Identification and isolation of human corneal endothelial cells (HCECs)

Abstract

To provide identification, isolation and / or enrichment of human corneal endothelial cells (HCECs).SOLUTION: A method comprises a positive selection process in which a cell population containing human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs in the cell population (e.g., corneal keratocytes); and / or a negative selection process in which a cell population containing HCECs is contacted with a negative affinity reagent that selectively binds to cells other than HCECs in the cell population relative to HCECs.SELECTED DRAWING: None

Description

[Technical Field]

[0001] Related Application Description This application claims the benefit of U.S. Application No. 61 / 819,146, entitled "Method for Identifying and Isolating Human Corneal Endothelial Cells (HCECs)," filed May 3, 2013, the entire disclosure of which is incorporated herein by reference. [Background technology]

[0002] Background of the Invention When the cornea's innermost layer, the endothelium, is damaged, for example, by trauma (e.g., cataract surgery), disease, or dystrophy, the cornea becomes filled with fluid (edema) and loses its optical clarity. Patients suffer from vision loss and pain as a result. Patients' only options for treating advanced disease are corneal transplant surgery (also known as penetrating keratoplasty, or PK) or desquamated endothelial keratoplasty (DSAEK), both of which are technically challenging procedures, highly invasive for patients, and have significant limitations, such as the number of available donor corneas.

[0003] Recent studies have proposed the use of human corneal endothelial cells (HCECs) obtained from cadaveric donors to replace damaged cells. See, for example, Joyce and Zhu, Cornea. 2004 Nov;23(8 Suppl):S8-S19; Engelmann, et al., Exper. Eye Res., vol. 78, no. 3, pp. 573-578, 2004. A potential advantage of such an approach is that it may allow HCECs to be expanded ex vivo before transplantation into patients, thereby overcoming the limited availability of tissue. HCECs can be grown in specialized tissue culture media for at least five passages, significantly expanding the number of cells from a single donor.

[0004] One major problem with this technology is that current identification criteria are limited to cell morphology and expression of functional genes, such as ATP1A1 (see, e.g., Kaye and Tice, Invest Ophthalmol. 1966; 522-32; Leuenberger and Novikoff, J Cell Biol. 1974; 60721-731; McCartney et al., Curr Eye Res. 1987; 61479-1486) or the tight junction marker zonula occludens-1 (ZO-1) (see, e.g., Petroll et al., Curr Eye Res. 1999 Jan;18(1):10-9), neither of which are specific for HCECs. The lack of defined surface markers specific for HCECs makes it difficult to confirm the identity of HCECs after several passages, to distinguish HCECs from contaminating cells, or to identify the subset of HCECs from the total population that is most likely to have clinical benefit. It is also difficult to separate HCECs from contaminating fibroblasts in culture, from neighboring cells in whole corneas, or from residual corneas from DSAEK.

[0005] In this regard, current isolation methods for obtaining HCECs from intact corneas involve a stripping step, in which the endothelium and its basement membrane (Descemet's membrane) are stripped from the stroma and collected. See, e.g., Ko-Hua Chen et al., "Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study," Cornea 20(7): 731-737, 2001. Thus, the collected tissue contains HCECs but may also contain keratocytes (differentiated fibroblasts that remain in the stroma). Corneal keratocytes (also referred to herein simply as "keratocytes") are undesirable contaminants in HCEC cultures because they can grow faster than HCECs and occupy the culture dish, thereby rendering the final product essentially useless. In addition to residual stromal tissue, keratocytes can also arise from human endothelial cells and naturally convert into other cell types, such as keratocytes (see, for example, G SL Peh et al., “Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation,” International Journal of Biomaterials, Volume 2012 (2012), Article ID 601302, page 8). [Prior art documents] [Patent documents]

[0006] [Patent Document 1] U.S. Application No. 61 / 819,146 [Non-patent literature]

[0007] [Non-Patent Document 1] Joyce and Zhu, Cornea. 2004 Nov;23(8 Suppl):S8-S19; Engelmann, et al., Exper. Eye Res., vol. 78, no. 3, pp. 573-578, 2004 [Non-patent document 2] Kaye and Tice, Invest Ophthalmol.1966; 522- 32; Leuenberger and Novikoff, J Cell Biol. 1974; 60721- 731; McCartney et al., Curr Eye Res. 1987; 61479-1486 [Non-patent document 3] Petroll et al., Curr Eye Res. 1999 Jan;18(1):10-9 [Non-patent document 4] Ko-Hua Chen et al., “Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study,” Cornea 20(7): 731-737, 2001 [Non-patent document 5] G SL Peh et al., “Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation,” International Journal of Biomaterials, Volume 2012 (2012), Article ID 601302, 8 pages Summary of the Invention

[0008] Some aspects of the present invention are directed to methods for the identification, enrichment, and / or isolation of human corneal endothelial cells (HCECs).

[0009] In some embodiments, the method includes a positive selection process in which a cell population comprising human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs. Cells to which the positive affinity reagent binds are then selected, resulting in the sorted cells being enriched for HCECs. In some embodiments, two or more different positive affinity reagents are used that bind to HCECs but not to cells other than HCECs.

[0010] As defined herein, "cells other than human corneal endothelial cells" (or "cells other than HCECs") encompass keratocytes as well as less useful HCECs (for example, HCECs that have undergone transformation into fibroblasts or mesenchymal cells).

[0011] In other embodiments, the method includes a negative selection process in which a cell population comprising human corneal cells is contacted with a negative affinity reagent that selectively binds to cells other than HCECs (e.g., keratocytes) relative to HCECs. Cells to which the negative affinity reagent binds are then removed, resulting in cells that are not removed being enriched for HCECs. In some embodiments, two or more different negative affinity reagents are used that bind to cells other than HCECs (e.g., keratocytes) but not to HCECs.

[0012] In some embodiments, the method includes both (a) a positive selection using one or more affinity reagents, and (b) a negative selection using one or more negative affinity reagents.

[0013] Another aspect of the present invention is directed to affinity reagents and kits that are useful for identifying, enriching and / or isolating HCECs.

[0014] Yet another aspect of the invention is directed to isolated and / or enriched cell populations containing HCECs. In some embodiments, such enriched cell populations may include affinity reagents for the purification of HCECs.

[0015] Further aspects of the invention are described in the paragraphs that follow.

[0016] Aspect 1 A method for forming a composition enriched in human corneal endothelial cells, the method comprising: (a) contacting a cell population containing human corneal cells with a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells; and (b) selecting cells to which the first positive affinity reagent binds.

[0017] Aspect 2 The method of embodiment 1, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to keratocytes, less available human corneal endothelial cells, or both.

[0018] Aspect 3 The method of embodiment 1, wherein the first positive affinity reagent selectively binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (1) to SEQ ID NO: (58) of Table 2.

[0019] Aspect 4 2. The method of embodiment 1, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to the protein product of gene X5 of Table 2, an antibody or aptamer that binds to the protein product of gene X15 of Table 2, or an antibody or aptamer that binds to the protein product of gene X25 of Table 2.

[0020] Aspect 5 2. The method of embodiment 1, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO: (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO: (27), SEQ ID NO: (28), SEQ ID NO: (29), SEQ ID NO: (30), or SEQ ID NO: (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO: (53), SEQ ID NO: (54), SEQ ID NO: (55), SEQ ID NO: (56), or SEQ ID NO: (57).

[0021] Aspect 6 Aspect 6. The method of any one of aspects 1 to 5, wherein the first positive affinity reagent comprises an antibody or an aptamer bound to a solid matrix.

[0022] Aspect 7 Aspect 7. The method according to any one of aspects 1 to 6, wherein the first positive affinity reagent comprises an antibody or an aptamer conjugated to a label.

[0023] Aspect 8 The method of embodiment 7, wherein the label is selected from a magnetic label, a hapten (e.g., biotin), and a fluorescent label.

[0024] Aspect 9 The method according to any one of aspects 1 to 8, further comprising: (a) contacting the cell population containing human corneal cells with a second positive affinity reagent that selectively binds to human corneal endothelial cells compared to cells other than human corneal endothelial cells; and (b) selecting cells to which the second positive affinity reagent is bound, wherein the second positive affinity reagent is different from the first positive affinity reagent.

[0025] Aspect 10 The method of embodiment 9, wherein the second positive affinity reagent selectively binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (1) to SEQ ID NO: (58) of Table 2.

[0026] Aspect 11 10. The method of embodiment 9, wherein the second positive affinity reagent comprises an antibody or aptamer that binds to the protein product of gene X5 of Table 2, an antibody or aptamer that binds to the protein product of gene X15 of Table 2, or an antibody or aptamer that binds to the protein product of gene X25 of Table 2.

[0027] Aspect 12 10. The method of claim 9, wherein the second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO: (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO: (27), SEQ ID NO: (28), SEQ ID NO: (29), SEQ ID NO: (30), or SEQ ID NO: (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO: (53), SEQ ID NO: (54), SEQ ID NO: (55), SEQ ID NO: (56), or SEQ ID NO: (57).

[0028] Aspect 13 Aspect 13. The method of any one of aspects 9 to 12, wherein the second positive affinity reagent comprises an antibody or an aptamer bound to a solid matrix or a label.

[0029] Aspect 14 The method according to any one of aspects 1 to 13, further comprising: (a) contacting the cell population containing human corneal cells with a first negative affinity reagent that selectively binds to cells other than human corneal endothelial cells compared to human corneal endothelial cells; and (b) removing cells to which the first negative affinity reagent is bound.

[0030] Aspect 15 15. The method of embodiment 14, wherein the first negative affinity reagent selectively binds to keratocytes, less available human corneal endothelial cells, or both, relative to human corneal endothelial cells.

[0031] Aspect 16 15. The method of embodiment 14, wherein the first negative affinity reagent selectively binds to a corneal protein selected from the protein products of genes Y1 to Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NOs: (59) to (96) of Table 2, or the first negative affinity reagent selectively binds to a corneal protein selected from the protein products of genes Z1 to Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NOs: (97) to (109) of Table 2.

[0032] Aspect 17 15. The method of embodiment 14, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to the protein product of gene Y6 of Table 2.

[0033] Aspect 18 15. The method of embodiment 14, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO: (66), SEQ ID NO: (67), or SEQ ID NO: (68).

[0034] Aspect 19 Aspect 19. The method of any one of aspects 14 to 18, wherein the first negative affinity reagent comprises an antibody or an aptamer bound to a solid matrix or a label.

[0035] Aspect 20 A composition enriched in human corneal endothelial cells, produced by the method according to any one of aspects 1 to 19.

[0036] Aspect 21 A kit comprising: (a) a positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells; and (b) a negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.

[0037] Aspect 22 The kit according to aspect 21, wherein the positive affinity reagent selectively binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (1) to SEQ ID NO: (58) of Table 2.

[0038] Aspect 23 22. The kit of embodiment 21, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to the protein product of gene X5 of Table 2, an antibody or aptamer that binds to the protein product of gene X15 of Table 2, or an antibody or aptamer that binds to the protein product of gene X25 of Table 2.

[0039] Aspect 24 22. The kit of embodiment 21, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO: (8); (b) an antibody or aptamer that binds to one or more of SEQ ID NO: (27), SEQ ID NO: (28), SEQ ID NO: (29), SEQ ID NO: (30), or SEQ ID NO: (31); or (c) an antibody or aptamer that binds to one or more of SEQ ID NO: (53), SEQ ID NO: (54), SEQ ID NO: (55), SEQ ID NO: (56), or SEQ ID NO: (57).

[0040] Aspect 25 A kit according to any one of aspects 21 to 24, wherein the positive affinity reagent comprises an antibody or an aptamer bound to a solid matrix or a label.

[0041] Aspect 26 26. The kit of any one of aspects 21 to 25, wherein the negative affinity reagent selectively binds to a corneal protein selected from the protein products of genes Y1 to Y23 of Table 2, and includes an antibody or aptamer that binds to one or more proteins selected from SEQ ID NOs: (59) to (96) of Table 2, or the negative affinity reagent selectively binds to a corneal protein selected from the protein products of genes Z1 to Z8 of Table 2, and includes an antibody or aptamer that binds to one or more proteins selected from SEQ ID NOs: (97) to (109) of Table 2.

[0042] Aspect 27 A kit according to any one of aspects 21 to 25, wherein the negative affinity reagent comprises an antibody or an aptamer that binds to the protein product of gene Y6 in Table 2.

[0043] Aspect 28 26. The kit according to any one of aspects 21 to 25, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO: (66), SEQ ID NO: (67), or SEQ ID NO: (68).

[0044] Aspect 29 A kit according to any one of aspects 21 to 28, wherein the negative affinity reagent comprises an antibody or an aptamer bound to a solid matrix or a label.

[0045] Aspect 30 An affinity reagent selected from: (a) a solid matrix or label bound to an antibody or aptamer that binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, which comprises an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (1) to SEQ ID NO: (58) of Table 2; and (b) an antibody or aptamer that binds to a corneal protein selected from the protein products of genes Y1 to Y23 of Table 2, which comprises an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (59) to SEQ ID NO: (96) of Table 2; and a solid matrix or label bound to the protein products of genes Z1 to Z8 of Table 2, which comprises an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (97) to SEQ ID NO: (109) of Table 2.

[0046] Aspect 31 A human corneal endothelial cell-enriched composition comprising: (a) human corneal cells; and (b) a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells.

[0047] Aspect 32 32. The composition of embodiment 31, comprising human corneal endothelial cells to which the first positive affinity reagent is bound.

[0048] Aspect 33 Aspect 33. The composition of any one of aspects 31 to 32, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to keratocytes, less available human corneal endothelial cells, or both.

[0049] Aspect 34 34. The composition of any one of aspects 31 to 33, wherein the first positive affinity reagent selectively binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (1) to SEQ ID NO: (58) of Table 2.

[0050] Aspect 35 34. The composition of any one of aspects 31 to 33, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to the protein product of gene X5 in Table 2, an antibody or aptamer that binds to the protein product of gene X15 in Table 2, or an antibody or aptamer that binds to the protein product of gene X25 in Table 2.

[0051] Aspect 36 34. The composition of any one of aspects 31 to 33, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO: (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO: (27), SEQ ID NO: (28), SEQ ID NO: (29), SEQ ID NO: (30), or SEQ ID NO: (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO: (53), SEQ ID NO: (54), SEQ ID NO: (55), SEQ ID NO: (56), or SEQ ID NO: (57).

[0052] Aspect 37 37. The composition of claim 31, wherein the first positive affinity reagent comprises an antibody or an aptamer conjugated to a label.

[0053] Aspect 38 38. The composition of embodiment 37, wherein the label is selected from a magnetic label, a hapten (e.g., biotin), and a fluorescent label.

[0054] Aspect 39 A composition according to any one of aspects 31 to 38, further comprising a second positive affinity reagent that selectively binds to human corneal endothelial cells compared to cells other than human corneal endothelial cells, wherein the second positive affinity reagent is different from the first positive affinity reagent.

[0055] Aspect 40 40. The composition of embodiment 39, comprising human corneal endothelial cells having a second positive affinity reagent bound thereto.

[0056] Aspect 41 The composition of any one of aspects 39 to 40, wherein the second positive affinity reagent selectively binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from the protein products of genes X1 to X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO: (1) to SEQ ID NO: (58) of Table 2.

[0057] Aspect 42 41. The composition of any one of aspects 39 to 40, wherein the second positive affinity reagent comprises an antibody or aptamer that binds to the protein product of gene X5 of Table 2, an antibody or aptamer that binds to the protein product of gene X15 of Table 2, or an antibody or aptamer that binds to the protein product of gene X25 of Table 2.

[0058] Aspect 43 41. The composition of any one of aspects 39 to 40, wherein the second positive affinity reagent comprises: (a) an antibody or aptamer that binds to SEQ ID NO: (8); (b) an antibody or aptamer that binds to one or more of SEQ ID NO: (27), SEQ ID NO: (28), SEQ ID NO: (29), SEQ ID NO: (30), or SEQ ID NO: (31); or (c) an antibody or aptamer that binds to one or more of SEQ ID NO: (53), SEQ ID NO: (54), SEQ ID NO: (55), SEQ ID NO: (56), or SEQ ID NO: (57).

[0059] Aspect 44 44. The composition of any one of aspects 39 to 43, wherein the second positive affinity reagent comprises a label.

[0060] Aspect 45 A composition according to any one of aspects 31 to 44, comprising a measurable amount of one or more negative affinity reagents that selectively bind to cells other than human corneal endothelial cells compared to human corneal endothelial cells.

[0061] Aspect 46 46. ​​The composition of embodiment 45, wherein the one or more negative affinity reagents selectively bind to keratocytes, less available human corneal endothelial cells, or both relative to human corneal endothelial cells.

[0062] Aspect 47 47. The composition of any one of aspects 45 to 46, wherein the one or more negative affinity reagents may comprise, for example, one or more antibodies that bind to one or more corneal proteins selected from the protein products of genes Y1 to Y23 of Table 2, which selectively bind to a corneal protein selected from the protein products of genes Y1 to Y23 of Table 2, including one or more antibodies or aptamers that bind to one or more proteins selected from SEQ ID NOs: (59) to (96) of Table 2; or the one or more negative affinity reagents may comprise, for example, one or more antibodies or aptamers that selectively bind to a corneal protein selected from the protein products of genes Z1 to Z8 of Table 2, which selectively bind to a corneal protein selected from the protein products of genes Z1 to Z8 of Table 2, including one or more antibodies or aptamers that bind to one or more proteins selected from SEQ ID NOs: (97) to (109) of Table 2.

[0063] Aspect 48 47. The composition of any one of aspects 45 to 46, wherein the one or more negative affinity reagents comprise an antibody or aptamer that binds to the protein product of gene Y6 of Table 2.

[0064] Aspect 49 47. The composition of any one of aspects 45 to 46, wherein the one or more negative affinity reagents comprise an antibody or aptamer that binds to one or more of SEQ ID NO: (66), SEQ ID NO: (67), or SEQ ID NO: (68).

[0065] Aspect 50 Aspect 50. The composition of any one of aspects 45 to 49, wherein the one or more negative affinity reagents comprise an antibody or an aptamer conjugated to a label.

[0066] These and various other aspects and embodiments, as well as various advantages of the present invention, will become readily apparent to those skilled in the art upon review of the detailed description and appended claims that follow. [Brief explanation of the drawings]

[0067] [Figure 1] 1A-1C are bright field photomicrographs of HCECs and keratocytes in culture. [Figure 2] Figure 2 presents in bar graph format the expression of four surface markers in different corneal cell populations analyzed by flow cytometry. [Figure 3] 3A-3C are dual-color fluorescence histograms of HCECs and keratocytes. [Figure 4] FIG. 4 depicts the fluorescence profiles showing the expression of four surface markers in three different HCEC populations analyzed by flow cytometry. [Figure 5] Figure 5 presents in bar graph format the expression of four surface markers in three different HCEC populations analyzed by flow cytometry. [Figure 6] Figure 6 shows dual-color fluorescence histograms for various pairs of surface markers in three different HCEC populations. [Figure 7] FIG. 7 depicts the transendothelial electrical resistance (TEER) as a function of time for cell cultures of three different HCEC populations. DETAILED DESCRIPTION OF THE INVENTION

[0068] Detailed Description A more complete understanding of the present invention can be obtained by reference to the following detailed description of numerous aspects and embodiments of the invention, which is intended to be illustrative and not limiting of the invention.

[0069] As noted above, in some embodiments, the present disclosure relates to a positive selection process in which a cell population comprising human corneal cells is contacted with one or more positive affinity reagents that selectively bind HCECs over cells other than HCECs (e.g., keratocytes), including positive affinity reagents that selectively bind HCECs that are likely to have a higher clinical benefit than the general HCEC population.

[0070] In another aspect, the present disclosure relates to a negative selection process in which a cell population comprising human corneal cells is contacted with one or more negative affinity reagents that selectively bind to cells other than HCECs (e.g., keratocytes) relative to HCECs.

[0071] These negative and positive selection methods may be used independently or in combination with each other, for example, to identify HCECs, to isolate HCECs, and / or to enrich a cell population for HCECs.

[0072] Cell populations suitable for enrichment or isolation of HCECs include cell populations obtained from intact or remnant human corneas, and may be derived, for example, from embryonic, fetal, or adult tissue. For example, intact corneas may undergo a stripping step in which the endothelium and its basement membrane (Descemet's membrane) are stripped from the stroma and collected. See Ko-Hua Chen et al., "Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study," Cornea 20(7): 731-737, 2001. In other embodiments, cell populations may be obtained from remnant corneas (e.g., ocular tissue remaining after a corneal button has been used for DSAEK).

[0073] Tissues obtained from intact and residual human corneas may be separated into individual cells by processes such as enzymatic and / or mechanical dissociation. In this step, cells are cultured for a period of time at room temperature or 37°C using a single enzyme or a combination of enzymes. These enzymes include some of the following: collagenase, papain, dispase, elastase, trypsin / EDTA, and / or DNase. The tissue is then mechanically dissociated using a conventional or glass pipette to obtain individual cells or cell clumps that can then be expanded in culture. See, for example, Li W. et al., Invest Ophthalmol Vis Sci 2007; 48: 614; Ishino Y. et al., Invest Ophthalmol Vis Sci 2004; 45: 800; Chen KH et al., Cornea 2001; 20: 731.

[0074] The medium in which the cells may be suspended may be any medium that maintains the viability of HCECs. A variety of media are commercially available and may be used, including Minimal Essential Medium (MEM), Dulbecco's Modified Eagle's Medium (DMEM), Opti-MEM®, Media 199 or M199, Dulbecco's Modified Eagle's Medium with Nutrient Mixture F-12 (DMEM / F-12), F99 Ham's F12, SHEM Ham's F12, EGM-2 Endothelial Growth Medium, which is often supplemented with serum of human or animal origin, BSA, HSA, growth factors, antioxidants, antibiotics, antimycotics, hormones, amino acids, and peptides. Specific examples of media are shown in Table 1 below.

[0075] [Table 1]

[0076] Cell cultures obtained from intact and remnant corneas contain unwanted contaminating cells arising from residual non-endothelial tissue (e.g., stroma, epithelium, etc.) that may be present in the sample. In the culture of HCECs, HCECs with lower cell transplantability compared to other HCECs with higher cell transplantability may also be considered "contaminants" in some forms.

[0077] Cell populations suitable for enrichment or isolation of HCECs also include HCEC cultures in which contaminating cells outgrow the HCECs or in which HCECs spontaneously convert to other cell types (e.g., keratocytes, etc.) As noted above, contaminating cells such as keratocytes are particularly undesirable where it is desired to expand HCEC cultures in vitro because they can outgrow the HCECs and thereby overwhelm the cell culture.

[0078] Accordingly, various aspects of the present invention relate to methods, reagents, and kits for separating HCECs from other cells, particularly keratocytes, and / or less useful HCECs. HCECs are separated from a mixture of cells by techniques that select for cells with specific characteristics.

[0079] Human corneal endothelial cells may be identified or sorted through (a) positive cell markers that are found on the surface of HCECs but not on the surface of contaminating cells that may be mixed with HCECs (e.g., positive sorting), (b) negative cell markers that are found on the surface of contaminating cells that are mixed with HCECs but not on the surface of HCECs (e.g., negative sorting), and combinations of positive and negative cell markers.

[0080] For example, when intact human corneas are used as a source of endothelial cells, positive cell markers may be selected from corneal proteins found in the endothelium (formed from HCECs) but not in other corneal tissues (i.e., stroma and / or epithelium). Conversely, negative cell markers may be selected from corneal proteins found in corneal tissues other than the endothelium (i.e., stroma and / or epithelium), but not in the corneal endothelium.

[0081] As another example, if the source of endothelial cells is endothelium and basement membrane separated from the stroma and epithelium of an intact cornea, positive cell markers may be selected from keratocyte proteins found in the endothelium but not in the stroma, while negative cell markers may be selected from keratocyte proteins found in the stroma but not in the corneal endothelium.

[0082] Corneal proteins that may be useful as cell markers in the context of the present invention include suitable proteins selected from the proteins presented in Table 2 set forth in Appendix A.

[0083] Positive cell markers include suitable corneal proteins selected from the protein products of genes X1 to X26 in Table 2 (e.g., SEQ ID NO: (1) to SEQ ID NO: (58)) that are present in the corneal endothelium but not in the stroma or epithelium.

[0084] Negative cell markers include (a) suitable corneal proteins selected from the protein products of genes Y1 to Y23 in Table 2, which are present in the stroma and epithelium but not in the endothelium (e.g., SEQ ID NO: (59) to SEQ ID NO: (96)), and (b) suitable corneal proteins selected from the protein products of genes Z1 to Z8 in Table 2, which are present in the stroma but not in the corneal endothelium (or epithelium) (e.g., SEQ ID NO: (97) to SEQ ID NO: (109)).

[0085] As described above, in some embodiments, the present disclosure relates to (a) a positive selection process in which a cell population comprising human corneal cells is contacted with one, two, three, four or more positive affinity reagents that selectively bind to HCECs relative to cells other than HCECs (e.g., keratocytes, etc.); (b) a negative selection process in which a cell population comprising human corneal cells is contacted with one, two, three, four or more negative affinity reagents that selectively bind to cells other than HCECs (e.g., keratocytes, etc.) relative to HCECs; and (c) a combination of (a) and (b).

[0086] For this purpose, affinity reagents that specifically bind to various corneal proteins are used: positive affinity reagents specifically bind to positive cell markers associated with HCECs, while negative affinity reagents specifically bind to negative cell markers associated with contaminating cells other than HCECs.

[0087] Various positive cell markers are described above and include corneal proteins that are found in the endothelium (formed from HCECs) but not in other corneal tissues (i.e., stroma and / or epithelium). Various negative cell markers are also described above and include corneal proteins that are found in corneal tissues other than the endothelium (i.e., stroma and / or epithelium) but not in the corneal endothelium.

[0088] Those skilled in the art will recognize that suitable negative and positive affinity reagents may be used in any order and / or in any combination.

[0089] Affinity reagents suitable for use in the present disclosure may include any species that selectively bind to a given surface marker, including positive affinity reagents that selectively bind to positive cell markers and negative affinity reagents that selectively bind to negative cell markers.

[0090] Affinity reagents particularly useful for the practice of the present invention are antibodies (also referred to herein as "affinity antibodies"), nucleic acid aptamers, and other modified forms of protein scaffolds. Antibodies include whole antibodies and antibody fragments such as, for example, Fab, F(ab'), light chain, or heavy chain fragments.

[0091] The affinity antibody selected for use will have a low level of non-specific interactions.

[0092] Affinity antibodies may be polyclonal or monoclonal and, if not commercially available, may be readily produced by techniques known to those skilled in the art.

