Vessel, microfluidic device, and diaphragm pump

Abstract

The present invention provides: a container with which the outflow rate of a fluid can be easily controlled and which can function as part of a diaphragm pump; a microfluidic device; and a diaphragm pump.　The present invention is characterized by comprising: a case body 110 which has a cylindrical part and inside which a fluid is sealed; a thin film 200 which blocks an opening on one end side of the cylindrical part and in which an outflow port for a fluid R is formed upon puncturing thereof; and a diaphragm 300 which blocks an opening on the other end side of the cylindrical part.

Description

[Technical Field]

[0001] The present invention relates to a container, a microfluidic device, and a diaphragm pump. [Background technology]

[0002] Conventionally, there is known a technology relating to a microfluidic device using a microfluidic chip in which minute channels and reaction vessels are provided on a small chip and a reaction process or the like is carried out using a small amount of reagent. This technology enables tests that require expensive reagents to be carried out using a small amount of reagent. There is also known a technology relating to a vessel that seals a fluid such as a reagent to be supplied to the microfluidic chip.

[0003] However, with conventional containers, it is difficult to control the flow rate of fluids such as reagents supplied to microfluidic chips, and conventional containers only function as containers for storing fluids. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 9-175538 [Patent Document 2] Japanese Patent Application Publication No. 2017-121970 Summary of the Invention [Problem to be solved by the invention]

[0005] The present invention provides a container, a microfluidic device, and a diaphragm pump that allow easy control of the amount of fluid discharged and that can function as part of a diaphragm pump configuration. [Means for solving the problem]

[0006] The present invention employs the following means to solve the above problems.

[0007] That is, the container of the present invention is a case body having a cylindrical portion and containing a fluid therein; a thin film that closes an opening on one end side of the cylindrical portion and that forms an outlet for a fluid when broken through; a diaphragm that closes an opening on the other end side of the cylindrical portion; The present invention is characterized by comprising:

[0008] According to the present invention, by pressing the diaphragm with the outlet formed in the thin film, the fluid sealed inside the case body can be discharged. Since the fluid is discharged by pressing the diaphragm, the amount of fluid discharged can be easily controlled. Furthermore, the container can also function as a part of the diaphragm pump.

[0009] A lid portion that isolates the diaphragm from an external space may be provided on the other end of the case body on the opposite side of the thin film with the diaphragm interposed therebetween.

[0010] This prevents the diaphragm from being exposed to the outside when the container is stored or transported.

[0011] The lid portion is integrally formed with the case body, and the boundary between the lid portion and the case body is formed by a thin-walled portion, and the lid portion is configured to be detachable from the case body by tearing the thin-walled portion.

[0012] This makes it possible to supply fluid to a microfluidic chip or the like, or to use the container as a diaphragm pump, by detaching the lid from the case body.

[0013] The lid may be provided with a handle for tearing the thin portion.

[0014] This allows the lid to be easily detached from the case body.

[0015] The case body may be made of a material having gas barrier properties.

[0016] The thin film may be made of a material having gas barrier properties.

[0017] Furthermore, the diaphragm may be made of an elastomer material.

[0018] The container can be attached to a microfluidic chip having a mounting portion to which the case body is attached, a protrusion for piercing the thin film, and a fluid flow path.

[0019] In both cases, the thin film is sandwiched between a first guide member and a second guide member having an insertion hole through which the protrusion is inserted, and the first guide member is provided on the diaphragm side, and the second guide member is provided on the opposite side of the first guide member across the thin film.

[0020] As a result, when the container is attached to the microfluidic chip, the insertion holes prevent the protrusions from being displaced, allowing the protrusions to smoothly break through the thin film.

[0021] Preferably, a fluid is sealed in the space between the diaphragm and the first guide member, and the surface of the first guide member facing the diaphragm is configured as an inclined surface whose diameter decreases toward the insertion hole.

[0022] This makes it possible to prevent the fluid from remaining in the container when the fluid flows out due to the diaphragm being pressed.

[0023] The first guide member is preferably made of a hard material, and the second guide member is preferably made of an elastomeric material.

[0024] By adopting such a configuration, the first guide member can enhance its function as a guide that prevents the protrusion from shifting in position, while the second guide member can enhance its function of preventing fluid from leaking to the outside.

