storage. Furthermore, refrigerated storage enables long-term storage of the koji mold fermentation product P1 while keeping the koji mold alive. Even after the moisture has been removed, the muscle hardness-reducing effect of the koji mold fermentation product P1 can be effectively maintained. Furthermore, when the koji mold fermentation product P1 obtained as described above is incorporated into a composition for recovering from muscular fatigue, the koji mold fermentation product P1 can be sterilized by a known method. Even after sterilization, the muscle hardness-reducing effect of the koji mold fermentation product P1 can be effectively maintained.

[0030] (Extract of tea leaves fermented with koji mold) Next, the extract of koji mold-fermented tea leaves contained in the composition for recovering from muscular fatigue of the present invention will be described. As shown in Figure 1, the method for producing the extract P2 of koji mold-fermented tea leaves of the present invention generally comprises step S5 of adding an extraction solvent to the koji mold-fermented tea leaves P1 obtained by steps S1 to S4 described above to extract the extract.

[0031] (extraction) The extraction step S5 is described in detail below. In this extraction step S5, an extraction solvent is added to the koji mold fermented tea leaves P1 and an extraction process is performed to obtain an extract P2 of the koji mold fermented tea leaves. The extraction solvent is not particularly limited as long as it can extract components having a muscle hardness-reducing effect from the koji mold fermented tea leaves P1. Both lipophilic and hydrophilic solvents can be used. Examples of lipophilic solvents include oily components such as vegetable oils, ester oils, and hydrocarbon oils, as well as alcohols such as ethanol, methanol, propanol, propylene glycol, 1,3-butylene glycol, and glycerin, ethers such as diethyl ether, acetone, hexane, ethyl acetate, and dimethyl sulfoxide. Examples of hydrophilic solvents include water or aqueous solvents, and aqueous solvents include water in which other components such as alcohols and inorganic salts are dissolved or mixed. In addition to the lipophilic solvent and hydrophilic solvent described above, the extraction solvent may also contain other components such as preservatives and extraction aids, as long as the components do not interfere with the extraction of useful components from the koji mold fermentation product P1. In the present invention, from the viewpoint of efficiently extracting useful components having a muscle hardness-reducing effect from the koji mold fermentation product P1, it is preferable to use a lipophilic solvent as the extraction solvent.

[0032] Here, when obtaining the extract P2 of the Aspergillus oryzae fermented tea leaves of the present invention, it is more preferable to use an oily component that is liquid at room temperature as the extraction solvent among the above-mentioned lipophilic solvents. This allows the lipophilic cannabidiol to be dissolved in the oily component used as the extraction solvent, making it easy to obtain an oil-based composition for recovering muscle fatigue in which cannabidiol and the Aspergillus oryzae fermented tea leaves extract are well mixed. Oily components include vegetable oils, waxes, ester oils, hydrocarbon oils, higher alcohols, and silicone oils, and these can be used alone or in combination. Among these, vegetable oils include olive oil, avocado oil, camellia oil, macadamia seed oil, corn oil, rapeseed oil, sesame oil, castor oil, canola oil, linseed oil, sunflower oil, safflower oil, coconut oil, palm oil, palm kernel oil, cottonseed oil, and rice germ oil. Furthermore, waxes include jojoba oil. Examples of ester oils include medium-chain fatty acid triglycerides such as isopropyl myristate, decyl oleate, isostearyl laurate, isocetyl myristate, isostearyl myristate, octyldodecyl myristate, octyl palmitate, octyl stearate, octyldodecyl oleate, ethyl isostearate, cetyl isooctanoate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprylate, glyceryl tricaprylate, and caprylic / capric triglyceride (common name: MCT oil), glyceryl triisostearate, trimethylolpropane tri-2-ethylhexanoate, octyldodecyl neopentanoate, triethyl citrate, dioctyl succinate, diisopropyl adipate, and diethoxyethyl succinate. Examples of hydrocarbon oils include liquid paraffin (mineral oil), squalene, and squalane. Examples of higher alcohols include hexyldodecanol, octyldodecanol, and oleyl alcohol. Examples of silicone oils include methylpolysiloxane, methylphenylpolysiloxane, and cyclopentasiloxane.

