Emulsifying components

Abstract

To provide a novel emulsifying composition derived from a microorganism.SOLUTION: An emulsifying composition includes a cultured product of a microorganism of genus Cupriavidus or extract from the cultured product, where the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115), the cultured product is obtained by culturing the microorganism on a culture medium containing compounds expressed by the following formula (I) and / or formula (II). In the formula, R1 denotes a C10-20 saturated or unsaturated hydrocarbon group, R2 denotes a C9-19 saturated or unsaturated hydrocarbon group, A1 and A2 denote, each independently, ethylene group, propylene group or butylene group, and m and n denote, each independently, integers of 0 to 50.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to an emulsifying composition. [Background technology]

[0002] Surfactants are used in many industrial fields, including pharmaceuticals, cosmetics, and food. Many surfactants are industrially produced through chemical reactions using fatty acids produced by the hydrolysis of fats and oils as raw materials. However, there are also surfactants produced by living organisms. Among these surfactants, those produced extracellularly by microorganisms are called biosurfactants. Biosurfactants are generally known to have low skin irritation, easy decomposition, and high surface activity due to their low critical micelle concentration.

[0003] For example, Patent Document 1 reports a method for producing a biosurfactant from tetrachloroethylene using microorganisms in activated sludge. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2016-174537 Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a novel emulsifying composition derived from a microorganism. [Means for solving the problem]

[0006] As a result of intensive research, the present inventors have found that the above problems can be solved by the following means, and have thus completed the present invention.

[0007] A first aspect of the present invention is an emulsifying composition comprising a culture of a microorganism of the genus Cupriavidus or an extract from the culture, the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) (also referred to simply as "NNM23 strain" herein) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115) (also referred to simply as "NNM27 strain" herein), The culture is a composition for emulsification obtained by culturing the microorganism in a medium containing a compound represented by the following formula (I) and / or formula (II):

[0008] [ka]

[0009] In the formula, R 1 is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, and R 2 is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, and A 1 and A 2 are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

[0010] A second aspect of the present invention comprises culturing a microorganism of the genus Cupriavidus in a medium containing a compound represented by the following formula (I) and / or formula (II): The method for producing an emulsifying composition, wherein the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115):

[0011] [ka]

[0012] In the formula, R 1 is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, and R 2 is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, and A 1 and A 2 are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50. [Effects of the Invention]

[0013] According to the present invention, a novel emulsifying composition derived from a microorganism is provided. [Brief explanation of the drawings]

[0014] [Figure 1] 1 shows photographs showing the state of culture solutions in Examples and Comparative Examples. DETAILED DESCRIPTION OF THE INVENTION

[0015] An embodiment of the present invention will be described below, but the present invention is not limited to the following embodiment.

[0016] In this specification, the range "X to Y" means "X or more and Y or less." Unless otherwise specified, operations and measurements of physical properties are performed at room temperature (20 to 25°C) and a relative humidity of 40 to 50% RH.

[0017] <Emulsifying composition> A first aspect of the present invention is an emulsifying composition comprising a culture of a microorganism of the genus Cupriavidus or an extract from the culture, the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115), The culture is a composition for emulsification obtained by culturing the microorganism in a medium containing a compound represented by the following formula (I) and / or formula (II):

[0018] [ka]

[0019] In the formula, R 1 is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, and R 2 is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, and A 1 and A 2 are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

[0020] In the present invention, the microorganism of the genus Cupriavidus is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115).

[0021] As the microorganism according to the present invention, it is preferable to use at least the NNM27 strain.

[0022] The Cupriavidus sp. NNM23 strain was deposited at the Patent Microorganisms Depositary Center of the National Institute of Technology and Evaluation (2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture) on September 4, 2015, and its accession number is NITE P-02114.

[0023] The Cupriavidus sp. NNM27 strain was deposited at the Patent Microorganisms Depositary Center of the National Institute of Technology and Evaluation (2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture) on September 4, 2015, and its accession number is NITE P-02115.

[0024] In the present invention, the culture of a microorganism of the genus Capriavidus refers to a culture broth in which a microorganism of the genus Capriavidus has been cultured and a supernatant obtained by centrifuging the culture broth.

