Oral composition

Abstract

To provide an oral composition containing terminalia, in which the bitterness and astringency is improved.SOLUTION: Provided is an oral composition containing a terminalia, and other materials.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an oral composition characterized by containing Terminalia and at least one other material selected from plant materials and functional materials, and having an improved taste of Terminalia.

Background Art

[0002] Terminalia is a plant of the Combretaceae family and has been used as a medicine in India. In recent years, an effect of preventing vascular fibrosis has been found in Terminalia, and it is expected to be a preventive or therapeutic agent for vascular fibrosis (for example, see Patent Document 1).

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] However, despite the recognized effectiveness as described above, Terminalia has a unique bitterness and astringency, and therefore, improvement in palatability is required for continuous intake as a health food.

[0005] The present invention has been made for the purpose of improving bitterness and astringency in a food composition containing Terminalia.

Means for Solving the Problems

[0006] As a result of intensive studies to solve the above problems, the present inventors have found that the taste is improved by combining Terminalia with a specific material, and thus have arrived at the present invention.

[0007] Furthermore, the inventors discovered that combining Terminalia with specific materials exhibits excellent anti-obesity effects, leading to the present invention.

[0008] In other words, the present invention is as follows: [1] An oral composition characterized by containing terminalia and at least one other material selected from (a) and (b) below. (a) At least one plant material selected from Cimicifuga simplex, white bean, kale, maca, cacao, acerola, mangosteen, coconut, dragon fruit, camu camu, and prune. (b) At least one functional material selected from red yeast rice, royal jelly, ceramide, and spirulina. [2] An oral composition characterized by containing terminalia and at least two other materials selected from (a) and (b) below. (a) At least one plant material selected from Cimicifuga simplex, white bean, kale, maca, cacao, acerola, mangosteen, coconut, dragon fruit, camu camu, and prune. (b) At least one functional material selected from red yeast rice, royal jelly, ceramide, and spirulina. [3] An oral anti-obesity composition characterized by containing terminalia and at least one other material selected from (a) and (b) below. (a) At least one plant material selected from Cimicifuga simplex, white bean, kale, maca, cacao, acerola, mangosteen, coconut, dragon fruit, camu camu, and prune. (b) At least one functional material selected from red yeast rice, royal jelly, ceramide, and spirulina. [4] An oral anti-obesity composition characterized by containing terminalia and at least two other materials selected from (a) and (b) below. (a) At least one plant material selected from Cimicifuga simplex, white bean, kale, maca, cacao, acerola, mangosteen, coconut, dragon fruit, camu camu, and prune. (b) At least one functional material selected from red yeast rice, royal jelly, ceramide, and spirulina. [5] The oral composition according to [1] to [4], characterized by containing 0.2% or more of other materials per 1% of terminalia. [6] An oral composition according to [1] to [5], characterized by containing 0.1% or more of gallic acid or a salt thereof. [Effects of the Invention]

[0009] The present invention provides an oral composition containing Terminalia that, by combining specific materials, can improve bitterness, astringency, aroma, astringency, mildness, richness, flavor intensity, texture, and aftertaste, thereby providing a composition with improved taste. Furthermore, the present invention can provide an oral composition with excellent anti-obesity activity. [Brief explanation of the drawing]

[0010] [Figure 1] This figure shows the sensory evaluation results for Examples 1-18 and Comparative Examples 1-2. [Figure 2] This figure shows the evaluation results (amount of lipid droplets per cell) of the inhibitory effect on adipocyte differentiation in Examples 19-23 and Comparative Examples 3-8. [Modes for carrying out the invention]

[0011] The composition of the present invention is characterized by containing terminalia and at least one material selected from the group consisting of (a) and (b) below (hereinafter sometimes referred to as "other material"). The materials (a) and (b) used together with terminalia may be used individually or in combination of two or more. When two or more other materials are used in combination, it is preferable to combine other materials that have a high effect when combined with terminalia.

[0012] The composition of the present invention suppresses the differentiation of adipocytes, thereby suppressing obesity. That is, the oral composition of the present invention can be used for anti-obesity, reduction of body fat, dieting, and the like.

[0013] The anti-obesity effect in the present invention refers to an effect of suppressing the accumulation of body fat, an effect of reducing body fat, an effect of suppressing the accumulation of visceral fat, an effect of reducing visceral fat, an effect of suppressing the accumulation of subcutaneous fat, an effect of reducing subcutaneous fat, an effect of suppressing weight gain, an effect of reducing weight, or an effect of suppressing the differentiation of preadipocytes into adipocytes.