[0093] For example, affinity antibodies to a given corneal protein may be obtained by immunizing a cross-species immunocompetent mammalian host (including mice, rodents, lagomorphs, sheep, pigs, cattle, etc.) with the corneal protein of interest. Immunization is carried out according to conventional techniques, and the corneal protein may be injected subcutaneously, intramuscularly, intraperitoneally, intravenously, etc., via one or more injection courses. After completion of the immunization schedule, antisera may be collected according to conventional methods to provide polyantisera specific for the corneal protein of interest. Lymphocytes may also be collected from appropriate lymphoid tissues, such as the spleen, draining lymph nodes, etc., and fused with an appropriate fusion partner, e.g., a myeloma line, to generate hybridomas secreting specific monoclonal antibodies. Screening of hybridoma clones for the desired antigen specificity is carried out according to conventional methods.

[0094] In many embodiments, the affinity antibody is conjugated to a suitable substrate, such as a label or a solid matrix. Labels include magnetic labels, such as magnetic beads or microparticles or nanoparticles, including superparamagnetic nanoparticles, which facilitate separation. Labels also include biotin, which binds with high affinity to avidin or streptavidin. Labels further include fluorescent dyes, which can be used with flow cytometry, such as fluorescence-activated cell sorting (FACS), to facilitate the separation of specific cell types. Fluorescence-activated cell sorters have various degrees of sophistication, including multiple color channels, low-angle and obtuse-angle light scattering detection channels, and impedance channels. Fluorescent dyes include phycobiliproteins, such as phycoerythrin and allophycocyanin, fluorescein and Texas Red, Cy7, and Cy5, among others. Conjugated antibodies, each with affinity for a specific corneal protein, may each be labeled with a different fluorescent dye to enable independent sorting (multicolor analysis) of associated cellular proteins.

[0095] Cell sorting may also be performed by "panning" with affinity antibodies attached to a solid matrix, such as a plate, immobilized beads, etc. For example, an affinity antibody having specificity for a particular corneal protein may be bound to the solid matrix, and corneal cells exhibiting the particular corneal protein can be captured by the immobilized antibody, while other cells remain in suspension and can be removed.

[0096] Any sorting technique may be used that is not unduly detrimental to the viability of the sorted cells. Combinations of the above techniques may also be used.

[0097] The exact method for coupling an antibody to a given substrate (e.g., label, solid matrix, etc.) is not critical to the practice of the present disclosure, and numerous alternative methods are known in the art. For example, an affinity antibody may be coupled directly or indirectly to a substrate. Direct coupling to a substrate can be achieved through the use of various chemical linking groups, as known in the art. For example, an antibody can be coupled to a substrate through side chain amino or sulfhydryl groups, as well as heterofunctional cross-linking reagents. Many heterofunctional compounds are available for connecting various components. Specific examples include 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) or 4-(N-maleimidomethyl)-cyclohexane-1-carboxylic acid N-hydroxysuccinimide ester (SMCC), which can react with reactive sulfhydryl groups on an antibody and reactive amino groups on a substrate.

[0098] Alternatively, the affinity antibody can be indirectly bound to the substrate through a hapten or a secondary antibody. For example, the antibody can be directly bound to the hapten, or a species that specifically binds to the hapten can be bound to the substrate. Suitable haptens include digoxin, digoxigenin, FITC, dinitrophenyl, nitrophenyl, avidin, streptavidin, biotin, and the like. For example, the antibody can be conjugated to one member of a high affinity binding system (e.g., biotin) and another member of a high affinity binding system (e.g., avidin) attached to the substrate. Methods for conjugating haptens to proteins are known in the art, and kits for such conjugation are commercially available. The secondary antibody can be directly or indirectly conjugated to the substrate.

[0099] During cell separation, the bound antibody may be combined with a suspension of cells and incubated for a period of time sufficient for the antibody to bind to proteins on the cells. The amount of antibody required to bind to a particular cell subset may be determined empirically by performing trial separations and analyses. The cells and antibody are incubated for a period of time sufficient for binding to occur.

[0100] The medium in which the cells are separated may be any medium that maintains the viability of the cells. A variety of media are commercially available, including those listed above.

[0101] The conjugated affinity antibodies include conjugated positive (for positive selection) affinity antibodies specific for corneal proteins that are present on human corneal endothelial cells and not present on contaminating cells such as the stroma and / or epithelium, and conjugated negative (for negative selection) affinity antibodies specific for corneal proteins that are present on contaminating cells such as the stroma and / or epithelium and not present on human corneal endothelial cells.

[0102] Once the antibody is bound to the cells, the bound cells are separated for a specific antibody preparation, for example, using fluorescently labeled antibodies and FACS separation, magnetically labeled antibodies and immunomagnetic separation, or immobilized antibodies and "panning."

[0103] The cells can be separated from the affinity antibody, as desired, using known techniques. In one embodiment, if the antibody in the immunopanning process is a positive selection antibody, the substrate with attached endothelial cells can be washed to remove unbound and released endothelial cells using a suitable technique (e.g., trypsin digestion).

[0104] Although various specific embodiments using antibodies as affinity reagents are specifically described herein, it is understood that other affinity reagents for binding to positive or negative cell markers, including nucleic acid aptamers and other modified forms of protein scaffolds, can be similarly used. Aptamers are synthetic oligonucleotides selected from a pool of random-sequence oligonucleotides that bind to various biomolecular targets with high affinity and specificity. See, for example, J. Wang and G. Li, "Aptamers against cell surface receptors: selection, modification, and application," Curr Med Chem. 2011;18(27):4107-16.

[0105] The separated cells may be collected in any suitable medium that maintains the viability of the cells.

[0106] Thus, a cell population enriched in HCECs may be achieved by this method. The HCEC population may comprise 50% or more of the cells in the cell composition, preferably 75% or more of the cells in the cell composition, more preferably 90% or more of the cells in the cell composition, and may represent as much as 95% or more of the cells in the cell population (e.g., substantially pure). Conversely, the cell population may contain up to 50% cells other than HCECs (e.g., keratocytes, etc.), for example, 50% or less of such cells, preferably 25% or less of such cells, more preferably 10% or less of such cells, or even 5% or less of such cells.

[0107] The enriched cell population may be used immediately or may be stored, for example, at room temperature, 4°C, 37°C, or the cells may be frozen at liquid nitrogen temperatures for long-term storage.

[0108] In some embodiments, the enriched cells may be further expanded in vitro by adding culture medium, as widely described in the literature (see, e.g., Li W et al., Invest Ophthalmol Vis Sci 2007; 48: 614.; Ishino Y et al., Invest Ophthalmol Vis Sci 2004; 45: 800; Chen KH et al., Cornea 2001; 20: 731).

[0109] The concentrated HCEC composition thus obtained has a variety of uses in clinical treatment, research, development, and commercial purposes.

[0110] For example, for therapeutic purposes, human corneal endothelial cells may be administered to a patient's eye to treat corneal endothelial cell loss or dysfunction.

[0111] Another aspect of the present invention relates to kits for performing cell separations as described herein. Such kits may include, among other elements, any combination of the following: (a) one, two, three, or more positive affinity reagents, each of which may be in the form of a positive affinity antibody attached to a suitable substrate, such as a solid matrix (e.g., a plate, immobilized beads, etc.) or a label (e.g., a magnetic label, a fluorescent label, etc.); (b) one, two, three, or more unlabeled positive affinity antibodies, which the end user may select and label using standard methods with a preferred label (e.g., a fluorophore, a hapten, etc.); and (c) one, two, three, or more unlabeled positive affinity antibodies, each of which may be attached to a suitable substrate, such as a solid matrix (e.g., a plate, immobilized beads, etc.) or a label (e.g., a magnetic label, a fluorescent label, etc.). (d) one, two, three, or more negative affinity reagents, which may be in the form of negative affinity antibodies attached to a suitable substrate such as a fluorophore, fluorescent label, etc.; (e) one, two, three, or more unlabeled negative affinity antibodies, which the end user can label using standard methods with a preferred label (e.g., a fluorophore, hapten, etc.); (e) a combination of (a) and (c); (f) a combination of (b) and (d); (g) packaging; (h) printed material with one or more of the following: (i) storage information, and (ii) instructions for use of the materials included in the kit (e.g., positive affinity reagents, negative affinity reagents, antibody combinations for sequential use, etc.). [Example]

[0112] HCECs were isolated from cadaveric donor corneas (Tampa Lions Eye Bank) and cultured and expanded according to the method described by Joyce and Zhu in Cornea. 2004 Nov;23(8 Suppl):S8-S19. Briefly, the endothelium and Descemet's membrane were stripped from the stroma and stabilized overnight at 37°C in Opti-MEM® medium (Gibco, Life Technologies, Carlsbad, CA) supplemented with 8% fetal bovine serum (FBS). After stabilization, the cells were incubated for 1 hour at 37°C with ethylenediaminetetraacetic acid (EDTA) to reduce cell-cell interactions. Next, the cells were mechanically dissociated to obtain single-cell suspensions and seeded onto FNC-coated culture wells, classified as "P0" (passage 0). After reaching confluence, the cells were trypsinized and further expanded into more wells to increase cell numbers. After one or two rounds of expansion, cells were harvested and cultured with different antibodies, as described below. Keratocytes were also obtained from cadaveric donor corneas using the method described by Stramer et al. in "Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes," Invest. Ophthalmol. Vis. Sci. 2004 vol. 45 no. 3 pp. 807-812. One of the HCEC cultures maintained the typical cobblestone morphology at passage 2 (Figure 1A), while the second culture underwent endothelial-to-mesenchymal transition during passage 3 (P3), with the cells becoming fibroblastic (Figure 1B). These cells are generally referred to herein as "less useful" human corneal endothelial cells (e.g., fibroblastic or mesenchymal-transformed HCECs). Keratocyte cultures exhibit a typical fibroblast-like, elongated cell morphology (Fig. 1C).

[0113] HCECs and keratocytes from each culture were collected and incubated with one or more of the following labeled antibodies: (a) APC-CD56, a mouse monoclonal antibody against the protein product of gene X15 in Table 2 (referred to herein as CD56 surface protein), conjugated to allophycocyanin (BD Biosciences no. 555518); (b) phycoerythrin (BD (c) FITC-CAR, a murine monoclonal antibody against the protein product of gene X1 in Table 2 (referred to herein as CD166 surface protein) conjugated to fluorescein-5-isothiocyanate (BD Biotechnology, Inc., Santa Cruz, CA, USA, no. sc-56892); and (d) PECy7-CD90, a murine monoclonal antibody against the protein product of gene Z8 in Table 2 (referred to herein as CD90 surface protein) conjugated to a tandem conjugate of PE (energy donor) having an excitation wavelength of 565 nm and Cy7 (energy acceptor) having an emission wavelength of 778 nm (BD Biosciences no. 561558).

[0114] Surface marker expression was measured using BD LSR (BD Biosciences, San Jose, CA). TM The cells were analyzed using a NIH II flow cytometry system. Data shown in Figure 2 are representative of one experiment. Similar results were obtained in repeated experiments. Quantification of the % of cells positive for each marker revealed decreased expression of CD56 and CAR in fibroblast-like cultures, indicating that antibodies against these proteins may be used in conjunction with positive affinity reagents for "good" HCECs. No significant differences in CD166 or CD90 expression were detected using this specific antibody.

[0115] Figures 3A-3C are two-color fluorescent dot plots of HCECs and keratocytes. These dot plots demonstrate the differential expression of two surface markers in each cell population, as labeled. The percentage of cells positive for each marker is presented in Figure 2. [Example]

[0116] HCECs were isolated from cadaveric donor corneas as described in Example 1. Also as described in Example 1, HCEC cultures were obtained in which (a) they clearly exhibited typical cobblestone morphology (referred to in this Example as "canonical" cell cultures), (b) all cells underwent endothelial-to-mesenchymal transition (referred to in this Example as "fibroblast-like" cell cultures), and (c) some HCECs underwent endothelial-to-mesenchymal transition (referred to in this Example as "mixed" cell cultures).

[0117] HCEC surface markers were identified by microarray data, and some showed high expression in the endothelium (cultured and freshly dissected), but low expression in the stroma were selected for testing by flow cytometry analysis. In addition to APC-CD56, PE-CD166, FITC-CAR, and PECy7-CD90 described in Example 1, (e) CD109-PE (i.e., mouse anti-CD109), a monoclonal antibody against the protein product of gene Y6 in Table 2 (called the CD109 antigen) conjugated to phycoerythrin (PE), BD Biosciences, catalog number 556040, and (f) CD248-BV (i.e., mouse anti-endosialin), a non-conjugated monoclonal antibody against the protein product of gene X5 in Table 2 (called the CD248 antigen or endosialin), (Millipore, Temecula, CA, catalog number MAB2626), incubated with a goat polyclonal anti-mouse IgG secondary antibody conjugated to Brilliant Violent 421 (Biolegend, San Diego, CA, catalog number 405317), were also tested.

[0118] To address whether the expression of these markers in HCECs was affected by the fibroblastic conversion described above, HCEC cultures exhibiting two distinct morphologies (canonical and fibroblastic) and keratocytes as controls were immunostained for the surface proteins CD90, CAR, CD56, and CD166 (see Example 1, Figure 2). Expression of CD56, CAR, CD109, and CD248 was also compared between canonical (good), mixed, and fibroblastic HCECs (see Figures 4 and 5). Analysis of the percentage of cells expressing any of the individual markers in canonical and fibroblastic cultures showed that expression of CD56, CAR, and CD248 was decreased in fibroblastic cultures (see Figure 5), while CD109 was increased (see Figure 5); expression of CD90 and CD166 did not change significantly between good / canonical and fibroblastic cultures (see Example 1, Figure 2). Comparable trends were observed in keratocyte cultures used as controls for expression of CD90, CAR, CD56, and CD166 (see Example 1, Figure 2).

[0119] Dot plot two-dimensional histograms of canonical, mixed, and fibroblast-like cultures, presented in Figure 6, showed that canonical HCECs were primarily CD56, CD248, and CAR positive and CD109 negative; as the cultures became more fibroblast-like, expression of CD56 and CD248 was lost and expression of CD109 increased.

[0120] Finally, the transendothelial electrical resistance (TEER) of the cell cultures was measured. HCECs from (a) "good" or "canonical" cultures expressing high levels of CD56, (b) mixed cultures, and (c) fibroblast-like cultures were plated at a density of 20,000 cells / insert onto 0.4 mm pore inserts in 24-well culture dishes (Costar Transwell, Corning, Acton, MA, USA) and cultured in Growth Medium as described in Example 1. TEER was measured up to 65 days after initial plating using an EVOM volt-ohm meter (World Precision Instruments, Sarasota, FL) with an STX2 electrode. TEER measures the resistance of the apical and basal membranes, as well as the paracellular resistance, and is used as an indicator of the integrity of the tight junction monolayer confluence. To calculate the final resistance (Ω·cm), the resistance of the blank filter was subtracted from the resistance of the filter containing cells. Four wells per condition were averaged. HCECs that displayed canonical morphology and were CD56-positive exhibited excellent barrier-forming ability as measured by TEER (FIG. 7).

[0121] We therefore identified a panel of surface markers that can be used to characterize canonical and functionally superior HCEC cultures and potentially be used as quality control measures or to isolate HCEC subpopulations optimal for expansion.

[0122] While various embodiments have been specifically illustrated and described herein, it will be understood that modifications and variations of the invention are encompassed by the above teachings and may lie within the purview of any appended claims without departing from the spirit and intended scope of the invention.

[0123] Table 2 Gene ID: X1 Gene symbol: ALCAM Gene description: activated leukocyte cell adhesion molecule Unigene: Hs.591293 Genbank: DQ486139 Entrez Gene: 214 Refseq: NM_001627 Protein sequence (SEQ ID NO: 1): 1 meskgasscr llfcllisat vfrpglgwyt vnsaygdtii ipcrldvpqn lmfgkwkyek 61 pdgspvfiaf rsstkksvqy ddvpeykdrl nlsenytlsi snarisdekr fvcmlvtedn 121 vfeaptivkv fkqpskpeiv skalfleteq lkklgdcise dsypdgnitw yrngkvlhpl 181 egavviifkk emdpvtqlyt mtstleyktt kadiqmpftc svtyygpsgq ktihseqavf 241 diyypteqvt iqvlppknai kegdnitlkc lgngnpppee flfylpgqpe girssntytl 301 tdvrrnatgd ykcslidkks miastaitvh yldlslnpsg evtrqigdal pvsctisasr 361 natvvwmkdn irlrsspsfs slhyqdagny vcetalqeve glkkresltl ivegkpqikm 421 tkktdpsgls ktiichvegf pkpaiqwtit gsgsvinqte espyingryy skiiispeen 481 vtltctaenq lertvnslnv saisipehde adeisdenre kvndqakliv givvglllaa 541 lvagvvywly mkksktaskh vnkdlgnmee nkkleennhk tea

[0124] Gene ID: X2 Gene symbol: ATP1A1 Gene description: Sodium / potassium transport ATPase subunit alpha-1 Unigene: Hs.371889 Genbank: BC003077 Entrez Gene: 476 Refseq: NM_000701|NM_001160233|NM_001160234 Protein sequence isoform a (SEQ ID NO: 2): 1 mgkgvgrdky epaavseqgd kkgkkgkkdr dmdelkkevs mddhklslde lhrkygtdls 61 rgltsaraae ilardgpnal tpppttpewi kfcrqlfggf smllwigail cflaysiqaa 121 teeepqndnl ylgvvlsavv iitgcfsyyq eaksskimes fknmvpqqal virngekmsi 181 naeevvvgdl vevkggdrip adlriisang ckvdnssltg esepqtrspd ftnenpletr 241 niaffstncv egtargivvy tgdrtvmgri atlasglegg qtpiaaeieh fihiitgvav 301 flgvsffils lileytwlea vifligiiva nvpegllatv tvcltltakr marknclvkn 361 leavetlgst sticsdktgt ltqnrmtvah mwfdnqihea dttenqsgvs fdktsatwla 421 lsriaglcnr avfqanqenl pilkravagd asesallkci elccgsvkem reryakivei 481 pfnstnkyql sihknpntse pqhllvmkga perildrcss illhgkeqpl deelkdafqn 541 aylelgglge rvlgfchlfl pdeqfpegfq fdtddvnfpi dnlcfvglis midppraavp 601 davgkcrsag ikvimvtgdh pitakaiakg vgiisegnet vediaarlni pvsqvnprda 661 kacvvhgsdl kdmtseqldd ilkyhteivf artspqqkli ivegcqrqga ivavtgdgvn 721 dspalkkadi gvamgiagsd vskqaadmil lddnfasivt gveegrlifd nlkksiaytl 781 tsnipeitpf lifiianipl plgtvtilci dlgtdmvpai slayeqaesd imkrqprnpk 841 tdklvnerli smaygqigmi qalggfftyf vilaengflp ihllglrvdw ddrwindved 901 sygqqwtyeq rkiveftcht affvsivvvq wadlvicktr rnsvfqqgmk nkilifglfe 961 etalaaflsy cpgmgvalrm yplkptwwfc afpysllifv ydevrkliir rrpggwveke 1021 tyy

[0125] Protein sequence isoform c (SEQ ID NO: 3): 1 mafkvgrdky epaavseqgd kkgkkgkkdr dmdelkkevs mddhklslde lhrkygtdls 61 rgltsaraae ilardgpnal tpppttpewi kfcrqlfggf smllwigail cflaysiqaa 121 teeepqndnl ylgvvlsavv iitgcfsyyq eaksskimes fknmvpqqal virngekmsi 181 naeevvvgdl vevkggdrip adlrisiang ckvdnssltg esepqtrspd ftnenpletr 241 niaffstncv egtargivvy tgdrtvmgri atlasglegg qtpiaaeieh fihiitgvav 301 flgvsffils lileytwlea vifligiiva nvpegllatv tvcltltakr marknclvkn 361 leavetlgst sticsdktgt ltqnrmtvah mwfdnqihea dttenqsgvs fdktsatwla 421 lsriaglcnr avfqanqenl pilkravagd asesallkci elccgsvkem reryakivei 481 pfnstnkyql sihknpntse pqhllvmkga perildrcss illhgkeqpl deelkdafqn 541 aylelgglge rvlgfchlfl pdeqpfegfq fdtddvnfpi dnlcfvglis midppraavp 601 davgkcrsag ikvimvtgdh pitakaiakg vgiisegnet vediaarlni pvsqvnprda 661 kacvvhgsdl kdmtseqldd ilkyhteivf artspqqkli ivegcqrqga ivavtgdgvn 721 dspalkkadi gvamgiagsd vskqaadmil lddnfasivt gveegrlifd nlkksiaytl 781 tsnipeitpf lifiianipl plgtvtilci dlgtdmvpai slayeqaesd imkrqprnpk 841 tdklvnerli smaygqigmi qalggfftyf vilaengflp ihllglrvdw ddrwindved 901 sygqqwtyeq rkiveftcht affvsivvvq wadlvicktr rnsvfqqgmk nkilifglfe 961 etalaaflsy cpgmgvalrm yplkptwwfc afpysllifv ydevrkliir rrpggwveke 1021 tyy

[0126] Protein sequence isoform d (SEQ ID NO: 4): 1 mdelkkevsm ddhklsldel hrkygtdlsr gltsaraaei lardgpnalt pppttpewik 61 fcrqlfggfs mllwigailc flaysiqaat eeepqndnly lgvvlsavvi itgcfsyyqe 121 aksskimesf knmvpqqalv irngekmsin aeevvvgdlv evkggdripa dlriisangc 181 kvdnssltge sepqtrspdf tnenpletrn iaffstncve gtargivvyt gdrtvmgria 241 tlasgleggq tpiaaeiehf ihiitgvavf lgvsffilsl ileytwleav ifligiivan 301 vpegllatvt vcltltakrm arknclvknl eavetlgsts ticsdktgtl tqnrmtvahm 361 wfdnqihead ttenqsgvsf dktsatwlal sriaglcnra vfqanqenlp ilkravagda 421 sesallkcie lccgsvkemr eryakiveip fnstnkyqls ihknpntsep qhllvmkgap 481 erildrcssi llhgkeqpld eelkdafqna ylelgglger vlgfchlflp deqfpegfqf 541 dtddvnfpid nlcfvglism idppraavpd avgkcrsagi kvimvtgdhp itakaiakgv 601 giisegnetv ediaarlnip vsqvnprdak acvvhgsdlk dmtseqlddi lkyhteivfa 661 rtspqqklii vegcqrqgai vavtgdgvnd spalkkadig vamgiagsdv skqaadmill 721 ddnfasivtg veegrlifdn lkksiaytlt snipeitpfl ifiianiplp lgtvtilcid 781 lgtdmvpais layeqaesdi mkrqprnpkt dklvnerlis maygqigmiq alggfftyfv 841 ilaengflpi hllglrvdwd drwindveds ygqqwtyeqr kiveftchta ffvsivvvqw 901 adlvicktrr nsvfqqgmkn kilifglfee talaaflsyc pgmgvalrmy plkptwwfca 961 fpysllifvy devrkliirr rpggwveket yy

[0127] Gene ID: X3 Gene symbol: CD200 Gene description: CD 200 molecule Unigene: Hs.79015 Genbank: AK297194|AF063591|BC022522|BC031103|AY603771|AK293399 Entrez Gene: 4345 Refseq: NM_001004196|NM_005944 Protein sequence isoform b (SEQ ID NO: 5): 1 merltltrti ggplltatll gkttindyqv irmpfshlst yslvwvmaav vlctaqvqvv 61 tqdereqlyt paslkcslqn aqealivtwq kkkavspenm vtfsenhgvv iqpaykdkin 121 itqlglqnst itfwnitled egcymclfnt fgfgkisgta cltvyvqpiv slhykfsedh 181 lnitcsatar papmvfwkvp rsgienstvt lshpngttsv tsilhikdpk nqvgkevicq 241 vlhlgtvtdf kqtvnkgywf svplllsivs lvillvlisi llywkrhrnq drep

[0128] Protein sequence isoform a (SEQ ID NO: 6): 1 merlvirmpf shlstyslvw vmaavvlcta qvqvvtqder eqlytpaslk cslqnaqeal 61 ivtwqkkkav spenmvtfse nhgvviqpay kdkinitqlg lqnstitfwn itledegcym 121 clfntfgfgk isgtacltvy vqpivslhyk fsedhlnitc satarpapmv fwkvprsgie 181 nstvtlshpn gttsvtsilh ikdpknqvgk evicqvlhlg tvtdfkqtvn kgywfsvpll 241 lsivslvill vlisillywk rhrnqdrep

[0129] Gene ID: X4 Gene symbol: LAMB1 Gene description: Laminin, beta 1 Unigene: Hs.650585 Genbank: M61916 Entrez Gene: 3912 Refseq: NM_002291 Protein sequence (SEQ ID NO: 7): 1 mgllqllafs flalcrarvr aqepefsygc aegscypatg dlligraqkl svtstcglhk 61 pepycivshl qedkkcficn sqdpyhetln pdshlienvv ttfapnrlki wwqsengven 121 vtiqldleae fhfthlimtf ktfrpaamli erssdfgktw gvyryfaydc easfpgistg 181 pmkkvddiic dsrysdieps tegevifral dpafkiedpy spriqnllki tnlrikfvkl 241 htlgdnllds rmeirekyyy avydmvvrgn cfcyghasec apvdgfneev egmvhghcmc 301 rhntkglnce lcmdfyhdlp wrpaegrnsn ackkcncneh sischfdmav ylatgnvsgg 361 vcddcqhntm grnceqckpf yyqhperdir dpnfcerctc dpagsqnegi cdsytdfstg 421 liagqcrckl nvegehcdvc kegfydlsse dpfgckscac nplgtipggn pcdsetghcy 481 ckrlvtgqhc dqclpehwgl sndldgcrpc dcdlggalnn scfaesgqcs crphmigrqc 541 nevepgyyfa tldhylyeae eanlgpgvsi verqyiqdri pswtgagfvr vpegayleff 601 idnipysmey diliryepql pdhwekavit vqrpgripts srcgntipdd dnqvvslspg 661 sryvvlprpv cfekgtnytv rlelpqytss dsdvespytl idslvlmpyc ksldiftvgg 721 sgdgvvtnsa wetfqryrcl ensrsvvktp mtdvcrniif sisallhqtg lacecdpqgs 781 lssvcdpngg qcqcrpnvvg rtcnrcapgt fgfgpsgckp cechlqgsvn afcnpvtgqc 841 hcfqgvyarq cdrclpghwg fpscqpcqcn ghaddcdpvt geclncqdyt mghncercla 901 gyygdpiigs gdhcrpcpcp dgpdsgrqfa rscyqdpvtl qlacvcdpgy igsrcddcas 961 gyfgnpsevg gscqpcqchn nidttdpeac dketgrclkc lyhtegehcq fcrfgyygda 1021 lqqdcrkcvc nylgtvqehc ngsdcqcdka tgqclclpnv igqncdrcap ntwqlasgtg 1081 cdpcncnaah sfgpscneft gqcqcmpgfg grtcsecqel fwgdpdvecr acdcdprgie 1141 tpqcdqstgq cvcvegvegp rcdkctrgys gvfpdctpch qcfalwdvii aeltnrthrf 1201 lekakalkis gvigpyretv dsverkvsei kdilaqspaa eplknignlf eeaeklikdv 1261 temmaqvevk lsdttsqsns takeldslqt eaesldntvk elaeqlefik nsdirgalds 1321 itkyfqmsle aeervnastt epnstveqsa lmrdrvedvm meresqfkek qeeqarllde 1381 lagklqsldl saaaemtcgt ppgascsete cggpncrtde gerkcggpgc gglvtvahna 1441 wqkamdldqd vlsalaeveq lskmvseakl radeakqsae dillktnatk ekmdksneel 1501 rnlikqirnf ltqdsadlds ieavanevlk mempstpqql qnltedirer veslsqvevi 1561 lqhsaadiar aemlleeakr asksatdvkv tadmvkeale eaekaqvaae kaikqadedi 1621 qgtqnlltsi estaaseet lfnasqrise lernveelkr kaaqnsgeae yiekvvytvk 1681 qsaedvkktl dgeldekykk venliakkte esadarrkae mlqneaktll aqansklqll 1741 kdlerkyedn qryledkaqe larlegevrs llkdisqkva vystcl

