Centrifugal immunoassay device

Abstract

A centrifugal immunoassay device is provided that can perform both centrifugation and transmission-type detection in a single device by forming a plurality of wells so that both can be performed in a single device. The centrifugal immunoassay device according to the embodiment includes a centrifugal cartridge having a plurality of wells and a sensor connected to the centrifugal cartridge. 、 The device comprises an absorptiometer for measuring the absorbance of the substance to be detected in the detection well, and a syringe for sucking up the substance to be detected and discharging it into the detection well. The absorptiometer includes a light emitting unit that transmits only detection light in a specific wavelength range to the detection target in the detection well. light Shooting The output unit outputs a plurality of detection lights having wavelength bands in different regions. Shooting A plurality of units are installed so as to emit light with a wavelength band of one of the ranges. Examination A light selection section is included that can select and direct the exiting light to the detection target in the detection well.

Description

[Technical Field]

[0001] The present invention provides Centrifugal immunoassay The present invention relates to a device, and more particularly to a centrifugal immunoassay device for multiplexed diagnostics. [Background technology]

[0002] In the prior art, the cartridge has a disk rotation drive structure for automatic measurement. have However, this method relies on disk rotation, so requires a fine flow channel structure, and is troublesome because the upper and lower plates must be joined, making mass production and management difficult. It also requires many parts, which makes the cartridge expensive and makes it difficult to inject the sample. Emits light that Only one diagnosis is possible using an absorptiometer containing a light source that emits a luminescent light.

[0003] Furthermore, in conventional immunoassay devices, the centrifuge and the transmission detector are separate. to and a step of transferring the object to be detected from the centrifuge to the transmission detector is required. For However, there is a problem in that the cost and time required for detection increases.

[0004] therefore The present invention provides an immunoassay device that has a simple structure and can perform centrifugation and transmission type detection in one device using a cartridge with multiple wells, and can perform centrifugation and transmission type detection in one device. Convenience Increased sexuality It is required to In addition, the compact multi-color optical structure allows multiple diagnostics to be performed in one system. required do. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Korean Patent Publication No. 10-2024-0018037 (Published February 13, 2024) Summary of the Invention [Problem to be solved by the invention]

[0006] The present invention has been made to solve the above-mentioned problems. can be The technical problem that the present invention aims to achieve is to provide a centrifugal immunoassay device that can perform both centrifugation and transmission type detection in one device by forming multiple wells so that both can be performed in one cartridge. (Multiple ways) The wavelength of Used measurement By The object is to provide a detection system having an optical structure capable of multiplexed diagnosis.

[0007] The technical problems that the present invention aims to achieve are not limited to the above-mentioned technical problems. figure , not mentioned Technical challenges and Other technical challenges This disclosure It is understood from [Means for solving the problem]

[0008] In order to achieve the above technical objectives, a centrifugal immunoassay device according to one embodiment of the present invention includes a centrifugal cartridge having a plurality of wells in which samples are accommodated, a rotary drive unit on which the centrifugal cartridge is mounted and which rotates, and a rotary drive unit connected to the centrifugal cartridge. Or connect Multiple wells Our An absorptiometer that measures the absorbance of the substance to be detected in the detection well, and a centrifugal cartridge Above to Or facing a centrifugal cartridge and a housing surrounding the centrifugal cartridge, the rotary drive unit, the absorptiometer, and the syringe, and the target substance is extracted from the sample by centrifugation. Separate Centrifugal detection immunoassay Device (Also called a centrifuge type testing device)The absorption spectrophotometer is a specific wavelength region Detected light Only the light is transmitted to the object to be detected in the detection well. Shooting a light detecting unit that measures detection light that has passed through the detection object in the detection well and calculates a detection value, Shooting The output unit outputs a plurality of detection lights having wavelength bands in different regions. Shooting Several units are installed to allow The absorptiometer or the centrifugal immunoassay device is Multiple lights Shooting Debe Multiple detection lights emitted from Inside from Has a wavelength band in any one of the regions Shooting Select Idemitsu Alternatively, a light emitting unit that emits light having a wavelength band in any one of the regions is selected from among a plurality of light emitting units. A light selection unit that can send light to the object to be detected in the detection well moreover It may also contain.