[0014] [Terminalia] Terminalia is a broad-leaved tree of the genus Terminalia in the Combretaceae family. Examples of Terminalia that can be used in the present invention include Terminalia bellirica (belerica), Terminalia catappa, Terminalia tomentosa, Terminalia citrina, Terminalia phellocarpa, Terminalia copelandii, Terminalia brassi, Terminalia ivorensis, Terminalia superba, Terminalia arjuna, Terminalia chebula, and the like. Among these, Terminalia bellirica (belerica) and Terminalia chebula are preferred, and Terminalia bellirica (belerica) is particularly preferred from the viewpoint of excellent anti-obesity effect.

[0015] The parts of Terminalia used in the present invention include leaves, bark, roots, flowers, xylem, fruits, seeds, etc. From the viewpoint of the anti-obesity effect, it is preferable to use parts excluding fruits or seeds of fruits (peel or pulp part). Also, as Terminalia, it is preferable to use a processed Terminalia product.

[0016] The processed forms of Terminalia processed products include dried products, crushed products or their dried powders (hereinafter also referred to as "crushed powders"), squeezed juices or their dried powders (hereinafter also referred to as "squeezed powders"), extracts or their dried powders (hereinafter also referred to as "extract powders"), granules, pastes, etc. In the present invention, crushed products or crushed powders, squeezed juices or squeezed powders, extracts or extract powders are preferred, and it is more preferred to use extracts or extract powders. In the present invention, from the viewpoint of the anti-obesity effect, it is most preferred to use the water extract or extract powder of fruits.

[0017] Examples of the solvent used for extraction include water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, ethyl acetate, ether, hexane, or a mixed solvent thereof. From the viewpoint of the anti-obesity effect, it is preferable to use water. The temperature of the extraction solvent can be appropriately set at room temperature to below the boiling point according to the solvent used.

[0018] The Terminalia of the present invention preferably contains gallic acid. The amount of gallic acid contained in Terminalia is preferably 0.01% or more, more preferably 0.05% or more, and even more preferably 0.1% or more from the viewpoint of the anti-obesity effect. In the present invention, commercially available products can be used.

[0019] [Other materials] In the present invention, together with Terminalia, it contains at least one other material selected from (a) plant materials and (b) functional materials. Hereinafter, the other materials will be described in detail.

[0020] (a) Plant materials The plant materials used in the composition of the present invention are at least one selected from acacia, white bean, kale, maca, cacao, acerola, mangosteen, coconut, dragon fruit, camu camu and prune. Examples of the parts that can be used for plant materials include whole plants, flowers, seeds, fruits, leaves, stems and roots, etc. As the plant material, it is preferable to use the processed product of the plant material.

[0021] The processed forms of plant materials include dried plants, pulverized plants or powdered plants, juices or powdered juices, extracts or powdered extracts, granules, and pastes. Extracts can be obtained by extraction using a suitable solvent, such as water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, ethyl acetate, ether, hexane, or mixtures thereof. The temperature of the extraction solvent can be appropriately set between room temperature and below the boiling point, depending on the solvent used. Commercially available plant materials may also be used.

[0022] (Astilbe thunbergii) Astilbe thunbergi is a perennial herbaceous plant belonging to the genus Astilbe in the family Saxifragaceae, and its scientific name is Astilbe thunbergi. The roots of Astilbe thunbergi contain health-promoting components such as bergenin and Astilbe polyphenols. In this invention, the roots are preferred as the part of Astilbe thunbergi used. In this invention, various processed products of Astilbe thunbergi can be used, and the preferred processed forms are pulverized or pulverized powder, juice or juice powder, extract or extract powder, extract or extract powder, more preferably extract or extract powder, and particularly preferably aqueous ethanol extract or extract powder. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, it is most preferable to use aqueous ethanol extract or extract powder of the roots.

[0023] (White beans) White beans are an annual plant belonging to the genus Phaseolus, subfamily Fabaceae, and their scientific name is Phaseolus vulgaris. White bean seeds contain components such as phaseolin. In this invention, the seeds are preferred as the part of the white bean used. In this invention, various processed products of white beans can be used, and the preferred processing forms are pulverized or pulverized powder, juice or juice powder, extract or extract powder, extract or extract powder, more preferably extract or extract powder, and particularly preferably aqueous extract or extract powder. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, the aqueous extract or extract powder of the seeds is most preferred.

[0024] (Kale) Kale is a plant belonging to the Brassicaceae family, and its scientific name is Brassica oleracea var. acephala. There are no particular restrictions on the variety of kale used, and various types of kale such as kitchen kale, tree kale, bush kale, mallow kale, collard kale, and green leaf kale can be used. In this invention, the stems and leaves are preferred as the part of the kale used. In addition, various processed products of kale can be used in this invention, and the preferred processing forms are crushed or ground powder, juice or ground juice, extract or extracted powder, with crushed or ground powder being more preferred. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, it is most preferable to use crushed or ground powder of the stems and leaves.