[0025] The culture according to the present invention is obtained by culturing the NNM23 strain and / or the NNM27 strain in a medium containing a compound represented by the following formula (I) and / or formula (II).

[0026] [ka]

[0027] In the formula, R 1 is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, and R 2 is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, and A 1 and A 2 are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

[0028] In the above formula (I), the saturated or unsaturated hydrocarbon group preferably has 10 to 16 carbon atoms, and more preferably 10 to 14 carbon atoms.

[0029] In the above formula (II), the saturated or unsaturated hydrocarbon group preferably has 13 to 19 carbon atoms, and more preferably 15 to 19 carbon atoms.

[0030] In the above formulas (I) and (II), the saturated or unsaturated hydrocarbon group may be linear or branched.

[0031] Examples of saturated hydrocarbon groups include n-nonyl, n-decyl, n-undecyl, n-dodecyl, n-tridecyl, n-tetradecyl, n-pentadecyl, n-hexadecyl, n-heptadecyl, n-octadecyl, n-nonadecyl, and n-eicosyl groups.

[0032] Examples of unsaturated hydrocarbon groups include nonenyl, decenyl, undecenyl, dodecenyl, tridecenyl, tetradecenyl, pentadecenyl, hexadecenyl, heptadecenyl, octadecenyl, nonadecenyl, and eicosenyl groups.

[0033] R 2 is a saturated hydrocarbon group, R 2 Examples of the group represented by -CO- include a decanoyl group, an undecanoyl group, a dodecanoyl group (lauroyl group), a tridecanoyl group, a tetradecanoyl group (myristoyl group), a pentadecanoyl group, a hexadecanoyl group (palmitoyl group), a heptadecanoyl group, an octadecanoyl group (stearoyl group), a nonadecanoyl group, and an eicosanoyl group.

[0034] R 2 When R is an unsaturated hydrocarbon group, 2 Examples of the group represented by -CO- include an oleoyl group, a linoleoyl group, an α-linolenoyl group, a γ-linolenoyl group, and a palmitrenoyl group.

[0035] In the above formulas (I) and (II), A 1 and A 2 is preferably an ethylene group.

[0036] In the above formula (I), when m is an integer of 2 or more, A 1 may independently be an ethylene group, a propylene group, or a butylene group.

[0037] In the above formula (II), when n is an integer of 2 or more, A 2 may independently be an ethylene group, a propylene group, or a butylene group.

[0038] In the above formula (I), m is preferably an integer of 1 to 50, more preferably an integer of 1 to 30, even more preferably an integer of 1 to 8, and particularly preferably an integer of 1 to 3.

[0039] In the above formula (II), n is preferably 0.

[0040] Examples of the compound represented by the above formula (I) include ethylene glycol monodecyl ether, diethylene glycol monodecyl ether, triethylene glycol monodecyl ether, propylene glycol monodecyl ether, butylene glycol monodecyl ether, ethylene glycol monoundecyl ether, diethylene glycol monoundecyl ether, triethylene glycol monoundecyl ether, propylene glycol monoundecyl ether, butylene glycol monoundecyl ether, ethylene glycol monododecyl ether, diethylene glycol monododecyl ether, triethylene glycol monododecyl ether, propylene glycol monododecyl ether, butylene glycol monododecyl ether, ethylene glycol monotridecyl ether, diethylene glycol monotridecyl ether, triethylene glycol monotridecyl ether, propylene glycol monotridecyl ether, butylene glycol monotridecyl ether, ethylene glycol monotetradecyl ether, diethylene glycol monotetradecyl ether, triethylene glycol monotridecyl ether, propylene glycol monotridecyl ether, butylene glycol monotridecyl ether, ethylene glycol monotetradecyl ether, Cholesterol monotetradecyl ether, propylene glycol monotetradecyl ether, butylene glycol monotetradecyl ether, ethylene glycol monopentadecyl ether, diethylene glycol monopentadecyl ether, triethylene glycol monopentadecyl ether, propylene glycol monopentadecyl ether, butylene glycol monopentadecyl ether, ethylene glycol monohexadecyl ether, diethylene glycol monohexadecyl ether, triethylene glycol monohexadecyl ether, propylene glycol monohexadecyl ether, butylene glycol monohexadecyl ether, ethylene glycol monoheptadecyl ether, diethylene glycol monoheptadecyl ether, triethylene glycol monoheptadecyl ether, propylene glycol monoheptadecyl ether, butylene glycol monoheptadecyl ether, ethylene glycol monooctadecyl ether, diethylene glycol monooctadecyl ether, triethylene glycol monooctadecyl ether, propylene glycol monooctadecyl ether,Examples of such ethers include butylene glycol monooctadecyl ether, ethylene glycol monononadecyl ether, diethylene glycol monononadecyl ether, triethylene glycol monononadecyl ether, propylene glycol monononadecyl ether, butylene glycol monononadecyl ether, ethylene glycol monoeicosyl ether, diethylene glycol monoeicosyl ether, triethylene glycol monoeicosyl ether, propylene glycol monoeicosyl ether, and butylene glycol monoeicosyl ether.