[0009] In an embodiment of the present invention, the sample may be blood, and the substance to be detected may be plasma.

[0010] In an embodiment of the present invention, the detection well is a cuvette capable of transmission detection. of structure have It's okay It may also be a cuvette that allows transmission detection. stomach.

[0011] In an embodiment of the present invention, the centrifugal cartridge may include a sample well in which a sample is contained, a centrifugation well in which the sample is moved from the sample well by rotation and contained therein, and a sample movement passage connecting the sample well and the centrifugation well to form a flow path.

[0012] In an embodiment of the present invention, the syringe has a tip member at its lower end that aspirates the detection target, and the tip member may penetrate the upper surface of the centrifugal cartridge to aspirate the detection target contained in the centrifugation well, and eject the aspirated detection target into the detection well.

[0013] In an embodiment of the present invention, a step is formed at the bottom of the centrifugation well to suck in only the target substance separated from the sample by centrifugation, and the tip member is in the centrifugation well Top of the step Above The object to be detected may be sucked in by inserting the sensor into the sensor.

[0014] In an embodiment of the present invention, a plurality of light Shooting The exit is Yen spaced apart from each other along The light selection unit is arranged in a circle. spaced apart from each other along Multiple lights arranged Shooting A drive motor that rotates the output part and a light Shooting and a position sensor that detects the position of the projection.

[0015] In an embodiment of the present invention, Shooting Debe each is the detection light of a certain wavelength Shooting The color LED that emits light and the color LED that emits light Shooting The emitted detection light is focused Let and a bandpass filter that passes only certain wavelengths. Filter and may include:

[0016] In an embodiment of the present invention, the housing includes a printer, a touch screen, a progress indicator, a power switch, and a door home At least one of them may be provided.

[0017] In an embodiment of the present invention, the touch screen allows the user to perform multiple diagnostics. for A command to operate the centrifugal immunoassay device may be input. [Effects of the Invention]

[0018] The centrifugal immunoassay device according to the embodiment of the present invention includes one centrifugal cartridge. Using By performing centrifugation and transmission detection, the detection process can be completed in a single device. Be One centrifugal immunoassay device Using , one centrifugal cartridge Using Since the detection results can be confirmed after centrifugation and detection, the time and cost required for detection can be effectively reduced.

[0019] Furthermore, the centrifugal immunoassay device according to the embodiment of the present invention includes a plurality of (Multiple ways) wavelength Using the detection light of measurement By The ability to perform multiplexed diagnosis reduces the time and cost required for detection.

[0020] The effects of the present invention are not limited to the above-mentioned effects. Disclosure Includes all effects that can be inferred from [Brief explanation of the drawings]

[0021] [Figure 1] 1 is a perspective view showing a centrifugal immunoassay device according to one embodiment of the present invention, with the housing removed. [Figure 2] 1 is a perspective view showing a centrifugal separation cartridge according to an embodiment of the present invention. [Figure 3] 1 is a cross-sectional view showing a centrifugal separation cartridge according to an embodiment of the present invention. [Figure 4] 1 is a perspective view showing an absorptiometer according to an embodiment of the present invention. [Figure 5] 1 is a conceptual diagram showing an absorptiometer according to one embodiment of the present invention. [Figure 6] 1 is a perspective view showing a centrifugal immunoassay device according to one embodiment of the present invention. [Figure 7] 1 is a perspective view showing a centrifugal immunoassay device according to one embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0022] The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. 、 In order to clearly explain the present invention, unnecessary parts have been omitted and similar parts have been designated by similar reference numerals throughout the specification.