[0025] (Maca) Maca is a plant belonging to the genus Lepidium in the family Brassicaceae, and its scientific name is Lepidium peruvianum Chacon. For example, in addition to minerals and amino acids, maca contains nitrogen-containing compounds, such as alkaloids. In this invention, the root is preferred as the part of the maca used. Furthermore, in this invention, various processed maca products can be used, and the preferred processed forms are pulverized or powdered, juice or powdered juice, extract or powdered extract, with pulverized or powdered being more preferred. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, it is most preferable to use pulverized or powdered root.

[0026] (Cacao) Cacao is a fruit encased in a hard, rugby-ball-shaped shell called a cacao pod, and its scientific name is Theobroma cacao. Generally, the cacao seed, the cacao bean, is used. Cacao beans are typically fermented and dried for 3-5 days before undergoing various processing steps. For example, cacao beans are roasted to remove the skin and germ, then ground into cacao mass, which is then mixed with sugar, milk powder, and cocoa butter to produce chocolate. Alternatively, the cacao mass can be processed to produce cocoa powder by removing most of the fat. In this invention, the cacao seed is preferred. Furthermore, various processed cacao products can be used, with preferred processing forms including pulverized or ground powder, juice or ground juice, and extract or extracted powder, with pulverized or ground powder being more preferred. In this invention, from the viewpoint of improving taste (bitterness, astringency, etc.) and anti-obesity effects, it is most preferable to use pulverized or ground cocoa from which oils and fats have been removed from the seeds.

[0027] (Acerola) Acerola is a fruit tree belonging to the Malpighiaceae family that inhabits tropical regions, and its scientific name is Malpighia emarginata. The fruit of the acerola is rich in vitamin C and polyphenols. In this invention, the fruit is preferred as the part of the acerola to be used. In addition, in this invention, various processed products of acerola can be used, and the preferred processed forms are crushed or ground powder, juice or juice powder, extract or extract powder, and the use of juice or juice powder is more preferred. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, the use of fruit juice or juice powder is most preferred.

[0028] (mangosteen) Mangosteen is an evergreen tree belonging to the genus Garcinia, with the scientific name Garcinia mangostana L. The fruit of the mangosteen is rich in nutrients such as macululin glycosides, polyphenols, and xanthones. In this invention, the fruit is preferred as the part of the mangosteen to be used. In this invention, various processed mangosteen products can be used, and the preferred processing forms are pulverized or pulverized powder, juice or juice powder, extract or extract powder, with the use of extract or extract powder being more preferred, and water extract or extract powder being particularly preferred. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, the use of water extract or extract powder of the fruit is most preferred.

[0029] (Coconut palm) The "coconut palm" used in this invention refers to a plant of the Arecaceae family, genus Cocos (Cocos L.), with the scientific name Cocos nucifera L. Preferably, coconut palms or other plants commonly called coconuts can be used. In this invention, the seeds are preferred as the part of the coconut palm to be used. Furthermore, various processed coconut palm products can be used in this invention, and preferred processing forms include crushed or ground powder, juice or juice powder, extract or extract powder. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, it is most preferable to use the endosperm of the seed. In addition, commercially available coconut milk can also be used.

[0030] (Dragon fruit) Dragon fruit is the fruit of a tropical evergreen perennial plant belonging to the genus Hylocereus in the family Cactaceae, and its scientific name is Hylocereus undatus. In this invention, the fruit is preferred as the part of the dragon fruit used. In this invention, various processed products of dragon fruit can be used, and the preferred processing forms are crushed or ground powder, juice or juice powder, extract or extract powder, with the use of juice or juice powder being more preferable. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, the use of fruit juice or juice powder is most preferable.

[0031] (Come come) Camu camu is a berry-type plant belonging to the Myrtaceae family, native to the tropical rainforests of South America, and its scientific name is Myrciaria dubia. In this invention, the fruit is preferred as the part of the camu camu used. In addition, various processed products of camu camu can be used in this invention, and preferred processing forms are pulverized or powdered, juice or powdered juice, extract or powdered extract, with the use of juice or powdered juice being more preferable. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, it is most preferable to use the juice or powdered juice from the pericarp and pulp, which are the parts of the fruit excluding the seeds.

[0032] (prune) Prunes are plants belonging to the genus Prunus in the family Rosaceae, and their scientific name is Prunus domestica. In this invention, the fruit is preferred as the part of the prune to be used. In addition, various processed products of prunes can be used in this invention, with the fruit being preferred as the part to be used, and the processed form being a crushed product or crushed powder, juice or juice powder, or extract or extracted powder, with the use of an extract or extracted powder being more preferred. In this invention, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, the use of a fruit extract or extracted powder is most preferred.

