Preparation method of high-dispersity metal-polyphenol nano enzyme

Abstract

The invention relates to a preparation method of a high-dispersity metal-polyphenol nano enzyme, which comprises the following steps: dispersing a metal compound and a polyphenol compound in water, uniformly mixing and stirring to preliminarily form a dispersion liquid containing nano enzyme particles, and homogenizing; adding hydrolyzed corn starch into the homogenized dispersion liquid, and homogenizing again; and carrying out micro-jet refining treatment on the nano-enzyme dispersion liquid to finally obtain the dispersion liquid containing the high-dispersity metal-polyphenol nano-enzyme. Compared with the prior art, the prepared nano-enzyme dispersion liquid is uniform in particle size, transparent and clear, and the long-acting activity is remarkably enhanced.

Description

Technical Field

[0001] This invention relates to the field of nanozyme technology, and in particular to a method for preparing highly dispersed metal-polyphenol nanozymes. Background Technology

[0002] Nanozymes are a class of nanomaterials possessing natural enzyme activity or similar catalytic functions, and are widely used in fields such as biological detection, medical diagnostics, and antioxidation. Polyphenols, such as epigallocatechin gallate (EGCG), or tannins, react with metal ions (such as Cu). 2+ Zn 2+ They can combine to form complexes with peroxidase or superoxide dismutase-like activities.

[0003] However, during the synthesis of these metal-polyphenol nanozymes, precipitation and aggregation often occur due to metal-polyphenol complexation, making it difficult to form a clear, transparent, and homogeneous system, which severely affects their dispersibility and functional stability. Because of the precipitation and aggregation properties of these nanozymes, it is difficult to uniformly incorporate them into product formulations. Large-particle nanozymes cannot fully exert their effects, and the raw materials themselves may also experience caking, stratification, and surface oxidation and discoloration during storage due to precipitation and aggregation.

[0004] CN119456034A discloses an ultradispersed copper-based nanozyme, its preparation method, and its application. It employs traditional dispersants or surfactants such as vinylpyrrolidone, polyethylene glycol, and sodium dodecyl sulfate to enhance the dispersibility and stability of the copper-based nanozyme. However, while these commonly used traditional dispersants (including glycerol and propylene glycol) can improve the dispersibility of some systems, their effectiveness in improving the long-term stability and usability of metal-polyphenol nanozyme complexes remains very limited.

[0005] Therefore, there is an urgent need for a novel dispersion system to improve the long-term dispersion stability and usability of metal-polyphenol nanozymes. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the existing technology in terms of poor long-term stability and usability by providing a method for preparing highly dispersible metal-polyphenol nanozymes.

[0007] The objective of this invention can be achieved through the following technical solutions: This invention first provides a method for preparing highly dispersed metal-polyphenol nanozymes, the method comprising the following steps: S1: Disperse the metal compound and polyphenol compound in water, mix and stir evenly to initially form a dispersion containing nanoenzyme particles, and then perform homogenization treatment. S2: Add hydrolyzed corn starch to the homogenized dispersion and homogenize again; S3: The nanozyme dispersion is subjected to microfluidic refinement to finally obtain a dispersion containing highly dispersible metal-polyphenol nanozymes.

[0008] Further, in step S1, the mass ratio of the metal compound to the polyphenol compound is 1:(0.02-0.15).

[0009] Further, in step S1, the metal compound is any one or more of copper-containing compounds, zinc-containing compounds, iron-containing compounds, magnesium-containing compounds, calcium-containing compounds, and manganese-containing compounds.

[0010] Furthermore, the copper-containing compound is any one or more of tripeptide-1 copper, copper gluconate, copper sulfate, copper chloride, copper aspartate, copper chlorophyll, copper acetate, or copper chlorophyllin, preferably copper gluconate.

[0011] Furthermore, the zinc-containing compound is any one or more of zinc pyrrolidone carboxylate, zinc gluconate, zinc lactate, zinc aspartate, zinc glycine, zinc hydrolyzed hyaluronic acid, zinc hydrolyzed collagen, zinc DNA, zinc acetate, zinc sulfate, or zinc chloride, preferably zinc gluconate.

[0012] Furthermore, the iron-containing compound is any one or more of ferrous sulfate, ferric chloride, ferric citrate, and ferric lactate.

