Consumable parts in fluid sample distribution systems and methods

Abstract

To disclose a system, a device and a method for dispensing a fluidic sample to a plurality of targets.SOLUTION: A sample dispenser includes: an inlet port for inputting a sample material; and a first sample nozzle and a second sample nozzle that are fluidically coupled to the inlet port for discharging the sample material, in which a tubular junction part fluidically couples the inlet port to a first tubular fluidic path terminating in the first sample nozzle, and fluidically couples the inlet port to a second tubular fluidic path terminating in the second sample nozzle. A cross-sectional area of the tubular junction part is less than an average cross-sectional area of the first tubular fluidic path and the second tubular fluidic path, and the cross-sectional area of the tubular junction part is selected so as to draw the sample material from the inlet port into the tubular junction part via a capillary action.SELECTED DRAWING: Figure 1A

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application is a continuation of U.S. Provisional Patent Application No. 62 / 830,294, filed April 5, 2019. This application claims priority from the International Application Publication No. 2006 / 010999, filed on May 1, 2006, the entire contents of which are incorporated herein by reference. Rely on.

[0002] The present disclosure relates to systems, devices, and methods for dispensing a predetermined volume of a fluid sample. Concerning consumable parts in [Background technology]

[0003] Cytology techniques have evolved to be minimally invasive and have revolutionized the practice of medicine. This procedure is generally preferred because it allows for rapid, high-quality samples to be obtained without the need for additional More recently, sample collection techniques have finally become more acceptable. Employing consumables designed to improve laboratory practices while providing patient care The use of consumable parts reduces cross-contamination and improves reliability. Summary of the Invention

[0004] SYSTEM FOR DISTRIBUTION OF SUBSTANTIALLY EQUAL VOLUMES OF A FLUID SAMPLE TO EACH OF A Plurality OF TARGETS, In one exemplary embodiment, a consumable part in a device and method is disclosed. Sample dispenser for distributing substantially equal volumes of liquid-based sample material to multiple targets A dispenser, the dispenser having a first interior volume and a first inner perimeter. a first tubular structure, (i) a first opening of the first tubular structure, the first opening including a first gas nozzle and a second gas nozzle; A first gas nozzle is disposed between the first target and the second target, and directs gas toward the first target. (ii) the first periphery is configured to discharge the first periphery through a second opening in the first tubular structure. and inducing capillary action to fill the first tubular structure with a liquid-based sample material. a first tubular structure having a second internal volume and a second inner circumference, the first tubular structure being selected to be able to a second tubular structure for receiving a second gas nozzle; The second gas nozzle is disposed between the nozzle and the second target, and the second gas nozzle is directed toward the second target. (ii) the second periphery is configured to vent gas through a second opening in the second tubular structure; Inducing capillary action to fill the second tubular structure with a liquid-based sample material via the a second tubular structure selected to be able to circulate the first internal volume and the second The internal volumes are substantially the same, and the second opening of the first tubular structure is connected to the second opening of the second tubular structure. A dispenser in close proximity to the opening.

[0005] In another exemplary embodiment, a system for analyzing a sample includes: a first gas nozzle configured to eject gas toward a second target; a second gas nozzle configured to discharge a gas; and a nozzle configured to hold the first and second targets. The target holder is arranged to hold multiple targets and to hold the targets in a substantially equal volume of liquid. a sample dispenser for dispensing the body-based sample material, the dispenser comprising: a first tubular structure having a first interior volume and a first inner circumference, the first tubular structure comprising: The first opening of the structure is disposed between the first gas nozzle and the first target, and (ii) ) the first periphery is filled with a liquid-based sample material through a second opening in the first tubular structure; a first electrode selected to be capable of inducing capillary action to fill the tubular structure of one electrode; a tubular structure; and a second tubular structure having a second interior volume and a second inner circumference, The first opening of the second tubular structure is disposed between the second gas nozzle and the second target. (ii) a second periphery is provided with a liquid-based sample through a second opening in the second tubular structure; The second tubular structure is filled with a capillary material selected to induce capillary action. and a second tubular structure, the first internal volume and the second internal volume being substantially the same. and the second opening of the first tubular structure is adjacent to the second opening of the second tubular structure. Stem.

[0006] In yet another exemplary embodiment, an apparatus for dispensing a fluid sample includes: The inlet port is used to input the sample material, and the inlet port is used to output the sample material. a first sample nozzle and a second sample nozzle connected together, the tubular junction being a first tubular fluid path terminating in a first sample nozzle; a second tubular fluid path terminating in a nozzle, the cross-sectional area of ​​the tubular junction being equal to or larger than the first a cross-sectional area of ​​the tubular junction that is smaller than the average cross-sectional area of ​​the tubular fluid path and the second tubular fluid path; is selected to draw sample material from the inlet port into the tubular junction via capillary action. The device to be used.

[0007] In yet another exemplary embodiment, a sample analysis system includes: 1. A hood for improving deposition of sample material, the hood comprising: a top panel; a front panel; , a main body having a left panel, a right panel, and a rear panel, and a top panel for storing samples. a first opening configured to receive a material, the first opening being parallel to the top panel and in the back panel; a vertical lower panel positioned adjacent to the rear panel, the lower panel a second opening for receiving a dye or colorant after deposition of the sample material on the get; and a bottom rim supporting the body and the lower panel, the bottom rim being attached to the target prior to deposition. A hood configured to adhere to the

[0008] These and other aspects and implementations thereof are set forth in the drawings, specification, and claims. The scope is explained in more detail below. [Brief explanation of the drawings]

[0009] [Figure 1A] 1 illustrates an example of a sample analysis system.

[0010] [Figure 1B] 1 illustrates another example of a sample analysis system.

[0011] [Figure 2] FIG. 1B shows an example of a sample dispenser.

[0012] [Figure 3] 1B shows another example of the sample dispenser of FIG. 1A.

[0013] [Figure 4] 1B shows yet another example of the sample dispenser of FIG. 1A.

[0014] [Figure 5A-B] An example of a sample dispenser is shown.

[0015] [Figure 6A-B] 1 shows another example of a sample dispenser.

[0016] [Figure 7A-B] 10 shows yet another example of a sample dispenser.

[0017] [Figure 8] 6A and 6B show an example of spraying a sample using the sample dispenser shown in FIG. 6B.

[0018] [Figure 9A] 1 shows an example of an inlet port on a sample dispenser.

[0019] [Figure 9B] 9B illustrates the geometry of the inlet port of FIG. 9A.

[0020] [Figure 10A] 10 shows another example of an inlet port on a sample dispenser.

[0021] [Figure 10B] 10B illustrates the geometry of the inlet port of FIG. 10A.

[0022] [Figure 11] 1 shows an example of a tubular structure connecting the inlet port of the sample dispenser with the sample nozzle.

[0023] [Figure 12] 10 shows another example of a tubular structure connecting the inlet port of the sample dispenser and the sample nozzle.

[0024] [Figure 13A-B] 1 illustrates an example of a hood that improves the effectiveness of a sample analysis system.

