Scan recognition method and scan recognition system based on marshalling in-line grating

Abstract

The present invention discloses a scan recognition method and a scan recognition system based on a marshalling in-line grating, and the scan recognition system includes a moving device, a detection plate, an excitation light source, a marshalling in-line grating, an optical fiber, and a recognition device. A marshalling in-line grating is formed by arranging one end of a plurality of optical fibers in a line at a marshalling interval, or a marshalling in-line grating is formed on an end face of one end of an optical fiber by photolithography or etching. The detection plate is installed on the moving device, and there are detection objects distributed in a matrix on the detection plate. The detection plate is scanned by the relative movement formed by the marshalling in-line grating and the detection plate, and the light emitted or reflected by the detection object is collected by the marshalling in-line grating and transmitted to the recognition device for photoelectric conversion, thereby realizing the scan recognition of the detection plate. This scan recognition method can dynamically and continuously count the detection objects on the detection plate, thereby shortening the recognition time, improving the detection efficiency, and improving the detection accuracy.

Description

[Technical Field]

[0001] The present invention relates to the technical field of detection, realizing digital single molecule scanning, and particularly to a scan recognition method and scan recognition system based on marshalling in-line gratings. [Background technology]

[0002] Single molecule detection (SMD) is an ultrasensitive detection technology that has developed rapidly in recent years. It refers to measuring and analyzing targets at the single-molecule level. It is a new detection method and has opened up a new field of detection. Single molecule fluorescence detection is the most commonly used method for single molecule detection. It tracks changes in the various properties of each fluorescent group in biopolymers to reveal biological information such as interactions between related molecules, enzyme activity, reaction kinetics, molecular conformation, DNA and RNA transcription, and protein folding. Single molecule fluorescence detection has unique applications in chemical analysis, biological analysis, nanomaterial analysis, medical diagnosis, forensic analysis, single-cell analysis, and molecular dynamics mechanism research, and has had a significant impact on the development of many disciplines and fields. There are three types of single molecule fluorescence detection: photon burst detection, single molecule image recording, and single molecule spectral mapping. Photon burst detection is the simplest method and directly measures the number of photons generated in a burst. Single molecule imaging can indicate the position and emission intensity of molecules in an image, and can track and record single molecules in real time. Simoa (Single-molecule Array) technology developed by Quanterix, Inc., USA, is currently the most advanced single molecule fluorescence detection technology, with a sensitivity more than 1000 times that of ELISA technology, and a detection limit reaching fg / mL, realizing effective detection and quantification of single molecules.

[0003] However, conventional Simoa technology requires counting the number of luminescent analytes in the image after imaging, which results in high requirements for imaging equipment and slow detection speed, making it difficult to apply and popularize Simoa technology. Therefore, the present application uses a marshalling in-line grating to dynamically and continuously count the luminescent target molecules, thereby realizing digital single-molecule scanning, thereby improving detection accuracy, shortening recognition time, and improving detection efficiency.

[0004] From the results of the patent search, the patents related to this application are mainly the following:

[0005] 1. A Chinese invention patent with application number "202110018302.1", filing date "2021.01.07", publication number "CN112859256A", publication date "2021.05.28", entitled "Image Recognition-Based Grating Coupler Position Identification and Measurement Method", applicant: Tianjin University. This invention patent discloses an image recognition-based grating coupler position identification and measurement method, specifically including the steps of: (1) identifying the position coordinates of a grating coupler in a chip design; (2) mapping and matching the chip design to the chip dimensions on a measurement platform, thereby obtaining the position coordinates of the grating coupler on the chip on the measurement platform; (3) using a program to control the movement of a displacement table to achieve micron-level identification of the positions of the coupling optical fiber and the grating coupler; and (4) using a spatial scanning method to achieve sub-100-nanometer identification of the positions of the coupling optical fiber and the grating coupler, thereby finding the optimal coupling position and performing chip measurement. However, the patent scans the optical signal of the grating coupler point by point by moving the optical fiber, resulting in low detection efficiency.