[0013] Furthermore, the magnesium-containing compound is any one or more of magnesium sulfate, magnesium chloride, magnesium nitrate, magnesium glutamate, magnesium malate, magnesium lactate, magnesium taurate, and magnesium gluconate.

[0014] Furthermore, the manganese-containing compound is any one or more of manganese sulfate, manganese stearate, manganese lactate, manganese chloride, manganese nitrate, and manganese dihydrogen phosphate.

[0015] Furthermore, the calcium-containing compound is any one or more of calcium chloride, calcium lactate, calcium gluconate, calcium nitrate, and calcium bicarbonate.

[0016] Further, in step S1, the polyphenolic compound is any one or more of tannic acid, resveratrol, tea polyphenols, ferulic acid, caffeic acid, chlorogenic acid, gallic acid, proanthocyanidins, epigallocatechin gallate, gallic acid, curcumin, and quercetin.

[0017] Furthermore, in step S1, the metal compound and polyphenol compound are derived from natural plants, fermented sources, or artificially synthesized.

[0018] Furthermore, in step S1, the pH of the dispersion is 4-7 to promote the uniformity of complexation.

[0019] Further, in step S1, the rotation speed of the homogenization process is 12000-18000 rpm, preferably 15000 rpm.

[0020] Furthermore, in step S1, the homogenization process takes 4-10 minutes, preferably 5 minutes.

[0021] Furthermore, in step S1, the dispersion also includes a stabilizer.

[0022] Furthermore, the stabilizer is any one or more of sodium metabisulfite, ascorbic acid, citric acid, sodium sulfite, and disodium EDTA. When used in conjunction with hydrolyzed corn starch, it can enhance the stability of the nanozyme and prevent deterioration and discoloration during storage.

[0023] Furthermore, the mass ratio of the stabilizer to the polyphenol compound is (0.2-1):1.

[0024] Further, in step S2, the final concentration of the hydrolyzed corn starch in the dispersion is 10-50 wt%, preferably 20-50 wt%.

[0025] Further, in step S2, the rotation speed of the homogenization process is 12000-18000 rpm, preferably 15000 rpm.

[0026] Further, in step S2, the homogenization process takes 4-10 minutes, preferably 5 minutes.

[0027] Furthermore, in step S3, the pressure of the microjet refinement process is set to 150-250 MPa.

[0028] Further, in step S3, the final concentration of the highly dispersed metal-polyphenol nanozyme is 30-120 mg / mL.

[0029] Furthermore, in step S3, the particle size range of the highly dispersed metal-polyphenol nanozyme is 30-200 nm.

[0030] The present invention also provides a highly dispersible metal-polyphenol nanozyme, which is obtained by any of the preparation methods described above.

[0031] Compared with the prior art, the present invention has the following technical advantages: (1) This invention addresses the problems of easy aggregation and precipitation, poor dispersibility, and easy oxidation in traditional nanozyme systems. It innovatively uses hydrolyzed corn starch as a dispersant and stabilizer. By adjusting the pH value, mechanical homogenization, and microfluidic treatment, the nanozyme dispersion is made to have uniform particle size, clear transparency, and significantly enhanced antioxidant properties. Compared with traditional dispersion systems such as PEG400, propylene glycol, SDS, and PVP, hydrolyzed corn starch exhibits superior performance in terms of stability, dispersibility, antibacterial properties, and oxidation inhibition of metal-polyphenol nanozymes.

[0032] (2) The highly dispersible nanozyme obtained by adding hydrolyzed corn starch as a dispersant in this invention is not only clear and transparent in appearance and uniformly dispersed, but also brings a significant improvement in antioxidant capacity and antibacterial performance. It can maintain long-term antioxidant and long-term antibacterial effects for 30 days, bringing unexpected and significant effects in terms of preparation process and performance.

[0033] (3) The hydrolyzed corn starch dispersant of the present invention is naturally sourced, environmentally friendly, and biocompatible, making it suitable for applications in pharmaceuticals, cosmetics, food and other fields.

[0034] (4) The preparation process of the highly dispersed metal-polyphenol nanozyme of the present invention is simple and can be mass-produced. Attached Figure Description

[0035] Figure 1 The images show the stability of the nanoenzyme dispersions of Examples 1-5 of the present invention (from left to right, Examples 1-5).

