Additives for mushroom culture media and methods for producing mushroom culture media

By combining a bulking agent and antacid in mushroom cultivation media, the issue of non-uniform antacid dispersion and measurement errors is addressed, resulting in improved mushroom growth and yield.

JP7850115B2Active Publication Date: 2026-04-22SETOLAS HLDG INC
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
SETOLAS HLDG INC
Filing Date
2023-09-15
Publication Date
2026-04-22

AI Technical Summary

Technical Problem

Existing mushroom cultivation media face issues with non-uniform dispersion of antacids leading to localized high concentrations, which can adversely affect mushroom growth and yield, and measurement errors in the addition of antacids.

Method used

Incorporating a bulking agent and an antacid into the culture medium, where the bulking agent includes materials like sawdust and the antacid includes aluminum, calcium, and magnesium compounds, to improve uniform mixing and reduce measurement errors.

Benefits of technology

The additive enhances uniform mixing and reduces measurement errors in the culture medium, promoting consistent mushroom growth and yield by ensuring even distribution of antacids.

✦ Generated by Eureka AI based on patent content.

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Abstract

To provide an additive for a mushroom culture medium, the additive being capable of reducing a weighing error and improving the uniform mixing property of an antacid in a culture medium.SOLUTION: An additive for a mushroom culture medium of the present disclosure includes an extender and an antacid.SELECTED DRAWING: None
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Description

Technical Field

[0001] The present disclosure relates to an additive for a mushroom culture medium and a method for producing a mushroom culture medium.

Background Art

[0002] Patent Document 1 describes a mushroom cultivation medium in which shell fossil powder is formed into granules having a diameter of about 1 to 5 mm, and 0.3 to 3% of the weight of the medium is added with the shell fossil powder granules.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] In the mushroom cultivation medium described in Patent Document 1, there may be cases where the fruit body yield corresponding to the added amount of shell fossil powder cannot be obtained.

[0005] According to the studies of the present inventors, when the addition amount of the antacid in the mushroom culture medium becomes excessive, it may have an adverse effect on the mushroom growth promoting effect. In addition, in the mushroom culture medium, when the antacid is not sufficiently uniformly dispersed, the concentration of the antacid becomes locally high, and it may be difficult to obtain the mushroom growth promoting effect expected from the addition amount of the antacid. The present disclosure aims to provide an additive for a mushroom culture medium that can reduce the measurement error and improve the uniform mixing property of the antacid in the culture medium.

Means for Solving the Problems

[0006] The first embodiment of the present disclosure provides an additive for a mushroom culture medium, which includes an extender and an antacid.

[0007] In a second embodiment of the present disclosure, in the first embodiment, the bulking agent may include one or more selected from the group consisting of sawdust, corn cob meal, bagasse, beet meal, okara, coffee grounds, soybean hulls, rice bran, wheat bran, and cotton hull.

[0008] In a third embodiment of this disclosure, the antacid may include an aluminum compound in any one of the first and second embodiments.

[0009] In a fourth embodiment of this disclosure, in any one of the first to third embodiments, the antacid may further include one or more selected from calcium compounds, magnesium compounds, and sodium compounds.

[0010] In the fifth embodiment of this disclosure, in any one of the first to fourth embodiments, the content of aluminum atoms in the antacid may be 60% by weight or less in terms of oxides, based on the total weight of the antacid.

[0011] In the sixth embodiment of this disclosure, in any one of the first to fifth embodiments, the calcium atom content in the antacid may be 60% by weight or less in terms of oxides, based on the total weight of the antacid.

[0012] In the seventh embodiment of this disclosure, in any one of the first to sixth embodiments, the magnesium atom content in the antacid may be 30% by weight or less in terms of oxides, based on the total weight of the antacid.

[0013] In the eighth embodiment of this disclosure, in any one of the first to seventh embodiments, the dry weight ratio of the bulking agent to the antacid agent may be 99.5:0.5 to 10:90.

[0014] In the ninth embodiment of this disclosure, in any one of the first to eighth embodiments, the angle of repose of the mushroom culture medium additive may be 50 degrees or less.

[0015] The tenth embodiment of the present disclosure includes a step of mixing an extender and an antacid to obtain an additive for a mushroom culture medium, and a step of mixing the additive for the mushroom culture medium and a culture medium raw material to obtain a mushroom culture medium, and provides a method for producing a mushroom culture medium.

Advantages of the Invention

[0016] According to the present disclosure, it is possible to provide an additive for a mushroom culture medium that can reduce the measurement error of the antacid and improve the uniform mixing property in the culture medium.

Brief Description of the Drawings

[0017] [Figure 1] FIG. 1 is a schematic diagram for explaining a method for producing a mushroom culture medium.

Embodiments for Carrying Out the Invention

[0018] (First Embodiment: Additive for Mushroom Culture Medium) The additive for a mushroom culture medium of the present embodiment includes an extender and an antacid. Since the additive for a mushroom culture medium of the present embodiment includes an extender and an antacid, it is possible to reduce the measurement error and improve the uniform mixing property in the culture medium. Although the present disclosure should not be construed as being limited to a specific theory, the reason why the additive for a mushroom culture medium of the present disclosure can achieve such an effect is considered as follows.

