Tissue-catalyzed growth of polymers as therapeutic epithelial linings.

Abstract

The present disclosure provides compositions, methods, and kits that allow for in situ growth of polymers on or within a subject. In some aspects, the monomer dopamine polymerizes in vivo to form the polymer on tissue. In additional aspects, the compositions, methods, and kits are useful for treating or preventing diseases or disorders.

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application No. USSN 62 / 947,582, filed December 13, 2019, U.S. Provisional Patent Application No. USSN 63 / 050,206, filed July 10, 2020, and U.S. Provisional Patent Application No. USSN 63 / 050,216, filed July 10, 2020, the contents of each of which are incorporated herein by reference in their entirety.

[0002] Government support This invention was made with government support under Grant No. R01EB000244 awarded by the National Institutes of Health. The government has certain rights in this invention. [Background technology]

[0003] Background of the Invention The small intestine is a versatile organ with multiple physiological functions. The epithelium lining the gastrointestinal tract is a versatile tissue that plays essential roles as a permeable barrier for selective transport (e.g., absorption) and as a protective armor against various pathogens. Among other things, the epithelial tissue of the gastrointestinal tract, particularly the small intestinal epithelium, is not only a protective shield against physical abrasion, chemical stress, and pathogens, but also a dynamic lining for signal sensing, molecular transport, and immune regulation. Selective intervention of the small intestinal mucosa is relevant for disease treatment and health management. In parallel, a wide variety of carefully designed biotechnologies have been developed to address the challenge of specifically and efficiently restoring or enhancing the function of the small intestinal epithelium. These specific technologies utilize either elaborately engineered tissue adhesives (e.g., pH-dependent polymers, metal complexes, and targeted nanoparticles) or systematically engineered tissue substitutes (e.g., epithelial grafts, autologous cell sheets, and plastic epithelial sleeves) and are effective tools for facilitating the restoration of epithelial dysfunction and the treatment of systemic diseases. 1~7Despite these advances in the laboratory, widespread adoption of these technologies in medical laboratories and health care clinics has been limited, thereby stifling their impact. 8~10 This limited practical application is the result of multiple factors: invasive implantation, potential immunogenicity, toxicity, and the imprecision, instability, and inconvenience of current tissue-targeting strategies, which limit selective small bowel access.

[0004] Similarly, the potential for intervention in the small intestine to treat digestive and systemic diseases has intrigued scientists to develop a variety of targeted drug therapies. 31、32 However, challenges with small intestine-specific targeting have inhibited widespread adoption. 9、10 Alternative technologies, such as intestinal sleeves, which require surgical implantation, are limited to treatments with complex procedures, poor biocompatibility, risk of inflammation, and high costs. Therefore, evolving the function of the intraepithelial lining through advanced biotechnology while maintaining the physiological properties of the tissue remains a challenge. Summary of the Invention

[0005] SUMMARY OF THE INVENTION Disclosed herein are compositions, methods, and kits for forming polymers in situ in a subject. The present disclosure provides a transient coating layer with tunable functionality, allowing for the growth of polymers on the surface of epithelial tissue. The polymeric coating relies on dopamine polymerization catalyzed by an endogenous cellular enzyme (catalase), strong tissue adhesion created through chemical crosslinking, and, optionally, functional agents incorporated through simple conjugation. For example, catalase in epithelial tissue in the gastrointestinal epithelium catalyzes the growth of polydopamine on the small intestinal mucosa. Additionally, catalase expression levels along tissues, such as the gastrointestinal tract, allow for efficient and specific formation of polymeric coatings.

[0006] The disclosed compositions, methods, and kits are useful, for example, in enhancing lactose digestion by immobilizing galactosidase in the intestine, leading to improved lactose digestion; regulating nutrient intake by interfering with glucose absorption; and controlling drug delivery through extending the residence time of active pharmaceutical ingredients in specific anatomical locations.

[0007] In one aspect, provided herein is a method for forming a polymer in situ in a subject, comprising administering to the subject a composition comprising a monomer and an oxygen source, wherein the monomer and oxygen source contact a catalyst endogenous to the subject, and the catalyst polymerizes the monomer, wherein the monomer is dopamine or a salt thereof, the oxygen source is hydrogen peroxide or urea hydrogen peroxide, and the endogenous catalyst is selected from catalase or peroxidase.

[0008] In one aspect, the present disclosure provides a composition comprising dopamine, an oxygen source, and optionally a buffering agent. In some aspects, the composition further comprises a digestive enzyme, a nutrient blocker, a dietary supplement, a radioprotectant, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof.

[0009] In another aspect, the present disclosure provides a method of treating a disease or disorder, comprising administering to a subject in need thereof an effective amount of a composition as described herein.

[0010] In one aspect, the present disclosure provides a method of preventing a disease or disorder, comprising administering to a subject in need thereof an effective amount of a composition as described herein.

[0011] In a further aspect, the present disclosure also provides a kit comprising a composition as described herein and instructions for administering it.

[0012] Details of certain aspects of the present disclosure are set forth in the "Detailed Description of Certain Aspects" section below. Other features, objects, and advantages of the present disclosure will become apparent from the definitions, examples, and claims. Detailed Description of the Drawings [Brief explanation of the drawings]

[0013] [Figure 1]Figures 1A-1H. Endogenous enzyme-catalyzed polydopamine growth on small intestinal epithelium. Figure 1A shows a schematic illustration of the tissue-accelerated polymerization (tissue-accelerated polymerization) of the present disclosure. Dopamine monomers in oral monomer solution are rapidly oxidized in the presence of hydrogen peroxide (HO) under endogenous catalase catalysis and form a polydopamine coating on the small intestinal epithelium. This specific small intestinal coating and targeting is achieved due to the heterogeneous distribution of catalase along the gastrointestinal tract. Figure 1B shows a schematic illustration of catalase-accelerated polydopamine polymerization in a hypoxic environment. As depicted above the horizontal arrow, in an extremely oxygen-deficient environment, oxidation of dopamine (colorless) (using oxygen as the oxidant) and the formation of polydopamine (dark brown) were inhibited and nearly quenched, even in the presence of HO. In contrast, catalase can boost oxygen release (using H2O2 as the oxygen source) and accelerate polydopamine polymerization, for example, approximately 400 times. Thus, as shown below the horizontal arrow, colorless dopamine rapidly polymerizes into polydopamine in the presence of hydrogen peroxide and catalase. TAPPE = tissue-accelerated polymerization on the outer epithelium. Figure 1C shows visual observations of polydopamine polymerization under the conditions shown in Figure 1B at various time points. Figure 1D shows the attenuation measured at 700 nm for the sample shown in Figure 1C. The optical attenuation occurring in 20 seconds under catalase catalysis was the same as that occurring in 2 hours under other conditions. Figure 1E depicts images showing polydopamine coating on the mucosal and serosal sides of porcine small intestine after application of the ex vivo coating treatment as disclosed herein, with the scale bar representing 2 cm. Figure 1F shows a quantitative assessment of in situ ex vivo polymerization rates. The polydopamine signal reached completion within 12 minutes. Data were reported as the mean ± SD across three porcine tissue samples. Figure 1G depicts images showing porcine tissue samples in different parts of the gastrointestinal tract before and after tissue-accelerated polymerization coating. Samples (6 mm diameter) were collected at three sites of the polydopamine-coated tissue. Figure 1H shows quantitative measurements of polydopamine signal intensity for the samples shown in Figure 1G.The difference in intensity between the small intestine and other tissues is statistically significant. ****P<0.05, one-way analysis of variance (ANOVA) and post-hoc Bonferroni. Data are reported as mean ± SD across three different tissue samples.

[0014] [Figure 2]Figures 2A-2H. Biological mechanism of tissue-accelerated polymerization. Figure 2A shows an evaluation of the relationship between tissue catalytic capacity and polydopamine polymerization. Porcine tissue lysates were individually added to tissue-accelerated polymerization solutions, and the attenuation of each solution was measured at 700 nm. Both visual assessment (upper panel) and quantitative measurement (lower panel) of the polydopamine solutions demonstrate differences in catalytic capacity when comparing small intestinal epithelium with other tissues and controls (no lysate). *P<0.05, one-way ANOVA and post-hoc Bonferroni. Data are reported as mean ± SD across three replicates. Figures 2B-2C demonstrate validation of the exclusive role of catalase in the reaction shown in Figure 2A by treating lysates with either a catalase-specific inhibitor (Figure 2B) or an antibody that induces immunoprecipitation (Figure 2C). The difference in relative catalytic capacity between treated and untreated groups is visually and statistically significant. ***P<0.001 by two-tailed t-test. Data are reported as mean ± SD across three different tissue samples. Figures 2D–2F show quantification of catalase expression (gene and protein levels) along the porcine gastrointestinal tract by catalase activity analysis (Figure 2D), real-time PCR (Figure 2E), and Western blotting (Figure 2F). Similar distribution profiles are achieved in Figures 2D–2F, suggesting higher catalase expression levels in the small intestinal epithelium compared to other tissues. Differences are statistically significant. ***P<0.05, one-way ANOVA and post-hoc Bonferroni analysis. Data are reported as mean ± SD across three replicates. Figure 2G shows microscopic analysis of polydopamine-coated small intestinal epithelium, demonstrating a thin polydopamine layer on the external villi (represented by arrows) but none on the control tissue (uncoated). Scale bar, 150 µm. Figure 2H shows bright-field imaging of uncoated tissue slices treated with specific peroxisome / catalase staining (left panel) and with tissue-accelerating polymerization solution (right panel). Dark brown polydopamine spots are observed within the tips of the villi, and the staining pattern is consistent with conventional peroxisome / catalase staining. Scale bar, 150 μm.

[0015] [Figure 3]Figures 3A-3I. In vivo performance of tissue-accelerated polymerization coating in pigs. Figures 3A-3C show schematic diagrams and photographs of in vivo gastrointestinal endoscopic real-time recording of polydopamine formation during the tissue-accelerated polymerization process. The tissue-accelerated polymerization solution was administered directly into the pig's small intestine through a catheter under endoscopic visual guidance, and an endoscopic camera was placed both inside and outside the solution within the small intestine for observation. Figures 3B-3C show endoscopic images (bottom three panels) revealing steps during polydopamine coating. Oxygen bubbles were generated at the epithelium-solution interface (represented by white arrows). Figure 3D shows a schematic illustration of small intestinal ligation (top panel) and direct polydopamine coating evaluation (bottom panel). The tissue-accelerated polymerization solution filled the intestinal lumen up to the clamping site and was prevented from descending to the lower small intestine. Isolated tissue showed different polydopamine coatings before and after clamping. Figure 3E shows a schematic illustration of the intestinal retention of the polydopamine coating as revealed by X-ray images of the polydopamine probe. X-ray images were taken for two studies: (I) short-term stability assessment (rinsing the coated area) and (II) long-term retention assessment (24-hour liquid meal). PDA = polydopamine. Figure 3F shows X-ray images of the polydopamine probe and the polydopamine coating layer stably retained in the small intestine before and after rinsing the imaging area shown in study (I). The stomach and small intestine (SI) areas are separated by white dotted lines. The polydopamine coating is represented by yellow arrows. Figure 3G shows X-ray images of the intestinal retention of the polydopamine coating during the period of study (II). The conventional probe was nearly undetectable after exposure to food. Figure 3H shows quantitative signal intensity analysis of the region of interest (ROI), the coated small intestine area, in Figure 3F. The improved signal and stability (before and after rinsing) in the polydopamine probe compared to the conventional (CON) probe demonstrates efficient probe incorporation and stable polydopamine coating. Data are reported as mean ± SD over three separate measurements.Figure 3I shows quantitative signal intensity analysis of the polydopamine coating over time in Figure 3G. From 2 to 6 hours, there was only a 28% difference in signal intensity, indicating prolonged retention of the polydopamine coating. Data are reported as the mean ± SD across three separate measurements.

[0016] [Figure 4]Figures 4A-4G. Therapeutic applications enabled by tissue-accelerated polymerization. Figure 4A shows a schematic illustration of the tissue-accelerated polymerization therapeutic platform. Functional agents, including digestive enzymes, nutrient blockers, and antiparasitic drugs, were incorporated into the platform through oral co-administration with the tissue-accelerated polymerization solution to pigs. Figure 4B shows a schematic illustration of the incorporation of digestive enzymes (β-galactosidase, β-gal) into a polydopamine coating layer on porcine small intestinal epithelium to enhance substrate (lactose) digestion. After in vivo coating, β-gal activity of the coated epithelium was assessed. Figure 4C shows a quantitative comparison of β-gal activity in tissue-accelerated polymerization-based β-gal-coated tissue, which showed increased enzyme activity compared to negative control subjects. Data are reported as the mean ± SD across four animals. Figure 4D shows a schematic illustration of incorporating a nano-crosslinker (blocker) into the tissue-accelerated polymerization coating to create an impermeable polydopamine layer that prevents glucose uptake in the small intestine. After in vivo coating, pigs were administered oral glucose, followed by monitoring of blood glucose concentrations. Figure 4E shows a quantitative comparison of blood glucose changes in pigs with the tissue-accelerated polymerization coating, demonstrating a reduced glycemic response compared to the control (no coating). Data were averaged across animals (each animal represented by a gray line) in each group (indicated by a black line). Figure 4F shows a schematic illustration of coating drug (praziquantel) particles onto the small intestinal epithelium for sustained release of the therapeutic agent in the small intestine. After oral administration of the praziquantel tissue-accelerated polymerization solution, serum drug concentrations were analyzed over 48 hours. Figure 4G shows a quantitative comparison of pharmacokinetics, demonstrating an extended residence time of praziquantel (with tissue-accelerated polymerization solution) compared to the control (without tissue-accelerated polymerization solution). * denotes p<0.02, two-sample t-test comparing the praziquantel tissue-accelerated polymerization group with the control group at matched time points. Data are reported as the mean ± SD across three animals.

[0017] [Figure 5]Figures 5A-5G. Compatibility of human tissue with tissue-accelerated polymerization. Figure 5A shows images of freshly excised tissue specimens from human small intestine before and after ex vivo tissue-accelerated polymerization coating. A dark brown polydopamine coating was clearly observed on the surface of the human small intestine (bottom panel). Scale bar, 1 cm. Figure 5B depicts the coating kinetics showing the ultrafast development of polydopamine signal in ex vivo human tissue. The polydopamine signal reached completion within 3 minutes. Figure 5C shows an evaluation of the consistency of tissue-accelerated polymerization. Tissue specimens from four donors of different ages, races, and genders were tested. Five ex vivo evaluations were performed on random sites of the human small intestine. Quantitative measurements of the polydopamine coating signal in samples (6 mm diameter) before coating (right panel) and after coating (left panel) confirmed consistent tissue-accelerated polymerization coating performance. Data are reported as the mean ± SD across five replicates. Figure 5D shows microscopic and histological analysis of frozen (40 μm thick) and FFPE (5 μm thick) specimens collected from polydopamine-coated human small intestinal epithelium. A thin polydopamine layer was observed on the external villi of the coated tissue. Uncoated tissue was used as a control. Histological studies (hematoxylin and eosin (H&E) staining) of adjacent frozen tissue slides and FFPE samples showed that the epithelial layer remained intact with a staining pattern similar to that of the control, demonstrating the absence of tissue toxicity. Scale bar, 150 μm. Figure 5E depicts representative images showing that there was no obvious reduction in polydopamine signal after mechanical agitation and scratching in coated human small intestine. Figure 5F shows quantitative ex vivo assessment of polydopamine signal intensity in coated tissue under a range of physical conditions. Differences in intensity across conditions were not statistically significant (two-tailed t-test). Data are reported as mean ± SD across three replicates. Figure 5G shows quantitative ex vivo assessment of polydopamine signal intensity in coated tissue under a range of chemical conditions. Differences in intensity across conditions are not statistically significant (two-tailed t-test).Data are reported as the mean ± SD over three replicates.

[0018] [Figure 6] Figures 6A-6B. Comparison of polydopamine polymerization kinetics under different reaction conditions. Figure 6A shows the attenuation measured at 700 nm for samples undergoing polydopamine polymerization in air (with and without HO (control)). Figure 6B shows the attenuation measured at 700 nm for samples undergoing polydopamine polymerization in extremely low oxygen levels (hypoxic environment) (with and without HO (control)). At extremely low oxygen levels, polydopamine polymerization (without HO) was inhibited by 65% ​​compared to conventional conditions (reaction in air, without HO). The addition of HO nearly quenched polydopamine polymerization in air and hypoxic conditions.

[0019] [Figure 7] Figures 7A-7D. Fourier transform infrared (FTIR) spectra of dopamine, polydopamine standards, and polymerization products. FTIR spectra were measured for dopamine (Figure 7A) and polydopamine (Figure 7B) prepared under conventional conditions and used as standards. The FTIR spectra confirm polydopamine formation in the polymerization products catalyzed by catalase (commercially purified) (Figure 7C) and catalase (from tissue lysate) (Figure 7D). The indole (or indoline) peaks (1515 and 1605 cm-1) and the broad peak (hydroxyl structure) spanning 3200-3500 cm-1 in the catalase-catalyzed polymerization products are nearly identical to those in the polydopamine standards.

[0020] [Figure 8]Figures 8A-8C. Normalized attenuation spectra of polydopamine standard and polymerization product. The UV-vis spectra of the polydopamine standard (Figure 8A), the polymerization product catalyzed by catalase (commercially purified) (Figure 8B), and the polymerization product catalyzed by catalase (from tissue lysate) (Figure 8C) are nearly identical, confirming polydopamine formation in Figures 8B and 8C.

[0021] [Figure 9] Figure 9. Evaluation of tissue-accelerated polymerization coating using porcine tissue specimens. The mucosa and serosa of the small intestine (schematic illustration, left panel) were separately exposed to the tissue-accelerated polymerization solution, and a dark brown polydopamine coating was observed only on the mucosal side of the tissue (middle panel), but not on the serosal side of the tissue (right panel). To confirm the specificity of the coating, sutures were used to tie the two ends of the small intestinal tissue, and only the section between the sutures was exposed to the tissue-accelerated polymerization solution.

[0022] [Figure 10] Figure 10. Catalytic activity analysis of tissue lysates through native gel electrophoresis. Tissue lysates from different parts of the porcine gastrointestinal tract were loaded onto a non-denaturing polyacrylamide gel, electrophoresed to allow protein separation, and stained for catalytic activity analysis. After staining with tissue-accelerating polymerization solution, a dark brown polydopamine signal was visualized on the gel. Only one sharp band (represented by an arrow) was observed in each lane, supporting the predicted role of catalase as the sole enzyme involved in polydopamine polymerization.

[0023] [Figure 11]Figures 11A-11B. Quantification of catalase mRNA levels in tissues by using real-time PCR. Housekeeping genes, including β-actin (Figure 11A) and 18S (Figure 11B), were used as controls to quantify catalase mRNA levels. Tissue specimens from four pigs were tested. Small intestinal catalase mRNA expression levels had similar distribution profiles in Figures 11A and 11B. Data are reported as the mean ± SD across four animals.

[0024] [Figure 12] Figures 12A-12E. Ct values ​​for gene expression levels. For quantification of mRNA levels, catalase (Figure 12A), β-actin (Figure 12B), 18S (Figure 12C), GUS (Figure 12D), and GAPDH (Figure 12E) genes were included in the study. Tissue specimens from four pigs were tested. Data are reported as the mean ± SD across four animals.

[0025] [Figure 13] Figure 13. Bright-field image of small intestinal epithelium coated with polydopamine. Porcine small intestinal tissue was exposed ex vivo to the tissue-accelerating polymerization solution for 3 and 15 minutes and examined under a bright-field microscope. Polydopamine was first deposited on the tips of the intestinal villi (represented by yellow arrows, upper panel) and then coated the entire villi and surrounding areas (lower panel). Scale bar, 500 μm.

[0026] [Figure 14]14A-14C. Evaluation of in vivo tissue-accelerated polymerization coating performance in the stomach. FIG. 14A shows a schematic illustration of real-time gastrointestinal endoscopic recording of the stomach after oral administration of a tissue-accelerated polymerization solution to the stomach. The pig was moderately sedated throughout the entire process. An endoscopic camera was placed outside the solution for observation, and images were recorded at the same location over time. FIG. 14B shows endoscopic images revealing no visualization of the dark brown polydopamine in the stomach. The tissue-accelerated polymerization solution in the stomach remains clear for 20 minutes. FIG. 14C shows an image of an isolated stomach from the animal in FIG. 14B, confirming the absence of a polydopamine coating on the epithelial surface.

[0027] [Figure 15] Figures 15A-15C. Evaluation of the ex vivo tissue coating performance of polydopamine probes through X-ray imaging. Figure 15A shows images of conventional (CON) probe solutions and polydopamine probe solutions. The polydopamine probe was prepared by encapsulating the conventional probe with a thin layer of polydopamine. The dark brown color of the polydopamine probe solution comes from the chromogenic polydopamine on the probe surface. Figure 15B shows a schematic illustration of the ex vivo tissue coating process. A pig small intestine was placed in a Franz Cell, and tissue-accelerating polymerization solution (with or without a probe) was added into the chamber. After coating, the coated tissue was rinsed with water and imaged using an X-ray system. Figure 15C shows X-ray images of tissues coated using a polydopamine probe (left panel), tissue-accelerating polymerization solution with a conventional (CON) probe (center panel), and tissue-accelerating polymerization solution without a probe (right panel). A strong X-ray signal was observed only within the coated area (represented by the red circle) of the tissue coated with the polydopamine probe. No obvious X-ray signal was detected in the control tissues where either the conventional probe or polydopamine alone was applied for coating.

[0028] [Figure 16] Figures 16A-16B. In vivo X-ray imaging of the intestinal retention of polydopamine coating layers. Figure 16A shows X-ray images demonstrating the stability of the polydopamine coating. Healthy pigs were orally administered a tissue-accelerating polymerization solution (upper panel) containing a suspended polydopamine probe and a conventional probe (lower panel), respectively, and imaged using an X-ray system. The solution was administered directly into the small intestine through a catheter under endoscopic visual guidance. The same pig without the probe was imaged and used as a control. To test the stability of the polydopamine coating, water was used to rinse the imaging area. Figure 16B shows X-ray images demonstrating the intestinal retention of polydopamine over time. A series of X-ray images was taken periodically at the same location at 2, 6, and 24 hours. The animals were consistently fed a liquid diet throughout the imaging period, mimicking realistic conditions to test the stability of the polydopamine coating in the presence of food.

[0029] [Figure 17] Figures 17A-17B. Characterization of nano-crosslinkers. Figure 17A shows TEM images at different magnifications showing uniform polydopamine nano-crosslinkers. Figure 17B depicts dynamic light scattering (DLS) analysis showing the hydrodynamic size of the nano-crosslinkers at 527 nm.

[0030] [Figure 18]Figures 18A-18B. Evaluation of tissue-accelerated polymerization coating performance across different animal species. Samples (6 mm diameter) were collected at three to five random sites of polydopamine-coated tissue, and images of the samples were analyzed for quantification of polydopamine coating. ImageJ was used to identify regions of interest that encompassed the polydopamine-coated tissue and excluded areas without tissue, which were "blank." The same analysis was performed on all samples in each group to obtain the overall average polydopamine signal intensity and analyze signal variability. This is an ex vivo study. Figure 18A shows the quantitative assessment of polydopamine coating density on small intestines from pigs, humans, and rats. After tissue-accelerated polymerization coating, polydopamine signal intensity, an indication of polydopamine coating density, was measured. Data are reported as the mean ± SD across three replicates. Figure 18B shows the quantification of catalase expression by measuring catalase activity in the small intestines from pigs, humans, and rats. Tissue specimens were collected from the same animals as in Figure 18A. Data are reported as the mean ± SD of three replicates.