[0036] Figure 2 The images show the stability of the nanoenzyme dispersions of Comparative Examples 1-6 and Example 3 of the present invention (from left to right: Comparative Examples 1-6 and Example 3).

[0037] Figure 3 The results are from the DPPH clearance rate test.

[0038] Figure 4 The results are the DPPH clearance rate test results after 30 days. Detailed Implementation

[0039] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. These embodiments are based on the technical solution of the present invention and provide detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following embodiments.

[0040] Unless otherwise specified, the reagents, methods, instruments, and equipment used in this invention are conventional in the art. Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0041] Example 1: This embodiment provides a highly dispersible nanozyme based on the complexation of epigallocatechin gallate (EGCG) and copper gluconate. The specific preparation method is as follows: (1) Solution preparation: Weigh 5 g of EGCG and dissolve it in 100 mL of deionized water; weigh 50 g of copper gluconate and dissolve it in 400 mL of deionized water. Mix the two liquids together and adjust the pH to about 5.6 by adding sodium hydroxide dropwise while stirring for 5-10 minutes.

[0042] (2) Add stabilizer: Weigh 2.5 g sodium metabisulfite and dissolve it in 100 mL of deionized water. Add it to the above mixture and the pH drops to about 5.4. Continue to stir until homogeneous.

[0043] (3) Volume adjustment and mechanical homogenization: Add deionized water to make up to a total mass of 900 g, and use a mechanical homogenizer (speed controlled at 15000 rpm) for 5 minutes for homogenization.

[0044] (4) Add dispersant: Add 100 g of hydrolyzed corn starch (corresponding to a final concentration of 10%) to the above liquid, process it again with a mechanical homogenizer for 5 minutes, and then let it stand for 30 minutes.

[0045] (5) Microfluidic refinement: The particle size was refined using a microfluidic device with a pressure of 180 MPa. The process was repeated twice to obtain a clear, transparent nanoenzyme dispersion with uniform particle size.

[0046] Example 2: This embodiment provides a highly dispersible nanozyme based on EGCG and copper gluconate complexation. The difference from Example 1 is that in step (3), deionized water is added to make up to a total mass of 800 g, and the amount of hydrolyzed corn starch added is 200 g, corresponding to a final concentration of 20% for the hydrolyzed corn starch.

[0047] The dispersion in this embodiment has a particle size range of 30-200 nm, the solution is clear without precipitation, and there is no obvious oxidation or discoloration after 48 hours.

[0048] Example 3: This embodiment provides a highly dispersible nanozyme based on EGCG and copper gluconate complexation. The difference from Example 1 is that in step (3), deionized water is added to make up to a total mass of 650 g, and the amount of hydrolyzed corn starch added is 350 g, corresponding to a final concentration of 35% for the hydrolyzed corn starch.

[0049] The dispersion in this embodiment has a particle size range of 30-200 nm, the solution is clear without precipitation, and there is no obvious oxidation or discoloration after 48 hours.

[0050] Example 4: This embodiment provides a highly dispersible nanozyme based on the complexation of EGCG and copper gluconate. The difference from Example 1 is that in step (1), the amount of EGCG added is 10 g, and the amount of copper gluconate added is 100 g. In step (3), deionized water is added to bring the total mass to 650 g, and the amount of hydrolyzed corn starch added is 350 g, corresponding to a final concentration of 35% for the hydrolyzed corn starch.

[0051] The dispersion in this embodiment has a particle size range of 30-200 nm, the solution is clear without precipitation, and there is no obvious oxidation or discoloration after 48 hours.

[0052] Example 5: This embodiment provides a highly dispersible nanozyme based on the complexation of EGCG and copper gluconate. The difference from Example 1 is that in step (1), 10g of EGCG is weighed and dissolved in 50 mL of deionized water; 100g of copper gluconate is weighed and dissolved in 200 mL of deionized water. Deionized water is added to bring the total mass to 500g, and the amount of hydrolyzed corn starch added is 500g, corresponding to a final concentration of 50% for the hydrolyzed corn starch.

[0053] The dispersion in this embodiment has a particle size range of 30-200 nm, the solution is clear without precipitation, and there is no obvious oxidation or discoloration after 48 hours.

[0054] Example 6: This embodiment provides a highly dispersible nanozyme based on a complex of tannic acid and copper gluconate. The difference from Example 1 is that the polyphenolic compound used is tannic acid.