[0033] Among the oily components described above, jojoba oil, ester oil, and vegetable oils are particularly suitable as the extraction solvent in the present invention, and one or more of these can be used in combination. Jojoba oil, also known as jojoba seed oil, is obtained by pressing jojoba seeds and is composed of esters primarily composed of monounsaturated fatty acids having 18 to 22 carbon atoms and monounsaturated alcohols having 20 to 24 carbon atoms. The ester oil is not particularly limited, but medium-chain triglycerides are preferably used. Medium-chain triglycerides are triesters of fatty acids having 6 to 12 carbon atoms and glycerin. One type of medium-chain triglyceride, caprylic / capric triglyceride, is primarily composed of a triester formed by dehydration condensation of the carboxyl groups of caprylic acid (C8) or capric acid (C10) with each of the three hydroxyl groups of glycerin. Jojoba oil, medium-chain fatty acid triglycerides, and vegetable oils are highly safe for the human body, have excellent affinity for the skin, and spread easily when applied, so they are suitable as extraction solvents for obtaining the extract P2 of the koji mold fermentation product of tea leaves.

[0034] The method for extracting the koji mold fermentation product P1 is not particularly limited as long as it can extract components having a muscle hardness-reducing effect from the koji mold fermentation product P1. However, in the present invention, it is preferable to add the koji mold fermentation product P1 to an extraction solvent, soak it, and extract it. When the koji mold fermentation product P1 is a dried product with a moisture content of less than 10%, the koji mold fermentation product P1 is preferably added to the extraction solvent so that the amount of the koji mold fermentation product P1 is 1% to 40% by mass, more preferably 2% to 30% by mass, and particularly preferably 5% to 20% by mass. Furthermore, the extraction conditions are not particularly limited as long as they can extract components having a muscle hardness-reducing effect from the koji mold fermentation product P1. For example, the extraction temperature is preferably at room temperature, about 10 to 30°C, and more preferably about 15 to 25°C. In this case, the extraction time is preferably about 2 hours to 5 days, more preferably about 6 hours to 3 days, and particularly preferably about 10 hours to 1.5 days. The extraction method is not limited to the above-mentioned method, and it is also possible to immerse the koji mold fermentation product P1 in an extraction solvent and extract under heating, pressure, or under heating and pressure.

[0035] After the above-mentioned extraction process, the residue is removed by centrifugation, filtration, decantation, or the like to obtain extract P2 of the koji mold fermentation product. As extract P2 of the koji mold fermentation product of the present invention, the extract itself, which is extract P2 of the koji mold fermentation product obtained in extraction step S5, or this extract after sterilization by filtration, autoclaving, or the like, can be used as a blending ingredient for the composition of the present invention. It is also possible to reduce the extraction solvent components from the obtained extract P2 to obtain a concentrated liquid, or to process it into a solid or powder form. In either case, the muscle hardness-reducing effect of extract P2 of the koji mold fermentation product can be effectively maintained.

[0036] The composition of the present invention can contain either or both of the above-mentioned koji mold fermented product P1 of tea leaves or extract P2 thereof. From the viewpoint of the muscle hardness reducing effect, the amount of koji mold fermented product P1 in the composition of the present invention is preferably 0.1% to 10% by mass, more preferably 0.5% to 5% by mass, and particularly preferably 1% to 5% by mass of the total composition. Furthermore, from the viewpoint of the muscle hardness reducing effect, the amount of koji mold fermented product extract P2 in the composition of the present invention is preferably an amount equivalent to 0.1% to 10% by mass, more preferably 0.5% to 5% by mass, and particularly preferably 1% to 5% by mass of the koji mold fermented product of tea leaves P1, when converted into the amount of koji mold fermented product of tea leaves P1 used as the raw material for extraction of this extract P2. For example, in the case of extract P2 extracted by adding koji mold fermentation product P1 of tea leaves to an extraction solvent so that the amount is 10% by mass (the amount of koji mold fermentation product P1 of tea leaves used as the extraction raw material is 10% by mass), this extract P2 is preferably incorporated at 1% by mass or more of the total composition, more preferably 5% to 50% by mass, and particularly preferably 10% to 50% by mass.