[0025] Further, a guide member having an insertion hole through which the protrusion is inserted is provided between the case body and the thin film, and It is also preferable that a fluid is sealed in the space between the diaphragm and the guide member, and that the surface of the guide member facing the diaphragm is configured as an inclined surface whose diameter decreases toward the insertion hole.

[0026] The microfluidic device of the present invention also includes: a microfluidic chip having a mounting portion to which the case body is mounted, a protrusion portion for breaking through the thin film, and a fluid flow path; the container attached to the microfluidic chip by attaching the case body to the attachment portion; The present invention is characterized by comprising:

[0027] Furthermore, the diaphragm pump of the present invention is The microfluidic device described above; a pressing member that presses the diaphragm; an actuator that reciprocates the pressing member; The present invention is characterized by comprising:

[0028] The above configurations may be combined as much as possible. [Effects of the Invention]

[0029] As described above, the present invention can provide a container, a microfluidic device, and a diaphragm pump that can easily control the amount of fluid that flows out and that can function as part of the configuration of a diaphragm pump. [Brief explanation of the drawings]

[0030] [Figure 1] FIG. 1 is a schematic view of a container according to a first embodiment of the present invention. [Figure 2] FIG. 2 is a schematic cross-sectional view of a container according to Example 1 of the present invention. [Figure 3] FIG. 3 is a schematic view showing a state in which the container according to the first embodiment of the present invention is in use. [Figure 4] FIG. 4 is a schematic diagram of a microfluidic chip to which the container according to the first embodiment of the present invention can be applied. [Figure 5] FIG. 5 is a schematic diagram of a microfluidic device and a diaphragm pump to which the container according to the first embodiment of the present invention can be applied. [Figure 6] FIG. 6 is a schematic diagram of a microfluidic device and a diaphragm pump to which the container according to the first embodiment of the present invention can be applied. [Figure 7] FIG. 7 is a schematic cross-sectional view of a container according to a second embodiment of the present invention. [Figure 8] FIG. 8 is a schematic cross-sectional view of a container according to a third embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0031] The following detailed description of the present invention will be given by way of example with reference to the accompanying drawings, although the dimensions, materials, shapes, relative positions, etc. of the components described in the examples are not intended to limit the scope of the present invention unless otherwise specified.

[0032] Example 1 A container, a microfluidic device, and a diaphragm pump according to a first embodiment of the present invention will be described with reference to FIGS. 1 to 6. FIG. 1 is a schematic diagram of a container according to a first embodiment of the present invention. FIG. 1(a) is a plan view of the container according to this embodiment, and FIG. 1(b) is a back view thereof. FIG. 2 is a schematic cross-sectional view of the container according to the first embodiment of the present invention, taken along the line AA in FIG. 1(a). FIG. 3 is a schematic diagram showing a state in which the container according to the first embodiment of the present invention is in use. FIG. 3(a) is a plan view showing a state in which the lid of the container according to this embodiment has been removed, and FIG. 3(b) is a cross-sectional view along the line BB in FIG. 3(a). FIG. 4 is a schematic diagram of a microfluidic chip to which the container according to the first embodiment of the present invention can be applied. FIG. 4(a) is a plan view of a microfluidic chip to which the container according to this embodiment can be applied, and FIG. 4(b) is a cross-sectional view along the line CC in FIG. 4(a). FIG. 5 is a schematic diagram of a microfluidic device and a diaphragm pump (application example 1) to which the container according to the first embodiment of the present invention can be applied. FIG. 5(a) is a schematic cross-sectional view of a microfluidic device to which the container according to this embodiment can be applied, where the container corresponds to FIG. 2 and the microfluidic chip corresponds to FIG. 4(b). FIG. 5(b) is a schematic view of a pump to which the container according to this embodiment can be applied, where the container corresponds to FIG. 3(b) and the microfluidic chip corresponds to FIG. 4(b). FIG. 6 is a schematic view of a microfluidic device and a diaphragm pump (Application Example 2) to which the container according to Example 1 of the present invention can be applied. FIG. 6(a) is a schematic cross-sectional view of a microfluidic device to which the container according to this embodiment can be applied, where the container corresponds to FIG. 2. FIG. 6(b) is a schematic view of a pump to which the container according to this embodiment can be applied, where the container corresponds to FIG. 3(b).