[0031] [Figure 19] Figure 19. Dose-dependent cytotoxicity of polydopamine in HeLa, COLO320DM, Caco-2, Hep3B, and HS 895.T cells. Cells were treated with polydopamine at various concentrations, and cytotoxicity was analyzed at different time points. Polydopamine was lowly toxic (>80% viability) in the concentration range of 0 to 2000 μg ml-1 to all cell lines after 24 h of polydopamine exposure.

[0032] [Figure 20]Figures 20A-20C. Weight change in rats during a 28-day oral toxicity evaluation. Rats were separately exposed to water (control) (Figure 20A), neat polydopamine (Figure 20B), and tissue-accelerating polymerization solution (Figure 12C) over a 4-week period. No significant differences in weight were observed between rats exposed to tissue-accelerating polymerization solution, polydopamine, and water. Data were averaged among animals (each animal is represented by a gray line) in each group (indicated by a black line). Data are reported as the mean ± SD across four animals.

[0033] [Figure 21] Figure 21. Hematological measurements of blood from rats after a 28-day oral toxicity evaluation. Blood samples were collected from rats exposed to water (control), neat polydopamine, and tissue-accelerated polymerization solution. No significant differences in hematological parameters were observed between rats exposed to tissue-accelerated polymerization solution, polydopamine, and water (four replicates).

[0034] [Figure 22] Figure 22. Blood biochemistry testing of blood from rats after 28 days of oral toxicity evaluation. Blood samples were collected from rats exposed to water (control), neat polydopamine, and tissue-accelerated polymerization solution. No significant differences were observed in blood biochemistry parameters between rats (4 replicates) exposed to tissue-accelerated polymerization solution, polydopamine, and water.

[0035] [Figure 23] Figure 23. Histology of major organs collected from rats after a 28-day oral toxicity evaluation. Tissues were collected from rats exposed separately to water (control), neat polydopamine, and tissue-accelerating polymerization solution. No noticeable organ damage was observed in mice treated with polydopamine and tissue-accelerating polymerization solution compared to controls. Scale bar, 300 μm.

[0036] [Figure 24]Figures 24A-24B. Evaluation of the performance of tissue-accelerated polymerization on epithelium after removal of bacteria present in small intestinal mucus. Samples (6 mm diameter) were collected at three to five random locations on the polydopamine-coated tissue, and images of the samples were analyzed for quantification of polydopamine coating. ImageJ was used to identify regions of interest that encompassed the polydopamine-coated tissue and excluded areas without tissue (blank). The same analysis was performed on all samples in each group to obtain the overall average polydopamine signal intensity and analyze signal variability. This is an ex vivo study. Figure 24A depicts images showing tissue samples (with and without bacterial removal) after ex vivo tissue-accelerated polymerization. Samples (6 mm diameter) were collected at three random locations on the polydopamine-coated tissue. Bacteria were removed from the luminal surface of the epithelium by incubation with an antibiotic-antimycotic solution and repeated washing. Figure 24B shows quantitative measurements of polydopamine signal intensity for the samples shown in Figure 24A. The difference in intensity is not statistically significant. P>0.05 by two-tailed t-test. Data are reported as the mean ± SD across three different tissue samples.

[0037] [Figure 25] Figure 25. Comparison of catalase catalytic capacity between epithelium and mucus (bacteria). A solution (180 μl) was first prepared and added into a 96-well plate, followed by the addition of 10 μl of tissue lysate (villi or mucus). The reaction solution was maintained at 37°C for 20 minutes. The attenuation of the solution at 700 nm was measured using a plate reader (Tecan). This is an ex vivo study. The relative catalytic capacity of intestinal villi (without bacteria) is higher than that of bacteria in mucus. Mucus was collected (3 cm2) on top of the epithelium, and epithelial villi were collected from the same tissue area. Samples were diluted to the same volume for measurement. The difference in capacity is statistically significant. ***P<0.001 by two-tailed t-test. Data are reported as the mean ± SD across three different replicates.

[0038] [Figure 26] Figures 26A-26C. Microscopic analysis of polydopamine epithelial deposition. Tissue (12 cm2) was exposed to tissue-accelerated polymerization solution (10 mL) for 20 minutes and then washed three times with PBS buffer (1x) to remove excess polydopamine. Removal of polydopamine: Polydopamine-coated small intestine was snap-frozen and embedded in optimal cutting temperature (OCT) compound. Fixed tissue was cut into 40 μm-thick sections using a cryostat (Leica Biosystems). This was an ex vivo study. Figure 26A shows bright-field imaging of a tissue slice coated with tissue-accelerated polymerization. A dark brown polydopamine layer was deposited only on the luminal surface of epithelial cells (indicated by arrows), but not inside the cells. Figure 26B shows a bright-field image of an uncoated tissue slice. The light yellow background signal comes from blood vessels. Figure 26C shows bright-field imaging of intracellular polydopamine deposition on a sectioned control tissue slice, in which both dopamine and hydrogen peroxide molecules were able to diffuse freely and rapidly into the epithelial cells. Dark brown polydopamine deposits (represented by arrows) were observed inside the epithelial cells but not on the surface of the cells, and the deposition pattern was significantly different compared to the tissue slice coated with tissue-accelerated polymerization. Scale bar, 150 μm.

[0039] [Figure 27] Figure 27. Polydopamine polymerization kinetics in luminal solution. Polydopamine polymerization kinetics was assessed in luminal solution located on porcine epithelium through ex vivo studies. Attenuation was measured at 700 nm for samples undergoing polydopamine polymerization. Polydopamine polymerization kinetics under tissue-accelerated polymerization (without epithelial catalysis) was used as a control. The optical attenuation occurring in 45 seconds under epithelial catalysis was the same as the attenuation occurring in 1 hour under control conditions.

[0040] [Figure 28]Figure 28. Evaluation of polydopamine signals in different cell fractions. Cell fractionation was performed on villi coated with tissue-accelerated polymerization. Villi were stripped from the luminal surface (longitudinal opening) of small intestinal tissue placed on an ice-cold substrate. Polydopamine signals were evaluated by measuring the attenuation of each cell fraction at 700 nm. No polydopamine signal was detected in the cytoplasmic fraction, but a clear polydopamine signal was detected in the membrane and other fractions. Differences in intensity are statistically significant. **P<0.01 by two-tailed t-test. Data are reported as mean ± SD across three replicates.

[0041] [Figure 29] Figure 29. Evaluation of the stability of tissue-accelerated polymerization in the stomach. To evaluate stability, tissue-accelerated polymerization solution (dopamine (500 mg) and HO (1 M, 1 mL) rapidly added to Tris buffer (50 mM, 50 mL) at pH 8.5 and used fresh) was administered to pigs (in vivo, with a non-disruptive clamp applied to the pylorus), and stomach samples were harvested at different time points and characterized through ex vivo coating studies. Relative coating performance showed that the harvested solutions (from 10 to 30 minutes) exhibited consistent coating performance, and the relative coating efficiency dropped by only 30% after 60 minutes, demonstrating that neither dopamine nor hydrogen peroxide during tissue-accelerated polymerization were absorbed or ingested in significant amounts in the stomach. Data are reported as the mean ± SD across three replicates.

[0042] [Figure 30]Figure 30. Evaluation of polydopamine signal in the submucosa. No detectable increase in polydopamine signal was observed in porcine submucosa after in vivo tissue-accelerated polymerization. The solution consisted of dopamine (500 mg) and HO (1 M, 1 mL). Both were rapidly added to Tris buffer (50 mM, 50 mL) at pH 8.5 and used fresh. Epithelial tissue (uncoated) was used as a control. The difference in signal was not statistically significant. P>0.05 by two-tailed t-test. Data are reported as mean ± SD over three replicates.

[0043] [Figure 31] Figures 31A-31B. Assessment of dopamine concentrations in blood and submucosa via liquid chromatography-tandem mass spectrometry. Figure 31A shows that no significant changes in dopamine concentrations in blood were observed after in vivo administration of tissue-accelerated polymerization solution (dopamine (500 mg) and HO (1 M, 1 mL) rapidly added to Tris buffer (50 mM, 50 mL) at pH 8.5 and used fresh). *P>0.05, two-sample t-test comparing the tissue-accelerated polymerization group with the control group (no tissue-accelerated polymerization) at matched time points. Data are reported as mean ± SD across three animals. Figure 31B shows that no detectable increase in dopamine concentrations was observed in the submucosa after administration of the tissue-accelerated polymerization solution (3 hours later). The difference in concentrations was not statistically significant. *P>0.05 by two-tailed t-test. Data are reported as mean ± SD across three replicates.

[0044] [Figure 32]Figures 32A-32D. Evaluation of the blocking efficiency of the tissue-accelerated polymerization layer through ex vivo studies. Tissues were exposed to a solution (dopamine (500 mg) and HO (1 M, 1 ml) rapidly added to Tris buffer (50 mM, 50 ml) at pH 8.5. The mixed solution was used fresh) and washed three times with PBS buffer (1x) to remove excess polydopamine. Different concentrations of polydopamine nano-crosslinker (25 mg / ml, 12.5 mg / ml, and 0 mg / ml) were suspended in the tissue-accelerated polymerization solution. This is an ex vivo study. Figures 32A-32C show the relative barrier function (tissue permeability) of the tissue-accelerated polymerization layer on Ca (Figure 32A), glutamate (Figure 32B), and glucose (Figure 32C). All three nutrients showed reduced tissue permeation in three separate experiments, with the tissue-accelerated polymerization barrier blocking approximately 49% of Ca2+, approximately 71% of glutamate, and approximately 78% of glucose. Figure 32D shows the relative barrier function (tissue permeation of glucose) of tissue-accelerated polymerization layers (with different crosslinking densities). When fewer nano-crosslinkers were incorporated into the coating layer, glucose had normal tissue permeation levels, demonstrating that the polydopamine coating layer itself did not affect cellular glucose absorption, but the crosslinking density of the coating layer regulated glucose absorption efficiency. Pig small intestine tissue (uncoated) was used as a control. *P<0.05 (vs. control), one-way analysis of variance (ANOVA) with post-hoc Bonferroni regression. Data are reported as mean ± SD across three replicates.

[0045] [Figure 33]Figure 33. Evaluation of glucose absorption recovery in animals treated with tissue-accelerated polymerization. Polydopamine nano-crosslinker (25 mg / ml) was first suspended in the tissue-accelerated polymerization solution. The tissue-accelerated polymerization solution (10 ml / kg) containing the nano-crosslinker was orally administered to pigs and introduced into the small intestine. The solution was administered directly into the small intestine through a catheter under endoscopic visual guidance. This was an ex vivo study. An oral glucose tolerance test was performed 24 hours after the pigs received the tissue-accelerated polymerization coating. The pigs with the tissue-accelerated polymerization coating regained normal glucose absorption after 24 hours, demonstrating that the tissue-accelerated polymerization coating layer was transient. Data were averaged across animals (each animal is represented by a gray line) in each group (indicated by a black line).

[0046] [Figure 34] Figure 34. Evaluation of CYP450 activity in epithelia with and without a polydopamine coating layer. Tissues (12 cm2) were exposed to tissue-accelerated polymerization solution (10 mL) for 20 minutes and washed three times with PBS buffer (1x) to remove excess polydopamine. This is an ex vivo study. No change in CYP3A4 activity was observed in epithelia after tissue-accelerated polymerization. Epithelial tissue (uncoated) was used as a control. The difference in activity was not statistically significant. P>0.05 by two-tailed t-test. Data are reported as the mean ± SD over five replicates.

[0047] [Figure 35]Figures 35A-35B. Tissue-accelerated polymerization patterns in the human GI tract. Tissue (12 cm2) was exposed to tissue-accelerated polymerization solution (10 mL) for 20 minutes and washed three times with PBS buffer (1x) to remove excess polydopamine. This is an ex vivo study. Figure 35A depicts images showing human tissue samples in different parts of the GI tract before and after applying the tissue-accelerated polymerization coating ex vivo. Samples (6 mm diameter) were collected at three random sites on the polydopamine-coated tissue. Figure 35B shows quantitative measurements of polydopamine signal intensity for the samples shown in Figure 35B. The difference in intensity between the small intestine and other tissues is statistically significant. *P<0.05, one-way analysis of variance (ANOVA) and post-hoc Bonferroni. Data are reported as the mean ± SD across three different tissue samples.

[0048] [Figure 36] Figures 36A-36C. Stability of surface-based polydopamine coatings. Polydopamine coatings were applied onto the surface of impermeable polycarbonate substrates. The polycarbonate sheets were exposed to a tissue-accelerating polymerization solution (without H2O2) for 36 hours and washed with water to remove excess polydopamine. The white polycarbonate (left) turned dark brown (right) after polydopamine coating. The stability of the polydopamine coating was evaluated under a range of physical conditions. Figures 36A-36C show that no obvious polydopamine signal reduction was observed under both gentle and vigorous scratching, and the polydopamine coating was only removed under extremely vigorous scratching with sandpaper. These results demonstrated that the stability of surface-based polydopamine is due to strong surface-based adhesion rather than penetration into the substrate surface.

[0049] [Figure 37]Figure 37. Conversion of the tissue-accelerated polymerization platform into capsules. Isolated porcine small intestine from in vivo showed different polydopamine coatings before and after clamping, demonstrating the coating performance of the tissue-accelerated polymerization capsules. The capsules consisted of dopamine hydrochloride powder (500 mg), Tris powder (30-300 mg; 300 mg), and solid urea HO powder (10-50 mg; 50 mg). The mixed powders were filled into (size 000) capsules. The capsules were administered directly into the intestine by surgically puncturing the intestine for capsule delivery. Due to the small intestinal ligation, the capsules did not descend to the lower small intestine. After releasing the tissue-accelerated polymerization components, the capsules disintegrated, dissolved, and broke into smaller pieces. DETAILED DESCRIPTION OF THE INVENTION

[0050] definition Unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular.

[0051] The phrases "in some embodiments" and "in an embodiment" are used interchangeably.

[0052] The following definitions are of more general terms used throughout this application:

[0053] The singular terms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. Similarly, the word "or" is intended to include "and" unless the context clearly dictates otherwise. Similarly, the word "or" is intended to include "and" unless the context clearly dictates otherwise.

[0054] Except in the examples, or where otherwise indicated, all numbers expressing quantities of ingredients or reaction conditions used herein should be understood to be modified in all instances by the term "about." "About" and "approximately" generally refer to an acceptable degree of error for the quantity measured given the nature or precision of the measurement. Exemplary degrees of error are within 20 percent (%), typically within 10%, or more typically within 5%, 4%, 3%, 2%, or 1% of a given value or range of values.

[0055] When a range of values ​​("range") is listed, it is intended to encompass each value and subrange within the range. Unless otherwise specified, the range includes the two endpoints of the range.

[0056] The terms "composition" and "formulation" are used interchangeably.

[0057] A "subject" to which administration is contemplated refers to a human (i.e., male or female of any age group, e.g., a pediatric subject (e.g., infant, child, adolescent) or an adult subject (e.g., young adult, middle-aged adult, or elderly adult)) or a non-human animal. In some embodiments, the non-human animal is a mammal (e.g., a primate (e.g., a cynomolgus monkey or a rhesus monkey), a commercially relevant mammal (e.g., a cow, pig, horse, sheep, goat, cat, or dog), or a bird (e.g., a commercially relevant bird, e.g., a chicken, duck, goose, or turkey)). In some embodiments, the non-human animal is a fish, reptile, or amphibian. The non-human animal can be male or female at any stage of development. The non-human animal can be a transgenic or genetically engineered animal. The term "patient" refers to a human subject in need of treatment for a disease.

[0058] The terms "administer," "administering," or "administration" refer to implanting, absorbing, ingesting, injecting, inhaling, or otherwise introducing a composition described herein into or onto a subject.

[0059] The terms "treatment," "treat," and "treating" refer to reversing, alleviating, delaying the onset of, or inhibiting the progression of a disease as described herein. In some embodiments, treatment may be administered after one or more signs or symptoms of a disease have occurred or are observed. In other embodiments, treatment may be administered in the absence of signs or symptoms of a disease. For example, treatment may be administered to a predisposed subject prior to the onset of symptoms (e.g., in light of a history of symptoms and / or exposure to a pathogen). Treatment may also be continued after symptoms have resolved, e.g., to delay or prevent recurrence.

[0060] The terms "condition," "disease," and "disorder" are used interchangeably.

[0061] An "effective amount" of a polymer or composition described herein refers to an amount sufficient to elicit a desired biological response. The effective amount of a polymer or composition described herein can vary depending on factors such as the desired biological endpoint, the pharmacokinetics of the polymer or composition, the condition to be treated, the mode of administration, and the age and health of the subject. In some embodiments, the effective amount is a therapeutically effective amount. In some embodiments, the effective amount is a prophylactic treatment. In some embodiments, the effective amount is the amount of a compound, polymer, or composition described herein in a single dose. In some embodiments, the effective amount is the combined amount of a compound, polymer, or composition described herein in a multiple dose.

[0062] The term "cancer" refers to a class of diseases characterized by the development of abnormal cells that have the ability to grow uncontrollably and invade and destroy normal body tissue. See, e.g., Stedman's Medical Dictionary, 25th ed.; Hensyl ed.; Williams & Wilkins: Philadelphia, 1990.

[0063] "Autoimmune disease" refers to a disease that results from an inappropriate immune response by a subject's body against substances and tissues that are normally present in the body. In other words, the immune system mistakes some part of the body for a pathogen and attacks its own cells. This can be restricted to one organ (e.g., autoimmune thyroiditis) or can involve specific tissues in different locations (e.g., Goodpasture's disease, which can affect the basement membrane of both the lungs and kidneys). Treatment of autoimmune diseases is typically by immunosuppression, e.g., medications that reduce the immune response.

[0064] The term "inflammatory disease" refers to a disease caused by, resulting from, or resulting in inflammation. The term "inflammatory disease" can also refer to a deregulated inflammatory response that causes an exaggerated response by macrophages, granulocytes, and / or T lymphocytes, leading to abnormal tissue damage and / or cell death. Inflammatory diseases can be either acute or chronic inflammatory conditions and can result from infectious or non-infectious causes.

[0065] The term "polymer" refers to a compound comprising 10 or more covalently joined repeating units. In some embodiments, the polymer is naturally occurring. In some embodiments, the polymer is synthetic (i.e., not naturally occurring).

[0066] The term "nanoparticle" refers to a particle having an average (e.g., mean) dimension (e.g., diameter) of between about 1 nanometer (nm) and about 1 micrometer (μm), inclusive (e.g., between about 1 nm and about 300 nm, between about 1 nm and about 100 nm, between about 1 nm and about 30 nm, between about 1 nm and about 10 nm, or between about 1 nm and about 3 nm).

[0067] As used herein, the term "drug" refers to a molecule, group of molecules, complex, or substance administered to an organism for diagnostic, therapeutic, preventative medical, or veterinary purposes. In some embodiments, the drug is an active pharmaceutical ingredient, a diagnostic agent, or a prophylactic agent. In some embodiments, the polymers and compositions disclosed herein include a drug(s), e.g., a first drug (e.g., at least one (e.g., at least two, including at least three). In some embodiments, the polymers and compositions can further include a second drug. In some embodiments, the drug is an enzyme (e.g., a digestive enzyme), a nutrient blocker (e.g., a crosslinking agent), a diagnostic agent, a dietary supplement, a radioprotectant, an active pharmaceutical ingredient, or a combination thereof.

[0068] As used herein, the term "radioprotectant" refers to an agent that protects biological systems exposed to radiation, either naturally or through radiation leakage. In some embodiments, radioprotectants protect normal cells from radiation damage in cancer patients undergoing radiation therapy.

[0069] As used herein, the term "diagnostic agent" refers to an imaging agent or contrast agent. The terms "imaging agent" and "contrast agent" refer to substances used in medical imaging to enhance the contrast of structures or fluids within the body. They are commonly used in medical imaging to enhance the visibility of blood vessels and the gastrointestinal tract.

[0070] As used herein, the term "active pharmaceutical ingredient" includes agents capable of providing local or systemic biological, physiological, or therapeutic effects in the biological system to which it is applied. For example, active pharmaceutical ingredients may act to control tumor growth, control infection or inflammation, act as an analgesic, promote anti-cell adhesion, and enhance bone growth, among other functions. Other suitable active pharmaceutical ingredients may include antivirals, hormones, antibodies, or therapeutic proteins. Other active pharmaceutical ingredients include prodrugs, which are drugs that are not biologically active when administered, but are converted by metabolism or some other mechanism to a biologically active agent upon administration to a subject.

[0071] The active pharmaceutical ingredient can be a chemical compound, such as an organic or inorganic small molecule; saccharin; oligosaccharides; polysaccharides; biological macromolecules, such as peptides, proteins, and peptide analogs and derivatives; peptidomimetics; antibodies and their antigen-binding fragments; nucleic acids; nucleic acid analogs and derivatives; extracts made from biological materials such as bacteria, plants, fungi, or animal cells; animal tissue; naturally occurring or synthetic compositions; and any combination thereof.

[0072] Examples of active pharmaceutical ingredients include, but are not limited to, antimicrobials, analgesics, anti-inflammatory drugs, counterirritants, coagulation regulators, diuretics, sympathomimetics, appetite suppressants, antacids, and other gastrointestinal drugs; antiparasitic drugs, antidepressants, antihypertensive drugs, anticholinergic drugs, stimulants, antihormonal drugs, central nervous system and respiratory stimulants, drug antagonists, lipid-regulating drugs, uricosurics, cardiac glycosides, electrolytes, ergot and its derivatives, expectorants, hypnotics and sedatives, antidiabetic drugs, dopaminergic drugs, antiemetics, muscle relaxants, parasympathomimetics, anticonvulsants, antihistamines, beta-blockers, laxatives, antiarrhythmic drugs, imaging materials, radiopharmaceuticals, antiallergic drugs, tranquilizers, vasodilators, antivirals, and other drugs with anti-neoplastic or cytostatic or anti-cancer properties, or combinations thereof.Other suitable active pharmaceutical ingredients include contraceptives and vitamins, as well as micronutrients and macronutrients. Still other examples include anti-infectives, such as antibiotics and antivirals; analgesics and analgesic combinations; appetite suppressants; anthelmintics; anti-arthritics; anti-asthmatics; anticonvulsants; antidepressants; antidiuretics; antidiarrheals; antihistamines; anti-inflammatory drugs; anti-migraine medications; anti-nausea medications; antineoplastics; anti-Parkinson's medications; antipruritics; antipsychotics; antipyretics, antispasmodics; anticholinergics; sympathomimetics; xanthine derivatives; cardiovascular medications, including calcium channel blockers and beta-blockers, such as pindolol and and antiarrhythmics; antihypertensives; diuretics; vasodilators, including general coronary, peripheral, and cerebral; central nervous system stimulants; cough and cold remedies, including decongestants; hormones, e.g., estradiol and other steroids, including corticosteroids; hypnotics; immunosuppressants; muscle relaxants; parasympatholytics; psychostimulants; sedatives; and tranquilizers; as well as naturally occurring or genetically engineered proteins, polysaccharides, glycoproteins, or lipoproteins.