[0006] 2. A Chinese invention patent with application number "202111571233.3", filing date "2021.12.21", publication number "CN114240755A", publication date "2022.03.25", entitled "Image super-resolution reconstruction method based on combining optical fiber bundle and micro-scanning technology", applicant: Institute of Optoelectronics Technology, Chinese Academy of Sciences. This invention patent discloses an image super-resolution reconstruction method based on combining optical fiber bundle and micro-scanning technology, and includes a collimating lens, an optical fiber bundle, a displacement driver, a photodetector, a high-voltage amplifier, a displacement control, and a data acquisition device. An object is imaged on its image-side focal plane by the collimating lens and received by the optical fiber bundle. The displacement control device generates a voltage signal, which is amplified by the high-voltage amplifier and then acts on the displacement driver, causing the optical fiber bundle surface array to perform a micro-displacement scan on the image formed by the target object. By precisely adjusting the scan step size of the system, displacement below the diffraction limit of the optical system can be achieved. The present invention utilizes the characteristics of image transmission through the core of the optical fiber bundle and combines it with microscanning technology to overcome the limitation that target detection and recognition is affected by the pixel size of the detector, realizes sub-pixel displacement, compensates for the missing information in the cladding, and uses a photodetector array to quickly capture the optical energy information at the output end of the optical fiber bundle, reduces information loss, and achieves wide-field ultra-high resolution imaging. However, this patent does not detect the optical signal by scanning, but projects the optical signal onto the optical fiber bundle by convex lens imaging, resulting in low detection accuracy.

[0007] 3. A Chinese invention patent bearing application number "200810201668.7", filing date "2008.10.23", publication number "CN101387527B", publication date "2010.12.15", entitled "Optical fiber grating sensing demodulator and its application", applicant: Cao Chungen. This invention patent relates to an optical fiber grating sensing demodulator, which includes a high-power ASE broad-spectrum light source, a micro-opto-mechanical scanning filtering system, an optical coupler, a photodetector, a logarithmic amplifier circuit, a signal collection amplification and analog-to-digital conversion circuit, and a digital signal processing system, wherein the high-power ASE broad-spectrum light source is connected to the micro-opto-mechanical scanning filtering system, the micro-opto-mechanical scanning filtering system is connected to the optical coupler, the optical coupler is connected to the photodetector, and the photodetector is sequentially connected to the logarithmic amplifier circuit, the signal collection amplification and analog-to-digital conversion circuit, and the digital signal processing system. The accuracy of the present invention is greatly improved, and the device is based on micro-optical mechanical scanning filtering technology and DSP signal acquisition and processing technology, which has stable and reliable performance and high measurement accuracy. By remotely reading the reflection wavelength at the center of the optical fiber grating, remote real-time reading of the demodulator can be realized. In the case of a network-type sensing system, the engineering realization is simple and easy to implement. However, the detection in this patent is performed by a fiber Bragg grating sensor, which is different from the scan recognition method using a marshalling in-line grating in the present application. Summary of the Invention [Problem to be solved by the invention]

[0008] SUMMARY OF THE INVENTION The technical problem to be solved by the present invention is to provide a scan recognition method and a scan recognition system based on marshalling in-line grating in order to overcome the deficiencies of the prior art. [Means for solving the problem]

[0009] To solve the above technical problems, the present invention provides the following technical solution: a scan recognition method based on a marshalling in-line grating, in which the marshalling in-line grating comprises a plurality of light collection points arranged linearly at marshalling intervals, the light collection points including light transmission points or reflection points; Detection targets are arranged in a tiled pattern on a detection plate, and the detection plate is scanned by the relative movement formed between the marshalling in-line grating and the detection plate; light emitted or reflected by the detection targets is collected by the light collection points and transmitted to a recognition device for photoelectric conversion, thereby realizing scan recognition of the detection plate; The marshalling in-line grating is used to dynamically and continuously count emitting detection targets, thereby shortening recognition time, improving detection efficiency, and improving detection accuracy; the light collection points are square, circular, or elliptical.

[0010] Furthermore, the detection objects are arranged in a tiled pattern on the detection plate according to a matrix distribution, and the spacing between adjacent light collection points on the marshalling in-line grating is the same as the matrix spacing of the detection objects, so that the light collection points can be aligned with the detection objects, reducing interference caused by misalignment between the light collection points and the detection objects and significantly improving the accuracy of scan recognition.