[0019] According to the study by the present inventors, it is considered that when mixing an antacid and a culture medium raw material, the difference in fluidity between the antacid and the culture medium raw material may affect the uniform mixing property. However, it has been found that when an antacid and an extender are mixed in advance, the extender and the antacid can be uniformly dispersed, and moreover, an additive for a mushroom culture medium having high fluidity can be obtained. In addition, since the additive for a mushroom culture medium includes an extender and an antacid, the measurement error can also be reduced. As a result, it is considered that an additive for a mushroom culture medium that can reduce the measurement error and improve the uniform mixing property in the culture medium has been realized.

[0020] The above-mentioned bulking agent may be any material that does not adversely affect the growth of the mushroom fruiting body when used as an antacid. If it has an adverse effect on the growth of the mushroom fruiting body, for example, it may include the case where the yield of the mushroom fruiting body decreases.

[0021] In one aspect, it is preferable that the above-mentioned bulking agent does not elute aluminum, magnesium, and calcium even when in contact with an acid that the mushroom can produce. Thereby, the effect of increasing the yield of the mushroom fruiting body by the antacid can be appropriately exerted.

[0022] In such an aspect, specifically, when a mixed solution obtained by adding 85 parts by weight of ion-exchanged water to 15 parts by weight of the bulking agent is shaken at 160 rpm / min for 1 hour, the total elution amount of aluminum, magnesium, and calcium is preferably 3000 mg / 100 g or less per 100 g of the bulking agent. The total elution amount may be more preferably 0 mg / 100 g or more and 1000 mg / 100 g or less, and even more preferably 0 mg / 100 g or more and 550 mg / 100 g or less. In the above case, the elution amount of calcium is 0 mg / 100 g or more and 3000 mg / 100 g or less, and the preferable upper limit is 1000 mg / 100 g or less, more preferably 550 mg / 100 g or less. In the above case, the elution amount of magnesium is 0 mg / 100 g or more and 3000 mg / 100 g or less, and the preferable upper limit is 1000 mg / 100 g or less, more preferably 550 mg / 100 g or less. In the above case, the elution amount of aluminum is 0 mg / 100 g or more and 1000 mg / 100 g or less, and the preferable upper limit is 500 mg / 100 g or less, more preferably 100 mg / 100 g or less. By setting it within such a range, the effect of the antacid can be surely exerted.

[0023] In another embodiment, the bulking agent may preferably include one or more selected from the group consisting of sawdust, corn cob meal, bagasse, beet meal, okara, coffee grounds, soybean hulls, rice bran, wheat bran, and cotton hull, and more preferably may include sawdust.

[0024] In this embodiment, the total content of sawdust, corn cob meal, bagasse, beet meal, okara, coffee grounds, soybean hulls, rice bran, wheat bran, and cotton hull in 100 parts by weight of the total amount of the above-mentioned bulking material may preferably be 80 parts by weight or more and 100 parts by weight or less, more preferably 90 parts by weight or more and 100 parts by weight or less, and even more preferably 95 parts by weight or more and 100 parts by weight or less.

[0025] In this disclosure, content may be based on dry weight. In this disclosure, dry weight means the weight measured immediately after drying the object at 105°C for 3 hours, taking care to avoid moisture absorption.

[0026] The content of the above-mentioned bulking agent may be preferably 10% to 99.5% by weight, more preferably 13% to 99.2% by weight, even more preferably 17% to 99% by weight, even more preferably 33% to 98.4% by weight, and even more preferably 50% to 98% by weight, based on 100% by weight of the total amount of the above-mentioned mushroom culture substrate additives.

[0027] The above-mentioned antacids refer to compounds that can neutralize acids. During the process of cultivating mushrooms, organic acids such as oxalic acid may be produced from the mushroom mycelium. Antacids can promote the growth of mushroom fruiting bodies by neutralizing these organic acids. In one embodiment, the antacid may be a compound in which the pH of a 10% by weight aqueous dispersion of the antacid is higher than the pH of a 10% by weight aqueous dispersion of the nutrient described later. The pH of a 10% by weight aqueous dispersion of the antacid may be, for example, 5.5 to 13.0, and moreover, 6.0 to 12.0.

[0028] In one embodiment, the antacid may preferably contain an aluminum compound. In a more preferred embodiment, the antacid may contain an aluminum compound and one or more selected from calcium compounds, magnesium compounds, and sodium compounds. In an even more preferred embodiment, the antacid may contain an aluminum compound and one or more selected from calcium compounds and magnesium compounds.

[0029] The aluminum, calcium, magnesium, and sodium compounds mentioned above may be natural or synthetic products. These compounds may also be hydrates. The forms of these compounds may include, for example, gels, glass, porous materials, powders, and particles. In specific examples, antacids may contain two or more, three or more, or four or more of these compounds. In more specific examples, antacids may contain one to five of these compounds, preferably two to five or more, for example, three.