[0033] (b) Functional materials The functional material used in the composition of the present invention is at least one selected from red yeast rice, royal jelly, ceramide, and spirulina. It is preferable to use a processed product of the functional material.

[0034] The processed forms of functional materials include dried materials, pulverized materials or powdered materials, extracts or extracted powders, granules, and pastes. Extracts can be obtained by extraction using a suitable solvent, such as water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, ethyl acetate, ether, hexane, or mixtures thereof. The temperature of the extraction solvent can be appropriately set between room temperature and below the boiling point, depending on the solvent used. Commercially available plant materials may also be used.

[0035] (red yeast rice) Any Monascus species belonging to the genus Monascus can be used, such as Monascus pilosus, Monascus anka, or Monascus purpureus. Monascus is rich in monacolin K. The fungal cells may be live or dead. In this invention, various processed products of Monascus can be used, but from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, it is preferable to use Monascus pilosus, and it is particularly preferable to use a dried powder of dead cells.

[0036] (Royal jelly) Royal jelly is a secretion produced by young worker bees that consume pollen and honey, break them down and synthesize them in their bodies, and secrete them from the pharyngeal glands and mandibular glands of the upper and lower jaws. It is ingested by larvae that will become queen bees and by adult bees. In this invention, various processed royal jelly products can be used, but from the viewpoint of improving taste, such as bitterness and astringency, and anti-obesity effects, it is preferable to use dried powder.

[0037] (Ceramide) Ceramides are a type of sphingolipid and are a general term for a group of compounds in which sphingosine and fatty acids are linked by an amide bond. In the oral composition of the present invention, the ceramide can be human-type ceramides such as ceramide 1, ceramide 2, and ceramide 3, as well as animal-derived ceramides extracted from the brains and spinal cords of animals such as cattle, horses, and pigs, and plant-derived ceramides extracted from wheat, rice, soybeans, spinach, corn, konjac, pineapple, etc. In addition, the ceramide in the oral composition of the present invention may be a glycoceramide, specifically, for example, those to which monosaccharides such as galactosylceramide and glucosylceramide are bonded, or those to which oligosaccharides are bonded. In the present invention, it is preferable to use plant-derived ceramides, and it is even more preferable to use glucosylceramides derived from grasses such as wheat, corn, and rice. Among these, it is particularly preferable to use glucosylceramide extracted from rice or seeds collected from rice, from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects. Ethanol is preferred as the extraction solvent from the viewpoint of improving taste, such as bitterness and astringency, and anti-obesity effects.

[0038] (Spirulina) Spirulina is a type of cyanobacteria belonging to the genus Spirulina, and is rich in chlorophyll, carotene, B vitamins, potassium, magnesium, and other minerals. Examples of spirulina that can be used in this invention include Spirulina gaitrelli, Spirulina pratensis, Spirulina maxima, Spirulina meyer, Spirulina lacissima, and Spirulina aldaria. Various processed spirulina products can be used in this invention, but it is preferable to use live bacteria from the viewpoint of improving taste such as bitterness and astringency and anti-obesity effects, and it is particularly preferable to use pulverized or powdered live bacteria.

[0039] The oral composition of the present invention can be used, for example, as a pharmaceutical product (including quasi-drugs), a functional food such as a food for specified health uses, a food with nutritional function claims, or a food with functional claims whose efficacy has been approved by a designated institution, as well as a general food, food additive, animal feed, etc.

[0040] The oral composition of the present invention can be used as an anti-obesity composition having an anti-obesity effect. Such an anti-obesity composition is not particularly limited as long as it can be distinguished from other products as a product in that it is used for anti-obesity (obesity suppression). For example, the scope of the present invention includes not only products that display on the product body, packaging, instructions, or promotional materials that they have functions such as anti-obesity, diet, body fat reduction, improvement of metabolic syndrome, and weight loss, but also products that display other functions, or even products that do not display any functions but substantially have an anti-obesity effect. Furthermore, the anti-obesity composition of the present invention is not limited to products in which the combination of components of the present invention (Terminalia and other materials) is displayed as an active anti-obesity ingredient on the product packaging, etc. For example, the active ingredient may not be specified, or only Terminalia may be displayed as the active ingredient.

