Ultraviolet treatment evaluation device and ultraviolet treatment evaluation method

Abstract

To provide an ultraviolet treatment evaluation device which can easily and accurately evaluate an ultraviolet treatment effect on matters having a desired ultraviolet sensitivity by a water passage-type ultraviolet treatment device.SOLUTION: An ultraviolet treatment evaluation device 30A includes a detection number acquisition unit 32, a detection rate calculation unit 33, and an evaluation information production unit 34. The detection number acquisition unit 32 passes sample water through an ultraviolet treatment device 10, the sample water containing a synthetic DNA suspended therein which is produced so as to have a number of nucleotide sequences to be denatured by reacting to ultraviolet irradiation, the number corresponding to a predetermined reaction speed for the ultraviolet irradiation, and then acquires the detected number of the nucleotide sequences before denaturation. The detection rate calculation unit 33 calculates a detection rate of the nucleotide sequences in the sample water after passing the water through the ultraviolet treatment device 10 on the basis of the number of the nucleotide sequences in the sample water before doing so. The evaluation information production unit 34 produces, on the basis of the detection rate of the calculated nucleotide sequences, evaluation information on evaluation of an ultraviolet treatment effect on matters having the predetermined reaction speed for the ultraviolet irradiation by the ultraviolet treatment device 10.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an ultraviolet treatment evaluation device and an ultraviolet treatment evaluation method. [Background technology]

[0002] Conventionally, ultraviolet treatment devices that use ultraviolet light to sterilize microorganisms have been widely used. The average ultraviolet treatment effect of an ultraviolet treatment device can be evaluated by passing a water sample containing microorganisms known to be resistant to ultraviolet light through the device and measuring the inactivation rate (e.g., Non-Patent Document 1).

[0003] In addition, a technology has been developed that uses multiple types of microorganisms to obtain not only the average ultraviolet treatment effect of an ultraviolet treatment device, but also the validity of estimated information on ultraviolet irradiation distribution (for example, Patent Document 1). [Prior art documents] [Non-patent literature]

[0004] [Non-Patent Document 1] (Sommer et al.(2008)Disinfection of drinking water by UV irradiation: Basic principles - Specific requirements - International implementations, Ozone Science & Engineering, 30(1), 43-48) [Patent documents]

[0005] [Patent Document 1] Patent No. 6397362 [Patent Document 2] International Publication No. 2007 / 108332 Summary of the Invention [Problem to be solved by the invention]

[0006] As described above, a method for evaluating the average UV treatment effect of a UV treatment device using microorganisms has been established and there are many practical examples of this method. However, since the UV resistance of known microorganisms is limited, there has been a problem in that it is not possible to evaluate the UV treatment effect for microorganisms with UV resistance different from these microorganisms.

[0007] In addition, after passing sample water containing microorganisms through an ultraviolet treatment device, it takes more than a full day to measure the inactivation rate of the microorganisms, which poses the problem of taking a long time to generate evaluation information on the effectiveness of the ultraviolet treatment.

[0008] Another method for evaluating the average UV treatment effect of a UV treatment device has been developed, in which photochromic particles coated with a UV-reactive pigment are passed through a water-flow type UV treatment device and the amount of color change is measured to evaluate the UV treatment effect (e.g., Patent Document 2).

[0009] This method has the advantage that photochromic particles are easier to handle than microorganisms and the amount of discoloration of the particles can be measured quickly, making it easier and faster to perform the treatment than when using microorganisms to evaluate the average ultraviolet treatment effect of an ultraviolet treatment device.

[0010] However, the UV sensitivity of these photochromic particles is constant, and there was a problem in that it was not possible to evaluate the UV treatment effect depending on a substance having a different UV sensitivity from the photochromic particles, in other words, the UV treatment effect depending on a substance having a different UV resistance.