[0073] DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS Before the disclosed systems, methods, uses, and kits are described in more detail, it is to be understood that the aspects described herein are not limited to specific embodiments, methods, systems, devices, or configurations, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular aspects only, and is not intended to be limiting, unless specifically defined herein.

[0074] In general, polydopamine polymerization is a slow process, and its reaction rate is limited by the low oxygen levels in typical dopamine oxidation conditions. 11 However, the inventors unexpectedly discovered that protective oxygen production by endogenous catalase degradation of hydrogen peroxide (known as the cellular antioxidant effect) boosts oxygen release for dopamine oxidation and dramatically increases the rate of dopamine polymerization. Figure 1A shows a schematic representation of one embodiment of the present disclosure in which an oral monomer solution containing dopamine monomers and hydrogen peroxide is administered to a subject. The solution flows unimpeded across the surface of the gastrointestinal mucosa, and hydrogen peroxide molecules diffuse freely between the epithelial tissue and the dopamine solution. However, dopamine remains in solution due to its slow diffusion and transport. Hydrogen peroxide diffuses into epithelial cells and is rapidly decomposed by intracellular catalase into oxygen, which is released outside the cells and mixes with the extracellular monomers. These monomers near the epithelial surface are rapidly oxidized to oligomers and further polymers, which crosslink with biomolecules exposed on the outside of the epithelium, forming a thin and strong polydopamine coating layer on the tissue. Unreacted monomers in solution and unbound polydopamine are washed away from the epithelium. This type of tissue surface-initiated polymeric coating avoids potential intestinal adhesions and obstruction frequently induced by bulk crosslinking-based sealants or other conventional tissue adhesives. 13Catalytic polydopamine polymerization occurs primarily in the small intestine due to higher catalase expression levels in the small intestine compared to other parts of the gastrointestinal tract, including the esophagus, stomach, and large intestine. Thus, specific small intestinal targeting is naturally possible due to the uneven distribution of the native enzyme along the digestive tract.

[0075] The inventors have conducted a series of investigations into the compositions, methods, and kits disclosed herein. Using a variety of techniques, including endoscopic examination, intestinal ligation, and X-ray imaging, the inventors have consistently demonstrated the robustness of the subject matter and demonstrated the characteristics of in situ formed polymers (prolonged but transient intestinal retention). Additionally, the inventors have not observed any clinical, endoscopic, or radiological evidence of gastrointestinal perforation, inflammation, or obstruction associated with the use of the compositions, methods, and kits disclosed herein. The biocompatibility of certain compositions disclosed herein has been carefully characterized by confirming their lack of oral toxicity in accordance with guidelines issued by the Organization for Economic Cooperation and Development (OECD).

[0076] Methods and Uses In one aspect, the disclosure provides a method for forming a polymer in situ in a subject, the method comprising administering to the subject a composition comprising a monomer and an oxygen source, wherein the monomer and oxygen source contact a catalyst endogenous to the subject, and the catalyst polymerizes the monomer, wherein the monomer is dopamine or a salt thereof, the oxygen source is hydrogen peroxide or urea hydrogen peroxide, and the endogenous catalyst is selected from catalase or peroxidase.

[0077] In some embodiments, the endogenous catalyst is a peroxidase, ie, eosinophil peroxidase, lactoperoxidase, or myeloperoxidase.

[0078] In some embodiments, the endogenous catalyst is catalase. In some embodiments, the catalase is bacterial catalase. In some embodiments, the catalase is human catalase.

[0079] In some embodiments, the endogenous catalyst is located in the gastrointestinal (GI) tract of the subject. In some embodiments, the endogenous catalyst is located in the upper GI of the subject. In some embodiments, the endogenous catalyst is located in the intestinal tract of the subject. In some embodiments, the endogenous catalyst is located in the stomach of the subject.

[0080] In some embodiments, the endogenous catalyst is located in a cell. In some embodiments, the endogenous catalyst is located in a blood cell. In some embodiments, the endogenous catalyst is located on a cell. In some embodiments, the endogenous catalyst is located on a blood cell. In some embodiments, the endogenous catalyst is located in a cell. In some embodiments, the endogenous catalyst is secreted by a blood cell. In some embodiments, the endogenous catalyst is located on a cell. In some embodiments, the endogenous catalyst is secreted by a blood cell.

[0081] In some embodiments, the composition further comprises an enzyme, a nutrient blocker, a radioprotectant, a dietary supplement, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof. In some embodiments, the composition further comprises an enzyme. In some embodiments, the composition further comprises a nutrient blocker. In some embodiments, the composition further comprises a radioprotectant. In some embodiments, the composition further comprises an active pharmaceutical ingredient. In some embodiments, the composition further comprises a diagnostic agent. In some embodiments, the composition further comprises a combination of two or more of a nutrient blocker, a radioprotectant, a dietary supplement, an active pharmaceutical ingredient, and a diagnostic agent.

[0082] In some embodiments, the oxygen source is hydrogen peroxide. In some embodiments, the oxygen source is hydrogen peroxide urea.

[0083] In some embodiments, at least one of the monomer and the oxygen source is stable in the stomach of the subject. In some embodiments, at least one of the monomer and the oxygen source is stable in the stomach of the subject for at least 30 minutes. In some embodiments, at least one of the monomer and the oxygen source is stable in the stomach of the subject for at least 60 minutes.

[0084] In some embodiments, the composition is stable in the subject's stomach. In some embodiments, the composition is stable in the subject's stomach for at least 30 minutes. In some embodiments, the composition is stable in the subject's stomach for at least 60 minutes.

[0085] In some embodiments, at least one of the monomer and the oxygen source is stable in that it is not decomposed in the stomach of a subject.In some embodiments, at least 95% of at least one of the monomer and the oxygen source remains after the composition has passed through the stomach of a subject.In some embodiments, at least 90% of at least one of the monomer and the oxygen source remains after the composition has passed through the stomach of a subject.In some embodiments, at least 80% of at least one of the monomer and the oxygen source remains after the composition has passed through the stomach of a subject.

[0086] In some embodiments, the composition comprises about 0.001 to about 1000 mg / mL of dopamine. In some embodiments, the composition comprises about 0.01 to about 100 mg / mL of dopamine. In some embodiments, the composition comprises about 0.01 to about 50 mg / mL of dopamine. In some embodiments, the composition comprises about 1 to about 20 mg / mL of dopamine. In some embodiments, the composition comprises 10 mg / mL of dopamine. In some embodiments, the composition comprises 9.8 mg / mL of dopamine.

[0087] In some embodiments, the composition comprises about 0.01 to about 100 mM of an oxygen source. In some embodiments, the composition comprises about 0.1 to about 50 mM of an oxygen source. In some embodiments, the composition comprises about 1 to about 30 mM of an oxygen source. In some embodiments, the composition comprises about 20 mM of an oxygen source.

[0088] In some embodiments, the composition comprises an oxygen source at a concentration compatible with ingestion by a subject.

[0089] In some embodiments, the composition further comprises a buffering agent.

[0090] In some embodiments, the composition has a pH of about 7 to about 10. In some embodiments, the composition has a pH of about 7 to about 9. In some embodiments, the composition has a pH of about 8.5. In some embodiments, the composition has a pH of about 7.4.

[0091] In some embodiments, the composition is administered by a route selected from oral, rectal, injection, sublingual, buccal, vaginal, ocular, otic, inhalation, or dermal. In some embodiments, the composition is administered orally. In some embodiments, the composition is administered by intra-articular injection. In some embodiments, the composition is administered topically. In some embodiments, the composition is administered dermatologically. In some embodiments, the composition is administered ophthalmically.

[0092] In some embodiments, the composition is administered via a scope, ie, an endoscope, arthroscope, cystoscope, colposcope, colonoscope, bronchoscope, ureteroscope, anoscope, esophagoscope, gastroscope, laparoscope, laryngoscope, neuroendoscope, rectoscope, sigmoidoscope, or thoracoscope.

[0093] In some embodiments, the composition is in a liquid or solid dosage form.

[0094] In some embodiments, the composition is in the form of a solution, gel, tablet, powder, capsule, eye drops, or transdermal patch. In some embodiments, the composition is in the form of a solution, gel, tablet, or capsule. In some embodiments, the composition is in the form of a solution. In some embodiments, the composition is in the form of eye drops. In some embodiments, the composition is in the form of a powder. In some embodiments, the composition is in the form of a transdermal patch.

[0095] In some embodiments, the polymer is formed in contact with and adheres to a tissue in a subject. In some embodiments, the polymer adheres to a tissue of the subject. In some embodiments, the tissue is epithelium. In some embodiments, the tissue is intestinal epithelium.

[0096] In some embodiments, the location of polymer formation is based on the expression level of the catalyst. In some embodiments, polymers are substantially formed in a particular tissue due to a high expression level of the catalyst. In some embodiments, polymers are substantially not formed in a particular tissue due to a low expression level of the catalyst. In some embodiments, the location of polymer formation is based on the expression level of catalase. In some embodiments, polymers are substantially formed in a particular tissue due to a low expression level of catalase. In some embodiments, polymers are substantially not formed in a particular tissue due to a low expression level of catalase.

[0097] In some embodiments, the tissue is epithelium. In some embodiments, the polymer is formed on and adheres to the epithelium of the subject. In some embodiments, the polymer is formed in contact with the epithelium of the subject. In some embodiments, the epithelium is intestinal epithelium. In some embodiments, the polymer is formed on the small intestine. In some embodiments, the polymer is formed in the lumen of the small intestine. In some embodiments, the polymer is formed on the epithelium of the duodenum of the subject.

[0098] In some embodiments, the polymer binds to amine moieties exposed on the intraluminal surface of the epithelium of the subject. In some embodiments, the polymer crosslinks to amine moieties exposed on the intraluminal surface of the epithelium of the subject.

[0099] In some embodiments, the polymer is formed rapidly. In some embodiments, the polymer is formed in less than about 20 minutes. In some embodiments, the polymer is formed in less than about 15 minutes. In some embodiments, the polymer is formed in less than about 12 minutes. In some embodiments, the polymer is formed in less than about 10 minutes. In some embodiments, the polymer is formed in less than about 5 minutes. In some embodiments, the polymer is formed in less than about 3 minutes. In some embodiments, the polymer is formed in less than about 2 minutes. In some embodiments, the polymer is formed in less than about 1 minute. In some embodiments, the polymer is formed almost instantly.

[0100] In some embodiments, the rate of polymerization is increased by at least 10-fold compared to polymer formation without endogenous catalase. In some embodiments, the rate of polymerization is increased by at least 50-fold compared to polymer formation without endogenous catalase. In some embodiments, the rate of polymerization is increased by at least 100-fold compared to polymer formation without endogenous catalase. In some embodiments, the rate of polymerization is increased by at least 150-fold compared to polymer formation without endogenous catalase. In some embodiments, the rate of polymerization is increased by at least 200-fold compared to polymer formation without endogenous catalase.

[0101] In some embodiments, the polymer is formed on the epithelium of the gastrointestinal tract of the subject. In some embodiments, the polymer is formed on the epithelium of the small intestine of the subject.

[0102] In some embodiments, the polymer does not form on the epithelium of the gastrointestinal tract outside the small intestine of the subject.

[0103] In some embodiments, the polymer is formed on the epithelium of one or more of the subject's duodenum, jejunum, ileum, colon, esophagus, or stomach. In some embodiments, the polymer is formed on the epithelium of the subject's duodenum. In some embodiments, the polymer is formed on the epithelium of the subject's jejunum. In some embodiments, the polymer is formed on the epithelium of the subject's ileum. In some embodiments, the polymer is formed on the epithelium of the subject's colon.

[0104] In some embodiments, the polymer is not substantially formed on the epithelium of one or more of the subject's esophagus or stomach. In some embodiments, the polymer is not substantially formed on the subject's esophagus and stomach. In some embodiments, the polymer is not formed on the subject's stomach and esophagus.

[0105] In some embodiments, the polymer forms less on the ileum and colon of the subject compared to the duodenum and jejunum of the subject.

[0106] In some embodiments, the polymer is formed on the villi of the epithelium of the subject.

[0107] In some embodiments, the polymer forms a temporary barrier in vivo. In some embodiments, the polymer forms a transient barrier in vivo.

[0108] In some embodiments, the polymer persists for about 30 minutes. In some embodiments, the polymer persists for about 1 hour. In some embodiments, the polymer persists for about 6 hours. In some embodiments, the polymer persists for about 12 hours. In some embodiments, the polymer persists for about 24 hours.

[0109] In some embodiments, about 20 to about 70% of the transient barrier remains after 12 hours. In some embodiments, about 30 to about 50% of the transient barrier remains after 12 hours. In some embodiments, about 20% of the transient barrier remains after 12 hours. In some embodiments, about 20 to about 70% of the transient barrier remains after 6 hours. In some embodiments, about 30 to about 50% of the transient barrier remains after 6 hours. In some embodiments, about 20% of the transient barrier remains after 6 hours. In some embodiments, about 20 to about 70% of the transient barrier remains after 3 hours. In some embodiments, about 30 to about 50% of the transient barrier remains after 3 hours. In some embodiments, about 20% of the transient barrier remains after 3 hours.

[0110] In some embodiments, the polymer is cleared from the subject after about 3 hours. In some embodiments, the polymer is cleared from the subject after about 6 hours. In some embodiments, the polymer is cleared from the subject after about 12 hours. In some embodiments, the polymer is cleared from the subject after about 24 hours. In some embodiments, the polymer is cleared from the subject after about 48 hours.

[0111] In some embodiments, the polymeric barrier allows selective molecular transport across the epithelium of the patient.

[0112] In some embodiments, the method is a method of modulating diffusion in a subject within the intestinal tract, hi some embodiments, the method modulates the diffusion of one or more of salts, ions, water, oxygen, carbon dioxide, carbonate anions, acids, bases, carbohydrates, lipids, proteins, nucleic acids, nutrients, or active pharmaceutical ingredients in a subject.

[0113] In some embodiments, the polymer modulates absorption of one or more nutrients or active pharmaceutical ingredients in the small intestine.

[0114] In some embodiments, the polymer substantially prevents absorption of one or more nutrients into the epithelium in which the polymer is formed, into the intestinal wall of the subject, or into the bloodstream of the subject.

[0115] In some embodiments, the method is a method of delivering a drug to a subject. In some embodiments, the drug is an active pharmaceutical ingredient. In some embodiments, the drug is an enzyme. In some embodiments, the drug is a radioprotector. In some embodiments, the drug is delivered to the intestinal tract.

[0116] In some embodiments, the method allows for sustained release of a drug in a subject. In some embodiments, the drug is an active pharmaceutical ingredient. In some embodiments, the drug is an enzyme. In some embodiments, the drug is a radioprotector. In some embodiments, the sustained release occurs within the GI tract.

[0117] In some embodiments, the method is a method of immobilizing an agent in a subject. In some embodiments, the agent is an active pharmaceutical ingredient. In some embodiments, the agent is an enzyme. In some embodiments, the agent is a radioprotector. In some embodiments, the agent is immobilized in the GI tract.

[0118] In some embodiments, the method is a method for localized delivery of a drug in a subject. In some embodiments, the drug is an active pharmaceutical ingredient. In some embodiments, the drug is an enzyme. In some embodiments, the drug is a radioprotector. In some embodiments, the localized delivery occurs within the GI tract.

[0119] In some embodiments, the method is a method of reducing the dosing frequency of a drug. In some embodiments, the drug is an active pharmaceutical ingredient. In some embodiments, the drug is an enzyme. In some embodiments, the drug is a radioprotectant.

[0120] In some embodiments, the method is a method of increasing the half-life of a drug in a subject. In some embodiments, the drug is an active pharmaceutical ingredient. In some embodiments, the drug is an enzyme. In some embodiments, the drug is a radioprotectant.

[0121] In some embodiments, the method is a method of increasing residence time of a drug in a subject. In some embodiments, the drug is an active pharmaceutical ingredient. In some embodiments, the drug is an enzyme. In some embodiments, the drug is a radioprotector. In some embodiments, the method is a method of increasing residence time of a drug in the GI tract.

[0122] In some embodiments, the method is a method of treating or preventing a disease in a subject. In some embodiments, the method is a method of treating a disease in a subject. In some embodiments, the method is a method of preventing a disease in a subject.

[0123] In some embodiments, the method is a method of assisting tissue repair and regeneration in a subject at the site of polymerization. In some embodiments, the method is a method of assisting tissue repair in a subject at the site of polymerization. In some embodiments, the method is a method of assisting tissue regeneration in a subject at the site of polymerization.

[0124] In some embodiments, the methods cause the subject's intestinal lumen to remain enlarged.

[0125] In some embodiments, the method is a method of preventing intestinal adhesions in a subject.

[0126] In some embodiments, the method is a method of preventing an intestinal obstruction in a subject.

[0127] In some embodiments, the method is a method of treating bleeding in a subject. In some embodiments, the bleeding is in the upper GI tract.

[0128] In some embodiments, the polymer and composition further comprise an enzyme. In some embodiments, the enzyme is a digestive enzyme. In some embodiments, the digestive enzyme is lactase, peptidase, sucrase, maltase, amylase, lipase, or protease. In some embodiments, the digestive enzyme is β-galactosidase.

[0129] In some embodiments, the method is a method of improving digestive efficiency by a subject.

[0130] In some embodiments, the method is a method of enhancing the digestion of sugar by a subject. In some embodiments, the method is a method of enhancing the digestion of lactose by a subject.

[0131] In some embodiments, the method is a method of treating lactose intolerance in a subject.

[0132] In some embodiments, the enzyme improves the subject's efficiency in digesting sugar by about 5-fold. In some embodiments, the enzyme improves the subject's efficiency in digesting sugar by about 10-fold. In some embodiments, the enzyme improves the subject's efficiency in digesting sugar by about 20-fold. In some embodiments, the enzyme improves the subject's efficiency in digesting lactose by about 40-fold. In some embodiments, the enzyme improves the subject's efficiency in digesting sugar by about 50-fold.

[0133] In some embodiments, β-galactosidase improves the subject's efficiency in digesting lactose by about 5-fold. In some embodiments, β-galactosidase improves the subject's efficiency in digesting lactose by about 10-fold. In some embodiments, β-galactosidase improves the subject's efficiency in digesting lactose by about 20-fold. In some embodiments, β-galactosidase improves the subject's efficiency in digesting lactose by about 40-fold. In some embodiments, β-galactosidase improves the subject's efficiency in digesting lactose by about 50-fold.

[0134] In some embodiments, the polymeric barrier does not inhibit the inherent digestive enzyme activity of the subject's epithelium.

[0135] In some embodiments, the polymer and composition further comprise a nutrient blocker.

[0136] In some embodiments, the method is a method of preventing nutrient absorption in a subject.

[0137] In some embodiments, the method is a method for regulating or controlling sugar absorption by a subject. In some embodiments, the sugar is selected from glucose, lactose, fructose, maltose, dextrose, galactose, sucrose, and isomaltose. In some embodiments, the method is a method for regulating or controlling glucose absorption by a subject.

[0138] In some embodiments, the method prevents absorption for less than about 48 hours. In some embodiments, the method prevents absorption for less than about 24 hours. In some embodiments, the method prevents absorption for less than about 12 hours. In some embodiments, the method prevents absorption for less than about 6 hours. In some embodiments, the method prevents absorption for less than about 3 hours.

[0139] In some embodiments, the method is a method of treating obesity in a subject.

[0140] In some embodiments, the method is a method of treating hyperinsulinemia in a subject.

[0141] In some embodiments, the method is a method of treating diabetes in a subject. In some embodiments, the diabetes is type 2 diabetes.

[0142] In some embodiments, the composition further comprises a cross-linking agent.

[0143] In some embodiments, the crosslinker comprises a nanoparticle. In some embodiments, the crosslinker comprises polydopamine. In some embodiments, the crosslinker is a nutrient blocking agent. In some embodiments, the crosslinker improves the nutrient blocking ability of the polymer.

[0144] In some embodiments, glucose absorption is controlled by adjusting the crosslink density of the polymer. In some embodiments, the method reduces glucose absorption by a subject by at least about 50%, at least about 60%, or at least about 70% for a 3-hour period following administration of the composition. In some embodiments, the method reduces glucose absorption by a subject by at least about 70% for a 3-hour period following administration of the composition. In some embodiments, the method reduces glucose absorption by a subject by at least about 50%, at least about 60%, or at least about 70% for a 2-hour period following administration of the composition. In some embodiments, the method reduces glucose absorption by a subject by at least about 50%, at least about 60%, or at least about 70% for a 1-hour period following administration of the composition.

[0145] In some embodiments, the method is a method of controlling or regulating nutrient uptake by a subject.

[0146] In some embodiments, the composition further comprises an active pharmaceutical ingredient. In some embodiments, the active pharmaceutical ingredient is an antiparasitic drug. In some embodiments, the active pharmaceutical ingredient is an anthelmintic drug. In some embodiments, the active pharmaceutical ingredient is praziquantel.

[0147] In some embodiments, the composition further comprises an active pharmaceutical ingredient. In some embodiments, the active pharmaceutical ingredient is an agent that treats an infectious disease. In some embodiments, the active pharmaceutical ingredient is an antiparasitic drug. In some embodiments, the active pharmaceutical ingredient is an anthelmintic drug. In some embodiments, the active pharmaceutical agent is an antiparasitic drug. In some embodiments, the active pharmaceutical ingredient is praziquantel. In some embodiments, the active pharmaceutical agent is an antiviral drug. In some embodiments, the active pharmaceutical agent treats influenza. In some embodiments, the active pharmaceutical agent treats type 2 diabetes. In some embodiments, the active pharmaceutical agent treats an ophthalmic disease. In some embodiments, the active pharmaceutical agent treats Crohn's disease. In some embodiments, the active pharmaceutical agent treats osteoarthritis. In some embodiments, the active pharmaceutical agent treats Alzheimer's disease.

[0148] In some embodiments, the active pharmaceutical ingredient treats a psychiatric disorder, Alzheimer's disease, an infectious disease, or transplant rejection. In some embodiments, the active pharmaceutical ingredient is a contraceptive, a statin, an antihypertensive, or an antibiotic.