[0041] Since the oral composition of the present invention contains terminalia having gallic acid or a salt thereof, which has an effect of suppressing the rise in triglycerides after meals, it can also be used as a composition for suppressing the rise in triglycerides. Such a composition for suppressing the rise in triglycerides is not particularly limited as long as it can be distinguished from other products in that it is used to suppress the rise in triglycerides. For example, the scope of the present invention includes not only products that indicate on the product body, packaging, instructions, or promotional materials that it has functions such as suppressing the rise in triglycerides after meals or lowering triglycerides after meals, but also products that indicate other functions, or even products that do not indicate functions but substantially have an effect of suppressing the rise in triglycerides. Furthermore, the composition for suppressing the rise in triglycerides of the present invention is not limited to products in which the combination of components of the present invention (terminalia and other materials) is indicated as the active ingredient for suppressing the rise in triglycerides on the product packaging, etc. For example, it may not specify an active ingredient, or it may only indicate terminalia as the active ingredient.

[0042] Specifically, examples of the anti-obesity composition and the triglyceride elevation suppression composition of the present invention include pharmaceuticals (including quasi-drugs) and so-called health foods. Examples of health foods that promote anti-obesity include those that display phrases such as "For those concerned about body fat," "For those who are overweight," "For those concerned about their weight (BMI)," "Reduce weight and abdominal fat (visceral fat and subcutaneous fat)," and "Reduce waist circumference." Examples of health foods that promote triglyceride elevation suppression include those that display phrases such as "For those concerned about triglycerides," "Suppress the rise in triglycerides," "Lower triglycerides," "Slow down the rise in triglycerides," and "Suppress the absorption of ingested fat."

[0043] Examples of the composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, chewables, sticks, and the like. Among these, tablets, capsules, powders, granules, and liquids are preferred, with tablets, powders, and granules being particularly preferred. Specifically, examples include supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant beverages to be dissolved in water (hot water), milk, fruit juice, green juice, etc., and food additives. Supplements and instant beverages are particularly preferred because they are easy to consume with meals and can enhance palatability.

[0044] A composition according to one aspect of the present invention can be a packaged composition sealed in a container. The container is not particularly limited, but examples include packaging containers made of metal such as aluminum, paper, plastics such as PET or PTP, single-layer or laminated film bags, retort pouches, vacuum packs, aluminum containers, plastic containers, bottles, cans, etc. In order to avoid deterioration over time, it is preferable that a composition according to one aspect of the present invention is sterilized by pressurization and / or heating after being sealed in a container.

[0045] The method of use of the composition according to one aspect of the present invention is not particularly limited, but for example, the composition according to one aspect of the present invention can be taken orally by eating or drinking, either as is, with water or other liquids, or diluted with water or other liquids. Depending on the preference of the person taking the product, the composition according to one aspect of the present invention may be mixed with other solid or liquid substances and taken orally. If the composition according to one aspect of the present invention is in an orally disintegrating form, it can be taken orally without water.

[0046] The content of Terminalia and other materials (components of the present invention) in the composition of the present invention may be appropriately included within a range that achieves the desired effect.

[0047] Generally, when the oral composition of the present invention is in the form of a tablet, chewable tablet, or other supplement or pharmaceutical product, it is preferable that the components of the present invention make up 1-80% of the total on a dry weight basis, more preferably 5-60%, and even more preferably 10-50%.

[0048] When the oral composition of the present invention is a packaged beverage, it is preferable that the components of the present invention are present in an amount of 0.001 to 5% of the total, more preferably 0.005 to 3%, and even more preferably 0.01 to 1%.

[0049] Furthermore, when the oral composition of the present invention is an instant beverage, it is preferable that the components of the present invention make up 0.1 to 50% of the total on a dry weight basis, more preferably 0.5 to 30%, and even more preferably 1 to 20%.

[0050] In order to more effectively exhibit the effects of the present invention, it is preferable that the components of the present invention make up 70% or more of the total composition of the present invention on a dry weight basis, more preferably 80% or more, even more preferably 90% or more, and particularly preferably 100%.

[0051] There are no particular restrictions on the amount of the oral composition of the present invention to be taken, but from the viewpoint of more significantly exhibiting the effects of the present invention, it is preferable for adults to take at least 10 mg / day of Terminalia per day, more preferably 50 mg / day or more, and even more preferably 100 mg / day or more. The upper limit is, for example, 10,000 mg / day, and preferably 5,000 mg / day. The oral composition of the present invention can be contained in one container, or divided into, for example, two or three containers, to constitute one day's dose, so that the daily intake amount is as described above.

[0052] The oral composition of the present invention preferably contains gallic acid or a salt thereof. There are no particular restrictions on the gallic acid or salt thereof, including those obtained by synthesis, those extracted from natural products, and those obtained by treating extracts with enzymes, etc., but from the viewpoint of anti-obesity effects, it is preferable to contain gallic acid or a salt thereof derived from Terminalia. The content of gallic acid or a salt thereof in the present invention is preferably 0.001% or more, more preferably 0.01% or more, and particularly preferably 0.1% in the composition. Furthermore, the content of gallic acid or a salt thereof is preferably 30% or less, more preferably 20% or less, and particularly preferably 10% or less in the composition.