[0011] Furthermore, the plurality of light collecting points are arranged at intervals along the grating direction X to form a marshalling in-line grating. The light collecting points in the marshalling in-line grating correspond to the detection targets in one row in the matrix on the detection plate. During the relative movement between the marshalling in-line grating and the detection plate, the marshalling in-line grating sequentially scans the detection targets in each row in the matrix on the detection plate.

[0012] Furthermore, adjacent light collecting points are arranged in a staggered pattern perpendicular to the grating direction. The light collecting points in the marshalling in-line grating correspond to detection targets in two adjacent rows in the matrix on the detection plate, thereby increasing the distance between adjacent light collecting points and reducing or avoiding optical interference between detection targets adjacent to the detection targets aligned with the light collecting points, thereby improving detection accuracy.

[0013] Furthermore, the offset distance between adjacent light collection points is equal to the spacing between adjacent light collection points, which is the same as the row and column spacing of the detection target, so that the staggered light collection points in the marshalling in-line grating correspond to the positions of the detection target.

[0014] Furthermore, the direction of relative movement between the marshalling in-line grating and the detection plate is perpendicular to the grating direction.

[0015] Furthermore, the light emitted or reflected by the object to be detected is collected by the marshalling in-line grating and then transmitted to the recognition device via optical fiber. The marshalling in-line grating is installed at one end of the optical fiber, and the recognition device is installed at the other end of the optical fiber. Using optical fiber to transmit the optical signal allows for flexible configuration of the recognition device, making the structure of the scanning recognition system more compact.

[0016] Furthermore, one ends of multiple optical fibers are arranged at intervals along the grating direction X to form light collecting points linearly arranged at marshalling intervals, thereby forming a multi-optical fiber in-line grating or a multi-optical fiber staggered grating. Using one ends of multiple optical fibers as light collecting points not only simplifies the structure of the scan recognition system but also reduces costs.

[0017] Furthermore, a single optical fiber in-line grating or a single optical fiber staggered grating can be formed by forming linearly arranged light collecting points at marshalling intervals on the end face of one end of a single optical fiber by photolithography, etching, or coating. By manufacturing a marshalling in-line grating using one end of a single optical fiber, the number of optical fibers can be reduced, further reducing costs.

[0018] The present invention also relates to a scan recognition system for implementing the scan recognition method, which includes a detection plate and an excitation light source, the excitation light source irradiating the detection plate. The scan recognition system further includes a marshalling in-line grating, an optical fiber, a moving device, and a recognition device, the marshalling in-line grating being installed at one end of the optical fiber, and the recognition device being installed at the other end of the optical fiber, the moving device driving the detection plate or the marshalling in-line grating to achieve relative movement between the marshalling in-line grating and the detection plate, the marshalling in-line grating scanning the detection plate, and the optical signal acquired by the scanning is transmitted to the recognition device via the optical fiber, which converts the optical signal into an electrical signal, thereby realizing scan recognition of the detection plate. The marshalling in-line grating is used to dynamically and continuously count luminescent detection targets, thereby shortening the recognition time, improving detection efficiency, and improving detection accuracy.

[0019] Benefits of the present invention: By using the marshalling in-line grating to dynamically and continuously count the emitting target object, the recognition time can be shortened, the detection efficiency can be improved, and the detection accuracy can be improved. In addition, by manufacturing the marshalling in-line grating with optical fiber, the structure of the scanning recognition system can be simplified and the cost can be reduced. [Brief explanation of the drawings]