[0130] Gene ID: X5 Gene symbol: CD248 Gene description: endosialin Unigene: Hs.195727 Genbank: AF279142 Entrez Gene: 57124 Refseq: NM_020404 Protein sequence (SEQ ID NO: 8): 1 mllrlllawa aagptlgqdp waaepraacg psscyalfpr rrtfleawra crelggdlat 61 prtpeeaqrv dslvgagpas rllwiglqrq arqcqlqrpl rgftwttgdq dtaftnwaqp 121 asggpcpaqr cvaleasgeh rwlegsctla vdgylcqfgf egacpalqde agqagpavyt 181 tpfhlvstef ewlpfgsvaa vqcqagrgas llcvkqpegg vgwsragplc lgtgcspdng 241 gcehecveev dghvscrcte gfrlaadgrs cedpcaqapc eqqcepggpq gyschcrlgf 301 rpaeddphrc vdtdecqiag vcqqmcvnyv ggfecycseg heleadgisc spagamgaqa 361 sqdlgdelld dgedeedede awkafnggwt empgilwmep tqppdfalay rpsfpedrep 421 qipypeptwp pplsaprvpy hssvlsvtrp vvvsathptl psahqppvip athpalsrdh 481 qipviaanyp dlpsayqpgi lsvshsaqpp ahqppmistk ypelfpahqs pmfpdtrvag 541 tqttthlpgi ppnhaplvtt lgaqlppqap dalvlrtqat qlpiiptaqp sltttsrspv 601 spahqisvpa atqpaalptl lpsqsptnqt spispthphs kapqipredg pspklalwlp 661 spaptaapta lgeaglaehs qrddrwllva llvptcvflv vllalgivyc trcgphapnk 721 ritdcyrwvi hagsksptep mpprgsltgv qtcrtsv

[0131] Gene ID: X6 Gene symbol: COL4A6 Gene Description: Collagen, Type IV, Alpha 6 Unigene: Hs.145586 Genbank: D21337 Entrez Gene: 1288 Refseq: NM_033641|NM_001847 Protein sequence isoform b (SEQ ID NO: 9): 1 mhpglwlllv tlclteelaa ageksygkpc ggqdcsgscq cfpekgargr pgpigiqgpt 61 gpqgftgstg lsglkgergf pgllgpygpk gdkgpmgvpg flgingipgh pgqpgprgpp 121 gldgcngtqg avgfpgpdgy pgllgppglp gqkgskgdpv lapgsfkgmk gdpglpgldg 181 itgpqgapgf pgavgpagpp glqgppgppg plgpdgnmgl gfqgekgvkg dvglpgpagp 241 ppstgelefm gfpkgkkgsk gepgpkgfpg isgppgfpgl gttgekgekg ekgipglpgp 301 rgpmgsegvq gppgqqgkkg tlgfpglngf qgiegqkgdi glpgpdvfid idgavisgnp 361 gdpgvpglpg lkgdegiqgl rgpsgvpglp alsgvpgalg pqgfpglkgd qgnpgrttig 421 aaglpgrdgl pgppgppgpp spefetetlh nkesgfpglr geqgpkgnlg lkgikgdsgf 481 cacdggvpnt gppgepgppg pwgliglpgl kgargdrgsg gaqgpagapg lvgplgpsgp 541 kgkkgepils tiqgmpgdrg dsgsqgfrgv igepgkdgvp glpglpglpg dggqgfpgek 601 glpglpgekg hpgppglpgn glpglpgprg lpgdkgkdgl pgqqglpgsk gitlpciipg 661 sygpsgfpgt pgfpgpkgsr glpgtpgqpg ssgskgepgs pglvhlpelp gfpgprgekg 721 lpgfpglpgk dglpgmigsp glpgskgatg difgaengap geqglqgltg hkgflgdsgl 781 pglkgvhgkp gllgpkgerg spgtpgqvgq pgtpgssgpy gikgksglpg apgfpgisgh 841 pgkkgtrgkk gppgsivkkg lpglkglpgn pglvglkgsp gspgvaglpa lsgpkgekgs 901 vgfvgfpgip glpgipgtrg lkgipgstgk mgpsgragtp gekgdrgnpg pvgipsprrp 961 msnlwlkgdk gsqgsagsng fpgprgdkge agrpgppglp gapglpgiik gvsgkpgppg 1021 fmgirglpgl kgssgitgfp gmpgesgsqg irgspglpga sglpglkgdn gqtveisgsp 1081 gpkgqpgesg fkgtkgrdgl ignigfpgnk gedgkvgvsg dvglpgapgf pgvagmrgep 1141 glpgssghqg aigplgspgl igpkgfpgfp glhglnglpg tkgthgtpgp sitgvpgpag 1201 lpgpkgekgy pgigigapgk pglrgqkgdr gfpglqgpag lpgapgislp sliagqpgdp 1261 grpgldgerg rpgpagppgp pgpssnqgdt gdpgfpgipg pkgpkgdqgi pgfsglpgel 1321 glkgmrgepg fmgtpgkvgp pgdpgfpgmk gkagprgssg lqgdpgqtpt aeavqvppgp 1381 lglpgidgip gltgdpgaqg pvglqgskgl pgipgkdgps glpgppgalg dpglpglqgp 1441 pgfegapgqq gpfgmpgmpg qsmrvgytlv khsqseqvpp cpigmsqlwv gysllfvegq 1501 ekahnqdlgf agsclprfst mpfiycnine vchyarrndk sywlsttapi pmmpvsqtqi 1561 pqyisrcsvc eapsqaiavh sqditipqcp lgwrslwigy sflmhtaaga egggqslvsp 1621 gscledfrat pfiecsgarg tchyfankys fwlttveerq qfgelpvset lkagqlhtrv 1681 srcqvcmksl

[0132] Protein sequence isoform a (SEQ ID NO: 10): 1 mlinklwlll vtlclteela aageksygkp cggqdcsgsc qcfpekgarg rpgpigiqgp 61 tgpqgftgst glsglkgerg fpgllgpygp kgdkgpmgvp gflgingipg hpgqpgprgp 121 pgldgcngtq gavgfpgpdg ypgllgppgl pgqkgskgdp vlapgsfkgm kgdpglpgld 181 gitgpqgapg fpgavgpagp pglqgppgpp gplgpdgnmg lgfqgekgvk gdvglpgpag 241 pppstgelef mgfpkgkkgs kgepgpkgfp gisgppgfpg lgttgekgek gekgipglpg 301 prgpmgsegv qgppgqqgkk gtlgfpglng fqgiegqkgd iglpgpdvfi didgavisgn 361 pgdpgvpglp glkgdegiqg lrgpsgvpgl palsgvpgal gpqgfpglkg dqgnpgrtti 421 gaaglpgrdg lpgppgppgp pspefetetl hnkesgfpgl rgeqgpkgnl glkgikgdsg 481 fcacdggvpn tgppgepgpp gpwgliglpg lkgargdrgs ggaqgpagap glvgplgpsg 541 pkgkkgepil stiqgmpgdr gdsgsqgfrg vigepgkdgv pglpglpglp gdggqgfpge 601 kglpglpgek ghpgppglpg nglpglpgpr glpgdkgkdg lpgqqglpgs kgitlpciip 661 gsygpsgfpg tpgfpgpkgs rglpgtpgqp gssgskgepg spglvhlpel pgfpgprgek 721 glpgfpglpg kdglpgmigs pglpgskgat gdifgaenga pgeqglqglt ghkgflgdsg 781 lpglkgvhgk pgllgpkger gspgtpgqvg qpgtpgssgp ygikgksglp gapgfpgisg 841 hpgkkgtrgk kgppgsivkk glpglkglpg npglvglkgs pgspgvaglp alsgpkgekg 901 svgfvgfpgi pglpgipgtr glkgipgstg kmgpsgragt pgekgdrgnp gpvgipsprr 961 pmsnlwlkgd kgsqgsagsn gfpgprgdkg eagrpgppgl pgapglpgii kgvsgkpgpp 1021 gfmgirglpg lkgssgitgf pgmpgesgsq girgspglpg asglpglkgd ngqtveisgs 1081 pgpkgqpges gfkgtkgrdg lignigfpgn kgedgkvgvs gdvglpgapg fpgvagmrge 1141 pglpgssghq gaigplgspg ligpkgfpgf pglhglnglp gtkgthgtpg psitgvpgpa 1201 glpgpkgekg ypgigigapg kpglrgqkgd rgfpglqgpa glpgapgisl psliagqpgd 1261 pgrpgldger grpgpagppg ppgpssnqgd tgdpgfpgip gpkgpkgdqg ipgfsglpge 1321 lglkgmrgep gfmgtpgkvg ppgdpgfpgm kgkagprgss glqgdpgqtp taeavqvppg 1381 plglpgidgi pgltgdpgaq gpvglqgskg lpgipgkdgp sglpgppgal gdpglpglqg 1441 ppgfegapgq qgpfgmpgmp gqsmrvgytl vkhsqseqvp pcpigmsqlw vgysllfveg 1501 qekahnqdlg fagsclprfs tmpfiycnin evchyarrnd ksywlsttap ipmmpvsqtq 1561 ipqyisrcsv ceapsqaiav hsqditipqc plgwrslwig ysflmhtaag aegggqslvs 1621 pgscledfra tpfiecsgar gtchyfanky sfwlttveer qqfgelpvse tlkagqlhtr 1681 vsrcqvcmks l

[0133] Gene ID: X7 Gene symbol: PCDH7 Gene description: protocadherin 7 Unigene: Hs.479439|Hs.724529 Genbank: AB006755 Entrez Gene: 5099 Refseq: NM_032456|NM_002589|NM_032457|NM_001173523 Protein sequence isoform b (SEQ ID NO: 11): 1 mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg 61 sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps 121 qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery 181 ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr 241 ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd 301 vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl 361 detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki 421 griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq 481 nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv 541 evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre 601 qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm 661 vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp 721 prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln 781 ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn 841 esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm 901 arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn 961 gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021 vqdlppantf vgagdnisig sdhcseyscq tnnkyskqvr cipnifkypr eg

[0134] Protein sequence isoform a (SEQ ID NO: 12): 1 mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg 61 sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps 121 qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery 181 ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr 241 ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd 301 vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl 361 detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki 421 griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq 481 nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv 541 evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre 601 qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm 661 vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp 721 prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln 781 ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn 841 esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm 901 arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn 961 gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021 vqdlppantf vgagdnisig sdhcseyscq tnnkyskqmr lhpyitvfg

[0135] Protein sequence isoform c (SEQ ID NO: 13): 1 mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg 61 sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps 121 qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery 181 ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr 241 ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd 301 vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl 361 detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki 421 griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq 481 nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv 541 evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre 601 qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm 661 vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp 721 prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln 781 ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn 841 esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm 901 arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn 961 gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021 vqdlppantf vgagdnisig sdhcseyscq tnnkyskqpf rrvtfsvvsq pqdphqgslq 1081 scydsglees etpssksssg prlgalplpe dnyerttpdg svdsrplpdv altgkctrec 1141 deyghsdscw mpvrtsperk ksqpklstfm pvdergsqek langeaaimg drnrnllnkk 1201 ltssyetfsa asfskneean pedipltktg eykpspvntl trrevyl

[0136] Protein sequence isoform d (SEQ ID NO: 14): 1 mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg 61 sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps 121 qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery 181 ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr 241 ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd 301 vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl 361 detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki 421 griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq 481 nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv 541 evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre 601 qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm 661 vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp 721 prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln 781 ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn 841 esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm 901 arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn 961 gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021 vqdlppantf vgagdnisig sdhcseyscq tnnkyskqpf rrvtfsvvsq pqdphqgslq 1081 scydsglees etpssksssg prlgalplpe dnyerttpdg svgeaehmen dsrplpdval 1141 tgkctrecde yghsdscwmp vrtsperkks qpklstfmpv dergsqekla ngeaaimgdr 1201 nrnllnkklt ssyetfsaas fskneeanpe dipltktgey kpspvntltr revyl

[0137] Gene ID: X8 Gene symbol: NOG Gene description: noggin Unigene: Hs.248201 Genbank: BC034027 Entrez Gene: 9241 Refseq: NM_005450 Protein sequence (SEQ ID NO: 15): 1 mercpslgvt lyalvvvlgl ratpaggqhy lhirpapsdn lplvdliehp dpifdpkekd 61 lnetllrsll gghydpgfma tsppedrpgg gggaaggaed laeldqllrq rpsgampsei 121 kglefsegla qgkkqrlskk lrrklqmwlw sqtfcpvlya wndlgsrfwp ryvkvgscfs 181 krscsvpegm vckpsksvhl tvlrwrcqrr ggqrcgwipi qypiiseckc sc

[0138] Gene ID: X9 Gene symbol: SULF1 Gene description: sulfatase 1 Unigene: Hs.409602 Genbank: AF545571 Entrez Gene: 23213 Refseq: NM_001128205|NM_015170|NM_001128206|NM_001128204 Protein sequence (SEQ ID NO: 16): 1 mkysccalvl avlgtellgs lcstvrsprf rgriqqerkn irpniilvlt ddqdvelgsl 61 qvmnktrkim ehggatfina fvttpmccps rssmltgkyv hnhnvytnne ncsspswqam 121 heprtfavyl nntgyrtaff gkylneyngs yippgwrewl gliknsrfyn ytvcrngike 181 khgfdyakdy ftdlitnesi nyfkmskrmy phrpvmmvis haaphgpeds apqfsklypn 241 asqhitpsyn yapnmdkhwi mqytgpmlpi hmeftnilqr krlqtlmsvd dsverlynml 301 vetgelenty iiytadhgyh igqfglvkgk smpydfdirv pffirgpsve pgsivpqivl 361 nidlaptild iagldtppdv dgksvlklld pekpgnrfrt nkkakiwrdt flvergkflr 421 kkeesskniq qsnhlpkyer vkelcqqary qtaceqpgqk wqciedtsgk lrihkckgps 481 dlltvrqstr nlyargfhdk dkecscresg yrasrsqrks qrqflrnqgt pkykprfvht 541 rqtrslsvef egeiydinle eeeelqvlqp rniakrhdeg hkgprdlqas sggnrgrmla 601 dssnavgppt tvrvthkcfi lpndsihcer elyqsarawk dhkayidkei ealqdkiknl 661 revrghlkrr kpeecscskq syynkekgvk kqeklkshlh pfkeaaqevd sklqlfkenn 721 rrrkkerkek rrqrkgeecs lpgltcfthd nnhwqtapfw nlgsfcacts snnntywclr 781 tvnethnflf cefatgfley fdmntdpyql tntvhtverg ilnqlhvqlm elrscqgykq 841 cnprpknldv gnkdggsydl hrgqlwdgwe g

[0139] Gene ID: X10 Gene symbol: SORT1 Gene description: sortilin 1 Unigene: Hs.485195 Genbank: X98248 Entrez Gene: 6272 Refseq: NM_002959| NM_001205228 Protein Sequence Isoform 1 (SEQ ID NO: 17): 1 merpwgaadg lsrwphglgl llllqllpps tlsqdrldap pppaaplprw sgpigvswgl 61 raaaaggafp rggrwrrsap gedeecgrvr dfvaklannt hqhvfddlrg svslswvgds 121 tgvilvlttf hvplvimtfg qsklyrsedy gknfkditdl inntfirtef gmaigpensg 181 kvvltaevsg gsrggrifrs sdfaknfvqt dlpfhpltqm myspqnsdyl lalstenglw 241 vsknfggkwe eihkavclak wgsdntifft tyangsckad lgalelwrts dlgksfktig 301 vkiysfglgg rflfasvmad kdttrrihvs tdqgdtwsma qlpsvgqeqf ysilaanddm 361 vfmhvdepgd tgfgtiftsd drgivysksl drhlytttgg etdftnvtsl rgvyitsvls 421 ednsiqtmit fdqggrwthl rkpensecda taknknecsl hihasysisq klnvpmapls 481 epnavgivia hgsvgdaisv mvpdvyisdd ggyswtkmle gphyytilds ggiivaiehs 541 srpinvikfs tdegqcwqty tftrdpiyft glasepgars mnisiwgfte sfltsqwvsy 601 tidfkdiler nceekdytiw lahstdpedy edgcilgyke qflrlrkssv cqngrdyvvt 661 kqpsiclcsl edflcdfgyy rpendskcve qpelkghdle fclygreehl ttngyrkipg 721 dkcqggvnpv revkdlkkkc tsnflspekq nsksnsvpii laivglmlvt vvagvlivkk 781 yvcggrflvh rysvlqqhae angvdgvdal dtashtnksg yhddsdedll e

[0140] Protein Sequence Isoform 2 (SEQ ID NO: 18): 1 mtfgqsklyr sedygknfkd itdlinntfi rtefgmaigp ensgkvvlta evsggsrggr 61 ifrssdfakn fvqtdlpfhp ltqmmyspqn sdyllalste nglwvsknfg gkweeihkav 121 clakwgsdnt iffttyangs ctdlgalelw rtsdlgksfk tigvkiysfg lggrflfasv 181 madkdttrri hvstdqgdtw smaqlpsvgq eqfysilaan ddmvfmhvde pgdtgfgtif 241 tsddrgivys ksldrhlytt tggetdftnv tslrgvyits vlsednsiqt mitfdqggrw 301 thlrkpense cdataknkne cslhihasys isqklnvpma plsepnavgi viahgsvgda 361 isvmvpdvyi sddggyswtk mlegphyyti ldsggiivai ehssrpinvi kfstdegqcw 421 qtytftrdpi yftglasepg arsmnisiwg ftesfltsqw vsytidfkdi lernceekdy 481 tiwlahstdp edyedgcilg ykeqflrlrk ssvcqngrdy vvtkqpsicl csledflcdf 541 gyyrpendsk cveqpelkgh dlefclygre ehlttngyrk ipgdkcqggv npvrevkdlk 601 kkctsnflsp ekqnsksnsv piilaivglm lvtvvagvli vkkyvcggrf lvhrysvlqq 661 haeangvdgv daldtashtn ksgyhddsde dlle

[0141] Gene ID: X11 Gene symbol: ATP1B1 Gene description: Sodium / potassium-transporting ATPase subunit beta-1 Unigene: Hs.291196 Genbank: U16799 Entrez Gene: 481 Refseq: NM_001677 Protein sequence (SEQ ID NO: 19): 1 margkakeeg swkkfiwnse kkeflgrtgg swfkillfyv ifygclagif igtiqvmllt 61 isefkptyqd rvappgltqi pqiqkteisf rpndpksyea yvlnivrfle kykdsaqrdd 121 mifedcgdvp sepkergdfn hergerkvcr fklewlgncs glndetygyk egkpciiikl 181 nrvlgfkpkp pknesletyp vmkynpnvlp vqctgkrded kdkvgnveyf glgnspgfpl 241 qyypyygkll qpkylqplla vqftnltmdt eirieckayg enigysekdr fqgrfdvkie 301 vks

[0142] Gene ID: X12 Gene symbol: AGRN Gene description: Agrin Unigene: Hs.273330 Genbank: AB191264 Entrez Gene: 375790 Refseq: NM_198576 Protein sequence (SEQ ID NO: 20): 1 magrshpgpl rpllpllvva acvlpgaggt cperalerre eeanvvltgt veeilnvdpv 61 qhtysckvrv wrylkgkdlv areslldggn kvvisgfgdp licdnqvstg dtriffvnpa 121 ppylwpahkn elmlnsslmr itlrnleeve fcvedkpgth ftpvpptppd acrgmlcgfg 181 avcepnaegp grascvckks pcpsvvapvc gsdastysne celqraqcsq qrrirllsrg 241 pcgsrdpcsn vtcsfgstca rsadgltasc lcpatcrgap egtvcgsdga dypgecqllr 301 racarqenvf kkfdgpcdpc qgalpdpsrs crvnprtrrp emllrpescp arqapvcgdd 361 gvtyendcvm grsgaargll lqkvrsgqcq grdqcpepcr fnavclsrrg rprcscdrvt 421 cdgayrpvca qdgrtydsdc wrqqaecrqq raipskhqgp cdqapspclg vqcafgatca 481 vkngqaacec lqacsslydp vcgsdgvtyg saceleatac tlgreiqvar kgpcdrcgqc 541 rfgalceaet grcvcpsecv alaqpvcgsd ghtypsecml hvhacthqis lhvasagpce 601 tcgdavcafg avcsagqcvc prcehpppgp vcgsdgvtyg sacelreaac lqqtqieear 661 agpceqaecg sggsgsgedg dceqelcrqr ggiwdedsed gpcvcdfscq svpgspvcgs 721 dgvtystece lkkarcesqr glyvaaqgac rgptfaplpp vaplhcaqtp ygccqdnita 781 argvglagcp sacqcnphgs yggtcdpatg qcscrpgvgg lrcdrcepgf wnfrgivtdg 841 rsgctpcscd pqgavrddce qmtglcsckp gvagpkcgqc pdgralgpag ceadasapat 901 caemrcefga rcveesgsah cvcpmltcpe anatkvcgsd gvtygnecql ktiacrqglq 961 isiqslgpcq eavapsthpt sasvtvttpg lllsqalpap pgallappss tahsqttppp 1021 ssrprttasv prttvwpvlt vpptapspap slvasafges gstdgssdee lsgdqeasgg 1081 1141 egvegqelfy tpemadpkse lfgetarsie stlddlfrns dvkkdfrsvr lrdlgpgksv 1201 raivdvhfdp ttafrapdva rallrqiqvs rrrslgvrrp lqehvrfmdf dwfpafitga 1261 tsgaiaagat arattasrlp ssavtpraph pshtsqpvak ttaapttrrp pttapsrvpg 1321 rrppapqqpp kpcdsqpcfh ggtcqdwalg ggftcscpag rggavcekvl gapvpafegr 1381 sflafptlra yhtlrlalef ralepqglll yngnargkdf lalalldgrv qlrfdtgsgp 1441 avltsavpve pgqwhrlels rhwrrgtlsv dgetpvlges psgtdglnld tdlfvggvpe 1501 dqaavalert fvgaglrgci rlldvnnqrl elgigpgaat rgsgvgecgd hpclpnpchg 1561 gapcqnleag rfhcqcppgr vgptcadeks pcqpnpchga apcrvlpegg aqcecplgre 1621 gtfcqtasgq dgsgpfladf ngfshlelrg lhtfardlge kmalevvfla rgpsglllyn 1681 gqktdgkgdf vslalrdrrl efrydlgkga avirsrepvt lgawtrvsle rngrkgalrv 1741 gdgprvlges pvphtvlnlk eplyvggapd fsklaraaav ssgfdgaiql vslggrqllt 1801 pehvlrqvdv tsfaghpctr asghpclnga scvpreaayv clcpggfsgp hcekglveks 1861 agdvdtlafd grtfveylna vtesekalqs nhfelslrte atqglvlwsg kateradyva 1921 laivdghlql synlgsqpvv lrstvpvntn rwlrvvahre qregslqvgn eapvtgsspl 1981 gatqldtdga lwlgglpelp vgpalpkayg tgfvgclrdv vvgrhplhll edavtkpelr 2041 pcptp

[0143] Gene ID: X13 Gene symbol: APP Gene description: Amyloid beta A4 protein Unigene: Hs.434980 Genbank: BC065529|AF282245|AK298861|AK294534|AK295621|AK296229|AK297412| AK297229|AK295373|BC004369|M16765|AK311717 Entrez Gene: 351 Refseq: NM_000484|NM_201413|NM_001136130|NM_201414|NM_001136129 Protein sequence isoform a (SEQ ID NO: 21): 1 mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk 61 tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg 121 efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr 181 gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee 241 eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrev cseqaetgpc 301 ramisrwyfd vtegkcapff yggcggnrnn fdteeycmav cgsamsqsll kttqeplard 361 pvklpttaas tpdavdkyle tpgdenehah fqkakerlea khrermsqvm reweeaerqa 421 knlpkadkka viqhfqekve sleqeaaner qqlvethmar veamlndrrr lalenyital 481 qavpprprhv fnmlkkyvra eqkdrqhtlk hfehvrmvdp kkaaqirsqv mthlrviyer 541 mnqslsllyn vpavaeeiqd evdellqkeq nysddvlanm iseprisygn dalmpsltet 601 kttvellpvn gefslddlqp whsfgadsvp antenevepv darpaadrgl ttrpgsgltn 661 ikteeisevk mdaefrhdsg yevhhqklvf faedvgsnkg aiiglmvggv viatvivitl 721 vmlkkkqyts ihhgvvevda avtpeerhls kmqqngyenp tykffeqmqn