[0053] The blending mass ratio of Terminalia and other materials is preferably in the range of 0.25 to 100,000:1 on a dry weight basis, more preferably in the range of 0.5 to 75,000:1, even more preferably in the range of 0.75 to 50,000:1, and particularly preferably in the range of 1 to 30,000:1.

[0054] The compositions of the present invention can be manufactured by known methods, with the addition of other components besides the components of the present invention as needed. Examples of other components besides the components of the present invention include water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P), oil-soluble vitamins (vitamins A, D, E, K), etc.; minerals such as calcium, magnesium, phosphorus, and iron; sulfur-containing compounds found in taurine, garlic, etc.; flavanoids such as hesperidin and quercetin; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; and crushed or extracted animal and plant materials. [Examples]

[0055] The present invention will be described in detail below with reference to examples. However, the present invention is not limited to these examples.

[0056] [Sensory evaluation of food compositions] 1. Manufacturing of food and beverage compositions Food compositions having the formulations shown in Table 1 below were prepared. In Table 1, the numerical values ​​represent mass (g).

[0057] The following substances were used as test materials. For the terminalia, we used a commercially available aqueous extract of the fruit of Terminalia bellirica. For the Astilbe japonica extract, we used a commercially available hydrated ethanol extract powder of the Astilbe japonica root. For the white beans, we used a commercially available water extract powder of white bean seeds. For the kale, we used crushed kale leaf powder (manufactured by Toyo Shinyaku Co., Ltd.). For the maca, we used a commercially available powder made from crushed maca bulbs. For the cacao, I used commercially available cocoa powder. For the acerola, we used commercially available acerola fruit juice powder. For the mangosteen, we used a commercially available water extract powder of mangosteen fruit. For the coconut, I used commercially available coconut milk. For the dragon fruit, commercially available dragon fruit juice powder was used. For the camu camu, we used commercially available camu camu fruit juice powder. For the prune component, we used commercially available prune fruit extract powder. For the red yeast rice, we used a commercially available dried powder of dead Monascus pilosus bacteria. For the royal jelly, we used commercially available dried royal jelly powder. As the ceramide, we used a commercially available aqueous ethanol extract powder of rice seeds containing 10% or more glucosylceramide. For the spirulina, I used a commercially available powdered live spirulina. For the guava leaves used, we used a commercially available hydrated ethanol extract powder of guava leaves.

[0058] [Table 1]

[0059] 2. Sensory evaluation (1) Sample preparation For the samples of Comparative Examples 1-2 and Examples 1-18 listed in Table 1 above, the specified mass was mixed with 100 mL of water to obtain each test sample.

[0060] (2) Evaluation of the sample Six healthy adults were randomly selected as subjects. These six subjects were given a questionnaire and sensory evaluation based on the evaluation items in Table 2 below. Specifically, for example, the evaluation of Examples 1-18 and Comparative Example 2 was based on Comparative Example 1 as the baseline (5 points), and each sample was scored from 1 to 10 points.

[0061] [Table 2]

[0062] For each sample, the average score of the subjects was calculated. The cumulative average value of each calculated evaluation item is shown in the graph in Figure 1, illustrating the difference compared to the baseline comparative example 1.

[0063] (3) Discussion We examined the results of the palatability evaluation for each comparative example and example.

[0064] As shown in Figure 1, Comparative Example 2, which combined terminalia with guava leaves, showed higher values ​​in all categories compared to Comparative Example 1, which contained terminalia alone. Furthermore, Examples 1 to 15, which combined terminalia with one other material selected from (a) and (b), showed improvement compared to Comparative Examples 1 and 2. In particular, they received high marks for smoothness, bitterness, and astringency while drinking, indicating that compositions with improved taste could be obtained.

[0065] Furthermore, Examples 16-18, which combined Terminalia with two other materials selected from (a) and (b), were superior in all aspects compared to the compositions of Examples 1-15. In particular, they received high marks for smoothness, bitterness, and astringency while drinking, indicating that compositions with excellent palatability could be obtained. It was found that by combining these three components, compositions with even better taste could be obtained.

[0066] From the above results, the composition using terminalia alone (Composition I) had a strong bitterness and astringency characteristic of terminalia, and was not palatable on its own. The composition of the present invention containing terminalia and one other material selected from (a) and (b) (Composition II) showed improvement in the strong bitterness and astringency of terminalia compared to Composition I, and was given a milder taste. Furthermore, the composition of the present invention containing terminalia and two other materials selected from (a) and (b) (Composition III) was found to be able to effectively improve the strong bitterness and astringency of terminalia compared to Compositions I and II, resulting in a composition with an improved taste and a milder flavor.