[0025] [Figure 14A-B] 13A and 13B show examples of sealing mechanisms for the hoods of FIGS. 13A and 13B.

[0026] [Figure 15A] 1 illustrates an example of the relative locations of a sample dispenser and a hood for a portion of a sample analysis system.

[0027] [Figure 15B] 1 shows a hood connected to a sample analysis system. DETAILED DESCRIPTION OF THE INVENTION

[0028] Biological tissue samples are used for microscopic and molecular analysis for clinical, diagnostic, and research applications. These samples are collected from patients for diagnostic analysis. and other medical or medical research settings. For example, cells / tissues are collected using biopsy brushes. is collected from the patient using a collection device such as a swab, or cutting tool, and placed in a sample container. Preparation of microscope slides for screening and / or diagnostic purposes. Once ready, the sample liquid is drawn through the filter by vacuum. After pressing the filter, the cells are transferred to a slide for observation and analysis. The sample liquid is drawn from the sample vial to the glass via a pipette or other suction-type device. Other non-liquid based applications for viewing cells under a microscope may be used. The apex method involves smearing cells or tissue directly onto the surface of a slide using a collection device. This includes:

[0029] In certain situations, it may be desirable to prepare multiple slides in a substantially similar manner. For example, by preparing two or more slides in the same way, the user can Alternatively, the test can be repeated to improve the reliability of the results. One of the slides can be used as a control slide. The rides can be processed at different times after undergoing the same or different treatments. In yet another example, one of the slides can be stained conventionally with histology, while the other slides In yet another example, one of the slides may be subjected to molecular staining. To quickly check the validity of the slides, they can be examined on-site, and other slides can be The sample can be processed in a laboratory for detailed cytological analysis. Preparing multiple slides using either of these methods typically reduces cross-contamination and provides reliable Use consumable parts to improve performance.

[0030] In various embodiments, a substantially equal amount of material is applied to each of a plurality of targets, such as slides. Systems, devices and methods for automated and simultaneous deposition of fluid samples A consumable part for depositing a fluid sample onto a target is described. a sample injection port (SIP) that allows for the sample to be deposited within the target area; and a hood that contains a filter and eliminates unintentional dispersion of the aerosolized sample.

[0031] Sample analysis system example

[0032] FIG. 1A shows an example of a sample analysis system 100. As shown therein, a sample The analysis system 100 includes a target holder that holds two targets 106 and 108 in place. 1A, for example, the target holder 110 includes a target Recesses that prevent the slots 106 and 108 from moving laterally once placed within the area. Targets include, for example, glass slides, cover slips, plastic The substrate may be a black substrate, a charged cytology slide, or a coated cytology slide. In some embodiments, the sample holder 110 may accommodate two or more different types of targets. The sample analysis system 100 includes a plurality of gas nozzles 102 and a These gas nozzles are connected to one or more sources of pressurized gas. When the nozzle is activated, gas is expelled from the nozzle toward the targets 106 and 108. Gases include, for example, compressed air, nitrogen, carbon dioxide, nitrous oxide, helium, and argon. etc.

[0033] Furthermore, the sample analysis system 100 includes a sample dispenser 120 (sample input port). The sample dispenser 120 includes a sample reservoir. 122 (also referred to as an inlet port or well) and a sample nozzle 134 and 1A includes at least two fluid paths 124 and 126 terminating at 136. As shown, sample reservoir 122 is connected to a sample reservoir 124 via fluid paths 124 and 126, respectively. and is fluidly coupled to both sample nozzles 134 and 136 .

[0034] Additionally, the sample analysis system 100 includes a sample dispenser holder 112 . The sample dispenser holder 112 has sample nozzles 134 and 136, each of which is a gas. to be positioned between the nozzles 102 and 104 and the targets 106 and 108. In particular, the sample nozzle is arranged to hold the sample dispenser 120. The nozzles 134 and 136 are the gases discharged by the gas nozzles 102 and 104, respectively. In some embodiments, the sample dispenser is positioned to be within the path of the sample dispenser. The sample dispenser 120 is attached to the sample analysis system 100 by the sample dispenser holder 112. In other embodiments, the sample dispenser 120 may be fixed to the sample analysis system. The sample dispenser holder 112 may be removable from the stem 100. After the sample dispenser 120 is inserted into the sample analysis system 100, the sample dispenser The sensor 120 is connected to the gas nozzles 102 / 104 and / or the target 106 / 108. The sample dispenser 120 may be passively and / or actively matched with a are consumable parts that are discarded after one (or a set number of) uses.

[0035] In operation, a user dispenses a fluid sample into sample reservoir 1 of sample dispenser 120. For example, a user may use a syringe to collect a fluid sample and deliver it to 22. The collected fluid sample may be discharged into the sample reservoir 122. The sample analysis system 100 detects the presence of the sample dispenser 120 (e.g., After the sample is delivered, the fluid sample may be automatically delivered to the sample reservoir 122. Now, the user must activate the fluid sample reservoir 122 so that the fluid sample is delivered to the sample reservoir 122. The sample may be delivered to another portion of the sample analysis system 100.

[0036] After the fluid sample is delivered to the sample reservoir 122, for example, by capillary action, The fluid sample is transported to sample nozzles 134 and 136. In this state, the sample dispenser 120 operates when the gas nozzles 102 and 104 are each activated. The fluid sample is configured to not flow through the fluid paths 124 and 126 unless will be done.

[0037] After the fluid sample is delivered to the sample reservoir 122, the gas nozzles 102 and 10 4 by the user (and / or automatically by the sample analysis system 100) The gas from the nozzle aerosolizes the fluid sample and propels it toward the target. 106 and 108. In some embodiments, the sample dispenser The nozzle 120 dispenses a predetermined amount of fluid when the gas nozzles 102 and 104 are each activated. A sample may be configured to be ejected from each sample nozzle 134 and 136. Advantageously, this allows the sample analysis system 100 to deliver a predetermined volume of the fluid sample to each target. In some embodiments, the sample dispenser 120, when the gas nozzle 102 / 104 is activated, substantially the same amount of fluid is pumped into the sump. A sample nozzle 134 / 136 may be configured to eject a sample. Thus, the sample analysis system 100 can consistently deliver the same predetermined volume of fluid sample to each target. In some embodiments, the flow exiting each sample nozzle can be The volume of the sample is determined, at least in part, by the duration of operation of the gas nozzle and / or the nozzle size. The pressure can be based on the gas pressure at the

[0038] In some embodiments, after the fluid sample is delivered to the sample reservoir 122, The sample analysis system 100 may include some fluid (or A fluid (e.g., a distilled water solution) may be delivered to the sample reservoir 122. At least one of water, saline, different concentrations of ethanol, buffer solution, isotonic solution, etc. may include a combination of:

[0039] In FIG. 1A, the sample analysis system 100 includes a target holding device. a sample dispenser 120 having two sample nozzles and two gas In some embodiments, the sample analysis system 100 includes an additional target nozzle. may include a target holder and / or a target holder that holds three or more targets. In these embodiments, the sample dispenser 120 may be configured such that the number of sample nozzles is proportional to the system Additional samples may be added to match the number of samples that can be held by the system 100. A pull nozzle (and a corresponding number of additional gas nozzles) may be included.