[0023] Throughout the specification, certain parts are said to be "connected" (connected, contacted, or joined) to other parts. In case teeth 、 This includes not only cases where the components are "directly connected" but also cases where the components are "indirectly connected" through the intermediary of other components. be captured Also, when a part is said to "contain" a certain component, case , especially against Meaning of Unless otherwise stated, this does not mean that other components are excluded, but rather that other components may be further included.

[0024] The terms used in this specification are used to describe specific embodiments. can be , which defines the present invention. thing The singular expression includes the plural expression unless the context clearly indicates otherwise. As used herein, the terms "comprise" or "have" and the like mean to indicate the presence of any feature, number, step, operation, component, part, or combination thereof set forth in the specification. intention and one or more other features, numbers, steps, operations, components, parts or combinations thereof. but existence death or can be added That Do not exclude.

[0025] Next, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0026] FIG. 1 shows a centrifugal immunoassay device according to one embodiment of the present invention, with the housing removed. (It can also be called a centrifugal separator type testing device) FIG. 2 is a perspective view showing a centrifugal cartridge according to one embodiment of the present invention. FIG. 3 is a cross-sectional view showing a centrifugal cartridge according to one embodiment of the present invention. FIG. 4 is a perspective view showing an absorptiometer according to one embodiment of the present invention. FIG. 5 is a conceptual diagram showing an absorptiometer according to one embodiment of the present invention. FIGS. 6 and 7 are perspective views showing a centrifugal immunoassay device according to one embodiment of the present invention.

[0027] 1 to 7, a centrifugal immunoassay device 10 according to one embodiment of the present invention is a device for separating a target substance from a sample and detecting the separated target substance. R The centrifugal immunoassay device 10 includes a centrifugal cartridge 100, an absorptiometer 200, a cartridge loading unit 300, a rotational drive unit 400, a horizontal drive unit 500, a syringe 600, a syringe drive unit 700, a housing 800, and a control unit.

[0028] The centrifugal cartridge 100 is an all-in-one cartridge that is mounted on the centrifugal immunoassay device 10, separates the target substance from the sample contained therein through rotation, and detects the separated target substance. R The centrifugal cartridge 100 includes a sample well 110, a centrifugal well 120, a sample transfer path 130, a detection well 140, a cover 150, and a tube cap 160. R .

[0029] The sample well 110 is a space into which a sample is injected by a user before a centrifugation operation is performed, and in which the injected sample is accommodated. The sample well 110 may be a portion of the centrifuge cartridge 100 in which the space is formed. Here, the sample may be whole blood B. As another example, the sample may be a urine sample.

[0030] The centrifugation well 120 moves the sample contained in the sample well 110 by rotating it. death More specifically, the centrifugation well 120 is disposed at a distance from the sample well 110, and the centrifugation well 120 is generated through a rotational motion. death By centrifugal force 、 The centrifugation well 120 may be a space into which the sample accommodated in the sample well 110 moves and is accommodated. The centrifugation well 120 may also be a space into which the sample is accommodated after being centrifuged according to the specific gravity of the substances constituting the sample. The centrifuge well 120 may be the portion of the centrifuge cartridge 100 in which the space is formed.

[0031] this case The sample contained in the sample well 110 can be moved to the centrifugation well 120 via the sample movement path 130. The sample movement path 130 is a flow path formed by connecting the sample well 110 and the centrifugation well 120, and is a path through which the sample can move only in the direction from the sample well 110 to the centrifugation well 120. case The sample transfer passage 130 may be provided at a position above a preset height to prevent the centrifuged sample from transferring back to the sample well 110. Here, the preset height may be the height of the sample contained in the sample well 110 before the centrifugation operation.

