An immuno-janus particle (IJP) assay for exosome and virus detection

WO2024220497A3PCT designated stage expired Publication Date: 2024-12-26UNIV OF NOTRE DAME DU LAC +5
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Patent Information

Application Number
PCT/US2024/024930
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-04-18
Filing Date
2024-04-17
Publication Date
2024-12-26

AI Technical Summary

Technical Problem

Current exosome isolation technologies from plasma are inefficient, with low yield and purity, and lack high-throughput capabilities, making accurate quantification and profiling of exosomal protein markers labor-intensive and time-consuming.

Method used

A system utilizing an asymmetric nanopore membrane and magnetic or non-magnetic Janus particles conjugated to capture antibodies for isolating exosomes and detecting colocalized proteins, achieving high yield and purity with fluid crossflow through the nanopore membrane, and using image recording devices to measure rotational frequency changes.

Benefits of technology

The system achieves an isolated exosome yield of at least 85% with high sensitivity and specificity, enabling rapid profiling of exosomal proteins and potential cancer marker detection in under 60 minutes.

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Abstract

Described herein are systems, methods, and devices for isolating exosomes and detecting colocalized exosomal proteins. In some embodiments, the disclosed systems, methods, and devices may comprise asymmetric nanopore membranes, magnetic or non-magnetic Janus particles, and capture and detector antibodies.
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Citation Information

Patent Citations

  • Force Mediated Assays

    US20120288852A1

  • Size-based asymmetric nanopore membrane (ANM) filtration for high-efficiency exosome isolation, concentration, and fractionation

    US20220347686A1