Bagworm egg collector, and bagworm mating and egg collection methods using the same

Abstract

To develop and provide a simple and efficient breeding method of bagworm moths and a device for performing the method, and to develop and provide a method for easily collecting eggs from bagworm moths.SOLUTION: Provided are a bagworm moth egg collection tool that comprises a tubular container, and a bagworm moth breeding method and a bagworm moth egg collection method each using the egg collection tool.SELECTED DRAWING: Figure 2

Description

[Technical Field]

[0001] The present invention relates to a bagworm moth egg collection device and a simple and efficient bagworm moth mating method using the same. and egg collection methods. [Background technology]

[0002] Bagworms, the larvae of moths belonging to the Psychidae family, spin silk to They hide in spindle-shaped or cylindrical nests (bag nests) made by entangling pieces of leaves and branches, and feed. They live with the nest for the entire larval stage, sometimes moving with the nest. has been attracting attention in recent years as a highly useful new animal-derived natural fiber (Patent Document 1 and non-patent document 1).

[0003] Establishment of techniques for mass-rearing and successive breeding of bagworms in order to industrialize bagworm silk However, the bagworm silk industry is still in its infancy, and breeding techniques are still limited. It is still under development. Bagworms are collected mainly from the field, and the production of bagworm silk is This is a major obstacle to industrialization. Therefore, the artificial breeding and egg-laying technology of the bagworm moth is The development of a new method is urgently needed. Bagworms are larvae of the same order Lepidoptera, and are already used in the sericulture industry. It is possible to apply the silkworm rearing techniques established in the past, but the mating pattern and egg-laying behavior of the silkworm are unclear. The pattern is significantly different from that of the silkworm moth and cannot be applied.

[0004] In the case of silkworm moths, there are adult females (often referred to as "female moths" in this specification) and adult males. The individual moths (often referred to as "male moths" in this specification) are placed in the container after emergence so that they can be accessed. Natural mating can be induced simply by placing male and female moths in a restricted space such as a box. In addition, the hand-pairing method involves forcing the abdominal ends of male and female moths into contact with each other to establish mating. Furthermore, if you place the mated female moths on paper, The female moths do not eat afterwards, but start laying eggs on the paper, so all you need to do to collect the eggs is to collect the paper after laying. is sufficient and very easy.

[0005] On the other hand, in the case of bagworm moths, the morphology of the adult moths differs significantly between males and females in many species. Although the female moth has the general form of a moth, it is extremely unique and cannot be recognized as a moth at first glance. In particular, the Psychidae family, such as the giant silk moth and brown silk moth, In the evolutionarily new species, the female moth has lost most of its motor and sensory organs, and only has wings, It has a maggot-like morphology, lacking legs, compound eyes, antennae, and mouthparts (Fig. 1A-a).

[0006] Also, during the metamorphosis from larva to pupa, the bagworm does not make a cocoon like the silkworm, but remains in the larval stage. During the pre-pupa stage, the larvae pupate upside down in the nest with their heads facing downwards. The male moths emerge from the nest by drilling a hole at the bottom, but the female moths do not shed their puparium (Fig. 1A-b) after emerging. They are not abandoned and have completely lost the ability to move, so they spend their entire lives within the nest without going out into the outside world. .

[0007] Due to the special ecology described above, the mating and egg-laying patterns of the bagworm moth are also extremely unique. The moths stick their heads out of the opening at the bottom of the nest and release sex pheromones, which attract male moths. After reaching the nest where the female moth is hiding (Fig. 1B-c), the male inserts his abdomen into the opening at the bottom of the nest for mating. However, the female moth's copulation hole is located at the innermost part of the nest, inside the puparium (Fig. 1B-d). The male moth then extends his abdomen and inserts it between the abdomen of the female moth and the puparium in the nest, and then They find the copulatory hole at the tip of the abdomen and complete mating (Fig. 1C). After mating, the female moth enters the puparium. Eggs are laid and retained within the puparium until they hatch.

[0008] Artificial mating is also possible for the bagworm moth. However, unlike the silkworm moth, it is not a hand-pairing method. The method used is to artificially insert the abdomen of the male moth into the opening at the bottom of the nest where the female moth is hiding. This method is not only unable to grasp the condition of female moths in the nest, Since the position of the female moth's copulatory hole cannot be confirmed from outside the nest, the male moth's copulatory organ is aimed at the female moth's copulatory hole. This meant that mating efficiency was extremely low. Because it cannot be visually confirmed, pairing is cancelled even if mating has not been achieved, and fertilized eggs cannot be collected. Conversely, the forced unpairing of mating individuals can damage the female's copulatory opening. Furthermore, it was not possible to check from outside the nest whether or not the females had laid eggs, and after mating, I had to cut open the nest of the female moth and check the egg mass inside the puparium. There was also the problem that if the nest was opened, eggs could not be collected again. [Prior art documents] [Patent documents]

[0009] [Patent Document 1] WO2019 / 003364 [Non-patent literature]

[0010] [Non-Patent Document 1] Shigeyoshi Osaki, 2002, Journal of the Society of Fiber Science and Technology (Fibers and Industry), 58: 74-78 [Non-patent document 2] Sadahiro Sawada and Ryo Arakawa, 2002, Jpn.J.Ent(NS),5(4):111-119 Summary of the Invention [Problem to be solved by the invention]

[0011] The object of the present invention is to provide a simple and efficient method for mating of the bagworm moth, and an apparatus for carrying out the method. The purpose of this project is to develop and provide such equipment.

[0012] The object of the present invention is to provide a method for easily collecting eggs from bagworms and a method for preparing large amounts of first-instar bagworms. The goal is to develop and provide methods. [Means for solving the problem]

[0013] In order to solve the above problem, the present inventors first used an X-ray device to see through the inside of the puparium. This method allows us to observe the inside of the nest and the puparium from outside. However, the X-ray equipment is very complicated to carry in and set up, and it is difficult to perform fluoroscopy. It takes time and effort to get started. In addition, the running costs as well as the equipment are very expensive. The effects of X-ray irradiation on living organisms and cells cannot be ruled out. Therefore, it was not realistic to implement the project.

[0014] Next, the inventors removed the female moths from the nests and pupariums, and then Forced mating was attempted by direct contact of the abdominal end of the silkworm moth. By opening the gripping organ with tweezers or the like and grasping the end of the female moth's abdomen, mating is forcibly established. However, the male bagworm moth has a grasping organ that is shaped to grasp the female's abdomen. Therefore, the mating success rate is extremely low when using hand-pairing methods like silkworm moths. It was low.