[0032] The substances with a high specific gravity in the centrifuged sample can settle at the bottom of the centrifugation well 120, and the substances with a low specific gravity in the centrifuged sample can settle on top of the substances with a high specific gravity. direction to Distribution Placed 、 It can be separated from the precipitated substance. In one embodiment, the substance with a low specific gravity in the centrifuged sample may be the target of detection. Here, the target of detection may be plasma P. In another embodiment, the substance with a high specific gravity in the centrifuged sample may be a sample that is not the target of detection. The sample that is not the target of detection may be red blood cells R.

[0033] The centrifugation well 120 contains only the substance to be detected. but suction And At the bottom , the step shown within the dashed line 121 in FIG. 3 (hereinafter, Step 121 (That is, More specifically, the centrifugation well 120 can form only the target substance to be detected in the centrifuged sample. but suction And A step 121 can be formed at the bottom so that the step 121 is positioned at the bottom.

[0034] The step 121 is a space where the detection target contained in the centrifugal well 120 is sucked by the chip member 610 described later. Part that forms The step 121 is Above It may be a space into which a chip member is inserted and into which a detection target substance is extracted. The top of the step 121 may be a bottom that is shallower than the deepest bottom of the centrifugation well 120 . Step 121 is the upper Above to 、 The predetermined position may be formed so that only the target substance, which is a sample with a low specific gravity among the centrifuged samples, is located. The boundary between the substance with a lower specific gravity The height of the ion beam can be higher than the height of the ion beam.

[0035] The centrifugal separation well 120 has a step 121 formed therein, which allows the tip member 610 to suck in only the substance to be detected, thereby improving reproducibility. of Secure death ,detection accuracy Sawo improvement Let Reproducibility means that the same measurement object can be measured using the same method regardless of the person who measures, the equipment, the measurement location, or the measurement time. home When at least one of the measurements is performed under different conditions of The measurement results may be in agreement to some extent.

[0036] The detection well 140 is provided with the detection target contained in the centrifugation well 120. It is a space where More specifically, the detection well 140 may be a space in which the detection target object contained in the centrifugation well 120 is sucked up and then discharged by the tip member 610, and the discharged detection target object is accommodated.

[0037] The detection well 140 is a cuvette so that transmission detection can be performed. of structure to haveThe cuvette may be a small, tubular container designed to hold a sample for spectroscopic measurement. The detection well 140 may be made of a transparent material so that light generated by the absorptiometer 200 (described later) can pass through the detection target accommodated in the detection well 140 to measure absorbance.

[0038] In one embodiment, the detection well 140 can be a plastic cuvette. More than accuracy It can be used for high-speed spectroscopic analysis where measurement speed is important, and can be disposed of after use. 、 For reuse twist This prevents contamination from occurring. Additionally, plastic cuvettes are made of polymethyl methacrylate (PMMA) and polystyrene (PS), which allows for lower manufacturing costs than conventional cartridges. In another embodiment, the detection well 140 may be a glass cuvette. In yet another embodiment, the detection well 140 may be a quartz cuvette. In this case, the quartz cuvette is more durable than either the plastic or glass cuvettes and transmits light of a wider wavelength range than either the plastic or glass cuvettes. Let It is possible.

[0039] The cover 150 is formed to seal the top surface of the centrifugal cartridge 100. The cover 150 seals the top surface of the centrifugal cartridge 100 to prevent the sample contained in the centrifugal cartridge 100 from being released during centrifugation. The cover 150 covers the top surface of the centrifugal cartridge 100 except for the sample well 110 into which the sample is injected before centrifugation. Part can be sealed.

[0040] The cover 150 may be made of a material that can be penetrated by the tip member 610. In one embodiment, the cover 150 is an aluminum cover formed by heat sealing. However, the present invention is not limited to this, and the cover 150 may be made of a material that can be penetrated by the tip member 610.