[0011] The present invention has been made in consideration of the above circumstances, and aims to provide an ultraviolet treatment evaluation device and an ultraviolet treatment evaluation method that can easily and accurately evaluate the ultraviolet treatment effect of a water-flow type ultraviolet treatment device on a substance with a desired ultraviolet sensitivity. [Means for solving the problem]

[0012] In order to solve the above-mentioned problems, the ultraviolet treatment evaluation device of the present invention includes a detection number acquisition unit that acquires the number of base sequences detected before denaturation after passing a water sample, in which synthetic DNA generated to contain a number of base sequences that denature in response to ultraviolet irradiation corresponding to a predetermined reaction rate to ultraviolet irradiation, through a water flow-through type ultraviolet treatment device; a detection rate calculation unit that calculates the detection rate of the base sequences, which is indicated by the ratio of the number of base sequences before denaturation detected in the water sample after passing the water through the water flow-through type ultraviolet treatment device, acquired by the detection number acquisition unit, to the number of base sequences contained in the water sample before passing the water through the water flow-through type ultraviolet treatment device; and an evaluation information generation unit that generates evaluation information evaluating the ultraviolet treatment effect of the water flow-through type ultraviolet treatment device on a substance that has the predetermined reaction rate to ultraviolet irradiation, based on the detection rate of the base sequences calculated by the detection rate calculation unit. Equipped with.

[0013] In addition, the ultraviolet treatment evaluation method of the present invention involves passing a sample of water containing suspended synthetic DNA, which has been produced to contain a number of base sequences that denature in response to ultraviolet irradiation corresponding to a predetermined reaction rate to ultraviolet irradiation, through a water-flow type ultraviolet treatment device, obtaining the number of base sequences detected before denaturation, calculating the detection rate of the base sequences, which is expressed as the ratio of the number of base sequences before denaturation detected in the sample of water after passing the water through the water-flow type ultraviolet treatment device to the number of base sequences contained in the sample of water before passing the water through the water-flow type ultraviolet treatment device, and generating evaluation information that evaluates the ultraviolet treatment effect of the water-flow type ultraviolet treatment device on a substance that has the predetermined reaction rate to ultraviolet irradiation based on the calculated detection rate of the base sequences. [Effects of the Invention]

[0014] According to the ultraviolet treatment evaluation device and ultraviolet treatment evaluation method of the present invention, the ultraviolet treatment effect of a water-flow type ultraviolet treatment device on a substance having a desired ultraviolet sensitivity can be evaluated simply and accurately. [Brief explanation of the drawings]

[0015] [Figure 1] 1 is a block diagram showing the configuration of an evaluation system using an ultraviolet treatment evaluation device according to an embodiment of the present invention. [Figure 2] This graph shows the results of irradiating sample water containing synthetic DNA containing thymine-thymine consecutive pairs with ultraviolet light, and then measuring the number of thymine-thymine consecutive pairs detected in the sample water using PCR for each ultraviolet light exposure dose and for each initial (before irradiation) thymine-thymine consecutive logarithm. [Figure 3] 1 is a graph showing the reaction rate constant for ultraviolet irradiation for each number of consecutive thymine-thymine pairs in synthetic DNA. [Figure 4] 3 is a flowchart showing the operation of the ultraviolet treatment evaluation device according to the embodiment of the present invention. [Figure 5] FIG. 10 is a block diagram showing the configuration of an evaluation system using an ultraviolet treatment evaluation device according to another embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0016] As one embodiment of the present invention, an ultraviolet treatment evaluation device for evaluating the ultraviolet treatment effect of an ultraviolet treatment device will be described below.

[0017] <Configuration of evaluation system using ultraviolet treatment evaluation device according to one embodiment> 1 is a block diagram showing the configuration of an evaluation system 1A using an ultraviolet treatment evaluation device according to this embodiment. The evaluation system 1A according to this embodiment includes an ultraviolet treatment device 10, a detection device 20, and an ultraviolet treatment evaluation device 30A.

[0018] The ultraviolet treatment device 10 is a water-flow type ultraviolet treatment device, and includes an ultraviolet irradiation tank 11 and an ultraviolet lamp 12 installed in the ultraviolet irradiation tank 11. The sample water to be treated flows into the ultraviolet irradiation tank 11 through an irradiation tank inlet 111, passes through the tank while being irradiated with ultraviolet light from the ultraviolet lamp 12, and flows out from an irradiation tank outlet 112.

[0019] In this embodiment, sample water containing suspended synthetic DNA is used as the sample water to be passed through the ultraviolet irradiation tank 11. The synthetic DNA to be added to the sample water is prepared in advance so as to contain a number of base sequences that denature in response to ultraviolet irradiation, the number corresponding to a predetermined reaction rate to ultraviolet irradiation. Details of the synthetic DNA to be added to the sample water will be described later.

[0020] The detection device 20 detects a predetermined base sequence from the sample water passed through the ultraviolet treatment device 10 using the polymerase chain reaction (PCR) technique.