[0041] Examples of compounds represented by the above formula (II) include saturated fatty acids such as decanoic acid (capric acid), undecanoic acid, dodecanoic acid (lauric acid), tridecanoic acid, tetradecanoic acid (myristic acid), pentadecanoic acid (pentadecylic acid), hexadecanoic acid (palmitic acid), heptadecanoic acid (margaric acid), octadecanoic acid (stearic acid), nonadecanoic acid, and eicosanoic acid (arachidic acid); monounsaturated fatty acids such as myristoleic acid, palmitoleic acid, sapienic acid, oleic acid, elaidic acid, and vaccenic acid; diunsaturated fatty acids such as linoleic acid; and triunsaturated fatty acids such as α-linolenic acid, γ-linolenic acid, pinoleic acid, α-eleostearic acid, and β-eleostearic acid.

[0042] The medium used for culturing the microorganism according to the present invention is not particularly limited as long as it essentially contains the compound represented by formula (I) and / or formula (II) above and allows the microorganism to grow and proliferate. The medium used for culturing the microorganism may be a liquid medium or a solid medium. The medium is preferably a liquid medium from the viewpoint that components having an emulsifying effect can be easily recovered.

[0043] The components contained in the medium include the compounds represented by the above formula (I) and / or formula (II), nitrogen sources, inorganic salts and other components.

[0044] Specific examples of the compounds represented by formula (I) and / or formula (II) are as described above.

[0045] The compounds represented by the above formula (I) and / or formula (II) may be added alone or in the form of a mixture of two or more kinds.

[0046] For example, oils and fats such as safflower oil, olive oil, canola oil (rapeseed oil), perilla oil, coconut oil, sesame oil, rice bran oil, rice bran oil, soybean oil, corn oil, palm oil, palm kernel oil (coconut oil), sunflower oil, cottonseed oil, and peanut oil can be used as materials containing two or more compounds represented by formula (II).

[0047] In the culture of the microorganism according to the present invention, the compound represented by formula (I) and / or formula (II) corresponds to a carbon source. The medium used for culturing the microorganism may contain a carbon source other than the compound represented by formula (I) and / or formula (II), but it is preferable that the compound represented by formula (I) and / or formula (II) is used as the only carbon source (single carbon source).

[0048] The amount of the compound represented by formula (I) and / or formula (II) to be added is, for example, 0.02 to 20.0 w / v%, preferably 0.1 to 10.0 w / v%, more preferably 0.1 to 5.0 w / v%, and even more preferably 0.5 to 3.0 w / v%, relative to the total amount of the medium. When two or more compounds represented by formula (I) and / or formula (II) are used, the amount is the total amount, and in the case of the above-mentioned oils and fats, the amount is the mass thereof.

[0049] Examples of nitrogen sources include organic nitrogen sources such as meat extract, fish extract, peptone, polypeptone, tryptone, yeast extract, malt extract, soybean hydrolysate, soybean powder, casein, milk casein, casamino acids, various amino acids such as glycine, glutamic acid, and aspartic acid, corn steep liquor, and hydrolysates of other animals, plants, and microorganisms; and inorganic nitrogen sources such as ammonia, ammonium salts such as ammonium nitrate, ammonium sulfate, and ammonium chloride, nitrates such as sodium nitrate, nitrites such as sodium nitrite, and urea. One or more of the above nitrogen sources can be selected and used.