[0041] The tube cap 160 can open and close the sample well 110. More specifically, the tube cap 160 is disposed on the top surface of the centrifugal cartridge 100 and can open and close the sample well 110, thereby controlling the movement of materials into and out of the sample well 110. In one embodiment, the tube cap 160 is disposed on the top surface of the centrifugal cartridge 100 so that the sample is injected into the sample well 110 before centrifugation. 、 Sample well 110 and separated Alternatively, the sample well 110 may be removed and opened. In another embodiment, the tube cap 160 is coupled to the sample well 110 to prevent the sample from being removed from the sample well 110 during centrifugation. Or connect And Close sample well 110. It is possible.

[0042] The absorptiometer 200 is coupled to the detection well 140 into which the substance to be detected is injected. Or concatenation do 、 The absorptiometer 200 is a cuvette structure that can perform transmission detection of an injected detection target. to have In one embodiment, the absorptiometer 200 can measure the absorbance of the target substance by passing a light beam through the detection well 140 formed in the absorptiometer 200. (Or, the detection light emitted from the color LED 220 is focused.) Multiple LED Lenses 210 and , A plurality of detection lights having different wavelengths are emitted, each having a fixed wavelength. Multiple Color LED220 and , Each transmits only wavelengths within a certain range Multiple bandpass filters 230 and, It can contain 220 color LEDs. ShootingThe emitted detection light passes through the LED lens 210 and the band pass filter 230 in order to have a certain wavelength. Each one combination Depending on what Detected light with one wavelength (Detection light in a specific wavelength range) of Shooting Emitted light Shooting Debe teeth formation And In one embodiment, teeth , which have different wavelengths (They have different wavelength bands) Multiple light sources that emit detection light Shooting The protrusion is circular Or spaced apart along a circle, A plurality of such sensors are arranged. 、 Multiple lights Shooting From one of the departments Selected Shooting Emitted detection light (Detection light with one wavelength band) Detection can be performed using: The drive motor 240 rotates the plurality of light emitting units around the center of the circle.

[0043] In this way, multiple lights Shooting From one of the departments Selected Shooting A diagnosis of a single component is performed by detecting the detected light of a specific wavelength emitted by the light selection unit. Selected If detection light of other wavelengths is used for detection, other components can be diagnosed.

[0044] The DM filter 260 allows only light having a wavelength at which the substance to be detected absorbs light to the maximum to pass through. More specifically, the DM filter 260 reflects light having an excitation wavelength. Shooting It may be a special filter designed to pass light having an emission wavelength. The DM filter 260 may include a dichroic mirror. Also, an absorption spectrophotometer 2 00 includes a monitoring PD 270 that monitors the wavelength value of the light reflected by the DM filter 260 to detect the light. Shooting From the department Shooting be served ShootingThe luminous intensity of the emitted light can be controlled to a constant value. The monitoring PD 270 may include a photodiode.

[0045] The light passing through the DM filter 260 is reflected by the mirror 280. 、 The substance to be detected can pass through the detection well. 140 The transmitted light contains the detection PD290 , Absorption Photometer 200 The light detection unit can calculate the detection value.

[0046] The cartridge loading unit 300 is a device that houses the centrifugal cartridge 100 and the absorptiometer 200. More specifically, the cartridge loading unit 300 has a cartridge hole that is larger in size than the centrifugal cartridge 100 so that the centrifugal cartridge 100 can rotate. In addition, the cartridge loading unit 300 is used to connect the centrifugal cartridge 100 that has been centrifuged and the absorptiometer 200 to measure the absorbance of the detection target. Or connect Space have do.