[0015] Therefore, after removing the female moth from the nest, mating was carried out while the puparium was still in place. Mating was achieved with a very high success rate. This shows that although a nest is not necessary for mating, female moths It became clear that it is important for the puparium to be retained. Therefore, it was found that the effect of the transparent material and the multiple microscopic materials was the same as that of the natural puparium. By using a pseudopuparium made of opaque material with fine holes, the position of the female moth's copulation hole can be confirmed from the outside. Therefore, by fine-tuning the angle and direction, the male moth's copulatory organ can be accurately guided into the female moth's copulatory hole. Furthermore, it is possible to visually check whether mating has occurred, whether mating has ended, and whether eggs have been laid. This allows for observation of the mating process, dramatically improving mating success rates and egg collection efficiency. In addition, by placing a removable auxiliary member inside the artificial puparium, it is possible to easily remove the eggs from the artificial puparium. Furthermore, by providing ventilation holes in the artificial puparium, the eggs inside the artificial puparium can be kept cool and dry. In addition, the eggs obtained can be easily washed, eliminating bacteria. The present invention is based on these new findings. and provides:

[0016] (1) A bagworm egg collector consisting of a tubular container with an inner diameter that allows a female bagworm to fit inside. an insertion hole for inserting the eggs into the female adult; a storage section for storing all or part of the abdomen of the female adult; The egg collection device has an egg storage unit. (2) The egg collection device according to (1), wherein the egg holding section has a ventilation means. (3) The egg-holding unit has a detachable egg-receiving hand for storing and collecting eggs after spawning. The egg collection device according to (1) or (2), having a step. (4) The egg collection device according to any one of (1) to (3), which has a lid portion that closes the insertion hole. (5) The tubular container is made entirely or partly of a transparent material or a light-transmitting material. 10. An egg collection device according to any one of (1) to (4). (6) The egg receiving means is made of a thin film made of a transparent or light-transmitting material. , (3) The egg collection device described in. (7) An egg collection table is provided on which the egg collection device is placed so that the insertion hole faces upward. (6) An egg collection device according to any one of the above. (8) A mating method for a bagworm moth, comprising: breeding a female bagworm moth adult according to any one of (1) to (7). The abdomen of the bagworm is inserted into the insertion hole of the egg collection device from the end of the abdomen, and the abdomen is fitted into the insertion hole. A fitting step, and a step of inserting the abdominal end of a male adult Psilocybe moth between the insertion hole and the abdomen of the female adult. The mating method further comprises a mating step of introducing the eggs into the mating zone and mating. (9) The mating method described in (8), wherein the female adult individuals of the bagworm moth are wingless and legless morphological species. (10) The mating method described in (8) or (9), wherein the female adult is an unmated individual. (11) A method for collecting eggs of the bagworm moth, comprising: (1) collecting the eggs of the bagworm moth from the nest and the pupa; (6) to (7) are inserted into the insertion hole of the egg collection device for the bagworm moth from the end of the abdomen, a fitting step of fitting the female adult into the insertion hole, and a male adult of the female Psychia parvus between the insertion hole and the abdomen of the female adult. a mating step in which the abdominal end of the insect individual is inserted and mated, and a mating step in which eggs are laid in the egg-holding section of the egg-collection device. The egg collection method further comprises an egg-laying step. (12) The method for harvesting according to (11), further comprising a female removal step of removing female individuals after the spawning step. egg method. (13) A method for preparing first-instar bagworms, comprising the steps of (11) or (12) A process of incubating the eggs of the bagworm moth obtained by the egg method in the egg-keeping section, and the first-instar bagworm moths that have hatched. The preparation method includes a step of recovering first-instar bagworms. (14) A method for preparing first-instar bagworms, comprising the steps of (11) or (12) an egg recovery step of recovering the eggs of the bagworm moth obtained by the egg method from the egg-holding section; The preparation method further comprises a step of keeping the mixture warm. [Effects of the Invention]

[0017] According to the method for mating Psilocybe moths using the Psilocybe moth egg collector of the present invention, it is possible to easily and efficiently breed Psilocybe moths. This makes artificial breeding possible.

[0018] According to the method for collecting eggs of the Psilocybe moth using the egg collector of the present invention, eggs of the Psilocybe moth can be easily and large-scaled. Available in quantities.

[0019] According to the method for preparing first-instar bagworms of the present invention, first-instar bagworms can be prepared easily and in large quantities. It can be manufactured. [Brief explanation of the drawings]

[0020] [Figure 1] A: A diagram showing the morphology (a) of a female moth of the giant silk moth removed from a nest and its puparium (b). B: An image of giant silk moths mating outdoors. In the figure, c shows the nest containing the female moth, and d shows the male moth. C: A conceptual diagram showing the inside of a female moth nest of the giant silk moth during mating. Female moths (0103) of the giant silk moth and other moths hide in the puparium (0102) within the nest with their heads (0105) facing downwards towards the opening of the nest (0101), and spend their entire lives within the nest as larvae after emerging. Male moths (0104) are capable of flight, and are attracted by the sex pheromones emitted by the female moths and reach the nest where the female moths are hiding. When the male moth reaches the female moth's nest, it inserts its abdomen (0106) into the nest through the opening at the bottom, and while extending its abdomen, it finds the copulatory opening at the end of the female moth's abdomen at the very back of the puparium, inserts its copulatory organ into the opening, and mating is achieved. [Figure 2]1 is a conceptual diagram of the Psyche moth egg extractor of the present invention. A female moth and the egg masses laid are shown by dashed lines when placed in the Psyche moth egg extractor of the present invention. [Figure 3] Figures showing artificial mating of giant silkworm moths using the Psilocybe moth egg collector of the present invention. A: A diagram of a female Psilocybe moth placed in the Psilocybe moth egg collector of the present invention. This figure shows a Psilocybe moth egg collector made by improving a 1.5 mL tube. B: A diagram showing artificial mating with the abdominal end of a male moth inserted between the female moth shown in A and the Psilocybe moth egg collector. C: A diagram showing a pair of Psilocybe moths mating in the Psilocybe moth egg collector of the present invention. The arrows in the figure indicate that mating has occurred. [Figure 4] This is a diagram showing the abdominal end (0401) of a female moth of the giant silkmoth that lays eggs in the egg-holding section of the Psyche egg collector of the present invention, and the laid egg mass (0402). The egg mass consists of an average of 1000 to 3000 eggs. [Figure 5] This is a diagram showing the preparation of first-instar bagworms of the giant silk moth using the bagworm egg collector of the present invention. A: A diagram showing a large number of giant silk moth egg masses collected by the bagworm egg collection method of the present invention being kept warm in the egg holding section of the bagworm egg collector of the present invention. B: A diagram showing first-instar bagworms of the giant silk moth that have hatched after the incubation process using the method for preparing first-instar bagworms of the present invention. In addition to the large number of first-instar bagworms in the egg holding section of the bagworm egg collector, it also shows first-instar bagworms (arrows) that have escaped to the outside of the bagworm egg collector. The first-instar bagworms are then collected in the first-instar bagworm collection process. The bagworm egg collector in this figure uses a sponge plug as a ventilation means. [Figure 6] Figures showing the configuration of the egg-receiving means in the Psilocyst Psilocyst egg collector of the present invention, and a conceptual diagram of its use. Figure A shows one embodiment of the Psilocyst Psilocyst egg collector of the present invention equipped with an egg-receiving means. This figure shows a configuration in which a thin film is pushed into the entire interior of the egg collector as the egg-receiving means. Figures B to D illustrate the placement of female Psilocyst Psilocyst (B), egg-laying (C), and egg mass collection (D). Figure E shows the egg-receiving means removed from the Psilocyst Psilocyst egg collector and unfolded, showing the collected egg mass (0604). DETAILED DESCRIPTION OF THE INVENTION

