Melanin production promoter, and composition for preventing gray hair or composition for reducing gray hair containing said promoter

Abstract

To provide a melanogenesis promoter that contains a combination of plural components effective against white hair, synergistically promoting melanogenesis, and a composition for preventing white hair or a composition for improving white hair, containing the promoter.SOLUTION: A melanogenesis promoter contains component A: acetyl hexapeptide-1, component B: Angelica keiskei extract and component C: hematin. There is also provided a composition for preventing white hair or a composition for improving white hair, containing the promoter.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a novel melanin production promoter, and a composition for preventing hair graying or a composition for reducing hair graying containing said promoter. [Background technology]

[0002] There are various possible factors that contribute to the mechanism of gray hair development, but it is believed that the main factors involved are a decrease in the melanin production ability of melanocytes, a decrease in the number of melanocytes, and a decrease in the transfer of melanin to hair matrix cells.In recent years, various research into these factors has been actively conducted.

[0003] However, in reality, the only solution available is to dye gray hair (to hide gray hair) using hair dyes or color treatments, and no fundamental prevention or improvement of gray hair has been achieved. Previously, studies have been conducted to fundamentally suppress the occurrence of gray hair by applying it directly to the skin, for example, proposing that applying cosmetics containing Angelica keiskei extract directly to the skin would have a gray hair prevention effect (Patent Document 1). Furthermore, studies have been conducted to prevent and improve gray hair by promoting melanin production, for example, proposing that administering acetyl hexapeptide-1 to melanocytes would promote melanin production and have a gray hair prevention and improvement effect (Patent Document 2).

[0004] However, as mentioned above, various factors are involved in the mechanism of gray hair occurrence, and although some products can be effective in preventing gray hair to a certain extent, they are not fully satisfactory in fundamentally suppressing the occurrence of gray hair. Therefore, there has been a demand for the development of a drug that has a significantly superior effect in preventing and improving gray hair. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Publication No. 62-077307 [Patent Document 2] Japanese Patent Application Laid-Open No. 2013-014567 Summary of the Invention [Problem to be solved by the invention]

[0006] Therefore, an object of the present invention is to provide a melanin production promoter that synergistically promotes melanin production, and a composition for preventing graying or a composition for reducing graying containing the promoter.

[0007] In light of these circumstances, the present inventors have sought a method for reducing gray hair through extensive efforts, and as a result have discovered that the combined use of three components, component (A): acetyl hexapeptide-1, component (B): Angelica keiskei extract, and component (C): hematin, can synergistically enhance the ability to promote melanin production compared to when each component is used alone, leading to the invention of this invention. That is, the present invention is as follows. [Means for solving the problem]

[0008] The present invention provides a melanin production enhancer characterized by containing the following components A, B, and C: Component A: Acetyl hexapeptide-1 Ingredient B: Angelica keiskei extract Component C: Hematin

[0009] The present invention also provides a composition for preventing gray hair or a composition for reducing gray hair, which comprises the above-mentioned melanin production promoter. [Effects of the Invention]

[0010] The melanin production promoter of the present invention exhibits a significantly superior effect in that the combined use of three components, component A: acetyl hexapeptide-1, component B: Angelica keiskei extract, and component C: hematin, can synergistically enhance the melanin production-promoting ability compared to when each component is used alone. Furthermore, a composition for preventing hair graying or a composition for reducing hair graying containing the promoter exhibits the effect of being able to effectively prevent and reduce graying. [Brief explanation of the drawings]

[0011] [Figure 1] 1 is a graph showing the melanin production promotion rates of the Control, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Example 1. DETAILED DESCRIPTION OF THE INVENTION

[0012] Hereinafter, an embodiment of the present invention will be described.

[0013] The melanin production enhancer of the present invention contains acetyl hexapeptide-1, Angelica keiskei extract, and hematin. In this specification, acetyl hexapeptide-1 will be referred to as "component (A)," Angelica keiskei extract will be referred to as "component (B)," and hematin will be referred to as "component (C)."

[0014] In the present invention, "promoting melanin production" means promoting melanin production in cells that produce melanin, and "melanin production promoter" refers to a drug that promotes melanin production in cells that produce melanin.