[0037] In addition to the above-mentioned ingredients, the composition for recovering muscle fatigue according to the present invention may contain appropriate ingredients commonly used in topical skin preparations, such as oily bases, flavoring agents, preservatives, stabilizers, surfactants, pigments, vitamins, excipients, powders, water, alcohols, thickeners, pH adjusters, or chelating agents, within the scope that does not impair the effects of the present invention.

[0038] The oily bases mentioned above include vegetable oils, waxes, ester oils, hydrocarbon oils, higher alcohols, higher fatty acids, and silicone oils, and can be used alone or in combination of two or more of these.These oily bases are mainly formulated for the purpose of dissolving the lipophilic component cannabidiol, so they are not particularly limited as long as they can dissolve cannabidiol.Specifically, vegetable oils include olive oil, avocado oil, camellia oil, macadamia seed oil, corn oil, rapeseed oil, sesame oil, castor oil, canola oil, linseed oil, sunflower oil, safflower oil, palm oil, coconut oil, palm oil, palm kernel oil, cottonseed oil, and rice germ oil.Furthermore, waxes include jojoba oil, beeswax, lanolin, candelilla wax, and carnauba wax. Examples of ester oils include isopropyl myristate, decyl oleate, isostearyl laurate, isocetyl myristate, isostearyl myristate, octyldodecyl myristate, octyl palmitate, octyl stearate, octyldodecyl oleate, ethyl isostearate, cetyl isooctanoate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprylate, medium-chain fatty acid triglycerides such as glyceryl tricaprylate and caprylic / capric triglyceride, glyceryl triisostearate, trimethylolpropane tri-2-ethylhexanoate, octyldodecyl neopentanoate, triethyl citrate, dioctyl succinate, diisopropyl adipate, and diethoxyethyl succinate. Examples of hydrocarbon oils include liquid paraffin (mineral oil), squalene, squalane, and petrolatum. Examples of higher alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, lanolin alcohol, hexyldodecanol, cetostearyl alcohol, octyldodecanol, oleyl alcohol, isostearyl alcohol, etc. Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, isostearic acid, linoleic acid, linolenic acid, etc.Examples of silicone oils include methylpolysiloxane, methylphenylpolysiloxane, and cyclopentasiloxane. When an oily component is used as the extraction solvent to obtain the extract P2 from the koji mold fermented tea leaves, it is preferable that the oily base incorporated into the composition of the present invention is the same as the oily component used as the extraction solvent. For example, when extract P2 from the koji mold fermented tea leaves is obtained by extraction with jojoba oil, extract P2 contains jojoba oil as a solvent, so it is more preferable to use jojoba oil as the oily base. This allows cannabidiol to be dissolved in the same components as the extraction solvent for extract P2 from the koji mold fermented tea leaves, thereby obtaining a composition in which cannabidiol and the extract from the koji mold fermented tea leaves are well mixed.

[0039] The dosage form of the composition for recovering muscle fatigue of the present invention is not particularly limited, and examples thereof include liquids such as oils and lotions, suspensions (slurries), two-layer liquids, low-viscosity liquids, emulsions, gels, ointments, balms, pastes, creams, patches, aerosols, foams, packs, and powders. Liquids can be applied in any suitable manner, such as by dripping, rolling, or spraying. The composition for recovering muscle fatigue of the present invention can be applied to any of cosmetics, quasi-drugs, and pharmaceuticals. Specific products include, but are not limited to, massage oils, lotions, gels, creams, balms, patches, and bath additives.