[0020] [Figure 1]1 is a perspective structural schematic diagram of a scan recognition system according to a first embodiment; [Figure 2] FIG. 1 is a front view of a scan recognition system according to a first embodiment of the present invention. [Figure 3] FIG. 10 is a perspective structural schematic diagram of a scan recognition system according to a second embodiment. [Figure 4] FIG. 10 is a front view of a scan recognition system according to a second embodiment of the present invention. [Figure 5] FIG. 2 is a schematic diagram of a detection plate. [Figure 6] 1 is a perspective view of a single optical fiber in-line grating according to a first embodiment of the marshalling in-line grating; FIG. [Figure 7] FIG. 10 is a schematic perspective view of the structure of a single optical fiber staggered grating according to a second embodiment of the marshalling in-line grating. [Figure 8] FIG. 10 is a schematic perspective view of the structure of a multi-optical fiber in-line grating according to a third embodiment of the marshalling in-line grating. [Figure 9] FIG. 10 is a front view of a multi-optical fiber in-line grating according to a third embodiment of the marshalling in-line grating. [Figure 10] FIG. 10 is a schematic perspective view of the structure of a multi-optical fiber staggered grating according to Example 4 of the marshalling in-line grating. [Figure 11] FIG. 10 is a front view of a multi-optical fiber staggered grating according to a fourth embodiment of the marshalling in-line grating. [Figure 12] FIG. 1 is a schematic diagram of the scan recognition process 1. [Figure 13] FIG. 1 is a schematic diagram of scan recognition process 2. [Figure 14] Schematic diagram of scan recognition process 3. [Figure 15] FIG. 4 is a schematic diagram of the scan recognition process 4. DETAILED DESCRIPTION OF THE INVENTION

[0021] The present invention will now be further described with reference to specific examples and drawings.

[0022] As shown in FIGS. 1 and 2, a first embodiment of the scan recognition system of the present application includes a moving device 1, a detection plate 2, an excitation light source 3, a marshalling in-line grating 4, an optical fiber 5, and a recognition device 6.

[0023] The target substance 201 to be detected is diluted in a solution and then dispersed and arranged in a tile pattern on the detection plate 2 or test band. The marshalling in-line grating 4 comprises a plurality of light collecting points 401 arranged at intervals along the grating direction X. The light collecting points 401 are square, circular or elliptical.

[0024] The excitation light source 3 and the marshalling in-line grating 4 are installed on the same side of the detection plate 2. The detection plate 2 is installed on a moving device 1, and the marshalling in-line grating 4 may be installed on the moving device 1 so as to form a relative movement between the detection plate 2 or the test band and the marshalling in-line grating 4. Light emitted by the excitation light source 3 irradiates the detection plate 2, exciting a portion of the detection target 201 to which a fluorescent marker is bound, causing it to emit light. When the light collection point 401 is positioned close to the detection target 201, it collects the optical signal emitted by the detection target 201 and transmits it via the optical fiber 5 to the recognition device 6, where the photosensitive tube of the recognition device 6 converts the optical signal collected by the marshalling in-line grating 4 into an electrical signal for calculation or remote display.

[0025] To simplify the structure of the detection element, a marshalling in-line grating 4 can be coupled to one end of an optical fiber 5. The ends of multiple optical fibers 5 can be linearly arranged at marshalling intervals to form light collection points 401 linearly arranged at marshalling intervals, forming a multi-fiber in-line grating 41 or a multi-fiber staggered grating 42. The diameter Q of the optical fiber should be within a range of 0.5 to 1.5 times the maximum dimension d of the object to be detected. The end face of one end of a single optical fiber 5 can be formed with light collection points 401 linearly arranged at marshalling intervals by photolithography, etching, or coating, forming a single-fiber in-line grating 43 or a single-fiber staggered grating 44. The maximum dimension P of the light collection points is within a range of 0.5 to 1.5 times the maximum dimension d of the object to be detected.

[0026] As shown in FIGS. 3 and 4, the second embodiment of the scan recognition system of the present application includes a moving device 1, a detection plate 2, an excitation light source 3, a marshalling in-line grating 4, an optical fiber 5, and a recognition device 6.

[0027] In order to enable alignment of the light collection points 401 with the detection target 201, reduce interference caused by misalignment between the light collection points 401 and the detection target 201, and improve the accuracy of scan recognition, the detection target 201 is arranged in advance on the detection plate 2 or test band according to a matrix arrangement. The number of light collection points 401 on the marshalling in-line grating 4 is equal to or greater than the number of columns in the matrix of the detection target 201 on the detection plate 2, so that each detection target 201 in each row corresponds to one light collection point 401. The maximum dimension of the light collection points 401 is within a range of 0.5 to 1.5 times the maximum dimension d of the detection target, so that the size of the light collection points 401 corresponds to the size of the detection target 201. When the light collecting point 401 is aligned closely to the detection target 201, it ensures that the optical signal collected by the light collecting point 401 is the optical signal emitted by the aligned detection target 201, avoids interference with the optical signals emitted by surrounding detection targets 201, and improves the accuracy of scan recognition and detection. Therefore, the marshalling in-line grating 4 is not elongated, but is square, circular, or elliptical, thereby preventing light emitted by surrounding detection targets 201 aligned with the light collecting point 401 from entering the light collecting point 401 and impairing the detection accuracy.