[0033] <Container> In particular, a container 10 according to this embodiment will be described with reference to FIGS. 1 to 3. The container 10 includes a case 100. The material of the case 100 may be selected depending on the fluid R to be sealed in the case 100. For example, if the fluid R is volatile, it is preferable to use a resin material with excellent gas barrier properties as the material for the case 100. Specifically, polyvinylidene chloride, ethylene-vinyl alcohol copolymer resin, etc. can be used as appropriate. Alternatively, the case 100 may have a multilayer structure with a layer of these resin materials with excellent gas barrier properties. The case 100 integrally includes a case body 110 having a cylindrical portion, a lid portion 120, and a handle portion 121. In this embodiment, the cylindrical portion of the case body 110 is formed of a cylindrical portion.

[0034] The container 10 also includes a thin film 200 that closes an opening at one end of the cylindrical portion of the case body 110. The material of this thin film 200 may also be selected depending on the fluid R to be sealed in the case 100. For example, if the fluid R is volatile, it is preferable to use a material with excellent gas barrier properties for the thin film 200. For example, the thin film 200 may be a single-layer film made of aluminum film, plastic film, or the like, or a multi-layer film made of these materials.

[0035] Furthermore, the container 10 is provided with a diaphragm 300 that closes the opening at the other end of the cylindrical portion of the case body 110. This diaphragm 300 is preferably made of an elastomer material, and in particular, silicone rubber, which has excellent chemical stability and biocompatibility. Using silicone rubber can protect the target cells.

[0036] In the container 10 configured as described above, a sealed space is formed by the cylindrical portion of the case body 110, the thin film 200, and the diaphragm 300. A fluid R such as a sample or a reagent is sealed inside this sealed space. Note that the fluid R can be sealed inside the sealed space by pouring the fluid R into the case body 110 with the diaphragm 300 provided, and then attaching the thin film 200.

[0037] The lid 120 is provided on the other end of the case body 110, on the opposite side of the thin film 200 via the diaphragm 300, so as to isolate the diaphragm 300 from the external space. This makes it possible to suppress the evaporation of the fluid R within the sealed space, even if the diaphragm 300 is gas permeable.

[0038] As described above, the lid 120 is integrally formed with the case body 110. The boundary between the lid 120 and the case body 110 is formed by a thin-walled portion. Specifically, the thin-walled portion is formed by providing grooves 131 and 132, each having a circular planar shape, on the front and back surfaces. As a result, when the thin-walled portion is torn, the lid 120 is detached from the case body 110. In this manner, the lid 120 is configured to be detachable from the case body 110. In this embodiment, the lid 120 is provided with a handle 121 for tearing the thin-walled portion. When the user pulls the handle 121, the thin-walled portion is torn and the lid 120 can be removed from the case body 110. This allows the diaphragm 300 to be exposed during use. Note that FIG. 3 shows the lid 120 detached from the case body 110.

[0039] <Microfluidic chip (Application example 1)> In particular, a microfluidic chip 400 to which the container 10 according to this embodiment can be applied will be described with reference to Fig. 4. In Fig. 4(a), see-through portions are indicated by dotted lines.

[0040] The microfluidic chip 400 is a thin plate-like member made of acrylic, glass, resin, etc. The microfluidic chip 400 is provided with a recess 410 as a mounting portion to which the case body 110 of the container 10 is attached. The inner wall surface of the recess 410 is formed of a cylindrical surface, and is configured to fit with the outer peripheral surface of the cylindrical portion of the case body 110.

[0041] The microfluidic chip 400 is provided with a protrusion 420 at the center of the bottom surface of the recess 410 to break through the thin film 200 of the container 10. The microfluidic chip 400 is also provided with a flow path 430 for the fluid R that is connected to the recess 410. The microfluidic chip 400 is further provided with a reservoir 440 that is connected to the flow path 430, and an outlet 450 for the fluid R.

[0042] <Microfluidic Device and Diaphragm Pump (Application Example 1)> With reference to FIG. 5, an example (application example 1) of a microfluidic device and a diaphragm pump to which the container 10 according to this embodiment can be applied will be described.

[0043] FIG. 5(a) shows a microfluidic device 10S according to Application Example 1. The microfluidic device 10S is composed of a microfluidic chip 400 and a container 10. In this application example, the microfluidic chip 400 shown in FIG. 4 is used. The microfluidic device 10S can be obtained by fitting the case body 110 into the recess 410 of the microfluidic chip 400 and attaching the container 10 to the microfluidic chip 400. When the container 10 is attached to the microfluidic chip 400, the thin film 200 of the container 10 is broken by the protrusion 420, forming an outlet for the fluid R.