[0030] In one embodiment, preferably, the aluminum compound may comprise one or more selected from aluminum hydroxide, aluminum silicate, magnesium aluminometasilicate, calcium aluminosilicate, calcium aluminosilicate glass, calcium aluminate hydrate, dawsonite, zeolite, hydrocalmite, and hydrotalcite. More preferably, the aluminum compound may comprise one or more selected from aluminum hydroxide and aluminum silicate. Even more preferably, the aluminum compound may comprise aluminum hydroxide.

[0031] In one embodiment, preferably, the magnesium compound may comprise one or more selected from magnesium carbonate, magnesium hydroxide, magnesium oxide, magnesium silicate, magnesium aluminometasilicate, and hydrotalcite. More preferably, the magnesium compound may comprise one or more selected from magnesium silicate and magnesium hydroxide. Even more preferably, the magnesium compound may comprise magnesium hydroxide.

[0032] In one embodiment, preferably, the sodium compound may include one or more selected from sodium bicarbonate, sodium alginate, and dawsonite.

[0033] In one embodiment, preferably, the calcium compound may comprise one or more selected from calcium carbonate, calcium hydroxide, calcium silicate, calcium aluminosilicate, calcium silicate glass, calcium aluminate hydrate, and hydrocalmite. More preferably, the calcium compound may comprise calcium hydroxide.

[0034] In one embodiment, the total content of the aluminum compound, calcium compound, magnesium compound, and sodium compound may be preferably 80% to 100% by weight, more preferably 90% to 100% by weight, and even more preferably 95% to 100% by weight, based on 100% by weight of the total amount of the antacid.

[0035] In one embodiment, the antacid may preferably comprise an aluminum compound, a calcium compound, and a magnesium compound. More preferably, the antacid may comprise one or more of aluminum, calcium, and magnesium selected from hydroxides, silicates, metasilicates, carbonates, double hydroxides, carbonate hydroxides, oxides, bicarbonates, double hydroxides, and alginates, and even more preferably, aluminum hydroxide, calcium hydroxide, and magnesium hydroxide.

[0036] In one embodiment, the aluminum atom content in the antacid is preferably 60% by weight or less, more preferably 5% to 58% by weight, even more preferably 10% to 50% by weight, and even more preferably 15% to 40% by weight, based on the total weight of the antacid and expressed as oxide (Al2O3). The above percentage may preferably be 5% by weight or more, more preferably 10% by weight or more, and even more preferably 15% by weight or more. Alternatively, the above percentage may preferably be 50% by weight or less, more preferably 45% by weight or less, and even more preferably 40% by weight or less. When the above mushroom culture medium additive is used with Buna Shimeji mushrooms, the aluminum atom content in the above antacid is preferably 1% to 60% by weight, and more preferably 1% to 30% by weight, based on the total weight of the above antacid, in terms of oxide (Al2O3). When the above-mentioned mushroom culture medium additive is used for shiitake mushrooms, the aluminum atom content in the above-mentioned antacid may be preferably 1% to 60% by weight, and more preferably 1% to 50% by weight, based on the total weight of the above-mentioned antacid, in terms of oxide (Al2O3). When the above-mentioned mushroom culture medium additive is used for mushrooms, the aluminum atom content in the above-mentioned antacid may be preferably 1% to 60% by weight, and more preferably 1% to 50% by weight, based on the total weight of the above-mentioned antacid, in terms of oxide (Al2O3). When the above-mentioned mushroom culture medium additive is used for maitake mushrooms, the aluminum atom content in the above-mentioned antacid is preferably 1% by weight or more and 60% by weight or less, and more preferably 1% by weight or more and 58% by weight or less, based on the total weight of the above-mentioned antacid, in terms of oxide (Al2O3).

[0037] In this disclosure, the content of a specific atom in terms of oxide, based on the total weight of the antacid, means the value obtained by dividing the weight-based content of the compound containing the specific atom by the molecular weight of the compound containing the specific atom, when the total weight of the antacid is set to 100% by weight, and then further multiplying the result by the molecular weight of the oxide of the specific atom. When the specific atom is an aluminum atom, an aluminum compound is considered a compound containing the specific atom, and Al2O3 is considered the oxide. Similarly, when the specific atom is a calcium atom, a calcium compound is considered a compound containing the specific atom, and CaO is considered the oxide. When the specific atom is a magnesium atom, a magnesium compound is considered a compound containing the specific atom, and MgO is considered the oxide.