[0050] The amount of nitrogen source to be added (the total amount when two or more types are used) is not particularly limited as long as it allows the growth and proliferation of the microorganism according to the present invention.

[0051] Examples of inorganic substances include magnesium, manganese, calcium, sodium, potassium, boron, cobalt, nickel, molybdenum, copper, iron, and zinc. The form in which these inorganic substances are contained in the medium is not particularly limited. For example, for magnesium, manganese, calcium, sodium, potassium, cobalt, nickel, copper, iron, and zinc, halides such as phosphate, hydrochloride, sulfate, acetate, carbonate, and chloride may be used. Specific examples include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium chloride, calcium chloride, iron sulfate, magnesium sulfate, copper sulfate, manganese sulfate, cobalt chloride, nickel sulfate, and zinc sulfate. For boron, examples include boric acid. For molybdenum, examples include molybdenum oxide. One or more of the above inorganic substances may be selected and used.

[0052] The amount of inorganic substances to be blended (the total amount when two or more types are used) is not particularly limited as long as it allows the growth and proliferation of the microorganisms according to the present invention.

[0053] Other components include a solvent and a pH adjuster.

[0054] The solvent is usually water, and the water is not particularly limited, but examples thereof include pure water, distilled water, deionized water, and RO water.

[0055] Examples of pH adjusters include acids such as sulfuric acid, hydrochloric acid, nitric acid, acetic acid, and citric acid; and bases such as sodium hydroxide, potassium hydroxide, sodium carbonate, and potassium carbonate.

[0056] Specific examples of media that can be used in culturing the microorganisms of the present invention include media prepared by adding the compounds represented by formula (I) and / or formula (II) to the inorganic salt selective media used in the examples described below.

[0057] The method for preparing the medium is not particularly limited, and any conventionally known method can be used. For example, the medium can be prepared by dissolving the compound represented by formula (I) and / or formula (II), a nitrogen source, and an inorganic substance in a solvent (e.g., water).

[0058] The microorganisms of the present invention can be cultured by any known method, without any particular limitation, as long as they are capable of growing and multiplying. For example, the culture is carried out under aerobic conditions by shaking or aeration and stirring. The microorganisms may be cultured continuously or batchwise. The culture conditions are appropriately selected depending on the composition of the medium and the culture method, and are not particularly limited as long as they allow the microorganisms of the present invention to grow and multiply. The culture temperature is usually 25 to 37°C. The pH of the medium suitable for culture is not particularly limited, but is preferably 5 to 11, more preferably 6 to 10. The culture time is not particularly limited, and may be continued until emulsification of the culture solution is confirmed. The culture time is usually about 16 to 72 hours, preferably about 20 to 60 hours.

[0059] Prior to culturing the microorganism of the present invention, the microorganism may be pre-cultured. The pre-culture method is not particularly limited as long as it allows the microorganism of the present invention to grow and proliferate, and can be carried out by a known method. For example, the method described in the Examples can be used.

[0060] The medium used for pre-culture can be any of the above-mentioned media. Carbon sources contained in the medium can be those other than the compounds represented by formula (I) and formula (II). Examples of such carbon sources include organic acids and salts thereof, such as gluconic acid, capric acid, adipic acid, malic acid, citric acid, phenyl acetate, acetic acid, lactic acid, succinic acid, glucuronic acid, and pyruvic acid; hydrocarbons, such as hexadecane; natural products, such as wheat and rice; alcohols, such as glycerol, methanol, and ethanol; sugars; tryptone; and triethylene glycol monobutyl ether.

[0061] The pre-culture conditions can be appropriately determined by referring to the above culture conditions.

[0062] In this manner, a culture solution as the culture according to the present invention can be obtained.

[0063] The resulting culture solution can be centrifuged to precipitate the bacterial cells, and the supernatant can be collected. The collected supernatant contains the emulsifying component produced by the microorganism of the present invention, and can therefore be used as the culture of the present invention. The conditions for centrifugation are not particularly limited, and for example, centrifugation can be performed at 3,000 to 5,000 rpm for 3 to 10 minutes.