[0055] Example 7: This embodiment provides a highly dispersible nanozyme based on the complexation of EGCG and copper sulfate pentahydrate. The difference from Example 1 is that the copper-containing compound is copper sulfate pentahydrate.

[0056] Example 8: This embodiment provides a highly dispersible nanozyme based on the complexation of EGCG and zinc gluconate. The difference from Example 1 is that the metal salt is a zinc-containing compound, specifically zinc gluconate.

[0057] Example 9: This embodiment provides a highly dispersible nanozyme based on the complexation of EGCG and zinc pyrrolidone carboxylate. The difference from Example 1 is that the metal salt is a zinc-containing compound, specifically zinc pyrrolidone carboxylate.

[0058] Example 10: This embodiment provides a highly dispersible nanozyme based on EGCG complexed with an iron-containing compound. The difference from Example 1 is that the metal salt is an iron-containing compound, specifically ferric chloride.

[0059] Example 11: This embodiment provides a highly dispersible nanozyme based on EGCG complexed with a magnesium-containing compound. The difference from Example 1 is that the metal salt is a magnesium-containing compound, specifically magnesium chloride.

[0060] Example 12: This embodiment provides a highly dispersible nanozyme based on EGCG complexed with a manganese-containing compound. The difference from Example 1 is that the metal salt is a manganese-containing compound, specifically manganese sulfate.

[0061] Example 13: This embodiment provides a highly dispersible nanozyme based on EGCG complexed with a calcium-containing compound. The difference from Example 1 is that the metal salt is a calcium-containing compound, specifically calcium chloride.

[0062] Table 1 summarizes the state and dispersion time of different amounts of hydrolyzed corn starch and different concentrations of nanozyme dispersions in Examples 1-5.

[0063] Table 1. Dispersion states and dispersion times for Examples 1-5 From Table 1 and Figure 1 It is known that when the amount of hydrolyzed corn starch added is within the preferred concentration range of 20-50 wt%, a uniformly dispersed and transparent nanoenzyme dispersion can be obtained, and no obvious oxidation or discoloration will occur over a long period of time. However, when the amount of hydrolyzed corn starch added is small (10 wt%), the nanoenzyme dispersion appears turbid.

[0064] Examples 6-13 also yielded highly dispersible nanozymes that were transparent, clear, and uniformly dispersed under the reaction of different metal compounds with polyphenols.

[0065] Comparative Example 1: This comparative example provides a nanozyme based on the complexation of EGCG and copper gluconate. The difference from Example 3 is that in this comparative example, deionized water is directly added to make up to a total mass of 1000 g, and hydrolyzed corn starch is not added.

[0066] Comparative Example 2: This comparative example provides a nanozyme based on EGCG and copper gluconate complexation. The difference from Example 3 is that hydrolyzed corn starch is replaced with PEG400 in this comparative example.

[0067] Comparative Example 3: This comparative example provides a nanozyme based on EGCG and copper gluconate complexation. The difference from Example 3 is that hydrolyzed corn starch is replaced with propylene glycol in this comparative example.

[0068] Comparative Example 4: This comparative example provides a nanozyme based on EGCG and copper gluconate complexation. The difference from Example 3 is that deionized water was added to make up the total mass to 800 g, and 200 g of glycerol was added.

[0069] Comparative Example 5: This comparative example provides a nanozyme based on EGCG and copper gluconate complexation. The difference from Example 3 is that deionized water was added to make up the total mass to 990 g, and 10 g of sodium dodecyl sulfate was added.

[0070] Comparative Example 6: This comparative example provides a nanozyme based on EGCG and copper gluconate complexation. The difference from Example 3 is that deionized water was added to make up the total mass to 980 g in this comparative example, and 20 g of polyvinylpyrrolidone was added.

[0071] The dispersing effects and stability of the dispersants used in Comparative Examples 1-6 and the hydrolyzed corn starch used in this invention are summarized in Table 2.