[0044] FIG. 5(b) shows a diaphragm pump 10T according to Application Example 1. The diaphragm pump 10T includes a microfluidic device 10S and a pressing mechanism 500. When using the microfluidic device 10S as the diaphragm pump 10T, the lid 120 is removed from the case body 110. The pressing mechanism 500 includes a pressing member 510 that presses the diaphragm 300 of the container 10 and an actuator 520 that reciprocates the pressing member 510. Note that the actuator 520 may be implemented using various known technologies, such as a ball screw mechanism, a rack-and-pinion mechanism, a hydraulic mechanism, or a pneumatic mechanism. In the figure, the pressing member 510 indicated by a solid line is separated from the diaphragm 300, and the pressing member 510 indicated by a dotted line is pressed against the diaphragm 300. When the diaphragm 300 is pressed by the pressing member 510, the fluid R sealed in the container 10 is supplied to the flow path 430 of the microfluidic chip 400 from the outlet formed in the thin film 200 (see the dotted arrow). Note that when the pressing member 510 is released from the diaphragm 300, the diaphragm 300 returns to its original state. This also makes it possible to operate the fluid R to flow backward.

[0045] <Microfluidic Devices and Diaphragm Pumps (Application Example 2)> With reference to FIG. 6, an example (application example 2) of a microfluidic device and a diaphragm pump to which the container 10 according to this embodiment can be applied will be described.

[0046] FIG. 6(a) shows a microfluidic device 10SA according to Application Example 2. The microfluidic device 10SA includes a microfluidic chip 400A and a container 10. The microfluidic chip 400A according to this application example has a different configuration from the microfluidic chip 400 shown in FIG. 4 described above. Like the microfluidic chip 400 described above, the microfluidic chip 400A also includes a recess 410 as an attachment portion, a protrusion 420, and a flow channel 430. Unlike the microfluidic chip 400 described above, the microfluidic chip 400A according to Application Example 2 has the recess 410 and the protrusion 420 on both sides of the flow channel 430, and does not include a reservoir 440 or an outlet 450. However, although not shown, the microfluidic chip 400A is provided with a vent hole used to release air from the flow channel 430.

[0047] In this application example, the microfluidic device 10SA can be obtained by fitting the case body 110 into each of the two recesses 410 of the microfluidic chip 400A and attaching two containers 10. When the two containers 10 are attached to the microfluidic chip 400A, the thin film 200 of each container 10 is broken by the protrusion 420, and outlets for the fluids R1 and R2 are formed, respectively.

[0048] FIG. 6(b) shows a diaphragm pump 10TA according to Application Example 2. The diaphragm pump 10TA includes a microfluidic device 10SA and a pair of pressing mechanisms 500. When using the microfluidic device 10SA as a diaphragm pump 10TA, the lid 120 is removed from the case body 110. The configuration of the pressing mechanism 500 is as described in Application Example 1. In this application example, for example, the diaphragms 300 of the two containers 10 are alternately pressed using the two pressing mechanisms 500, thereby causing the fluids R1 and R2 to move back and forth within the flow path 430. When different samples or reagents are used as the fluids R1 and R2, these can be mixed.

[0049] <Advantages of the container, microfluidic device, and diaphragm pump according to this embodiment> According to the container 10 of this embodiment, the fluid sealed inside the case body 110 can be discharged by pressing the diaphragm 300 with the outlet formed in the thin film 200. Since the fluid is discharged by pressing the diaphragm 300, it is easier to control the amount of fluid that flows out compared to when a container that undergoes plastic deformation is used. In addition, the container 10 can also function as a part of the diaphragm pumps 10T and 10TA.

[0050] Before use, the lid 120 is provided on the case 100 of the container 10, so the diaphragm 300 is not exposed to the outside during storage or transportation of the container 10. This prevents the diaphragm 300 from being damaged or being pressed, which could result in fluid leakage. The lid 120 is configured to be detachable from the case body 110, and when the container 10 is to be used as a diaphragm pump 10T, 10TA, it can be simply removed from the case body 110. In this embodiment, the lid 120 can be easily detached from the case body 110 by pulling the handle 121. Furthermore, even if the diaphragm 300 is gas permeable, volatilization of the fluid R within the sealed space before use can be suppressed by forming the case 100 and the thin film 200 from a material with gas barrier properties.

