System, method, and sorting device for calculating droplet delay time

The system and method using dual laser sources and detectors in flow cytometers accurately calculate droplet delay time, enhancing sorting efficiency by considering particle size and image analysis, addressing the limitations of traditional methods in glass pool-based systems.

JP7864718B2Active Publication Date: 2026-05-25BECKMAN COULTER BIOTECHNOLOGY (SUZHOU) CO LTD +1
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Patent Information

Authority / Receiving Office
JP Β· JP
Patent Type
Patents
Current Assignee / Owner
BECKMAN COULTER BIOTECHNOLOGY (SUZHOU) CO LTD
Filing Date
2021-12-28
Publication Date
2026-05-25

AI Technical Summary

Technical Problem

Existing sorting devices, such as flow cytometers, face challenges in accurately calculating droplet delay time, which is crucial for proper electrostatic deflection of droplets containing target particles, especially in glass pool-based systems where traditional methods based on image processing fail to account for fluctuations before the nozzle system.

Method used

A system and method utilizing two laser sources and detectors to measure the time a particle takes to pass through specific points in the liquid flow, allowing for the calculation of droplet delay time by determining first and second delay times, which can be averaged or adjusted based on particle size, and incorporating a camera for additional image-based calculations.

Benefits of technology

Enables precise calculation of droplet delay time in sorting devices, improving the accuracy of droplet deflection and sorting efficiency, particularly in glass pool-based flow cytometers, by accounting for particle size and using multiple detection points and image analysis.

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Abstract

The present application discloses a system and method for calculating a droplet delay time in a liquid flow of a sorting device and a sorting device, the system including a first laser source configured to emit a first laser beam into the liquid flow of the sorting device, where the first laser beam and the liquid flow intersect at a first laser reference point located in a nozzle system of the sorting device, a second laser source configured to emit a second laser beam into the liquid flow, where the second laser beam and the liquid flow intersect at a second laser reference point located outside the nozzle system, and a droplet delay time calculation unit configured to calculate the droplet delay time.
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