[0144] Protein sequence isoform b (SEQ ID NO: 22): 1 mlpglallll aawtaralev ptdgnaglalla epqiamfcgr lnmhmnvqng kwdsdpsgtk 61 tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg 121 efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr 181 gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee 241 eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrev cseqaetgpc 301 ramisrwyfd vtegkcapff yggcggnrnn fdteeycmav cgsaipttaa stpdavdkyl 361 etpgdeneha hfqkakerle akhrermsqv mreweeaerq aknlpkadkk aviqhfqekv 421 esleqeaane rqqlvethma rveamlndrr rlalenyita lqavppprprh vfnmlkkyvr 481 aeqkdrqhtl khfehvrmvd pkkaaqirsq vmthlrviye rmnqslslly nvpavaeeiq 541 devdellqke qnysddvlan miseprisyg ndalmpslte tkttvellpv ngefslddlq 601 pwhsfgadsv pantenevep vdarpaadrg lttrpgsglt nikteeisev kmdaefrhds 661 gyevhhqklv ffaedvgsnk gaiiglmvgg vviatvivit lvmlkkkqyt sihhgvvevd 721 aavtpeerhl skmqqngyen ptykffeqmq n

[0145] Protein sequence isoform f (SEQ ID NO: 23): 1 mlpglallll aawtaralev ypelqitnvv eanqpvtiqn wckrgrkqck thphfvipyr 61 clvgefvsda llvpdkckfl hqermdvcet hlhwhtvake tcsekstnlh dygmllpcgi 121 dkfrgvefvc cplaeesdnv dsadaeedds dvwwggadtd yadgsedkvv evaeeeeevae 181 veeeeaddde ddedgdevee eaeepyeeat erttsiattt ttttesveev vrevcseqae 241 tgpcramisr wyfdvtegkc apffyggcgg nrnnfdteey cmavcgsams qsllkttqep 301 lardpvklpt taastpdavd kyletpgden ehahfqkake rleakhrerm sqvmreweea 361 erqaknlpka dkkaviqhfq ekvesleqea anerqqlvet hmarveamln drrrlaleny 421 italqavppr prhvfnmlkk yvraeqkdrq htlkhfehvr mvdpkkaaqi rsqvmthlrv 481 iyermnqsls llynvpavae eiqdevdell qkeqnysddv lanmisepri sygndalmps 541 ltetkttvel lpvngefsld dlqpwhsfga dsvpantene vepvdarpaa drglttrpgs 601 gltnikteei sevkmdaefr hdsgyevhhq klvffaedvg snkgaiiglm vggvviatvi 661 vitlvmlkkk qytsihhgvv evdaavtpee rhlskmqqng yenptykffe qmqn

[0146] Protein sequence isoform c (SEQ ID NO: 24): 1 mlpglallll aawtaralev ptdgnaglalla epqiamfcgr lnmhmnvqng kwdsdpsgtk 61 tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg 121 efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr 181 gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee 241 eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrvp ttaastpdav 301 dkyletpgde nehahfqkak erleakhrer msqvmrewee aerqaknlpk adkkaviqhf 361 qekvesleqe aanerqqlve thmarveaml ndrrrlalen yitalqavpp rprhvfnmlk 421 kyvraeqkdr qhtlkhfehv rmvdpkkaaq irsqvmthlr viyermnqsl sllynvpava 481 eeiqdevdel lqkeqnysdd vlanmisepr isygndalmp sltetkttve llpvngefsl 541 ddlqpwhsfg adsvpanten evepvdarpa adrglttrpg sgltniktee isevkmdaef 601 rhdsgyevhh qklvffaedv gsnkgaiigl mvggvviatv ivitlvmlkk kqytsihhgv 661 vevdaavtpe erhlskmqqn gyenptykff eqmqn

[0147] Protein sequence isoform e (SEQ ID NO: 25): 1 mlpglallll aawtaralev ypelqitnvv eanqpvtiqn wckrgrkqck thphfvipyr 61 clvgefvsda llvpdkckfl hqermdvcet hlhwhtvake tcsekstnlh dygmllpcgi 121 dkfrgvefvc cplaeesdnv dsadaeedds dvwwggadtd yadgsedkvv evaeeeeevae 181 veeeeaddde ddedgdevee eaeepyeeat erttsiattt ttttesveev vrvpttaast 241 pdavdkylet pgdenehahf qkakerleak hrermsqvmr eweeaerqak nlpkadkkav 301 iqhfqekves leqeaanerq qlvethmarv eamlndrrrl alenyitalq avpprprhvf 361 nmlkkyvrae qkdrqhtlkh fehvrmvdpk kaaqirsqvm thlrviyerm nqslsllynv 421 pavaeeiqde vdellqkeqn ysddvlanmi seprisygnd almpsltetk ttvellpvng 481 efslddlqpw hsfgadsvpa ntenevepvd arpaadrglt trpgsgltni kteeisevkm 541 daefrhdsgy evhhqklvff aedvgsnkga iiglmvggvv iatvivitlv mlkkkqytsi 601 hhgvvevdaa vtpeerhlsk mqqngyenpt ykffeqmqn

[0148] Gene ID: X14 Gene symbol: COLEC12 Gene description: Collectin subfamily member 12 Unigene: Hs.464422 Genbank: AB038518 Entrez Gene: 81035 Refseq: NM_130386 Protein sequence (SEQ ID NO: 26): 1 mkddfaeeee vqsfgykrfg iqegtqctkc knnwalkfsi illyilcall titvailgyk 61 vvekmdnvtg gmetsrqtyd dkltavesdl kklgdqtgkk aistnselst frsdildlrq 121 qlreitekts knkdtleklq asgdalvdrq sqlketlenn sflittvnkt lqayngyvtn 181 lqqdtsvlqg nlqnqmyshn vvimnlnnln ltqvqqrnli tnlqrsvddt sqaiqriknd 241 fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql nsftgqmeni 301 ttisqaneqn lkdlqdlhkd aenrtaikfn qleerfqlfe tdivniisni sytahhlrtl 361 tsnlnevrtt ctdtltkhtd dltslnntla nirldsvslr mqqdlmrsrl dtevanlsvi 421 meemklvdsk hgqliknfti lqgppgprgp rgdrgsqgpp gptgnkgqkg ekgepgppgp 481 agergpigpa gppgerggkg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkeglpgp 541 qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgavv plqneptp 601 apedngcpph wknftdkcyy fsvekeifed aklfcedkss hlvfintree qqwikkqmvg 661 reshwigltd serenewkwl dgtspdyknw kagqpdnwgh ghgpgedcag liyagqwndf 721 qcedvnnfic ekdretvlss al

[0149] Gene ID: X15 Gene symbol: NCAM1 Gene description: neural cell adhesion molecule 1 Unigene: Hs.503878 Genbank: BC047244 Entrez Gene: 4684 Refseq: NM_000615|NM_001076682| NM_181351| NM_001242608| NM_001242607 Protein Sequence Isoform 1 (SEQ ID NO: 27): 1 mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge 61 kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf 121 knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik 181 ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf 241 peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati 301 hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv 361 vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn 421 qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct 481 avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav 541 geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvqg 601 epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei rlpsgsdhvm 661 lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip angsptsgls tgaivgiliv 721 ifvlllvvvd itcyflnkcg lfmciavnlc gkagpgakgk dmeegkaafs kdeskepive 781 vrteeertpn hdggkhtepn ettpltepek gpveakpecq etetkpapae vktvpndatq 841 tkeneska

[0150] Protein Sequence Isoform 3 (SEQ ID NO: 28): 1 mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge 61 kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf 121 knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik 181 ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf 241 peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati 301 hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv 361 vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn 421 qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct 481 avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav 541 geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvhs 601 ppppasasss tpvplsppdt twplpalatt epakgepsap klegqmgedg nsikvnlikq 661 ddggspirhy lvryralsse wkpeirlpsg sdhvmlksld wnaeyevyvv aenqqgkska 721 ahfvfrtsaq ptaipatlgg nsasytfvsl lfsavtllll c

[0151] Protein Sequence Isoform 2 (SEQ ID NO: 29): 1 mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge 61 kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf 121 knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik 181 ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf 241 peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati 301 hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eekaswtrpe 361 kqetldghmv vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg 421 pvavytwegn qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds 481 endfgnynct avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi 541 lkykaewrav geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa 601 sefktqpvqg epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei 661 rlpsgsdhvm lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip angsptsgls 721 tgaivgiliv ifvlllvvvd itcyflnkcg lfmciavnlc gkagpgakgk dmeegkaafs 781 kdeskepive vrteeertpn hdggkhtepn ettpltepek gpveakpecq etetkpapae 841 vktvpndatq tkeneska

[0152] Protein sequence Isoform 4 (SEQ ID NO: 30): 1 mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge 61 kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf 121 knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik 181 ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf 241 peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati 301 hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv 361 vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn 421 qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct 481 avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav 541 geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvqg 601 epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei rlpsgsdhvm 661 lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip atlggnsasy tfvsllfsav 721 tllllc

[0153] Protein Sequence Isoform 5 (SEQ ID NO: 31): 1 mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge 61 kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf 121 knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik 181 ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf 241 peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati 301 hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eekaswtrpe 361 kqevhapwnw qvgrqkgqag sagfpgshet ldghmvvrsh arvssltlks iqytdageyi 421 ctasntigqd sqsmylevqy apklqgpvav ytwegnqvni tcevfaypsa tiswfrdgql 481 lpssnysnik iyntpsasyl evtpdsendf gnynctavnr igqeslefil vqadtpssps 541 idqvepysst aqvqfdepea tggvpilkyk aewravgeev whskwydake asmegivtiv 601 glkpettyav rlaalngkgl geisaasefk tqpvqgepsa pklegqmged gnsikvnlik 661 qddggspirh ylvryralss ewkpeirlps gsdhvmlksl dwnaeyevyv vaenqqgksk 721 aahfvfrtsa qptaipangs ptsglstgai vgilivifvl llvvvditcy flnkcglfmc 781 iavnlcgkag pgakgkdmee gkaafskdes kepivevrte eertpnhdgg khtepnettp 841 ltepekgpve akpecqetet kpapaevktv pndatqtken eska

[0154] Gene ID: X16 Gene symbol: NRP2 Gene description: Neuropilin-2 Unigene: Hs.471200 Genbank: BX537423|AF016098|BC101525|BC104770|BC117413|BC143238|BC143608| AF022860|AF280545|AF280544|AF022859|AK290934|AF280546|BC009222| AL833606|BX648292|AK130198|BC018631 Entrez Gene: 8828 Refseq: NM_201266|NM_003872|NM_201279|NM_018534|NM_201267|NM_201264 Protein Sequence Isoform 1 (SEQ ID NO: 32): 1 mdmfpltwvf ​​lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy 61 apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml 121 yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil 181 akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs 241 stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys 301 dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks 361 yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial 421 rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq 481 aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd 541 prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt 601 vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd 661 hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy 721 qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr 781 sgeiaiddir istdvplenc mepisafage nfkvdipeih eregyedeid deyevdwsns 841 ssatsgsgap stdkekswly tldpilitii amsslgvllg atcaglllyc tcsysglssr 901 scttlenynf elydglkhkv kmnhqkccse a

[0155] Protein Sequence Isoform 2 (SEQ ID NO: 33): 1 mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy 61 apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml 121 yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil 181 akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs 241 stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys 301 dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks 361 yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial 421 rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq 481 aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd 541 prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt 601 vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd 661 hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy 721 qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr 781 sgeiaiddir istdvplenc mepisafavd ipeiheregy edeiddeyev dwsnsssats 841 gsgapstdke kswlytldpi litiiamssl gvllgatcag lllyctcsys glssrscttl 901 enynfelydg lkhkvkmnhq kccsea

[0156] Protein Sequence Isoform 3 (SEQ ID NO: 34): 1 mdmfpltwvf ​​lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy 61 apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml 121 yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil 181 akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs 241 stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys 301 dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks 361 yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial 421 rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq 481 aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd 541 prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt 601 vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd 661 hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy 721 qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr 781 sgeiaiddir istdvplenc mepisafade yevdwsnsss atsgsgapst dkekswlytl 841 dpilitiam sslgvllgat caglllyctc sysglssrsc ttlenynfel ydglkhkvkm 901 nhqkccsea

[0157] Protein Sequence Isoform 4 (SEQ ID NO: 35): 1 mdmfpltwvf ​​lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy 61 apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml 121 yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil 181 akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs 241 stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys 301 dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks 361 yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial 421 rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq 481 aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd 541 prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt 601 vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd 661 hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy 721 qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr 781 sgeiaiddir istdvplenc mepisafage nfkggtllpg teptvdtvpm qpipaywyyv 841 maaggavlvl vsvalalvlh yhrfryaakk tdhsitykts hytngaplav eptltikleq 901 drgshc

[0158] Protein Sequence Isoform 5 (SEQ ID NO: 36): 1 mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy 61 apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml 121 yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil 181 akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs 241 stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys 301 dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks 361 yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial 421 rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq 481 aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd 541 prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt 601 vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd 661 hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy 721 qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr 781 sgeiaiddir istdvplenc mepisafagg tllpgteptv dtvpmqpipa ywyyvmaagg 841 avlvlvsval alvlhyhrfr yaakktdhsi tyktshytng aplaveptlt ikleqdrgsh 901 c

[0159] Protein Sequence Isoform 6 (SEQ ID NO: 37): 1 mdmfpltwvf ​​lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy 61 apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml 121 yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil 181 akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs 241 stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys 301 dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks 361 yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial 421 rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq 481 aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd 541 prtqqpkvgc swrpl

[0160] Gene ID: X17 Gene symbol: PLXNA2 Gene description: Plexin-A2 Unigene: Hs.497626 Genbank: BC132676 Entrez Gene: 5362 Refseq: NM_025179 Protein sequence (SEQ ID NO: 38): 1 meqrrpwpra levdsrsvvl lsvvwvllap paagmpqfst fhsenrdwtf nhltvhqgtg 61 avyvgainrv ykltgnltiq vahktgpeed nkscyppliv qpcsevltlt nnvnklliid 121 ysenrllacg slyqgvckll rlddlfilve pshkkehyls svnktgtmyg vivrsegedg 181 klfigtavdg kqdyfptlss rklprdpess amldyelhsd fvsslikips dtlalvshfd 241 ifyiygfasg gfvyfltvqp etpegvains agdlfytsri vrlckddpkf hsyvslpfgc 301 tragveyrll qaaylakpgd slaqafnits qddvlfaifs kgqkqyhhpp ddsalcafpi 361 rainlqiker lqscyqgegn lelnwllgkd vqctkapvpi ddnfcgldin qplggstpve 421 gltlyttsrd rmtsvasyvy ngysvvfvgt ksgklkkira dgpphggvqy emvsvlkdgs 481 pilrdmafsi dqrylyvmse rqvtrvpves ceqyttcgec lssgdphcgw calhnmcsrr 541 dkcqqawepn rfaasisqcv slavhpssis vsehsrllsl vvsdapdlsa giacafgnlt 601 evegqvsgsq vicispgpkd vpvipldqdw fglelqlrsk etgkifvste fkfyncsahq 661 lclscvnsaf rchwckyrnl cthdpttcsf qegrinised cpqlvpteei lipvgevkpi 721 tlkarnlpqp qsgqrgyecv lniqgaihrv palrfnsssv qcqnssyqyd gmdisnlavd 781 favvwngnfi idnpqdlkvh lykcaaqres cglclkadrk fecgwcsger rctlhqhcts 841 psspwldwss hnvkcsnpqi teiltvsgpp eggtrvtihg vnlgldfsei ahhvqvagvp 901 ctplpgeyii aeqivcemgh alvgttsgpv rlcigeckpe fmtkshqqyt fvnpsvlsln 961 pirgpesggt mvtitghylg agssvavylg nqtcefygrs mseivcvspp ssnglgpvpv 1021 svsvdrahvd snlqfeyidd prvqriepew siasghtplt itgfnldviq eprirvkfng 1081 kesvnvckvv ntttltclap slttdyrpgl dtverpdefg fvfnnvqsll iyndtkfiyy 1141 pnptfellsp tgvldqkpgs piilkgknlc ppasggakln ytvligetpc avtvsetqll 1201 ceppnltgqh kvmvhvggmv fspgsvsvis dslltlpaiv siaaggslll iiviivliay 1261 krksrendlt lkrlqmqmdn lesrvalecheck eafaelqtdi neltsdldrs gipyldyrty 1321 amrvlfpgie dhpvlrelev qgngqqhvek alklfaqlin nkvflltfir tlelqrsfsm 1381 rdrgnvasli mtglqgrley atdvlkqlls dlidknlenk nhpklllrrt esvaekmltn 1441 wfafllhkfl kecageplfm lycaikqqme kgpidaitge aryslsedkl irqqieyktl 1501 ilncvnpdne nspeipvkvl ncdtitqvke kildavyknv pysqrpravd mdlewrqgri 1561 arvvlqdedi ttkiegdwkr lntlmhyqvs drsvvalpk qtssynipas asisrtsisr 1621 ydssfrytgs pdslrsrapm itpdlesgvk vwhlvknhdh gdqkegdrgs kmvseiyltr 1681 llatkgtlqk fvddlfetlf stvhrgsalp laikmfdfl deqadrhsih dtdvrhtwks 1741 nclplrfwvn viknpqfvfd ihkgsitdac lsvvaqtfmd scstsehrlg kdspsnklly 1801 akdipsyksw veryyadiak lpaisdqdmn aylaeqsrlh avefnmlsal neiysyvsky 1861 seeligaleq deqarrqrla ykveqlinam sies

[0161] Gene ID: X18 Gene symbol: PCDHA4 Gene description: Protocadherin alpha-4 Unigene: Hs.199343 Genbank: AF152482| AF152312 Entrez Gene: 56144 Refseq: NM_018907| NM_031500 Protein Sequence Isoform 1 (SEQ ID NO: 39): 1 mefswgsgqe srrllllll laaweagngq lhysvseeak hgtfvgriaq dlglelaelv 61 prlfrvaskg rggllevnlq ngilfvnsri dreelcrrsa ecsihleviv drplqvfhvd 121 vevrdindnp pvfpatqknl siaesrplds rfplegasda digenallty rlspneyfsl 181 ekppddelvk glglilrksl dreeapeifl vltatdggkp eltgtvqlli tvldandnap 241 afdrtiykvr llenvpngtl viklnasdld eglngdivys fsndispnvk skfhidpitg 301 qiivkgyidf eesksyeiiv egidkgqlpl sghcrvivev ednndnvpdl efkslslpir 361 edaplgtvia lisvsdkdmg vnglvtcslt shvpfklvst fknyyslvld saldresvsa 421 yelvvtardg gspslwatas vsvevadvnd napafaqpey tvfvkennpp gchiftvsaw 481 dadaqenalv syslverrvg eralssyvsv haesgkvyal qpldheelel lqfqvtarda 541 gvpplgsnvt lqvfvldend napallapra ggtggavsel vpwsvgvghv vakvravdad 601 sgynawlsye lqpgtggari pfrvglytge isttraldet daprhrllvl vkdhgepalt 661 atatvlvslv esgqapkass ralvgavgpd aalvdvnvyl iiaicavssl lvltlllyta 721 lrcsalpteg acapgkptlv cssavgswsy sqqrrprvcs gegppktdlm afspslpdsr 781 dredqlqtte esfakprqpn pdwrysaslr agmhssvhle eagilragpg gpdqqwptvs 841 satpepeage vsppvgagvn snswtfkygp gnpkqsgpge lpdkfiipgs paiisirqep 901 tnsqidksdf itfgkkeetk kkkkkkkgnk tqekkekgns ttdnsdq

[0162] Protein Sequence Isoform 2 (SEQ ID NO: 40): 1 mefswgsgqe srrllllll laaweagngq lhysvseeak hgtfvgriaq dlglelaelv 61 prlfrvaskg rggllevnlq ngilfvnsri dreelcrrsa ecsihleviv drplqvfhvd 121 vevrdindnp pvfpatqknl siaesrplds rfplegasda digenallty rlspneyfsl 181 ekppddelvk glglilrksl dreeapeifl vltatdggkp eltgtvqlli tvldandnap 241 afdrtiykvr llenvpngtl viklnasdld eglngdivys fsndispnvk skfhidpitg 301 qiivkgyidf eesksyeiiv egidkgqlpl sghcrvivev ednndnvpdl efkslslpir 361 edaplgtvia lisvsdkdmg vnglvtcslt shvpfklvst fknyyslvld saldresvsa 421 yelvvtardg gspslwatas vsvevadvnd napafaqpey tvfvkennpp gchiftvsaw 481 dadaqenalv syslverrvg eralssyvsv haesgkvyal qpldheelel lqfqvtarda 541 gvpplgsnvt lqvfvldend napallapra ggtggavsel vpwsvgvghv vakvravdad 601 sgynawlsye lqpgtggari pfrvglytge isttraldet daprhrllvl vkdhgepalt 661 atatvlvslv esgqapkass ralvgavgpd aalvdvnvyl iiaicavssl lvltlllyta 721 lrcsalpteg acapgkptlv cssavgswsy sqqrrprvcs gegppktdlm afspslpdsr 781 dredqlqtte esfakvsv

[0163] Gene ID: X19 Gene symbol: PCDHAC2 Gene description: Protocadherin alpha-C2 Unigene: Hs.199343 Genbank: AF152304 Entrez Gene: 56134 Refseq: NM_018899| NM_031883 Protein Sequence Isoform 1 (SEQ ID NO: 41): 1 meqagtrpaa tehprlrrpm pwllllplll llllllpgpa asqlrysvpe eqapgalvgn 61 varalglellr rlgpgclrin hlgapspryl eldltsgalf vneridreal ceqrprclls 121 levlahnpva vsaveveild indnsprfpr pnyqlqvses vapgarfhie saqdpdvgan 181 svqtyelsps ehfeldlkpl qenskvlelv lrkgldreqa alhhlvltav dggiparsgt 241 aqisvrvldt ndnspafdqs tyrvqlreds ppgtlvvkln asdpdegsng elryslssyt 301 sdrerqlfsi dastgevrvi ggldyeeass yqiyvqatdr gpvpmaghck vlvdivdvnd 361 napevvltdl yspvpenatp ntivavlsvn dqdsgpnrkv slgleatlpf rlngfgnsyt 421 lvvsgpldre rvavynitvt atdggipqlt slrtlkveis dindnppsfl edsysiyiqe 481 nnlpgvllct vqatdpdeke naevtyslle reiqglpvts yvsinsasgs lyavnsfdye 541 kfreffvtve aqdkgsppls stvtanvyvv dmndhaphil yptstnssaa femvprtapa 601 gylvtkviam dsdsgqnawl fyhlaqtsdl dlfkvelhtg eirttrkmgd esgstfnltv 661 vvrdngepsl sasvaitvav vdrvskilpd tqrhvksprt yseitlylii alstvsfifl 721 ltiilsiik cyrytaygta ccggfcgvre rspaelykqa nnnidariph glkvqphfie 781 vrgngsltkt ycykacltag sgsdtfmfyn tgaqtgpgps gaqaavtdsr nltgqsgqna 841 gnliilknea vsqneprqpn pdwrysaslr agmhssvhle eagilragpg gpdqqwptvs 901 satpepeage vsppvgagvn snswtfkygp gnpkqsgpge lpdkfiipgs paiisirqep 961 tnsqidksdf itfgkkeetk kkkkkkkgnk tqekkekgns ttdnsdq

[0164] Protein Sequence Isoform 2 (SEQ ID NO: 42): 1 meqagtrpaa tehprlrrpm pwllllplll llllllpgpa asqlrysvpe eqapgalvgn 61 varalglellr rlgpgclrin hlgapspryl eldltsgalf vneridreal ceqrprclls 121 levlahnpva vsaveveild indnsprfpr pnyqlqvses vapgarfhie saqdpdvgan 181 svqtyelsps ehfeldlkpl qenskvlelv lrkgldreqa alhhlvltav dggiparsgt 241 aqisvrvldt ndnspafdqs tyrvqlreds ppgtlvvkln asdpdegsng elryslssyt 301 sdrerqlfsi dastgevrvi ggldyeeass yqiyvqatdr gpvpmaghck vlvdivdvnd 361 napevvltdl yspvpenatp ntivavlsvn dqdsgpnrkv slgleatlpf rlngfgnsyt 421 lvvsgpldre rvavynitvt atdggipqlt slrtlkveis dindnppsfl edsysiyiqe 481 nnlpgvllct vqatdpdeke naevtyslle reiqglpvts yvsinsasgs lyavnsfdye 541 kfreffvtve aqdkgsppls stvtanvyvv dmndhaphil yptstnssaa femvprtapa 601 gylvtkviam dsdsgqnawl fyhlaqtsdl dlfkvelhtg eirttrkmgd esgstfnltv 661 vvrdngepsl sasvaitvav vdrvskilpd tqrhvksprt yseitlylii alstvsfifl 721 ltiilsiik cyrytaygta ccggfcgvre rspaelykqa nnnidariph glkvqphfie 781 vrgngsltkt ycykacltag sgsdtfmfyn tgaqtgpgps gaqaavtdsr nltgqsgqna 841 gnliilknea vsqnevrqws ggllqthafv thppiscdla llsh

[0165] Gene ID: X20 Gene symbol: GPC4 Gene description: Glypican 4 Unigene: Hs.58367 Genbank: AF030186 Entrez Gene: 2239 Refseq: NM_001448 Protein sequence (SEQ ID NO: 43): 1 mrllwklvil lplinssagd gllsrpiftq ephdvifpld lsksevilnc aangypsphy 61 rwkqngtdid ftmsyhyrld ggslainsph tdqdigmyqc latnllgtil srkaklqfay 121 iedfetktrs tvsvregqgv vllcgppphf gdlsyawtfn dnplyvqedn rrfvsqetgn 181 lyiakvepsd vgnytcfitn keaqrsvqgp ptplvqrtdg vmgeyepkie vrfpetiqaa 241 kdssvklecf algnpvpdis wrrldgsplp gkvkysksqa ileipnfqqe degfyecias 301 nlrgrnlakg qlifyappew eqkiqnthls iydnllweck asgkpnpwyt wlkngerlnp 361 eeriqiengt liitmlnvsd sgvyqcaaen kyqiiyanae lrvlasapdf skspvkkksf 421 vqvggdivig ckpnafpraa iswkrgtetl rqskriflle dgslkiynit rsdagsytci 481 atnqfgtakn tgslivkert vitvppskmd vtvgesivlp cqvshdpsie vvfvwffngd 541 vidlkkgvah feriggesvg dlmirniqlh hsgkylctvq ttleslsava diivrgppgp 601 pedvqvedis sttsqlswra gpdnnspiqi ftiqtrtpfs vgwqavatvp eilngktyna 661 tvvglspwve yefrvvagns igigepseps ellrtkasvp vvapvnihgg ggsrselvit 721 wesipeelqn gegfgyiimf rpvgsttwsk ekvssvessr fvyrnesiip lspfevkvgv 781 ynneggsls tvtivysged epqlaprgts lqsfsaseme vswnaiawnr ntgrvlgyev 841 lywtddskes migkirvsgn vttknitglk antiyfasvr ayntagtgps sppvnvttkk 901 sppsqppani awkltnsklc lnwehvktme nesevlgyki lyrqnrqskt hiletnntsa 961 ellvpfeedy lieirtvsdg gdgssseeir ipkmsslssr giqflepsth flsivivifh 1021 cfaiqpli