[0021] The ultraviolet treatment evaluation device 30A includes a water sample information acquisition unit 31, a detection number acquisition unit 32, a detection rate calculation unit 33, an evaluation information generation unit , and an output unit .

[0022] The sample water information acquisition unit 31 acquires information about the sample water to be passed through the ultraviolet treatment device 10, such as the number of specific base sequences in the synthetic DNA suspended in the sample water and the reaction rate of the base sequences to ultraviolet irradiation.

[0023] The detection number acquisition unit 32 acquires the number of predetermined base sequences detected from the water sample by the detection device 20. The detection rate calculation unit 33 calculates the detection rate of the base sequence, which is indicated as the ratio of the number of the base sequences acquired by the detection number acquisition unit 32 to the number of the predetermined base sequences acquired by the water sample information acquisition unit 31.

[0024] The evaluation information generation unit 34 generates evaluation information that evaluates the ultraviolet treatment effect of the ultraviolet treatment device 10 on a substance that has a predetermined reaction rate to ultraviolet irradiation, based on the detection rate of a predetermined base sequence calculated by the detection rate calculation unit 33.

[0025] The output unit 35 is configured by, for example, a display device, and outputs the evaluation information generated by the evaluation information generating unit 34.

[0026] <Operation of evaluation system using ultraviolet treatment evaluation device according to one embodiment> The ultraviolet treatment effect of the ultraviolet treatment device 10 is generally evaluated by passing a water sample containing microorganisms whose ultraviolet resistance is known through the device and measuring the inactivation rate. However, since the number of microorganisms whose ultraviolet resistance is known is limited, it is not possible to evaluate the ultraviolet treatment effect of the ultraviolet treatment device 10 on microorganisms whose ultraviolet resistance is different from that of these microorganisms.

[0027] Therefore, in this embodiment, sample water in which pre-prepared synthetic DNA is suspended is used as the sample water passed through the ultraviolet treatment device 10 to evaluate the ultraviolet treatment effect of the ultraviolet treatment device 10. The synthetic DNA contained in the sample water is prepared so as to contain a number of base sequences that denature in response to ultraviolet irradiation corresponding to a predetermined reaction rate to ultraviolet irradiation. In this embodiment, a thymine-thymine sequence, which is a consecutive thymine base sequence, is used as the base sequence that denatures in response to ultraviolet irradiation.

[0028] When irradiated with ultraviolet light, thymine-thymine repeat pairs form dimers through an irreversible reaction. It has been experimentally confirmed that the reaction rate of thymine-thymine repeat pairs upon ultraviolet irradiation (UV sensitivity) depends on the number of thymine-thymine repeat pairs in the synthetic DNA of the sample water being treated.

[0029] Thymine-thymine repeat pairs can be detected by PCR-based techniques, but become undetectable once dimers are formed in response to UV irradiation. Therefore, the extent to which synthetic DNA containing a predetermined number of thymine-thymine repeat pairs has been exposed to UV irradiation can be measured by detecting the number of thymine-thymine repeat pairs in the synthetic DNA after UV irradiation using PCR.

[0030] Figure 2 is a graph showing the results of UV irradiation of a water sample containing synthetic DNA containing thymine-thymine repeat pairs, followed by PCR measurement of the number of thymine-thymine repeat pairs detected for each UV irradiation dose and for each initial (before UV irradiation) thymine-thymine repeat logarithm. In the graph, the dotted line (●) represents the measurement result when the number of thymine-thymine repeat logarithms before UV irradiation was the highest, while the dots (● → ◯ → ■ → □) represent the measurement results when the number of thymine-thymine repeat logarithms before UV irradiation was decreased.

[0031] As shown in Figure 2, the greater the UV dose, the fewer thymine-thymine continuous logarithms detected in the water sample after UV irradiation. Also, the greater the thymine-thymine continuous logarithms before UV irradiation, the greater the thymine-thymine continuous logarithms detected in the water sample after UV irradiation.

[0032] In this way, by taking advantage of the fact that a predetermined number of consecutive thymine-thymine pairs react at a predetermined rate to a predetermined amount of UV radiation, it is possible to generate synthetic DNA with any UV sensitivity, i.e., any reaction rate constant to UV radiation.