[0015] The melanin-forming cells include, for example, melanin-forming cells derived from either humans or non-human animals, and either type of cells may be used in the present invention.

[0016] The melanin production enhancer of the present invention contains at least the above component (A), component (B) and component (C).

[0017] The composition for preventing graying or the composition for reducing graying of hair of the present invention contains a melanin production promoter.

[0018] The components (A), (B) and (C) which are essential components of the present invention will be described below.

[0019] [Component (A)] Component (A) is acetyl hexapeptide-1. Component (A) is a reaction product of a synthetic peptide consisting of six amino acids, alanine, arginine, histidine, leucine, phenylalanine, and tryptophan, with acetic acid.

[0020] The above-mentioned ingredient (A) is a compound represented by the INCI name (International Cosmetic Ingredient Dictionary and Handbook, 15th Edition, Vol. 1, 2014, p. 29): ACETYL HEXAPEPTIDE-1.

[0021] The component (A) may be a single ingredient or a mixture of ingredients with other ingredients, and is not particularly limited as long as it exhibits the desired effect, and commercially available products may be used. Specific examples of commercially available products include those under the trade name "Melitan GL" (manufactured by Lucas Meyer Cosmetics, Inc.).

[0022] The content of component (A) in the melanin production enhancer of the present invention is not particularly limited as long as it can fully exert the desired effect, but from the viewpoint of promoting melanin production, it is preferably 0.00000001% by mass or more, and more preferably 0.0000001% by mass or more, based on 100% by mass of the agent. Furthermore, from the viewpoint of safety, it is preferably 5.0% by mass or less, and more preferably 3.0% by mass or less, based on 100% by mass of the agent. The content of component (A) is the amount converted to a pure content.

[0023] By combining the above-mentioned component (A) with the components (B) and (C) described below, it is possible to achieve a significantly superior effect of synergistically enhancing the ability to promote melanin production compared to when either component is used alone.

[0024] [Component (B)] Component (B) is Angelica keiskei extract. Component (B) is a plant of the Apiaceae family, commonly found on Hachijo Island and from the Izu Peninsula to the Izu Seven Islands in Japan, and is easily available from these areas. Parts that can be used as extraction raw materials include, for example, leaves, stems, roots, flowers, seeds, the whole plant, or a mixture of these parts. In the present invention, leaves and stems are preferably used.

[0025] The above-mentioned ingredient (B) is a compound represented by the INCI name (International Cosmetic Ingredient Dictionary and Handbook, 15th Edition, Vol. 1, 2014, p. 227): ANGELICA KEISKEI EXTRACT.

[0026] Component (B) can be obtained, for example, by extracting crushed Angelica keiskei with a solvent. The extraction solvent can be, for example, water, a lower alcohol, a polyhydric alcohol, or a mixture thereof. Examples of lower alcohols include ethanol, butanol, and propanol. Examples of polyhydric alcohols include 1,3-butylene glycol and glycerin.

[0027] The Angelica keiskei extract of component (B) may be used as is, or may be filtered and concentrated as necessary, or fractionated and purified by column chromatography, etc. It may also be used after being dried under reduced pressure or freeze-dried and then prepared into a powder or paste.

[0028] The component (B) may be a single ingredient or a mixture of ingredients with other ingredients, and is not particularly limited as long as it exhibits the desired effect. A commercially available product may be used. Specific examples of commercially available products include the product name "Waism <Ashitaba>" (manufactured by Maruzen Pharmaceutical Co., Ltd.).

[0029] The content of component (B) in the melanin production enhancer of the present invention is not particularly limited as long as it can fully exert the desired effect, but from the viewpoint of promoting melanin production, it is preferably 0.00001% by mass or more, more preferably 0.0001% by mass or more, based on 100% by mass of the agent. Furthermore, from the viewpoint of safety, it is preferably 5.0% by mass or less, more preferably 3.0% by mass or less, based on 100% by mass of the agent. The content of component (B) is the amount of solid content excluding the extraction solvent.

[0030] When the above component (B) is combined with the above component (A) and the component (C) described below, it is possible to achieve a significantly superior effect of synergistically enhancing the ability to promote melanin production compared to when either component is used alone.