[0149] In some embodiments, the active pharmaceutical ingredient is an anti-cancer agent. Anti-cancer agents include biotherapeutic anti-cancer agents and chemotherapeutic agents. Exemplary biotherapeutic anti-cancer agents include, but are not limited to, interferons, cytokines (e.g., tumor necrosis factor, interferon alpha, interferon gamma), vaccines, hematopoietic growth factors, monoclonal serum therapy, immunostimulatory and / or immunomodulatory agents (e.g., IL-1, 2, 4, 6, or 12), immune cell growth factors (e.g., GM-CSF), and antibodies (e.g., Herceptin (trastuzumab), T-DM1, Avastin (bevacizumab), Erbitux (cetuximab), Vectibix (panitumumab), Rituxan (rituximab), Bexar (tositumomab)). Exemplary chemotherapeutic agents include antiestrogens (e.g., tamoxifen, raloxifene, and megestrol), LHRH agonists (e.g., goserelin and leuprolide), antiandrogens (e.g., flutamide and bicalutamide), photodynamic therapy (e.g., verteporfin (BPD-MA), phthalocyanines, photosensitizer Pc4, and demethoxy-hypocrelin A (2BA-2-DMHA)), nitrogen mustards (e.g., cyclophosphamide, ifosfamide, trofosfamide, chlorambucil, estramustine, and melphalan), nitrosoureas (e.g., carmustine (BCNU) and lomustine (CCNU)), alkyl steroids (e.g., cyclophosphamide, ifosfamide, trofosfamide, chlorambucil, estramustine, and melphalan), and cyclosulfamethasone (cyclophosphamide, ifosfamide, trofosfamide, chlorambucil, estramustine, and melphalan). sulfonates (e.g., busulfan and treosulfan), triazenes (e.g., dacarbazine, temozolomide), platinum-containing compounds (e.g., cisplatin, carboplatin, oxaliplatin), vinca alkaloids (e.g., vincristine, vinblastine, vindesine, and vinorelbine), taxoids (e.g., paclitaxel or paclitaxel equivalents, such as nanoparticle albumin-bound paclitaxel (Abraxane), docosahexaenoic acid-bound paclitaxel (DHA-paclitaxel, Taxoplexin), polyglutamic acid-bound paclitaxel (PG-paclitaxel, paclitaxel poliglumex, CT-2103, Geotax),Tumor-activated prodrug (TAP) ANG1005 (Angiopep-2 conjugated to three molecules of paclitaxel), paclitaxel-EC-1 (paclitaxel conjugated to the erbB-recognizing peptide EC-1), and glucose-conjugated paclitaxel (e.g., 2'-paclitaxel methyl 2-glucopyranosyl succinate; docetaxel, taxol), epipodophyllin (e.g., etoposide, etoposide phosphate, teniposide, topotecan, 9-aminocamptothecin, camptoirinotecan, irinotecan, crisnatol, mitomycin C), antimetabolites agents, DHFR inhibitors (e.g., methotrexate, dichloromethotrexate, trimetrexate, edatrexate), IMP dehydrogenase inhibitors (e.g., mycophenolic acid, tiazofurin, ribavirin, and EICAR), ribonucleotide reductase inhibitors (e.g., hydroxyurea and deferoxamine), uracil analogs (e.g., 5-fluorouracil (5-FU), floxuridine, doxifluridine, raltitrexed, tegafur uracil, capecitabine), cytosine analogs (e.g., cytarabine C), cytosine arabinoside, and fludarabine), purine analogs (e.g., mercaptopurine and thioguanine), vitamin D3 analogs (e.g., EB1089, CB1093, and KH1060), isoprenylation inhibitors (e.g., lovastatin), dopaminergic neurotoxins (e.g., 1-methyl-4-phenylpyridinium ion), cell cycle inhibitors (e.g., staurosporine), actinomycins (e.g., actinomycin D, dactinomycin), bleomycins (e.g., bleomycin A2, bleomycin B2, peplomycin), anthracyclines (e.g., daunorubicin, doxorubicin, PEGylated liposomal doxorubicin, idarubicin, epirubicin, pirarubicin, zorubicin, mitoxantrone), MDR inhibitors (e.g., verapamil), Ca, 2+ATPase inhibitors (e.g., thapsigargin), imatinib, thalidomide, lenalidomide, tyrosine kinase inhibitors (e.g., axitinib (AG013736), bosutinib (SKI-606), cediranib (Resentin™, AZD2171), dasatinib (Sprycel®, BMS-354825), erlotinib (Tarceva®), gefitinib (Iressa®), imatinib (Gleevec®, CGP57148B, STI-571), lapatinib (Tykerb®, Tyverb ( Trademarks)), lestaurtinib (CEP-701), neratinib (HKI-272), nilotinib (Tasigna®), semaxanib (semaxinib, SET5416), sunitinib (Sutent®, SU11248), toceranib (Palladia®), vandetanib (Zactima®, ZD6474), vatalanib (PTK787, PTK / ZK), trastuzumab (Herceptin®), bevacizumab (Avastin®), rituximab (Rituxan®), cetuximab (Arbita®) (R), panitumumab (Vectibix (R), ranibizumab (Lucentis (R), nilotinib (Tasigna (R), sorafenib (Nexavar (R), everolimus (Afinitor (R), alemtuzumab (Campath (R), gemtuzumab ozogamicin (Mylotarg (R), temsirolimus (Torisel (R), ENMD-2076, PCI-32765, AC220, dovitinib lactate (TKI258, CHIR-258), BIBW2992 (T OVOK™), SGX523, PF-04217903, PF-02341066, PF-299804, BMS-777607, ABT-869, MP470, BIBF1120 (VARGATEF®), AP24534, JNJ-26483327, MGCD265, DCC-2036, BMS-690154, CEP-11981, tivozanib (AV-951), OSI-930, MM-121, XL-184, XL-647, and / or XL228), proteasome inhibitors (e.g., bortezomib (Velcade)),mTOR inhibitors (e.g., rapamycin, temsirolimus (CCI-779), everolimus (RAD-001), ridaforolimus, AP23573 (Ariad), AZD8055 (AstraZeneca), BEZ235 (Novartis), BGT226 (Norvartis), XL765 (Sanofi) Aventis), PF-4691502 (Pfizer), GDC0980 (Genetech), SF1126 (Semafoe), and OSI-027 (OSI), oblimersen, gemcitabine, carminomycin, leucovorin, pemetrexed, cyclophosphamide, dacarbazine, procarbazine, prednisolone, dexamethasone, campatecin, plicamycin, asparaginase, aminopterin, methopterin, porfiromycin, melphalan, leurocidin, leurosine, chlorambucil, trabectedin, procarbazine, discodermolide, carminomycin, aminopterin, and hexamethylmelamine.

[0150] In some embodiments, the active pharmaceutical ingredient is selected from the group including, but not limited to, antiproliferative drugs, anticancer drugs, angiogenesis inhibitors, anti-inflammatory drugs, immunosuppressants, antibacterial drugs, antiviral drugs, cardiovascular drugs, cholesterol-lowering drugs, antidiabetic drugs, antiallergic drugs, contraceptives, and painkillers. In some embodiments, the active pharmaceutical ingredient is an antiproliferative drug. In some embodiments, the active pharmaceutical ingredient is an anticancer drug. In some embodiments, the active pharmaceutical ingredient is an antiviral drug.

[0151] Exemplary active pharmaceutical ingredients include, but are not limited to, antibiotics, antivirals, anesthetics, anticoagulants, enzyme inhibitors, steroids, steroidal or nonsteroidal anti-inflammatory drugs, antihistamines, immunosuppressants, antigens, vaccines, antibodies, decongestants, sedatives, opioids, pain relievers, analgesics, antipyretics, hormones, and prostaglandins. Active pharmaceutical ingredients include small organic molecules such as drug compounds (e.g., compounds approved by the U.S. Food and Drug Administration as defined in the Code of Federal Regulations (CFR)), peptides, proteins, carbohydrates, monosaccharides, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides or proteins, small molecules linked to proteins, glycoproteins, steroids, nucleic acids, DNA, RNA, nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides, lipids, hormones, vitamins, and cells.

[0152] In some embodiments, the active pharmaceutical ingredient is an antibiotic. Exemplary antibiotics include penicillins (e.g., penicillin, amoxicillin), cephalosporins (e.g., cephalexin), macrolides (e.g., erythromycin, clarithromycin, azithromycin, troleandomycin), fluoroquinolones (e.g., ciprofloxacin, levofloxacin, ofloxacin), sulfonamides (e.g., cotrimoxazole, trimethoprim), tetracyclines (e.g., tetracycline, chlortetracycline, oxytetracycline, demeclocycline, methacycline, sancycline, doxycycline), and the like. Antibiotics include, but are not limited to, doxycline, aureomycin, terramycin, minocycline, 6-deoxytetracycline, lymecycline, meclocycline, methacycline, rolitetracycline, and glycylcycline antibiotics (e.g., tigecycline), aminoglycosides (e.g., gentamicin, tobramycin, paromomycin), aminocyclitols (e.g., spectinomycin), chloramphenicol, sparsomycin, quinupristin / dalfopristin (Syndercid™). In some embodiments, the antibiotic is a ribosome-targeting antibiotic.

[0153] In some embodiments, the active pharmaceutical ingredient is retained or encapsulated in the polymer. In some embodiments, the active pharmaceutical ingredient is retained or encapsulated on the polymer.

[0154] In some embodiments, the method is a method of increasing the residence time of the active pharmaceutical ingredient in a subject compared to administration of the active pharmaceutical ingredient in the absence of a polymer, hi some embodiments, the method is a method of increasing the residence time of the active pharmaceutical ingredient at the site of polymerization in a subject compared to administration of the active pharmaceutical ingredient in the absence of a polymer.

[0155] In some embodiments, the method is a method that allows for sustained release of the active pharmaceutical ingredient compared to administration of the active pharmaceutical ingredient in the absence of the polymer.

[0156] In some embodiments, the method reduces the dosing frequency of the active pharmaceutical ingredient compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the dosing frequency is once per day. In some embodiments, the dosing frequency is twice per day.

[0157] In some embodiments, the method increases the half-life of the active pharmaceutical ingredient compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the half-life of the active pharmaceutical ingredient by at least about two-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the half-life of the active pharmaceutical ingredient by at least about four-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the half-life of the active pharmaceutical ingredient by at least about six-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the half-life of the active pharmaceutical ingredient by at least about ten-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer.

[0158] In some embodiments, the method increases the AUC of the active pharmaceutical ingredient compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the AUC by at least about 2-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the AUC by at least about 3-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer. In some embodiments, the method increases the AUC by at least about 4-fold compared to administration of the active pharmaceutical ingredient in the absence of the polymer.

[0159] In some embodiments, the method reduces the C of the active pharmaceutical ingredient compared to administration of the active pharmaceutical ingredient in the absence of the polymer. max In some embodiments, the modulating method is an increase. In some embodiments, the modulating method is a decrease.

[0160] In some embodiments, the method reduces the T of the active pharmaceutical ingredient compared to administration of the active pharmaceutical ingredient in the absence of the polymer. max In some embodiments, the modulating method is an increase. In some embodiments, the modulating method is a decrease.

[0161] In some embodiments, the method does not affect drug metabolism once the active pharmaceutical ingredient is absorbed by the small intestine.

[0162] In some embodiments, the method is a method of treating schistosomiasis in a subject.

[0163] In some embodiments, the composition further comprises a radioprotector. In some embodiments, the radioprotector is an antioxidant, a thiol-containing compound, or a nitroxide. In some embodiments, the radioprotector is thalidomide, cysteine, amifostine, palifermin, or l-carnitine. In some embodiments, the radioprotector is thalidomide.

[0164] In some embodiments, the nutritional supplement is vitamin D or iron.

[0165] In some embodiments, the methods allow for targeting the small intestine.

[0166] In some embodiments, the method is a method for reducing uptake by the small intestine. In some embodiments, the method is a method for reducing uptake of one or more nutrients and active pharmaceutical ingredients by the small intestine. In some embodiments, the method is a method for reducing uptake of one or more nutrients by the small intestine. In some embodiments, the method is a method for reducing uptake of one or more active pharmaceutical ingredients by the small intestine.

[0167] In some embodiments, the method is a method for increasing the residence time in the small intestine. In some embodiments, the method is a method for increasing the residence time of one or more of nutrients and active pharmaceutical ingredients in the small intestine. In some embodiments, the method is a method for increasing the residence time of one or more of nutrients in the small intestine. In some embodiments, the method is a method for increasing the residence time of one or more of active pharmaceutical ingredients in the small intestine.

[0168] In some embodiments, the methods cause the intestinal lumen to remain enlarged.

[0169] In some embodiments, the method is a method of treating or preventing intestinal adhesions in a subject. In some embodiments, the method is a method of preventing intestinal adhesions in a subject.

[0170] In some embodiments, the method is a method of treating or preventing an ileus in a subject. In some embodiments, the method is a method of preventing an ileus in a subject.

[0171] In some embodiments, the method is a method of treating bleeding in a subject. In some embodiments, the method is a method of treating bleeding in the small intestine of a subject. In some embodiments, the bleeding is in the upper GI tract. In some embodiments, the bleeding is in the stomach. In some embodiments, the method of treating bleeding is a method of treating hemostasis.

[0172] In some embodiments, the polymer modulates absorption in the small intestine. In some embodiments, the polymer modulates absorption of one or more nutrients or active pharmaceutical ingredients, or a combination thereof, in the small intestine. In some embodiments, the polymer modulates absorption of one or more nutrients and active pharmaceutical ingredients in the small intestine. In some embodiments, the polymer modulates absorption of one or more nutrients in the small intestine. In some embodiments, the polymer modulates absorption of one or more active pharmaceutical ingredients in the small intestine.

[0173] In some embodiments, the polymer modulates digestion in the small intestine. In some embodiments, the polymer modulates digestion of one or more nutrients in the small intestine.

[0174] In some embodiments, the polymer substantially prevents absorption by the small intestine. In some embodiments, the polymer substantially prevents absorption of one or more nutrients or active pharmaceutical ingredients, or a combination thereof, into the epithelium on which the polymer is formed. In some embodiments, the polymer substantially prevents absorption of one or more nutrients into the epithelium on which the polymer is formed. In some embodiments, the polymer substantially prevents absorption of one or more active pharmaceutical ingredients into the epithelium on which the polymer is formed.

[0175] In some embodiments, the polymer substantially prevents absorption of one or more nutrients or active pharmaceutical ingredients, or a combination thereof, into the intestinal wall of the subject. In some embodiments, the polymer substantially prevents absorption of one or more nutrients into the intestinal wall of the subject. In some embodiments, the polymer substantially prevents absorption of one or more active pharmaceutical ingredients into the intestinal wall of the subject.

[0176] In some embodiments, the polymer substantially prevents absorption of one or more nutrients or active pharmaceutical ingredients, or a combination thereof, into the bloodstream of a subject. In some embodiments, the polymer substantially prevents absorption of one or more nutrients into the bloodstream of a subject. In some embodiments, the polymer substantially prevents absorption of one or more active pharmaceutical ingredients into the bloodstream of a subject.

[0177] In some embodiments, the method is a method of immobilizing an enzyme in a subject.

[0178] In some embodiments, the method is a method for delivering an active pharmaceutical ingredient to a subject.

[0179] In some embodiments, the method is a method of supplementing digestion in a subject.

[0180] In some embodiments, the polymer induces blood gelation. In some embodiments, the polymer induces clotting. In some embodiments, the composition is in the form of a powder. In some embodiments, the method includes spraying the powder onto an affected area in or on a subject.

[0181] In some embodiments, the polymer is non-toxic. In some embodiments, the composition is non-toxic. In some embodiments, the composition and its components are non-toxic.

[0182] In some embodiments, the polymer is stable to physical and chemical forces. In some embodiments, the polymer is stable to one or more of intestinal fluids, intestinal acids, gastric acid, chyme, ethanol, and saline. In some embodiments, the polymer degrades by less than about 25% when exposed to one or more of intestinal fluids, intestinal acids, gastric acid, chyme, ethanol, or saline. In some embodiments, the polymer degrades by less than about 20% when exposed to one or more of intestinal fluids, intestinal acids, gastric acid, chyme, ethanol, or saline. In some embodiments, the polymer degrades by less than about 10% when exposed to one or more of intestinal fluids, intestinal acids, gastric acid, chyme, ethanol, or saline. In some embodiments, the polymer degrades by less than about 5% when exposed to one or more of intestinal fluids, intestinal acids, gastric acid, chyme, ethanol, or saline. In some embodiments, the physical force is selected from one or more of peristalsis and articulation.

[0183] In another aspect, the present disclosure provides a method of treating a disease or disorder, comprising administering to a subject in need thereof an effective amount of a composition as described herein.

[0184] Further provided by the present disclosure is a method of preventing a disease or disorder, comprising administering to a subject in need thereof an effective amount of a composition as described herein.

[0185] In some embodiments, the disease or disorder is a metabolic disorder, an eye disease, a systemic disease, a digestive disorder, an infectious disease, cancer, bleeding, an ulcer, an intestinal obstruction, mesenteric ischemia, obesity, a psychiatric disorder, Alzheimer's disease, or transplant rejection. In some embodiments, the disease or disorder is a metabolic disorder, a systemic disease, a digestive disorder, an infectious disease, cancer, bleeding, an ulcer, an intestinal obstruction, mesenteric ischemia, obesity, a psychiatric disorder, Alzheimer's disease, or transplant rejection.

[0186] In some embodiments, the disease is cancer. Exemplary cancers include acoustic neuroma; adenocarcinoma; adrenal cancer; anal cancer; angiosarcoma (e.g., lymphangiosarcoma, lymphangioendothelial sarcoma, angiosarcoma); appendix cancer; benign monoclonal gammopathy; biliary tract cancer (e.g., biliary tract carcinoma); bladder cancer; breast cancer (e.g., adenocarcinoma of the breast, papillary carcinoma of the breast, adenocarcinoma of the breast, medullary carcinoma of the breast); brain tumors (e.g., meningioma, glioblastoma, glioma (e.g., astrocytoma, oligodendroglioma), medulloblastoma); bronchial cancer; carcinoid tumor; cervical cancer (e.g., cervical adenocarcinoma); choriocarcinoma; chordoma; craniopharyngioma; colorectal cancer (e.g., colon cancer, rectal cancer, colorectal adenocarcinoma); connective tissue cancer; epithelial carcinoma; ependymoma; endothelial sarcoma (Kaposi's sarcoma, multiple idiopathic hemorrhagic sarcoma); endometrial cancer (e.g., uterine carcinoma, uterine sarcoma); esophageal cancer (e.g., esophageal adenocarcinoma of the tract, Barrett's adenocarcinoma; Ewing's sarcoma; eye cancers (e.g., intraocular melanoma, retinoblastoma); familial eosinophilia; gallbladder cancer; stomach cancer (e.g., gastric adenocarcinoma); gastrointestinal stromal tumor (GIST); germ cell cancer; head and neck cancer (e.g., head and neck squamous cell carcinoma, oral cancer (e.g., oral squamous cell carcinoma), throat cancer (e.g., laryngeal cancer, pharyngeal cancer, nasopharyngeal cancer, oropharyngeal cancer); hematopoietic cancers (e.g., leukemias such as acute lymphocytic leukemia (ALL) (e.g., B-cell ALL, T-cell ALL), acute myeloid leukemia (AML) (e.g., B-cell AML, T-cell AML), chronic myeloid leukemia (CML) (e.g., B-cell CML, T-cell CML), and chronic lymphocytic leukemia (CLL) (e.g., B-cell CLL, T-cell CLL));Lymphomas, such as Hodgkin's lymphoma (HL) (e.g., B-cell HL, T-cell HL) and non-Hodgkin's lymphoma (NHL) (e.g., B-cell NHL, e.g., diffuse large cell lymphoma (DLCL) (e.g., diffuse large B-cell lymphoma), follicular lymphoma, chronic lymphocytic leukemia / small lymphocytic lymphoma (CLL / SLL), mantle cell lymphoma (MCL), marginal zone B-cell lymphoma (e.g., mucosa-associated lymphoid tissue (MALT) lymphoma, nodal marginal zone B-cell lymphoma, splenic marginal zone B-cell lymphoma), primary mediastinal B-cell lymphoma, Barton's lymphoma Kitt lymphoma, lymphoplasmacytic lymphoma (i.e., Waldenstrom's macroglobulinemia), hairy cell leukemia (HCL), immunoblastic large cell lymphoma, precursor B-lymphoblastic lymphoma, and primary central nervous system (CNS) lymphoma; and T-cell NHL, such as precursor T-lymphoblastic lymphoma / leukemia, peripheral T-cell lymphoma (PTCL) (e.g., cutaneous T-cell lymphoma (CTCL) (e.g., mycosis fungoides, Sézary syndrome), angioimmunoblastic T-cell lymphoma, extranodal natural killer T-cell lymphoma, enteropathic T-cell lymphoma , subcutaneous panniculitis-like T-cell lymphoma, and anaplastic large cell lymphoma; mixtures of one or more of the leukemias / lymphomas listed above; and multiple myeloma (MM)), heavy chain diseases (e.g., alpha chain diseases, gamma chain diseases, mu chain diseases); hemangioblastoma; hypopharyngeal carcinoma; inflammatory myofibroblastic tumor; immune cell amyloidosis; kidney cancer (e.g., nephroblastoma, also known as Wilms' tumor, renal cell carcinoma); liver cancer (e.g., hepatocellular carcinoma (HCC), malignant hepatoma); lung cancer (e.g., bronchogenic carcinoma, small cell lung cancer (SCLC), non-small cell lung cancer non-small cell lung cancer (NSCLC), lung adenocarcinoma; leiomyosarcoma (LMS); mastocytosis (e.g., systemic mastocytosis); muscle cancer; myelodysplastic syndromes (MDS); mesothelioma; myeloproliferative disorders (MPDs) (e.g., polycythemia vera (PV), essential thrombocythemia (ET), idiopathic myeloid metaplasia (AMM), also known as myelofibrosis (MF), chronic idiopathic myelofibrosis, chronic myelogenous leukemia (CML), chronic neutrophilic leukemia (CNL), hypereosinophilic syndrome (HES)); neuroblastoma; neurofibromas (e.g., neurofibromatosis (NF) type 1 or 2, schwannomatosis);Neuroendocrine cancers (e.g., gastrointestinal and pancreatic neuroendocrine tumors (GEP-NETs), carcinoid tumors); osteosarcomas (e.g., bone cancer); ovarian cancer (e.g., cystadenocarcinoma, ovarian embryonal carcinoma, ovarian adenocarcinoma); papillary adenocarcinoma; pancreatic cancer (e.g., andenocarcinoma, intraductal papillary mucinous neoplasm (IPMN), pancreatic islet cell tumor); penile cancer (e.g., Paget's disease of the penis and scrotum); pinealoma; primitive neuroectodermal tumor (PNT); plasma cell neoplasm; paraneoplastic syndromes; intraepithelial neoplasia; prostate cancer (e.g., prostate adenocarcinoma); rectal cancer; rhabdomyosarcoma; salivary gland cancer; skin cancer (e.g., These include, but are not limited to, squamous cell carcinoma (SCC), keratoacanthoma (KA), melanoma, and basal cell carcinoma (BCC); small intestine cancer (e.g., appendix cancer); soft tissue sarcomas (e.g., malignant fibrous histiocytoma (MFH), liposarcoma, malignant peripheral nerve sheath tumor (MPNST), chondrosarcoma, fibrosarcoma, and myxosarcoma); sebaceous gland carcinoma; small intestine cancer; sweat gland carcinoma; synovial tumor; testicular cancer (e.g., seminoma and testicular embryonal carcinoma); thyroid cancer (e.g., papillary thyroid carcinoma, papillary thyroid carcinoma (PTC), and medullary thyroid carcinoma); urethral cancer; vaginal cancer; and vulvar cancer (e.g., Paget's disease of the vulva).

[0187] In some embodiments, the metabolic disorder is hyperinsulinemia.

[0188] In some embodiments, the digestive system disease is Crohn's disease, ulcerative colitis, malabsorption, inflammatory bowel disease, irritable bowel syndrome, lactose intolerance, or celiac disease. In some embodiments, the disease is Crohn's disease.

[0189] In some embodiments, the disease is a psychiatric disorder.

[0190] In some embodiments, the disease is Alzheimer's disease.

[0191] In some embodiments, the disorder is transplant rejection.

[0192] In some embodiments, systemic disease is autoimmune disease.Exemplary autoimmune diseases include but are not limited to glomerulonephritis, Goodpasture's syndrome, necrotizing vasculitis, lymphadenitis, periarteritis nodosa, systemic lupus erythematosus, rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, psoriasis, ulcerative colitis, systemic sclerosis, dermatomyositis / polymyositis, antiphospholipid syndrome, scleroderma, pemphigus vulgaris, ANCA-associated vasculitis (for example, Wegener's granulomatosis, microscopic polyangiitis), uveitis, Sjogren's syndrome, Crohn's disease, Reiter's syndrome, ankylosing spondylitis, Lyme disease, Guillain-Barre syndrome, Hashimoto's thyroiditis and cardiomyopathy.