[0051] Then, simply by attaching the container 10 to the microfluidic chip 400, 400A, the fluid sealed in the container 10 can be supplied to the flow channel 430 of the microfluidic chip 400, 400A. Therefore, space can be saved and the number of parts can be reduced.

[0052] The microfluidic devices 10S and 10SA according to the present embodiment can be obtained by attaching the container 10 to the microfluidic chips 400 and 400A. Immediately after the container 10 is attached, the fluid R sealed in the container 10 is ready to be supplied to the flow path 430 provided in the microfluidic chips 400 and 400A, so that it is possible to prevent foreign matter from being mixed into the fluid R.

[0053] Example 2 A container according to Example 2 of the present invention will be described with reference to Fig. 7. In this example, a configuration in which a portion of the container configuration is different from that of Example 1 is shown. Since the basic configuration and operation are the same as those of Example 1, the same components are given the same reference numerals and their description will be omitted as appropriate. Fig. 7 is a schematic cross-sectional view of a container according to Example 2 of the present invention, and corresponds to a cross-sectional view of the container cut at the same location as the cross-sectional view shown in Fig. 2 of Example 1.

[0054] The container 10A according to this embodiment also includes a case 100, a thin film 200A, and a diaphragm 300. The case 100 and the diaphragm 300 are the same as those described in the first embodiment.

[0055] In this embodiment, the configuration of the thin film 200A itself is the same as that described in the first embodiment, but the attachment structure of the thin film 200A to the case body 110 is different from that in the first embodiment. In this embodiment, the thin film 200A is configured to be sandwiched between a first guide member 600 and a second guide member 700, both of which have insertion holes 610, 710 through which the protrusions 420 of the microfluidic chips 400, 400A are inserted. The first guide member 600 is provided on the diaphragm 300 side, and the second guide member 700 is provided on the opposite side of the first guide member 600 across the thin film 200A. The first guide member 600 is fixed to the case body 110. The first guide member 600 is made of a hard material (such as a hard resin material), and the second guide member 700 is made of an elastomer material such as rubber. The container 10A according to this embodiment can be used in place of the container 10 in the microfluidic device and diaphragm pump shown in the first embodiment.

[0056] The container 10A configured as described above can also achieve the same effects as those of Example 1. According to the container 10A of this example, the insertion holes 610 and 710 prevent the protrusions 420 from shifting position when the container 10A is attached to the microfluidic chip, allowing the protrusions 420 to smoothly break through the thin film 200A. Furthermore, since the first guide member 600 is made of a hard material, sufficient force can be applied when attaching the container 10A to the microfluidic chip. This prevents the container 10A from being insufficiently attached. Furthermore, since the second guide member 700 is made of an elastomer material such as rubber, it can be tightly attached to the bottom surface of the recess 410 of the microfluidic chip 400 or 400A. This more reliably prevents fluid from leaking to the outside.

[0057] Example 3 A container according to Example 3 of the present invention will be described with reference to Fig. 8. In this example, a configuration in which the shape of the first guide member is different from that of Example 2 is shown. Since the basic configuration and operation are the same as those of Example 2, the same components are given the same reference numerals and their description will be omitted as appropriate. Fig. 8 is a schematic cross-sectional view of a container according to Example 3 of the present invention, and corresponds to a cross-sectional view of the container cut at the same location as the cross-sectional view shown in Fig. 2 of Example 1.

[0058] The container 10B according to this embodiment also includes a case 100, a thin film 200A, and a diaphragm 300. The case 100 and the diaphragm 300 are the same as those described in the first embodiment.

[0059] In this embodiment, the configuration of the thin film 200A itself is the same as that described in the first embodiment, but the structure for attaching the thin film 200A to the case body 110 is different from that in the first embodiment. In this embodiment, the thin film 200A is configured to be sandwiched between a first guide member 600B and a second guide member 700, both of which have insertion holes 610, 710 through which the protrusions 420 of the microfluidic chips 400, 400A are inserted. The first guide member 600B is provided on the diaphragm 300 side, and the second guide member 700 is provided on the opposite side of the first guide member 600 with the thin film 200A interposed therebetween. The first guide member 600B is fixed to the case body 110. The first guide member 600B is made of a hard material (such as a hard resin material), and the second guide member 700 is made of an elastomer material such as rubber. The container 10B according to this embodiment can be used in place of the container 10 in the microfluidic device and diaphragm pump shown in the first embodiment.