[0040] Furthermore, the composition for recovering from muscular fatigue of the present invention can be used in combination with one or more of various commonly used functional ingredients, such as medicinal agents selected from anti-inflammatory agents, blood circulation promoters, warming agents, antioxidants, cooling agents, skin conditioning agents, etc., within the scope of not impairing the effects of the present invention. This can further enhance the effects of the present invention.

[0041] Examples of anti-inflammatory agents include one or a combination of two or more selected from glycyrrhizinic acid or a derivative thereof, glycyrrhetinic acid or a derivative thereof, salicylic acid or a derivative thereof, aminocaproic acid, allantoin, indomethacin, bisabolol, saponin, lysozyme chloride, azulene, guaiazulene, guaiazulene sulfonate, hinokitiol, photosensitizers, tranexamic acid or a derivative thereof, zinc oxide, turmeric extract, geranium herb extract, peony extract, litchi extract, and burnet extract.

[0042] Examples of blood circulation promoters and warming agents include one or a combination of two or more selected from vanillyl butyl ether, Japanese pepper extract, ginger extract, Cnidium rhizome extract, Tangerine Pepper extract, chili pepper extract, Angelica acutiloba extract, Paeonia suffruticosa extract, nonylic acid valenylamide, nicotinic acid benzyl ester, nicotinic acid β-butoxyethyl ester, capsaicin, Cantharides tincture, ichthammol, caffeine, tannic acid, α-borneol, tocopherol nicotinate, inositol hexanicotinate, acetylcholine, cepharanthine, γ-oryzanol, and the like.

[0043] Examples of antioxidants include astaxanthin, β-carotene, γ-oryzanol, kinetin, tocopherol, dibutylhydroxytoluene, flavonoids, SOD, catalase, fullerene, phytic acid, ferulic acid, chlorogenic acid, propyl gallate, green tea extract, rosemary extract, rosehip extract, calamus extract, horsetail extract, witch hazel extract, parsley extract, loquat leaf extract, grapefruit extract, meadowsweet extract, lychee extract, mugwort extract, peach leaf extract, mango extract, moutan nut extract, pine bark extract, platinum, ubiquinone, α-lipoic acid, and the like, which can be used alone or in combination.

[0044] (Method of manufacturing a composition for recovering muscle fatigue) The composition for relieving muscle fatigue according to the present invention can be produced by a known method. Specifically, it can be produced by mixing the ingredients and dispersing or dissolving the ingredients in a base component or the like. Among these, cannabidiol is a lipophilic component, so it is preferably dissolved in an oily base and then mixed with other ingredients.

[0045] The composition for recovering from muscle fatigue according to the present invention contains, in addition to cannabidiol, the aforementioned Koji mold fermented tea leaves P1 or an extract P2 of the Koji mold fermented tea leaves, and by combining cannabidiol with the Koji mold fermented tea leaves or an extract thereof, it has the effect of quickly reducing increased muscle hardness and recovering from muscle fatigue. In this specification, recovery from muscle fatigue means reducing muscle hardness in muscles whose hardness has increased due to muscle use, thereby recovering from muscle fatigue.

[0046] The method of using the composition for recovering from muscle fatigue of the present invention is to apply, spray, or stick an appropriate amount of the composition to the area where muscle hardness has increased. A massage may be performed at the same time as the application, or the composition may be applied before or after a massage. This reduces muscle hardness in the area where muscle hardness has increased, and promotes recovery from muscle fatigue.

[0047] (Composition for promoting recovery from increased muscle hardness) Furthermore, the composition for recovering from muscle fatigue according to the present invention can be used as a composition for promoting recovery from increased muscle hardness. The composition for promoting recovery from increased muscle hardness according to the present invention contains, in addition to cannabidiol, the above-mentioned Aspergillus oryzae fermentation product of tea leaves P1 or an extract P2 of the Aspergillus oryzae fermentation product of the tea leaves, and by combining cannabidiol with the Aspergillus oryzae fermentation product of tea leaves or an extract thereof, it has the effect of quickly reducing increased muscle hardness and promoting recovery to the state before the increase. In this specification, promoting recovery from increased muscle hardness means reducing muscle hardness in muscles whose hardness has increased due to muscle use and promoting recovery to the state before the increase.