[0193] In some embodiments, the systemic disease is mastocytosis, chronic fatigue syndrome, systemic vasculitis, sarcoidosis, hypothyroidism, diabetes, fibromyalgia, adrenal insufficiency, celiac disease, ulcerative colitis, Crohn's disease, hypertension, metabolic syndrome, AIDS, Graves' disease, systemic lupus erythematosus, arthritis, atherosclerosis, sickle cell disease, myasthenia gravis, systemic sclerosis, an inflammatory disease, or sinusitis.

[0194] In some embodiments, the disease is an inflammatory disease. Inflammatory diseases include, but are not limited to, atherosclerosis, arteriosclerosis, autoimmune disorders, multiple sclerosis, systemic lupus erythematosus, polymyalgia rheumatica (PMR), gouty arthritis, osteoarthritis, tendonitis, bursitis, psoriasis, cystic fibrosis, osteoarthritis, rheumatoid arthritis, inflammatory arthritis, Sjogren's syndrome, giant cell arteritis, progressive systemic sclerosis (scleroderma), ankylosing spondylitis, polymyositis, dermatomyositis, pemphigus, pemphigoid, diabetes (e.g., type 1), myasthenia gravis, Hashimoto's thyroiditis, Graves' disease, Good's disease, and the like. Pasture's disease, mixed connective tissue disease, sclerosing cholangitis, inflammatory bowel disease, Crohn's disease, ulcerative colitis, pernicious anemia, inflammatory dermatoses, usual interstitial pneumonitis (UIP), asbestosis, silicosis, bronchiectasis, beryllium pneumonia, talc pneumonia, pneumoconiosis, sarcoidosis, desquamative interstitial pneumonia, lymphocytic interstitial pneumonia, giant cell interstitial pneumonia, cellular interstitial pneumonia, extrinsic allergic alveolitis, Wegener's granulomatosis and related forms of vasculitis (temporal arteritis and polyarteritis nodosa), inflammatory dermatoses, hepatitis, delayed hypersensitivity reactions ( Examples include poison ivy dermatitis, pneumonia, airway inflammation, adult respiratory distress syndrome (ARDS), encephalitis, immediate hypersensitivity reactions, asthma, hay fever, allergies, acute anaphylaxis, rheumatic fever, glomerulonephritis, pyelonephritis, cellulitis, cystitis, chronic cholecystitis, ischemia (ischemic injury), reperfusion injury, allograft rejection, host-versus-graft rejection, appendicitis, arteritis, blepharitis, bronchiolitis, bronchitis, cervicitis, cholangitis, chorioamnionitis, conjunctivitis, dacryoadenitis, dermatomyositis, endocarditis, endometritis, enteritis, small intestine colitis, and epicondylitis. Inflammatory diseases of the eye include, but are not limited to, epididymitis, fasciitis, fibrositis, gastritis, gastroenteritis, gingivitis, ileitis, iritis, laryngitis, myelitis, myocarditis, nephritis, omphalitis, oophoritis, orchitis, osteitis, otitis, pancreatitis, parotitis, pericarditis, pharyngitis, pleuritis, phlebitis, pneumonitis, proctitis, prostatitis, rhinitis, salpingitis, sinusitis, stomatitis, synovitis, orchitis, tonsillitis, urethritis, cystitis, uveitis, vaginitis, vasculitis, vulvitis, vulvovaginitis, vasculitis, chronic bronchitis, osteomyelitis, optic neuritis, temporal arteritis, transverse myelitis, necrotizing fasciitis, and necrotizing enterocolitis. Inflammatory diseases of the eye include, but are not limited to, postoperative inflammation. In some embodiments, the disease is an inflammatory joint disease. In some embodiments, the disease is arthritis. In some embodiments, the disease is osteoarthritis.In some embodiments, the disease is rheumatoid arthritis.

[0195] In some embodiments, the disease is obesity, hyperinsulinemia, or diabetes. In some embodiments, the disease is obesity. In some embodiments, the disease is hyperinsulinemia. In some embodiments, the disease is diabetes. In some embodiments, the disease is type 2 diabetes.

[0196] In some embodiments, the disease is an infectious disease. In some embodiments, the disease is a bacterial, viral, fungal, or parasitic infection. In some embodiments, the disease is a parasitic disease. In some embodiments, the disease is giardiasis, ascariasis, or tapeworm infection. In some embodiments, the disease is schistosomiasis. In some embodiments, the disease is a viral infection. In some embodiments, the disease is influenza.

[0197] In some embodiments, the disease is lactose intolerance.

[0198] In some embodiments, the disorder is intestinal obstruction. In some embodiments, the disorder is intestinal adhesions. In certain embodiments, the methods and compositions described herein prevent intestinal re-adhesion and / or re-obstruction.

[0199] In some embodiments, the methods and compositions provided herein are contraceptives.

[0200] In some embodiments, the condition is trauma.

[0201] In some embodiments, the present disclosure enables use of the composition to form a polymer in vivo, comprising administering to a subject a composition comprising a monomer and an oxygen source, wherein the monomer and oxygen source contact a catalyst endogenous to the subject in vivo, and the catalyst polymerizes the monomer in situ, wherein the monomer is dopamine or a salt thereof.

[0202] In another aspect, the disclosure enables use of the composition to form a polymer in vivo, comprising administering to a subject a composition comprising a monomer and an oxygen source, wherein the monomer and oxygen source contact a catalyst endogenous to the subject in vivo, and the catalyst polymerizes the monomer in situ, wherein the monomer is dopamine or a salt thereof, the oxygen source is hydrogen peroxide or hydrogen peroxide urea, and the endogenous catalyst is selected from catalase or peroxidase.

[0203] In one aspect, the present disclosure enables the use of an effective amount of a composition as described herein to treat a disease or disorder in a subject in need thereof.

[0204] In a further aspect, the present disclosure enables the use of an effective amount of a composition as described herein to prevent a disease or disorder in a subject in need thereof.

[0205] composition In one aspect, further provided herein is a composition comprising dopamine, an oxygen source, and optionally a buffering agent.

[0206] In some embodiments, the composition comprises about 0.001 to about 1000 mg / mL dopamine, about 0.01 to about 100 mM oxygen source, and optionally a buffering agent.

[0207] In some embodiments, the composition comprises about 0.001 to about 1000 mg / mL of dopamine. In some embodiments, the composition comprises about 0.001 to about 500 mg / mL of dopamine. In some embodiments, the composition comprises about 0.01 to about 100 mg / mL of dopamine. In some embodiments, the composition comprises about 1 to about 20 mg / mL of dopamine. In some embodiments, the composition comprises about 10 mg / mL of dopamine. In some embodiments, the composition comprises about 9.8 mg / mL of dopamine.

[0208] In some embodiments, the composition comprises about 0.01 to about 100 mM of the oxygen source. In some embodiments, the composition comprises about 0.1 to about 50 mM of the oxygen source. In some embodiments, the composition comprises about 1 to about 30 mM of the oxygen source. In some embodiments, the composition comprises about 20 mM of the oxygen source. In some embodiments, the composition comprises a concentration of the oxygen source compatible with ingestion by a subject.

[0209] In some embodiments, the composition has a pH of about 7 to about 10. In some embodiments, the composition has a pH of about 7 to about 9. In some embodiments, the composition has a pH of about 8.5. In some embodiments, the composition has a pH of about 7.4.

[0210] In some embodiments, the composition comprises about 10 mg / mL dopamine, about 20 mM hydrogen peroxide or urea hydrogen peroxide, and optionally a buffer. In some embodiments, the composition comprises about 9.8 mg / mL dopamine, about 20 mM hydrogen peroxide or urea hydrogen peroxide, and optionally a buffer.

[0211] In some embodiments, the buffer comprises phosphoric acid, acetic acid, citric acid, N-[tris(hydroxymethyl)methyl]glycine), (tris(hydroxymethyl)aminomethane), or (2-(bis(2-hydroxyethyl)amino)acetic acid). In some embodiments, the buffer comprises tris(hydroxymethyl)aminomethane.

[0212] In some embodiments, the composition further comprises a digestive enzyme, a nutrient blocker, a radioprotectant, a dietary supplement, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof.

[0213] In some embodiments, the composition is in a liquid or solid dosage form, hi some embodiments, the composition is in the form of a solution, gel, tablet, or capsule.

[0214] kit Also encompassed by the present disclosure are kits (e.g., pharmaceutical packs). The provided kits can include a composition described herein and a container (e.g., a vial, an ampoule, a bottle, a syringe, and / or a dispenser package, or other suitable container).

[0215] The present disclosure also provides kits. In one aspect, the present disclosure provides a kit comprising: a composition as described herein and instructions for administering the composition. In some embodiments, the composition comprises: dopamine; hydrogen peroxide or urea hydrogen peroxide; a buffer; and optionally, an enzyme, a nutrient blocker, a radioprotectant, a dietary supplement, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof. In some embodiments, the buffer is tris(hydroxymethyl)aminomethane. In some embodiments, the kit further comprises an endoscope, an arthroscope, a cystoscope, a colposcope, a colonoscope, a bronchoscope, a ureteroscope, an anoscope, an esophagoscope, a gastroscope, a laparoscope, a laryngoscope, a neuroendoscope, a rectoscope, a sigmoidoscope, or a thoracoscope. In some embodiments, the composition is in the form of a capsule.

[0216] In some embodiments, the provided kit may optionally further include a second container containing a pharmaceutical excipient for diluting or suspending the composition described herein. In some embodiments, the compositions provided by the first container and the second container are combined to form a single unit dosage form.

[0217] Thus, in one aspect, provided is a kit comprising a first container containing the composition described herein.In some embodiments, the kit is useful for treating a disease in a subject in need thereof.In some embodiments, the kit is useful for preventing a disease in a subject in need thereof.In some embodiments, the kit is useful for reducing the risk of developing a disease in a subject in need thereof.

[0218] In some embodiments, the kits described herein further include instructions for using the kit. The kits described herein may also include information required by regulatory authorities, such as the US Food and Drug Administration (FDA). In some embodiments, the information included in the kit is prescribing information. In some embodiments, the kit and instructions enable a subject in need thereof to treat a disease. In some embodiments, the kit and instructions enable a subject in need thereof to prevent a disease (in a subject in need thereof). In some embodiments, the kit and instructions enable a subject in need thereof to reduce the risk of developing a disease. The kits described herein may include one or more additional agents described herein as separate compositions.

[0219] Administration The methods and uses described herein involve administering to a subject an effective amount of a composition comprising a monomer and an oxygen source (i.e., to form a polymer in situ (e.g., to treat or prevent a disease)).

[0220] In some embodiments, the composition is administered orally. In some embodiments, the composition is in a liquid or solid dosage form. In some embodiments, the composition is in the form of a solution, gel, tablet, or capsule.

[0221] In some embodiments, the effective amount is a therapeutically effective amount. In some embodiments, the effective amount is an amount effective to treat an infectious disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent an infectious disease in a subject in need thereof. In some embodiments, the effective amount is a prophylactically effective amount. In some embodiments, the effective amount is an amount effective to treat a proliferative disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent a proliferative disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to treat a hematological disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent a hematological disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to treat a neurological disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent a neurological disease in a subject in need thereof. In some embodiments, the effective amount is an amount effective to treat a painful condition in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent a painful condition in a subject in need thereof. In some embodiments, the effective amount is an amount effective to treat a psychiatric disorder in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent a psychiatric disorder in a subject in need thereof. In some embodiments, the effective amount is an amount effective to treat a metabolic disorder in a subject in need thereof. In some embodiments, the effective amount is an amount effective to prevent a metabolic disorder in a subject in need thereof. In some embodiments, the effective amount is an amount effective to reduce the risk of developing a disease (e.g., an infectious disease, a proliferative disease, a blood disorder, a neurological disorder, a painful condition, a psychiatric disorder, or a metabolic disorder) in a subject in need thereof. In some embodiments, the effective amount is an amount effective to inhibit biological activity (e.g., aberrant activity such as increased activity) in a subject or cell.

[0222] In some embodiments, the subject is an animal. The animal can be of either sex and at any stage of development. In some embodiments, the subject described herein is a human. In some embodiments, the subject is an adult human. In some embodiments, the subject is a child. In some embodiments, the subject is a non-human animal. In some embodiments, the subject is a mammal. In some embodiments, the subject is a non-human mammal. In some embodiments, the subject is a domestic animal, such as a dog, cat, dairy cow, pig, horse, sheep, or goat. In some embodiments, the subject is a companion animal, such as a dog or cat. In some embodiments, the subject is a livestock animal, such as a dairy cow, pig, horse, sheep, or goat. In some embodiments, the subject is an exhibition animal. In other embodiments, the subject is a research animal, such as a rodent (e.g., mouse, rat), dog, pig, or non-human primate. In some embodiments, the animal is a genetically engineered animal. In some embodiments, the animal is a transgenic animal (e.g., transgenic mouse and transgenic pig). In some embodiments, the subject is a fish or reptile.

[0223] The compositions may be prepared, packaged, and / or sold in bulk, as a single unit dose, and / or as a plurality of single unit doses. A "unit dose" is a discrete amount of a composition comprising a predetermined amount of a drug or active ingredient. The amount of a drug or active ingredient is generally equal to the dosage of the drug or active ingredient that would be administered to a subject, and / or a convenient fraction of such a dosage, for example, one-half or one-third of such a dosage.

[0224] While the description of compositions provided herein is primarily directed to compositions suitable for administration to humans, it will be understood by those skilled in the art that such compositions are generally suitable for administration to animals of all kinds. Modifications of compositions suitable for administration to humans to make them suitable for administration to a variety of animals are well understood, and a veterinary pharmacologist of ordinary skill can design and / or make such modifications by routine experimentation.

[0225] The compositions provided herein are typically formulated in dosage unit form for ease of administration and uniformity of dosage. However, it will be understood that the total daily usage amount of the compositions described herein will be determined by a physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular subject or organism will depend on various factors, including the severity of the disease and disorder to be treated; the activity of the specific drug or active ingredient used; the specific composition used; the subject's age, weight, general health, sex, and diet; the administration time, administration route, and excretion rate of the specific drug or active ingredient used; the duration of treatment; drugs used in combination or simultaneously with the specific drug or active ingredient used; and similar factors well known in the medical field.

[0226] The compositions provided herein can be administered by any route, including enteral (e.g., oral), parenteral, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, subcutaneous, intraventricular, transdermal, intradermal, rectal, ophthalmic, intravaginal, intraperitoneal, topical, mucosal, nasal, buccal, sublingual; intratracheal instillation, bronchial instillation, and / or inhalation; and / or oral spray, nasal spray, and / or aerosol. Also contemplated is a direct administration to the affected area. Generally, the most appropriate administration route will depend on various factors, including the nature of the drug (e.g., its stability in the gastrointestinal environment) and / or the condition of the subject (e.g., whether the subject can tolerate oral administration). In some embodiments, the administration route is local (to the skin, eye, ear, mouth, or affected area).

[0227] The exact amount of agent(s) or agent(s) or active ingredient required to achieve an effective dose will vary between subjects, depending, for example, on the subject's species, age, and general condition, the severity of side effects or disorders, the identity of the specific agent(s) or active ingredient(s), the mode of administration, and the like. An effective amount can be contained in a single dose (e.g., an oral single dose) or multiple doses (e.g., oral multiple doses). In some embodiments, when multiple doses are administered to a subject or applied to tissues or cells, any two doses of the multiple doses contain different or substantially the same amounts of the agent(s) or active ingredient(s) described herein. In some embodiments, when multiple doses are administered to a subject or applied to tissues or cells, the frequency with which the multiple doses are administered to the subject or applied to tissues or cells is 3 doses per day, 2 doses per day, 1 dose per day, 1 dose every 2 days, 1 dose every 3 days, 1 dose per week, 1 dose per 2 weeks, 1 dose per 3 weeks, or 1 dose per 4 weeks. In some embodiments, the frequency of administering multiple doses to a subject or applying multiple doses to a tissue or cell is 1 dose per day. In some embodiments, the frequency of administering multiple doses to a subject or applying multiple doses to a tissue or cell is 2 doses per day. In some embodiments, the frequency of administering multiple doses to a subject or applying multiple doses to a tissue or cell is 3 doses per day. In some embodiments, when administering multiple doses to a subject or applying multiple doses to a tissue or cell, the length of time between the first and last dose of the multi-doses is 1 day, 2 days, 4 days, 1 week, 2 weeks, 3 weeks, 1 month, 2 months, 3 months, 4 months, 6 months, 9 months, 1 year, 2 years, 3 years, 4 years, 5 years, 7 years, 10 years, 15 years, 20 years, or the lifetime of the subject, tissue, or cell. In some embodiments, the length of time between the first and last dose of the multi-doses is 3 months, 6 months, or 1 year. In some embodiments, the length of time between the first and last dose of a multi-dos regimen is the lifetime of the subject, tissue, or cell.

[0228] The compositions as described herein can be administered in combination with one or more additional pharmaceutical agents (e.g., therapeutically and / or prophylactically active agents).The compositions can be administered in combination with additional pharmaceutical agents that improve their activity (e.g., activity (e.g., potency and / or efficacy) in treating a disease in a subject in need thereof, preventing a disease in a subject in need thereof, reducing the risk of developing a disease in a subject in need thereof, and / or inhibiting the activity of an organism in a subject or cell), improve bioavailability, improve safety, reduce drug resistance, reduce and / or alter metabolism, inhibit excretion, and / or alter distribution in a subject or cell.It will also be understood that the treatments used can achieve the desired effect for the same disorder, and / or they can achieve different effects.

[0229] The compositions can be administered simultaneously with, prior to, or subsequent to one or more additional pharmaceutical agents, which can be useful, for example, as a combination therapy. [Example]

[0230] example In order that the invention described herein may be more fully understood, the following examples are set forth. The examples described in this application are provided to illustrate the compounds, compositions, and methods provided herein and should not be construed in any way as limiting the scope thereof.

[0231] Abbreviation PDA: Polydopamine

[0232] CAT: Catalase

[0233] GSEL: synthetic epithelial lining of the gastrointestinal tract

[0234] PBS: phosphate buffered saline

[0235] TBS: Tris-buffered saline

[0236] TBST: Tris-buffered saline containing Triton X 100

[0237] IgG: immunoglobulin G

[0238] mRNA: messenger ribonucleic acid

[0239] cDNA complementary deoxyribonucleic acid

[0240] FTIR: Fourier transform infrared

[0241] Chemicals and Materials Dopamine hydrochloride (1225204), simulated gastric fluid (18818), urea HO (289132), catalase (C3556 & C1345), Triton X-100 (T8787), 3-amino-1,2,4-triazole (A8056), RIPA buffer (R0278), protease inhibitor cocktail (P8340), phosphatase inhibitor cocktail 3 (P0044), Trizma base, o-nitrophenol-β-D-galactoside (N1127), β-galactosidase (1356698), glucose (G8270), and lactose (17814) were purchased from Sigma-Aldrich. Formalin (10%, phosphate buffered) (SF100), Tissue-Plus OCT (23-730-571), BD GasPak EZ gas generating system incubation vessel (B260002), and SouthernBiotech Fluoromount-G slide mounting system (OB100) were purchased from Fisher Scientific. Pierce BCA Protein Assay (23225), Pierce DAB Substrate (34002), Pierce 16% Formaldehyde (w / v) (28908), Rabbit Anti-Goat Immunoglobulin G (IgG) (H+L) Secondary Antibody-HRP (31402), Goat Anti-Rabbit IgG (H+L) Secondary Antibody-HRP (65-6120), Vybrant MTT Cell Viability Assay (V13154), Dynabeads M-280 (Tosyl-Activated) (14203), SeeBlue Plus2 Prestained Protein Standard (LC5925), SuperSignal West Femto Maximum Sensitivity Substrate (34094), Pierce ECL Western Blotting Substrate (32109), NuPAGE 4-12% Bis-Tris protein gel (NP0321BOX), NE-PER Nuclear and Cytoplasmic Extraction Kit (78835), Mem-PER plus Membrane Protein Extraction Kit (89842), Antibiotic-Antimycotic (100×) (15240062), SuperScript IV Reverse Transcriptase (18090050), PCR Master Mix, and all primers were purchased from ThermoFisher.A total RNA isolation kit was purchased from ZYMO RESEARCH. Precision Plus Protein Dual Color Standard (#1610374) was purchased from Bio-Rad. Barium sulfate (13989) was purchased from Alfa Aesar. Simulated intestinal fluid was purchased from VWR. CYP3A4 activity assay kit (ab211076), calcium assay kit (ab102505), glutamate assay kit (ab138883), anti-catalase, and anti-β-actin antibodies were purchased from Abcam. A catalase (CAT) assay kit (E-BC-K031) was purchased from Elabscience. Praziquantel was purchased from Ark Pharm. Sieves (150 and 300 μm mesh size) were purchased from McMaster-Carr. A Glo-Tip spray catheter (G24892) was purchased from COOK Medical. All other chemicals and biochemicals (unless specified) were purchased from Sigma-Aldrich and used without further purification.

[0242] method. General. In vivo tissue-accelerated polymerization coating performance was evaluated in the GI tract of a large animal (porcine) model through multiple techniques, including endoscopy, intestinal ligation, and X-ray imaging. Yorkshire pigs (45-55 kg) were chosen as a model given their anatomical and genomic similarities to the human digestive system. Biocompatibility was characterized according to OECD guidelines. All animal experiments were approved by and conducted in accordance with the Committee on Animal Care at Massachusetts Institute of Technology. 19,28 Group and sample sizes for each experiment are indicated in the legends of each figure. Independent experiments for each sample were performed on different animals. All rats were randomly assigned to different experimental groups, however, there was no pre-established randomization plan for the in vivo pig study, as pigs were only available on demand.

[0243] Preparation of tissue-accelerated polymerization solution. Dopamine hydrochloride powder (500 mg) was rapidly dissolved in Tris buffer (50 mM, 50 ml) at pH 8.5, followed by the rapid addition of H2O2 (1 M, 1 ml). The mixed Tris-buffered tissue-accelerated polymerization solution was used fresh. The mixed solution was used fresh throughout all experiments unless otherwise noted, and is referred to as the tissue-accelerated polymerization solution and the gastrointestinal synthetic epithelial lining (GSEL). To prepare tissue-accelerated polymerization capsules, solid urea H2O2 (as a substitute for H2O2 solution) was used. Capsules were prepared using dopamine hydrochloride powder (500 mg), Tris powder (30–300 mg; e.g., 300 mg), and solid urea H2O2 powder (10–50 mg; e.g., 50 mg). The mixed powders were filled into (size 000) capsules. (See Figure 37.)

[0244] In vitro evaluation of catalase-catalyzed polydopamine polymerization. A tissue-accelerating polymerization solution (200 μl) was first prepared and added to a 96-well plate, followed by the addition of catalase (1 mg / mL, 5 μl in 1× phosphate-buffered saline (PBS) buffer). All solutions were placed in a low-oxygen chamber (BD GasPak EZ). The reaction solutions were kept at 37°C for 10–130 min. The attenuation of the solutions at 700 nm was measured using an Infinite M200 plate reader (Tecan). Results were compared with those obtained from solutions without catalase and HO and from reactions under conventional conditions (in air). See Figures 1C–1D and 6A–6B.