[0060] In the first guide member 600B according to this embodiment, the surface 620 on the diaphragm 300 side is configured as an inclined surface whose diameter decreases toward the insertion hole 610. Note that in this embodiment, the inclined surface is configured as a tapered surface, but the inclined surface may have a configuration other than a tapered surface (for example, an inclined surface that is curved rather than straight in a cross-sectional view).

[0061] The container 10B configured as described above can also achieve the same effects as in Examples 1 and 2. In the present example, since the surface 620 on the diaphragm 300 side is configured as an inclined surface, it is possible to prevent the fluid from remaining in the container when the diaphragm 300 is pressed and the fluid R flows out.

[0062] Furthermore, as a result of the verification, it was found that, depending on the conditions of use, there is no problem in terms of quality even when the container 10B according to this embodiment is configured without the second guide member 700. Therefore, it is also possible to adopt a container that does not include the second guide member 700 in the configuration of the container 10B.

[0063] (others) In each of the above-described embodiments, the case 100 has a configuration including the lid portion 120. However, depending on the type of fluid R to be sealed, the material of the diaphragm 300, and storage and transportation conditions, the lid portion 120 may not be necessary. In this way, depending on various conditions, the lid portion 120 in the case 100 is not essential, and a configuration without the lid portion 120 in the case 100 may be adopted. [Explanation of symbols]

[0064] 10,10A,10B container 10S, 10SA microfluidic device 10T, 10TA diaphragm pump 100 cases 110 Case body 120 Lid 121 Handle 131,132 groove 200,200A thin film 300 diaphragm 400,400A microfluidic chip 410 recess 420 Protrusion 430 Flow path 440 Reservoir 450 outlet 500 Pressing mechanism 510 Pressing member 520 Actuator 610,710 Insertion hole R,R1,R2 Fluid

Claims

1. a case body having a cylindrical portion and containing a fluid therein; a thin film that closes an opening on one end side of the cylindrical portion and that forms an outlet for a fluid when broken through; a diaphragm that closes an opening on the other end side of the cylindrical portion; A container comprising: A container characterized in that a lid portion that isolates the diaphragm from an external space is provided on the other end side of the case body, on the opposite side of the thin film with the diaphragm interposed therebetween.

2. The container according to claim 1, characterized in that the lid portion is integrally formed with the case body, the boundary between the lid portion and the case body is formed by a thin-walled portion, and the lid portion can be detached from the case body by tearing the thin-walled portion.

3. 3. The container according to claim 2, wherein the lid is provided with a handle for tearing the thin portion.

4. 4. The container according to claim 1, wherein the case body is made of a material having gas barrier properties.

5. 4. The container according to claim 1, wherein the thin film is made of a material having gas barrier properties.

6. 4. A container according to claim 1, wherein the diaphragm is made of an elastomer material.

7. 4. The container according to claim 1, which is attached to a microfluidic chip having an attachment portion to which the case body is attached, a protrusion portion for breaking through the thin film, and a fluid flow path.

8. The container according to claim 7, characterized in that the thin film is sandwiched between a first guide member and a second guide member, both of which have insertion holes through which the protrusions are inserted, the first guide member being provided on the diaphragm side, and the second guide member being provided on the opposite side of the first guide member across the thin film.

9. The container according to claim 8, characterized in that a fluid is sealed in the space between the diaphragm and the first guide member, and the surface of the first guide member facing the diaphragm is configured as an inclined surface whose diameter decreases as it approaches the insertion hole.

10. 9. The container of claim 8, wherein the first guide member is made of a hard material and the second guide member is made of an elastomeric material.

11. a guide member having an insertion hole through which the protrusion is inserted is provided between the case body and the thin film; The container according to claim 7, characterized in that a fluid is sealed in the space between the diaphragm and the guide member, and the surface of the guide member facing the diaphragm is configured as an inclined surface whose diameter decreases as it approaches the insertion hole.

12. a microfluidic chip having a mounting portion to which the case body is mounted, a protrusion portion for breaking through the thin film, and a fluid flow path; the container according to any one of claims 1 to 3, which is attached to the microfluidic chip by attaching the case body to the attachment portion; A microfluidic device comprising:

13. The microfluidic device of claim 12; a pressing member that presses the diaphragm; an actuator that reciprocates the pressing member; A diaphragm pump comprising:

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