[0033] Figure 3 is a graph showing the reaction rate constant for UV irradiation for each number of thymine-thymine consecutive pairs in synthetic DNA, calculated based on the measurement results shown in Figure 2. By using information calculated in this way, instead of a microorganism having a predetermined reaction rate constant for UV irradiation, it is possible to evaluate the effectiveness of UV treatment by UV treatment device 10 by using synthetic DNA generated by including a predetermined number of thymine-thymine consecutive pairs so as to have a reaction rate constant similar to that of the microorganism. If the reaction rate constant of the synthetic DNA for UV irradiation is within a predetermined range from the reaction rate constant of the microorganism, for example, it can be determined to be similar to the reaction rate constant of the microorganism.

[0034] A process for generating evaluation information for evaluating the ultraviolet treatment effect by the ultraviolet treatment device 10 using synthetic DNA containing thymine-thymine consecutive pairs will be described below. Fig. 4 is a flowchart showing the process executed by the ultraviolet treatment evaluation device 30A when generating evaluation information for evaluating the ultraviolet treatment effect by the ultraviolet treatment device 10.

[0035] First, the user generates multiple synthetic DNAs with different numbers of thymine-thymine repeat pairs so that they react differently to UV irradiation. Each of these multiple synthetic DNAs has a primer sequence different from the other synthetic DNAs, which is used to detect the thymine-thymine repeat pairs by PCR.

[0036] The user prepares a water sample in which the generated synthetic DNAs are suspended. The user inputs information about the prepared water sample, specifically, the number of thymine-thymine consecutive pairs for each synthetic DNA suspended in the water sample and information about the reaction rate to ultraviolet irradiation, into the ultraviolet treatment evaluation device 30A. In the ultraviolet treatment evaluation device 30A, the input information is acquired by the water sample information acquisition unit 31 (S1).

[0037] A user introduces prepared sample water into the ultraviolet irradiation tank 11 through the irradiation tank inlet 111. The sample water passes through the ultraviolet irradiation tank 11 while being irradiated by the ultraviolet lamp 12, and then flows out from the irradiation tank outlet 112.

[0038] The detection device 20 detects thymine-thymine consecutive pairs based on the primers for each synthetic DNA from the sample water passed through the ultraviolet treatment device 10, and outputs the number of detected synthetic DNAs to the ultraviolet treatment evaluation device 30 A. At this time, the detection device 20 performs the detection process on all of the synthetic DNAs in the sample water.

[0039] In the ultraviolet treatment evaluation device 30A, the detection number acquisition unit 32 acquires the number of detected thymine-thymine consecutive pairs for each synthetic DNA output from the detection device 20 (S2). Next, the detection rate calculation unit 33 calculates the detection rate of thymine-thymine consecutive pairs for each synthetic DNA, which is indicated as the ratio of the number of thymine-thymine consecutive pairs of the corresponding synthetic DNA acquired by the detection number acquisition unit 32 to the number of thymine-thymine consecutive pairs for each synthetic DNA acquired by the sample water information acquisition unit 31.

[0040] The evaluation information generating unit 34 generates evaluation information that evaluates the ultraviolet treatment effect of the ultraviolet treatment device 10 on each substance having a reaction rate to ultraviolet irradiation equivalent to the reaction rate to ultraviolet irradiation of each of the multiple synthetic DNAs suspended in the sample water, based on the detection rate of thymine-thymine consecutive pairs for each synthetic DNA calculated by the detection rate calculating unit 33. If the reaction rate of the synthetic DNA to ultraviolet irradiation is within a predetermined range from the reaction rate of a predetermined substance to ultraviolet irradiation, it can be determined that the reaction rate is equivalent to the reaction rate of the substance.

[0041] The output unit 35 outputs the evaluation information for each reaction rate to ultraviolet irradiation generated by the evaluation information generating unit 34 (S4).

[0042] According to the first embodiment described above, by using sample water in which synthetic DNA containing a predetermined number of predetermined base sequences is suspended instead of microorganisms, the ultraviolet treatment effect of an ultraviolet treatment device can be easily and accurately evaluated.

[0043] PCR detection processing is generally used to detect specific base sequences from viruses. In this case, the number of bases that can be processed in one PCR detection processing is about several hundred, but since most viruses contain several thousand bases, it is not possible to process all of them, so the detection processing is performed on only a portion of the virus. This results in a decrease in detection accuracy.

[0044] In contrast, the synthetic DNA used in the above-described embodiment is generated with approximately 100 to 200 bases, so the entire synthetic DNA can be subjected to PCR detection processing, and highly accurate detection results can be obtained. This allows the ultraviolet treatment evaluation device to generate highly accurate evaluation information.