[0021] 1. Bagworm egg collector 1-1. Overview The first aspect of the present invention is an egg collection device for the bagworm moth. The egg collection device of the present invention is composed of a tubular container. The egg collection device of the present invention facilitates artificial mating of the bagworm moth and improves mating efficiency. This not only enables easy and stable egg collection, but also makes it possible to

[0022] 1-2.Definition of Terms The following terms frequently used in this specification are defined below. "Bag moth" is a general term for moths belonging to the order Lepidoptera and family Psychidae. In this specification, unless otherwise specified, when referring to bagworm moths, it refers to both males and females. In this specification, the term "female adult (individual)" is often used to refer to an adult of the bagworm moth. "Male adults (individuals)" are referred to as "female moths" and "male moths," respectively.

[0023] The species of bagworm moth targeted in this specification is not limited, but the female moth must be a wingless and legless morphological species. Here, the term "wingless and legless morphotype" refers to a female moth with degenerated wings and legs. This species of bagworm moth is wingless and legless, and its sensory organs, such as compound eyes, antennae, and mouthparts, have also degenerated. The genera that include such wingless and legless species include the species with a maggot-like external form. Although not limited, species belonging to Eumeta, Mahasena, Metura, Thyridopteryx, etc. are preferred. Specifically, for example, Eumeta japonica, Eumeta minu scula), Mahasena aurea, saunders case moth (Metura elongatus), One example is the evergreen bagworm (Thyridopteryx ephemeraeformis).

[0024] "Bagus" refers to the larvae of bagworms, regardless of species, sex, or age. In this book, "first-instar bagworms" refers to bagworms in the period from hatching to the first molting. cormorant.

[0025] In this specification, "(bagworm) nest" refers to a nest built by bagworms, The nest is made of silk threads spun by the insect itself and pieces of leaves and branches woven together with the silk threads. It is a bag-like structure that can encase the entire body of the insect, and takes on a spindle-shaped, cylindrical, conical or other shape. During the larval stage, they basically hide within this nest, and in principle only emerge from the opening of the nest when feeding or moving. In addition, not only do they molt, but they also pupate inside the nest. Some female moth species live in the nest throughout their lives, including when they emerge from the nest, and mating occurs through the opening at the bottom of the nest. This is done through the department.

[0026] In this specification, the term "puparium" refers to the outer shell of the pupal stage. Unless otherwise specified, this refers to the puparium of the Psilocyst moth. The puparium is composed of cuticle and, as shown in Figure 1A-b, Even after the moth emerges from its cocoon, it retains its rigidity and maintains its original spindle shape. Both are discarded, but in some female moth species, the head and part of the thorax can be exposed even after emergence. After opening the opening, the puparium remains inside without being discarded. In the bagworm moth, the puparium serves as an egg-laying bed, and the female moth lays eggs inside the puparium.

[0027] 1-3.Configuration The structure of the Psyche moth egg collection device of the present invention is shown in Figure 2. As shown in this figure, the Psyche moth egg collection device of the present invention The container (0201) has an insertion hole (0202), a storage section (0203), and an egg-holding section (0204) as essential components. The lid (0205) or the egg-laying platform (0206) is included as an optional component. Each configuration will be explained in detail below.

[0028] 1-3-1. Bagworm egg collector (1) Structure The Psilocyst egg collector (0201) of the present invention is composed of a tubular container. "Tube-shaped container" refers to a container with an internal space that can accommodate female adults of the bagworm moth. In addition, in the Psilocybe egg collection device, at least one end of the tubular container is open, and The internal space is in contact with the outside world through the opening.

[0029] The external shape of the Psilocybe egg collector is composed of a storage section (0203), an egg-holding section (0204), and an insertion hole ( 0202) is not particularly limited as long as it is a tubular shape. For example, Parallel tube shape (e.g., cylindrical or prismatic) with almost the same surface throughout, short axis cross section A cone-shaped structure (e.g., a spindle, cone, or pyramid) with a diameter gradually decreasing toward the end Here, the "short axis cross section" refers to the length of the Psilocyst egg collector. The cross section of the egg collector of the present invention is a cross section including a short axis perpendicular to the axis of the egg collector. Except when using artificial pupa, it is not recommended to use artificial pupa that imitates the pupa of a bagworm moth. The external shape of the egg organ is preferably the same as or similar to that of a natural puparium. For example, a spindle shape (approximately The shape may be a spindle shape or a cone shape (including a roughly cone shape). In this case, a storage section (0203) may be provided at each branch end. ), an egg holding portion (0204), and an insertion hole (0202).

[0030] There is no particular limit to the size of the bagworm egg collector. The length of the long axis depends on the type of bagworm used. The length of the container (0203) and the container (0204) will be described later. The length can be determined appropriately, taking into consideration the length of the egg part (0204). Usually, it is 1.5cm or more, 2.0cm or more, or 2.5cm or more. Over, 3.0cm or more, 3.5cm or more, 4.0cm or more, or 4.5cm or more, 10cm or less, 9cm or less, 8cm or less , 7cm or less, 6.5cm or less, 6.0cm or less, 5.5cm or less, or 5.0cm or less is acceptable. The axial length, i.e. the width (outer diameter) of the bagworm egg collector, also depends on the type of bagworm used and the size of the individual. The insertion hole (0202), the storage section (0203), and the egg storage section (0204) are different depending on the components. The width of the bagworm egg collector can be determined appropriately, taking into consideration the size of the part (0204). It does not have to be uniform throughout like a cylindrical shape, but rather, like the spindle shape shown in Figure 2, it is The insertion hole may be gradually shortened from the top to the bottom. The housing portion must have an inner diameter large enough to fit the female moth abdomen. Therefore, the width of the bagworm egg collector must be at least longer than that. For example, for large species such as the giant silk moth, the ranges are 4mm to 19mm, 5mm to 18mm, and 6mm to 17mm. The range is 3mm to 1m, or 7mm to 16mm. For medium-sized species such as brown moths, the range is 3mm to 1 The thickness may be in the range of 5 mm, 4 mm to 12 mm, or 5 mm to 10 mm.