[0038] In one embodiment, the calcium atom content in the antacid is preferably 60% by weight or less, more preferably 1% to 50% by weight, even more preferably 5% to 35% by weight, and even more preferably 10% to 30% by weight, based on the total weight of the antacid, in terms of oxide (CaO). The above percentage may be preferably 1% by weight or more, more preferably 5% by weight or more, even more preferably 10% by weight or more, and even more preferably 15% by weight or more. Also, the above percentage may be preferably 60% by weight or less, more preferably 50% by weight or less, even more preferably 35% by weight or less, and even more preferably 30% by weight or less. When the above mushroom culture substrate additive is used for Buna Shimeji mushrooms, the calcium atom content in the antacid is preferably 1% to 60% by weight, and even more preferably 1% to 55% by weight, based on the total weight of the antacid, in terms of oxide (CaO). When the above-mentioned mushroom culture medium additive is used for shiitake mushrooms, the calcium atom content in the above-mentioned antacid is preferably 1% to 30% by weight, and more preferably 1% to 20% by weight, based on the total weight of the above-mentioned antacid, in terms of oxide (CaO). When the above mushroom culture medium additive is used with mushrooms, the calcium atom content in the above antacid is preferably 0% to 30% by weight, and more preferably 0% to 20% by weight, based on the total weight of the above antacid, in terms of oxide (CaO). When the above mushroom culture medium additive is used with maitake mushrooms, the calcium atom content in the above antacid is preferably 0% to 50% by weight, and more preferably 0% to 48% by weight, based on the total weight of the above antacid, in terms of oxide (CaO).

[0039] In one embodiment, the magnesium atom content in the antacid is preferably 30% by weight or less, more preferably 1% to 20% by weight, and even more preferably 2% to 16% by weight, based on the total weight of the antacid, in terms of oxide (MgO). The above percentage may be preferably 1% by weight or more, more preferably 2% by weight or more, even more preferably 4% by weight or more, and even more preferably 5% by weight or more. Alternatively, the above percentage may be preferably 30% by weight or less, more preferably 20% by weight or less, even more preferably 16% by weight or less, and even more preferably 14% by weight or less. When the above mushroom culture medium additive is used with Buna Shimeji mushrooms, the magnesium atom content in the above antacid is preferably 0% to 30% by weight, and more preferably 0% to 20% by weight, based on the total weight of the above antacid, in terms of oxide (MgO). When the above-mentioned mushroom culture medium additive is used for shiitake mushrooms, the magnesium atom content in the above-mentioned antacid is preferably 0% to 30% by weight, and more preferably 0% to 20% by weight, based on the total weight of the above-mentioned antacid, in terms of oxide (MgO). When the above mushroom culture medium additive is used for mushrooms, the magnesium atom content in the above antacid is preferably 0% to 30% by weight, and more preferably 0% to 20% by weight, based on the total weight of the above antacid, in terms of oxide (MgO). When the above-mentioned mushroom culture medium additive is used for maitake mushrooms, the magnesium atom content in the above-mentioned antacid is preferably 0% to 30% by weight, and more preferably 0% to 20% by weight, based on the total weight of the above-mentioned antacid, in terms of oxide (MgO).

[0040] The dry weight ratio (bulker:antacid) of the above-mentioned bulking agent to the above-mentioned antacid is preferably 99.5:0.5 to 10:90, more preferably 99.2:0.8 to 13:87, even more preferably 99:1 to 17:83, even more preferably 98.4:1.6 to 33:67, and even more preferably 98:2 to 50:50. By having the weight ratio of the above-mentioned bulking agent to the antacid within this range, the weighing error of the antacid raw material can be reduced and the uniform mixing in the culture medium can be improved.

[0041] In this disclosure, dry weight ratio means the ratio of the dry weights of two or more materials.

[0042] Of the total amount of the above mushroom culture substrate additives (100% by weight), the combined weight of the above bulking agent and the above antacid may preferably be 80% by weight or more and 100% by weight or less, more preferably 90% by weight or more and 100% by weight or less, and even more preferably 95% by weight or more and 100% by weight or less.

[0043] The mushroom culture medium additives of this disclosure may contain other components in addition to the above-mentioned bulking agent and antacid. Examples of such components include nutrients.

[0044] In one embodiment, the nutrient solution means a compound that contains one or more atoms selected from potassium, phosphorus, nitrogen, and sulfur atoms and can exhibit an effect of promoting the growth of mushroom fruiting bodies. In one embodiment, the pH of a 10% by mass aqueous dispersion of the nutrient solution may be, for example, 2.0 or more and less than 5.5, and more specifically, 3.0 or more and 5.0 or less.

[0045] In one embodiment, the nutrient solution may contain one or more compounds selected from phosphate compounds, nitrate compounds, and sulfate compounds. These compounds may be natural or synthetic. These compounds may also be hydrates. The form of these compounds may be, for example, a gel, glass, a porous material, a powder, or particles. In specific examples, the nutrient solution may contain two or more, three or more, or four or more of these compounds. In more specific examples, the nutrient solution may contain one to five of these compounds. Alternatively, the nutrient solution may contain only one compound.

[0046] In one embodiment, the phosphate compound may comprise one or more selected from monocalcium hydrogen phosphate, dicalcium hydrogen phosphate, calcium phosphate, octacalcium phosphate, magnesium phosphate, monocalcium hydrogen phosphate, magnesium dihydrogen phosphate, aluminum phosphate, and hydroxyapatite. Preferably, the phosphate compound may comprise one or more selected from monocalcium hydrogen phosphate and dicalcium hydrogen phosphate, and more preferably, it may comprise dicalcium hydrogen phosphate.