[0072] Table 2. Dispersion effect and long-term stability of comparative examples 1-6 Combine Table 2 and Figure 2 It can be seen that when no dispersant is added ( Figure 2 (Left 1) The entire nanozyme system showed significant precipitation and poor dispersion uniformity. When PEG400 (…) was added… Figure 2 Polyether dispersants, represented by the second from the left, and propylene glycol ( Figure 2 Left 3), Glycerin ( Figure 2 After adding polyol dispersants, such as the one represented by the fourth one from the left, the dispersibility of nanozymes showed almost no improvement, still exhibiting obvious precipitation and reddish tinge. When SDS (4) was added... Figure 2 When an ionic surfactant (e.g., left 5) was used, the entire nanozyme system exhibited severe foaming and remained turbid. When PVP (…) was added… Figure 2 When using a polymeric dispersant (e.g., left 6), the precipitation condition improved, but the entire nanozyme system remained turbid and showed poor stability. Only when hydrolyzed corn starch (…) was added… Figure 2 Only when the left 7) is a clear, transparent, highly dispersible nanozyme with good dispersibility and stability can be obtained.

[0073] Based on the successful preparation of highly dispersed nanozymes, this invention further explored the long-term functional activity of highly dispersed nanozymes, specifically including the following tests: (1) DPPH antioxidant experiment: 3.94 mg of DPPH was dissolved in 50 mL of methanol to obtain a 0.2 mmol / L DPPH solution, which was stored at 4 °C protected from light for later use. The test consisted of a blank control group, a sample blank group, and a sample group. The blank control group consisted of methanol + DPPH, the sample blank group consisted of sample + methanol, and the sample group consisted of sample + DPPH. 180 µL of DPPH working solution was added to each well of the plate, followed by 20 µL of sample solution. After mixing, the plate was incubated at room temperature protected from light for 30 min. The absorbance at 562 nm was then measured in each well, and the DPPH scavenging rate was calculated using the following formula: DPPH scavenging rate = (absorbance of sample group - absorbance of sample blank group) / absorbance of blank control group.

[0074] After the test is completed, each sample is placed at room temperature and protected from light for 30 days, and its antioxidant activity is tested again according to the above steps.

[0075] (2) Sterilization test: This invention employs the minimum bactericidal concentration (MBC) test to determine the antibacterial activity of metal-polyphenol nanozymes before and after the addition of a dispersant.

[0076] The strain used for the MBC test was *Malassezia ATCC44344*, and the culture medium was MH broth. First, the sample solutions (the original concentrations of the nanozymes in Comparative Examples 1-6 and Example 3 were all diluted to 50 mg / mL) were serially diluted twofold in test tubes with MH broth to obtain a series of solutions of different concentrations for later use. The bacterial culture was then prepared and diluted to a final inoculum concentration of approximately 5 × 10⁻⁶. 5 CFU / mL, add the bacterial suspension to each concentration of drug solution, and incubate in a constant temperature incubator at 35±1℃ for 16-20 hours. Visually inspect the tube containing the lowest drug concentration; the tube with no bacterial growth is the minimum inhibitory concentration (MIC) of the test bacteria.

[0077] Take 0.1 mL of culture from each tube showing no visible growth (usually tubes with concentrations at or above the MIC value) and spread it onto fresh, drug-free LB agar plates. Incubate at 37 °C for 24 hours. After incubation, count the colonies on the agar plates and determine the minimum drug concentration that kills more than 99.9% (i.e., reduces the original inoculum by 3 orders of magnitude) as the MBC value. A lower MBC value indicates better bactericidal activity.

[0078] After the test is completed, each sample is placed at room temperature and protected from light for 30 days, and the antibacterial activity against Malassezia is tested again following the above steps.

[0079] Depend on Figure 3 The DPPH scavenging test results show that the DPPH scavenging rate of nanozymes dispersed with hydrolyzed corn starch can be increased from approximately 43% to as high as 91%, and the antioxidant capacity is significantly higher than that of other dispersant formulations. The main reason is that the nanozymes are thoroughly dispersed, which increases the overall surface area of ​​the nanozymes, allowing them to fully contact the reactants and thus exert their antioxidant activity.

[0080] Depend on Figure 4 The DPPH scavenging test results after 30 days showed that the DPPH scavenging rate of the nanoenzyme dispersed with hydrolyzed corn starch decreased only from 91% to 85%, and the antioxidant activity decreased only slightly, but was still significantly higher than other dispersant formulations. Meanwhile, the antioxidant activity of the nanoenzyme stock solution further decreased from 44% to 24%. These results indicate that the nanoenzyme of this invention, after adding hydrolyzed corn starch, still possesses long-lasting antioxidant activity after 30 days of storage.