[0031] There is no limit to the thickness of the bagworm egg collector. It can be determined appropriately taking into consideration the manufacturing cost, the rigidity of the material, etc. For example, the average thickness is 0.5 mm or more, 0.6 mm or more, 0.7 mm or more, 0.8 mm or more, 0.9 mm or more, 1.0 The thickness may be 1.5 mm or more, 1.2 mm or more, or 1.5 mm or more. Preferably, the thickness is 3.0 mm or less, 2.8 mm or less, or 2. The thickness is 5 mm or less, 2.2 mm or less, or 2.0 mm or less. The thickness may be uniform throughout. The thickness may vary from part to part.

[0032] (2)Material The material of the bagworm egg collector is not particularly limited. It can be made of natural or artificial materials, or a combination of these. Here, the natural material may be, for example, the pupa shell of the bagworm itself, metal ( alloys), minerals, animal-derived materials (including bones, teeth, tusks, horns, shells, scales, and horns), plants Examples of artificial materials include synthetic materials. Resins (including thermoplastic resins, thermosetting resins, and synthetic rubber), ceramics (ceramics, enamel) Examples of synthetic resins include glass, paper, and carbon fiber. Ethylene, polypropylene, polystyrene, vinyl acetate, cellulose acetate, acrylic resin and polycarbonate.

[0033] The material of the bagworm egg collector is made entirely or partially of transparent or translucent material. This is because the position of the copulation hole of the female moth housed in the bagworm egg collection device, mating success and This is because the release of the seal and the presence or absence of spawning can be visually confirmed from the outside.

[0034] In this specification, the term "transparent material" refers to a material that has the property of very high light transmittance. This property allows you to see clearly through the material to the other side. Examples of suitable materials include glass, polyethylene, polystyrene, polycarbonate, and acrylic resin. can be.

[0035] In this specification, the term "translucent material" refers to a material that has the property of transmitting light. Although the light transmittance is lower than that of other materials, it is possible to see the condition on the other side through the material. Specific examples of the translucent material include, but are not limited to, polypropylene. .

[0036] On the other hand, even if the material is opaque or non-translucent, the container (0203) of the egg collector and / or the egg storage By providing minute holes in the part (0204), the female moths can be seen from the outside in the same way as transparent materials. At the same time, the ventilation of the storage area and egg-holding area can be improved. The diameter of the pores in such microporous materials ranges from 10 nm to 500 μm, 12 nm to 400 μm, 15 nm to 300 μm, and 18 nm to 500 μm. The diameter of the holes may be 20 nm to 200 μm, 20 nm to 150 μm, 25 nm to 120 μm, or 30 nm to 100 μm. The shape, size, rigidity and degree of internal visibility of the egg collection device's storage and egg holding parts are taken into consideration. , should be determined appropriately.

[0037] A tubular container having the above-mentioned shape, size, and material and usable as a bagworm egg collector. Examples of the tube include a 1.5 mL tube, a conical tube, and a centrifuge tube.

[0038] 1-3-2. Insertion hole The "insertion hole" (0202) is a hole for accommodating female moths in the next-mentioned accommodation section (0203) in the bagworm egg collector. After emerging, the female bagworm moth opens a hole on the head side of the puparium. The insertion hole is inserted into the artificial puparium egg collector. In this case, a hole is formed that resembles the opening.

[0039] The insertion hole has an inner diameter that fits into the abdomen of a female bagworm moth. The inner diameter is designed to be able to grasp a part of the abdomen of the female moth. The diameter of the opening of the puparium should be about the same as the abdominal width of the female moth to be housed. The "abdominal width of the moth" is the width of the cross section of the female moth abdomen that includes the minor axis perpendicular to the major axis. The width of the female moth's abdomen is not constant because it expands and contracts during mating and egg-laying, causing peristaltic movements. Therefore, the inner diameter of the insertion hole should be the average abdominal width of the female moth. The average abdominal width is calculated from the abdominal width when expanded and when contracted. Since it is sufficient that the hole can be fitted, this average width does not need to be an exact value and can be an approximate value. The average abdominal width of the female moths is preferably that just before they are placed in the bagworm egg collector. Since this varies depending on the type and individual of the bagworm moth, it should be determined appropriately according to the female bagworm moth used. For example, but not limited to, the length of the scorpion moth is 3.0mm to 15.0mm, and the length of the scorpion moth is 4.0mm to 12.0mm. , or 5.0mm to 9.0mm, and for Chaminoga, 2.0mm to 13.0mm, 2.5mm to 12.5mm, or Therefore, the inner diameter of the insertion hole of the Psia moth egg extractor of the present invention is 3.0 mm to 12.0 mm. When using a moth, the range is approximately 9.0mm ± 4.0mm, 9.0mm ± 3.0mm, or 9.0mm ± 2.0mm. , and when using a chaminoga, the dimensions are approximately 7.5mm±5.5mm, 7.5mm±4.0mm, 7.5mm±3. It is sufficient to make sure that it is within the range of 0.0mm or 7.5mm ± 2.0mm.

[0040] The shape of the insertion hole is not limited, but the cross section of the abdomen of the female moth of the present invention is preferably approximately circular or approximately elliptical. In view of this, it is preferable that the shape of the insertion hole that fits therewith is also approximately circular or approximately elliptical. I wish.

[0041] 1-3-3. Storage section The "accommodating section" (0203) is a section that can accommodate the abdomen of the female moth inserted through the insertion hole (0202). The storage section is an essential component of the bagworm egg collector (0201) and contains the female moths. In the artificial pupal shell bagworm egg collector, the female moth has an internal space for The storage section is designed to simulate the space inside the puparium where the eggs of the Psilocysts hide after emerging. It includes all openings and is connected to the egg-holding part (0204) described below.

[0042] In this specification, "to accommodate" means to accommodate all or part of the female abdomen of the bagworm moth in the internal space. It means to capture.

[0043] The shape of the container is not particularly limited. As mentioned above, the artificial puparium is designed to mimic the inside of a puparium. In view of this, for example, a cylindrical shape is preferable. The inner diameter of the storage portion is not limited, but It is preferable that the width be equal to or less than the maximum abdominal width of the female moth to be housed.

[0044] The internal space of the storage section has a shape and size that allows at least the abdomen of the stored female moth to move peristally. It is preferable to have

[0045] On the other hand, the length of the long axis of the storage section should be equal to or longer than the abdomen of the female moth to be stored. If 3 / 4 of the abdomen of the moth is to be accommodated in the accommodation unit, the length must be equal to or greater than that. When storing the part, the length must be longer than the entire length of the abdomen. The length of the abdomen varies depending on the type of bagworm moth, and should be determined based on the total length of the female moth's abdomen. For example, the total length of the female abdomen of the giant silk moth is 10.0 mm to 30.0 mm, 12.0 mm to 26.0 mm, and 14.0 mm. mm~24.0, 16.0mm~22.0mm, and for Chaminoga it is 5.0mm~26.0mm, 14.0mm ~24.0, 16.0mm~22.0.

[0046] 1-3-4. Egg storage department The egg-holding section (0204) is a section that holds the eggs after spawning. 01), which is an essential component of the storage section (0203) and is connected to and integrated with the storage section (0203). However, the two may be separable, or may be made of a different material from the housing. It's fine.