[0031] [Component (C)] Component (C) is hematin. Component (C) is a component produced from porcine hemoglobin. There are no particular limitations on the production method, and various known production methods can be used.

[0032] The above-mentioned ingredient (C) is a compound represented by the INCI name (International Cosmetic Ingredient Dictionary and Handbook, 15th Edition, Vol. 1, 2014, p. 1470): HEMATIN.

[0033] The component (C) may be a single ingredient or a mixture of ingredients with other ingredients, and is not particularly limited as long as it exhibits the desired effect, and a commercially available product may be used. Specific examples of commercially available products include "Glossfilin P (PF)" (manufactured by Ichimaru Pharcos Co., Ltd.).

[0034] The content of component (C) in the melanin production enhancer of the present invention is not particularly limited as long as it can fully exert the desired effect, but from the viewpoint of promoting melanin production, it is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, based on 100% by mass of the agent. Furthermore, from the viewpoint of safety, it is preferably 5.0% by mass or less, more preferably 3.0% by mass or less, based on 100% by mass of the agent. The content of component (C) is the amount converted to a pure content.

[0035] By combining the above component (C) with the above component (A) and the above component (B), it is possible to achieve a significantly superior effect of synergistically enhancing the ability to promote melanin production compared to when either component is used alone.

[0036] Since the melanin production promoter of the present invention can synergistically enhance the melanin production promoting ability, it is preferable to contain the above-mentioned component (A), the above-mentioned component (B), and the above-mentioned component (C) as active ingredients of the melanin production promoter.

[0037] According to the present invention, the accelerator that exhibits a particularly remarkable accelerating effect on melanin production can be incorporated into a composition for preventing gray hair, which leads to the prevention of gray hair, and can also be incorporated into a composition for reducing gray hair, which is effective against gray hair.

[0038] The melanin production promoter of the present invention is applied to the scalp and absorbed transdermally, promoting melanin production in melanocytes. That is, the composition for preventing hair graying or the composition for reducing hair graying of the present invention containing the production promoter promotes melanin production by the melanin production promoter, making it possible for the composition to effectively act on gray hair.

[0039] The composition for preventing gray hair or the composition for reducing gray hair of the present invention is not limited in its category and is widely used in cosmetics such as scalp lotions (scalp care), shampoos, treatments, hair growth and hair care agents, quasi-drugs, pharmaceuticals, etc.

[0040] The formulation of the composition for preventing gray hair or the composition for reducing gray hair of the present invention can be prepared and used in various forms, such as liquid, cream, emulsion, mist, gel, wax, foam, etc. Furthermore, as long as the effects of the present invention are achieved, other additives such as cationic surfactants, anionic surfactants, amphoteric surfactants, nonionic surfactants, cationic polymers, anionic polymers, amphoteric polymers, nonionic polymers, silicones, polyhydric alcohols, lower alcohols, sugar alcohols, fragrances, preservatives, plant extracts, pH adjusters, thickeners, amino acids, etc. may also be included.

[0041] The content of the melanin production promoter in the composition for preventing gray hair or the composition for reducing gray hair of the present invention is adjusted appropriately depending on the mode of use, but it is preferably incorporated in the range of 1.0 to 8.0 mass %, and more preferably 2.0 to 6.0 mass %, based on 100 mass % of the composition. [Example]

[0042] The present invention will be described in more detail below based on examples, but the present invention is not limited to these examples.

[0043] To confirm the melanin production promoting effect described below, the minimum concentration at which the melanin production promoting effect could be confirmed when each component was used alone was adopted, and samples prepared at that concentration were subjected to the test, in order to more clearly determine the effect of single use, the additive effect of combination, and the synergistic effect of combination.

[0044] [Sample 1: Preparation of medium containing "acetyl hexapeptide-1"] Acetyl hexapeptide-1 (trade name: Melitan GL, manufactured by Lucas Meyer Cosmetics, Inc.) was added to MEM medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (manufactured by BioWest) to a concentration (pure matter equivalent): 0.0000002 mass% to prepare a sample medium.

[0045] [Sample 2: Preparation of medium containing Angelica keiskei extract] A sample medium was prepared by adding Angelica keiskei extract (trade name: Washism (Ashitaba), Maruzen Pharmaceutical Co., Ltd.) to a concentration (pure matter equivalent): 0.0005% by mass to a MEM medium (Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (BioWest).