[0048] (Composition for reducing muscle hardness) Furthermore, the composition for recovering muscle fatigue according to the present invention can be used as a composition for reducing muscle hardness. The composition for reducing muscle hardness according to the present invention contains, in addition to cannabidiol, the above-mentioned Aspergillus oryzae fermentation product of tea leaves P1 or an extract P2 of the Aspergillus oryzae fermentation product of the tea leaves, and by combining cannabidiol with the Aspergillus oryzae fermentation product of tea leaves or an extract thereof, it has the effect of quickly reducing increased muscle hardness. In this specification, reducing muscle hardness means reducing the muscle hardness of muscles whose hardness has increased due to muscle use. [Example]

[0049] Next, the present invention will be described in more detail with reference to examples and comparative examples, but the present invention is not limited to these examples and comparative examples.

[0050] [Example 1] 1. Preparation of Koji-fermented tea leaves In this example, fresh tea leaves harvested from tea plants were used as the fermentation raw material. Water was added to the tea leaves to bring their moisture content to approximately 50%, and they were then subjected to a water absorption process. They were then placed in a pressure steamer and steamed. The steaming process was performed twice, at 115°C for 60 minutes, with one cooling step in between. After the tea leaves were cooled to 30°C, black koji mold (Aspergillus luchuensis var. kawachii) seed koji (product name: Kawachi Genichiro Shoten Kawachikin Black Koji, 2 billion spores per 1 g of seed koji) was added to the tea leaves at a concentration of 0.1% by mass relative to the weight of the fermentation raw material tea leaves, and the mixture was allowed to ferment for 3 days. The koji-making temperature was adjusted so that the temperature of the tea leaves was 35°C to 40°C for 24 hours after the addition of the seed koji, and then adjusted slightly lower for the next 48 hours to 30°C to 35°C. The koji-fermented tea leaves obtained after three days of koji-making were then subjected to a low-temperature drying process to a moisture content of 8%, yielding a koji-fermented tea leaf product (hereinafter also referred to as "tea koji"). This koji-fermented tea leaf product was pulverized in a mill to produce a powder.

[0051] [Example 2] 2-1. Preparation of jojoba oil extract from tea leaves fermented with koji mold In this example, an extract was prepared using the koji mold fermentation product of tea leaves obtained in Example 1 above. In this example, jojoba oil (product name: Deodorized Jojoba Oil M, 99.985% jojoba seed oil, 0.015% tocopherol, product of Watahan Trading Co., Ltd.) was used as the extraction solvent. The powdered koji mold fermentation product of tea leaves obtained in Example 1 was added to the jojoba oil, mixed, and suspended. The amount of koji mold fermentation product added was 10% by mass of the total suspension (mixture of koji mold fermentation product and jojoba oil). This suspension was left to stand at room temperature (approximately 20°C) for approximately one day to perform the extraction process. After one day had passed, the suspension of koji mold fermentation product of tea leaves and jojoba oil was passed through a sterilizing filter, and the filtrate was collected to obtain an extract of the koji mold fermentation product of tea leaves (hereinafter also referred to as "tea koji extract 1").