[0047] The egg-holding section is provided below the storage section, i.e., at the bottom of the Psilocyst egg-collection device, and holds the laid eggs. are kept and stored at least until the female moths have finished laying eggs or until the eggs have hatched.

[0048] The size of the egg-holding section is not particularly limited as long as it has an internal space large enough to hold eggs. The length of the internal space is, for example, 2 mm or more, 3 mm or more, 5 mm or more, 10 mm or more, or 15 mm or more, 30 m The egg-holding area should be sized to be 1.5 mm or less, 25 mm or less, or 20 mm or less. The length of the inner space may be equal to or greater than the length of the inner space.

[0049] In the case where the storage section and the egg-holding section are integrated, when the female moths are stored in the storage section, There is usually a space of several mm or more between the tail end and the bottom of the egg collector. This space is called the egg storage area. The inner space may be the same as above.

[0050] The shape of the egg-holding part is not particularly limited as long as it can hold eggs. For example, it may be a cone shape, a pyramid shape, Any vessel having a round bottom may be used.

[0051] The egg holding unit may include an egg receiving means as an optional means. This is a means for storing eggs after spawning and for collecting eggs from the bagworm egg collector. Inside the egg storage section and is configured to be detachable from the egg-holding unit.

[0052] The shape of the egg receiving means is not limited. It is a bag-like shape that conforms to the internal shape of the egg holding section or the inside of the bagworm egg collecting device. Or it may be a sheet type that is pushed into the egg storage area, or a combination of these. For example, the sheet-shaped egg receiving means can be pulled out from the top of the egg holding section or the top of the bagworm egg collecting device. It is convenient because the egg masses inside the egg-holding section can be easily collected by simply spreading it out.

[0053] The form of the egg receiving means is not particularly limited, but it is usually It may be a thin film, a fabric, a nonwoven fabric, a net, or a combination thereof. In this case, it may have one or more holes.

[0054] The material of the egg-receiving means is not limited. For example, synthetic resin (plastic, synthetic rubber), natural wood, Fat (natural rubber), cellulose (paper, plant fiber), animal fiber (hair, silk, leather, collagen) , gelatin), glass (glass fiber), and carbon fiber. Furthermore, a transparent or translucent material is preferred, which allows the presence or absence of eggs and the state inside the egg-holding area to be confirmed. Food wrap films are particularly suitable because of their excellent transparency. Materials used in the construction of the camera include, for example, polyvinylidene chloride (PVDC), polyvinyl chloride (PVC), polyethylene Examples of suitable ethylene copolymers include, but are not limited to, polypropylene (PE), and polymethylpentene (PMP).

[0055] A specific example of the structure of the egg receiving area is shown in Figure 6. In Figures A and B, the egg receiving area is made up of a thin film. The egg-receiving means (0602) is arranged throughout the inside of the bagworm egg-collection device (0601) including the egg-holding section. As shown in B and C, a female Psilocybe moth (0603) was inserted into this Psilocybe moth egg collection device. After mating with the individual, the eggs are laid in the egg-laying area. The resulting egg masses (0604) are collected after laying. After removing the female individual, pull up the egg-receiving part (0602) as shown in D, and After separation, spread the sheet-like egg receiving part (0602) as shown in E. If this is the case, the egg masses (0604) can be easily collected.

[0056] The egg holding section may optionally include ventilation means (0207). The ventilation means is an opening provided to ensure ventilation in the internal space of the egg storage section. is composed of one or more holes and / or slits that open to the outside world. In this case, the size is not limited, but the maximum width should be smaller than the diameter of the egg in order to maintain its function of holding the egg. In the case of a slit, there is no limitation on its length or width, but it is preferable that it is as small as possible. The maximum width is preferably smaller than the diameter of the egg. If a mat is provided, it should be breathable to prevent eggs from falling and first-instar bagworms from escaping. It is advisable to close the holes or slits with a material such as a filter, mesh, or sponge. The egg-holding section itself may also be made of a breathable material.

[0057] By providing ventilation means, it is possible to prevent temperature rises and excessive humidity in the egg storage area. As a result, there are undesirable effects on the development stage of eggs, such as spoilage and mold growth due to humidity. This can prevent events that have an impact.

[0058] 1-3-5. Lid part The "lid part" (0205) is an optional component of the Psilocybe egg extractor (0201), and The lid that covers the insertion hole is not necessarily required. However, after spawning, the female When the moth is removed and the insertion hole is opened, excessive drying of the eggs in the egg-holding section and the first-instar eggs are removed. The lid can prevent bagworms from escaping.

[0059] The shape, size, and material of the lid are not particularly limited as long as it can close the insertion hole. For example, it is sufficient if the width is equal to or greater than the diameter of the insertion hole and the length is equal to or less than the storage portion. The material may be natural rubber, synthetic resin, etc., but breathable materials are preferred. Porous materials (sponges), filters, plant materials (paper, etc.), balls of yarn (cotton balls, hemp balls, wool balls, The structure for closing the insertion hole is not limited. It may be a push-in type or a screw-on type such as a screw cap. The lid may be an integrated type with the P. japonica egg collection device, or may be a detachable separate type.

[0060] 1-3-6. Egg collection table The "egg collection stand" (0206) is an optional component of the bagworm egg collection device (0201), This is a stand for placing and fixing the egg collector so that the insertion hole (0202) of the egg collector faces upward.

[0061] "Above" here means that the insertion hole of the Psilocybe egg extractor is at an angle of 90 degrees to the horizontal plane. Preferably, the insertion hole is 45 to 90 degrees, 60 to 90 degrees, 70 to 90 degrees, or 80 degrees or less relative to the horizontal plane. The angle is preferably 80 to 90 degrees.

[0062] In nature, the puparium of the bagworm moth opens downwards. Since the egg mass is located at the top of the puparium, it is possible for the egg mass to fall through the opening if left as it is. Therefore, after laying eggs, the female moth covers the surface of the eggs with her own scales to harden them and They then close the opening of the puparium with their own bodies to protect the eggs, dying just before they hatch and falling out of the nest. The first-instar larvae that hatch escape to the outside world through the open puparium opening and the lower opening of the nest. do.

[0063] On the other hand, the direction of the insertion hole of the Psilocybe egg collection device of the present invention can be freely set. By placing the egg-holding part, which corresponds to the bottom of the puparium, facing upward, the egg-holding part is prevented from being bred by the scales of the female moth. A major advantage is that egg masses do not fall out of the insertion hole even if there is no surface covering or female moths present. There is.

[0064] The egg collection stand may be an integrated type with the P. mongolica egg collection device, or may be a detachable separate type. For example, one or more P. moth egg collectors are placed and held upright or inclined. Examples of suitable racks, stands, or cases include:

[0065] 2. How to breed bagworm moths 2-1. Overview The second aspect of the present invention is a method for breeding a Psyche moth. Using a bagworm moth egg collector, mating of bagworm moths is artificially established. This method solves the problems in the conventional artificial mating method of bagworm moths and allows bagworm moths to mate easily and efficiently. It can be established.