[0166] Gene ID: X21 Gene symbol: CNTN6 Gene description: Contactin 6 Unigene: Hs.387300 Genbank: AB003592 Entrez Gene: 27255 Refseq: NM_014461 Protein sequence (SEQ ID NO: 44): 1 marfglpall ctlavlsaal laaelksksc sevrrlyvsk gfnkndaplh eingdhlkic 61 pqgstccsqe meekyslqsk ddfksvvseq cnhlqavfas rykkfdeffk ellenaeksl 121 ndmfvktygh lymqnselfk dlfvelkryy vvgnvnleem lndfwarlle rmfrlvnsqy 181 hftdeylecv skyteqlkpf gdvprklklq vtrafvaart faqglavagd vvskvsvvnp 241 taqcthallk miycshcrgl vtvkpcynyc snimrgclan qgdldfewnn fidamlmvae 301 rlegpfnies vmdpidvkis daimnmqdns vqvsqkvfqg cgppkplpag risrsisesa 361 fsarfrphhp eerpttaagt sldrlvtdvk eklkqakkfw sslpsnvcnd ermaagngne 421 ddcwngkgks rylfavtgng lanqgnnpev qvdtskpdil ilrqimalrv mtskmknayn 481 gndvdffdis dessgegsgs gceyqqcpse fdynatdhag ksanekadsa gvrpgaqayl 541 ltvfcilflv mqrewr

[0167] Gene ID: X22 Gene symbol: SLC9A7 Gene description: Solute carrier family 9 (sodium / hydrogen exchanger), member 7 Unigene: Hs.496057 Genbank: AF298591 Entrez Gene: 84679 Refseq: NM_001257291| NM_032591 Protein Sequence Isoform 1 (SEQ ID NO: 45): 1 mepgdaarpg sgratgappp rllllplllg wglrvaaaas asssgaaaed ssameelate 61 keaeeshrqd svslltfill ltltiltiwl fkhrrvrflh etglamiygl ivgvilrygt 121 patsgrdksl sctqedrafs tllvnvsgkf feytlkgeis pgkinsveqn dmlrkvtfdp 181 evffnillpp iifhagyslk krhffrnlgs ilayaflgta vscfiignlm ygvvklmkim 241 gqlsdkfyyt dclffgaiis atdpvtvlai fnelhadvdl yallfgesvl ndavaivlss 301 sivayqpagl nthafdaaaf fksvgiflgi fsgsftmgav tgvvtalvtk ftklhcfpll 361 etalfflmsw stfllaeacg ftgvvavlfc gitqahytyn nlsvesrsrt kqlfevlhfl 421 aenfifsymg lalftfqkhv fspifiigaf vaiflgraah iyplsfflnl grrhkigwnf 481 qhmmmfsglr gamafalair dtasyarqmm ftttllivff tvwiigggtt pmlswlnirv 541 gveepseedq nehhwqyfrv gvdpdqdppp nndsfqvlqg dgpdsargnr tkqesawifr 601 lwysfdhnyl kpilthsgpp ltttlpawcg llarcltspq vydnqeplre edsdfilteg 661 dltltygdst vtangssssh tastslegsr rtkssseevl erdlgmgdqk vssrgtrlvf 721 pledna

[0168] Protein Sequence Isoform 2 (SEQ ID NO: 46): 1 mepgdaarpg sgratgappp rllllplllg wglrvaaaas asssgaaaed ssameelate 61 keaeeshrqd svslltfill ltltiltiwl fkhrrvrflh etglamiygl ivgvilrygt 121 patsgrdksl sctqedrafs tllvnvsgkf feytlkgeis pgkinsveqn dmlrkvtfdp 181 evffnillpp iifhagyslk krhffrnlgs ilayaflgta vscfiignlm ygvvklmkim 241 gqlsdkfyyt dclffgaiis atdpvtvlai fnelhadvdl yallfgesvl ndavaivlss 301 sivayqpagl nthafdaaaf fksvgiflgi fsgsftmgav tgvnanvtkf tklhcfplle 361 talfflmsws tfllaeaacgf tgvvavlfcg itqahytynn lsvesrsrtk qlfevlhfla 421 enfifsymgl alftfqkhvf spifiigafv aiflgraahi yplsfflnlg rrhkigwnfq 481 hmmmfsglrg amafalaird tasyarqmmf tttllivfft vwiigggttp mlswlnirvg 541 veepseedqn ehhwqyfrvg vdpdqdpppn ndsfqvlqgd gpdsargnrt kqesawifrl 601 wysfdhnylk pilthsgppl tttlpawcgl larcltspqv ydnqeplree dsdfiltegd 661 ltltygdstv tangssssht astslegsrr tkssseevle rdlgmgdqkv ssrgtrlvfp 721 ledna

[0169] Gene ID: X23 Gene symbol: PVRL3 Gene description: Poliovirus receptor-related 3 Unigene: Hs.293917 Genbank: AK075105 Entrez Gene: 25945 Refseq: NM_015480| NM_001243286| NM_001243288 Protein Sequence Isoform 1 (SEQ ID NO: 47): 1 martlrpspl cpgggkaqls sasllgagll lqpptpppll lllfplllfs rlcgalagpi 61 ivephvtavw gknvslkcli evnetitqis wekihgkssq tvavhhpqyg fsvqgeyqgr 121 vlfknyslnd atitlhnigf sdsgkyicka vtfplgnaqs sttvtvlvep tvslikgpds 181 lidggnetva aiciaatgkp vahidwegdl gemestttsf pnetatiisq yklfptrfar 241 grritcvvkh palekdirys fildiqyape vsvtgydgnw fvgrkgvnlk cnadanpppf 301 ksvwsrldgq wpdgllasdn tlhfvhpltf nysgvyickv tnslgqrsdq kviyisdppt 361 tttlqptiqw hpstadiedl atepkklpfp lstlatikdd tiatiiasvv ggalfivlvs 421 vlagifcyrr rrtfrgdyfa knyippsdmq kesqidvlqq deldsypdsv kkenknpvnn 481 lirkdyleep ektqwnnven lnrferpmdy yedlkmgmkf vsdehydene ddlvshvdgs 541 visrrewyv

[0170] Protein Sequence Isoform 2 (SEQ ID NO: 48): 1 martlrpspl cpgggkaqls sasllgagll lqpptpppll lllfplllfs rlcgalagpi 61 ivephvtavw gknvslkcli evnetitqis wekihgkssq tvavhhpqyg fsvqgeyqgr 121 vlfknyslnd atitlhnigf sdsgkyicka vtfplgnaqs sttvtvlvep tvslikgpds 181 lidggnetva aiciaatgkp vahidwegdl gemestttsf pnetatiisq yklfptrfar 241 grritcvvkh palekdirys fildiqyape vsvtgydgnw fvgrkgvnlk cnadanpppf 301 ksvwsrldgq wpdgllasdn tlhfvhpltf nysgvyickv tnslgqrsdq kviyisayns 361 vaslnc

[0171] Protein Sequence Isoform 3 (SEQ ID NO: 49): 1 maegwrwcfv rrtpgllrgp llprsfsgnp ralagpiive phvtavwgkn vslkclievn 61 etitqiswek ihgkssqtva vhhpqygfsv qgeyqgrvlf knyslndati tlhnigfsds 121 gkyickavtf plgnaqsstt vtvlveptvs likgpdslid ggnetvaaic iaatgkpvah 181 idwegdlgem estttsfpne tatiisqykl fptrfargrr itcvvkhpal ekdirysfil 241 diqyapevsv tgydgnwfvg rkgvnlkcna danpppfksv wsrldgqwpd gllasdntlh 301 fvhpltfnys gvyickvtns lgqrsdqkvi yisdvpfkqt ssiavagavi gavlalfiia 361 ifvtvlltpr kkrpsyldkv idlppthkpp plyeersppl pqkdlfqpeh lplqtqfker 421 evgnlqhsng lnsrsfdyed enpvgedgiq qmyplynqmc yqdrspgkhh qnndpkrvyi 481 dprehyv

[0172] Gene ID: X24 Gene symbol: SLC4A4 Gene description: Solute carrier family 4, sodium bicarbonate symporter, member 4 Unigene: Hs.5462 Genbank: AF011390 Entrez Gene: 8671 Refseq: NM_001098484|NM_001134742|NM_003759 Protein Sequence Isoform 1 (SEQ ID NO: 50): 1 medeavldrg asflkhvcde eeveghhtiy igvhvpksyr rrrhkrktg hkekkekeri 61 senysdksdi enadessssi lkplispaae rirfilgeed dspappqlft eldellavdg 121 qemewketar wikfeekveq ggerwskphv atlslhslfe lrtcmekgsi mldreasslp 181 qlvemivdhq ietgllkpel kdkvtytllr khrhqtkksn lrsladigkt vssasrmftn 241 pdngspamth rnltssslnd isdkpekdql knkfmkklpr daeasnvlvg evdfldtpfi 301 afvrlqqavm lgaltevpvp trflfillgp kgkaksyhei griatlmsd evfhdiayka 361 kdrhdliagi defldevil ppgewdpair ieppkslpss dkrknmysgg envqmngdtp 421 hdgghggggh gdceelqrtg rfcgglikdi krkapffasd fydalniqal sailfiylat 481 vtnaitfggl lgdatdnmqg vlesflgtav sgaifclfag qpltilsstg pvlvferllf 541 nfskdnnfdy lefrlwiglw saflclilva tdasflvqyf trfteegfss lisfifiyda 601 fkkmiklady ypinsnfkvg yntlfsctcv ppdpanisis ndttlapeyl ptmsstdmyh 661 nttfdwafls kkecskyggn lvgnncnfvp ditlmsfilf lgtytssmal kkfktspyfp 721 ttarklisdf aiilsilifc vidalvgvdt pklivpsefk ptspnrgwfv ppfgenpwwv 781 claaaipall vtilifmdqq itavivnrke hklkkgagyh ldlfwvailm vicslmalpw 841 yvaatvisia hidslkmete tsapgeqpkf lgvreqrvtg tlvfiltgls vfmapilkfi 901 pmpvlygvfl ymgvaslngv qfmdrlkllll mplkhqpdfi ylrhvplrrv hlftflqvlc 961 lallwilkst vaaiifpvmi lalvavrkgm dylfsqhdls flddvipekd kkkkedekkk 1021 kkkkgsldsd nddsdcpyse kvpsikipmd imeqqpflsd skpsdrersp tflerhtsc

[0173] Protein Sequence Isoform 3 (SEQ ID NO: 51): 1 medeavldrg asflkhvcde eeveghhtiy igvhvpksyr rrrhkrktg hkekkekeri 61 senysdksdi enadessssi lkplispaae rirfilgeed dspappqlft eldellavdg 121 qemewketar wikfeekveq ggerwskphv atlslhslfe lrtcmekgsi mldreasslp 181 qlvemivdhq ietgllkpel kdkvtytllr khrhqtkksn lrsladigkt vssasrmftn 241 pdngspamth rnltssslnd isdkpekdql knkfmkklpr daeasnvlvg evdfldtpfi 301 afvrlqqavm lgaltevpvp trflfillgp kgkaksyhei graiatlmsd evfhdiayka 361 kdrhdliagi defldevivl ppgewdpair ieppkslpss dkrknmysgg envqmngdtp 421 hdgghggggh gdceelqrtg rfcgglikdi krkapffasd fydalniqal sailfiylat 481 vtnaitfggl lgdatdnmqg vlesflgtav sgaifclfag qpltilsstg pvlvferllf 541 nfskdnnfdy lefrlwiglw saflclilva tdasflvqyf trfteegfss lisfifiyda 601 fkkmiklady ypinsnfkvg yntlfsctcv ppdpanisis ndttlapeyl ptmsstdmyh 661 nttfdwafls kkecskyggn lvgnncnfvp ditlmsfilf lgtytssmal kkfktspyfp 721 ttarklisdf aiilsilifc vidalvgvdt pklivpsefk ptspnrgwfv ppfgenpwwv 781 claaaipall vtilifmdqq itavivnrke hklkkgagyh ldlfwvailm vicslmalpw 841 yvaatvisia hidslkmete tsapgeqpkf lgvreqrvtg tlvfiltgls vfmapilkfi 901 pmpvlygvfl ymgvaslngv qfmdrlklll mplkhqpdfi ylrhvplrrv hlftflqvlc 961 lallwilkst vaaiifpvmi lalvavrkgm dylfsqhdls flddvipekd kkkkedekkk 1021 kkkkgsldsd nddekdhqhs lnathhadki pflqslgmps pprtpvkvvp qirieleped 1081 ndyfwrskgt ettl

[0174] Protein sequence isoform 2 (SEQ ID NO: 52): 1 mstenvegkp snlgergarr sstflrvvqp mfnhsiftsa vspaaerirf ilgeeddspa 61 ppqlftelde llavdgqeme wketarwikf eekveqgger wskphvatls lhslfelrtc 121 mekgsimldr easslpqlve mivdhqietg llkpelkdkv tytllrkhrh qtkksnlrsl 181 adigktvssa srmftnpdng spamthrnlt ssslndisdk pekdqlknkf mkklprdaea 241 snvlvgevdf ldtpfiafvr lqqavmlgal tevpvptrfl fillgpkgka ksyheigrai 301 atlmsdevfh diaykakdrh dliagidefl devivlppge wdpairiepp kslpssdkrk 361 nmysggenvq mngdtphdgg hggggghgdce elqrtgrfcg glikdikrka pffasdfyda 421 lniqalsail fiylatvtna itfggllgda tdnmqgvles flgtavsgai fclfagqplt 481 ilsstgpvlv ferllfnfsk dnnfdylefr lwiglwsafl clilvatdas flvqyftrft 541 eegfsslisf ifiydafkkm ikladyypin snfkvgyntl fsctcvppdp anisisndtt 601 lapeylptms stdmyhnttf dwaflskkec skyggnlvgn ncnfvpditl msfilflgty 661 tssmalkkfk tspyfpttar klisdfaiil silifcvida lvgvdtpkli vpsefkptsp 721 nrgwfvppfg enpwwvclaa aipallvtil ifmdqqitav ivnrkehklk kgagyhldlf 781 wvailmvics lmalpwyvaa tvisiahids lkmetetsap geqpkflgvr eqrvtgtlvf 841 iltglsvfma pilkfipmpv lygvflymgv aslngvqfmd rlklllmplk hqpdfiylrh 901 vplrrvhlft flqvlclall wilkstvaai ifpvmilalv avrkgmdylf sqhdlsfldd 961 vipekdkkkk edekkkkkkk gsldsdndds dcpysekvps ikipmdimeq qpflsdskps 1021 drersptfle rhtsc

[0175] Gene ID: X25 Gene symbol: CXADR Gene Description: Coxsackievirus and Adenovirus Receptors Unigene: Hs.634837 Genbank: AY072912| AY072911| AY072910| AK313526| Entrez Gene: 1525 Refseq: NM_001338|NM_001207063|NM_001207064|NM_001207065| NM_001207066 Protein Sequence Isoform 1 (SEQ ID NO: 53): 1 mallllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis 61 padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc 121 kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd 181 sqkmptswla emtssvisvk nasseysgty sctvrnrvgs dqcllrlnvv ppsnkaglia 241 gaiigtllal aligliifcc rkkrreekye kevhhdired vpppksrtst arsyigsnhs 301 slgsmspsnm egysktqynq vpsedfertp qsptlppakv aapnlsrmga ipvmipaqsk 361 dgsiv

[0176] Protein Sequence Isoform 2 (SEQ ID NO: 54): 1 mallllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis 61 padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc 121 kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd 181 sqkmptswla gkmchlqrav rplpeatsav iihpwgpcll ptwkdiprls itkyqvktln 241 allrvrlshl lr

[0177] Protein Sequence Isoform 3 (SEQ ID NO: 55): 1 mallllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis 61 padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc 121 kvkkapgvan kkihlvvlgk mchlqravrp lpeatsavii hpwgpcllpt wkdiprlsit 181 kyqvktlnal lrvrlshllr

[0178] Protein Sequence Isoform 4 (SEQ ID NO: 56): 1 mallllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis 61 padnqkvdqv grcatskepy vhcqklhrq

[0179] Protein Sequence Isoform 5 (SEQ ID NO: 57): 1 mallllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis 61 padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc 121 kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd 181 sqkmptswla emtssvisvk nasseysgty sctvrnrvgs dqcllrlnvv ppsnkaglia 241 gaiigtllal aligliifcc rkkrreekye kevhhdired vpppksrtst arsyigsnhs 301 slgsmspsnm egysktqynq vpsedfertp qsptlppakf kypyktdgit vv

[0180] Gene ID: X26 Gene symbol: CADM4 Gene description: Cell adhesion molecule 4 Unigene: Hs.370984 Genbank: AF363368 Entrez Gene: 199731 Refseq: NM_145296 Protein sequence (SEQ ID NO: 58): 1 mgrarrfqwp llllwaaaag pgagqevqte nvtvaeggva eitcrlhqyd gsivviqnpa 61 rqtlffngtr alkderfqle efsprrvrir lsdarledeg gyfcqlyted thhqiatltv 121 lvapenpvve vreqavegge velsclvprs rpaatlrwyr drkelkgvss sqengkvwsv 181 astvrfrvdr kddggiiiice aqnqalpsgh skqtqyvldv qysptariha sqavvregdt 241 lvltcavtgn prpnqirwnr gneslperae avgetltlpg lvsadngtyt ceasnkhgha 301 ralyvlvvyd pgavveaqts vpyaivggil allvfliicv lvgmvwcsvr qkgsylthea 361 sgldeqgear eaflngsdgh krkeeffi

[0181] Gene ID: Y1 Gene symbol: CLCA2 Gene description: Chloride channel accessory 2 Unigene: Hs.241551 Genbank: BC041096 Entrez Gene: 9635 Refseq: NM_006536 Protein sequence (SEQ ID NO: 59): 1 mtqrsiagpi cnlkfvtllv alsselpflg agvqlqdngy nglliainpq vpenqnlisn 61 ikemiteasf ylfnatkrrv ffrnikilip atwkannnsk ikqesyekan vivtdwygah 121 gddpytlqyr gcgkegkyih ftpnfllndn ltagygsrgr vfvhewahlr wgvfdeynnd 181 kpfyingqnq ikvtrcssdi tgifvcekgp cpqenciisk lfkegctfiy nstqnatasi 241 mfmqslssvv efcnasthnq eapnlqnqmc slrsawdvit dsadfhhsfp mngtelpppp 301 tfslvqagdk vvclvldvss kmaeadrllq lqqaaefylm qiveihtfvg iasfdskgei 361 raqlhqinsn ddrkllvsyl pttvsaktdi sicsglkkgf evveklngka ygsvmilvts 421 gddkllgncl ptvlssgsti hsialgssaa pnleelsrlt gglkffvpdi snsnsmidaf 481 srissgtgdi fqqhiqlest genvkphhql kntvtvdntv gndtmflvtw qasgppeiil 541 fdpdgrkyyt nnfitnltfr taslwipgta kpghwtytln nthhslqalk vtvtsrasns 601 avppatveaf verdslhfph pvmiyanvkq gfypilnatv tatvepetgd pvtlrllddg 661 agadvikndg iysryffsfa angryslkvh vnhspsistp ahsipgsham yvpgytangn 721 iqmnaprksv grneeerkwg fsrvssggsf svlgvpagph pdvfppckii dleavkveee 781 ltlswtapge dfdqgqatsy eirmskslqn iqddfnnail vntskrnpqq agireiftfs 841 pqistngpeh qpngethesh riyvairamd rnslqsavsn iaqaplfipp nsdpvpardy 901 lilkgvltam gligiiclii vvthhtlsrk kradkkengt kll

[0182] Gene ID: Y2 Gene symbol: ECM1 Gene description: extracellular matrix protein 1 Unigene: Hs.81071 Genbank: U68187| U68186| AK097046 Entrez Gene: 1893 Refseq: NM_004425|NM_022664| NM_001202858 Protein Sequence Isoform 1 (SEQ ID NO: 60): 1 mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp 61 dssqhgppfe gqsqvqppps qeatplqqek llpaqlpaek evgpplpqea vplqkelpsl 121 qhpneqkegt papfgdqshp epeswnaaqh cqqdrsqggw ghrldgfppg rpspdnlnqi 181 clpnrqhvvy gpwnlpqssy shltrqgetl nfleigysrc chcrshtnrl ecaklvweea 241 msrfceaefs vktrphwcct rqgearfscf qeeapqphyq lracpshqpd issglelpfp 301 pgvptldnik nichlrrfrs vprnlpatdp lqrellaliq lerefqrccr qgnnhtctwk 361 awedtldkyc dreyavkthh hlccrhppsp trdecfarra pypnydrdil tidigrvtpn 421 lmghlcgnqr vltkhkhipg lihnmtarcc dlpfpeqacc aeeekltfin dlcgprrniw 481 rdpalccyls pgdeqvncfn inylrnvalv sgdtenakgq geqgstggtn isstsepkee

[0183] Protein Sequence Isoform 2 (SEQ ID NO: 61): 1 mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp 61 dssqhgppfe gqsqvqppps qeatplqqek llpaqlpaek evgpplpqea vplqkelpsl 121 qhpneqkegt papfgdqshp epeswnaaqh cqqdrsqggw ghrldgfppg rpspdnlnqi 181 clpnrqhvvy gpwnlpqssy shltrqgetl nfleigysrc chcrshtnrl ecaklvwedt 241 ldkycdreya vkthhhlccr hppsptrdec farrapypny drdiltidig rvtpnlmghl 301 cgnqrvltkh khipglihnm tarccdlpfp eqaccaeeek ltfindlcgp rrniwrdpal 361 ccylspgdeq vncfninylr nvalvsgdte nakgqgeqgs tggtnissts epkee

[0184] Protein Sequence Isoform 3 (SEQ ID NO: 62): 1 mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp 61 dssqhgppfe gqsgkegrgp rphsqpwlge rvgcshipps ivqpppsqea tplqqekllp 121 aqlpaekevg pplpqeavpl qkelpslqhp neqkegtpap fgdqshpepe swnaaqhcqq 181 drsqggwghr ldgfppgrps pdnlnqiclp nrqhvvygpw nlpqssyshl trqgetlnfl 241 eigysrcchc rshtnrleca klvweeamsr fceaefsvkt rphwcctrqg earfscfqee 301 apqphyqlra cpshqpdiss grelpfppgv ptldniknic hlrrfrsvpr nlpatdplqr 361 ellaliqler efqrccrqgn nhtctwkawe dtldkycdre yavkthhhlc crhppsptrd 421 ecfarrapyp nydrdiltid igrvtpnlmg hlcgnqrvlt khkhipglih nmtarccdlp 481 fpeqaccaee ekltfindlc gprrniwrdp alccylspgd eqvncfniny lrnvalvsgd 541 tenakgqgeq gstggtniss tsepkee

[0185] Gene ID: Y3 Gene symbol: CLDN1 Gene description: claudin 1 Unigene: Hs.439060 Genbank: AY358652 Entrez Gene: 9076 Refseq: NM_021101 Protein sequence (SEQ ID NO: 63): 1 managlqllg filaflgwig aivstalpqw riysyagdni vtaqamyegl wmscvsqstg 61 qiqckvfdsl lnlsstlqat ralmvvgill gviaifvatv gmkcmkcled devqkmrmav 121 iggaifllag lailvatawy gnrivqefyd pmtpvnarye fgqalftgwa aaslcllgga 181 llccscprkt tsyptprpyp kpapssgkdy v

[0186] Gene ID: Y4 Gene symbol: SFN Gene description: stratifin Unigene: Hs.523718 Genbank: AF029082 Entrez Gene: 2810 Refseq: NM_006142 Protein sequence (SEQ ID NO: 64): 1 merasliqka klaeqaerye dmaafmkgav ekgeelscee rnllsvaykn vvggqraawr 61 vlssieqksn eegseekgpe vreyrekvet elqgvcdtvl glldshlike agdaesrvfy 121 lkmkgdyyry laevatgddk kriidsarsa yqeamdiskk empptnpirl gralnfsvfh 181 yeianspeea islakttfde amadlhtlse dsykdstlim qllrdnltlw tadnageegg 241 eapqepqs

[0187] Gene ID: Y5 Gene symbol: CD9 Gene description: CD9 antigen Unigene: Hs.114286 Genbank: AY966455 Entrez Gene: 928 Refseq: NM_001769 Protein sequence (SEQ ID NO: 65): 1 mpvkggtkci kyllfgfnfi fwlagiavla iglwlrfdsq tksifeqetn nnnssfytgv 61 yiligagalm mlvgflgccg avqesqcmlg lffgfllvif aieiaaaiwg yshkdevike 121 vqefykdtyn klktkdepqr etlkaihyal nccglaggve qfisdicpkk dvletftvks 181 cpdaikevfd nkfhiigavg igiavvmifg mifsmilcca irrnremv