[0047] In one embodiment, the nitrate compound may comprise one or more selected from calcium nitrate, magnesium nitrate, and aluminum nitrate.

[0048] In one embodiment, the above sulfate compound may comprise one or more selected from calcium sulfate, magnesium sulfate, and aluminum sulfate.

[0049] In one embodiment, the nutritional supplement may preferably contain a phosphate compound, preferably a monohydrogen phosphate compound such as calcium monohydrogen phosphate and magnesium monohydrogen phosphate; and may contain one or more dihydrogen phosphate compounds selected from calcium dihydrogen phosphate and magnesium dihydrogen phosphate, more preferably a dihydrogen phosphate compound, and even more preferably calcium dihydrogen phosphate.

[0050] In one embodiment, the content of the phosphoric acid compound may be preferably 80% to 100% by weight, more preferably 90% to 100% by weight, and even more preferably 95% to 100% by weight, per 100 parts by weight of the nutrient solution.

[0051] The mushroom culture substrate additive of this disclosure contains the above-mentioned weight material and may have good fluidity. Therefore, it has good uniform mixing properties with the culture substrate raw materials described later.

[0052] In one embodiment, the angle of repose of the mushroom culture medium additive of the present disclosure may be preferably 25 degrees or more and 50 degrees or less, more preferably 27 degrees or more and 48 degrees or less, and even more preferably 30 degrees or more and 46 degrees or less. Having the angle of repose of the mushroom culture medium additive within this range improves the fluidity of the mushroom culture medium additive, making it easier to uniformly mix the mushroom culture medium additive with the culture medium raw materials.

[0053] The angle of repose can typically be understood as the angle that the surface of a powder layer makes with the horizontal plane when powder accumulates in a gravitational field to form a free surface. Therefore, a smaller angle of repose for a mushroom culture substrate additive indicates higher fluidity. In this disclosure, the angle of repose can be measured by the angle of the accumulation formed when the mushroom culture substrate additive is allowed to fall naturally from a height of 50 mm into a lower dish with a diameter of 80 mm.

[0054] In one embodiment, the ratio of the angle of repose of the mushroom culture substrate additive of this disclosure to the angle of repose of the weight material (angle of repose of mushroom culture substrate additive / angle of repose of weight material) may be preferably 0.95 times or more and 1.1 times or less, more preferably 0.98 times or more and 1.08 times or less, and even more preferably 1 time or more and 1.05 times or less. Having the above angle of repose ratio within this range allows for good fluidity of the mushroom culture substrate additive, making it easier to uniformly mix the mushroom culture substrate additive and the culture substrate raw material.

[0055] In one embodiment, the amount of water contained in the mushroom culture substrate additive of this disclosure may be preferably 0% to 30% by weight, more preferably 0% to 25% by weight, and even more preferably 0% to 20% by weight, based on 100% by weight of the total amount of the mushroom culture substrate additive. Having the water content within the above range can suppress the formation of clumps, and when mixing the mushroom culture substrate additive with the mushroom culture substrate raw materials, uniformity can be further improved. In this disclosure, the moisture content can be calculated from the weight loss obtained by drying the mushroom culture substrate additive at 105°C for 3 hours, and specifically, it can be calculated using the following formula. Moisture content (by weight) = (Weight before drying - Weight after drying) / Weight before drying × 100

[0056] In one embodiment, the bulk volume of the mushroom culture medium additive of the present disclosure is 2 cm³. 3 / g or more 10cm 3 / g or less, more preferably 3cm 3 / g or more 9cm 3 Less than or equal to / g, more preferably 4cm 3 / g or more 8cm 3 It may be less than / g. Having the bulk volume within the above range reduces the measurement error of the raw material. In this disclosure, the bulk volume can be measured by the volume obtained when 10 g of the sample is gently placed into a 100 mL graduated cylinder.

[0057] The mushroom culture medium additives disclosed herein can be used for cultivating various mushroom species. In one embodiment, the mushroom culture medium additives disclosed herein can be used for cultivating shiitake, hollyhock, enoki mushroom, buna shimeji, nameko, maitake, king oyster mushroom, lion's mane mushroom, or oyster mushroom, but are not limited to these.

[0058] (Second embodiment: Method for producing mushroom culture medium) Methods for producing mushroom culture media are also included in the technical scope of this disclosure.

[0059] The method for producing mushroom culture media described herein is: A process of mixing a bulking agent and an antacid to obtain an additive for mushroom culture media, and The process includes the step of mixing the aforementioned mushroom culture medium additive with culture medium raw materials to obtain a mushroom culture medium.

[0060] In the following, the process of mixing the bulking agent and the antacid to obtain the mushroom culture medium additive will also be referred to as the first mixing process, and the process of mixing the mushroom culture medium additive and the culture medium raw material to obtain the mushroom culture medium will also be referred to as the second mixing process.