[0028] As shown in FIG. 5 , the detection plate 2 or test band has the analytes 201 arranged in a matrix on the detection plate 2, with the matrix spacing of the analytes being K. The analytes 201 include molecules of various substances to be detected and analyzed, such as chemical analysis, protein analysis, nucleic acid analysis, cell analysis, exosome analysis, circulating tumor cell analysis, and nanomaterial analysis, and can be applied in the fields of precision medicine, forensic identification, food safety, and environmental protection. Some of the analytes 201 have fluorescent markers bound to them. When the light emitted by the excitation light source 3 is irradiated onto the analytes 201, the fluorescent markers of the analytes 201 are excited and emit fluorescence.

[0029] As shown in FIG. 6, the single optical fiber inline grating of the first embodiment of the marshalling inline grating 4 uses a single optical fiber inline grating 43, and forms light collection points 401 spaced apart and linearly arranged along the grating direction X at one end of a single optical fiber 5 by photolithography, etching, or coating, and the spacing L between adjacent light collection points is the same as the matrix spacing K of the object to be detected or √2 times the matrix spacing K of the object to be detected.

[0030] 7, in the single optical fiber staggered grating of Example 2 of the marshalling in-line grating, the marshalling in-line grating 4 uses a single optical fiber staggered grating 44 to form light collection points 401 spaced apart along the grating direction X by photolithography, etching, or coating at one end of a single optical fiber 5. The spacing L between adjacent light collection points is the same as the row and column spacing K of the detection objects, and the offset distance M between adjacent light collection points is the same as the row and column spacing K of the detection objects or an integer multiple of the row and column spacing K of the detection objects. The light collection points 401 of the single optical fiber staggered grating 44 correspond to two rows of detection objects 201 on the detection plate 2.

[0031] 8 and 9, in the multi-optical fiber in-line grating of Example 3 of the marshalling in-line grating, the marshalling in-line grating 4 uses a multi-optical fiber in-line grating 41, with one end of an optical fiber 5 as a light collection point 401, and one end of a plurality of optical fibers 5 are linearly arranged at intervals L along the grating direction X. The interval L between adjacent light collection points is the same as the matrix interval K of the detection object or is √2 times the matrix interval K of the detection object. Fluorescence from the detection object 201 is collected by one end of the optical fiber 5 and transmitted through the optical fiber 5 to the other end of the optical fiber 5, where it is converted into an electrical signal by a photosensitive tube of the recognition device 6.

[0032] 10 and 11 , in the multi-optical fiber staggered grating of Example 4 of the marshalling in-line grating, the marshalling in-line grating 4 uses a multi-optical fiber staggered grating 42, in which one ends of multiple optical fibers 5 are arranged at intervals along the grating direction X, and one ends of adjacent optical fibers 5 are arranged in a staggered pattern perpendicular to the grating direction X. The interval L between adjacent light-collecting points is the same as the row and column spacing K of the detection objects, and the offset distance M between adjacent light-collecting points is the same as the row and column spacing K of the detection objects, or an integer multiple of the row and column spacing K of the detection objects. The light-collecting points 401 of the multi-optical fiber staggered grating 42, which are one end of the optical fibers 5 of the multi-optical fiber staggered grating 42, correspond to two rows of detection objects 201 on the detection plate 2. This staggered arrangement allows the distance between adjacent light-collecting points 401 to be increased, thereby reducing or avoiding optical interference between adjacent detection objects 201 and those aligned with the light-collecting points 401, thereby improving detection accuracy.