[0188] Gene ID: Y6 Gene symbol: CD109 Gene description: CD109 antigen Unigene: Hs.399891 Genbank: AF410459 Entrez Gene: 135228 Refseq: NM_133493|NM_001159587|NM_001159588 Protein Sequence Isoform 1 (SEQ ID NO: 66): 1 mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka 61 ellktasnlt vsvleaegvf ekgsfktltl pslplnsade iyelrvtgrt qdeilfsnst 121 rlsfetkris vfiqtdkaly kpkqevkfri vtlfsdfkpy ktslnilikd pksnliqqwl 181 sqqsdlgvis ktfqlsshpi lgdwsiqvqv ndqtyyqsfq vseyvlpkfe vtlqtplycs 241 mnskhlngti takytygkpv kgdvtltflp lsfwgkkkni tktfkingsa nfsfndeemk 301 nvmdssngls eyldlsspgp veilttvtes vtgisrnvst nvffkqhdyi ieffdyttvl 361 kpslnftatv kvtradgnql tleerrnnvv itvtqrnyte ywsgsnsgnq kmeavqkiny 421 tvpqsgtfki efpiledsse lqlkayflgs kssmavhslf kspsktyiql ktrdenikvg 481 spfelvvsgn krlkelsymv vsrgqlvavg kqnstmfslt penswtpkac vivyyieddg 541 eiisdvlkip vqlvfknkik lywskvkaep sekvslrisv tqpdsivgiv avdksvnlmn 601 asnditmenv vhelelyntg yylgmfmnsf avfqecglwv ltdanltkdy idgvydnaey 661 aerfmeeneg hivdihdfsl gssphvrkhf petwiwldtn mgyriyqefe vtvpdsitsw 721 vatgfvised lglgltttpv elqafqpffi flnlpysvir geefaleiti fnylkdatev 781 kviieksdkf dilmtsnein atghqqtllv psedgatvlf pirpthlgei pitvtalspt 841 asdavtqmil vkaegieksy sqsilldltd nrlqstlktl sfsfppntvt gservqitai 901 gdvlgpsing laslirmpyg cgeqnminfa pniyildylt kkkqltdnlk ekalsfmrqg 961 yqrellyqre dgsfsafgny dpsgstwlsa fvlrcflead pyididqnvl hrtytwlkgh 1021 qksngefwdp grvihselqg gnkspvtlta yivtsllgyr kyqpnidvqe sihflesefs 1081 rgisdnytla lityalssvg spkakealnm ltwraeqegg mqfwvssesk lsdswqprsl 1141 dievaayall shflqfqtse gipimrwlsr qrnslggfas tqdttvalka lsefaalmnt 1201 ertniqvtvt gpsspspvkf lidthnrll qtaelavvqp tavnisangf gfaicqlnvv 1261 ynvkasgssr rrrsiqnqea fdldvavken kddlnhvdln vctsfsgpgr sgmalmevnl 1321 lsgfmvpsea islsetvkkv eydhgklnly ldsvnetqfc vnipavrnfk vsntqdasvs 1381 ivdyyeprrq avrsynsevk lsscdlcsdv qgcrpcedga sgshhhssvi fifcfkllyf 1441 melwl

[0189] Protein Sequence Isoform 2 (SEQ ID NO: 67): 1 mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka 61 ellktasnlt vsvleaegvf ekgsfktltl pslplnsade iyelrvtgrt qdeilfsnst 121 rlsfetkris vfiqtdkaly kpkqevkfri vtlfsdfkpy ktslnilikd pksnliqqwl 181 sqqsdlgvis ktfqlsshpi lgdwsiqvqv ndqtyyqsfq vseyvlpkfe vtlqtplycs 241 mnskhlngti takytygkpv kgdvtltflp lsfwgkkkni tktfkingsa nfsfndeemk 301 nvmdssngls eyldlsspgp veilttvtes vtgisrnvst nvffkqhdyi ieffdyttvl 361 kpslnftatv kvtradgnql tleerrnnvv itvtqrnyte ywsgsnsgnq kmeavqkiny 421 tvpqsgtfki efpiledsse lqlkayflgs kssmavhslf kspsktyiql ktrdenikvg 481 spfelvvsgn krlkelsymv vsrgqlvavg kqnstmfslt penswtpkac vivyyieddg 541 eiisdvlkip vqlvfknkik lywskvkaep sekvslrisv tqpdsivgiv avdksvnlmn 601 asnditmenv vhelelyntg yylgmfmnsf avfqecglwv ltdanltkdy idgvydnaey 661 aerfmeeneg hivdihdfsl gssphvrkhf petwiwldtn mgyriyqefe vtvpdsitsw 721 vatgfvised lglgltttpv elqafqpffi flnlpysvir geefaleiti fnylkdatev 781 kviieksdkf dilmtsnein atghqqtllv psedgatvlf pirpthlgei pitvtalspt 841 asdavtqmil vkaegieksy sqsilldltd nrlqstlktl sfsfppntvt gservqitai 901 gdvlgpsing laslirmpyg cgeqnminfa pniyildylt kkkqltdnlk ekalsfmrqg 961 yqrellyqre dgsfsafgny dpsgstwlsa fvlrcflead pyididqnvl hrtytwlkgh 1021 qksngefwdp grvihselqg gnkspvtlta yivtsllgyr kyqpnidvqe sihflesefs 1081 rgisdnytla lityalssvg spkakealnm ltwraeqegg mqfwvssesk lsdswqprsl 1141 dievaayall shflqfqtse gipimrwlsr qrnslggfas tqdttvalka lsefaalmnt 1201 ertniqvtvt gpsspsplav vqptavnisa ngfgfaicql nvvynvkasg ssrrrsiqn 1261 qeafdldvav kenkddlnhv dlnvctsfsg pgrsgmalme vnllsgfmvp seaislsetv 1321 kkveydhgkl nlyldsvnet qfcvnipavr nfkvsntqda svsivdyyep rrqavrsyns 1381 evklsscdlc sdvqgcrpce dgasgshhhs svififcfkl lyfmelwl

[0190] Protein Sequence Isoform 3 (SEQ ID NO: 68): 1 mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka 61 ellktasnlt vsvleaegvf ekgsfktltl psdpksnliq qwlsqqsdlg visktfqlss 121 hpilgdwsiq vqvndqtyyq sfqvseyvlp kfevtlqtpl ycsmnskhln gtitakytyg 181 kpvkgdvtlt flplsfwgkk knitktfkin gsanfsfnde emknvmdssn glseyldlss 241 pgpveilttv tesvtgisrn vstnvffkqh dyiieffdyt tvlkpslnft atvkvtradg 301 nqltleerrn nvvitvtqrn yteywsgsns gnqkmeavqk inytvpqsgt fkiefpiled 361 sselqlkayf lgskssmavh slfkspskty iqlktrdeni kvgspfelvv sgnkrlkels 421 ymvvsrgqlv avgkqnstmf sltpenswtp kacvivyyie ddgeiisdvl kipvqlvfkn 481 kiklywskvk aepsekvslr isvtqpdsiv givavdksvn lmnasnditm envvhelely 541 ntgyylgmfm nsfavfqecg lwvltdanlt kdyidgvydn aeyaerfmee neghivdihd 601 fslgssphvr khfpetwiwl dtnmgyriyq efevtvpdsi tswvatgfvi sedlglgltt 661 tpvelqafqp ffiflnlpys virgeefale itifnylkda tevkviieks dkfdilmtsn 721 einatghqqt llvpsedgat vlfpirpthl geipitvtal sptasdavtq milvkaegie 781 ksysqsild ltdnrlqstl ktlsfsfppn tvtgservqi taigdvlgps inglaslirm 841 pygcgeqnmi nfapniyild yltkkkqltd nlkekalsfm rqgyqrelly qredgsfsaf 901 gnydpsgstw lsafvlrcfl eadpyididq nvlhrtytwl kghqksngef wdpgrvihse 961 lqggnkspvt ltayivtsll gyrkyqpnid vqesihfles efsrgisdny tlalityals 1021 svgspkakea lnmltwraeq eggmqfwvss esklsdswqp rsldievaay allshflqfq 1081 tsegipimrw lsrqrnslgg fastqdttva lkalsefaal mntertniqv tvtgpsspsp 1141 vkflidthnr lllqtaelav vqptavnisa ngfgfaicql nvvynvkasg ssrrrsiqn 1201 qeafdldvav kenkddlnhv dlnvctsfsg pgrsgmalme vnllsgfmvp seaislsetv 1261 kkveydhgkl nlyldsvnet qfcvnipavr nfkvsntqda svsivdyyep rrqavrsyns 1321 evklsscdlc sdvqgcrpce dgasgshhhs svififcfkl lyfmelwl

[0191] Gene ID: Y7 Gene symbol: ITGB8 Gene description: integrin, beta8 Unigene: Hs.592171 Genbank: M73780 Entrez Gene: 3696 Refseq: NM_002214 Protein sequence (SEQ ID NO: 69): 1 mcgsalafft aafvclqndr rgpasflwaa wvfslvlglg qgednrcass naascarcla 61 lgpecgwcvq edfisggsrs ercdivsnli skgcsvdsie ypsvhviipt eneintqvtp 121 gevsiqlrpg aeanfmlkvh plkkypvdly ylvdvsasmh nnieklnsvg ndlsrkmaff 181 srdfrlgfgs yvdktvspyi sihperihnq csdynldcmp phgyihvlsl tenitefeka 241 vhrqkisgni dtpeggfdam lqaavceshi gwrkeakrll lvmtdqtshl aldsklagiv 301 vpndgnchlk nnvyvksttm ehpslgqlse klidnninvi favqgkqfhw ykdllpllpg 361 tiageieska anlnnlvvea yqklisevkv qvenqvqgiy fnitaicpdg srkpgmegcr 421 nvtsndevlf nvtvtmkkcd vtggknyaii kpigfnetak ihihrncscq cednrgpkgk 481 cvdetfldsk cfqcdenkch fdedqfsses cshkdqpvc sgrgvcvcgk cschkiklgk 541 vygkycekdd fscpyhhgnl caghgeceag rcqcfsgweg drcqcpsaaa qhcvnskgqv 601 csgrgtcvcg rcectdprsi grfcehcptc ytackenwnc mqclhphnls qaildqckts 661 calmeqqhyv dqtsecfssp sylriffiif ivtfligllk vliirqvilq wnsnkiksss 721 dyrvsaskkd klilqsvctr avtyrrekpe eikmdiskln ahetfrcnf

[0192] Gene ID: Y8 Gene symbol: EMP2 Gene description: epithelial membrane protein 2 Unigene: Hs.531561 Genbank: BC009687 Entrez Gene: 2013 Refseq: NM_001424 Protein sequence (SEQ ID NO: 70): 1 mlvllafiia fhitsaallf iatvdnawwv gdeffadvwr ictnntnctv indsfqeyst 61 lqavqatmil stilcciaff ifvlqlfrlk qgerfvltsi iqlmsclcvm iaasiytdrr 121 edihdknakf ypvtregsyg ysyilawvaf actfisgmmy lilrkrk

[0193] Gene ID: Y9 Gene symbol: FGFBP1 Gene description: fibroblast growth factor binding protein 1 Unigene: Hs.1690 Genbank: BC008910 Entrez Gene: 9982 Refseq: NM_005130 Protein sequence (SEQ ID NO: 71): 1 mkicsltlls flllaaqvll vegkkkvkng lhskvvseqk dtlgntqikq ksrpgnkgkf 61 vtkdqancrw aateqeegis lkvectqldh efscvfagnp tsclklkder vywkqvarnl 121 rsqkdicrys ktavktrvcr kdfpesslkl vsstlfgntk prkektemsp rehikgkett 181 psslavtqtm atkapecved pdmanqrkta lefcgetwss lctfflsivq dtsc

[0194] Gene ID: Y10 Gene symbol: CDH3 Gene description: cadherin 3, type 1, P-cadherin (placental) Unigene: Hs.191842 Genbank: BC041846 Entrez Gene: 1001 Refseq: NM_001793 Protein sequence (SEQ ID NO: 72): 1 mglprgplas llllqvcwlq caasepcrav freaevtlea ggaeqepgqa lgkvfmgcpg 61 qepalfstdn ddftvrnget vqerrslker npllkifpskr illrrhkrdwv vapisvpeng 121 kgpfpqrlnq lksnkdrdtk ifysitgpga dsppegvfav eketgwllln kpldreeiak 181 yelfghavse ngasvedpmn isiivtdqnd hkpkftqdtf rgsvlegvlp gtsvmqvtat 241 deddaiytyn gvvaysihsq epkdphdlmf tihrstgtis vissgldrek vpeytltiqa 301 tdmdgdgstt tavavveild andnapmfdp qkyeahvpen avghevqrlt vtdldapnsp 361 awratylimg gddgdhftit thpesnqgil ttrkgldfea knqhtlyvev tneapfvlkl 421 ptstativvh vedvneapvf vppskvvevq egiptgepvc vytaedpdke nqkisyrilr 481 dpagwlamdp dsgqvtavgt ldredeqfvr nniyevmvla mdngsppttg tgtllltlid 541 vndhgpvpep rqiticnqsp vrqvlnitdk dlsphtspfq aqltddsdiy wtaevneegd 601 tvvlslkkfl kqdtydvhls lsdhgnkeql tviratvcdc hghvetcpgp wkggfilpvl 661 gavlallfll lvllllvrkk rkikeplllp eddtrdnvfy ygeegggeed qdyditqlhr 721 glearpevvl rndvaptiip tpmyrprpan pdeignfiie nlkaantdpt appydtllvf 781 dyegsgsdaa slssltssas dqdqdydyln ewgsrfkkla dmygggedd

[0195] Gene ID: Y11 Gene symbol: ITGB4 Gene description: Integrin, beta 4 Unigene: Hs.632226 Genbank: X53587 Entrez Gene: 3691 Refseq: NM_000213|NM_001005619|NM_001005731 Protein Sequence Isoform 1 (SEQ ID NO: 73): 1 magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr 61 cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel 121 evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv 181 pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail 241 qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr 301 tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle 361 eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg 421 thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv 481 csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn 541 fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr 601 chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde 661 lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll 721 llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr 781 sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec 841 aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk 901 ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql 961 lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021 ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081 vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141 rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201 aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261 nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321 krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddtg cgwkfepllg 1381 eeldlrrvtw rlppeliprl sassgrssda eaphgppddg gaggkggslp rsatpgppge 1441 hlvngrmdfa fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh 1501 shsttlprdy stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy 1561 sveyqllngg elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities 1621 qvhpqsplcp lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem 1681 aqgggpataf rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg 1741 pfpqlgsrag lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef 1801 vsrtlttsgt lsthmdqqff qt

[0196] Protein Sequence Isoform 2 (SEQ ID NO: 74): 1 magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr 61 cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel 121 evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv 181 pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail 241 qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr 301 tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle 361 eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg 421 thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv 481 csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn 541 fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr 601 chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde 661 lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll 721 llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr 781 sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec 841 aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk 901 ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql 961 lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021 ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081 vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141 rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201 aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261 nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321 krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381 fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy 1441 stltsvsshg lppiwehgrs rlplswalgs rsraqmkgfp psrgprdsii lagrpaapsw 1501 gpdsrltagv pdtptrlvfs algptslrvs wqeprcerpl qgysveyqll nggelhrlni 1561 pnpaqtsvvv edllpnhsyv frvraqsqeg wgreregvit iesqvhpqsp lcplpgsaft 1621 lstpsapgpl vftalspdsl qlswerprrp ngdivgylvt cemaqgggpa tafrvdgdsp 1681 esrltvpgls envpykfkvq arttegfgpe regitiesq dggpfpqlgs raglfqhplq 1741 seyssittth tsatepflvd gltlgaqhle aggsltrhvt qefvsrtltt sgtlsthmdq 1801 qffqt

[0197] Protein Sequence Isoform 3 (SEQ ID NO: 75): 1 magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr 61 cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel 121 evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv 181 pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail 241 qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr 301 tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle 361 eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg 421 thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv 481 csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn 541 fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr 601 chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde 661 lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll 721 llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr 781 sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec 841 aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk 901 ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql 961 lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021 ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081 vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141 rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201 aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261 nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321 krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381 fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy 1441 stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy sveyqllngg 1501 elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities qvhpqsplcp 1561 lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem aqgggpataf 1621 rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg pfpqlgsrag 1681 lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef vsrtlttsgt 1741 lsthmdqqff qt

[0198] Gene ID: Y12 Gene symbol: LAMB3 Gene description: Laminin, beta 3 Unigene: Hs.497636 Genbank: BC075838 Entrez Gene: 3914 Refseq: NM_000228 Protein sequence (SEQ ID NO: 76): 1 mrpffllcfa lpgllhaqqa csrgacyppv gdllvgrtrf lrasstcglt kpetyctqyg 61 ewqmkcckcd srqphnyysh rvenvasssg pmrwwqsqnd vnpvslqldl drrfqlqevm 121 mefqgpmpag mlierssdfg ktwrvyqyla adctstfprv rqgrpqswqd vrcqslpqrp 181 narlnggkvq lnlmdlvsgi patqsqkiqe vgeitnlrvn ftrlapvpqr gyhppsayya 241 vsqlrlqgsc fchghadrca pkpgasagps tavqvhdvcv cqhntagpnc ercapfynnr 301 pwrpaegqda hecqrcdcng hsetchfdpa vfaasqgayg gvcdncrdht egkncercql 361 hyfrnrrpga siqetcisce cdpdgavpga pcdpvtgqcv ckehvqgerc dlckpgftgl 421 tyanpqgchr cdcnilgsrr dmpcdeesgr clclpnvvgp kcdqcapyhw klasgqgcep 481 cacdphnsls pqcnqftgqc pcregfgglm csaaairqcp drtygdvatg cracdcdfrg 541 tegpgcdkas grclcrpglt gprcdqcqrg ycnrypvcva chpcfqtyda dlreqalrfg 601 rlrnataslw sgpgledrgl asrildaksk ieqiravlss pavteqevaq vasailslrr 661 tlqglqldlp leeetlslpr dlesldrsfn glltmyqrkr eqfekissad psgafrmlst 721 ayeqsaqaaq qvsdssrlld qlrdsrreae rlvrqagggg gtgspklval rlemsslpdl 781 tptfnklcgn srqmactpis cpgelcpqdn gtacgsrcrg vlpraggafl magqvaeqlr 841 gfnaqlqrtr qmiraaeesa sqiqssaqrl etqvsasrsq meedvrrtrl liqqvrdflt 901 dpdtdaatiq evseavlalw lptdsatvlq kmneiqaiaa rlpnvdlvls qtkqdiarar 961 rlqaeaeear srahavegqv edvvgnlrqg tvalqeaqdt mqgtsrslrl iqdrvaevqq 1021 vlrpaeklvt smtkqlgdfw trmeelrhqa rqqgaeavqa qqlaegaseq alsaqegfer 1081 ikqkyaelkd rlgqssmlge qgariqsvkt eaeelfgetm emmdrmkdme lellrgsqai 1141 mlrsadltgl ekrveqirdh ingrvlyyat ck

[0199] Gene ID: Y13 Gene symbol: CD55 Gene description: CD55 antigen Unigene: Hs.126517 Genbank: M31516 Entrez Gene: 1604 Refseq: NM_000574 Protein sequence (SEQ ID NO: 77): 1 mtvarpsvpa alpllgelpr llllvllclp avwgdcglpp dvpnaqpale grtsfpedtv 61 itykceesfv kipgekdsvi clkgsqwsdi eefcnrscev ptrlnsaslk qpyitqnyfp 121 vgtvveyecr pgyrrepsls pkltclqnlk wstavefckk kscpnpgeir ngqidvpggi 181 lfgatisfsc ntgyklfgst ssfclisgss vqwsdplpec reiycpappq idngiiqger 241 dhygyrqsvt yacnkgftmi gehsiyctvn ndegewsgpp pecrgkslts kvpptvqkpt 301 tvnvpttevs ptsqktttkt ttpnaqatrs tpvsrttkhf hettpnkgsg ttsgttrlls 361 ghtcftltgl lgtlvtmgll t

[0200] Gene ID: Y14 Gene symbol: CLDN16 Gene description: claudin 16 Unigene: Hs.251391 Genbank: BC069682 Entrez Gene: 10686 Refseq: NM_006580 Protein sequence (SEQ ID NO: 78): 1 mtsrtpllvt aclyysycns rhlqqgvrks krpvfshcqv petqktdtrh lsgaragvcp 61 chpdgllat mrdllqyiac ffaffsagfl ivatwtdcwm vnaddslevs tkcrglwwec 121 vtnafdgirt cdeydsilae hplklvvtra lmitadilag fgfltlllgl dcvkflpdep 181 yikvricfva gatlliagtp giigsvwyav dvyverstlv lhniflgiqy kfgwscwlgm 241 agslgcflag avltcclylf kdvgpernyp yslrkaysaa gvsmaksysa prtetakmya 301 vdtrv

[0201] Gene ID: Y15 Gene symbol: LAMA3 Gene description: laminin, alpha 3 Unigene: Hs.436367 Genbank: AY327115 Entrez Gene: 3909 Refseq: NM_198129|NM_001127717|NM_000227|NM_001127718 Protein Sequence Isoform 1 (SEQ ID NO: 79): 1 maaaarprgr algpvlpptp llllvlrvlp acgatardpg aaaglslhpt yfnlaeaari 61 watatcgerg pgegrpqpel ycklvggpta pgsghtiqgq fcdycnsedp rkahpvtnai 121 dgserwwqsp plssgtqynr vnltldlgql fhvayilikf ansprpdlwv lersvdfgst 181 yspwqyfahs kvdclkefgr eanmavtrdd dvlcvteysr ivplengevv vslingrpga 241 knftfshtlr eftkatnirl rflrtntllg hliskaqrdp tvtrryyysi kdisiggqcv 301 cnghaevcni nnpeklfrce cqhhtcgetc drcctgynqr rwrpaaweqs heceacnchg 361 hasncyydpd verqqaslnt qgiyagggvc incqhntagv nceqcakgyy rpygvpvdap 421 dgcipcscdp ehadgceqgs grchckpnfh gdncekcaig yynfpfclri pifpvstpss 481 edpvagdikg cdcnlegvlp eicdahgrcl crpgvegprc dtcrsgfysf picqacwcsa 541 lgsyqmpcss vtgqcecrpg vtgqrcdrcl sgaydfphcq gsssacdpag tinsnlgycq 601 cklhvegptc srckllywnl dkenpsgcse ckchkagtvs gtgecrqgdg dchckshvgg 661 dscdtcedgy faleksnyfg cqgcqcdigg alssmcsgps gvcqcrehvv gkvcqrpenn 721 yyfpdlhhmk yeiedgstpn grdlrfgfdp lafpefswrg yaqmtsvqnd vritlnvgks 781 sgslfrvilr yvnpgteavs ghitiypswg aaqskeiifl pskepafvtv pgngfadpfs 841 itpgiwvaci kaegvlldyl vllprdyyea svlqlpvtep cayagppqen cllyqhlpvt 901 rfpctlacea rhflldgepr pvavrqptpa hpvmvdlsgr evelhlrlri pqvghyvvvv 961 eysteaaqlf vvdvnvkssg svlagqvniy scnysvlcrs avidhmsria myelladadi 1021 qlkghmarfl lhqvciipie efsaeyvrpq vhciasygrf vnqsatcvsl ahetpptali 1081 ldvlsgrpfp hlpqqsspsv dvlpgvtlka pqnqvtlrgr vphlgryvfv ihfyqaahpt 1141 fpaqvsvdgg wpragsfhas fcphvlgcrd qviaegqief disepevaat vkvpegkslv 1201 lvrvlvvpae nydyqilhkk smdkslefit ncgknsfyld pqtasrfckn sarslvafyh 1261 kgalpcechp tgatgphcsp eggqcpcqpn vigrqctrca tghygfprck pcscgrrlce 1321 emtgqcrcpp rtvrpqcevc ethsfsfhpm agcegcncsr rgtieaampe cdrdsgqcrc 1381 kpritgrqcd rcasgfyrfp ecvpcncnrd gtepgvcdpg tgaclckenv egtecnvcre 1441 gsfhldpanl kgctscfcfg vnnqchsshk rrtkfvdmlg whletadrvd ipvsfnpgsn 1501 smvadlqelp atihsaswva ptsylgdkvs sygyltyqa ksfglpgdmv llekkpdvql 1561 tgqhmsiiye etntprpdrl hhgrvhvveg nfrhassrap vsreelmtvl srladvriqg 1621 lyftetqrlt lsevgleeas dtgsgriala veicacppay agdscqgcsp gyyrdhkgly 1681 tgrcvpcncn ghsnqcqdgs gicvncqhnt agehcercqe gyygnavhgs cracpcphtn 1741 sfatgcvvng gdvrcsckag ytgtqcerca pgyfgnpqkf ggscqpcscn sngqlgschp 1801 ltgdcinqep kdsspaeecd dcdscvmtll ndlatmgeql rlvksqlqgl sasaglleqm 1861 rhmetqakdl rnqllnyrsa isnhgskieg lereltdlnq efetlqekaq vnsrkaqtln 1921 nnvnratqsa keldvkiknv irnvhillkq isgtdgegnn vpsgdfsrew aeaqrmmrel 1981 rnrnfgkhlr eaeadkresq lllnrirtwq kthqgenngl ansirdslne yeaklsdlra 2041 rlqeaaaaqak qanglnqene ralgaiqrqv keinslqsdf tkylttadss llqtnialql 2101 meksqkeyek laaslnearq elsdkvrels rsagktslve eaekharslq elakqleeik 2161 rnasgdelvr cavdaataye nilnaikaae daanraasas esalqtvike dlprkaktls 2221 snsdkllnea kmtqkklkqe vspalnnlqq tlnivtvqke vidtnlttlr dglhgiqrgd 2281 idamissaks mvrkanditd evldglnpiq tdverikdty grtqnedfkk altdadnsvn 2341 kltnklpdlw rkiesinqql lplgnisdnm drirelikqa rdaaskvavp mrfngksgve 2401 vrlpndledl kgytslslfl qrpnsrengg tenmfvmylg nkdasrdyig mavvdgqltc 2461 vynlgdreae lqvdqiltks etkeavmdrv kfqriyqfar lnytkgatss kpetpgvydm 2521 dgrnsntlln ldpenvvfyv ggyppdfklp srlsfppykg cielddlnen vlslynfkkt 2581 fnlnttevep crrrkeesdk nyfegtgyar vptqphapip tfgqtiqttv drgllffaen 2641 gdrfislnie dgklmvrykl nselpkergv gdainngrdh siqikigklq krmwinvdvq 2701 ntiidgevfd fstyylggip iairerfnis tpafrgcmkn lkktsgvvrl ndtvgvtkkc 2761 sedwklvrsa sfsrggqlsf tdlglpptdh lqasfgfqtf qpsgilldhq twtrnlqvtl 2821 edgyielsts dsgspifksp qtymdgllhy vsvisdnsgl rlliddqllr nskrlkhiss 2881 srqslrlggs nfegcisnvf vqrlslspev ldltsnslkr dvslggcsln kppflmllkg 2941 strfnktktf rinqllqdtp vasprsvkvw qdacsplpkt qanhgalqfg diptshllfk 3001 lpqellkprs qfavdmqtts srglvfhtgt knsfmalyls kgrlvfalgt dgkklriksk 3061 ekcndgkwht vvfghdgekg rlvvdglrar egslpgnsti sirapvylgs ppsgkpkslp 3121 tnsfvgclkn fqldskplyt psssfgvssc lggplekgiy fseegghvvl ahsvllgpef 3181 klvfsirprs ltgilihigs qpgkhlcvyl eagkvtasmd sgaggtstsv tpkqslcdgq 3241 whsvavtikq hilhleldtd ssytagqipf ppastqeplh lggapanltt lripvwksff 3301 gclrnihvnh ipvpvteale vqgpvslngc pdq