[0052] 2-2. Preparation of medium-chain fatty acid triglyceride extract from tea leaves fermented with koji mold In this example, medium-chain fatty acid triglycerides (product name: FineNeo-MCT, main component: caprylic / capric triglyceride, product of Nippon Fine Chemical Co., Ltd.) were used as the extraction solvent. The powdered tea leaf koji fermentation product obtained in Example 1 was added to this medium-chain fatty acid triglycerides, mixed, and suspended. The amount of koji fermentation product added was 10% by mass of the entire suspension (mixture of koji fermentation product and medium-chain fatty acid triglycerides). This suspension was allowed to stand at room temperature (approximately 20°C) for approximately one day to perform the extraction process. After one day had passed, the suspension of koji fermentation product of tea leaves and medium-chain fatty acid triglycerides was passed through a sterilizing filter, and the filtrate was collected to obtain an extract of the tea leaf koji fermentation product (hereinafter also referred to as "tea koji extract 2").

[0053] [Example 3] 3-1. Preparation of Composition 1 for Recovering from Muscle Fatigue In this example, a massage oil type composition 1 for recovering from muscle fatigue was prepared using the tea koji extract 1 obtained in Example 2-1 described above, with the following formulation. ·Tea koji extract 1 20g Cannabidiol 10g (Product name: CBD isolate, purity 99% or higher, Astrasana product) 67g of jojoba oil (the same raw material as the jojoba oil used in Example 2-1) ·Fragrance 3g In preparing the solution, cannabidiol was added to jojoba oil and dissolved, and the solution was mixed with tea koji extract 1, and finally the flavoring was added and mixed to prepare the solution.

[0054] 3-2. Preparation of Composition 2 for Recovering from Muscle Fatigue In this example, a massage oil type composition 2 for recovering from muscle fatigue was prepared using the tea koji extract 2 obtained in Example 2-2 described above, with the following formulation. ·Tea koji extract 2 20g Cannabidiol 10g (Product name: CBD isolate, purity 99% or higher, Astrasana product) 70g of medium-chain triglyceride (the same raw material as the medium-chain triglyceride used in Example 2-2) For preparation, cannabidiol was added to medium-chain fatty acid triglyceride and dissolved, and the resulting solution was mixed with tea koji extract 2 to prepare the solution.

[0055] [Comparative Example 1] 1-1. Preparation of Comparative Sample 1 In this comparative example, comparative sample 1 was prepared in the same manner as in Example 3-1 with the following composition, except that tea koji extract 1 was not added and jojoba oil was used instead of tea koji extract 1. 10g of cannabidiol 87g of jojoba oil (the same raw material as the jojoba oil used in Example 3-1) ·Fragrance 3g

[0056] 1-2. Preparation of Comparative Sample 2 In this comparative example, comparative sample 2 was prepared in the same manner as in Example 3-2 with the following composition, except that tea koji extract 2 was not added and medium-chain fatty acid triglyceride was used instead of tea koji extract 2. 10g of cannabidiol 90g of medium-chain triglycerides (using the same raw materials as the medium-chain triglycerides used in Example 3-2)

[0057] [Example 4] 4. Examination of the effects of compositions for muscle fatigue recovery In order to evaluate the effects of the compositions 1 and 2 for recovering from muscle fatigue prepared in Example 3, the following test was carried out using athletes as subjects. (1) Measurement of muscle hardness increase due to training Five subjects, one athlete each in their 20s, 30s, 40s, 50s, and 60s, participated in the following training program. First, participants warmed up by riding a 10-kilometer race bicycle at 45-50 km / h. This was followed by three sprints (200-600 m x 3 sprints, speed: 75-90 km / h). This was followed by a 20-minute warm-up (20 minutes of cycling) on ​​an indoor training bike (Wattbike, manufacturer: Wattbike Ltd., Wattbike Pro Warm-up A). ​​After this, participants performed two sets of 15 bench presses using a 24-30 kg weight, one set of 10 bench presses using a 40-50 kg weight, one set of 10 bench presses using a 56-70 kg weight, and one set of three bench presses using a 72-90 kg weight.

[0058] Immediately after the above training, each subject's right and left shoulder muscles were measured for muscle hardness using a muscle hardness meter (product name: NEUTON TDM-Z2 (BT), manufactured by Tryall Co., Ltd.). Muscle hardness measurements were performed five times at the same measurement site, and the average value was calculated as muscle hardness (Pa). The following muscle hardness measurements were performed at the same measurement site using the same method.