[0040] In some embodiments, each target is dispensed from two or more sample nozzles. In these embodiments, each target has a surface that is capable of receiving a sample. It is possible to have more than one sample patch dispensed.

[0041] FIG. 1B shows a sample dispenser holder 112 integrated into a sample analysis system. sample nozzles (e.g., air nozzle 104 and sample nozzle 136, respectively) Another feature of the sample analysis system 100 is that it ensures proper alignment and effectiveness of the air nozzles that are Here is an example:

[0042] Example of a Expendable Sample Injection Port (SIP)

[0043] 2 to 12 show sample injection ports (SIPs) or sample dispensers (e.g., 1A and 1B show various embodiments and features of the sample dispenser 120. Although shown and described as different embodiments, the features described in any embodiment may be It is not limited to that particular embodiment and may be combined with a sample dispenser described in another embodiment. It can be combined.

[0044] FIG. 2 illustrates the sample dispenser 120 and gas nozzles 102 and 104 of FIG. 1A. As shown in FIG. 2, the sample dispenser 220 includes a first sample nozzle. a first tubular structure 224 (e.g., fluid pathway 124 in FIG. 1A) terminating in a tube 234; A second tubular structure 226 (e.g., fluid path 12 in FIG. 1A) terminates in a sample nozzle 236. 6), and sample reservoir 222 (e.g., inlet port 122 in FIGS. 1A and 1B). The sample dispenser 220 includes a tubular structure 224 connected to the sample reservoir 222. It also includes a first restrictor (also of tubular structure) 225 that is fluidly coupled to the san. The pull dispenser 220 includes a first fluid coupling tubular structure 226 to the sample reservoir 222. 2 restrictors 227 (also of tubular construction).

[0045] In some embodiments, the cross-sectional area of ​​the tubular structure 224 is greater than the cross-sectional area of ​​the restrictor 225. The cross-sectional area of ​​the tubular structure 226 is larger than the cross-sectional area of ​​the restrictor 227. In some embodiments, the tubular structures 224 / 226 and the restrictors 226 / 227 are: It may have at least one of the following cross-sectional shapes: circular, oval, rectangular, and polygonal. In some embodiments, the sample reservoir 222 has a volume of 5 μL (microliters). In some embodiments, the sample reservoir 222 may have a volume of about 150 μL. It may have a volume of less than 2 milliliters.

[0046] As shown in FIG. 2, tubular structure 224, tubular structure 226, restrictor 225, and and restrictor 227 have the same cross-sectional shape and area throughout their length. However, in some embodiments, the tubular structure 224, the tubular structure At least one of the structure 226, the restrictor 225, and the restrictor 227 is A cross section that varies over its length (e.g., as described in the example shown in Figure 12) In these embodiments, the average cross-sectional area of ​​the tubular structure 224 is less than that of the restrictor 225. the average cross-sectional area of ​​the tubular structure 226 is greater than the average cross-sectional area of ​​the restrictor 227. is larger than the cross-sectional area.

[0047] In some embodiments, restrictors 225 and 227 are each between about 0.1 mm and It may be about 2 mm long, for example, with increments of 0.1 mm. The distance between the tubular structures 224 and 226 can be about 1 mm to about 2 mm, for example, 0. In some embodiments, restrictors 225 and 227 may have increments of 0.1 mm. The cross section has a diameter of about 0.5 mm to about 1.5 mm, for example, in increments of 0.1 mm. In some embodiments, the cross section of the restrictors 225 and 227 may be a circle. , may be a circle having a diameter of less than 0.8 mm. In some embodiments, the tubular structure 22 4 and 226 may each be about 10 mm long. In some embodiments, the tubular structure The cross section of structures 224 and 226 may be a circle with a diameter of 0.8 mm.

[0048] As shown in FIG. 2, tubular structures 224 and 226 each include a sample dispenser. The sample nozzles 234 and 236 project from the sensor 220. The protrusions allow the gas nozzles to be in close proximity to the exterior openings of the tubular structures 224 and 226. Additionally, the protrusions allow the air to flow smoothly rather than being disturbed by other surfaces. A gentle airflow can be applied directly to the sample in the airflow. The location and stability of the air nozzles affect the spray pattern produced, and the air Position the nozzle so that it can flow directly through the end of the tube without disturbance In some embodiments, the length of the protrusions may be about 1 mm. In some embodiments, the length of the sample nozzle may be between 0.1 mm and 1 mm, for example, 0. In some embodiments, the length of the sample nozzle may be in increments of 0.1 mm. It may be ∼10mm, for example with 1mm increments.

[0049] In some embodiments, the tubular structure 224, the tubular structure 226, the restrictor 225, and and / or the inner surface of the restrictor 227 may be made of a hydrophobic material, a hydrophilic material, and / or a pre-existing It may be coated with (and / or made of) a material with known hydrophilic / hydrophobic properties. For example, the material may be Teflon or similar to limit the resistance of the fluid flowing through the inner tube. It can be something.

[0050] As shown in FIG. 2, the sample reservoir 222 is a conical (or pyramidal) and connected to restrictors 225 and 227 at the narrow conical ends of the reservoir. In other embodiments, the sample reservoir 222 has a prismatic or cylindrical shape. In these embodiments, the sample reservoir 222 may have a prismatic or cylindrical shape. The sample reservoir 222 may be connected to the restrictors 225 and 227 through holes on the sample reservoir 222. do.

[0051] In operation, a user may deliver a collected fluid sample to the sample reservoir 222. For example, a user may use a syringe to collect a fluid sample and then The fluid sample can be discharged into the sample reservoir 222. After delivery to the reservoir 222, capillary action occurs, drawing the fluid sample into the restrictor 225 and and 227, while the fluid sample flows within the tubular structures 224 and 226. It's not crowded.

[0052] The relatively small cross-sectional area of ​​the restrictors 225 and 227 allows for the transport of fluid through them. Therefore, restrictors 225 and 227 increase the amount of force required to At least a portion of the force acting on the fluid sample due to gravity can be counteracted. Without the restrictors 225 and 227, gravity would force the fluid sample through the tubular structure 224 and and 226, and the sample is delivered to the sample reservoir 222. However, the restrictor 225 and When restrictors 225 and 227 are present, the fluid sample is transported to the edges of restrictors 225 and 227. but do not enter the tubular structures 224 and 225, which are sample nozzles 234 and 235, respectively. No gas flows out of 236 (unless gas nozzles 202 and 204 are activated, respectively).

[0053] In some embodiments, the fluid sample is delivered to the sample reservoir 222 and before the sample is transported by capillary action to the edges of the restrictors 225 and 227. During or after, the gas nozzles 202 and 204 may be turned on or off by the user (and / or The nozzle can be operated automatically by the sample analysis system. The sample first enters the tubular structures 224 and 226, then the sample nozzle 234 and 236 into the gas flow, depleting the fluid sample within the tubular structures 224 and 226. In particular, the gas from the nozzle is aerosolized toward the target. Negative pressure is applied to the tubes 234 and 236 to force the fluid sample out of the tubular structures 224 and 226. It can be discharged.