[0046] [Sample 3: Preparation of medium containing "hematin"] A sample medium was prepared by adding hematin (trade name: Glosfilin P (PF), Ichimaru Pharcos) to a concentration (pure matter equivalent): 0.001% by mass to MEM medium (Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (BioWest Co., Ltd.).

[0047] [Sample 4: Preparation of medium containing "acetyl hexapeptide-1" and "Angelica angelica extract"] Acetyl hexapeptide-1 (trade name: Melitan GL, manufactured by Lucas Meyer Cosmetics, Inc.) was added to a concentration (based on pure content): 0.0000002% by mass, and Angelica keiskei extract (trade name: Wakuism (Ashitaba), manufactured by Maruzen Pharmaceutical Co., Ltd.) was added to a MEM medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (manufactured by BioWest) to a concentration (based on pure content): 0.0000002% by mass, to prepare a sample medium.

[0048] [Sample 5: Preparation of medium containing "Ashitaba extract" and "Hematin"] A sample medium was prepared by adding angelica tree extract (trade name: Washism (Ashitaba), Maruzen Pharmaceutical Co., Ltd.) to a concentration (pure matter equivalent): 0.0005% by mass and hematin (trade name: Glossfilin P (PF), Ichimaru Pharcos Co., Ltd.) to a concentration (pure matter equivalent): 0.001% by mass to MEM medium (Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (BioWest Co., Ltd.).

[0049] [Sample 6: Preparation of medium containing "acetyl hexapeptide-1," "Angelica angelica extract," and "hematin"] Acetyl hexapeptide-1 (trade name: Melitan GL, manufactured by Lucas Meyer Cosmetics, Inc.) was added to a concentration (pure matter equivalent): 0.0000002% by mass, Angelica keiskei extract (trade name: Washism (Ashitaba), manufactured by Maruzen Pharmaceutical Co., Ltd.) was added to a concentration (pure matter equivalent): 0.0005% by mass, and hematin (trade name: Glossfilin P (PF), manufactured by Ichimaru Pharcos Co., Ltd.) was added to a MEM medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (manufactured by BioWest Co., Ltd.) to a concentration (pure matter equivalent): 0.001% by mass, to prepare a sample medium.

[0050] [Preparation of negative control] MEM medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (manufactured by BioWest Co., Ltd.) without sample was used as a negative control medium (control).

[0051] "Test Example 1: Measurement of intracellular melanin amount" MEM medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 5% FCS (manufactured by BioWest Co., Ltd.) was used as the culture medium. 2 4.0 × 10 cells were placed in a culture flask (IWAKI). 5 Mouse melanoma cells (B16 cells) were seeded at 100 cells / flask and incubated at 37°C with 5% CO 2 After that, the culture medium was replaced with the respective sample medium and negative control medium, and the cells were cultured at 37°C, 5% CO for another 3 days. 2 The cells were cultured under the conditions.

[0052] After culturing, the cells were washed with PBS(-), detached by adding trypsin-EDTA solution, and collected in a 15 mL centrifuge tube (IWAKI) and centrifuged at 1,500 rpm for 10 minutes. After discarding the supernatant, the precipitate (cell mass) was washed with PBS(-), transferred to a Spitz tube (Nipro), and centrifuged again at 1,500 rpm for 20 minutes. The supernatant was discarded, and 500 μL of a 1 M NaOH-10% DMSO mixture was added to the cell mass to extract intracellular melanin pigment.

[0053] The amount of melanin in the melanin pigment extract was measured at 490 nm using an absorbance plate reader (Thermo). The protein content of the extract was then quantified using a protein assay BCA kit (Fujifilm Wako Pure Chemical Industries, Ltd.). Specifically, the kit reaction solution was measured for absorbance at 562 nm using an absorbance plate reader (Thermo), and the melanin production promotion rate was calculated from the obtained absorbance using the following formula. The results are shown in Table 1. A graph corresponding to Table 1 is also shown in Figure 1.