[0245] Characterization of polydopamine using FTIR and UV-Vis spectroscopy. Polydopamine standards were prepared by polymerization of dopamine solutions (10 mg / mL) under conventional conditions (in air) for 24 hours. (H. Lee, SM Dellatore, WM Miller, PB Messersmith, Mussel-inspired surface chemistry for multifunctional coatings. Science 318, 426-30 (2007)). For catalase-catalyzed polydopamine, polydopamine, catalase (1 mg / mL) were placed in a dialysis device (5 ml) and merged into tissue-accelerated polymerization solution (100 ml) for 24 hours. After the reaction, the polydopamine solution was measured by FTIR (Nicolet) and UV-Vis spectroscopy (Varian Cary 100). See Figures 7A-7D and 8A-8C.

[0246] Ex vivo evaluation of tissue-accelerated polymerization coating performance. Pig tissue was obtained from Blood Farm Slaughterhouse (West Groton, USA). Pigs were euthanized, and fresh tissue was excised and stored on ice. Human tissue specimens were obtained from four donors of different ages, races, and genders (National Disease Research Interchange, NDRI, USA). Tissue (12 cm) was placed in tissue-accelerated polymerization solution (10 mL). 2) and washed three times with PBS buffer (1x) to remove excess polydopamine. Samples (6 mm diameter) were collected at three to five random sites on the polydopamine-coated tissue, and images of the samples were analyzed for quantification of polydopamine coating. ImageJ was used to identify regions of interest that encompassed the polydopamine-coated tissue and excluded areas without tissue (blank). The same analysis was performed on all samples in each group to obtain the overall average polydopamine signal intensity and analyze signal variability. To remove bacteria in the mucus, the tissue was exposed to an antibiotic-antimycotic solution (10x, Gibco) and washed three times with PBS buffer (1x). Villi were stripped from the luminal surface (longitudinal opening) of small intestinal tissue placed on an ice-cooled substrate. Cell fractionation was performed on the villi using a cytoplasmic and membrane extraction kit (NE-PER, Mem-PER) according to the kit's protocol. CYP3A4 activity was measured by CYP3A4 activity assay (Abcam) based on the assay protocol. See Figures 1E–1H, 5A–5G, 9, 13, 15, 24, 25, 27, 28, 32, 34, and 35.

[0247] Preparation of tissue lysates. Epithelial tissue from the porcine gastrointestinal tract was dissected on ice. The outer mucus layer was removed by aspiration, and the rinsed tissue (with 1× phosphate-buffered saline (PBS)) was then frozen in liquid nitrogen. Tissue (10 mg) was incubated in ice-cold lysis buffer (600 μl, RIPA buffer mixed with protease and phosphatase inhibitor cocktail, Sigma-Aldrich) and homogenized to form tissue lysates. The total protein concentration of the tissue lysates was measured using the bicinchoninic acid (BCA) assay.

[0248] Catalytic activity analysis of tissue lysates. A native gel-based catalytic activity assay was performed. Proteins in tissue lysates were separated on a 7.5% non-denaturing polyacrylamide gel, and the gel was stained with gastrointestinal synthetic epithelial lining (tissue-accelerated polymerization) solution (50 ml) for 10 minutes. After staining, the gel was washed three times with PBS buffer (1x) and imaged. Figures 2A and 10.

[0249] In vitro inhibition of catalase activity. 3-Amino-1,2,4-triazole was used as an inhibitor. Tissue lysates (10 mg / ml, 100 μl) were incubated in inhibitor solution (20 mM) at 4° C. for 6 hours. See Figure 2B.

[0250] Immunoprecipitation of catalase in tissue lysates. Catalase antibodies were first conjugated to tosyl-activated magnetic beads (Dynabeads M-280) based on the Dynabeads protocol. The conjugated beads (60 mg) were then added to a tissue lysate solution (10 mg / ml, 100 μl), incubated for 2 hours to capture catalase in the solution, and placed on a magnet for 2 minutes to remove the beads. See Figure 2C.

[0251] Assessment of catalase expression in tissues. Fresh porcine tissues were collected for real-time PCR and Western blotting. Total RNA was isolated and reverse transcribed into cDNA using standard protocols. mRNA expression was measured using a LightCycler 480 II system (Roche). Catalase mRNA expression levels were normalized to housekeeping genes (β-actin, 18S, GUS, and GAPDH) and expressed as a percentage of the negative control. For Western blotting, tissue lysates were separated on SDS-PAGE gels using standard protocols. Anti-catalase (1:500 dilution in Tris-buffered saline (TBST) buffer containing Triton X 100) and anti-β-actin (1:1000 dilution in TBST buffer) were used as primary antibodies. A secondary antibody (1:2000 dilution in TBST buffer) was used for specific detection. Catalase signals were developed using SuperSignal™ West Femto Maximum Sensitivity substrate, and β-actin signals were developed using Pierce™ ECL Western blotting substrate. Western blots were imaged on a ChemiDoc™ XRS+ system (Bio-Rad) and analyzed with Image Lab 3.0. See Figures 2D-2F, 10, 11, and 12.

[0252] Microscopic analysis of polydopamine-coated tissues. Polydopamine-coated small intestines were flash-frozen and embedded in optimal cutting temperature (OCT) compound. Fixed tissues were cut into 40-μm-thick sections using a cryostat (Leica Biosystems). Specific peroxisome / catalase staining was performed as previously described (M. Connock, W. Pover, Catalase particles in the epithelial cells of the guinea-pig small intestine, Histochem. J. 2, 371–380 (1970)). Uncoated tissue sections were stained with 3,3′-diaminobenzidine (DAB) substrate or tissue-accelerating polymerization solution (1 mL) for 10 min. Slides were scanned using a digital pathology slide scanner (Leica Biosystems) and analyzed using an Aperio ImageScope (Leica Biosystems). See Figures 2G–2H, 5D, and 26.

[0253] In vivo evaluation of tissue-accelerated polymerization coating performance. All animal experiments were approved by and conducted in accordance with the Committee on Animal Care at Massachusetts Institute of Technology. A large animal model, Yorkshire pigs (Tufts, Medford, USA) weighing 45–55 kg, was selected to test the in vivo performance of tissue-accelerated polymerization coatings. The pigs were fed a daily morning and evening diet consisting of pellets (Laboratory Mini Pig Feed 5081) in addition to a midday snack consisting of fruits and vegetables. The pellets consisted of ground oats, alfalfa meal, wheat middlings, soybean meal, dried beet pulp, salt, and other micronutrient supplements. Prior to oral administration of the tissue-accelerated polymerization solution, the pigs were pre-treated with Telazol (tiletamine / zolazepam) (5 mg kg ). -1 IM), xylazine (2 mg kg -1 IM), and atropine (0.05 mg kg-1 The animals were sedated with IM (intramuscular injection), intubated, and maintained on isoflurane (1-3% via inhalation). Gastric juice was removed from the stomach before administration of Tris-buffered tissue-accelerating polymerization solution (pH 8.5). Tissue-accelerating polymerization solution (1 ml kg -1 Polydopamine was administered orally to the intestine or stomach via a catheter under endoscopic visual guidance. Gastrointestinal endoscopic videography was used to record polydopamine formation in real time after delivery of the tissue-accelerating polymerization solution, and an endoscopic camera was positioned both inside and outside the tissue-accelerating polymerization solution. To directly evaluate the polydopamine coating, pigs underwent laparotomy. A non-crushing clamp was applied to the small intestine before intestinal administration of the tissue-accelerating polymerization solution. The tissue-accelerating polymerization solution filled the intestinal lumen up to the clamp site and was unable to descend into the lower small intestine. Twenty minutes after administration, the pigs were euthanized, and tissues near the clamp were isolated, washed, and opened. All animals were euthanized before tissue collection. Macroscopic images of the tissues were taken to evaluate polydopamine coating performance. The pigs' blood pressure and heart rate were monitored throughout the procedure using a Cardell Touch® multiparameter monitor (Midmark). Additionally, no clinical or endoscopic evidence of gastrointestinal perforation, inflammation, or obstruction was observed during the study. Figures 3, 4, 14, 16, 29, 30, 31, 33, and 37.

[0254] Preparation of polydopamine probe. Barium sulfate particles (20 g) were added to Tris buffer (50 mM at pH 8.5, 1000 mL), followed by the rapid addition of dopamine (10 g). The reaction mixture was kept at room temperature with stirring (600 rpm) for 3 hours. The as-synthesized polydopamine probe was purified by centrifugation (4000 rpm × 10 min). The purified polydopamine probe was redispersed in 100 ml of water and sonicated. The purified polydopamine probe was freeze-dried for 3 days and stored at −20°C. Prior to administration, the polydopamine probe was resuspended in tissue-accelerated polymerization solution, and the tissue-accelerated polymerization solution of the polydopamine probe was used fresh. See Figures 3E and 15.

[0255] In vivo evaluation of intestinal retention of polydopamine coating through X-ray imaging. Pigs were sedated, intubated, and maintained with isoflurane as described above. Prior to administration, gastric juice was removed from the stomach. Polydopamine probes (20 mg -1 mL -1 ) was first suspended in the tissue-accelerating polymerization solution. The pigs received the tissue-accelerating polymerization solution (3 ml kg ) in which the polydopamine probe was suspended. -1 ) was administered orally and allowed to enter the small intestine. An aqueous solution containing a conventional radiopaque probe (unmodified barium sulfate particles) suspended at the same concentration was administered as a control. Radiographic examinations were performed to monitor the intestinal retention of the probe and polydopamine coating. For short-term stability evaluation, X-ray images were taken before and after rinsing the coated area with 500 ml of water. For long-term retention assessment, serial X-ray images were taken periodically at the same location at designated time points while the pigs were consistently receiving a liquid diet. In addition, the pigs were clinically and radiologically assessed for evidence of gastrointestinal perforation and obstruction (e.g., loss of appetite, abdominal distension, absence of stool, or vomiting). No clinical or radiological evidence of gastrointestinal perforation or obstruction was observed during the study. See Figures 3E-3I and 16.

[0256] In vivo coating of exogenous β-galactosidase on intestinal epithelium. Pigs were sedated, intubated, and maintained with isoflurane as described above. Gastric juice was removed from the stomach prior to administration. A laparotomy was performed on the pigs to open the small intestine. A chamber was placed on top of the intestinal epithelium, and suspended β-galactosidase (5 μg -1 mL -1 This was followed by the addition of 3 ml of tissue-accelerating polymerization solution with or without the drug (β-galactosidase and tissue-accelerating polymerization solution). Three control chambers containing solutions with and without the drug (β-galactosidase and tissue-accelerating polymerization solution) were also placed in the same pig. After 20 minutes of coating, the epithelium inside the chamber was washed three times with water, and tissue β-gal activity was assessed using o-nitrophenol-β-D-galactoside (ONPG) as a substrate. See Figures 4A-4C.

[0257] Preparation and characterization of polydopamine nano-crosslinker. Ammonium hydroxide (10 ml, 28-30% w / w) was diluted in 650 ml of ethanol-water (4:9 ratio v / v) mixture. The mixture was stirred at 30 °C for 1 h, and 50 ml of dopamine solution (50 mg in water) was added. -1 mL -1 This was followed by the addition of HCl. The reaction mixture was kept at 30°C for 24 hours. The as-synthesized polydopamine nano-crosslinker was purified by centrifugation (6000 rpm x 15 min). The purified polydopamine nano-crosslinker was redispersed in 500 ml of water and sonicated. The dry size and hydrodynamic size were measured using a transmission electron microscope (TEM, JEOL 2100F, JEOL Ltd) and a Zetasizer Nano ZS90 instrument (Malvern Panalytical), respectively. See Figures 4D-4E and 17A-17B.

[0258] Ex vivo evaluation of the blocking efficiency of tissue-accelerated polymerization. Tissues were exposed to tissue-accelerated polymerization and washed three times with PBS buffer (1x) to remove excess polydopamine. -1 mL -1 , 12.5 mg -1mL -1 , and 0 mg -1 mL -1 ) polydopamine nano-crosslinker was suspended in tissue-accelerated polymerization. The coated tissue was placed in a Franz Cell, 100 mM nutrients (CaCl, glutamate, and glucose) were added separately to the chamber (occluded with Parafilm), and samples were taken from the receptor compartment (with a stir bar) for measurement after 3 hours. See Figures 32A-32D.

[0259] In vivo evolution of an impermeable polydopamine coating to prevent glucose uptake. Pigs (fasted overnight) were sedated, intubated, and maintained with isoflurane as described above. Prior to administration, gastric juice was removed from the stomach. Polydopamine nano-crosslinker (25 mg -1 mL -1 ) was first suspended in the tissue-accelerating polymerization solution. The pigs were then given a tissue-accelerating polymerization composition (10 ml kg ) containing the nano-crosslinker suspended therein. -1) was administered orally and introduced into the small intestine. The solution was administered directly into the small intestine through a catheter under endoscopic visual guidance. For controls, 500 ml of aqueous solution was administered in the same manner. A standard oral glucose tolerance test (OGTT) was performed on the pigs 20 minutes after solution administration (Y. Lee, TE Deelman, K. Chen, DSY Lin, A. Tavakkoli, JM Karp, Therapeutic luminal coating of the intestine, Nat. Mater. 17, 834–842 (2018); E. Manell, P. Hedenqvist, A. Svensson, M. Jensen-Waern, E. Xu, Ed. Establishment of a refined oral glucose tolerance test in pigs, and assessment of insulin, glucagon, and glucagon-like peptide-1 responses, PLoS One 11, e0148896 (2016)). Pigs were given an aqueous glucose solution (3 ml kg -1 , 500mg -1 mL -1 ) was administered orally and allowed to enter the small intestine. Blood samples were collected from a central venous line at designated time points and immediately tested for glucose levels using a OneTouch Ultra® glucose monitor (LifeScan Inc.). Each data point (blood glucose change) was plotted against time, and the area under the curve was calculated for quantitative evaluation. See Figures 4A, 4D-4E, and 33.

[0260] Preparation of tissue-accelerated polymerization of praziquantel. Praziquantel particles were encapsulated in polydopamine by utilizing reactive groups (e.g., catechol and amine groups) on the polydopamine surface, which allowed for chemical crosslinking and incorporation of praziquantel particles into the polydopamine coating layer. Additionally, the hydrophilic polydopamine layer on the particle surface dramatically improved the stability and dispersion properties of hydrophobic drug particles. Praziquantel particles (powder) (8 g) were sieved (150–300 μm mesh size) and added to 400 ml of Tris buffer (50 mM, pH 8.5), followed by the rapid addition of dopamine (4 g) (J. Park, TF Brust, HJ Lee, SC Lee, VJ Watts, Y. Yeo, Polydopamine-based simple and versatile surface modification of polymeric nano drug carriers, ACS Nano 8, 3347–3356 (2014)). The reaction mixture was kept at room temperature and stirred (600 rpm) for 3 hours. The as-synthesized praziquantel particles were purified by centrifugation (4000 rpm x 10 min). The purified praziquantel particles were freeze-dried for 3 days and stored at -20°C. Before administration, the praziquantel particles were resuspended in tissue-accelerated polymerization solution, and the praziquantel tissue-accelerated polymerization solution was used fresh. See Figures 4A, 4F, and 4G.

[0261] Assessment of praziquantel concentrations. High-performance liquid chromatography was performed using an Agilent 1260 Infinity II HPLC system (Agilent Technologies, Inc.) equipped with a Model 1260 Quaternary Pump, Model 1260 Hip ALS Autosampler, Model 1290 Thermostat, Model 1260 TCC Control Module, and Model 1260 Diode Array Detector. Data processing and analysis were performed using OpenLab CDS® software (Agilent Technologies, Inc.). For praziquantel, an Agilent Zorbax Eclipse XDB C system maintained at 40°C was used. -1 Chromatographic isocratic separations were performed on a 4.6 × 150 mm analytical column (with 5 μm particles). The optimized mobile phase was 1 ml min over a 5 min run time. -1 The elution mixture consisted of MilliQ-grade water and acetonitrile at a flow rate of 0.05 sq. m / s. Separation was achieved using a gradient elution profile starting with 50% water and 50% acetonitrile at 0 min and ending with 30% water and 70% acetonitrile at 3 min. The injection volume was 5 μl, and the selected ultraviolet (UV) detection wavelength was 217 nm. See Figures 4F and 4G.

[0262] In vivo evolution of the pharmacokinetics of praziquantel tissue-accelerated polymerization. Pigs were sedated, intubated, and maintained with isoflurane as described above. Prior to administration, gastric juice was removed from the stomach. Praziquantel tissue-accelerated polymerization (20 mg -1 mL -1 , 1ml kg -1 ) was delivered into the small intestine. Praziquantel without tissue-accelerated polymerization was used as a control. Blood samples were collected from the marginal ear vein at the indicated time points. Serum samples were separated from the blood by centrifugation (1800G x 10 minutes at 4°C) and stored at -80°C for further analysis. See Figures 4F and 4G.

[0263] Assessment of serum praziquantel concentrations. Praziquantel concentrations in serum from in vivo experiments were analyzed using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS / MS). Analyses were performed on a Waters ACQUITY UPLC-I-Class System aligned with a Waters Xevo TQ-S mass spectrometer (Waters Corporation, Milford, MA). Liquid chromatographic separation was performed on an Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm particle size) column at 50°C. The mobile phase consisted of an aqueous 0.1% formic acid, 10 mM ammonium formate solution (mobile phase A) and an acetonitrile:10 mM ammonium formate, 0.1% formic acid solution (95:5 v / v) (mobile phase B). The mobile phase had a continuous flow rate of 0.6 ml / min using a time and solvent gradient composition. For the analysis of praziquantel, the initial composition of 80% mobile phase A was held for 0.50 minutes, followed by a linear change in composition to 0% mobile phase A over the next 2.00 minutes. The composition of 0% mobile phase A and 100% mobile phase B was held constant until 3.50 minutes. At 3.51 minutes, the composition returned to 80% mobile phase A and remained at this composition until the completion of the run, which ended at 5.00 minutes, where it remained for column equilibration. The total run time was 5.00 minutes. The mass-to-charge transitions (m / z) used to quantify praziquantel were 313.22 > 203.09 and 313.22 > 83.01 for quantification and confirmation, respectively. As an internal standard, mebendazole, 296.06>264.03 and 296.06>76.99 m / z transitions were used for quantification and confirmation, respectively. Sample introduction and ionization were by electrospray ionization (ESI) in positive ionization mode. Waters MassLynx 4.1 software was used for data acquisition and analysis. A stock solution of praziquantel was prepared in methanol at a concentration of 500 μg / ml.A 12-point calibration curve was prepared in blank serum containing no analyte, ranging from 1.25 to 5000 ng / ml. 100 μl of each serum sample was spiked with 250 ng / ml mebendazole in 200 μl of acetonitrile to induce protein precipitation. The samples were vortexed, sonicated for 10 minutes, and centrifuged at 13,000 rpm for 10 minutes. 200 μl of the supernatant was pipetted into a 96-well plate containing 200 μl of water. Finally, 1.00 μl was injected onto the UPLC-ESI-MS system for analysis. See Figures 4F and 4G.

[0264] Assessment of serum and tissue dopamine concentrations. Dopamine concentrations in serum and tissues from in vivo experiments were analyzed using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS / MS). Analyses were performed on a Waters ACQUITY UPLC-I-Class System aligned with a Waters Xevo TQ-S mass spectrometer (Waters Corporation, Milford, MA). Liquid chromatographic separation was performed on an Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm particle size) column at 50 °C. The mobile phase consisted of an aqueous 0.1% formic acid, 10 mM ammonium formate solution (mobile phase A) and an acetonitrile:10 mM ammonium formate, 0.1% formic acid solution (95:5 v / v) (mobile phase B). The mobile phase had a continuous flow rate of 0.45 ml / min using a time and solvent gradient composition. Sample introduction and ionization were by electrospray ionization (ESI) in positive ionization mode. Waters MassLynx 4.1 software was used for data acquisition and analysis. Stock solutions were prepared in methanol at a concentration of 500 μg / ml. A 12-point calibration curve was prepared in blank porcine serum containing no analyte, ranging from 1.25 to 10,000 ng / ml. 100 μl of each serum sample was spiked with 500 ng / ml methyldopamine (internal standard) in 100 μl of acetonitrile to induce protein precipitation. 200 μl of 5 mg / ml fluorescamine in acetonitrile was then added to each sample as a derivatizing agent for both dopamine and methyldopamine to aid in detection. Samples were vortexed, sonicated for 10 minutes, and centrifuged at 13,000 rpm for 10 minutes. 300 μl of the supernatant was incubated for 60 minutes at 37°C. 200 μl of the incubated solution was then added to 200 μl of water in a 96-well plate. Finally, 10 μl was injected onto a UPLC-ESI-MS system for analysis. See Figure 31.

[0265] Tissue samples were divided into approximately 300 mg pieces. 3% bovine serum albumin in PBS buffer was added at a volume-to-mass ratio of 2:1. The samples were homogenized at 4°C. 100 μl of each homogenate was spiked with 500 ng / ml methyldopamine in 100 μl acetonitrile and 5 mg / ml fluorescamine in 200 μl acetonitrile for derivatization. 1.00 ml of ethyl acetate was also added to the homogenized samples for extraction. The samples were vortexed, sonicated for 10 minutes, and centrifuged at 13,000 rpm for 10 minutes. Following centrifugation, 300 μl of the supernatant was incubated at 37°C for 60 minutes. The samples were allowed to evaporate overnight. The evaporated samples were reconstituted with 300 μl acetonitrile and centrifuged at 6,000 rpm for 5 minutes. 200 μl of the supernatant was pipetted into a 96-well plate containing 200 μl of water. Finally, 10 μl was injected onto the UPLC-ESI-MS system for analysis. See Figure 31.

[0266] For the analysis of dopamine fluorescamine, the initial composition, 95% mobile phase A, was held for 0.75 min. Following this, the composition was linearly changed to 5% mobile phase A and 95% mobile phase B until 1.00 min. The composition was held constant at 95% mobile phase B until 3.00 min. At 3.25 min, the composition returned to 95% mobile phase A, where it remained for column equilibration for the run period that ended at 4.00 min. The mass-to-charge transitions (m / z) used to quantify dopamine were 414.223 > 137.125 and 414.223 > 119.115 for quantification and confirmation, respectively. For the internal standard, methyldopamine fluorescamine, the m / z transitions 472.223 > 139.139 and 472.223 > 278.094 were used for quantification and confirmation, respectively. See Figures 31A and 31B.

[0267] Cytotoxicity assay. The cytotoxicity of polydopamine (PDA) in live cells was tested using the Vybrant MTT cell proliferation assay. As-prepared polydopamine was added to cell culture medium at various concentrations (0, 10, 50, 250, 500, 1000, 1500, and 2000 μg / ml). Cytotoxicity was tested against several cell lines: HeLa (ATCC), COLO320DM (ATCC), Caco-2 (ATCC), Hep3B (ATCC), and HS 895.T (ATCC) by seeding them into 96-well plates at a density of 10,000 cells each and maintaining them in medium for 24 hours before replacing the medium with 100 μl of polydopamine solution. The cells were incubated at 37°C in a cell culture incubator for various times (6, 12, and 24 hours). The cells were washed three times with PBS buffer, followed by the addition of MTT solution (10 μl) and cell culture medium (100 μl) to each well and incubation at 37° C. for 4 hours. After 4 hours, 100 μl SDS-HCl solution was added to each well for another 4 hours of incubation (at 37° C.). The absorbance at 570 nm was recorded on a 96-well plate reader (Tecan Infinite M200). See FIG. 19.