[0047] The rotation driver 400 is a device installed inside the cartridge loading unit 300 and rotates, and can carry out centrifugation by mounting the centrifugal cartridge 100 thereon. In one embodiment, the rotation driver 400 has a rotational speed of 4000 rpm. of The rotation driving unit 400 can rotate at high speed to move the sample accommodated in the sample well 110 to the centrifugation well 120 through the sample moving passage 130. death The samples were divided into two groups: one for the detection target and one for the non-detection target. Part It can be separated into

[0048] In another embodiment, the rotation driver 400 may rotate stepwise. More specifically, the rotation driver 400 rotates the tip member 610 when the tip member 610 is inserted into the centrifugation well 120 and the target substance is aspirated. When Center of the tip member 610 A vertical line passing through and the center of centrifugation well 120 A vertical line passing through and are on the same line Located like 、 The rotation drive unit 400 rotates stepwise. When the tip member 610 that has sucked in the detection target substance discharges the detection target substance into the detection well 140, the rotation drive unit 400 rotates the tip member 610 so that the tip member 610 rotates stepwise. The above line passing through is the center of the detection well 140 A vertical line passing through on the same line as Location As if 、 Can rotate in steps.

[0049] The horizontal drive unit 500 is located below the cartridge loading unit 300. direction More specifically, the horizontal driving unit 500 moves the cartridge loading unit 300 in a direction parallel to the front surface (for example, the ZY plane). plane ) seen from Time , one of the two sides of the cartridge loading section 300 The other side side The horizontal driving unit 500 may be provided with a guide rail for moving the cartridge loading unit 300 in the horizontal direction (e.g., the X-axis direction or the −X-axis direction).

[0050] The guide rail G may be formed to extend parallel to the moving direction (X-axis direction) of the horizontal driving unit 500. case , a protruding portion 304 protrudes in the lateral direction (Y-axis direction) from the side end of the cartridge loading portion 300. do The guide protrusions are formed It may be In one embodiment, the guide protrusion g is provided on the side of the cartridge loading portion 300. surface Circumferential direction of the bottom end Or the direction in which the edge extends It extends along and is connected to the guide rail G. It may be .

[0051] In another embodiment, the guide protrusion g is provided on the side of the cartridge loading portion 300. surface Circumferential direction of the bottom end Or the direction in which the edge extends and extends along the side of the cartridge loading section 300. surfaceAround the bottom edge Or fate In this case, the guide rail G extends parallel to the guide protrusion g, and one surface of the guide rail G (hereinafter referred to as the guide surface) is formed on one surface of the guide protrusion g. (For example, the surface facing the Y-axis direction) As a result, when the cartridge loading portion 300 moves in the horizontal direction (X-axis direction or -X-axis direction), the guide protrusions g slide along the guide surfaces of the guide rails G, thereby preventing the cartridge loading portion 300 from tilting.

[0052] The syringe 600 is mounted on the cartridge loading section 300. Located above the centrifugal cartridge 100 The syringe 600 is positioned at the upper part of the step 121 formed at the bottom of the centrifugation well 120, and can suck the detection target and inject it into the detection well 140. More specifically, the syringe 600 is positioned at the upper part of the step 121 formed at the bottom of the centrifugation well 120. Above The sample can be inserted into the detection well 140 to suck up the sample to be detected, and the sample can be discharged into the detection well 140. case , the syringe 600 can include a tip member 610 .

[0053] The tip member 610 is disposed at the lower end of the syringe 600, and is the part through which the detection target substance is aspirated and discharged. When the detection target substance is aspirated, the tip member 610 may be in contact with the upper part of the step 121 (the shallow bottom part of the centrifugal separation well 120) as shown in FIG. The tip member 610 can penetrate the top cover 150 of the centrifugal cartridge 100 and suck up the detection target substance contained in the centrifugal well 120. case Tip member 610 may be formed with a distally decreasing diameter to penetrate cover 150 . The object to be detected was sucked in. The tip member 610 、 In the detection well 140, which is the space where the absorbance is measured, 、 The object to be detected can be ejected.

[0054] The syringe driving unit 700 can move the syringe 600. More specifically, the syringe driving unit 700 controls the syringe 600 to suck and discharge the detection target. ForIn one embodiment, the syringe driving unit 700 drives the syringe 600 to aspirate the detection target object accommodated in the centrifugation well 120. For In another embodiment, the syringe driving unit 700 drives the syringe 600 to discharge the detection target sucked into the detection well 140. For The actuator can be driven upward (in the Z-axis direction).