[0054] <Melanin production promotion rate> (1) Amount of melanin per protein = absorbance of melanin (490 nm) / absorbance by BCA kit (562 nm) (2) Melanin production promotion rate (%) = Amount of melanin per protein in sample / Amount of melanin per protein in control (no sample added) × 100

[0055] [Table 1]

[0056] In Table 1, "+" indicates that the ingredient was added, and "-" indicates that the ingredient was not added.

[0057] As is clear from the results in Table 1 (Figure 1), it was confirmed that the combined use of acetyl hexapeptide-1, Angelica keiskei extract, and hematin synergistically promoted melanin production compared to when each was used alone, demonstrating that this was not simply an additive effect. Therefore, it can be said that the melanin production promoter of the present invention is useful for preventing or improving gray hair.

[0058] [Preparation of Composition 1] Scalp lotions of Example 2 and Comparative Example 6 were prepared in a standard manner according to the compositions shown in Table 2 below, and were subjected to the following evaluations. The results are shown in Table 2. Note that the blend amounts in the table are all values ​​converted into pure components, and are expressed in "% by mass" unless otherwise specified.

[0059] (Evaluation of gray hair improvement) The scalp lotions of Example 2 and Comparative Example 6 were used by five healthy individuals (aged 35 to 50) who were concerned about gray hair. 1 ml of each lotion was applied evenly to half of the scalp twice a day (after washing hair at night and before styling in the morning) for nine months. Specifically, the scalp lotion of Example 2 was applied to the right half of the head, and the scalp lotion of Comparative Example 6 was applied to the remaining left half of the head.

[0060] The condition of the application site was compared before and after the test to evaluate the effect of improving gray hair. The evaluation was carried out by visually evaluating "improvement of gray hair" and scoring according to the following evaluation points of 1 to 5. The evaluation was determined by comprehensively judging the individual evaluations of the five people who used the product. The evaluation of "improvement of gray hair" was given a higher score the more effective it was at improving gray hair.

[0061] The evaluation was carried out according to the following five-point rating scale. The results were calculated by calculating the average score of the five people who performed the evaluation, and the evaluation was carried out according to the following rating scale.

[0062] <Evaluation criteria> 5 points: Very good 4 points: good 3 points: normal 2 points: Defective 1 point: Very poor

[0063] <Judgment criteria> ◎: Average 4.0 points or more ○: Average 3.0 points or more and less than 4.0 points △: Average 2.0 points or more and less than 3.0 points ×: Average score less than 2.0

[0064] [Table 2]

[0065] As is clear from the results in Table 2, the scalp lotion containing the melanin production promoter of Example 2 has a greater effect on preventing gray hair than the scalp lotion not containing a melanin production promoter (Comparative Example 6). In addition, since the composition containing the melanin production promoter of the present invention has a gray hair prevention effect when applied to gray hair, it can be said that it also has a gray hair prevention effect when applied to black hair.

[0066] Hereinafter, one embodiment of a composition for preventing gray hair or a composition for reducing gray hair containing the melanin production promoter of the present invention will be described. The contents are in mass %. The preparation method is a conventional method.

[0067] Example 3: Scalp lotion (mass%) BG 9.0 Glycerin 3.0 Methylparaben 0.1 Melanin production promoter 4.0 Purified water remainder Total 100.0

[0068] Example 4: Shampoo (mass%) Sodium Laureth Sulfate 8.0 Lauramidopropyl Betaine 2.5 Decyl glucoside 4.0 Sodium chloride 0.1 Methylparaben 0.2 Glycerin 1.0 Fragrance 0.2 Melanin production promoter 4.0 Purified water remainder Total 100.0

[0069] Example 5: Hair Treatment (mass%) Dicocodimonium chloride 0.5 Steartrimonium Chloride 2.0 Cetyl alcohol 6.0 Mineral oil 1.0 Dimethicone 4.0 Glycerin 1.0 Fragrance 0.2 Melanin production promoter 4.0 Purified water remainder Total 100.0

Claims

1. A melanin production promoter comprising the following components A, B, and C: Component A: Acetyl hexapeptide-1 Ingredient B: Angelica keiskei extract Component C: Hematin

2. A composition for preventing gray hair or a composition for reducing gray hair, comprising the melanin production promoter according to claim 1.

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