[0268] Oral toxicity test. According to the guidelines published by the OECD with minor modifications (OECD, Test no. 407: repeated dose 28-day oral toxicity study in rodents OECD Guidel. Test. Chem. Sect. 4, OECD Publ. Paris, 1-10 (2008)), three groups (four animals per group) of rats (Sprague Dawley, 150-200 g, Charles River Labs) were separately administered water (5 ml kg -1 ), as-prepared polydopamine (15 mg mL -1 , 5ml kg -1 ), and tissue-accelerating polymerization solution (5 ml kg-1 ) for a period of 4 weeks. The solution was administered directly to the rats through a gavage needle rather than an endoscopic catheter. Body weight was measured daily for 28 days. Blood samples were collected on day 27 for hematological and blood biochemistry measurements, and food was withheld for 24 hours. On day 28, all 12 rats were euthanized and necropsied. Samples of the heart, lung, liver, kidney, spleen, stomach, small intestine, and large intestine were collected for histological analysis. See Figures 20, 21, 22, and 23.

[0269] Histological analysis of tissues. Tissues were first fixed in PBS buffer (1x) with 4% paraformaldehyde for 6 hours and then placed in PBS buffer with 30% sucrose overnight at 4°C. Fixed tissues were embedded in paraffin and cut into 5 μm-thick sections using a cryostat (Leica Biosystems). Sections were stained with hematoxylin and eosin for histopathological analysis. See Figures 5D and 23.

[0270] Polydopamine coating of impermeable polycarbonate sheets. The polycarbonate sheets were exposed to tissue-accelerating polymerization solution (without H2O2) for 36 hours and washed with water to remove excess polydopamine. See Figures 36A-36C.

[0271] Statistical analysis. All data were reported as mean ± SD for n > 3 measurements for each group. Significance was determined using two-sample t-tests, one-way ANOVA, and post-hoc Bonferroni multiple comparison tests.

[0272] Overview of Selected Examples The compositions, methods, and kits described in this disclosure have been evaluated for their therapeutic value in several clinical scenarios. Tissue-accelerated polymerization was applied to address lactose intolerance by coating digestive enzymes on the small intestinal epithelium. Results showed that β-galactosidase coated on the tissue improved the efficiency of lactose digestion by approximately 20-fold, suggesting the therapeutic utility of this disclosure in digestive disorders and diseases. Furthermore, the power of tissue-accelerated polymerization technology was assessed in regulating intestinal glucose absorption, an urgent need for patients with type 2 diabetes. An impermeable coating layer acting as a glucose-blocking barrier has been developed to prevent postprandial glucose uptake (reducing the glucose response by approximately 70%). The application of tissue-accelerated polymerization has been extended to improve the administration efficiency of drug treatments with inconvenient regimens, demonstrating its ability to sustain the release of therapeutic agents. The disclosed method for administering praziquantel (an anthelmintic drug) achieves long-lasting (over 24 hours) drug levels in the systemic circulation (a 10-fold increase in half-life values), potentially revolutionizing drug treatment options for patients with schistosomiasis and other diseases dependent on strict management. Coupled with the outstanding performance of the tissue-accelerated polymerization technology in human tissue specimens, these clinical applications are suitable for in vivo human testing. The tissue-accelerated polymerization technology is applicable in a wide range of technology adoptions and applications in disease treatment and healthcare.

[0273] Example 1 Endogenous enzyme-catalyzed polydopamine growth on epithelia. To demonstrate the acceleration of polydopamine polymerization by catalase in a hypoxic environment mimicking the low partial pressure of oxygen in the gastrointestinal tract, we quantitatively evaluated the rate of polydopamine polymerization under different reaction conditions while maintaining the same dopamine concentration (Figures 1B-D and 6A-6B). In an extremely oxygen-depleted environment, the slow conventional polydopamine polymerization was inhibited by 65% ​​(Figures 6A-6B). Addition of trace amounts of hydrogen peroxide (HO), which acts as a strong reducing agent, prevented dopamine oxidation and nearly quenched the reaction. As shown in Figure 1C, the gradual color change of the dopamine solution from clear to light gray over 2 h suggests minimal polydopamine polymerization, and a clear dopamine-HO solution suggests negligible polydopamine polymerization. In contrast, the color of the dopamine solution with the addition of both catalase and HO rapidly changed to dark brown, demonstrating accelerated polydopamine polymerization. In this study, we confirmed that the catalase-catalyzed polymerization product from both commercially purified enzyme and crude tissue lysates was polydopamine using both Fourier transform infrared (FTIR) and ultraviolet-visible (UV-Vis) spectroscopy (Figures 7A-7D and 8A-8C). To further quantify the effect of catalase on polydopamine polymerization rate, we plotted a comparison of the reaction kinetics by measuring the solution optical attenuation at 700 nm, where polydopamine has optical absorption properties (Figure 1D). The signal for the catalase-polydopamine combination grew rapidly and plateaued within 10 minutes of reaction. However, while the intensity of the optical attenuation was relatively low in the other conditions, even after 2 hours, it reached the same intensity level within 20 seconds under catalase catalysis, indicating an approximately 400-fold increase in polymerization rate.

[0274] Next, we assessed whether endogenous catalase could promote polydopamine polymerization and coating on the small intestine to achieve tissue-accelerated polymerization. Due to its anatomical and physiological similarities with the human digestive system, the porcine gastrointestinal tract was chosen as the initial model tissue. Ex vivo tissue was incubated with a tissue-accelerated polymerization solution containing dopamine and hydrogen peroxide at concentrations within safe oral intake levels. As shown in Figure 1E and Figure 9, when the mucosal side of the small intestine was exposed to the tissue-accelerated polymerization solution, a dark brown polydopamine coating was clearly observed on the epithelial surface, demonstrating that polydopamine was formed in situ and deposited on tissue anchor points due to the high reactivity between polydopamine and primary amines in nearby biomolecules. 14 In contrast, almost no polydopamine was found on the serosal side of the tissue after the same incubation period, suggesting that polydopamine polymerization is an enzyme-dependent reaction (Figure 1E). These polydopamine coating processes were visualized due to the chromogenic properties of polydopamine. To further quantify the rate of polydopamine growth, the amount of polydopamine coated on the small intestine was analyzed over a range of reaction times. The coating kinetics showed rapid polydopamine signal generation, reaching completion within 12 min (Figure 1F), suggesting a fast polydopamine coating process. In addition to efficiency, the specificity of tissue-accelerated polymerization was also evaluated by comparing tissue-accelerated polymerization coating in different parts of the gastrointestinal tract (including the esophagus, stomach, duodenum, jejunum, ileum, and colon). As shown in Figure 1G, tissue images (6 mm diameter) revealed distinct polydopamine coatings on the duodenum and jejunum, relatively little polydopamine coating on the ileum and colon, and negligible polydopamine coating on the stomach and esophagus. This coating pattern was further substantiated through quantitative polydopamine signal analysis in Figure 1H. The exceptional efficiency and specificity of this tissue-accelerated polymerization paves the way for downstream in vivo applications.

[0275] Example 2 Mechanisms of tissue-accelerated polymerization at the molecular, cellular, and tissue levels Prior to exploring the application of the tissue-accelerated polymerization technique, we systematically investigated its biological mechanism in detail. First, we tested whether endogenous catalase is a determining factor in catalytic polydopamine polymerization on epithelia. Pig gastrointestinal epithelial tissue was excised and washed, the outer mucus layer was removed by aspiration, and then tissue lysates were prepared by homogenizing the rinsed tissue, which was further diluted relative to the total protein concentration. These tissue lysates were individually added to the tissue-accelerated polymerization solution, and the optical attenuation of each solution was measured following the reaction. As shown in Figure 2A, the signal of the polydopamine solution using the small intestinal epithelial lysate was higher than that of the others, consistent with the tissue coating results (Figure 1G). In addition, a similar trend was observed in the analysis of the catalytic ability of these lysates through native gel electrophoresis and polydopamine staining (Figure 10). Only one sharp band was visualized in each lane, supporting the predicted role of catalase as the sole enzyme involved in polydopamine polymerization. To further confirm the exclusive role of catalase, small intestinal lysates were treated with either a catalase-specific inhibitor to reduce catalase activity or magnetic beads coated with catalase antibodies to remove immunoprecipitated catalase. As shown in Figure 2B, the catalytic activity of the lysates was reduced by 80% after adding the inhibitor and by 90% upon removal of catalase from the samples (Figure 2C), demonstrating that the tissue-accelerated polymerization process is exclusively catalase-dependent. Additionally, it was also confirmed that catalase present due to bacteria in the small intestinal mucus did not affect the tissue-accelerated polymerization coating process (Figures 24A-24B and 25), indicating that the majority of HO was not degraded or consumed in the intestinal lumen and that the amount of HO was sufficient to activate oxygen release and polydopamine polymerization. Furthermore, catalytic polydopamine polymerization was observed to occur primarily in the small intestine, which is due to the higher expression of catalase in the small intestine compared to other segments of the GI tract.

[0276] It has previously been shown that the expression of human catalase at mRNA and protein levels is higher in the small intestine than in other organs of the gastrointestinal tract, such as the esophagus, stomach, and large intestine. 15 This plays a role in the coating specificity of the tissue-accelerated polymerization technique. To further confirm this distinct catalase expression characteristic in the gastrointestinal epithelium, quantitative evaluation of catalase expression along the porcine gastrointestinal tract was performed by both gene and protein analysis. As shown in Figure 2D, high levels of catalase activity were detected in the duodenum and jejunum, while catalase activity levels were relatively low in the esophagus, stomach, ileum, and colon, suggesting stronger catalase activity in the small intestine compared to other gastrointestinal tissues. In addition, catalase mRNA levels in tissues were measured using quantitative real-time polymerase chain reaction (PCR). Catalase mRNA expression levels in the small intestine were approximately 10-fold higher than those in other epithelial tissues (Figure 2E and Figures 11A-11B), yet there was no significant difference in the mRNA levels of control genes (housekeeping genes) between the small intestine and other organs (Figures 12A-12E). As expected, small intestinal catalase protein expression levels had a similar distribution profile compared with mRNA expression levels in the gastrointestinal tract (Western blot analysis, Figure 2F).

[0277] Efforts were then made to microscopically characterize the interface between the polydopamine layer and the epithelium. Chromogenic polydopamine was visualized not only by the naked eye but also by microscopy. 16Interestingly, during the tissue-accelerated polymerization process, polydopamine was first found to be deposited on the tips of intestinal villi and then coat the entire villi and surrounding areas (Figure 13). Microscopic analysis revealed a thin polydopamine layer firmly coated on the external villi (Figure 2G), but none on control tissue (without tissue-accelerated polymerization treatment). Polydopamine was completely confined to the luminal surface, the outer layer of the epithelium, rather than inside the epithelial cells, demonstrating that polydopamine polymerization occurs exclusively at the epithelial surface, leaving dopamine outside the cells throughout the entire reaction (Figures 26A-26C, 27, and 28). Specific peroxisome / catalase staining studies showed that catalase is located inside peroxisomes, which have a dense distribution in the villi but not in the submucosa and other internal layers (Figure 2H, left panel). Similar studies have confirmed that catalase "particles" are present in the peroxisomes of intestinal epithelial cells. 17 To further confirm that peroxisomal catalase can catalyze polydopamine formation, we used dopamine for staining instead of typical peroxisomal / catalase substrates. As shown in Figure 2H (right panel), dark brown polydopamine spots were observed inside epithelial cells, and the staining pattern was consistent with conventional peroxisomal / catalase staining, confirming the catalytic ability of peroxisomal catalase for polydopamine polymerization in sectioned tissue segments. In tissue-accelerated polymerization-coated samples that underwent tissue-accelerated polymerization luminally and were subsequently sectioned for microscopy without peroxisomal / catalase staining, no peroxisomal polydopamine patterns were observed (Figure 2G, left panel).

[0278] Example 3 Evaluation of tissue coating performance based on tissue-accelerated polymerization in vivo Where the biological mechanism of tissue-accelerated polymerization has been demonstrated, the in vivo performance of this tissue coating technology was tested in Yorkshire pigs. Again, it is worth mentioning that the advantages of using pigs as a large animal model include their extensive homology with the human genome, anatomical similarity with the human gastrointestinal tract, and physiological similarity with the human digestive system. 18、19 Under moderate sedation, pigs were orally administered the tissue-accelerating polymerization solution and allowed to pass through the esophagus into the small intestine under endoscopic monitoring (Figure 3A). Gastrointestinal endoscopy was used to record polydopamine formation in real time after delivery of the tissue-accelerating polymerization solution, and an endoscopic camera was placed both inside and outside the tissue-accelerating polymerization solution (Figures 3B and 3C). The in vivo observations were consistent with those of the ex vivo tissue coating study. As shown in Figure 3C, a dark brown polydopamine layer was observed on the small intestinal wall 20 minutes after administration, whereas no such polydopamine coating was visualized in the stomach after the same gavage (Figures 14A-14C). The tissue-accelerating polymerization solution remained generally stable in the stomach for 30 to 60 minutes (Figure 29), confirming the durability of the solution. Of note, all animals were fasted overnight, and gastric juice was removed from the stomach before administration of the Tris-buffered tissue-accelerated polymerization solution (pH 8.5), thereby minimizing the possible effect of pH on the stability of the tissue-accelerated polymerization solution and polydopamine polymerization. Additionally, microscopy (Figures 26A-26C), UV-Vis spectroscopy (Figure 30), and liquid chromatography-tandem mass spectrometry (Figures 31A-31B) were applied for signal measurement, and no detectable increase in polydopamine or dopamine signals was observed in the submucosa and blood after tissue-accelerated polymerization coating. Furthermore, no significant increase in blood pressure or heart rate was observed in any of the animals throughout the entire procedure, demonstrating the lack of dopamine absorption and supporting the early safety of the tissue-accelerated polymerization technique. 40Consistent with the schematic illustration of the tissue-accelerated polymerization technique depicted in Figure 1A, the endoscopic video revealed that the polydopamine coating process involved three major steps. When the clear dopamine-HO solution was first introduced into the small intestinal lumen, oxygen bubbles formed, and the intestinal wall rapidly turned a light yellow-brown color, suggesting the rapid diffusion of hydrogen peroxide and the initiation of polydopamine polymerization. Second, increasing amounts of oxygen bubbles were generated at the epithelium-solution interface (inner camera view) and released into the solution (outer camera view), confirming the rapid decomposition of hydrogen peroxide and providing evidence for the mechanism of oxygen release. Third, the interfacial oxygen catalyzed polydopamine polymerization and adhesion to the intestinal epithelium, while the tissue-accelerated polymerization solution remained liquid and the intestinal lumen remained enlarged, mitigating concerns about intestinal adhesions and obstruction. The results show that after coating, the solution inside the lumen also turned dark brown, probably due to the diffusion of unbound polydopamine into the solution.

[0279] To directly evaluate coating formation, pigs underwent laparotomy. A non-crushing clamp was applied to the small intestine prior to endoscopic administration of the tissue-accelerated polymerization solution into the intestine. As shown in Figure 3D, the tissue-accelerated polymerization solution filled the intestinal lumen up to the clamp site and was unable to penetrate down into the lower small intestine. The tissue near the clamp was isolated, washed, and opened. Different polydopamine coatings were observed before and after the clamp site. Compared to the control segment after the clamp site, the tissue submerged in the tissue-accelerated polymerization solution showed an improved polydopamine coating density, confirming the significant tissue-coating performance of tissue-accelerated polymerization in vivo.

[0280] We next investigated whether tissue-accelerated polymerization technology could enable safe and prolonged intestinal retention. Polydopamine shedding from the epithelium was visualized endoscopically after 24 hours, suggesting that the coating was transient. However, given the inconvenience of the endoscopic procedure, the frequent incompatibility of sedation, and the uncertainty of solutions remaining in the field of endoscopic imaging, a more ergonomic method is needed to monitor polydopamine intestinal retention. X-ray imaging is a commonly used, convenient, and effective tool for examining the gastrointestinal tract. 19 To apply X-ray imaging to this study, we modified conventional X-ray contrast agents by encapsulating radiopaque particles in polydopamine (Figure 15A). These polydopamine probes, suspended in a tissue-accelerating polymerization solution, were co-coated with polydopamine onto the intestinal epithelium and incorporated into the polydopamine coating layer, enabled by chemical cross-linking between reactive polydopamine on the probe surface and dopamine monomers or oligomers in the tissue-accelerating polymerization solution (Figure 3E). 14This co-coating performance was first characterized through ex vivo studies (Figures 15B and 15C). The coated polydopamine probe was easily visualized through X-ray imaging, and no signal decay was observed after rinsing the epithelial surface with water, demonstrating the stability of the polydopamine probe coating. In contrast, no significant X-ray signal was detected in control experiments in which a conventional probe or polydopamine alone was applied for coating. The polydopamine probe demonstrated its potential for in vivo imaging of the intestinal retention of polydopamine coating layers. When a tissue-accelerating polymerization solution containing the suspended polydopamine probe was administered to healthy pigs according to the clinical procedure of the barium meal test, X-ray signal enhancement was observed in the small intestine (Figure 16A), revealing the shape of the intestine. However, when an equivalent concentration of the conventional probe was administered to the pigs, the resulting X-ray signal of the small intestine was weaker in comparison. It is also worth mentioning that after rinsing the imaging area, the signal from the conventional probe was barely detectable, whereas the polydopamine probe remained clearly visualized (Figure 3G), with an 8.9-fold difference in signal intensity between the two probes (Figure 3H), demonstrating efficient incorporation of the polydopamine probe and stable coating of polydopamine on the tissue. To monitor intestinal polydopamine retention over time, serial X-ray images were taken periodically at the same location (Figure 3G and Figure 16B). The animals were consistently fed a liquid diet throughout imaging, mimicking realistic conditions and testing the stability of the polydopamine coating in the presence of food. Quantitative signal intensity analysis was performed on the small intestine and surrounding tissues (Figure 3I). When the conventional probe was administered to pigs, the X-ray signal in the small intestine was barely detectable after rinsing and 2–24 h of exposure to food, due to relatively weak tissue adhesion that caused rapid attenuation of the probe.However, when polydopamine probes were administered for imaging, a clear X-ray signal was observed in the small intestine after 2 hours of exposure to food, which decreased by only 28% at 6 hours, indicating prolonged intestinal retention of the polydopamine coating. The reduced polydopamine signal is likely due to the rapid turnover of intestinal mucus through goblet cell secretion (approximately 12–24 hours) and the frequent turnover of the epithelium through stem cell proliferation (approximately 1–5 days). 20 This is due to the exfoliation of the polymeric coating layer and the epithelium underlying the polydopamine into the lumen. Notably, the intestinal X-ray signal fell back to pre-administration levels after 24 hours, suggesting complete decay and transient retention of the polydopamine coating layer. This confirmation of the transient nature of the polydopamine coating suggests the safety of the tissue-accelerated polymerization technique.

[0281] Example 4 Tissue-accelerated polymerization for the regulation of enzymatic digestion To demonstrate the versatility of the tissue-accelerated polymerization technique, we evaluated its therapeutic value in modulating enzymatic digestion (Figure 4A). Polydopamine is highly reactive with numerous chemical groups, including amine, phenol, and sulfhydryl groups, allowing for the facile incorporation of functional agents into the polydopamine coating layer. 14 Digestive enzymes were therefore incorporated into the tissue-accelerating polymerization system.

[0282] We utilized tissue-accelerated polymerization technology to improve digestive efficiency by incorporating digestive enzymes into a polydopamine-coated layer on the small intestinal epithelium (Figure 4B). Recent studies have shown that approximately 70% of the world's population has hypolactosis, which refers to low or absent levels of lactase, leading to lactose intolerance. 21、22 If tissue-accelerated polymerization technology were used to coat exogenous β-galactosidase on the intestinal epithelium, it would enhance lactose digestion in the small intestine. Restoring the function of intestinal brush border enzymes, especially β-galactosidase, would enable the treatment of digestive disorders and diseases, improving patient prognosis and quality of life.23、24 .

[0283] To demonstrate the ability of tissue-accelerated polymerization technology to improve lactose digestion, we used a tissue-accelerated polymerization solution with suspended β-galactosidase to coat the small intestine of a sedated pig, where a laparotomy was performed to provide access to the small intestinal mucosa. β-galactosidase activity of the coated epithelium was then assessed after rinsing. Coating efficiency was confirmed through analysis of β-galactosidase activity with and without the agent (β-galactosidase and tissue-accelerated polymerization solution). As shown in Figure 4C, quantitative comparison of β-galactosidase activity suggested an approximately 20-fold increase in digestion efficiency upon addition of the tissue-accelerated polymerization-based β-galactosidase coating to the pig small intestine. In contrast, no improvement in β-galactosidase activity was detected in the control groups (no β-galactosidase, no tissue-accelerated polymerization solution, or no both), confirming that the increase in β-galactosidase activity was due to its pairing with the tissue-accelerated polymerization technology. Additionally, when the tissue-accelerated polymerization solution alone was administered, no reduction in digestive enzyme activity was observed compared to uncoated tissue. This consistent baseline enzyme activity suggests that neither the coating chemistry (crosslinking between polydopamine and epithelial biomolecules) nor the coated polydopamine itself inhibited the intrinsic digestive enzyme activity of the epithelium, demonstrating the permeability of polydopamine in the small intestine, allowing molecules to pass through by diffusion. The tissue-accelerated polymerization technology opens new avenues for enhancing digestive efficiency in the small intestine and as a promising mechanism for treating digestive disorders and diseases.

[0284] Example 5 Accelerated tissue polymerization for the regulation of nutrient absorption The therapeutic value of the technology in modulating nutrient absorption was also evaluated (Figure 4A). Polydopamine is highly reactive with numerous chemical groups, such as amine, phenol, and sulfhydryl groups, allowing for the facile incorporation of functional agents into the polydopamine coating layer. 14Nutrient blockers were therefore incorporated into the tissue-accelerating polymerizing system to develop an impermeable barrier to regulate nutrient absorption.

[0285] Glucose absorption in the small intestine plays a role in regulating plasma glucose levels, which are commonly associated with metabolic and systemic diseases such as obesity, hyperinsulinemia, and diabetes. 25 To prevent excess glucose uptake in patients with these diseases and disorders, procedures such as gastric bypass surgery, placement of an intestinal sleeve, surgical adhesives, and gastrointestinal electrical stimulation are being tested. 3、7、26、27 However, these procedures are invasive and target the entire gastrointestinal tract, limiting their widespread adoption. Tissue-accelerated polymerization technology offers a non-invasive, tissue-targeted method for modulating glucose absorption. The power of tissue-accelerated polymerization technology has been demonstrated in isolating nutrient exposure, particularly glucose absorption, in the small intestine.