[0064] In the present invention, the extract from a culture of a microorganism of the genus Capriavidus means an extract obtained by solvent extraction of the culture, a diluted solution and a concentrated solution thereof, and a dried product thereof.

[0065] The method for extracting the extract from the culture is not particularly limited, and any conventionally known method can be used. For example, the extract can be obtained by the following method: (Step 1) Mixing the culture with a solvent (e.g., ethyl acetate) to obtain a mixture; (Step 2) Centrifuge the mixture (e.g., 600 rpm, 10 minutes); (Step 3) The solvent layer is recovered, and the solvent is removed from the solvent layer using a rotary evaporator or the like to obtain an extract (extract).

[0066] The solvent used for extraction is not particularly limited, and conventionally known solvents can be used. Examples of solvents include water; alcohols such as methanol, ethanol, propanol, and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; linear and cyclic ethers such as tetrahydrofuran and diethyl ether; polyethers such as polyethylene glycol; hydrocarbons such as hexane, cyclohexane, and petroleum ether; aromatic hydrocarbons such as benzene and toluene; and pyridines. The solvent is preferably an ester such as ethyl acetate or an alcohol such as ethanol.

[0067] The amount of solvent to be added varies depending on the solvent used, but is, for example, 0.5 to 10 parts by volume per 1 part by volume of culture medium. The temperature during mixing is not particularly limited, and is, for example, 25 to 37°C.

[0068] The extract obtained in step 3 can be used as is as an extract. Alternatively, the extract can be diluted, concentrated, or freeze-dried by a conventionally known method and then used as an extract.

[0069] <Method of manufacturing the emulsification composition> A second aspect of the present invention comprises culturing a microorganism of the genus Cupriavidus in a medium containing a compound represented by the following formula (I) and / or formula (II): The method for producing an emulsifying composition, wherein the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115):

[0070] [ka]

[0071] In the formula, R 1 is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, and R 2 is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, and A 1 and A 2 are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

[0072] In the method for producing an emulsification composition according to the present invention, the microorganisms (the NNM23 strain and the NNM27 strain), the compounds represented by formula (I) and formula (II), the medium used for culturing, the method for culturing the microorganisms, and the culture and extract thereof obtained by culturing are the same as those described above, and therefore further description thereof will be omitted.

[0073] In a preferred embodiment, the method for producing a composition for emulsification according to the present invention comprises removing microorganisms from a culture obtained by culturing in order to obtain the extract described above.

[0074] The method for removing microorganisms is not particularly limited, and includes conventionally known methods. For example, a method in which the culture solution is centrifuged to precipitate the bacterial cells and recover the supernatant is included. The centrifugation conditions are as described above.

[0075] Alternatively, the microorganisms can be removed by extracting an extract from the culture, as described above.

[0076] <Application> The emulsifying composition according to the present invention contains an emulsifying component derived from a microorganism, and therefore can be used in the fields of cosmetics (for example, lotions, emulsions, creams, etc.), detergents, etc. Therefore, one embodiment of the present invention is a cosmetic comprising the emulsifying composition according to the present invention. Another embodiment of the present invention is a detergent comprising the emulsifying composition according to the present invention. [Example]

[0077] The present invention will be described in more detail using the following examples and comparative examples. However, the technical scope of the present invention is not limited to the following examples. Unless otherwise specified, "%" and "parts" mean "% by mass" and "parts by mass," respectively. In the following examples, unless otherwise specified, the operations were carried out under the conditions of room temperature (20 to 25°C) and relative humidity of 40 to 50% RH.

[0078] [Preparation of preculture solution] Cupriavidus sp. NNM27 strain was inoculated onto solid medium (rich agar medium) from a frozen glycerol stock solution using a platinum loop and cultured at 30°C.

[0079] The solid medium was prepared by dissolving each component except agar in pure water to obtain the composition shown in Table 1 below, adding agar to a final concentration of 0.8 w / v%, sterilizing at high temperature and high pressure, and then dispensing the mixture appropriately and solidifying it.