[0066] 2-2. Method The mating method for the bagworm moth of the present invention includes a mating step and a mating step as essential steps. Each step will be explained below.

[0067] 2-2-1. Fitting process The "fitting step" is a step of inserting a female bagworm moth through the insertion hole of the bagworm moth egg collector described in the first embodiment. This process allows the female moth abdomen to mate with the insertion hole (Figure 3A).

[0068] The female moths used in the method of this embodiment are individuals before oviposition. It is preferable that they are virgin individuals. The type of bagworm moth is not limited, but wingless and legless species in which the female moth has a maggot-like form are preferred. .

[0069] In this process, naked female moths separated from their nests and pupariums are used. The method of separating the female moths is not particularly limited. For example, the nest in which the female moths are hiding can be separated by hand so as not to injure the female moths. Cut it open with a knife or similar tool, break open the opening of the puparium, and then use tweezers or similar tools to pull out the female moth. In addition, after removing the pupa from the nest at the pupal stage, wait for it to emerge as an adult, and wait until the female moth emerges as an adult. After making a hole in the puparium with a needle, the female moth may be removed by the method described above.

[0070] Insert the naked female moth into the insertion hole of the bagworm egg collector and push it in until the abdomen fits into the insertion hole. Just push it in.

[0071] 2-2-2.Mating process The mating process involves artificially inserting the abdominal end of the male moth into the bagworm egg collector after the mating process to separate the male and female moths. This is a process for inducing the mating of the bagworm moths. By this process, mating of the bagworm moths is established.

[0072] In the method of this embodiment, the male moths used are of the same species as the female moths used in the mating step. It does not matter whether the individual is virgin or mated. Male moths can mate with female moths multiple times. Therefore, a male moth that has been used for artificial mating can be used again for artificial mating with another female moth.

[0073] After the fitting step, the female moth is fitted into the bagworm egg collection device, and the male moth is inserted between the fixed female moth abdomen and the insertion hole. The abdominal end of the moth is artificially inserted. There is no limit to the insertion method. For example, the male moth is held by hand and its The male moth inserts the end of its abdomen between the female moth's abdomen and the insertion hole (Figure 3B). The male then extends his abdomen to find the female moth's copulatory hole, and mating takes place (Figure 3C). If the male moth is unable to find the mating hole of the female moth, try to check the orientation and insertion position of the male moth with your hands. You can adjust it a little to make it easier for them to mate. Alternatively, if it is made of a light-transmitting material, it is convenient because it is easy to visually check the state of the inside.

[0074] 2-3.Effects The mating method for the bagworm moth of the present invention, unlike conventional methods, makes it possible to grasp the condition of the female moth. By using an egg collector made of transparent material, the male moth's copulatory organ can be accurately guided to the female moth's copulatory hole. Not only can you do this, but you can also visually check the mating process, which dramatically improves mating efficiency. It is possible to raise it.

[0075] 3. How to collect eggs of the bagworm moth Overview A third aspect of the present invention is a method for collecting eggs of the Psychia linnaea. This is a method for obtaining a large amount of eggs from the bagworm moth by using a bagworm moth egg collector. This makes it possible to easily and stably obtain large quantities of bagworm eggs.

[0076] 3-2. Method The method for collecting eggs of the bagworm moth of the present invention comprises a mating step, a copulation step, and an egg-laying step as essential steps. The method also includes a male moth removal process and a female moth removal process as optional processes. The mating step and the mating step are the same as the mating step and the mating step in the second aspect of the P. japonica mating method. That is, the method for collecting eggs of the Psilocybe spp. of this embodiment is the same as the method for mating Psilocybe spp. of the second embodiment. Therefore, here, the steps detailed in the second embodiment will be explained. The egg-laying process and male moth removal process, which are characteristic of this embodiment, will be explained below.

[0077] 3-2-1. Male moth removal process The "male moth removal process" is a process of removing male individuals after the mating process. This is a selective step in the spawning process, which is carried out, if necessary, prior to the next spawning process.

[0078] Usually, after mating, the male moth spontaneously releases the mating, returns its extended abdomen, and leaves the female moth. Therefore, after the mating process, you can leave them alone until the next spawning process. On the other hand, male moths can also be artificially separated and removed in this process. After confirming that the copulatory organ and the copulatory hole of the female moth have separated and mating has been released, the male moth will voluntarily leave. When removing the male moths, be careful not to remove the female moths from the bagworm egg collector. After pulling out the male moth abdomen while keeping it attached, you can remove it from the bagworm egg collector. The female moths can be used again for the mating process of another female moth as described above.

[0079] 3-2-2. Egg-laying process The "spawning process" is a process in which eggs are laid in the egg-holding section of the egg-collection device after the mating process. Male moth removal is an optional process that is required in the egg method and follows or follows the mating process. The temperature and humidity conditions from mating to the start of egg laying and during egg laying are not very strict. There are no particular restrictions as long as it is within the temperature range in which the bagworm moth is active. Preferably, the temperature is 20 to 25°C, and the humidity is It should be in the range of 45-70%.

[0080] If left alone without any special treatment, female moths will begin laying eggs within 24 hours after mating. The eggs are then kept in the egg-holding area as a mass. 00 eggs are laid and the eggs form into egg masses. If these egg masses are collected, it is possible to efficiently and stably It is now possible to easily obtain large quantities of bagworm eggs, which were previously difficult to obtain.

[0081] Furthermore, since the eggs can be collected in their egg form, the process of washing the eggs, which was previously difficult, becomes easier. This treatment can sterilize and disinfect the female parent moths, so if the female parent moths are infected with bacteria or viruses, In this case, it is possible to prevent vertical infection from female parent moths and horizontal infection through contact between eggs. .

[0082] Furthermore, if the material of the bagworm egg collector is transparent or translucent, the amount of eggs produced can be measured in units of number. The eggs that have been laid are not collected but are left in the egg-holding area. It is also possible to save it.

[0083] 3-2-3. Female moth removal process The "female moth removal process" is a process for removing female moths remaining in the bagworm egg collection device after the egg-laying process. This is an optional step in the method for collecting eggs from the common moth, and can be carried out after the egg-laying step if necessary.

[0084] After laying eggs, the female moth uses her scales to cover and harden the surface of the eggs to prevent them from falling off. The female moth acts as a lid on the opening of the puparium until just before hatching, keeping the egg mass inside the puparium and closing the opening. The female moths used in the bagworm egg collector of the present invention are also inserted as they are after laying eggs. It acts as a lid to close the hole, but the egg mass does not fall from the insertion hole in the bagworm egg collector, so the female moth itself No lid is required.