[0202] Protein Sequence Isoform 3 (SEQ ID NO: 80): 1 maaaarprgr algpvlpptp llllvlrvlp acgatardpg aaaglslhpt yfnlaeaari 61 watatcgerg pgegrpqpel ycklvggpta pgsghtiqgq fcdycnsedp rkahpvtnai 121 dgserwwqsp plssgtqynr vnltldlgql fhvayilikf ansprpdlwv lersvdfgst 181 yspwqyfahs kvdclkefgr eanmavtrdd dvlcvteysr ivplengevv vslingrpga 241 knftfshtlr eftkatnirl rflrtntllg hliskaqrdp tvtrryyysi kdisiggqcv 301 cnghaevcni nnpeklfrce cqhhtcgetc drcctgynqr rwrpaaweqs heceacnchg 361 hasncyydpd verqqaslnt qgiyagggvc incqhntagv nceqcakgyy rpygvpvdap 421 dgcipcscdp ehadgceqgs grchckpnfh gdncekcaig yynfpfclri pifpvstpss 481 edpvagdikg cdcnlegvlp eicdahgrcl crpgvegprc dtcrsgfysf picqacwcsa 541 lgsyqmpcss vtgqcecrpg vtgqrcdrcl sgaydfphcq gsssacdpag tinsnlgycq 601 cklhvegptc srckllywnl dkenpsgcse ckchkagtvs gtgecrqgdg dchckshvgg 661 dscdtcedgy faleksnyfg cqgcqcdigg alssmcsgps gvcqcrehvv gkvcqrpenn 721 yyfpdlhhmk yeiedgstpn grdlrfgfdp lafpefswrg yaqmtsvqnd vritlnvgks 781 sgslfrvilr yvnpgteavs ghitiypswg aaqskeiifl pskepafvtv pgngfadpfs 841 itpgiwvaci kaegvlldyl vllprdyyea svlqlpvtep cayagppqen cllyqhlpvt 901 rfpctlacea rhflldgepr pvavrqptpa hpvmvdlsgr evelhlrlri pqvghyvvvv 961 eysteaaqlf vvdvnvkssg svlagqvniy scnysvlcrs avidhmsria myelladadi 1021 qlkghmarfl lhqvciipie efsaeyvrpq vhciasygrf vnqsatcvsl ahetpptali 1081 ldvlsgrpfp hlpqqsspsv dvlpgvtlka pqnqvtlrgr vphlgryvfv ihfyqaahpt 1141 fpaqvsvdgg wpragsfhas fcphvlgcrd qviaegqief disepevaat vkvpegkslv 1201 lvrvlvvpae nydyqilhkk smdkslefit ncgknsfyld pqtasrfckn sarslvafyh 1261 kgalpcechp tgatgphcsp eggqcpcqpn vigrqctrca tghygfprck pcscgrrlce 1321 emtgqcrcpp rtvrpqcevc ethsfsfhpm agcegcncsr rgtieaampe cdrdsgqcrc 1381 kpritgrqcd rcasgfyrfp ecvpcncnrd gtepgvcdpg tgaclckenv egtecnvcre 1441 gsfhldpanl kgctscfcfg vnnqchsshk rrtkfvdmlg whletadrvd ipvsfnpgsn 1501 smvadlqelp atihsaswva ptsylgdkvs sygyltyqa ksfglpgdmv llekkpdvql 1561 tgqhmsiiye etntprpdrl hhgrvhvveg nfrhassrap vsreelmtvl srladvriqg 1621 lyftetqrlt lsevgleeas dtgsgriala veicacppay agdscqgcsp gyyrdhkgly 1681 tgrcvpcncn ghsnqcqdgs gicvncqhnt agehcercqe gyygnavhgs cracpcphtn 1741 sfatgcvvng gdvrcsckag ytgtqcerca pgyfgnpqkf ggscqpcscn sngqlgschp 1801 ltgdcinqep kdsspaeecd dcdscvmtll ndlatmgeql rlvksqlqgl sasaglleqm 1861 rhmetqakdl rnqllnyrsa isnhgskieg lereltdlnq efetlqekaq vnsrkaqtln 1921 nnvnratqsa keldvkiknv irnvhmlnri rtwqkthqge nnglansird slneyeakls 1981 dlrarlqeaa aqakqangln qeneralgai qrqvkeinsl qsdftkyltt adssllqtni 2041 alqlmeksqk eyeklaasln earqelsdkv relsrsagkt slveeaekha rslqelakql 2101 eeikrnasgd elvrcavdaa tayenilnai kaaedaanra asasesalqt vikedlprka 2161 ktlssnsdkl lneakmtqkk lkqevspaln nlqqtlnivt vqkevidtnl ttlrdglhgi 2221 qrgdidamis saksmvrkan ditdevldgl npiqtdveri kdtygrtqne dfkkaltdad 2281 nsvnkltnkl pdlwrkiesi nqqllplgni sdnmdrirel iqqardaask vavpmrfngk 2341 sgvevrlpnd ledlkgytsl slflqrpnsr enggtenmfv mylgnkdasr dyigmavvdg 2401 qltcvynlgd reaelqvdqi ltksetkeav mdrvkfqriy qfarlnytkg atsskpetpg 2461 vydmdgrnsn tllnldpenv vfyvggyppd fklpsrlsfp pykgcieldd lnenvlslyn 2521 fkktfnlntt evepcrrrke esdknyfegt gyarvptqph apiptfgqti qttvdrgllf 2581 faengdrfis lniedgklmv ryklnselpk ergvgdainn grdhsiqiki gklqkrmwin 2641 vdvqntiidg evfdfstyyl ggipiairer fnistpafrg cmknlkktsg vvrlndtvgv 2701 tkkcsedwkl vrsasfsrgg qlsftdlglp ptdhlqasfg fqtfqpsgil ldhqtwtrnl 2761 qvtledgyie lstsdsgspi fkspqtymdg llhyvsvisd nsglrllidd qllrnskrlk 2821 hisssrqslr lggsnfegci snvfvqrlsl spevldltsn slkrdvslgg cslnkppflm 2881 llkgstrfnk tktfrinqll qdtpvasprs vkvwqdacsp lpktqanhga lqfgdiptsh 2941 llfklpqell kprsqfavdm qttssrglvf htgtknsfma lylskgrlvf algtdgkklr 3001 ikskekcndg kwhtvvfghd gekgrlvvdg lraregslpg nstisirapv ylgsppsgkp 3061 kslptnsfvg clknfqldsk plytpsssfg vssclggple kgiyfseegg hvvlahsvll 3121 gpefklvfsi rprsltgili higsqpgkhl cvyleagkvt asmdsgaggt stsvtpkqsl 3181 cdgqwhsvav tikqhilhle ldtdssytag qipfppastq eplhlggapa nlttlripvw 3241 ksffgclrni hvnhipvpvt ealevqgpvs lngcpdq

[0203] Protein Sequence Isoform 2 (SEQ ID NO: 81): 1 mppavrrsac smgwlwifga algqclgyss qqqrvpflqp pgqsqlqasy vefrpsqgcs 61 pgyyrdhkgl ytgrcvpcnc nghsnqcqdg sgicvncqhn tagehcercq egyygnavhg 121 scracpcpht nsfatgcvvn ggdvrcscka gytgtqcerc apgyfgnpqk fggscqpcsc 181 nsngqlgsch pltgdcinqe pkdsspaeec ddcdscvmtl lndlatmgeq lrlvksqlqg 241 lsasaglleq mrhmetqakd lrnqllnyrs aisnhgskie glereltdln qefetlqeka 301 qvnsrkaqtl nnnvnratqs akeldvkikn virnvhillk qisgtdgegn nvpsgdfsre 361 waeaqrmmre lrnrnfgkhl reaeadkres qlllnrirtw qkthqgenng lansirdsln 421 eyeaklsdlr arlqeaaaqa kqanglnqen eralgaiqrq vkeinslqsd ftkylttads 481 sllqtnialq lmeksqkeye klaaslnear qelsdkvrel srsagktslv eeaekharsl 541 qelakqleei krnasgdelv rcavdaatay enilnaikaa edaanraasa sesalqtvik 601 edlprkaktl ssnsdkllne akmtqkklkq evspalnnlq qtlnivtvqk evidtnlttl 661 rdglhgiqrg didamissak smvrkandit devldglnpi qtdverikdt ygrtqnedfk 721 kaltdadnsv nkltnklpdl wrkiesinqq llplgnisdn mdrireliqq ardaaskvav 781 pmrfngksgv evrlpndled lkgytslslf lqrpnsreng gtenmfvmyl gnkdasrdyi 841 gmavvdgqlt cvynlgdrea elqvdqiltk setkeavmdr vkfqriyqfa rlnytkgats 901 skpetpgvyd mdgrnsntll nldpenvvfy vggyppdfkl psrlsfppyk gcielddlne 961 nvlslynfkk tfnlntteve pcrrrkeesd knyfegtgya rvptqphapi ptfgqtiqtt 1021 vdrgllffae ngdrfislni edgklmvryk lnselpkerg vgdainngrd hsiqikigkl 1081 qkrmwinvdv qntiidjevf dfstyylggi piairerfni stpafrgcmk nlkktsgvvr 1141 lndtvgvtkk csedwklvrs asfsrggqls ftdlglpptd hlqasfgfqt fqpsgilldh 1201 qtwtrnlqvt ledgyielst sdsgspifks pqtymdgllh yvsvisdnsg lrlliddqll 1261 rnskrlkhis ssrqslrlgg snfegcisnv fvqrlslspe vldltsnslk rdvslggcsl 1321 nkppflmllk gstrfnktkt frinqllqdt pvasprsvkv wqdacsplpk tqanhgalqf 1381 gdiptshllf klpqellkpr sqfavdmqtt ssrglvfhtg tknsfmalyl skgrlvfalg 1441 tdgkklriks kekcndgkwh tvvfghdgek grlvvdglra regslpgnst isirapvylg 1501 sppsgkpksl ptnsfvgclk nfqldskply tpsssfgvss clggplekgi yfseegghvv 1561 lahsvllgpe fklvfsirpr sltgilihig sqpgkhlcvy leagkvtasm dsgaggtsts 1621 vtpkqslcdg qwhsvavtik qhilhleldt dssytagqip fppastqepl hlggapanlt 1681 tlripvwksf fgclrnihvn hipvpvteal evqgpvslng cpdq

[0204] Protein Sequence Isoform 4 (SEQ ID NO: 82): 1 mppavrrsac smgwlwifga algqclgyss qqqrvpflqp pgqsqlqasy vefrpsqgcs 61 pgyyrdhkgl ytgrcvpcnc nghsnqcqdg sgicvncqhn tagehcercq egyygnavhg 121 scracpcpht nsfatgcvvn ggdvrcscka gytgtqcerc apgyfgnpqk fggscqpcsc 181 nsngqlgsch pltgdcinqe pkdsspaeec ddcdscvmtl lndlatmgeq lrlvksqlqg 241 lsasaglleq mrhmetqakd lrnqllnyrs aisnhgskie glereltdln qefetlqeka 301 qvnsrkaqtl nnnvnratqs akeldvkikn virnvhmlnr irtwqkthqg ennglansir 361 dslneyeakl sdlrarlqea aaqakqangl nqeneralga iqrqvkeins lqsdftkylt 421 tadssllqtn ialqlmeksq keyeklaasl nearqelsdk vrelsrsagk tslveeaekh 481 arslqelakq leeikrnasg delvrcavda atayenilna ikaaedaanr aasasesalq 541 tvikedlprk aktlssnsdk llneakmtqk klkqevspal nnlqqtlniv tvqkevidtn 601 lttlrdglhg iqrgdidami ssaksmvrka nditdevldg lnpiqtdver ikdtygrtqn 661 edfkkaltda dnsvnkltnk lpdlwrkies inqqllplgn isdnmdrire liqqardaas 721 kvavpmrfng ksgvevrlpn dledlkgyts lslflqrpns renggtenmf vmylgnkdas 781 rdyigmavvd gqltcvynlg dreaelqvdq iltksetkea vmdrvkfqri yqfarlnytk 841 gatsskpetp gvydmdgrns ntllnldpen vvfyvggypp dfklpsrlsf ppykgcield 901 dlnenvlsly nfkktfnlnt tevepcrrrk eesdknyfeg tgyarvptqp hapiptfgqt 961 iqttvdrgll ffaengdrfi slniedgklm vryklnselp kergvgdain ngrdhsiqik 1021 igklqkrmwi nvdvqntiid gevfdfstyy lggipiaire rfnistpafr gcmknlkkts 1081 gvvrlndtvg vtkkcsedwk lvrsasfsrg gqlsftdlgl pptdhlqasf gfqtfqpsgi 1141 lldhqtwtrn lqvtledgyi elstsdsgsp ifkspqtymd gllhyvsvis dnsglrllid 1201 dqllrnskrl khisssrqsl rlggsnfegc isnvfvqrls lspevldlts nslkrdvslg 1261 gcslnkppfl mllkgstrfn ktktfrinql lqdtpvaspr svkvwqdacs plpktqanhg 1321 alqfgdipts hllfklpqel lkprsqfavd mqttssrglv fhtgtknsfm alylskgrlv 1381 falgtdgkkl rikskekcnd gkwhtvvfgh dgekgrlvvd glraregslp gnstisirap 1441 vylgsppsgk pkslptnsfv gclknfqlds kplytpsssf gvssclggpl ekgiyfseeg 1501 ghvvlahsvl lgpefklvfs irprsltgil ihigsqpgkh lcvyleagkv tasmdsgagg 1561 tstsvtpkqs lcdgqwhsva vtikqhilhl eldtdssyta gqipfppast qeplhlggap 1621 anlttlripv wksffgclrn ihvnhipvpv tealevqgpv slngcpdq

[0205] Gene ID: Y16 Gene symbol: CD40 Gene description: CD40 molecule Unigene: Hs.472860 Genbank: AB209660 Entrez Gene: 958 Refseq: NM_001250|NM_152854 Protein Sequence Isoform 1 (SEQ ID NO: 83): 1 mvrlplqcvl wgclltavhp epptacrekq ylinsqccsl cqpgqklvsd cteftetecl 61 pcgesefldt wnrethchqh kycdpnlglr vqqkgtsetd tictceegwh ctseacescv 121 lhrscspgfg vkqiatgvsd ticepcpvgf fsnvssafek chpwtscetk dlvvqqagtn 181 ktdvvcgpqd rlralvvipi ifgilfaill vlvfikkvak kptnkaphpk qepqeinfpd 241 dlpgsntaap vqetlhgcqp vtqedgkesr isvqerq

[0206] Protein Sequence Isoform 2 (SEQ ID NO: 84): 1 mvrlplqcvl wgclltavhp epptacrekq ylinsqccsl cqpgqklvsd cteftetecl 61 pcgesefldt wnrethchqh kycdpnlglr vqqkgtsetd tictceegwh ctseacescv 121 lhrscspgfg vkqiatgvsd ticepcpvgf fsnvssafek chpwtrspgs aespggdphh 181 lrdpvchplg aglyqkggqe anq

[0207] Gene ID: Y17 Gene symbol: COL17A1 Gene description: Collagen, type XVII, alpha 1 Unigene: Hs.117938 Genbank: AL138761 Entrez Gene: 1308 Refseq: NM_000494 Protein sequence (SEQ ID NO: 85): 1 mdvtkknkrd gtevterivt etvttrltsl ppkggtsngy aktaslgggs rlekqslthg 61 ssgyinstgs trghastssy rrahspastl pnspgstfer kthvtrhaye gsssgnsspe 121 yprkefasss trgrsqtres eirvrlqsas pstrwteldd vkrllkgsrs asvsptrnss 181 ntlpipkkgt vetkivtass qsvsgtydat ildanlpshv wsstlpagss mgtyhnnmtt 241 qsssllntna ysagsvfgvp nnmascsptl hpglstsssv fgmqnnlaps lttlshgttt 301 tstaygvkkn mpqspaavnt gvstsaactt svqsddllhk dckflilekd ntpakkemel 361 limtkdsgkv ftaspasiaa tsfsedtlkk ekqaaynads glkaeangdl ktvstkgktt 421 tadihsygss ggggsggggg vggagggpwg papawcpcgs ccswwkwllg llltwllllg 481 llfglialae evrklkarvd elerirrsil pygdsmdrie kdrlqgmapa agadldkigl 541 hsdsqeelwm fvrkklmmeq engnlrgspg pkgdmgspgp kgdrgfpgtp gipgplghpg 601 pqgpkgqkgs vgdpgmegpm gqrgregpmg prgeagppgs gekgergaag epgphgppgv 661 pgsvgpkgss gspgpqgppg pvglqglrge vglpgvkgdk gpmgppgpkg dqgekgprgl 721 tgepgmrglp gavgepgakg amgpagpdgh qgprgeqglt gmpgirgppg psgdpgkpgl 781 tgpqgpqglp gtpgrpgikg epgapgkivt segssmltvp gppgppgamg ppgppgapgp 841 agpaglpghq evlnlqgppg ppgprgppgp sipgppgprg ppgeglpgpp gppgsflsns 901 etflsgppgp pgppgpkgdq gppgprghqg eqglpgfsts gsssfglnlq gppgppgpqg 961 pkgdkgdpgv pgalgipsgp seggssstmy vsgppgppgp pgppgsisss gqeiqqyise 1021 ymqsdsirsy lsgvqgppgp pgppgpvtti tgetfdysel ashvvsylrt sgygvslfss 1081 sissedilav lqrddvrqyl rqylmgprgp pgppgasgdg sllsldyael ssrilsymss 1141 sgisiglpgp pgppglpgts yeellsllrg sefrgivgpp gppgppgipg nvwssisved 1201 lssylhtagl sfipgppgpp gppgprgppg vsgalatyaa ensdsfrsel isyltspdvr 1261 sfivgppgpp gpqgppgdsr llstdashsr gssssshsss vrrgssysss mstggggags 1321 lgaggafgea agdrgpygtd igpgggygaa aeggmyagng gllgadfagd ldynelavrv 1381 sesmqrqgll qgmaytvqgp pgqpgpqgpp giskvfsays nvtadlmdff qtygaiqgpp 1441 gqkgemgtpg pkgdrgpagp pghpgppgpr ghkgekgdkg dqvyagrrrr rsiavkp

[0208] Gene ID: Y18 Gene symbol: DSC2 Gene description: Desmocollin-2 Unigene: Hs.95612 Genbank: BC063291 Entrez Gene: 1824 Refseq: NM_024422|NM_004949 Protein Sequence Isoform Dsc2a (SEQ ID NO: 86): 1 meaarpsgsw ngalcrllll tlailifasd acknvtlhvp skldaeklvg rvnlkecfta 61 anlihssdpd fqiledgsvy ttntillsse krsftillsn tenqekkkif vflehqtkvl 121 kkrhtkekvl rrakrrwapi pcsmlenslg pfplflqqvq sdtaqnytiy ysirgpgvdq 181 eprnlfyver dtgnlyctrp vdreqyesfe iiafattpdg ytpelplpli ikiedendny 241 pifteetytf tifencrvgt tvgqvcatdk depdtmhtrl kysiigqvpp sptlfsmhpt 301 tgvitttssq ldrelidkyq lkikvqdmdg qyfglqttst ciiniddvnd hlptftrtsy 361 vtsveentvd veilrvtved kdlvntanwr anytilkgne ngnfkivtda ktnegvlcvv 421 kplnyeekqq milqigvvne apfsreaspr samstatvtv nvedqdegpe cnppiqtvrm 481 kenaevgtts ngykaydpet rsssgirykk ltdptgwvti dentgsikvf rsldreaeti 541 kngiynitvl asdqggrtct gtlgiilqdv ndnspfipkk tviickptms saeivavdpd 601 epihgppfdf slesstsevq rmwrlkaind taarlsyqnd ppfgsyvvpi tvrdrlgmss 661 vtsldvtlcd citendcthr vdpriggggv qlgkwailai llgiallfci lftlvcgasg 721 tskqpkvipd dlaqqnlivs nteapgddkv ysangfttqt vgasaqgvcg tvgsgikngg 781 qetiemvkgg hqtsescrga ghhhtldscr gghtevdncr ytysewhsft qprlgekvyl 841 cnqdenhkha qdyvltynye grgsvagsvg ccserqeedg lefldnlepk frtlaeacmk 901 r

[0209] Protein sequence Isoform Dsc2b (SEQ ID NO: 87): 1 meaarpsgsw ngalcrllll tlailifasd acknvtlhvp skldaeklvg rvnlkecfta 61 anlihssdpd fqiledgsvy ttntillsse krsftillsn tenqekkkif vflehqtkvl 121 kkrhtkekvl rracrrwapi pcsmlenslg pfplflqqvq sdtaqnytiy ysirgpgvdq 181 eprnlfiver dtgnlyctrp vdreqyesfe iiafattpdg ytpelplpli ikiedendny 241 pifteetytf tifencrvgt tvgqvcatdk depdtmhtrl kysiigqvpp sptlfsmhpt 301 tgvittcsq ldrelidkyq lkikvqdmdg qyfglqttst ciiniddwnd hlptftrtsy 361 vtsveentvd veilrvtved kdlvntanwr anytilkgne ngnfkivtda ktnegvlcvv 421 kplnyeekqq milqigvne apfsreaspr samstatvtv nvedqdegpe cnppiqtvrm 481 kenaevgtts ngykaydpet rsssgirykk ltdptgwvti dentgsikvf rsldreaeti 541 kngiynitvl asdqggrtct gtlgiilqdv ndnspfipkk tviickptms saeivavdpd 601 epihgppfdf slesstsevq rmwrlkaind taarlsyqnd ppfgsyvvpi tvrdrlgmss 661 vtsldvtlcd citendcthr vdpriggggv qlgquailai llgiallfci lftlvcgasg 721 tskqpkvipd dlaqqnlivs nteapgddkv ysangfttqt vgasaqgvcg tvgsgikngg 781 qetiemvkgg hqtsescrga ghhhtldscr gghtevdncr ytysewhsft qprlgeesir 841 ghtlikn

[0210] Gene ID: Y19 Gene symbol: DSC1 Gene description: Desmocollin-1 Unigene: Hs.567260 Genbank: X72925 Entrez Gene: 1823 Refseq: NM_024421|NM_004948 Protein sequence isoform Dsc1a (SEQ ID NO: 88): 1 malasaapgs ifckqllfsl lvltllcdac qkvylrvpsh lqaetlvgkv nleeclksas 61 lirssdpafr iledgsiytt hdlilsserk sfsiflsdgq rreqqeikvv lsarenkspk 121 krhtkdtalk rskrrwapip aslmenslgp fpqhvqqiqs daaqnytify sisgpgvdke 181 pfnlfyiekd tgdifctrsi drekyeqfal ygyattadgy apeyplplii kieddndnap 241 yfehrvtift vpencrsgts vgkvtatdld epdtlhtrlk ykilqqipdh pkhfsihpdt 301 gvittttpfl drekcdtyql imevrdmggq pfglfntgti tisledendn ppsftetsyv 361 teveenridv eilrmkvqdq dlpntphska vykilqgnen gnfiistdpn tnegvlcvvk 421 plnyevnrqv ilqvgvinea qfskaassqt ptmctttvtv kiidsdegpe chppvkviqs 481 qdgfpagqel lgykaldpei ssgeglryqk lgdednwfei nqhtgdlrtl kvldreskfv 541 knnqynisvv avdavgrsct gtlvvhlddy ndhapqidke vticqnnedf avlkpvdpdg 601 pengppfqff ldnsasknwn ieekdgktai lrqrqnldyn yysvpiqikd rhglvathml 661 tvrvcdcstp secrmkdkst rdvrpnvilg rwailamvlg svlllcilft cfcvtakrtv 721 kkcfpediaq qnlivsnteg pgeevteani rlpmqtsnic dtsmsvgtvg gqgiktqqsf 781 emvkggytld snkggghqtl esvkgvgqgd tgryaytdwq sftqprlgek vylcgqdeeh 841 khcedyvcsy nyegkgslag svgccsdrqe eeglefldhl epkfrtlakt cikk

[0211] Protein sequence isoform Dsc1b (SEQ ID NO: 89): 1 malasaapgs ifckqllfsl lvltllcdac qkvylrvpsh lqaetlvgkv nleeclksas 61 lirssdpafr iledgsiytt hdlilsserk sfsiflsdgq rreqqeikvv lsarenkspk 121 krhtkdtalk rskrrwapip aslmenslgp fpqhvqqiqs daaqnytify sisgpgvdke 181 pfnlfyiekd tgdifctrsi drekyeqfal ygyattadgy apeyplplii kieddndnap 241 yfehrvtift vpencrsgts vgkvtatdld epdtlhtrlk ykilqqipdh pkhfsihpdt 301 gvittttpfl drekcdtyql imevrdmggq pfglfntgti tisledendn ppsftetsyv 361 teveenridv eilrmkvqdq dlpntphska vykilqgnen gnfiistdpn tnegvlcvvk 421 plnyevnrqv ilqvgvinea qfskaassqt ptmctttvtv kiidsdegpe chppvkviqs 481 qdgfpagqel lgykaldpei ssgeglryqk lgdednwfei nqhtgdlrtl kvldreskfv 541 knnqynisvv avdavgrsct gtlvvhlddy ndhapqidke vticqnnedf avlkpvdpdg 601 pengppfqff ldnsasknwn ieekdgktai lrqrqnldyn yysvpiqikd rhglvathml 661 tvrvcdcstp secrmkdkst rdvrpnvilg rwailamvlg svlllcilft cfcvtakrtv 721 kkcfpediaq qnlivsnteg pgeevteani rlpmqtsnic dtsmsvgtvg gqgiktqqsf 781 emvkggytld snkggghqtl esvkgvgqgd tgryaytdwq sftqprlgee sirghtlikn