[0080] [Table 1]

[0081] After confirming growth on the solid medium, the colonies on the solid medium were inoculated into a liquid medium (an inorganic salt selective culture solution containing 0.5 w / v% of a single carbon source (triethylene glycol butyl ether (BTG))) and cultured under aerobic conditions at 30°C for 12 to 20 hours (shaking speed: 40 rpm) to prepare a preculture solution.

[0082] The liquid medium was prepared by dissolving each component in pure water to obtain the composition shown in Table 2 below to prepare an inorganic salt selective culture medium, and then adding BTG to the inorganic salt selective culture medium to a final concentration of 0.5 w / v%, followed by sterilization at high temperature and high pressure.

[0083] [Table 2]

[0084] [Example 1] Two milliliters of the preculture solution was centrifuged (3,000 rpm, 5 minutes) to collect the bacterial cells. One liter of liquid medium (inorganic salt selective culture solution containing 0.5 w / v% of a single carbon source (diethylene glycol monododecyl ether (DDG))) was added to the collected bacterial cells, and the cells were cultured under aerobic conditions at 30°C with shaking (shaking speed: 40 rpm).

[0085] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not confirmed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactant produced by the NNM27 strain.

[0086] [Example 2] Cultivation was carried out in the same manner as in Example 1, except that the concentration of the sole carbon source (DDG) was 0.1 w / v %.

[0087] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked, and partial emulsification was confirmed in the upper part of the culture medium (Figure 1: Culture medium after approximately 30 hours of culturing). Because emulsification was not confirmed before culturing and the liquid medium used for culturing does not contain any components with emulsifying properties, it is believed that the emulsification of the culture medium is due to the biosurfactant produced by the NNM27 strain.

[0088] [Example 3] Cultivation was carried out in the same manner as in Example 1, except that safflower oil was used instead of DDG as the sole carbon source.

[0089] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not confirmed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactant produced by the NNM27 strain.

[0090] [Example 4] Cultivation was carried out in the same manner as in Example 1, except that olive oil was used instead of DDG as the sole carbon source.

[0091] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not confirmed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactant produced by the NNM27 strain.

[0092] [Example 5] Cultivation was carried out in the same manner as in Example 1, except that canola oil was used instead of DDG as the sole carbon source.

[0093] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not confirmed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactant produced by the NNM27 strain.

[0094] [Comparative Example 1] Cultivation was carried out in the same manner as in Example 1, except that BTG was used instead of DDG as the sole carbon source.

[0095] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked, and growth of the NNM27 strain was confirmed, but emulsification of the culture medium was not confirmed (Figure 1). Furthermore, emulsification was not confirmed before culturing either.

[0096] Comparative Example 2 Each component was dissolved in pure water to obtain the composition shown in Table 2 above, and then sterilized at high temperature and high pressure to prepare an inorganic salt selective culture medium.

[0097] 2 mL of the preculture solution was centrifuged (3000 rpm, 5 minutes) to collect the bacterial cells. 1 L of inorganic salt selective culture solution was added to the collected bacterial cells, and the cells were cultured under aerobic conditions at 30°C with shaking (shaking speed: 40 rpm).

[0098] After culturing for approximately 20 to 60 hours, the state of the culture medium was checked, but no growth of the NNM27 strain or emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Furthermore, emulsification was not confirmed before culturing either.

Claims

1. 1. An emulsifying composition comprising a culture of a microorganism of the genus Cupriavidus or an extract from the culture, the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and / or Cupriavidus sp. NNM27 strain (accession number NITE P-02115); The culture is obtained by culturing the microorganism in a medium containing diethylene glycol monododecyl ether.

2. A cosmetic comprising the emulsifying composition according to claim 1.

3. A detergent comprising the emulsifying composition according to claim 1.

4. The method comprises culturing a microorganism of the genus Cupriavidus in a medium containing diethylene glycol monododecyl ether, The method for producing an emulsification composition, wherein the microorganism is Cupriavidus sp. strain NNM23 (accession number NITE P-02114) and / or Cupriavidus sp. strain NNM27 (accession number NITE P-02115).

5. The method according to claim 4, further comprising removing the microorganism from the culture obtained by the culturing.

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