[0085] Therefore, in this process, the bodies of female moths that are no longer needed after egg laying are removed from the bagworm egg collector. There are no limitations on the removal method. For example, using tweezers or the like, pick up the female moth from the bagworm egg collector. After removing the female moths, a lid can be placed on the insertion hole as needed.

[0086] 3-3.Effects According to the method for collecting eggs from the bagworm moth of the present invention, it is possible to efficiently cause female moths to lay eggs after mating, Furthermore, compared to conventional methods, it is extremely easy to confirm egg laying and collect eggs.

[0087] 4. Preparation of first-instar bagworms Overview A fourth aspect of the present invention is a method for preparing first instar bagworms. Until now, there was no way to obtain bagworms in large quantities other than through wild collection, but now we have a large number of first-instar bagworms. This allows for easy and stable procurement. Substitute breeding becomes possible.

[0088] 4-2. Method The method of this embodiment is either (1) a method using a Psilocybe egg collector, or (2) a method using a Psilocybe egg collector. This is done by either collecting eggs from the spawn or by using the egg collection method.

[0089] (1) Method using a bagworm egg collector This method is obtained by the method for collecting eggs of the third aspect of the Psilocyst Moth using the apparatus for collecting eggs of the first aspect of the Psilocyst Moth. The eggs of the bagworm are kept in the egg-holding section of the bagworm egg collector until they hatch, and the hatched bagworms are kept in the first stage. This is a method of recovery. This method includes the essential steps of keeping the bagworm warm and recovering the first-instar bagworms.

[0090] (1-1) Heat retention process The "warming process" is a process of keeping the eggs of the bagworm warm. The warming temperature is set to maintain the development of the eggs. There are no particular limitations on the temperature as long as it promotes the hatching of eggs. Usually, the temperature for eggs of the bagworm moth is within the range of 15℃ to 35℃. Therefore, it is sufficient if the temperature is within this range. For example, 18°C ​​to 32°C, or The temperature should be in the range of 20℃ to 30℃.

[0091] The eggs to be incubated in this step are egg masses obtained by the method for collecting eggs of the third aspect of the Psidium spp. The eggs are kept warm in the egg-holding section of the bagworm egg collection device. The temperature in the incubator may be adjusted to the predetermined temperature.

[0092] There is no limit to the incubation period, but it is usually sufficient to keep the eggs in the incubation period from the time they are laid until they hatch. The time it takes for the eggs to hatch can vary depending on the species of the bagworm and the conditions for hatching. When kept at the predetermined temperature, the survival time of the giant silkworm moth is approximately 14 to 30 days, for example, 17 days. For example, it is 14 to 27 days. become.

[0093] By using a transparent or translucent material for the material of the bagworm egg collector, the time from egg laying to hatching can be During storage, the condition of the eggs in the egg storage area can be visually tracked, and mold growth, egg rot, drying, etc. can be monitored. In addition, the hatching of bagworms can occur several times a year. The process of hatching occurs in several stages over the course of several days, but by being able to visualize the process up to hatching, it is possible to accurately determine the start of the hatching process. It is now possible to accurately determine the start and end times, and to adjust the rearing environment after hatching accordingly. can also be arranged in advance.

[0094] (1-2) First-instar bagworm collection process The "first-instar bagworm recovery process" is a process of collecting first-instar bagworms that have hatched in the egg-holding section after the incubation process. This is a process of collecting the silkworms from the egg part. There is no particular restriction on the method of collection, but the method of collecting the silkworms from the first instar silkworms in the sericulture industry is For example, the first-instar bagworms in the egg-holding area can be swept away with a feather broom or brush. Once the bagworms are moved, they can be raised in a suitable location. After the move, the first-instar bagworms will immediately start breeding using the nesting material. This allows for the introduction of a large number of first-instar bagworms, which was difficult with conventional technology. hands become possible.

[0095] (2) How to collect eggs from a bagworm egg collector This method is obtained by the method for collecting eggs of the third aspect of the Psilocyst Moth using the apparatus for collecting eggs of the first aspect of the Psilocyst Moth. The eggs of the bagworm moth are collected from the egg-holding section of the bagworm moth egg collector and kept outside until they hatch. This is a method for collecting worms at the first stage. In this method, the egg collection process and the incubation process are essential steps. The method also includes a step of recovering first-instar bagworms as a selection step.

[0096] (2-1) Egg collection process The "egg collection step" is a step of collecting eggs obtained by the third aspect of the bagworm egg collection method from the egg storage section. In this process, the eggs after the spawning process are taken out from the insertion hole or from another opening of the bagworm egg collector. The method of collection from the egg storage area is not limited. For example, the method of sweeping can be applied. Alternatively, you can scoop out the egg masses with a small spatula like an ear pick. The eggs can also be washed out of the egg storage area and collected.

[0097] (2-2) Heat retention process The "warming step" is a step of warming the eggs of the bagworm moth. The method described in the warming process is basically the same as above, but the eggs are kept warm not inside the egg-holding section but outside the egg-collection device. This method differs from the above-mentioned (1-1) in that it is carried out outside the egg collector. Although not limited to this, it is recommended to keep the temperature in a place where the appropriate humidity is maintained to prevent excessive humidity or dryness. For example, it is desirable to transfer the collected eggs together onto a paper such as a silkworm egg-laying mat. and keeping the temperature at the temperature while moistening the base paper to prevent drying. do.

[0098] (2-3) First-instar bagworm collection process The first-instar bagworms after hatching are collected by the method described in the first-instar bagworm collection step (1-2) above. For example, use a feather broom to sweep away areas that serve as breeding grounds for bagworms. Just move it to.

[0099] In this embodiment, the eggs of the bagworm moth collected in the egg collection step are placed in a breeding container, for example. This allows for a large amount of first-instar larvae to be obtained in the rearing container without having to be collected after the incubation process. Therefore, this step is optional and may be carried out as needed. [Example]

[0100] <Example 1: Artificial mating of Psilocybe moths using a Psilocybe moth egg collector> (the purpose) Artificial mating of Psyche moths is carried out using the Psyche moth egg collection device of the present invention. (Method and Results) The final stage larvae of the field-collected giant silk moth were allowed to emerge in a natural environment, and the resulting male and female moths were Used.

[0101] The Psilocyst egg collector of the present invention contains a 1.5 mL tube (Eppendorf) with the cap cut off. The inner diameter of the insertion hole was 9.7 mm, and the combined length of the storage section and egg-holding section ( The length of the major axis of the egg collector is 30 mm.

[0102] The female moth is removed from the nest and puparium using scissors and tweezers, and inserted into the bagworm egg collector. The abdominal end was inserted through the entrance hole and fixed to the Psychia spp. egg collector (Fig. 3A).

[0103] Next, the end of the male moth's abdomen was manually inserted between the female moth's abdomen and the insertion hole (Figure 3 B).