[0033] As shown in FIG. 12, in the scan recognition process 1 of the scan recognition method for a marshalling in-line grating according to the present invention, the target object 201 is diluted in a solution and then dispersed and arranged in a tiled pattern on the detection plate 2. The moving device 1 drives the detection plate 2 to move relative to the marshalling in-line grating 4. At the same time, light emitted from the excitation light source 3 is irradiated onto the detection plate 2 or the test band, exciting and causing a portion of the target object 201 bound to a fluorescent marker to emit light. The light collecting point 401 of the multi-fiber in-line grating 41 or single-fiber in-line grating 43 scans the target object 201 arranged in a tiled pattern on the detection plate 2. The light collecting point 401 collects the optical signals emitted by the target object 201 and transmits them via the optical fiber 5 to the recognition device 6. The photosensitive tube of the recognition device 6 converts the optical signals collected by the marshalling in-line grating 4 into electrical signals for counting.

[0034] As shown in FIG. 13 , in the scan recognition process 2 of the marshalling in-line grating scan recognition method of the present application, the moving device 1 drives the detection plate 2 to move relative to the marshalling in-line grating 4. The light collection points 401 of the multi-fiber in-line grating 41 or single-fiber in-line grating 43 correspond to the positions of the target objects 201 distributed in a matrix on the detection plate 2. At the same time, light emitted by the excitation light source 3 irradiates the detection plate 2, exciting some of the target objects 201 bound with fluorescent markers to emit light. When the light collection point 401 is aligned with the target objects 201 in the first row, it collects the light signals emitted by the emitting target objects 201 in the first row and transmits them to the recognition device 6 via the optical fiber 5. The photosensitive tube of the recognition device 6 converts the light signals collected by the marshalling in-line grating 4 into electrical signals for counting. During the relative movement process between the detection plate 2 and the marshalling in-line grating 4, the light collection points 401 of the multi-fiber in-line grating 41 or single-fiber in-line grating 43 sequentially collect the light signals emitted by the detection objects 201 in each row on the detection plate 2, and calculate the number of emitting detection objects 201 on the detection plate 2, thereby realizing dynamic and continuous counting of the detection objects 201, thereby improving the detection accuracy, shortening the recognition time, and improving the detection efficiency.

[0035] 14 , in the scan recognition process 3 of the marshalling in-line grating scan recognition method of the present application, the grating direction X and the detection plate movement direction Y form an oblique angle of 45°. At this time, the distance L between adjacent light collection points is equal to √2 × the matrix spacing K of the detection objects. Therefore, each light collection point 401 of the multi-fiber in-line grating 41 or single-fiber in-line grating 43 corresponds to each target object 201 at a 45° angle within the matrix of target objects 201 on the detection plate 2. During the relative movement between the detection plate 2 and the marshalling in-line grating 4, the light collection points 401 of the multi-fiber in-line grating 41 or single-fiber in-line grating 43 sequentially collect the optical signals emitted by the target objects 201 in each row on the detection plate 2, and calculate the number of emitting target objects 201 on the detection plate 2, thereby achieving dynamic and continuous counting of the target objects 201. This detection method increases the distance between adjacent light collection points 401, reducing or avoiding optical interference between the detection object 201 adjacent to the detection object 201 aligned with the light collection point 401, thereby improving detection accuracy.

[0036] 15 , in the scan recognition process 4 of the marshalling in-line grating scan recognition method of the present application, the marshalling in-line grating 4 uses a multi-fiber staggered grating 42 or a single-fiber staggered grating 44. The light collection points 401 of the multi-fiber staggered grating 42 or the single-fiber staggered grating 44 correspond to the positions of the two rows of detection targets 201 distributed in a matrix on the detection plate 2. When the front light collection point 401 skips and is aligned with half of the detection targets 201 in the first row, it collects optical signals from half of the detection targets 201 in the first row and transmits them to the recognition device 6 via the optical fiber 5. When the front light collection point 401 is aligned with half of the detection targets 201 in the second row, the rear light collection point 401 is aligned with the remaining half of the detection targets 201 in the first row that are not scanned. During the relative movement between the detection plate 2 and the marshalling in-line grating 4, the light collecting points 401 of the multi-fiber staggered grating 42 or the single-fiber staggered grating 44 sequentially collect the optical signals emitted by the target objects 201 in each row on the detection plate 2, and can count the number of emitting target objects 201 on the detection plate 2, thereby realizing dynamic and continuous counting of the target objects 201. The light collecting points 401 of the multi-fiber staggered grating 42 or the single-fiber staggered grating 44 correspond to the target objects 201 in two adjacent rows in the matrix on the detection plate 2, thereby increasing the distance between adjacent light collecting points 401 and reducing or avoiding optical interference between the target objects 201 adjacent to the target objects 201 aligned with the light collecting points 401, thereby improving detection accuracy.