[0055] The housing 800 covers the centrifugal cartridge 100, the absorptiometer 200, the cartridge loading unit 300, the rotary drive unit 400, the horizontal drive unit 500, the syringe 600, and the syringe drive unit 700. (surround) The housing 800 may include a printer 810, a touch screen 820, a progress indicator 830, a power switch 840, and a door 850.

[0056] The printer 810 may be a thermal printer that applies heat to thermal paper to print characters or pictures. More specifically, the printer 810 is a thermal printer that is a non-impact printing device. The thermal printer has a recording head by It may also be a printer that uses heat to develop color on thermal paper and prints letters and pictures using a dot method. teeth However, this is not limited to this. case The printer 810 is configured to rotate the housing 800 from the front (for example, parallel to the ZY plane). plane ) seen from Time , can be disposed at the top end of the housing 800.

[0057] The touch screen 820 is used by a user to input commands to operate the centrifugal immunoassay device 10. The touch screen 820 may be a touch-type display device that inputs commands using a part of the user's body, such as a finger or palm, or a means for touching.

[0058] The touch screen 820 is used to input commands so that the centrifugal immunoassay device 10 performs a number of operations. In order to Touch buttons may be provided. In one embodiment, the touch screen 820 may include a start button and a pause button. However, the present invention is not limited to this. In another embodiment, the touch screen 820 may include a rotation operation button for performing centrifugation using the rotation driver 400, and a detection operation button for moving the centrifuged detection target to the detection well 140 and performing a detection operation.

[0059] The progress indicator 830 is located on the front side of the housing 800 (e.g., parallel to the ZY plane). plane ) facing The user can check the degree of operation of the centrifugal immunoassay device 10. Can Display it externally so that it looks like this.

[0060] The power switch 840 for example, Turn on the power of the centrifugal immunoassay device 10. on or off Here, the power switch 840 is a switch that is attached to the front surface of the housing 800 (for example, a surface parallel to the ZY plane). plane ) facing It may be placed on a surface.

[0061] The door 850 allows the user to load the centrifugal cartridge 100 into the centrifugal immunoassay device 10. Can It can be opened and closed like this. case The door 850 is located on the front side of the housing 800 (for example, parallel to the ZY plane). plane ) facing It can be placed on a surface.

[0062] The control unit can control the operation of the centrifugal immunoassay device 10 based on commands input by the user via the touch screen 820. More specifically, the control unit can control the absorptiometer 200, the rotational drive unit 400, the horizontal drive unit 500, the syringe 600, and the syringe drive unit 700 based on commands input by the user via the touch screen 820.

[0063] In one embodiment, a start button provided on the touch screen 820 is pressed. (Operate on the screen) The centrifugal immunoassay device 10 performs the following operations: centrifuging the sample by rotation of the rotary drive unit 400; moving the syringe 600 up or down by the syringe drive unit 700; and aspirating and discharging the detection target substance by the tip member 610 provided on the syringe 600. and, The centrifugal cartridge 100 and the absorptiometer 200 are combined. Or connect The absorptiometer 200, the rotational drive unit 400, the horizontal drive unit 500, the syringe 600, and the syringe drive unit 700 can be controlled so that the operation of detecting the detection target is performed.

[0064] If a problem occurs during the operation of the centrifugal immunoassay device 10, the user can press the pause button on the touch screen 820 to stop the operation. (Operate on the screen) The operation being performed by the centrifugal immunoassay device 10 can be temporarily stopped.

[0065] The centrifugal immunoassay device 10 according to the embodiment of the present invention as described above can perform the centrifugation and transmission detection in one centrifugal cartridge 100, thereby completing the detection process in a single device. Using , one centrifugal cartridge 100 Using The detection results can be confirmed while centrifugation and detection are performed, which effectively reduces the time and cost required for detection. Furthermore, by selectively using detection light of many wavelengths, multiple diagnosis is possible with a single detection system.