[0061] The above-mentioned bulking agent has the same meaning as the bulking agent in the first embodiment. Similarly, the above-mentioned antacid has the same meaning as the antacid in the first embodiment. All matters described in the first embodiment can be applied to this embodiment.

[0062] In the first mixing step, a bulking agent and an antacid are mixed to obtain an additive for mushroom culture media. In the manufacturing method of this disclosure, a highly fluid additive for mushroom culture media can be obtained by mixing a bulking agent and an antacid. By further mixing such a mushroom culture media additive with culture media raw materials to produce a mushroom culture media, it is possible to reduce the weighing error of the antacid raw materials and improve uniform mixing in the culture media.

[0063] The content of the above-mentioned bulking agent may be preferably 10% to 99.5% by weight, more preferably 13% to 99.2% by weight, even more preferably 17% to 99% by weight, even more preferably 33% to 98.4% by weight, and even more preferably 50% to 98% by weight, based on 100% by weight of the total amount of the above-mentioned bulking agent and the above-mentioned antacid. The content of the above-mentioned bulking agent may be preferably 10% or more by weight, more preferably 13% or more by weight, even more preferably 17% or more by weight, even more preferably 33% or more by weight, even more preferably 50% or more by weight, and may be preferably 99.99% or less by weight, more preferably 99.8% or less by weight, even more preferably 99.5% or less by weight, even more preferably 99% or less by weight, and even more preferably 95% or less by weight, based on 100% by weight of the total amount of the above-mentioned mushroom culture substrate additive.

[0064] The weight ratio of the above-mentioned bulking agent to the antacid (bulking agent:antacid) is preferably 99.5:0.5 to 10:90, more preferably 99.2:0.8 to 13:87, even more preferably 99:1 to 17:83, even more preferably 98.4:1.6 to 33:67, and even more preferably 98:2 to 50:50. By having the weight ratio of the above-mentioned bulking agent to the antacid within this range, the measurement error of the additive for the mushroom culture medium can be reduced and the uniform mixing in the culture medium can be improved.

[0065] The mixing in the first mixing step can be carried out by means used in the production of mushroom culture media. Typical mixing means include, but are not limited to, shaking and stirring. In one embodiment, stirring is preferred as the mixing means. By employing stirring as the mixing means, thorough mixing can be achieved, and the uniformity of the resulting mushroom culture media can be further improved.

[0066] The above stirring can typically be carried out using a stirring device equipped with stirring blades. Examples of such stirring blades include helical ribbon blades and screws.

[0067] The temperature in the first mixing step is preferably 0°C to 60°C, more preferably 0°C to 40°C, and even more preferably 0°C to 45°C. The mixing time in the first mixing step is preferably 1 minute to 2 hours, more preferably 1 minute to 1 hour, and even more preferably 1 minute to 30 minutes.

[0068] In the second mixing step, the above-mentioned mushroom culture medium additive and the culture medium raw material are mixed to obtain a mushroom culture medium. Because the above-mentioned mushroom culture medium additive has improved fluidity, it reduces weighing errors and improves the uniform mixing of the components contained in the culture medium, particularly the antacid components.

[0069] The above-mentioned culture medium materials can be appropriately selected by those skilled in the art depending on the mushroom species and cultivation conditions. In one embodiment, the mushroom culture medium materials may include base materials such as sawdust (e.g., cedar sawdust), corn cob meal, bagasse, and beet meal, as well as nutrients such as rice bran, wheat bran, cotton hull, okara, coffee grounds, and soybean hulls. The composition of the above-mentioned bulking agent and the composition of the culture medium raw material may be the same or different.

[0070] The above-mentioned mushroom culture substrate additive may be in an amount of 10 to 90 parts by weight, more preferably 20 to 80 parts by weight, and even more preferably 30 to 70 parts by weight, based on 100 parts by weight of the total amount of the above-mentioned culture substrate raw materials.

[0071] The mixing in the second mixing step can be carried out by the same means as those described as the mixing means in the first mixing step.

[0072] In the second mixing step, water may be present when mixing the mushroom culture medium additive and the culture medium raw materials. Such presence of water can be achieved, for example, by supplying water to the mixture of the mushroom culture medium additive and the culture medium raw materials. The method of supplying water is not particularly limited, and it can typically be done by spraying water.

[0073] The temperature in the second mixing step is preferably 0°C to 60°C, more preferably 0°C to 40°C, and even more preferably 0°C to 45°C. The mixing time in the second mixing step is preferably 1 minute to 2 hours, more preferably 1 minute to 1 hour, and even more preferably 1 minute to 30 minutes.

[0074] The embodiment will be described in more detail below based on the mushroom culture medium manufacturing system schematically shown in Figure 1.

[0075] In the mushroom culture medium manufacturing system shown in Figure 1, first, the bulking agent stored in the bulking agent tank 2 and the antacid stored in the antacid tank 3 are put into the first stirring tank 4a of the first stirring device 10a. The added bulking agent and antacid are stirred by the first stirring blade 6a fixed to the first stirring shaft 5a, and the mushroom culture medium additive is manufactured. The mushroom culture medium additive is recovered from the bottom of the first stirring tank 4a and transported to the second stirring device 10b.