[0054] The ejected fluid sample is then directed by a gas nozzle onto a target surface (e.g., In some embodiments, the gas nozzle is aerosolized onto the slide. ~0.5 seconds, e.g., 0.1 second increments, with a maximum positive pressure of 200 kPa per nozzle. In some embodiments, the gas nozzle may be operated at a pressure in the range of 10 kPa to 190 kPa. In some embodiments, the gas nozzle may be operated at a pressure of 1 mm or less. In some embodiments, the gas nozzle may have a diameter of 0.2 mm to 2.0 mm, e.g. For example, the aperture diameter may be in increments of 0.1 mm.

[0055] Advantageously, the amount of fluid sample deposited on the surface of the target is controlled by the sample nozzle 234. and 236 based on the duration and pressure of the gas applied.

[0056] Furthermore, the tubular structures 224 and 226 and / or the restrictors 225 and 226 may The relative volumes of the 7 can affect the relative rates of sample deposition. If you require substantially the same amount of sample deposition, restrictor 225 / 227 and tube The shaped structures 224 / 226 can be designed to be symmetrical about the inlet port 222. Thus, in some embodiments, the volume of sample deposited on the target is This may depend on the pressure, the duration of nozzle actuation, and the dimensions of the tubular structure.

[0057] In the example shown in FIG. 2, tubular structures 224 and 226, as well as restrictors 225 and and 227 are shown as being straight. In some embodiments, the tubular structures 224, tubular structure 226, restrictor 225, and / or a small number of restrictors 227. At least a portion of the sample is directed towards and / or to sample nozzles 234 and 236, respectively. may be curved apart.

[0058] 3 shows another example of a sample dispenser. Sample dispenser 320 is shown in FIG. The first and second tubular structures 324 and 326 may be at an obtuse or acute angle (theta) relative to one another. 2 except that the sample dispenser 220 is similar to the sample dispenser 220 of FIG. In some configurations, the spray pattern from the nozzle may be preferable for some applications. In some embodiments, the angle theta in FIG. 3 can range from 180° to 30°. In some embodiments, the angle theta in FIG. 3 is the angle between the first and second tubular structures 324 and 326. 26 are both oriented in substantially the same direction (i.e., towards the target) and / or parallel to Additionally or alternatively, the first and second tubular The structures 324 and 326 may be angled relative to a plane normal to the target.

[0059] FIG. 4 shows yet another example of a sample dispenser. Sample dispenser 420 includes: , for interfacing with air nozzles 402 and 404 of a sample analysis system The sample dispenser 420 includes air nozzle interfaces 422 and 424. 1A, except for the air nozzle interface. The faces 422 and 424 are positioned and aligned relative to the tubular structures 424 and 426, respectively. For example, air nozzle interfaces 422 and 424 are fixed in position. and tubular structures 424 and 426 may be part of the same rigid structure (not shown in FIG. 4). (not yet).

[0060] Advantageously, air bubbles are fixed in position and angle relative to the tubular structures 424 and 426. Nozzle interfaces 422 and 424 are connected to air nozzles 402 and 404, respectively. This can reduce deposition variations resulting from mismatch between the nozzle 4 and the tubular structures 424 and 426. For example, air nozzle interfaces 422 and 424 are connected to air nozzles 402 and 404. The gas discharged by the sample nozzle 434 and the The air nozzles 402 and 404 and the tubular structure 424 and 436 are guided to intersect. The misalignment between the sample dispenser holder (e.g., FIG. 1A and and sample dispenser holder 112 of FIG. 1B) and / or sample dispenser ( For example, this can result from improper manufacturing of 120, 220, 320, or 420. Thus, misalignment can result in an incorrect location of the nozzle relative to the sample dispenser holder and and / or may also result from positioning.

[0061] In some embodiments, as shown in FIG. 4, an air nozzle interface 422 and / or 424 faces the air nozzle rather than the sample nozzles 434 and 436. Wider openings at the ends (e.g., by having conical or pyramidal openings) Advantageously, the wider opening is at the air nozzle interface 422. / 424 is a larger nozzle between the air nozzles 402 / 404 and the sample dispenser 420. This may allow for compensation for any mismatch.

[0062] 5A and 5B show yet another example of a sample dispenser. The sample dispenser 520 shown in FIG. 5B is similar to the sample dispenser 120 of FIG. 1B. Inlet port 522 is connected to the sump via fluid paths 524 and 526, respectively. The sample is fluidly coupled to nozzles 534 and 536. As shown in FIG. The dispenser 520 is opposite the inlet port and sample nozzle of the sample dispenser. The opposite end further includes a recess that allows the user to insert the sample dispenser into the sample holder. 5A and 5B) to properly place the sample in the pull analysis system. You can hold the dispenser firmly.

[0063] In some embodiments, the depression allows the SIP to be grasped normally with one hand, e.g., during needle aspiration procedures. The user's or technician's finger is used to deposit sample material into the inlet port 522 using a needle. It includes a protective cover (not shown in Figures 5A and 5B).

[0064] In some embodiments, the sample nozzle 534 of the sample dispenser 520 and 536, the aerosolized sample exits in the opposite direction and then reaches the target (e.g., 5A and 5B). Port 522 is configured to be a narrow opening, as shown in FIGS. 5A and 5B. The sample nozzles 534 and 536 deposit the sample in a circular pattern onto the target in a single layer. That is, the sample nozzle may be configured to deposit sample onto the target. When stacked, overlapping sample cells are minimized and preferably eliminated. It can be configured to ensure that

[0065] In some embodiments, the inlet port 522 passively adds buffer to the sample material. The casing may include a notch (not explicitly shown in FIGS. 5A and 5B) that allows for Adding a buffer solution advantageously distributes the sample material evenly over the target. In one example, the buffer solution is phosphate buffered saline (PBS), which Disodium hydrogen phosphate, sodium chloride, and in some formulations, potassium chloride and and potassium dihydrogen phosphate.

[0066] In some embodiments, the buffer may be selected as one or more of the following: TAPS ([tris(hydroxymethyl)methylamino]propanesulfonic acid), bicine (2-(bis(2-hydroxyethyl)amino)acetic acid), Tris(tris(hydroxymethyl) (2-amino-2-(hydroxymethyl)propane-1,3 -diol), Tricine (N-[tris(hydroxymethyl)methyl]glycine), TA PSO(3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropane HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), sulfonic acid), TES (2-[[1,3-dihydroxy-2-(hydroxymethyl)propanol [N-2-yl]amino]ethanesulfonic acid), MOPS (3-(N-morpholino)propanol PIPES (piperazine-N,N'-bis(2-ethanesulfonic acid)) , cacodylic acid (dimethylarsenic acid), and MES (2-(N-morpholino)ethanesulfonyl ester) phosphate).