[0066] The above description of the present invention is for illustrative purposes only and does not change the technical idea or gist of the present invention. as long as The present invention can be easily modified into other specific forms. Therefore, the above-described embodiments are illustrative in all respects and are not limiting. For example, each component described as a single component may be implemented in a distributed manner. Opposition Components described as distributed teeth , join Or connect It can be implemented in the form described above.

[0067] All modifications and variations that come within the scope of the claims and their equivalents belong to the scope of the present invention. [Explanation of symbols]

[0068] 10: Centrifugal immunoassay device 100: Centrifugal cartridge 200: Spectrophotometer 300: Cartridge loading section 400: Rotation drive unit 500: Horizontal drive unit 600: Syringe 700: Syringe drive unit 800: Housing

Claims

1. A centrifugal immunoassay device that separates and detects the target substance from the sample by centrifugation, comprising: a centrifugal cartridge having a plurality of wells in which a sample is accommodated; a rotary drive unit on which the centrifugal cartridge is mounted and rotates; an absorptiometer that is coupled to the centrifugal cartridge and measures the absorbance of the target substance in a detection well among the plurality of wells; a syringe that sucks up the target substance and dispenses it into the detection well; and a housing that surrounds the centrifugal cartridge, the rotary drive unit, the absorptiometer, and the syringe, The absorptiometer comprises: a light emitting unit that sends only detection light in a specific wavelength range to the detection target in the detection well, and a light detecting unit that measures the detection light that has passed through the detection target in the detection well and calculates a detection value, a plurality of light emitting units are provided so as to emit a plurality of detection lights having wavelength bands in different regions from each other; a light selection unit that selects a detection light having a wavelength band of any one of a plurality of detection lights emitted from the plurality of light emission units and sends the detection light to the detection target in the detection well; A centrifugal immunoassay device characterized in that the light emitting unit includes a color LED that emits detection light of a certain wavelength, an LED lens that focuses the detection light emitted from the color LED, and a bandpass filter that passes only wavelengths in a certain range.

2. the sample is blood; 2. The centrifugal immunoassay device according to claim 1, wherein the object to be detected is blood plasma.

3. 2. The centrifugal immunoassay device according to claim 1, wherein the detection well has a cuvette structure that allows transmission detection.

4. The centrifuge cartridge may include, as a plurality of wells, in addition to the detection well: a sample well in which the sample is contained; a centrifuge well into which the sample is moved from the sample well by the rotating action, 2. The centrifugal immunoassay device according to claim 1, wherein the centrifugal cartridge further comprises a sample transfer passage that connects the sample well and the centrifugal well to form a flow path.

5. the syringe is positioned above the centrifugal cartridge; the syringe has a tip member at a lower end thereof for aspirating the detection target substance; 5. The centrifugal immunoassay device according to claim 4, wherein the tip member penetrates the upper surface of the centrifugal cartridge, aspirates the detection target contained in the centrifugal well, and ejects the aspirated detection target into the detection well.

6. a step is formed at the bottom of the centrifugation well to aspirate only the detection target separated from the sample by the centrifugation; 6. The centrifugal immunoassay device according to claim 5, wherein the tip member is inserted above the step in the centrifugal well to aspirate the detection target substance.

7. the plurality of light exit portions are arranged at intervals along a circle, the light selecting unit includes a drive motor that rotates the plurality of light emitting units that are arranged at intervals along a circle; 2. The centrifugal immunoassay device according to claim 1, further comprising: a position sensor that detects the position of the light emitting part.

8. The centrifugal immunoassay device according to claim 1, wherein the housing comprises at least one of a printer, a touch screen, a progress indicator, a power switch, and a door.

9. The centrifugal immunoassay device according to claim 8, wherein the touch screen is used by a user to input commands for operating the centrifugal immunoassay device for multiplexed diagnosis.

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