[0067] [Evaluation of the anti-obesity effects of food compositions] Test name: Evaluation of the inhibitory effect of fibroblasts on adipogenic differentiation

[0068] (1) Cell culture (method for inducing differentiation into adipocytes) 75cm in a 37℃, 5% CO2 incubator. 2 Mouse fibroblast cell line (3T3-L1) was cultured in a flask using DMEM containing 10% (V / V) FBS. Cells suspended by trypsin were then cultured at 75 cm³. 2 Each well of a collagen-coated 96-well plate, with a cell count of 1 × 10⁶ cells per well, was transferred from a flask. 4 Cells were seeded at the specified cell density. They were cultured in a 37°C, 5% CO2 incubator until confluence was reached. Three days after confluence, a sample containing the test substance, prepared in differentiation induction medium, was added and cultured for nine days. During this time, the sample containing the test substance was replaced every two to three days with a sample containing the test substance, prepared in differentiation maintenance medium. Cells that did not undergo differentiation induction were cultured in 10% (V / V) FBS-containing DMEM with the same concentration of dimethyl sulfoxide as the sample containing the test substance.

[0069] The differentiation induction medium was prepared by adding 0.5 mM 3-isobutyl-1-methylxanthine, 0.5 μM dexamethasone, and 10 μg / mL insulin to 10% (V / V) FBS-containing DMEM. The differentiation maintenance medium was prepared by adding 10% (V / V) FBS-containing DMEM with 10 μg / mL human-derived insulin.

[0070] The following substances were used as test materials. For the terminalia, we used a commercially available aqueous extract of the fruit of Terminalia bellirica. For the purpose of identifying the Astilbe japonica plant, we used a commercially available aqueous ethanol extract powder of the roots of Astilbe japonica. For the maca component, we used a water-containing ethanol extract powder of maca bulb (commercially available). For the cacao, we used a commercially available aqueous ethanol extract powder of cacao seeds. For the camu camu, we used dried powder of camu camu fruit juice (commercially available). For the red yeast rice, we used a commercially available dried powder of dead Monascus pilosus bacteria. As ceramide, we used ethanol extract powder of rice seeds (commercially available).

[0071] For the samples in Comparative Examples 3-8 and Examples 19-23 listed in Table 3 below, test samples were obtained to achieve the concentrations indicated. The concentration unit is μg / mL. [Table 3]

[0072] (2) Staining of intracellular lipid droplets After culturing, 100 μL of the test substance and 100 μL of 10% formalin solution were added to the cells, and the mixture was left to stand at room temperature for 10 minutes in the dark. The formalin solution was removed, and 100 μL of fresh 10% formalin solution was added to each well. The mixture was left to stand at room temperature for 10 minutes in the dark to fix the cells. The formalin solution was removed, and the mixture was washed twice with phosphate buffer (PBS). 50 μL of 60% isopropanol-oil red solution was added to the cell-seeded wells and blank wells, and the mixture was left to stand at room temperature for 30 minutes in the dark to stain the lipid droplets. The staining solution was removed, and 150 μL of 60% isopropanol was added to each well. The 60% isopropanol was removed, and the mixture was washed once with PBS. 50 μL of 100% isopropanol was added to the cell-seeded wells and blank wells, and the mixture was shaken for about 10 minutes to extract the staining solution. The absorbance of the isopropanol solution from which the staining solution was extracted was measured at 520 and 650 nm (turbidity). After completely removing the isopropanol from the plate, the cells were disrupted in PBS, and the amount of protein per well was measured using a protein assay BCA kit (thermo). (3) Data analysis Based on the obtained data, the "% of control" (relative value to the control) was calculated using the following formula.

[0073] Data = [(Sample 520nm - blank 520nm) - (Sample 650nm - blank 650nm) / [Amount of protein per well] % of control=[SampleDate] / [ControlDate]×100 Sample520nm and blank520nm represent the absorbance values ​​at 520nm after oil red staining, while Sample650nm and blank650nm represent the absorbance values ​​at 650nm (turbidity) after oil red staining.

[0074] Figure 2 shows the evaluation results for the amount of lipids in mouse fibroblasts. Figure 2 shows the decrease in intracellular lipid droplets compared to the control group. A higher value indicates suppression of adipocyte differentiation. The concentration units of the added components are μg / mL.

[0075] As shown in Figure 2, the compositions of the present invention using Terminalia and other materials dramatically suppressed adipocyte differentiation compared to each material used alone. Specifically, in Examples 20 to 23, which combined Terminalia with the other materials from Comparative Examples 5 to 8, a high level of effect was observed that could not be predicted from the effects of each material alone. Furthermore, in Example 19, which combined Terminalia with the other material from Comparative Example 4, a remarkable effect was observed that could not be obtained by simply adding up the effects of each material.