[0037] As described above, the beneficial effects of the present invention are that the use of marshalling in-line gratings to dynamically and continuously count luminescent detection targets shortens the recognition time, improves detection efficiency, and enhances detection accuracy. Furthermore, the use of marshalling in-line gratings fabricated using optical fiber not only simplifies the structure of the scanning recognition system, but also reduces costs.

[0038] The above examples are only used to illustrate the present invention, and do not limit the present invention. Those skilled in the art can make various modifications and variations without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions should also fall within the protection scope of the present invention, and the protection scope of the present invention should be defined in each claim. [Explanation of symbols]

[0039] 1. Mobile Device 2 Detection plate 3. Excitation light source 4 Marshalling Inline Grating 41 Multi-fiber in-line grating 42 Multi-fiber staggered grating 43 Single Optical Fiber Inline Grating 44 Single Optical Fiber Staggered Grating 401 Light Collection Point 5. Optical Fiber 6 recognition device d Maximum size of the object to be detected K: Row spacing of the detected object L spacing between adjacent light collection points M offset distance between adjacent light collection points P is the maximum dimension of the light collection point Q Fiber diameter X grid direction Y Detection plate movement direction

Claims

1. A linear grating scanning recognition method for single molecule detection, The linear grating (4) comprises a plurality of light collecting points (401) arranged linearly along a linear direction (X), each light collecting point (401) being formed on the end of an optical fiber (5) by photolithography, etching or coating, and the light collecting points (401) include light transmitting points or reflecting points, in a scanning recognition method: The detection object (201) is arranged in a tiled pattern on the detection plate (2), scanning the detection plate (2) by the relative movement formed by the linear grating (4) and the detection plate (2); The light emitted or reflected by the object to be detected (201) is collected by the light collection point (401), transmitted to the recognition device (6) via the optical fiber (5) and photoelectrically converted, thereby realizing scanning recognition of the detection plate (2); The spacing (L) between adjacent light collection points of the linear grating (4) in the linear direction (X) is the same as the row and column spacing (K) of the object to be detected; the plurality of light collection points (401) are arranged at intervals along the linear direction (X) of the linear grating (4); The adjacent light collection points (401) are arranged in a staggered pattern perpendicular to the linear direction (X), and the offset distance (M) is equal to the interval (L) between the adjacent light collection points, thereby increasing the distance between the adjacent light collection points (401), thereby reducing or avoiding optical interference between the detection objects (201) aligned with the light collection points (401), and improving detection accuracy; The maximum dimension P of the light collection point (401) is within a range of 0.5 to 1.5 times the maximum dimension d of the detection object (201). The linear grating (4) is used to dynamically and continuously count the detection object (201) for single molecule detection, thereby avoiding interference with light signals emitted by surrounding detection objects (201) and improving the accuracy of scan recognition and detection. A linear grid scanning recognition method characterized by:

2. The relative movement direction (Y) between the linear grating (4) and the detection plate (2) is perpendicular to the linear direction (X); 2. The linear grid scanning recognition method according to claim 1, wherein:

3. The light emitted or reflected by the object to be detected (201) is collected by the linear grating (4) and then transmitted to the recognition device (6) through the optical fiber (5), the linear grating (4) being installed at one end of the optical fiber (5), and the recognition device (6) being installed at the other end of the optical fiber (5); 3. The linear grid scanning recognition method according to claim 2, wherein:

4. One ends of a plurality of optical fibers (5) are arranged at intervals along a linear direction (X) to form spaced apart linearly arranged light collection points (401), forming a multi-optical fiber linear grating (41); 4. The linear grid scanning recognition method according to claim 3, wherein:

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