[0212] Gene ID: Y20 Gene symbol: ITGA6 Gene description: integrin alpha-6 Unigene: Hs.133397 Genbank: X59512 Enter Gene: 3655 Refseq: NM_000210|NM_001079818 protein sequence b 1 maaagqlcll ylsagllsrl gaafnldtre dnvirkygdp gslfgfslam hwqlqpedkr 61 lllvgaprae alplqranrt gglyscdita rgpctriefd ndadptsesk edqwmgvtvq 121 sqgpggkvvt cahryekrqh vntkqesrdi fgrcyvlsqn lrieddmdgg dwsfcdgrlr 181 ghekfgscqq gvaatftkdf hyivfgapgt ynwkgivrve qknntffdmn ifedgpyevg 241 getehdeslv pvpansylgf sldsgkgivs kdeitfvsga pranhsgavv llkrdmksah 301 llpehifdge glassfgydv avvdlnkdgw qdivigapqy fdrdgevgga vyvymnqqgr 361 wnnvkpirln gtkdsmfgia vknigdinqd gypdiavgap yddlgkvfiy hgsangintk 421 ptqvlkgisp yfgysiagnm dldrnsypdv avgslsdsvt ifrsrpvini qktitvtpnr 481 idlrqktacg apsgiclqvk scfeytanpa gynpsisivg tleaekerrk sglssrvqfr 541 nqgsepkytq eltlkrqkqk vcmeetlwlq dnirdklrpi pitasveiqe pssrrrvnsl 601 pevlpilnsd epktahidvh flkegcgddn vcnsnlkley kfctregnqd kfsylpiqkg 661 vpelvlkdqk dialeitvtn spsnprnptk dgddaheakl iatfpdtlty sayrelrafp 721 ekqlscvanq ngsqadcelg npfkrnsnvt fylvlsttev tfdtpdldin lklettsnqd 781 nlapitakak vvielllsvs gvakpsqvyf ggtvvgeqam ksedevgsli eyefrvinlg 841 kpltnlgtat lniqwpkeis ngkwllylvk veskglekvt cepqkeinsl nlteshnsrk 901 kreitekqid dnrkfslfae rkyqtlncsv nvncvnircp lrgldskasl ilrsrlwnst 961 fleeysklny ldilmrafid vtaaaenirl pnagtqvrvt vfpsktvaqy sgvpwwiilv 1021 ailagilmla llvfilwkcg ffkrnkkdhy datyhkaeih aqpsdkerlt sda

[0213] Protein sequence isoform a (SEQ ID NO: 91): 1 maaagqlcll ylsagllsrl gaafnldtre dnvirkygdp gslfgfslam hwqlqpedkr 61 lllvgaprae alplqranrt gglyscdita rgpctriefd ndadptsesk edqwmgvtvq 121 sqgpggkvvt cahryekrqh vntkqesrdi fgrcyvlsqn lrieddmdgg dwsfcdgrlr 181 ghekfgscqq gvaatftkdf hyivfgapgt ynwkgivrve qknntffdmn ifedgpyevg 241 getehdeslv pvpansylgf sldsgkgivs kdeitfvsga pranhsgavv llkrdmksah 301 llpehifdge glassfgydv avvdlnkdgw qdivigapqy fdrdgevgga vyvymnqqgr 361 wnnvkpirln gtkdsmfgia vknigdinqd gypdiavgap yddlgkvfiy hgsangintk 421 ptqvlkgisp yfgysiagnm dldrnsypdv avgslsdsvt ifrsrpvini qktitvtpnr 481 idlrqktacg apsgiclqvk scfeytanpa gynpsisivg tleaekerrk sglssrvqfr 541 nqgsepkytq eltlkrqkqk vcmeetlwlq dnirdklrpi pitasveiqe pssrrrvnsl 601 pevlpilnsd epktahidvh flkegcgddn vcnsnlkley kfctregnqd kfsylpiqkg 661 vpelvlkdqk dialeitvtn spsnprnptk dgddaheakl iatfpdtlty sayrelrafp 721 ekqlscvanq ngsqadcelg npfkrnsnvt fylvlsttev tfdtpdldin lklettsnqd 781 nlapitakak vvielllsvs gvakpsqvyf ggtvvgeqam ksedevgsli eyefrvinlg 841 kpltnlgtat lniqwpkeis ngkwllylvk veskglekvt cepqkeinsl nlteshnsrk 901 kreitekqid dnrkfslfae rkyqtlncsv nvncvnircp lrgldskasl ilrsrlwnst 961 fleeysklny ldilmrafid vtaaaenirl pnagtqvrvt vfpsktvaqy sgvpwwiilv 1021 ailagilmla llvfilwkcg ffkrsrydds vpryhavrir keereikdek yidnlekkqw 1081 itkwnenesy s

[0214] Gene ID: Y21 Gene symbol: ITGB4 Gene description: integrin beta-4 Unigene: Hs.632226 Genbank: X53587| X51841| X52186 Entrez Gene: 3691 Refseq: NM_000213|NM_001005619|NM_001005731 Protein Sequence Isoform 1 (SEQ ID NO: 92): 1 magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr 61 cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel 121 evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv 181 pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail 241 qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr 301 tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle 361 eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg 421 thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv 481 csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn 541 fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr 601 chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde 661 lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll 721 llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr 781 sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec 841 aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk 901 ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql 961 lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021 ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081 vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141 rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201 aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261 nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321 krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddtg cgwkfepllg 1381 eeldlrrvtw rlppeliprl sassgrssda eaphgppddg gaggkggslp rsatpgppge 1441 hlvngrmdfa fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh 1501 shsttlprdy stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy 1561 sveyqllngg elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities 1621 qvhpqsplcp lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem 1681 aqgggpataf rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg 1741 pfpqlgsrag lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef 1801 vsrtlttsgt lsthmdqqff qt

[0215] Protein Sequence Isoform 2 (SEQ ID NO: 93): 1 magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr 61 cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel 121 evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv 181 pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail 241 qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr 301 tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle 361 eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg 421 thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv 481 csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn 541 fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr 601 chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde 661 lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll 721 llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr 781 sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec 841 aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk 901 ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql 961 lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021 ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081 vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141 rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201 aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261 nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321 krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381 fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy 1441 stltsvsshg lppiwehgrs rlplswalgs rsraqmkgfp psrgprdsii lagrpaapsw 1501 gpdsrltagv pdtptrlvfs algptslrvs wqeprcerpl qgysveyqll nggelhrlni 1561 pnpaqtsvvv edllpnhsyv frvraqsqeg wgreregvit iesqvhpqsp lcplpgsaft 1621 lstpsapgpl vftalspdsl qlswerprrp ngdivgylvt cemaqgggpa tafrvdgdsp 1681 esrltvpgls envpykfkvq arttegfgpe regitiesq dggpfpqlgs raglfqhplq 1741 seyssittth tsatepflvd gltlgaqhle aggsltrhvt qefvsrtltt sgtlsthmdq 1801 qffqt

[0216] Protein Sequence Isoform 3 (SEQ ID NO: 94): 1 magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr 61 cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel 121 evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv 181 pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail 241 qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr 301 tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle 361 eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg 421 thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv 481 csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn 541 fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr 601 chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde 661 lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll 721 llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr 781 sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec 841 aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk 901 ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql 961 lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021 ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081 vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141 rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201 aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261 nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321 krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381 fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy 1441 stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy sveyqllngg 1501 elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities qvhpqsplcp 1561 lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem aqgggpataf 1621 rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg pfpqlgsrag 1681 lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef vsrtlttsgt 1741 lsthmdqqff qt

[0217] Gene ID: Y22 Gene symbol: PVRL4 Gene description: Poliovirus receptor-associated protein 4 Unigene: Hs.492490 Genbank: BC010423 Entrez Gene: 81607 Refseq: NM_030916 Protein sequence (SEQ ID NO: 95): 1 mplslgaemw gpeawlllll llasftgrcp ageletsdvv tvvlgqdakl pcfyrgdsge 61 qvgqvawarv dagegaqela llhskyglhv spayegrveq pppprnpldg svllrnavqa 121 degeyecrvs tfpagsfqar lrlrvlvppl pslnpgpale egqgltlaas ctaegspaps 181 vtwdtevkgt tssrsfkhsr saavtsefhl vpsrsmngqp ltcvvshpgl lqdqrithil 241 hvsflaeasv rgledqnlwh igregamlkc lsegqpppsy nwtrldgplp sgvrvdgdtl 301 gfpplttehs giyvchvsne fssrdsqvtv dvldpqedsg kqvdlvsasv vvvgviaall 361 fcllvvvvvl msryhrrkaq qmtqkyeeel tltrensirr lhshhtdprs qpeesvglra 421 eghpdslkdn sscsvmseep egrsystltt vreietqtel lspgsgraee eedqdegikq 481 amnhfvqeng tlrakptgng iyingrghlv

[0218] Gene ID: Y23 Gene symbol: SDC1 Gene description: Syndecan-1 Unigene: Hs.224607 Genbank: BC008765 Entrez Gene: 6382 Refseq: NM_001006946 Protein sequence (SEQ ID NO: 96): 1 mrraalwlwl calalslqpa lpqivatnlp pedqdgsgdd sdnfsgsgag alqditlsqq 61 tpstwkdtql ltaiptspep tgleataast stlpagegpk egeavvlpev epgltareqe 121 atprpretq lptthqastt tattaqepat shphrdmqpg hhetstpagp sqadlhtpht 181 edggpsater aaedgassql paaegsgeqd ftfetsgent avvavepdrr nqspvdqgat 241 gasqglldrk evlggviagg lvglifavcl vgfmlyrmkk kdegsyslee pkqanggayq 301 kptkqeefya

[0219] Gene ID: Z1 Gene symbol: ENPP1 Gene description: Ectonucleotide pyrophosphatase / phosphodiesterase family member 1 Unigene: Hs.527295 Genbank: BC059375 Entrez Gene: 5167 Refseq: NM_006208 Protein sequence (SEQ ID NO: 97): 1 merdgcaggg srggeggrap regpagngrd rgrshaaeap gdpqaaasll apmdvgeepl 61 ekaarartak dpntykvlsl vlsvcvltti lgcifglkps cakevksckg rcfertfgnc 121 rcdaacvelg nccldyqetc iepehiwtcn kfrcgekrlt rslcacsddc kdkgdcciny 181 ssvcqgeksw veepcesine pqcpagfetp ptllfsldgf raeylhtwgg llpvisklkk 241 cgtytknmrp vyptktfpnh ysivtglype shgiidnkmy dpkmnasfsl kskekfnpew 301 ykgepiwvta kyqglksgtf fwpgsdvein gifpdiykmy ngsvpfeeri lavlqwlqlp 361 kderphfytl yleepdssgh sygpvssevi kalqrvdgmv gmlmdglkel nlhrclnlil 421 isdhgmeqgs ckkyiylnky lgdvknikvi ygpaarlrps dvpdkyysfn yegiarnlsc 481 repnqhfkpy lkhflpkrlh faksdriepl tfyldpqwql alnpserkyc gsgfhgsdnv 541 fsnmqalfvg ygpgfkhgie adtfenievy nlmcdllnlt papnngthgs lnhllknpvy 601 tpkhpkevhp lvqcpftrnp rdnlgcscnp silpiedfqt qfnltvaeek iikhetlpyg 661 rprvlqkent icllsqhqfm sgysqdilmp lwtsytvdrn dsfstedfsn clyqdfripl 721 spvhkcsfyk nntkvsygfl sppqlnknss giysealltt nivpmyqsfq viwryfhdtl 781 lrkyaeerng vnvvsgpvfd fdydgrcdsl enlrqkrrvi rnqeilipth ffivltsckd 841 tsqtplhcen ldtlafilph rtdnsescvh gkhdsswvee llmlhrarit dvehitglsf 901 yqqrkepvsd ilklkthlpt fsqed

[0220] Gene ID: Z2 Gene symbol: CD34 Gene description: Hematopoietic progenitor cell antigen CD34 Unigene: Hs.374990 Genbank: M81104 Entrez Gene: 947 Refseq: NM_001773|NM_001025109 Protein sequence isoform b (SEQ ID NO: 98): 1 mlvrrgarag prmprgwtal cllsllpsgf msldnngtat pelptqgtfs nvstnvsyqe 61 tttpstlgst slhpvsqhgn eattnitett vkftstsvit svygntnssv qsqtsvistv 121 fttpanvstp ettlkpslsp gnvsdlstts tslatsptkp ytssspilsd ikaeikcsgi 181 revkltqgic leqnktssca efkkdrgegl arvlcgeeqa dadagaqvcs lllaqsevrp 241 qclllvlanr teissklqlm kkhqsdlkkl gildfteqdv ashqsysqkt lialvtsgal 301 lavlgitgyf lmnrrswspt gerlelep

[0221] Protein sequence isoform a (SEQ ID NO: 99): 1 mlvrrgarag prmprgwtal cllsllpsgf msldnngtat pelptqgtfs nvstnvsyqe 61 tttpstlgst slhpvsqhgn eattnitett vkftstsvit svygntnssv qsqtsvistv 121 fttpanvstp ettlkpslsp gnvsdlstts tslatsptkp ytssspilsd ikaeikcsgi 181 revkltqgic leqnktssca efkkdrgegl arvlcgeeqa dadagaqvcs lllaqsevrp 241 qclllvlanr teissklqlm kkhqsdlkkl gildfteqdv ashqsysqkt lialvtsgal 301 lavlgitgyf lmnrrswspt gerlgedpyy tengggqgys sgpgtspeaq gkasvnrgaq 361 engtgqatsr nghsarqhvv adtel

[0222] Gene ID: Z3 Gene symbol: JAM3 Gene description: Junctional adhesion molecule C Unigene: Hs.150718 Genbank: BC012147 Entrez Gene: 83700 Refseq: NM_032801| NM_001205329 Protein Sequence Isoform 1 (SEQ ID NO: 100): 1 malrrpprlr lcarlpdffl lllfrgclig avnlkssnrt pvvqefesve lsciitdsqt 61 sdpriewkki qdeqttyvff dnkiqgdlag raeilgktsl kiwnvtrrds alyrcevvar 121 ndrkeideiv ieltvqvkpv tpvcrvpkav pvgkmatlhc qeseghprph yswyrndvpl 181 ptdsranprf rnssfhlnse tgtlvftavh kddsgqyyci asndagsarc eeqemevydl 241 niggiiggvl vvlavlalit lgiccayrrg yfinnkqdge syknpgkpdg vnyirtdeeg 301 dfrhkssfvi

[0223] Protein Sequence Isoform 2 (SEQ ID NO: 101): 1 malrrpprlr lcarlpdffl lllfrgclig avnlkssnrt pvvqefesve lsciitdsqt 61 sdpriewkki qdeqttyvff dnkiqvkpvt pvcrvpkavp vgkmatlhcq eseghprphy 121 swyrndvplp tdsranprfr nssfhlnset gtlvftavhk ddsgqyycia sndagsarce 181 eqemevydln iggiiggvlv vlavlalitl giccayrrgy finnkqdges yknpgkpdgv 241 nyirtdeegd frhkssfvi

[0224] Gene ID: Z4 Gene symbol: CD14 Gene description: Monocyte differentiation antigen CD14 Unigene: Hs.163867 Genbank: BC010507 Entrez Gene: 929 Refseq: NM_000591 Protein sequence (SEQ ID NO: 102): 1 merascllll llplvhvsat tpepceldde dfrcvcnfse pqpdwseafq cvsaveveih 61 agglnlepfl krvdadadpr qyadtvkalr vrrltvgaaq vpaqllvgal rvlaysrlke 121 ltledlkitg tmpplpleat glalsslrlr nvswatgrsw laelqqwlkp glkvlsiaqa 181 hspafsceqv rafpaltsld lsdnpglger glmaalcphk fpaiqnlalr ntgmetptgv 241 caalaaagvq phsldlshns lratvnpsap rcmwssalns lnlsfagleq vpkglpaklr 301 vldlscnrln rapqpdelpe vdnltldgnp flvpgtalph egsmnsgvvp acarstlsvg 361 vsgtlvllqg argfa

[0225] Gene ID: Z5 Gene symbol: PLSCR4 Gene description: phospholipid scramblase 4 Unigene: Hs.477869 Genbank: AF199023 Entrez Gene: 57088 Refseq: NM_001128304| NM_001128306| NM_001177304 Protein sequence isoform a (SEQ ID NO: 103): 1 msgvvptape qpagemenqt kppdprpdap peynshflpg ppgtavpppt gypgglpmgy 61 yspqqpstfp lyqpvggihp vryqpgkypm pnqsvpitwm pgptpmancp pgleylvqld 121 nihvlqhfep lemmtcfetn nrydiknnsd qmvyivtedt ddftrnayrt lrpfvlrvtd 181 cmgreimtmq rpfrctcccf ccpsarqele vqcppgvtig fvaehwnlcr avysiqnekk 241 envmrvrgpc stygcgsdsv fevksldgis nigsiirkwn gllsamadad hfdihfpldl 301 dvkmkamifg acflidfmyf ersppqrsr

[0226] Protein sequence isoform b (SEQ ID NO: 104): 1 msgvvptape qpagemenqt kppdprpdap peynshflpg ppgtavpppt gypgglpmgy 61 yspqqpstfp lyqpvggihp vryqpgkypm pnqsvpitwm pgptpmancp pgleylvqle 121 vqcppgvtig fvaehwnlcr avysiqnekk envmrvrgpc stygcgsdsv fevksldgis 181 nigsiirkwn gllsamadad hfdihfpldl dvkmkamifg acflidfmyf ersppqrsr

[0227] Protein sequence isoform c (SEQ ID NO: 105): 1 menqtkppdp rpdappeyns hflpgppgta vppptgypgg lpmgyyspqq pstfplyqpv 61 ggihpvryqp gkypmpnqsv pitwmpgptp mancppgley lvqlevqcpp gvtigfvaeh 121 wnlcravysi qnekkenvmr vrgpcstygc gsdsvfevks ldgisnigsi irkwngllsa 181 madadhfdih fpldldvkmk amifgacfli dfmyferspp qrsr

[0228] Gene ID: Z6 Gene symbol: AMOT Gene description: angiomotin Unigene: Hs.528051 Genbank: AF286598 Entrez Gene: 154796 Refseq: NM_133265|NM_001113490 Protein Sequence Isoform 2 (SEQ ID NO: 106): 1 mpraqpssas yqpvpadpfa ivsraqqmve ilsdenrnlr qelegcyekv arlqkvetei 61 qrvseayenl vkssskreal ekamrnkleg eirrmhdfnr dlrerletan kqlaekeyeg 121 sedtrktisq lfaknkesqr ekekleaela tarstnedqr rhieirdqal snaqakvvkl 181 eeelkkkqvy vdkvekmqqa lvqlqaacek reqlehrlrt rlereleslr iqqrqgncqp 241 tnvseynaaa lmellrekee rilaleadmt kweqkyleen vmrhfaldaa atvaaqrdtt 301 vishspntsy dtaleariqk eeeeilmank rcldmegrik tlhaqiiekd amikvlqqrs 361 rkepskteql scmrpakslm sisnagsgll shsstltgsp imeekrddks wkgslgillg 421 gdyraeyvps tpspvppstp llsahsktgs rdcstqterg tesnktaava pisvpapvaa 481 aataaaitat aatitttmva aapvavaaaa apaaaaapsp ataaataaav spaaagqipa 541 aasvasaaav apsaaaaaav qvapaapapv papalvpvpa paaaqasapa qtqaptsapa 601 vaptpaptpt pavaqaevpa spatgpgphr lsipsltcnp dktdgpvfhs ntlerktpiq 661 ilgqepdaem veyli

[0229] Protein Sequence Isoform 1 (SEQ ID NO: 107): 1 mrnseeqpsg gttvlqrllq eqlrygnpse nrsllaihqq atgngppfps gsgnpgpqsd 61 vlspqdhhqq lvahaarqep qgqeiqsenl imekqlsprm qnneelptye eakvqsqyfr 121 gqqhasvgaa fyvtgvtnqk mrtegrpsvq rlnpgkmhqd eglrdlkqgh vrslserlmq 181 mslatsgvka hppvtsapls ppqpndlykn ptsssefyka qgplpnqhsl kgmehrgppp 241 eypfkgmppq svvckpqepg hfysehrlnq pgrtegqlmr yqhppeygaa rpaqdislpl 301 sarnsqphsp tssltsggsl pllqsppstr lsparhplvp nqgdhsahlp rpqqhflpnq 361 ahqgdhyrls qpglsqqqqq qqqqhhhhhh hqqqqqqqpq qqpgeaysam praqpssasy 421 qpvpadpfai vsraqqmvei lsdenrnlrq elegcyekva rlqkveteiq rvseayenlv 481 kssskreale kamrnklege irrmhdfnrd lrerletank qlaekeyegs edtrktisql 541 601 dkvekmqqal vqlqaacekr eqlehrlrtr lereleslri qqrqgncqpt nvseynaaal 661 mellrekeer ilaleadmtk weqkyleenv mrhfaldaaa tvaaqrdttv ishspntsyd 721 taleariqke eeeilmankr cldmegrikt lhaqiiekda mikvlqqrsr kepskteqls 781 cmrpakslms isnagsglls hsstltgspi meekrddksw kgslgillgg dyraeyvpst 841 pspvppstpl lsahsktgsr dcstqtergt esnktaavap isvpapvaaa ataaaitata 901 atitttmvaa apvavaaaaa paaaaapspa taaataaavs paaagqipaa asvasaaava 961 psaaaaaavq vapaapapvp apalvpvpap aaaqasapaq tqaptsapav aptpaptptp 1021 avaqaevpas patgpgphrl sipsltcnpd ktdgpvfhsn tlerktpiqi lgqepdaemv 1081 eyli

[0230] Gene ID: Z7 Gene symbol: ENPEP Gene description: glutamyl aminopeptidase (aminopeptidase A) Unigene: Hs.435765 Genbank: L12468 Entrez Gene: 2028 Refseq: NM_001977 Protein sequence (SEQ ID NO: 108): 1 mnfaeregsk ryciqtkhva ilcavvvgvg livglavglt rscdssgdgg pgtapapshl 61 psstaspsgp paqdqdicpa sedesgqwkn frlpdfvnpv hydlhvkpll eedtytgtvs 121 isinlsaptr ylwlhlretr itrlpelkrp sgdqvqvrrc feykkqeyvv veaeeeltps 181 sgdglylltm efagwlngsl vgfyrttyte ngqvksivat dheptdarks fpcfdepnkk 241 atytisithp keygalsnmp vakeesvddk wtrttfeksv pmstylvcfa vhqfdsvkri 301 snsgkpltiy vqpeqkhtae yaanitksvf dyfeeyfamn yslpkldkia ipdfgtgame 361 nwglityret nllydpkesa ssnqqrvatv vahelvhqwf gnivtmdwwe dlwlnegfas 421 ffeflgvnha etdwqmrdqm lledvlpvqe ddslmsshpi ivtvttpdei tsvfdgisys 481 kgssilrmle dwikpenfqk gcqmylekyq fknaktsdfw aaleeasrlp vkevmdtwtr 541 qmgypvlnvn gvknitqkrf lldpranpsq ppsdlgytwn ipvkwtedni tssvlfnrse 601 kegitlnssn psgnaflkin pdhigfyrvn yevatwdsia talslnhktf ssadraslid 661 dafalaraql ldykvalnlt kylkreenfl pwqrvisavt yiismfeddk elypmieeyf 721 qgqvkpiads lgwndagdhv tkllrssvlg fackmgdrea lnnasslfeq wlngtvslpv 781 nlrllvyryg mqnsgneisw nytleqyqkt slaqekekll yglasvknvt llsryldllk 841 dtnliktqdv ftviryisyn sygknmawnw iqlnwdylvn rytlnnrnlg rivtiaepfn 901 telqlwqmes ffakypqaga gekpreqvle tvknniewlk qhrntirewf fnllesg

[0231] Gene ID: Z8 Gene symbol: THY1 Gene description: Thy-1 cell surface antigen Unigene: Hs.644697 Genbank: AP003396 Entrez Gene: 7070 Refseq: NM_006288 Protein sequence (SEQ ID NO: 109): 1 mnlaisiall ltvlqvsrgq kvtsltaclv dqslrldcrh entssspiqy efsltretkk 61 hvlfgtvgvp ehtyrsrtnf tskynmkvly lsaftskdeg tytcalhhsg hsppissqnv 121 tvlrdklvkc egisllaqnt swllllllsl sllqatdfms l

Claims

1. A composition enriched in human corneal endothelial cells, said composition comprising human corneal cells expressing at least one marker selected from the group consisting of CD56, CD90, CD166, and coxsackievirus and adenovirus receptor (CAR), and a positive affinity reagent that selectively binds to human corneal endothelial cells compared to cells other than human corneal endothelial cells, said positive affinity reagent being an antibody against a surface protein selected from the group consisting of CD56, CD248, and CAR.

2. The composition of claim 1, wherein the positive affinity reagent is an antibody against CD248 and CAR.

3. 3. The composition of claim 1 or 2, wherein the composition further comprises a second positive affinity reagent different from the positive affinity reagent, the second positive affinity reagent being an antibody against a surface protein selected from the group consisting of CD248 and CAR.

4. The composition of any one of claims 1 to 3, wherein the positive affinity reagent is bound to a label.

5. The composition of claim 4 , wherein the positive affinity reagents are conjugated to different labels.

6. A method for producing a human corneal endothelial cell-enriched composition, comprising: contacting a cell population comprising human corneal cells with a first positive affinity reagent that selectively binds to human corneal endothelial cells compared to human corneal endothelial cells that have undergone fibroblast transformation, and selecting cells to which said first positive affinity reagent binds, wherein said first positive affinity reagent comprises an antibody that binds to the CD56 surface protein; and contacting the cell population comprising human corneal cells with a first negative affinity reagent comprising an antibody that binds to the CD109 surface protein, wherein the first negative affinity reagent selectively binds to human corneal endothelial cells that have undergone fibroblast transformation compared to human corneal endothelial cells; and removing cells to which the first negative affinity reagent is bound; The method comprising:

7. The method of claim 6, wherein the cell population comprising human corneal cells is contacted with the first negative affinity reagent and cells to which the first negative affinity reagent is bound are removed.

8. 8. The method of claim 6 or 7, further comprising contacting a cell population comprising human corneal cells with a second positive affinity reagent that selectively binds to human corneal endothelial cells compared to human corneal endothelial cells that have undergone fibroblast transformation, and selecting cells to which the second positive affinity reagent binds, wherein the second positive affinity reagent comprises an antibody that binds to the CD56 surface protein, an antibody that binds to the CAR surface protein, or an antibody that binds to the CD248 surface protein, and is different from the first positive affinity reagent.

9. A kit comprising an affinity reagent for selecting human corneal endothelial cells, at least one positive affinity reagent consisting of an antibody that binds to the CD56 surface protein, wherein the positive affinity reagent selectively binds to human corneal endothelial cells compared to human corneal endothelial cells that have undergone fibroblast transformation; and A kit comprising a negative affinity reagent that comprises an antibody that binds to the CD109 surface protein and that selectively binds to human corneal endothelial cells that have undergone fibroblast transformation compared to human corneal endothelial cells.

Images