[0076] Since obesity is caused by the enlargement and proliferation of adipocytes, it is thought that obesity can be prevented by suppressing the differentiation of adipocytes. Therefore, the oral composition of the present invention is useful for preventing obesity.

[0077] [Production of the composition of the present invention] [Example 24] (Manufacturing of tablets) Tablets (250 mg) consisting of the following ingredients were manufactured. Five of these tablets should be taken daily. The manufactured tablets have an improved taste characteristic of Terminalia (bitterness, astringency, etc.), and excellent anti-obesity effects can be obtained by taking them once a day or in two or three divided doses with water.

[0078] Terminalia bellirica fruit water extract powder 25% 25% aqueous ethanol extract powder of Astilbe thunbergii 1% aqueous ethanol extract powder from maca bulbs 5% aqueous ethanol extract powder of cocoa seeds Calcium stearate 2% Silicon dioxide 2% Reduced maltose remainder

[0079] [Example 25] (Manufacturing of tablets) Tablets (350 mg) consisting of the following ingredients were manufactured. Six of these tablets should be taken daily. The manufactured tablets have an improved taste characteristic of Terminalia (bitterness, astringency, etc.), and excellent anti-obesity effects can be obtained by taking them once a day or in two or three divided doses with water.

[0080] 15% crushed Terminalia bellirica fruit powder White kidney bean water extract powder 30% Rice-derived glucosylceramide 0.1% Pullulan 1% Reduced maltose remainder

[0081] [Example 26] (Manufacturing of capsules) The following mixture was enclosed in hard capsules to produce capsules (300 mg). Five of these hard capsules are taken daily. Taking the prepared capsules with water once a day or in 2-4 divided doses provides excellent anti-obesity effects.

[0082] Terminalia bellirica fruit hydrated ethanol extract powder 50% Royal jelly dried powder 0.5% Kotsusaiho aqueous ethanol extract powder 0.5% Spirulina dried powder 0.5% Mangosteen fruit extract powder 0.5% Lactic acid bacteria 5% Silicon dioxide 2% Cellulose residue

[0083] [Example 27] (Manufacture of granular formulation) A granular preparation (3000 mg) was manufactured by mixing the following ingredients using a conventional method. The resulting granular preparation had an improved taste characteristic of Terminalia (bitterness, astringency, etc.), and when taken with water, it provided excellent anti-obesity effects. In addition, the resulting granular preparation contained 0.1% gallic acid or its salt.

[0084] Terminalia bellirica fruit hot water extract powder 30% 10% aqueous ethanol extract powder of prune fruit Camu Camu fruit juice powder 2% Dragon fruit juice powder 2% Coconut milk 5% Aspartame 0.1% Thiamine hydrochloride 0.33% Riboflavin 0.33% Vitamin B6 0.17% Cyanocobalamin 0.17% Fragrance 0.17% Reduced palatinose 6.67% Calcium stearate 3.33% Hydrodoxypropylcellulose Remainder

[0085] [Example 28] (Manufacturing of chewable tablets) Chewable tablets (1000 mg per tablet) consisting of the following ingredients were manufactured. The resulting chewable tablets have an improved taste characteristic of Terminalia (bitterness, astringency, etc.), and excellent anti-obesity effects can be obtained by taking 2 tablets three times a day (6 tablets in total).

[0086] Terminalia bellirica fruit hydrated ethanol extract powder 10% 15% Hot Water Extract Powder of Prune Fruit 1% crushed kale leaves Sucralose 0.1% Aspartame 0.05% Vitamin B1 0.01% Vitamin B2 0.01% Vitamin B6 0.01% Fragrance 0.01% Calcium stearate 1.00% Silicon dioxide 2.00% Hydroxypropylcellulose 1.00% Cellulose 30.00% Reduced palatinose (remaining portion)

[0087] [Example 29] (Manufacturing of packaged beverages) A 300mL containerized beverage consisting of the following ingredients was manufactured. The resulting containerized beverage showed improved characteristics of Terminalia (bitterness, astringency, etc.), and its consumption yielded excellent anti-obesity effects.

[0088] Water extract powder of Terminalia bellirica fruit 0.1% Dried dead red yeast rice powder 0.1% Pu-erh tea processed foods 0.13% Jasmine tea processed foods 0.15% Fructooligosaccharides 0.075% Vitamin C 0.25% water remainder [Industrial applicability]

[0089] The oral composition of the present invention has anti-obesity effects, and therefore has high industrial utility.

Claims

[Claim 1] An oral composition containing terminalia and mangosteen.

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