Target cells or cell screening method, and biological culture chip

Abstract

To provide a biological culture chip suitable even for complex detection of cell biological functions such as antibody secretion and growth factor secretion.SOLUTION: Provided is a biological culture chip including a matrix formed of a matrix material, and culture chambers formed on a surface of the matrix to define the culture chambers, the matrix material of the matrix having an equilibrium swelling ratio of 1.25 to 1.75 and the culture chambers being formed in openings configured on the surface of the matrix to define the culture chambers.SELECTED DRAWING: Figure 3

Claims

1. A kit for screening target cells, These target cells are suitable for secreting target molecules, This kit is, Hydrogel; Signaling molecules; and Includes biological culture chips, The biological culture chip is Matrix formed from matrix material; and The culture chamber is formed on an opening located on the surface of the matrix to define the culture chamber, and the culture chamber is configured to culture candidate single cells under conditions suitable for secreting the target molecule; Here, the hydrogel is positioned to form a signal screening layer within the culture chamber. The signal screening layer is intended to cover the candidate single cell, and the mechanical strength of the signal screening layer is lower than the mechanical strength of the matrix material. The signaling molecule is positioned to be incorporated into the signal screening layer to specifically recognize the target molecule, and the target molecule can bind to the signaling molecule; Here, the equilibrium swelling ratio of the matrix material is 1.25 to 1.75, kit.

2. The kit according to claim 1, wherein the solid-liquid phase transition temperature of the matrix material is 40 to 45°C.

3. The kit according to claim 1, wherein a recess is formed in the contact area between the matrix and the cells during cell proliferation.

4. The kit according to claim 3, wherein the depth of the recess is 5% or more of the diameter of the cell.

5. The kit according to claim 1, wherein the matrix material is selected from the group consisting of collagen hydrogel, methylcellulose, agarose gel, polyacrylamide gel, and mixtures thereof in at least two different proportions.

6. The kit according to claim 1, wherein the matrix further comprises cytokines corresponding to biocompatible materials.

7. The matrix consists of fetal bovine serum, protein A / G, collagen, gelatin, and bovine blood. The kit according to claim 1, further selected from the group consisting of clear albumin and mixtures of at least two thereof.

8. The kit according to claim 1, wherein the diameter of the opening of the culture chamber is smaller than the diameter of the bottom of the culture chamber.

9. The kit according to claim 1, wherein the diameter of the opening is 8 to 25 μm and the depth is 15 to 35 μm.

10. The kit according to claim 1, wherein the culture chamber is composed of a plurality of microwells, the microwells form a predetermined pattern, and the distance between two adjacent microwells is 10 to 100 μm.

11. The kit according to claim 10, further comprising a positioning marker formed on the matrix.

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