[0081] Table 3 Summary of MBC values ​​(unit: mg / mL) As summarized in Table 3, the original MBC value of the EGCG-Cu nanozyme was 12.5 mg / mL. Only the nanozymes with added SDS and hydrolyzed corn starch showed improved bactericidal performance. The MBC value decreased from 12.5 mg / mL to 6.25 mg / mL after adding SDS, and further decreased to 1.56 mg / mL after dispersing with hydrolyzed corn starch. Furthermore, separate tests of the bactericidal activity of SDS and hydrolyzed corn starch showed that SDS itself had an MBC as low as 0.05 mg / mL, exhibiting antibacterial effects. This indicates that the enhanced antibacterial effect after adding SDS mainly comes from SDS itself. Hydrolyzed corn starch itself has no antibacterial effect; the significant enhancement of the antibacterial effect in the hydrolyzed corn starch group mainly comes from the thorough dispersion of the nanozyme.

[0082] Furthermore, when the samples were stored for 30 days, the antibacterial performance was tested. The results showed that the antibacterial activity of the nanozyme stock solution and the nanozyme formulation with other dispersants decreased. However, the antibacterial activity after adding hydrolyzed corn starch did not change significantly, indicating that the nanozyme of the present invention with added hydrolyzed corn starch also has long-lasting antibacterial activity.

[0083] Therefore, the highly dispersible nanozyme obtained by adding hydrolyzed corn starch as a dispersant in this invention is not only clear and transparent in appearance and uniformly dispersed, but also significantly improves antioxidant capacity and antibacterial properties. It can maintain long-term antioxidant and antibacterial effects for 30 days. It brings unexpected and significant effects in terms of preparation process and performance, and is suitable for applications in pharmaceutical, cosmetic, and food fields.

[0084] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. A method for preparing a highly dispersible metal-polyphenol nanozyme, characterized in that, The preparation method includes the following steps: S1: Disperse the metal compound and polyphenol compound in water, mix and stir evenly to initially form a dispersion containing nanoenzyme particles, and then perform homogenization treatment. S2: Add hydrolyzed corn starch to the homogenized dispersion and homogenize again; S3: The nanozyme dispersion is subjected to microfluidic refinement to finally obtain a dispersion containing highly dispersible metal-polyphenol nanozymes.

2. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S1, the mass ratio of the metal compound to the polyphenol compound is 1:(0.02-0.15).

3. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S1, the metal compound is any one or more of the following: copper-containing compounds, zinc-containing compounds, iron-containing compounds, magnesium-containing compounds, calcium-containing compounds, and manganese-containing compounds. The polyphenolic compound is any one or more of the following: tannic acid, resveratrol, tea polyphenols, ferulic acid, caffeic acid, chlorogenic acid, gallic acid, proanthocyanidins, epigallocatechin gallate, gallic acid, curcumin, and quercetin. The metal compounds and polyphenolic compounds are derived from natural plants, fermented sources, or artificially synthesized.

4. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S1, the pH of the dispersion is 4-7; The homogenization process is carried out at a rotation speed of 12,000-18,000 rpm; The homogenization process takes 4-10 minutes.

5. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S1, the dispersion also includes a stabilizer; The stabilizer is any one or more of sodium metabisulfite, ascorbic acid, citric acid, sodium sulfite, and disodium ethylenediaminetetraacetate. The mass ratio of the stabilizer to the polyphenol compound is (0.2-1):

1.

6. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S2, the final concentration of the hydrolyzed corn starch in the dispersion is 10-50 wt%.

7. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S2, the rotation speed of the homogenization process is 12000-18000 rpm; The homogenization process takes 4-10 minutes.

8. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S3, the pressure of the microjet refinement process is set to 150-250 MPa.

9. The method for preparing highly dispersed metal-polyphenol nanozymes according to claim 1, characterized in that, In step S3, the final concentration of the highly dispersed metal-polyphenol nanozyme is 30-120 mg / mL; The particle size range of the highly dispersed metal-polyphenol nanozyme is 30-200 nm.

10. A highly dispersible metal-polyphenol nanozyme, characterized in that, It is obtained by the preparation method according to any one of claims 1-9.

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