[0045] Furthermore, when evaluating the effectiveness of ultraviolet treatment by an ultraviolet treatment device using a water sample containing microorganisms using a conventional method, it takes more than 24 hours to measure the concentration of microorganisms in the water sample. In contrast, as described in the above embodiment, when evaluating the effectiveness of ultraviolet treatment by an ultraviolet treatment device using a water sample in which synthetic DNA is suspended, it takes approximately 2 to 3 hours to detect a predetermined base sequence from the water sample using PCR, including the required pretreatment. Therefore, the evaluation process of ultraviolet treatment effectiveness using the ultraviolet treatment evaluation device 30A according to this embodiment can significantly reduce the processing time compared to conventional methods.

[0046] Furthermore, conventional evaluation methods using microorganisms are limited in the types of microorganisms that can be used as evaluation targets, and evaluation information cannot be generated for microorganisms with different UV sensitivity than these microorganisms. In contrast, as described in the above-described embodiment, when evaluating the UV treatment effect of a UV treatment device using sample water in which synthetic DNA is suspended, it is possible to generate synthetic DNA with any UV sensitivity by adjusting the number of thymine-thymine consecutive pairs contained in the synthetic DNA and perform evaluation processing using this synthetic DNA. As a result, the processing method using the UV treatment evaluation device 30A of this embodiment can significantly expand the range of UV sensitivity of substances that can be evaluated for the UV treatment effect of the UV treatment device compared to conventional methods, thereby enabling highly accurate evaluation processing.

[0047] In the above-described embodiment, the base sequence of the primer portion is different for each synthetic DNA suspended in the water sample, so even if multiple synthetic DNAs with different UV sensitivities are suspended in one water sample, the detection device 20 can detect the number of thymine-thymine consecutive pairs for each synthetic DNA by PCR. This allows the ultraviolet treatment evaluation device 30A to generate evaluation information on the ultraviolet treatment effect of the ultraviolet treatment device for each synthetic DNA with different UV sensitivities through simple processing.

[0048] 5, an evaluation system 1B may be configured that includes an ultraviolet treatment evaluation device 30B. The ultraviolet treatment evaluation device 30B further includes an irradiation dose distribution estimation information acquisition unit 36, a reaction rate estimation value calculation unit 37, and a validity evaluation unit 38 in addition to the configuration of the ultraviolet treatment evaluation device 30A.

[0049] The irradiation dose distribution estimation information acquisition unit 36 ​​acquires estimated information on the ultraviolet irradiation dose distribution within the ultraviolet irradiation tank 11 when ultraviolet rays are irradiated onto the water to be treated by the ultraviolet treatment device 10, which information is generated from an irradiation dose distribution calculation program for ultraviolet irradiation that is pre-installed in the ultraviolet treatment device 10 and fluid analysis information using CFD.

[0050] The reaction rate estimation value calculation unit 37 calculates an estimate of the average reaction rate of a substance to ultraviolet light when ultraviolet light is irradiated on sample water containing the substance having a predetermined reaction rate to ultraviolet light irradiation by the ultraviolet treatment device 10, based on the estimated information of the ultraviolet light irradiation dose distribution acquired by the irradiation dose distribution estimation information acquisition unit 36.

[0051] The validity evaluation unit 38 generates validity judgment information that judges the validity of the estimated information of the ultraviolet irradiation dose distribution information acquired by the irradiation dose distribution estimation information acquisition unit 36 ​​based on whether the estimated value of the reaction rate calculated by the reaction rate estimation value calculation unit 37 and the reaction rate based on the detection rate of thymine-thymine consecutive pairs calculated by the detection rate calculation unit 33 are within a predetermined range and are close to each other.

[0052] By further including such a functional unit, the ultraviolet treatment evaluation device 30B can use the detection rate of thymine-thymine consecutive pairs to determine the validity of the ultraviolet irradiation dose distribution in the ultraviolet irradiation tank 11 in the ultraviolet treatment device 10 estimated by simulation. [Explanation of symbols]

[0053] 1A, 1B evaluation system 10. Ultraviolet treatment equipment 11 Ultraviolet irradiation tank 12 UV lamp 20 Detection device 30A, 30B UV treatment evaluation equipment 31 Sample water information acquisition unit 32 Detection number acquisition unit 33 Detection rate calculation section 34 Evaluation information generation unit 35 Output section 36 Irradiation dose distribution estimation information acquisition unit 37 Response rate estimate calculation section 38 Validity Assessment Section 111 Irradiation tank entrance 112 Irradiation tank outlet