[0067] In some embodiments, the buffer and sample material are delivered to the inlet port 522. In one example, a microfluidic device is used to actively combine the sample material and the buffer. A scale fan may be installed adjacent to the inlet port. In yet another example, flow pulsation may be used to perform active mixing. In yet another example, pressure may be increased to perform the mixing operation. A buffer solution can be added to the inlet port.

[0068] 6A and 6B show yet another embodiment of a sample dispenser. The dispenser 620 is similar to the sample injection port (SIP) shown in FIG. The pull nozzles 634 and 636 are configured to be parallel to each other. Both SIPs shown in Figures 5B and 6A / 6B have a sacrificial gas flow when the gas nozzle is activated. Discharge sample material in the forward direction (relative to the placement of the SIP in the sample analysis system) However, as shown in FIGS. 6A and 6B, This configuration of the nozzle resulted in a circular deposition field on the target produced by SIP, shown in Figure 5. elliptical deposition footprint on the target compared to the This results in a

[0069] In the embodiment shown in FIGS. 6A and 6B, the inlet port 622 is located within the SIP 620. Fluidically coupled to sample nozzles 634 and 636 by embedded fluid paths In one example, the embedded fluid pathway may be linear. The fluid path includes the fluid path and the inlet port 622 and the sample nozzles 634 and 636. and may be curved to minimize the distance traversed by the sample material between them.

[0070] 7A and 7B show yet another embodiment of SIP. Sample input port 720 is When the gas nozzles are activated, the sample nozzles 734 and 736 pump the sample material back configured to discharge in a direction (relative to the placement of the SIP in the sample analysis system) 6A. The sample dispenser 620 shown in FIG. 6A is similar to the sample dispenser 620 shown in FIG. and similar to the embodiment shown in FIG. 6B, parallel sample nozzles allow the gas nozzles When activated, this results in an elliptical deposition footprint on the target.

[0071] Embodiments of the disclosed technology include multiple sample nozzles (e.g., the sample nozzles in FIGS. 5-7). nozzles 534 / 536, 634 / 636, and 734 / 736) simultaneously, This advantageously allows for consistent deposition on multiple targets. SIP (opposite deposition of a circular footprint on the target) shown in Figure 5A and Figure 5B. Using a sample nozzle facing in the opposite direction will allow 50%-70% of the cells to slide. The deposition will be on a field, but will deposit an oval footprint on the target. 6A / 6B or 7A / 7B including parallel nozzles arranged in a and increase the amount of cells deposited on the target, increasing it to 80%-95%. This reduces cell loss.

[0072] In some embodiments, the sample dispenser is configured to: The sample material may be configured to deposit unequal amounts of sample material onto one target. In this case, this is achieved by using unequal gas pressures in the two gas nozzles. In another example, this may be achieved by disposing the first gas nozzle or the first sample nozzle in a Each has a different size or shape compared to the second gas nozzle or the second sample nozzle. In yet another example, this may be achieved by increasing the first fluid path compared to the second fluid path. In yet another example, a different material coating may be used for the first fluid path. The diameter or length of the fluid passage may be different from that of the second fluid passage. In the example, a blocker material is used to target one target compared to the other. It may be possible to deposit larger amounts of sample material.

[0073] In some embodiments, the sample material is used for fine needle aspiration (FNA) from the patient. Freshly collected and separated into targets (e.g., slides) for examination and / or investigation. Use a hollow needle containing cells ready to be delivered to the inlet port (e.g., Figure 5, respectively). 7 (522, 622, or 722 in FIG. 7). The pull material may be premixed with a buffer solution, and the mixed fluid sample may be applied to the target. It may be added to an inlet port for dispensing.

[0074] In some embodiments, the sample dispenser (or the device in contact with the sample material) The sample dispenser (part of the sample dispenser) may be molded using a material with low surface energy. If the surface energy of the material is high, the liquid will spread over the entire surface of the material. The use of a material with a low viscosity ensures that the liquid is collected. The sample material (or a mixture of sample material and buffer, if appropriate) is Ensure that the sample is expelled through the sample nozzle instead of remaining in the inlet port. So, the materials used in making it are listed in the table below (which is in millinewtons per meter (m The surface energy may also be selected from the group consisting of: [Table 1]

[0075] In some embodiments, the SIP shown in FIGS. 5-7 may further include an air nozzle. In other words, the SIP consumable includes both the sample nozzle and the air nozzle. These can be optimally aligned during manufacturing and the air nozzles (of the sample analysis system) The gas discharged from the sample nozzle (which may be connected to a pressure source that is part of the sample nozzle) is passed through the sample nozzle. It may be ensured that all sample material is expelled from the pathway.

[0076] FIG. 8 shows a sample dispenser shown in FIGS. 6A and 6B. As shown in FIG. 8, the sampler dispenser 820 includes an inlet port 822 includes a curved fluid path 824 embedded within the structure of the sample dispenser and 826, which are fluidly coupled to sample nozzles 834 and 836, respectively. As mentioned above, the parallel configuration of the sample nozzles results in an elliptical deposition flux on the target. The resulting prints are shown in slides 806 and 808 of FIG. In the synthesis, up to 70-80% of the cells are deposited on the target, resulting in lower levels cell loss is achieved.

[0077] FIG. 9A shows an example of an inlet port of a sample dispenser, and FIG. 9B shows an example of the inlet port of FIG. 9A. FIG. 10A shows another example of an inlet port for a sample dispenser. 10B shows the geometry of the inlet port of FIG. 10A. 9B is shallow and wide (large angle between the walls of the inlet port). While the inlet port 1022 in FIG. 10B is configured to be deep and narrow (the walls of the inlet port The geometry of the inlet port is designed to minimize the angle between the inlet and outlet ports. Maximize the draw of the sample (or sample material) and then The nozzle may be configured to eject the sample when activated.

[0078] In some embodiments, the inlet port is configured to hold between 5 μL and 150 μL. In one example, 2-10 μL of sample and 20 μL of buffer solution can be introduced into the inlet port. In some embodiments, the inlet port allows the sample to be deposited into a volume. All overflows will be detected as long as the volume (or sample and buffer volume) does not exceed this level. In some embodiments, the inlet may include a "max fill line" to prevent overflow. The port simplifies the use of an FNA needle to deposit the sample into the sample dispenser The needle guide may include a needle guide that

[0079] Figure 11 shows the tubular structure ( As mentioned above in the context of FIG. 2, the restrictor 1125 and 1127 are of smaller cross section than fluid paths 1124 and 1126; This allows sample material placed in the inlet port 1122 to enter the list via capillary action. The fluid flows to the edge of the reactor until the gas nozzles 1102 and 1104 are activated. Allows you to stay out of the way.

[0080] As shown in FIG. 11, the fluid paths 1124 and 1126 are connected to the restrictor (112 5 and 1127) from their starting points at their respective sample nozzles 1134 and 1136 are uniformly cylindrical. That is, the cross-sectional area of ​​the fluid paths is proportional to their length. does not change over time.