[0076] Next, the mushroom culture medium additive produced in the first stirring device 10a and the culture medium raw materials stored in the culture medium raw material tank 7 are added to the second stirring tank 4b of the second stirring device 10b. The added mushroom culture medium additive and culture medium raw materials are stirred by the second stirring blade 6b fixed to the second stirring shaft 5b. When stirring the mushroom culture medium additive and culture medium raw materials, water may be sprayed onto the mixture from the watering device 8. Mushroom culture medium is produced by stirring the mushroom culture medium additive and culture medium raw materials. The obtained mushroom culture medium is recovered from the bottom of the second stirring tank 4b and stored in the mushroom culture medium recovery container 9.

[0077] In Figure 1, the first stirring device 10a and the second stirring device 10b are different from each other, but the invention is not limited to this, and the same stirring device may be used as both the first stirring device 10a and the second stirring device 10b. Such an embodiment can be implemented, for example, by connecting the culture substrate raw material tank 7 and the first stirring device 10a. Specifically, after producing the mushroom culture substrate additive in the first stirring device 10a, the culture substrate raw material stored in the culture substrate raw material tank 7 is further added to the first stirring device 10a and stirred to produce the mushroom culture substrate.

[0078] In this embodiment, the bulking agent, antacid, and culture medium raw materials are stored in the bulking agent tank 2, the antacid tank 3, and the culture medium raw material tank 7, respectively, but the disclosure is not limited thereto. One or more selected from the bulking agent and antacid may be directly added to the first stirring tank 4a without passing through the bulking agent tank 2 and / or the antacid tank 3. Alternatively, the culture medium raw materials may be directly added to the second stirring tank 4b without passing through the culture medium raw material tank 7.

[0079] The recovered mushroom culture medium may be stored as is, or it may be filled into individual containers.

[0080] The present disclosure provides an additive for mushroom culture media that can reduce weighing errors and improve uniform mixing in the culture medium. The mushroom culture media additive of the present disclosure can be used for cultivating various mushroom species. In one embodiment, the mushroom culture media additive of the present disclosure can be used for cultivating shiitake, hollyhock, enoki mushroom, buna shimeji, nameko, button mushroom, maitake, king oyster mushroom, lion's mane mushroom, or oyster mushroom. [Examples]

[0081] The present invention will be further described in detail by the following examples, but the present invention is not limited thereto.

[0082] (Example 1) An antacid (hereinafter also referred to as "antacid 1") was prepared by mixing 18 parts by weight of aluminum hydroxide, 65 parts by weight of calcium hydroxide, and 17 parts by weight of magnesium hydroxide. Next, 95 parts by weight of cedar sawdust and 5 parts by weight of the above antacid were placed in a polypropylene container and thoroughly stirred to obtain the mushroom culture substrate additive of the present disclosure.

[0083] Next, 50 parts by weight of the mushroom culture medium additive of the present disclosure and 50 parts by weight of cedar sawdust as a culture medium raw material were placed in a polypropylene container and shaken for 1 minute at a rate of 220 times per minute to obtain the mushroom culture medium of the present disclosure. In this example, cedar sawdust was used for convenience in measuring the concentrations of aluminum, calcium, and magnesium, and the culture medium raw material is not limited to cedar sawdust.

[0084] (Bulk specific volume) The sample was gently poured into a 100 mL graduated cylinder, and its bulk volume was measured. The samples used were antacid 1, cedar sawdust, and the mushroom culture medium additive obtained in Example 1.

[0085] [Table 1]

[0086] (Measurement of moisture content) A clean weighing bottle was prepared, and only the weight of the weighing bottle was measured. Next, approximately 1.0 g of the sample was weighed into the weighing bottle, and the total weight of the weighing bottle and the sample (before drying) was measured. With the lid of the weighing bottle open, it was placed in a drying oven and dried at 105°C for 3 hours. After drying, the lid of the weighing bottle was closed, and it was allowed to cool naturally in a desiccator until it reached room temperature. After the temperature had cooled sufficiently, the total weight of the weighing bottle and the sample (after drying) was measured. The drying loss (moisture content) was calculated based on the following formula. Antacid 1, cedar sawdust, and the mushroom culture medium additive obtained in Example 1 were used as the samples.

[0087]

number

[0088] [Table 2]

[0089] (Measurement of angle of repose) As an angle of repose measuring device, a suspended electromagnetic vibration angle of repose measuring instrument TYPE AOR-14 manufactured by Tsutsui Rikagakukikai Co., Ltd. was used to measure the angle of the deposit formed when the mushroom culture medium additive was allowed to fall naturally from a height of 50 mm onto an 80 mm diameter lower pan. Antacid 1, cedar sawdust, and the mushroom culture medium additive obtained in Example 1 were used as samples, and the ratio of the angle of repose of the mushroom culture medium additive to the angle of repose of the cedar sawdust (angle of repose of mushroom culture medium additive / angle of repose of cedar sawdust) was calculated.