[0104] When the male moth's abdomen is inserted between the insertion hole and the female moth's abdomen, the male moth spontaneously moves towards the female moth's copulation hole. The female moth also moves her abdomen in a peristaltic motion, and the male moth The female guided the end of her abdomen to her own mating hole, and mating was achieved within the containment area. Since it is made of a non-toxic polypropylene material, mating can be visually confirmed from the outside. This was possible (Figure 3C).

[0105] In nature, the opening of the puparium faces downward, as shown in Figure 1B and C. Moths always mate in an upside-down position. However, the results of this example, shown in Figure 3C, suggest that male moths naturally It was also revealed that mating can be achieved even in the upside-down and upside-down states. It is possible to keep the insertion hole of the bagworm egg collector facing upward, and it is easy to operate during and after mating. Management has become dramatically easier.

[0106] <Example 2: Collection of Psilocybe eggs using a Psilocybe egg collector> (the purpose) The Psyche moth egg collector of the present invention is used to collect Psyche moth eggs.

[0107] (Method and Results) After mating male and female moths of the giant silkworm moth in a silkworm moth egg collection device using the method of Example 1, The female moths were removed from the egg collector by hand. The insertion hole was kept facing upwards and the mixture was left to stand at a temperature of 24°C. Egg laying began in the egg-laying area, and about half a day later, about 2,000 eggs were obtained. This is what it looked like about three hours after the start.

[0108] Example 3: Mass production of first-instar bagworms (the purpose) A large amount of first-instar bagworms is prepared from bagworm eggs collected using the bagworm egg collector of the present invention. .

[0109] (Method and Results) The eggs obtained in Example 2 were incubated in the egg holding section at 24°C for 21 minutes in the state of egg masses as shown in FIG. 5A. This method allowed the targeted first-instar bagworms to hatch in large numbers with a high hatching rate of over 90%. We were able to achieve this (Figure 5B).

[0110] <Example 4: Psyche egg collection device equipped with egg receiving means> (the purpose) We will verify the effects of the egg-receiving means of the Psilocybe spp. egg-collection device of the present invention on egg-laying and hatching.

[0111] (method) The Psilocyst egg collector of the present invention contained a 1.5 mL tube ( A transparent thin film cut into a square of 80 mm x 80 mm was placed in this tube. Film (food packaging wrap film: Polywrap: Ube Film Co., Ltd.: Polyethylene The egg-receiving device was then pushed along the inner wall of the tube. The actual state is shown in Figure 6A. As a control, a tube without an egg receiving means was used.

[0112] As in Example 1, a female moth was inserted and mated with a male moth. After that, the presence or absence of eggs was observed from the outside. The visibility of eggs, the difficulty of egg collection, the number of fertilizations, the number of successful hatchings, and the success rate of hatching were evaluated. The calculation was carried out.

[0113] (result) The results are shown in Table 1.

[0114] [Table 1]

[0115] Eggs in which bagworm embryonic development was confirmed were considered fertilized. The hatching success rate was calculated as the ratio of the number of successfully hatched eggs to the number of fertilized eggs.

[0116] Table 1 shows that the egg-receiving device for the Psilocybe moth does not affect egg-laying. In addition, if the egg receiving means is made of a transparent material, the egg mass inside the egg holding part can be seen as long as the egg holding part is also made of a transparent material. It was also revealed that it is possible to check the presence and condition of eggs from the outside. Regarding the egg-receiving means, if there is one, pull out the egg-receiving means from the bagworm egg-collection device and On the other hand, if there is no means of receiving the eggs, It is difficult to operate the bagworm egg collector, such as turning it over and scraping out the egg masses from the insertion hole. In addition, the number of fertilizations and successful hatchings, and the hatching success rate calculated from them, were difficult to calculate. Although there is a slight decrease compared to when there is no egg method, the egg receiving method is effective for mating and eggs after spawning. It also became clear that there was little impact.

[0117] From the above results, it is clear that in egg collection using the bagworm egg collection device of the present invention, the egg masses after spawning are collected by bagworm moths. When collecting eggs from a moth egg collector, a bagworm egg collector equipped with an egg receiving means is efficient.

Claims

1. A bagworm egg collector consisting of a tubular container, an insertion hole having an inner diameter into which a female adult Psilocybe moth fits; a storage section capable of storing the abdomen of the female adult; and Egg storage area that holds eggs after spawning The egg collection device comprising:

2. 2. The egg collection device according to claim 1, wherein the egg holding section has a ventilation means.

3. The egg-holding unit has a detachable egg-receiving means therein for storing and collecting eggs after spawning. The egg collection device according to claim 1 or 2.

4. The egg collection device according to any one of claims 1 to 3, further comprising a lid portion that closes the insertion hole.

5. The tubular container is made entirely or partly of a transparent or translucent material.

5. An egg collection device according to any one of claims 4 to 4.

6. The egg receiving means is made of a thin film made of a transparent material or a light-transmitting material. Item 4. The egg collection device according to Item 3.

7. The apparatus according to any one of claims 1 to 6, further comprising an egg collection table on which the egg collection device is placed so that the insertion hole faces upward. The egg collection device according to any one of claims 1 to 4.

8. A method for breeding bagworm moths, comprising: A female adult Psilocybe moth is placed in the insertion hole of the egg collector for Psilocybe moth according to any one of claims 1 to 7. a fitting step of inserting the end of the tube from the end of the tube and fitting the abdomen of the tube into the insertion hole; The abdominal end of the male adult Psilocybe moth is inserted between the insertion hole and the abdomen of the female adult, and mating occurs. mating process The method for crossbreeding, comprising:

9. The mating method according to claim 8, wherein the female adult individuals of the bagworm moth are wingless and legless morphotypes.

10. The mating method according to claim 8 or 9, wherein the female adult individuals are virgin individuals.

11. A method for collecting eggs of a bagworm, comprising: The female adult bagworm moth separated from the nest and pupa is treated with the bagworm moth according to any one of claims 1 to 7. A fitting step of inserting the end of the abdomen into the insertion hole of the moth egg collector and fitting the abdomen into the insertion hole. 、 The abdominal end of the male adult Psilocybe moth is inserted between the insertion hole and the abdomen of the female adult, and mating occurs. mating process, and an egg-laying step in which eggs are laid in the egg-holding section of the egg-collection device; The egg collection method comprising the steps of:

12. The egg collection method according to claim 11, further comprising a female removal step of removing female individuals after the egg-laying step. 。

13. A method for preparing first instar bagworms, comprising: The method for collecting eggs of Psyche moth according to claim 11 or 12 is carried out in the egg-keeping section. A heat retention process for keeping the food warm; and First-instar bagworm collection process to collect hatched first-instar bagworms The preparation method comprising:

14. A method for preparing first instar bagworms, comprising: The method for collecting eggs of Psyche moth according to claim 11 or 12 is carried out by removing the eggs of Psyche moth from the egg-keeping section. a step of collecting eggs, and Incubation process to keep collected eggs warm The preparation method comprising:

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