[0059] (2) Examination of the effects of muscle fatigue recovery compositions and comparative samples For each subject in their 20s, 30s, and 40s, Muscle Fatigue Recovery Composition 1 was applied to the right shoulder, and Comparative Sample 1 was applied to the left shoulder. For each subject in their 50s and 60s, Muscle Fatigue Recovery Composition 2 was applied to the right shoulder, and Comparative Sample 2 was applied to the left shoulder. The application amount was 0.5 mL for both Muscle Fatigue Recovery Compositions 1 and 2 and Comparative Samples 1 and 2. First, the muscle hardness of each subject's right and left shoulders was measured immediately after application. Then, each subject received a 10-minute hand massage on both shoulders. Immediately after this massage, the muscle hardness of each subject's right and left shoulders was measured. Then, each subject was asked to spend two hours freely without exercising, after which the muscle hardness of each subject's right and left shoulders was measured. The results are shown in Table 1 and Figure 2 below. Table 1 also shows the reduction rate (%) of muscle hardness immediately after training.

[0060] [Table 1]

[0061] As shown in Table 1 and Figure 2, application of the muscle fatigue recovery composition of the present invention to areas where muscle hardness had increased due to exercise was found to rapidly reduce muscle hardness. More specifically, as shown in Table 1, for the left shoulder to which Comparative Samples 1 and 2 were applied, the reduction in muscle hardness from immediately after training was approximately 4% on average immediately after application, 12-14% on average immediately after massage, and only 11-13% after two hours. In contrast, for the right shoulder to which Compositions 1 and 2 for muscle fatigue recovery of the present invention were applied, the reduction in muscle hardness from immediately after training was high, 11-14% on average immediately after application, 25% on average immediately after massage, and 30% on average after two hours. Therefore, it was revealed that the muscle fatigue recovery composition of the present invention has the effect of reducing muscle hardness increased by muscle use, thereby providing an effect of recovery from muscle fatigue.

[0062] The present invention is not limited to the above-described embodiments or examples, and various modified design forms are also included within the technical scope as long as they do not deviate from the gist of the invention described in the claims. [Industrial Applicability]

[0063] The composition for recovering from muscle fatigue according to the present invention can reduce muscle hardness and recover from muscle fatigue, and can be effectively used in the fields of health care, sports, medicine, etc.

Claims

1. A composition for application to the skin to recover from muscle fatigue, comprising cannabidiol and a koji mold fermented product of tea leaves or an extract of the koji mold fermented product of tea leaves.

2. The composition for recovering muscle fatigue for application to the skin according to claim 1, characterized in that the cannabidiol is blended in an amount of 1 to 15% by mass of the total composition.

3. The composition for recovering muscle fatigue for application to the skin, as described in claim 1, characterized in that the extract of the koji mold fermented product of tea leaves is an extract extracted from the koji mold fermented product of tea leaves using an oily component that is liquid at room temperature as an extraction solvent.

4. 4. The composition for relieving muscle fatigue for application to the skin according to claim 3, wherein the oily component is at least one component selected from the group consisting of jojoba oil, vegetable oils, and ester oils.

5. 4. The composition for relieving muscle fatigue to be applied to the skin according to claim 3, wherein the oily component is jojoba oil or a medium-chain fatty acid triglyceride.

6. The composition for relieving muscle fatigue for application to the skin according to any one of claims 1 to 5, characterized in that it is a massage oil.

7. A composition for application to the skin for promoting recovery from increased muscle hardness, comprising cannabidiol and a koji mold fermentation product of tea leaves or an extract of the koji mold fermentation product of tea leaves.

8. A composition for applying to the skin to reduce muscle hardness, comprising cannabidiol and a koji mold fermentation product of tea leaves or an extract of the koji mold fermentation product of tea leaves.

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