[0090] [Table 3]

[0091] (Comparative Example 1) 2.5 parts by weight of antacid 1 and 97.5 parts by weight of cedar sawdust as a culture medium raw material were placed in a polypropylene container and shaken for 1 minute at a rate of 220 times per minute to obtain the culture medium of the comparative example.

[0092] (Measurement of concentration) In Example 1 and Comparative Example 1, 0.5 g each of the mushroom culture medium from Example 1 or the culture medium from Comparative Example 1 was sampled from the top, middle, and bottom of the polypropylene container after shaking. These samples were dissolved using the microwave method and made up to 50 mL. The resulting solution was diluted to a concentration measurable by inductively coupled plasma emission spectrometry (ICP), and the concentrations of aluminum, calcium, and magnesium were measured. For Example 1, the obtained concentrations were converted to the content in the mushroom culture medium additive, and for Comparative Example 1, they were converted to the content in antacid 1. The uniform dispersibility was then evaluated as an oxide equivalent value.

[0093] The results are shown in Table 1. In Table 1, Al2O3 represents the oxide equivalent of the aluminum content, CaO represents the oxide equivalent of the calcium content, and MgO represents the oxide equivalent of the magnesium content.

[0094] [Table 4]

[0095] When 2.5 parts by weight of antacid 1 and 97.5 parts by weight of cedar sawdust are uniformly mixed, the theoretical values ​​of the oxide content of each element are 0.243% by weight for aluminum (Al2O3 equivalent), 1.242% by weight for calcium (CaO equivalent), and 0.296% by weight for magnesium (MgO equivalent).

[0096] Example 1 is an example of the present disclosure, and it was confirmed that the content of each element was approximately the same as the theoretical value when the mushroom culture medium was sampled from the top, middle, or bottom. From this, it can be said that the mushroom culture medium additive of the present disclosure can reduce measurement errors and improve uniform mixing in the mushroom culture medium.

[0097] Comparative Example 1 is an example where the antacid and culture medium raw materials were directly mixed. It was confirmed that the content of aluminum, calcium, and magnesium was low at the top of the mixing container, and high at the bottom of the mixing container. From this, it can be said that when the antacid and culture medium raw materials are directly mixed, the uniformity of mixing in the mushroom culture medium is poor, and weighing errors may also occur. [Industrial applicability]

[0098] The present disclosure provides an additive for mushroom culture media that can reduce weighing errors and improve uniform mixing in the culture medium. The mushroom culture media additive of the present disclosure can be used for culturing various mushroom species. In one embodiment, the mushroom culture media additive of the present disclosure can be used for culturing shiitake, hollyhock, enoki mushroom, buna shimeji, nameko, maitake, king oyster mushroom, lion's mane mushroom, or oyster mushroom. [Explanation of Symbols]

[0099] 1. Mushroom culture medium manufacturing system 2. Bulking agent tank 3 Antacid tank 4a 1st stirring tank 4b 2nd stirring tank 5a First stirring shaft 5b Second stirring shaft 6a First stirring blade 6b Second stirring blade 7 Culture medium raw material tank 8. Sprinkler system 9. Mushroom culture medium recovery container 10a First stirring device 10b Second stirring device

Claims

1. Contains a bulking agent and an antacid, The aforementioned antacid contains an aluminum compound, The dry weight ratio of the bulking agent to the antacid is 98:2 to 50:

50. An additive for mushroom culture media with an angle of repose of 50 degrees or less.

2. The mushroom culture substrate additive according to claim 1, wherein the bulking agent comprises one or more selected from the group consisting of sawdust, corn cob meal, bagasse, beet meal, okara, coffee grounds, soybean hulls, rice bran, wheat bran, and cotton hull.

3. The mushroom culture medium additive according to claim 1, wherein the antacid further comprises one or more selected from calcium compounds, magnesium compounds, and sodium compounds.

4. The additive for mushroom culture medium according to claim 1, wherein the content of aluminum atoms in the antacid is 60% by weight or less, based on the total weight of the antacid, in terms of oxides.

5. The mushroom culture substrate additive according to claim 3, wherein the calcium atom content in the antacid is 60% by weight or less, based on the total weight of the antacid, in terms of oxides.

6. The mushroom culture substrate additive according to claim 3, wherein the magnesium atom content in the antacid is 30% by weight or less, based on the total weight of the antacid, in terms of oxides.

7. A process of mixing a bulking agent and an antacid to obtain an additive for mushroom culture media, and The process includes the step of mixing the aforementioned mushroom culture medium additive with culture medium raw materials to obtain a mushroom culture medium, The aforementioned antacid contains an aluminum compound, The dry weight ratio of the bulking agent to the antacid is 98:2 to 50:

50. A method for producing a mushroom culture medium, wherein the angle of repose of the mushroom culture medium additive is 50 degrees or less.

Citation Information

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