[0081] Figure 12 shows the tubular structure ( 12 shows another example of a cross section of a fluid path 1224 and and 1226 are not uniformly cylindrical and reach the sample nozzles 1234 and 1236. The cross-sectional area of ​​the fluid path is therefore , the average cross-sectional area of ​​fluid paths 1224 and 1226 varies along their lengths, The cross-sectional area of ​​the inducers 1225 and 1227 is larger than that of the gas nozzles, which actuate the sump. When negative pressure is generated at the nozzle, only the fluid sample enters the fluid path and is expelled from it. The varying cross-sectional area of ​​the fluid path shown in FIG. 12 advantageously allows for complex This allows for reduced tooling of the device.

[0082] 2 to 12 are consumable parts of the sample analysis system, and are used for each use (or predetermined use). Various implementations of SIP or sample dispensers configured to be discarded after a certain number of times The SIP features described herein are characterized by the formation of a single layer (overlapping) of sample material. Ensure that the number of cells (minimal number of cells) is uniformly and consistently distributed across multiple targets. These features include the geometry of the inlet port (e.g., size and depth), fluid path design, sample nozzle tip geometry, and overall SIP geometry. These include, but are not limited to:

[0083] Examples of consumable foods

[0084] Another consumable part of the sample analysis system is the hood shown in Figures 13-14. Provides a seal with the container (e.g., slide) and prevents unintended contamination of the aerosolized sample. Prevents unwanted dispersion and cross-contamination, thereby improving the reliability and effectiveness of sample analysis. do.

[0085] 13A and 13B show examples of hoods that improve the effectiveness of sample analysis systems. As shown therein, the hood comprises a body 1 having an upper portion, a middle portion, and a lower portion. 305. The top portion includes a holder 1310 and an aerosol opening 1315. The middle portion includes a dye opening 1320 .

[0086] In some embodiments, the sample nozzle of the SIP is located above the aerosol opening 1315. and when the gas nozzle of the sample analysis system is activated, the aerosolized gas is Ensure that the sample is deposited on the target and the hood contains the sample. and prevent unintentional dispersion or cross-contamination of the workspace. , one or more dyes used in sample analysis are targeted through stain opening 1320 It can be added to the sample deposited on top.

[0087] In some embodiments, as shown in FIGS. 13A and 13B, an aerosol opening The portion 1315 and the dye opening 1320 are rectangular in shape. Either or both of the sol and dye openings may be circular, elliptical, or polygonal.

[0088] The hood body 1305 allows the hood to be properly attached to a sample analysis system. The lower portion of the hood includes a seal 1330, which is an aerosolized sample material attached to a target and dispensed onto the target. The hood also has a second notch ( 13A and 13B), which (together with the notch 1325) The notch helps ensure the card is properly positioned in the sample analysis system. The hood (and the bottom portion of the hood 1305) are sealed during the spraying and dyeing process. This allows the target to be properly positioned.

[0089] 14A and 14B show examples of sealing mechanisms for the hoods of FIGS. 13A and 13B. As discussed above, the seal 1430 of the hood 1405 prevents the deposited sample from As shown in Figures 14A and 14B, the seal 1430 When the hood comes into contact with the target (e.g., a microscope slide), it presses down to form a seal. can ensure that the seal is more reliable and effective than a simple rubber gasket. The clamp 1432 includes a clamp 1432.

[0090] In some embodiments, the hood is made of one of the following materials (or a combination thereof): It can be made from one of: acrylic (poly(methyl methacrylate)), cyclic olefins olefin copolymer, polystyrene, styrene acrylonitrile, polycarbonate, or polypropylene.

[0091] Figure 15A shows the relationship between the sample dispenser and the hood for part of a sample analysis system. As shown above, the SIP1520 is designed so that the sample nozzle is positioned in the aerodynamic direction of the hood. The sample analyzer is positioned so that it is directly above the sol opening, thereby allowing the gas Depositing the aerosolized sample onto the target when the nozzle is activated. As shown in Figure 15A, the dye opening can be opened using the notch below the dye opening. and is connected to a part (or tab) of the sample analysis system.

[0092] FIG. 15B is a detailed version of an exemplary connection between the hood and the tub of a sample analysis system. The tabs indicate two circular openings into which one or more needles containing dye or coloring agents can be inserted. The aerosolized sprayed sample contains droplets (1541 and 1542) and is sprayed onto the target. This allows for the deposition of dyes or colorants onto the sample.

[0093] In some embodiments, based on the description provided herein, the following technical solutions can be implemented.

[0094] A1. To distribute substantially the same volume of liquid-based sample material to multiple targets a sample dispenser for dispensing a sample from a sample container, the sample dispenser comprising: a first chamber having a first interior volume and a first inner perimeter; a tubular structure, (i) a first opening of the first tubular structure, the first opening being connected to a first gas nozzle and a first A first gas nozzle is disposed between the first target and the second target, and the first gas nozzle discharges gas toward the first target. (ii) the first periphery is configured to exit through a second opening in the first tubular structure. Inducing capillary action to fill the first tubular structure with a liquid-based sample material. a first tubular structure selected to be able to receive the fluid from the first tubular structure, and a second internal volume and a second inner circumference; a second tubular structure, wherein (i) a first opening of the second tubular structure is connected to a second gas nozzle; A second gas nozzle is disposed between the first target and the second target, and a gas is directed toward the second target. (ii) the second periphery is configured to vent the gas through a second opening in the second tubular structure; and inducing capillary action to fill the second tubular structure with a liquid-based sample material. and a second tubular structure selected to be able to: the second opening of the first tubular structure is connected to the second opening of the second tubular structure; A sample dispenser is located adjacent to the opening.

[0095] A2. The second openings of the first and second tubular structures are configured to receive sample material. A dispenser of solution A1 mechanically coupled to a reservoir of the same.

[0096] A3. The second openings of the first and second tubular structures are oriented in substantially the same direction. Dispenser of solution A1.

[0097] A4. The angle between the first openings of the first and second tubular structures is between 90 degrees and 180 degrees. Dispenser of solution A1.

[0098] A5. The first gas nozzle and the first opening of the first tubular structure are When the gas is evacuated, the sample material within the first tubular structure is substantially completely emptied and the first A dispenser of solution A1 positioned to face the target.

[0099] A6. The second gas nozzle and the first opening of the second tubular structure are When the gas is evacuated, the sample material within the second tubular structure is substantially completely emptied and the second A dispenser of solution A1 positioned to face the target.

[0100] A7. The cross-sectional shape of the first tubular structure is one of a circle, an ellipse, and a polygon. , dispenser of solution A1.

[0101] A8. The dispenser of solution A1, wherein the first and second tubular structures are parallel to each other.

[0102] A9. A system for sample analysis, which outputs gas to a first target. a first gas nozzle configured to eject gas toward a second target; a second gas nozzle configured to hold the first and second targets; A target holder with a liquid-based sump of substantially the same volume for multiple targets. a sample dispenser for dispensing sample material, the dispenser having a first internal volume and a first tubular structure having a first inner periphery and a first opening in the first tubular structure; (ii) a first periphery, the first periphery being disposed between the first gas nozzle and the first target; Filling the first tubular structure with a liquid-based sample material through a second opening in the first tubular structure. a first tubular structure selected to be capable of inducing capillary action to fill the first tubular structure; and a second tubular structure selected to be capable of inducing capillary action to fill the first tubular structure. and a second inner perimeter, wherein (i) the second tubular structure the first opening is disposed between the second gas nozzle and the second target; and (ii) The second tubular structure is surrounded by a second opening and filled with a liquid-based sample material. a second tubular structure, selected to be capable of inducing capillary action to fill the tubular structure; the first internal volume and the second internal volume are substantially the same, and the first tubular structure the second opening of the first tubular structure is proximate to the second opening of the second tubular structure.