[0286] Using this technique, we coated small intestinal epithelium with an impermeable polydopamine coating layer to provide a barrier against glucose uptake (Figure 4D). To produce a highly crosslinked polydopamine coating layer with ultralow permeability, nano-crosslinkers were added to the tissue-accelerated polymerization solution. As shown in Figures 17A-17B, the additional nano-crosslinkers were approximately 527 nm in diameter and had a uniform size distribution. These hydrophilic nano-crosslinkers suspended in the tissue-accelerated polymerization solution were then incorporated into the polydopamine coating layer, which was made possible by chemical crosslinking between the reactive catechol and amine groups exposed on the surface of the nano-crosslinkers and the dopamine monomers or oligomers in the tissue-accelerated polymerization solution. Thus, the incorporation of the crosslinkers increases the number of covalent bonds within the polydopamine coating, enabling a highly compact polydopamine coating layer with low permeability. To verify the nutrient regulation function, the tissue-accelerated polymerization solution with suspended nano-crosslinkers was administered to pigs via endoscopy. Following tissue-accelerated polymerization coating, the pigs were given oral glucose, followed by a standard oral glucose tolerance test (OGTT). Blood glucose levels were monitored to quantify intestinal glucose absorption. As shown in Figure 4E, control pigs without the tissue-accelerated polymerization coating showed dramatically increased blood glucose levels after oral glucose administration. However, animals treated with the tissue-accelerated polymerization solution showed a reduced blood glucose response. A quantitative comparison of blood glucose levels in samples from pigs with and without the tissue-accelerated polymerization coating, based on six independent experiments performed and analyzed on six different pigs, showed that blood glucose levels in the tissue-accelerated polymerization coating were reduced by an average of 73.3% compared to the control. This reduction in glucose response demonstrated the ability of the tissue-accelerated polymerization technology to effectively prevent postprandial glucose uptake. In addition, the tissue-accelerated polymerization barrier showed efficient blocking ability of different nutrients, and the blocking efficiency was adjusted by adjusting the crosslink density of the tissue-accelerated polymerization coating layer (Figures 32A-32D).Furthermore, we demonstrated that the tissue-accelerated polymerization coating layer was transient, restoring normal glucose absorption after 24 hours (Figure 33). These results address the clinical need to noninvasively block glucose absorption in the small intestine and demonstrate the feasibility of the tissue-accelerated polymerization technology as a therapeutic approach for patients with type 2 diabetes.

[0287] Example 6 Tissue-accelerated polymerization for controlled drug release. The therapeutic value of the technology in modulating drug release was also evaluated (Figure 4A). Polydopamine is highly reactive with numerous chemical groups, such as amine, phenol, and sulfhydryl groups, allowing for the facile incorporation of functional agents into the polydopamine coating layer. 14 Anthelmintic drugs were therefore incorporated into the tissue-accelerated polymerization system, and the system was evaluated for use in oral drug delivery, demonstrating its ability for sustained release of therapeutic agents in the small intestine.

[0288] The development of oral sustained-release drugs is limited by the rapid transit time of therapeutic agents in the gastrointestinal tract. Attempts to extend the gastrointestinal residence of drugs, particularly in the small intestine, which is a more compatible environment for sensitive pharmaceuticals compared to the harsh acidity of the stomach, have been made with little success. 19、28 Praziquantel was chosen as a model drug to test the ability of tissue-accelerated polymerization technology to prolong intestinal retention. Praziquantel is the only anthelmintic drug frequently used to treat schistosomiasis, a major neglected tropical disease caused by parasitic worms that affects more than 200 million people worldwide. 29、30 Praziquantel has a half-life of 1 to 1.5 hours in humans, so it is recommended to take it orally three times daily with a 5-hour interval, a strict regimen that is difficult to adhere to. Alternative technologies that allow for prolonged intestinal retention to reduce dosing frequency are urgently needed.

[0289] As shown in Figure 4F, praziquantel particles (powder) were coated onto the small intestinal epithelium using tissue-accelerated polymerization technology, enabling its prolonged intestinal retention and sustained release. To incorporate the drug into the polydopamine coating layer, praziquantel particles (powder) were encapsulated in polydopamine. A pharmacokinetic study of a single oral dose of praziquantel tissue-accelerated polymerization solution in pigs was conducted to investigate the release kinetics of praziquantel tissue-accelerated polymerization solution. After administration, blood samples were collected at 0, 1, 2, 3, 4, 5, 6, 24, and 48 hours and further analyzed for serum praziquantel concentrations by liquid chromatography-tandem mass spectrometry. A quantitative comparison of the pharmacokinetics, shown in Figure 4G, revealed that the retention time of praziquantel using tissue-accelerated polymerization solution was extended compared to conventional administration. When praziquantel controls (without tissue-accelerated polymerization solution) were administered conventionally, the drug was rapidly cleared from the blood with a decay phase of 2 to 6 hours, and no drug was detectable at or after 24 hours. However, blood levels of praziquantel administered via the tissue-accelerated polymerization technique persisted for longer than 6 hours and were still detectable at 24 hours (20.0 ng mL). -1 This was accompanied by a mean concentration of 15.1 ng mL -1 ), was even higher than that of praziquantel (905.8 ng h mL). A non-compartmental pharmacokinetic model was used to perform quantitative analysis of pharmacokinetic parameters, including area under the curve (AUC) and half-life. Results from three large animal studies showed that praziquantel (905.8 ng h mL -1 , with tissue-accelerating polymerization solution) and praziquantel control (222.4 ng h mL -1 ) showed a 4-fold increase between the AUC values. In addition, the half-life of praziquantel increased 10-fold (from 1.3 to 13 hours) when tissue-accelerated polymerization technology was used, demonstrating a prolonged intestinal residence of the drug. Of note, praziquantel is primarily metabolized by cytochrome P450 enzymes, CYP3A4, which is specifically present in the small intestine and liver. 30、38To confirm that the tissue-accelerated polymerization coating did not affect drug metabolism, CYP3A4 activity in epithelia with and without a polydopamine coating layer was measured and compared (Figure 34), and no change in CYP3A4 activity was observed after the tissue-accelerated polymerization coating. The sustained release of praziquantel through tissue-accelerated polymerization technology expands the availability of treatment options for schistosomiasis and is also applicable to diseases where strict adherence is important for efficacy.

[0290] Example 7 Ultrafast and stable coating of human tissue using tissue-accelerated polymerization The tissue-accelerated polymerization technique is applicable to human tissue and suitable for clinical translation. Freshly resected tissue specimens from human small intestine were coated to test their compatibility with the tissue-accelerated polymerization technique. Similar to the results of porcine tissue coating (Figure 1E), a polydopamine coating was clearly observed on the surface of human small intestine (Figure 5A). However, the polydopamine signal developed more rapidly in human tissue compared to porcine tissue, with the coating completion time decreasing from 12 to 3 minutes (Figure 5B), likely due to higher levels of catalase in the human small intestine. Analysis of the relationship between small intestinal catalase activity and tissue-accelerated polymerization coating density using human, porcine, and murine tissue specimens resulted in a clear correlation between tissue-accelerated polymerization coating density and enzyme activity levels (Figures 18A-18B). Human and porcine small intestines exhibited 60% more polydopamine development compared to mouse small intestine, likely due to their stronger enzyme activity. The similar tissue-accelerated polymerization efficiency (between humans and pigs) likely resulted from their genomic and proteomic similarities. To further demonstrate the robustness of the tissue-accelerated polymerization technique for human tissue coating, tissue specimens from three donors were tested with polydopamine coating. In addition, five evaluations were performed on random sites of the small intestine (from donors of different ages, races, and genders) to confirm consistent polydopamine coating performance. Additionally, it was confirmed that the human and pig GI tracts exhibited similar tissue-accelerated polymerization patterns (Figures 35A-35B), which is attributed to higher catalase expression in the small intestine compared to other segments of the GI tract in both species. 15As shown in Figure 5C, 30 images of tissue (6 mm diameter) revealed signal enhancement and a highly consistent polydopamine coating. These tissue specimens coated through the tissue-accelerated polymerization technique were further examined microscopically. Consistent with the porcine results (Figure 2G), microscopic and histological analysis of frozen formalin-fixed, paraffin-embedded (FFPE) specimens demonstrate a thin layer of polydopamine coating on the external villi of human small intestine (Figure 5D). After exposure to the tissue-accelerated polymerization solution, the epithelial layer remained intact, with a staining pattern similar to that of unexposed controls, demonstrating the absence of tissue toxicity.

[0291] To further evaluate the biocompatibility of the tissue-accelerated polymerization technology, we characterized the cytotoxicity of polydopamine against several cell lines: HeLa, COLO320DM, Caco-2, Hep3B, and HS 895.T (Figure 19). Results indicated that no significant cytotoxicity was observed after 6 to 48 hours of in vitro incubation. Furthermore, we systematically assessed the oral toxicity of the tissue-accelerated polymerization solution by following the guidelines published by the OECD with minor modifications. Therefore, rats were exposed to the tissue-accelerated polymerization solution for a 4-week period, based on a 28-day repeated-dose toxicity test method recommended by Good Laboratory Practice (GLP). No significant differences in body weight were observed between rats exposed to the tissue-accelerated polymerization solution and controls during the 28-day exposure period (Figures 20A-20C). Further hematological measurements, blood biochemistry tests (Figures 21 and 22), and histopathological tests further confirmed the lack of oral toxicity (Figure 23), supporting the desirable biocompatibility of the tissue-accelerated polymerization technology and implying that future preclinical and clinical trial risks are minimized.

[0292] The stability of the human tissue coating is a key factor for clinical translation of tissue-accelerated polymerization technology. The small intestine provides a dynamic environment in which physical forces (peristalsis and segmentation) and chemical exposures (chyme, gastric acid, and intestinal fluid) can damage the polydopamine coating (Figures 36A-36C). To assess the stability of the tissue coating, we tested it under a series of physical and chemical conditions. As shown in Figure 5E, no polydopamine signal reduction was observed under mechanical agitation and scratching. Quantitative signal intensity analysis showed that approximately 80% of the polydopamine remained strongly attached even after vigorous scratching of the small intestine using the back of a scalpel (Figure 5F). In addition, the stability of the polydopamine coating was further confirmed by incubating the polydopamine-coated human tissue in different solutions for 24 hours. As shown in Figure 5G, the polydopamine coating layer was highly stable in simulated intestinal and gastric fluids, as well as in other extreme conditions (ethanol and concentrated saline). These results confirmed the coating stability of the tissue-accelerated polymerization technology in the human small intestine and demonstrated the feasibility of broad applicability.

[0293] References [Table 1] [Table 2] [Table 3] [Table 4] [Table 5] [Table 6] Table 7 Table 8

Claims

1. A composition for use in a method for treating or preventing a disease in a subject by forming a polymer in situ in the subject, the method comprising administering to the subject a composition comprising a monomer and an oxygen source, wherein the oxygen source contacts a catalyst endogenous to the subject, and release of oxygen from the oxygen source polymerizes the monomer, wherein the monomer is dopamine or a salt thereof, the oxygen source is hydrogen peroxide or hydrogen peroxide urea, and the endogenous catalyst is selected from catalase or peroxidase.

2. 10. The composition of claim 1, wherein the composition further comprises an enzyme, a nutrient blocker, a dietary supplement, a radioprotectant, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof.

3. (a) the composition is administered orally, and / or (b) the composition comprises 0.001 to 1000 mg / mL of dopamine, and / or (c) the composition comprises 0.01 to 100 mM of an oxygen source; and / or (d) the composition has a pH of 7 to 10, and / or (e) the composition is in a liquid or solid dosage form, and / or (f) The composition of claim 1 or 2, wherein the composition is in the form of a solution, gel, tablet, or capsule.

4. (a) the polymer is formed in contact with the epithelium of the subject; and / or (b) the polymer adheres to the tissue of the subject; and / or (c) the polymer is formed on the epithelium of one or more of the subject's duodenum, jejunum, ileum, colon, esophagus, or stomach; and / or (d) the polymer is non-toxic; The composition according to any one of claims 1 to 3.

5. (a) the polymer and composition further comprise an enzyme; and / or (b) the polymer and composition further comprise a nutrient blocking agent; and / or (c) the composition further comprises a radioprotectant; The composition according to any one of claims 1 to 4.

6. The method comprises: (a) a method for improving digestive efficiency by a subject; and / or (b) a method for enhancing the digestion of lactose by a subject; and / or (c) a method for treating lactose intolerance in a subject; and / or (d) a method for preventing nutrient absorption in a subject; and / or (e) a method for regulating or controlling glucose absorption by a subject; and / or (f) a method for treating obesity in a subject; and / or (g) a method for treating hyperinsulinemia in a subject; and / or (h) a method of treating diabetes in a subject; The composition according to any one of claims 1 to 5.

7. the composition further comprises a crosslinker; The composition according to any one of claims 1 to 6.

8. the composition further comprises an active pharmaceutical ingredient; The composition according to any one of claims 1 to 7.

9. The method comprises: (a) a method of treating schistosomiasis in a subject, and / or (b) allowing targeting to the small intestine; and / or (c) a method for reducing uptake by the small intestine; and / or (d) a method for reducing the uptake of one or more nutrients and active pharmaceutical ingredients by the small intestine; and / or (e) increasing the residence time in the small intestine; and / or (f) increasing the residence time of one or more nutrients and / or active pharmaceutical ingredients in the small intestine; and / or (g) causing the intestinal lumen to remain enlarged; and / or (h) a method for treating or preventing intestinal adhesions in a subject; and / or (i) a method of treating or preventing ileus in a subject; and / or (j) a method for treating bleeding in the small intestine in a subject; and / or (k) the polymer modulates absorption in the small intestine, and / or (l) the polymer modulates digestion in the small intestine, and / or (m) the polymer modulates the absorption of one or more nutrients or active pharmaceutical ingredients or combinations thereof in the small intestine; and / or (n) the polymer substantially prevents absorption of one or more nutrients or active pharmaceutical ingredients, or a combination thereof, into the epithelium on which the polymer is formed; and / or (o) the polymer substantially prevents absorption of one or more nutrients or active pharmaceutical ingredients, or a combination thereof, into the bloodstream of the subject; and / or (p) a method for immobilizing an enzyme in a target; and / or (q) a method for delivering an active pharmaceutical ingredient to a subject; and / or (r) the method is a method of supplementing digestion in a subject; The composition according to any one of claims 1 to 8.

10. 1. A composition for administration to a subject comprising dopamine and an oxygen source, wherein the composition does not contain exogenous enzymes and the oxygen source is peroxide.

11. (a) the composition comprises 0.001 to 1000 mg / mL dopamine and 0.01 to 100 mM of an oxygen source; and / or (b) the composition comprises 0.001 to 1000 mg / mL of dopamine, and / or (c) the composition comprises 0.01 to 100 mM of an oxygen source; and / or (d) the composition comprises a source of oxygen at a concentration compatible with ingestion by the subject; and / or (e) the composition has a pH of 7 to 9, and / or (f) the composition comprises 10 mg / mL dopamine and 20 mM hydrogen peroxide or urea hydrogen peroxide; and / or (g) the composition further comprises a digestive enzyme, a nutrient blocker, a dietary supplement, a radioprotectant, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof; and / or (h) the composition is in a liquid or solid dosage form, and / or (i) the composition is in the form of a solution, gel, tablet or capsule; The composition of claim 10.

12. 12. The composition of claim 10 or 11 for use in treating or preventing a disease or disorder.

13. The disease or disorder is a metabolic disorder, a systemic disease, a digestive disorder, an infectious disease, a cancer, a bleeding disorder, an autoimmune disorder, or an inflammatory disorder; The composition according to any one of claims 1 to 9 and 12.

14. 14. The composition of any one of claims 1-9 and 12-13, wherein the composition is administered via an endoscope, arthroscope, cystoscope, colposcope, colonoscope, bronchoscope, ureteroscope, anoscope, esophagoscope, gastroscope, laparoscope, laryngoscope, neuroendoscope, rectoscope, sigmoidoscope, or thoracoscope.

15. A composition according to claim 10 or 11, and Instructions for administering the composition of claim 10 or 11. Includes a kit.

16. The polymer is formed in contact with the epithelium of the subject. The composition according to any one of claims 1 to 9, 13 and 14.

17. the polymer adheres to the intestinal epithelium of the subject; The composition according to any one of claims 1 to 9, 13, 14 and 16.

18. The polymer is formed on the duodenal epithelium of the subject. The composition according to any one of claims 1 to 9, 13, 14 and 16.

19. the composition further comprises a digestive enzyme; The composition according to any one of claims 1 to 9, 13, 14 and 16 to 18.

20. (i) the digestive enzyme is lactase, peptidase, sucrase, maltase, amylase, lipase, or protease, or (ii) the digestive enzyme is β-galactosidase; 20. The composition of claim 19.

21. the composition further comprises a nutrient blocker; The composition according to any one of claims 1 to 9, 13, 14 and 16 to 20.

22. wherein the method is a method of preventing nutrient absorption in a subject. The composition according to any one of claims 1 to 9, 13, 14 and 16 to 21.

23. The method is a method for regulating or controlling glucose absorption by a subject. The composition according to any one of claims 1 to 9, 13, 14 and 16 to 22.

24. The sugar is selected from glucose, lactose, fructose, maltose, dextrose, galactose, sucrose, and isomaltose; 24. The composition of claim 23.

25. the method is a method of treating obesity in a subject; The composition according to any one of claims 1 to 9, 13, 14 and 16 to 24.

26. the method is a method of treating hyperinsulinemia in a subject; The composition according to any one of claims 1 to 9, 13, 14 and 16 to 25.

27. the method is a method of treating diabetes or type 2 diabetes in a subject; The composition according to any one of claims 1 to 9, 13, 14 and 16 to 26.

28. the composition further comprises a cross-linking agent comprising nanoparticles; The composition of any one of claims 1 to 9, 13, 14, and 16 to 27.

29. the composition further comprises a cross-linking agent comprising polydopamine; The composition of any one of claims 1 to 9, 13, 14, and 16 to 27.

30. Active pharmaceutical ingredients include tumor necrosis factor, interferon alpha, interferon gamma, IL-1, IL-2, IL-4, IL-6, IL-12, GM-CSF, trastuzumab, T-DM1, bevacizumab, cetuximab, panitumumab, rituximab, tositumomab, tamoxifen, raloxifene, megestrol, goserelin, leuprolide, flutamide, bicalutamide, verteporfin (BPD-MA), phthalocyanine, photosensitizer Pc4, dextromethorphan, phenanthrene ... Methoxy-hypocrelin A (2BA-2-DMHA), cyclophosphamide, ifosfamide, trofosfamide, chlorambucil, estramustine, melphalan, carmustine (BCNU), lomustine (CCNU), busulfan, treosulfan, dacarbazine, temozolomide, cisplatin, carboplatin, oxaliplatin, vincristine, vinblastine, vindesine, vinorelbine, paclitaxel, nanoparticle albumin binding Conjugated paclitaxel, docosahexaenoic acid-bound paclitaxel (DHA-paclitaxel), polyglutamic acid-bound paclitaxel (PG-paclitaxel), paclitaxel poliglumex, CT-2103, ANG1005, paclitaxel-EC-1, 2'-paclitaxel methyl 2-glucopyranosyl succinate, docetaxel, taxol, etoposide, etoposide phosphate, teniposide, topotecan, 9-aminocamptothecin, camptothecin Ptoirinotecan, irinotecan, crisnatol, mitomycin C, methotrexate, dichloromethotrexate, trimetrexate, edatrexate, mycophenolate, tiazofurin, ribavirin, EICAR, hydroxyurea, deferoxamine, 5-fluorouracil (5-FU), floxuridine, doxifluridine, raltitrexed, tegafur uracil, capecitabine, cytarabine (ara) C), cytosine arabinoside, fludarabine, mercaptopurine, thioguanine, EB1089, CB1093, and KH1060, lovastatin, 1-methyl-4-phenylpyridinium ion, staurosporine, actinomycin D, dactinomycin, bleomycin, bleomycin A2, bleomycin B2, peplomycin, daunorubicin, doxorubicin, PEGylated liposomal doxorubicin,Idarubicin, epirubicin, pirarubicin, zorubicin, mitoxantrone, verapamil, thapsigargin, imatinib, thalidomide, lenalidomide, axitinib, bosutinib, cediranib, dasatinib, erlotinib, gefitinib, imatinib, lapatinib, lestaurtinib (CEP-701), neratinib (HKI-272), nilotinib, semaxanib, sunitinib, toceranib, vandetanib, vatalanib (PTK787, PTK / ZK), trastuzumab, bevacizumab, rituximab, cetuximab, panitumumab, ranibizumab nilotinib, sorafenib, everolimus, alemtuzumab, gemtuzumab ozogamicin, temsirolimus, ENMD-2076, PCI-32765, AC220, dovitinib lactate (TKI258, CHIR-258), BIBW2992 (TOVOK™), SGX523, PF-04217903, PF-02341066, PF-299804, BMS-777607, ABT-869, MP470, BIBF1120, AP24534, JNJ-26483327, MGCD265, DCC-2036, BMS-690154, CE P-11981, tivozanib (AV-951), OSI-930, MM-121, XL-184, XL-647, XL228, bortezomib, rapamycin, temsirolimus (CCI-779), everolimus (RAD-001), ridaforolimus, AP23573, AZD8055, BEZ235, BGT226, XL765, PF-4691502, GDC0980, SF1126, OSI-027, oblimersen, gemcitabine, carminomycin, leucovorin, pemetrexed, cyclophosphamide, dacarbazine, procarbazine, prednisolone Zolone, dexamethasone, campatecin, plicamycin, asparaginase, aminopterin, methopterin, porfiromycin, melphalan, leurocidin, leurosine, chlorambucil, trabectedin, procarbazine, discodermolide, carminomycin, aminopterin, hexamethylmelamine, penicillin, amoxicillin, cephalexin, erythromycin, clarithromycin, azithromycin, troleandomycin, ciprofloxacin, levofloxacin, ofloxacin, cotrimoxazole, trimethoprim,The composition of claim 8, which is tetracycline, chlortetracycline, oxytetracycline, demeclocycline, methacycline, sancycline, doxycycline, aureomycin, terramycin, minocycline, 6-deoxytetracycline, lymecycline, meclocycline, methacycline, rolitetracycline, tigecycline, gentamicin, tobramycin, paromomycin, spectinomycin, chloramphenicol, sparsomycin, quinupristin / dalfopristin, or praziquantel.

31. The polymer regulates absorption in the small intestine. The composition according to any one of claims 1 to 9, 13, 14 and 16 to 30.

32. The polymer regulates digestion in the small intestine. The composition according to any one of claims 1 to 9, 13, 14 and 16 to 31.

33. The polymer modulates the absorption of one or more nutrients in the small intestine. The composition according to any one of claims 1 to 9, 13, 14 and 16 to 32.

34. The polymer modulates the absorption of one or more active pharmaceutical ingredients in the small intestine. The composition according to any one of claims 1 to 9, 13, 14 and 16 to 33.

35. The composition of any one of claims 1 to 14 and 16 to 34, further comprising a buffering agent.

36. the disease or disorder is an ulcer, intestinal obstruction, mesenteric ischemia, obesity, Alzheimer's disease, transplant rejection, hyperinsulinemia, Crohn's disease, ulcerative colitis, malabsorption, inflammatory bowel disease, irritable bowel syndrome, lactose intolerance, celiac disease, mastocytosis, chronic fatigue syndrome, systemic vasculitis, sarcoidosis, hypothyroidism, diabetes, fibromyalgia, adrenal insufficiency, hypertension, metabolic syndrome, AIDS, Graves' disease, systemic lupus erythematosus, arthritis, atherosclerosis, sickle cell disease, myasthenia gravis, systemic sclerosis, sinusitis, bacterial infection, viral infection, fungal infection, parasitic infection, parasitic disease, giardiasis, ascariasis, tapeworm infection, or schistosomiasis; The composition according to any one of claims 1 to 9, 12 to 14 and 16 to 35.

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