Claims

1. a detection number acquisition unit that acquires the number of detected base sequences before denaturation after passing a water sample containing suspended synthetic DNA, the synthetic DNA being generated so as to contain a base sequence that denatures in response to ultraviolet irradiation for a number of minutes corresponding to a predetermined reaction rate to ultraviolet irradiation, through a water-passing type ultraviolet treatment device; a detection rate calculation unit that calculates a detection rate of the base sequences, which is indicated by the ratio of the number of the base sequences before denaturation detected from the water sample after passing the water through the water-passing type ultraviolet treatment device, acquired by the detection number acquisition unit, to the number of the base sequences contained in the water sample before passing the water through the water-passing type ultraviolet treatment device; an evaluation information generating unit that generates evaluation information that evaluates the ultraviolet treatment effect of the water-flow type ultraviolet treatment device on a substance having the predetermined reaction rate to ultraviolet irradiation, based on the detection rate of the base sequence calculated by the detection rate calculating unit; An ultraviolet treatment evaluation device equipped with:

2. 2. The ultraviolet treatment evaluation device according to claim 1, wherein the base sequence that denatures in response to ultraviolet irradiation is a thymine-thymine sequence.

3. The ultraviolet treatment evaluation device according to claim 1 , wherein the number of the base sequences before denaturation acquired by the detection number acquisition unit is a value detected using a polymerase chain reaction.

4. the detection number acquisition unit passes a water sample in which a plurality of synthetic DNAs each having a different number of base sequences before denaturation are suspended through the water-passing type ultraviolet treatment device, and then acquires the number of base sequences before denaturation detected for each of the plurality of synthetic DNAs; the detection rate calculation unit calculates a detection rate of the base sequence for each of the plurality of synthetic DNAs; 2. The ultraviolet treatment evaluation device according to claim 1, wherein the evaluation information generation unit generates evaluation information that evaluates the ultraviolet treatment effect of the water-flow type ultraviolet treatment device on each substance having a reaction rate to ultraviolet irradiation equivalent to the reaction rate to ultraviolet irradiation of each of the plurality of synthetic DNAs, based on the detection rate of the base sequence for each of the plurality of synthetic DNAs calculated by the detection rate calculation unit.

5. 5. The ultraviolet treatment evaluation device according to claim 4, wherein a primer for detecting the base sequence of each of the plurality of synthetic DNAs is generated with a base sequence different from that of other synthetic DNAs.

6. an irradiation dose distribution estimation information acquisition unit that acquires estimated information on the ultraviolet irradiation dose distribution in the water-through type ultraviolet treatment device when ultraviolet rays are irradiated on the treatment target water in the water-through type ultraviolet treatment device; a reaction rate estimation value calculation unit that calculates an estimate of the reaction rate of a substance to ultraviolet light when ultraviolet light is irradiated onto a water sample containing the substance having the predetermined reaction rate to ultraviolet light irradiation in the water-flow type ultraviolet treatment device, based on the estimated information of the ultraviolet light irradiation dose distribution acquired by the irradiation dose distribution estimation information acquisition unit; and a validity determination unit that generates validity determination information that determines the validity of the estimated information of the ultraviolet irradiation dose distribution information acquired by the irradiation dose distribution estimation information acquisition unit, based on whether or not the estimated value of the reaction rate calculated by the reaction rate estimation value calculation unit and the reaction rate based on the detection rate of the base sequence calculated by the detection rate calculation unit are within a predetermined range and are close to each other; The ultraviolet treatment evaluation device according to claim 1 , further comprising:

7. An ultraviolet treatment evaluation device, a water sample containing suspended synthetic DNA, the synthetic DNA being generated so as to contain a base sequence that denatures in response to ultraviolet irradiation, for a number of minutes corresponding to a predetermined reaction rate to ultraviolet irradiation, is passed through a water-passing ultraviolet treatment device, and then the number of base sequences detected before denaturation is obtained; calculating a detection rate of the base sequences, which is indicated by the ratio of the number of the base sequences before denaturation detected in the water sample after passing the water through the water-passing type ultraviolet treatment device to the number of the base sequences contained in the water sample before passing the water through the water-passing type ultraviolet treatment device; An ultraviolet treatment evaluation method that generates evaluation information that evaluates the ultraviolet treatment effect of the water-flow type ultraviolet treatment device on a substance that has the specified reaction rate to ultraviolet irradiation based on the calculated detection rate of the base sequence.

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