[0103] A10. Sample dispenser arranged to hold sample dispensers over multiple targets The system of Solution A9 further comprising a pull dispenser holder.

[0104] A11. A system of solution A9 in which the sample dispenser is replaceable.

[0105] A12. The second openings of the first and second tubular structures are for receiving sample material. A system of solution A9 mechanically coupled to a common reservoir.

[0106] A13. The second openings of the first and second tubular structures are oriented in substantially the same direction. The system has solution A9.

[0107] A14. The acute angle between the first openings of the first and second tubular structures is between 90 degrees and 180 degrees. There is a system with solution A9.

[0108] A15. The first gas nozzle and the first opening of the first tubular structure are When the gas is exhausted, the sample material in the first tubular structure is substantially completely emptied and the first The solution A9 system is positioned to face the target.

[0109] A16. The second gas nozzle and the first opening of the second tubular structure are When the second tubular structure is exhausted, the sample material in the second tubular structure is substantially completely emptied. The solution A9 system is positioned to face the target.

[0110] A17. The cross-sectional shape of the first tubular structure is one of a circle, an ellipse, or a polygon. Solution A9 system.

[0111] A18. The system of solution A9, wherein the first and second tubular structures are parallel to each other.

[0112] In some embodiments, based on the description provided herein, the following technical solutions can be implemented.

[0113] B1. A device for dispensing fluid samples, including an inlet for introducing sample material. a first sample port fluidly coupled to the inlet port for discharging sample material; a first sample nozzle and a second sample nozzle, and a tubular junction connecting the inlet port to the first sample nozzle. a first tubular fluid path terminating in a sample nozzle and a second tubular fluid path terminating in a second sample nozzle. and a tubular junction having a cross-sectional area that is equal to or larger than the cross-sectional area of ​​the first tubular fluid path and the cross-sectional area of ​​the second tubular fluid path. The cross-sectional area of ​​the tubular junction is smaller than the average cross-sectional area of ​​the tubular fluid path, allowing fluid to enter via capillary action. A device selected to draw sample material from the inlet port into the tubular junction.

[0114] B2. A first sample nozzle is disposed between the first gas nozzle and the first target. and a first gas nozzle is activated to discharge gas toward the first sample nozzle. and a first sample nozzle ejecting sample material toward a first target. Measure B1 device.

[0115] B3. The depth and shape of the inlet port increases the drawing of sample material into the tubular joint. The device of solution B1 is selected to cause

[0116] B4. The inlet port includes a notch that allows for the addition of a buffer solution to the sample material , Solution B1 device.

[0117] B5. A solution in which the buffer is actively mixed with the sample material using an active mixing means. Device for resolution B4.

[0118] B6. Active mixing means include flow pulsation, acoustic mixing, ultrasonic mixing, or mechanical mixing. The apparatus of solution B4, including at least one microscale fan.

[0119] B7. The device of any of Solutions B4-B6, wherein the buffer is phosphate buffered saline.

[0120] B8. The first and second sample nozzles are oriented in the same direction. The device of solution B1.

[0121] B9. At least a portion of the first tubular fluid path is connected to at least a portion of the second tubular fluid path. The device of solution B8 is parallel to.

[0122] B10. The cross-sectional area of ​​the first tubular fluid path is uniform throughout its length. Solution B1 device.

[0123] B11. The cross-sectional area of ​​the first tubular fluid path is from the tubular junction to the midpoint of the first tubular path. Increase and decrease from the midpoint to the first sample nozzle, device of solution B1.

[0124] B12. At the opposite end from the first and second sample nozzles, insert the inlet port. The device of Solution B1, further comprising a grip portion adjacent to the port.

[0125] B13. The device of solution B12, wherein the grip portion comprises an indentation.

[0126] B14. Improve sample material deposition on targets in sample analysis systems a hood for a vehicle, comprising a top panel, a front panel, a left panel, a right panel, and a rear panel; a body including a sample holder, the top panel having a first opening configured to receive sample material; a top panel having a mouth, the top panel being parallel to the top panel and perpendicular to the back panel, the top panel being positioned adjacent to the back panel; a lower panel showing a dyeing process following deposition of sample material on the target; a second opening for receiving a dye or colorant, and supporting the body and the lower panel; a bottom rim, the bottom rim configured to adhere to the target prior to deposition; Do.

[0127] B15. The first opening is raised above the top panel, which then covers the front panel. Extends beyond, hood of solution B14.

[0128] B16. The rear panel is designed to securely attach the hood to the sample analysis system. Hood of solution B14 with formed notches.

[0129] B17. The bottom rim is configured to improve the seal between the bottom rim and the target. Hood with lamp, solution B14.

[0130] The above parts are intended to illustrate some types of possibilities. It is merely an embodiment and does not limit the scope of the present technology in any way.

[0131] In view of the foregoing, specific embodiments of the present invention are described herein for purposes of illustration. However, it will be understood that various modifications may be made without departing from the scope of the invention. . The present application provides the following aspects of the invention. (Aspect 1) A system for distributing substantially equal volumes of liquid-based sample material to multiple targets. A simple dispenser, a first tubular structure having a first interior volume and a first inner circumference; (i) a first opening of the first tubular structure is connected to a first gas nozzle and a first target; and the first gas nozzle is disposed between the first target and the second target, and the first gas nozzle discharges gas toward the first target. configured to (ii) the first periphery is connected to the liquid vessel through a second opening of the first tubular structure; Capillary action can be induced to fill the first tubular structure with sample material from the source. a first tubular structure selected to be a second tubular structure having a second interior volume and a second inner circumference; (i) a first opening of the second tubular structure is connected to a second gas nozzle and a second target; and the second gas nozzle is disposed between the first target and the second target, and the second gas nozzle discharges gas toward the second target. configured to (ii) the second periphery is connected to the liquid vessel through a second opening of the second tubular structure; Capillary action can be induced to fill the second tubular structure with sample material from the source. a second tubular structure selected to be The first internal volume and the second internal volume are substantially the same, and the first tubular structure the second opening of the first tubular structure is adjacent to the second opening of the second tubular structure. Pensa. (Aspect 2) the second openings of the first and second tubular structures being adapted to receive sample material; 10. The dispensers of embodiment 1, wherein the dispensers are mechanically coupled to a common reservoir. (Aspect 3) the second openings of the first and second tubular structures are oriented in substantially the same direction; 2. The dispenser of embodiment 1, (Aspect 4) The angle between the first openings of the first and second tubular structures is between 90 degrees and 180 degrees. 2. The dispenser of claim 1. (Aspect 5) The first gas nozzle and the first opening of the first tubular structure When the nozzle discharges the gas, substantially all of the sample material within the first tubular structure is evacuated. 2. The dispenser of claim 1, wherein the dispenser is positioned so as to be kicked toward the first target. (Aspect 6) The second gas nozzle and the first opening of the second tubular structure When the nozzle discharges the gas, substantially all of the sample material within the second tubular structure is evacuated. 2. The dispenser of claim 1, wherein the dispenser is positioned so that the ejection beam is deflected toward the second target. (Aspect 7) The cross-sectional shape of the first tubular structure is one of a circle, an ellipse, and a polygon; 2. The dispenser of embodiment 1. (Aspect 8) 2. The dispenser of embodiment 1, wherein the first and second tubular structures are parallel to one another. (Aspect 9) 1. A system for analyzing a sample, comprising: a first gas nozzle configured to discharge gas toward a first target; a second gas nozzle configured to discharge gas toward a second target; a target holder arranged to hold the first and second targets; Dispense substantially equal volumes of liquid-based sample material onto multiple targets a dispenser, the dispenser comprising: a first tubular structure having a first interior volume and a first inner circumference; (i) a first opening of the first tubular structure is connected to the first gas nozzle and the first It is placed between the target and (ii) the first periphery is exposed to the liquid through a second opening of the first tubular structure; inducing capillary action to fill the first tubular structure with a base sample material. a first tubular structure selected to be able to a second tubular structure having a second interior volume and a second inner circumference; (i) a first opening of the second tubular structure is connected to the second gas nozzle and the second It is placed between the target and (ii) the second periphery is exposed to the liquid through a second opening of the second tubular structure; inducing capillary action to fill said second tubular structure with a base sample material. a second tubular structure selected to be able to The first internal volume and the second internal volume are substantially the same, and the first tubular the second opening of the first tubular structure is adjacent to the second opening of the second tubular structure. Stem. (Aspect 10) a sample dispenser positioned to hold the sample dispenser over the plurality of targets; 10. The system of embodiment 9, further comprising a pull dispenser holder. (Aspect 11) 10. The system of embodiment 9, wherein the sample dispenser is replaceable. (Aspect 12) The second openings of the first and second tubular structures are adapted to receive the sample material. 10. The system of embodiment 9, wherein the plurality of sensors are mechanically coupled to a common reservoir for detecting the presence of a substance. (Aspect 13) the second openings of the first and second tubular structures are oriented in substantially the same direction; 10. The system of claim 9, wherein (Aspect 14) The acute angle between the first openings of the first and second tubular structures is between 90 degrees and 180 degrees. A system according to embodiment 9. (Aspect 15) The first gas nozzle and the first opening of the first tubular structure When the nozzle discharges gas, substantially all of the sample material within the first tubular structure 10. The system of embodiment 9, wherein the system is positioned to be open and directed toward the first target. (Aspect 16) The second gas nozzle and the first opening of the second tubular structure When the nozzle discharges gas, substantially all of the sample material within the second tubular structure 10. The system of embodiment 9, wherein the system is positioned to be open and directed toward the second target. (Aspect 17) The cross-sectional shape of the first tubular structure is one of a circle, an ellipse, or a polygon; The system according to embodiment 9. (Aspect 18) 10. The system of embodiment 9, wherein the first and second tubular structures are parallel to one another.

Claims

1. 1. A hood for improving deposition of sample material onto a target in a sample analysis system, comprising: a body including a top panel, a front panel, a left panel, a right panel, and a back panel, the top panel including a first opening configured to receive the sample material; a lower panel positioned adjacent to the rear panel, parallel to the upper panel and perpendicular to the rear panel, the lower panel including a second opening for receiving a dye or colorant after deposition of the sample material on the target; a bottom rim supporting the body and the lower panel, the bottom rim configured to adhere to the target prior to deposition; The hood, wherein the target comprises a glass slide, a coverslip, a plastic substrate, a charged cytology slide, or a coated cytology slide.

2. The hood of claim 1 , wherein the first opening is elevated above the top panel and the top panel extends beyond the front panel.

3. The hood of claim 2 , wherein a portion of the top panel extending beyond the front panel comprises a holder portion comprising one or more recesses.

4. The hood of claim 1 , wherein the rear panel includes a recessed notch positioned below the second opening configured to securely attach the hood to the sample analysis system.

5. The hood of claim 4 , wherein the body includes a convex notch configured to securely attach the hood to the sample analysis system.

6. The hood of any one of claims 1 to 5, wherein the bottom rim comprises a clamp configured to improve the seal between the bottom rim and the target.

7. The hood of any one of claims 1 to 5, wherein the first opening and / or the second opening are rectangular, circular, oval, or polygonal.

8. The hood of any one of claims 1 to 5, wherein the hood is manufactured using at least one of acrylic, polypropylene, polystyrene, styrene acrylonitrile, polycarbonate, or cyclic olefin copolymer.

9. The hood of any one of claims 1 to 5, wherein the bottom rim comprises a clamp configured to be depressed when the bottom rim contacts the target.

10. 1. A system for analyzing a sample, comprising: a gas nozzle configured to discharge gas toward a target comprising a glass slide, a coverslip, a plastic substrate, a charged cytology slide, or a coated cytology slide; a target holder arranged to hold the target; a sample dispenser for dispensing a liquid-based sample material onto the target; a body comprising a top panel, a front panel, a left panel, a right panel, and a back panel, the top panel comprising a first opening configured to receive the liquid-based sample material; a lower panel positioned adjacent to the rear panel, parallel to the upper panel and perpendicular to the rear panel, the lower panel including a second opening for receiving a dye or colorant after deposition of the liquid-based sample material on the target; and a hood comprising a bottom rim supporting the body and the lower panel, the bottom rim configured to adhere to the target prior to deposition; The system wherein the nozzle of the sample dispenser is positioned directly above the first opening.

11. The system of claim 10 , wherein the first opening is elevated above the top panel and the top panel extends beyond the front panel.

12. The system of claim 11 , wherein a portion of the top panel extending beyond the front panel comprises a holder portion comprising one or more recesses.

13. The system of claim 10 , wherein the rear panel comprises a recessed notch positioned below the second opening configured to securely attach the hood to a sample analysis system.

14. The system of claim 13 , wherein the body comprises a convex notch configured to securely attach the hood to the sample analysis system.

15. The system of any one of claims 10 to 14, wherein the bottom rim comprises a clamp configured to improve a seal between the bottom rim and the target.

16. The system according to any one of claims 10 to 14, wherein the first opening and / or the second opening are rectangular, circular, elliptical or polygonal.

17. The system of any one of claims 10 to 14, wherein the hood is manufactured using at least one of acrylic, polypropylene, polystyrene, styrene acrylonitrile, polycarbonate, or cyclic olefin copolymer.

18. The system of any one of claims 10 to 14, wherein the bottom rim comprises a clamp configured to be depressed when the bottom rim contacts the target.

19. The system of any one of claims 10 to 14, wherein the hood is replaceable.

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