Long-distance transport box and transport method for hermetia illucens eggs and larvae

Abstract

Provided are a long-distance transport box and transport method for the long-distance transport of Hermetia illucens eggs and larvae. The long-distance transport box comprises: a box body (1) and a box lid (2), wherein several thermally insulating assemblies (3) are provided in the box body (1); each thermally insulating assembly (3) comprises a sealed bag (31) and a phase-change material filling the sealed bag (31); and the several thermally insulating assemblies (3) enclose, in the box body (1), an accommodating space configured for the placement of an egg box. By means of the transport box, the hatching rate of Hermetia illucens eggs can increase, and the loss of Hermetia illucens eggs and larvae during transport is reduced.

Description

Long-distance transport box and transport method for black soldier fly eggs and larvae Technical Field

[0001] The present application relates to the field of transportation of black soldier fly eggs and larvae, and in particular to a long-distance transportation box for black soldier fly eggs and larvae and a transportation method thereof. Background Art

[0002] The black soldier fly is a saprophytic insect of the family Schizontidae that feeds on livestock and poultry manure and domestic waste to produce high-value animal protein feed. Due to its rapid reproduction, large biomass, wide-ranging diet, high absorption and conversion rate, easy management, low breeding costs, and good palatability for animals, it is utilized as a resource insect, becoming a resource insect on par with maggots, mealworms, and barley worms. It has been promoted worldwide and is widely used to treat waste such as chicken manure, pig manure, and kitchen waste.

[0003] Currently, the demand for black soldier flies in production and daily life is extremely high. To meet this demand, black soldier flies are primarily farmed in centralized facilities and then transported nationwide. Black soldier flies are best used in the larval stage of domestic waste disposal. Since the larval stage lasts only 15 days, to maximize waste disposal efficiency, eggs are typically transported to their destination before hatching.

[0004] Currently, black soldier fly eggs are primarily transported in summer using cardboard boxes with ventilation holes or foam boxes with ice packs. In winter, foam boxes are often used. However, in hot and cold seasons, the eggs are subject to drastic temperature fluctuations, as cardboard boxes and foam boxes lack temperature control, resulting in some eggs dying and a reduced hatch rate. Furthermore, using a constant temperature system requires specialized vehicles with temperature-controlled compartments, which is restrictive and significantly increases costs.

[0005] Summary of the Invention

[0006] In order to improve the problem that black soldier fly eggs are easily killed by drastic temperature changes during the current transportation process, the present application provides a method for long-distance transportation of black soldier fly eggs and larvae.

[0007] The present application provides a box and method for long-distance transportation of black soldier fly eggs and larvae using the following technical solutions:

[0008] A transport box for long-distance transport of black soldier fly eggs and larvae, comprising a box body, a box cover, and several insulation components inside the box body;

[0009] The heat-insulating assembly includes a sealed bag filled with a phase-change material, wherein the phase-change material comprises, by weight, 73% to 75% of butyl stearate, 18% to 20% of ethylene butyl ester, 2% to 3% of tripalmitin, 2% to 3% of trimyristin, and 1% to 3% of 1,3-2,4-bis(3,4-dimethyl)benzylidene sorbitol; and a plurality of the heat-insulating assemblies are arranged in a transport box to form a storage space for placing insect egg boxes.

[0010] Preferably, the phase change temperature of the phase change material from solid to fluid is 17-22°C.

[0011] Preferably, a heat-insulating layer is provided on the inner wall of the box body and the inner wall of the box cover.

[0012] Preferably, the insulation layer includes an aluminum foil layer and an EPE layer, one side of the aluminum foil layer is adhered to the inner wall of the box body and the inner wall of the box cover, and the other side of the aluminum foil layer is adhered to the EPE layer.

[0013] Preferably, a sealing layer is provided on the outer wall of the box body, and the sealing layer is used to seal the gap between the box body and the box cover.

[0014] Preferably, the box body and the box cover are both made of foam material.

[0015] Preferably, the sealed bag is provided with a plurality of cylinders with closed ends, and the phase change material is filled in the cylinders and the gaps between the cylinders.

[0016] Preferably, the plurality of cylinders are divided into a plurality of rows, the axes of the cylinders in each row are located in the same plane, and the end surface of each cylinder is tangent to the end surfaces of two cylinders in the adjacent row.

[0017] Another object of the present application is to provide a method for long-distance transportation using the transport box for black soldier fly eggs and larvae, which comprises: placing black soldier fly eggs, larvae and bedding into an egg box, sealing the air holes on the egg box with breathable gauze, placing the egg box in the transport box, placing one of several insulation components above the egg box, then covering the box with a lid, and sealing the transport box for transportation.

[0018] By adopting the above technical solution, the problem that black soldier fly eggs are easily affected by changes in environmental temperature and their hatching rate is reduced can be improved. The phase change temperature range of the insulation board prepared by the ratio of the present application is between 17 and 22°C, and it has excellent heat insulation and heat preservation effects. It can maintain a stable temperature in the foam box, thereby protecting black soldier fly eggs and larvae. The insulation board is in a solid state in normal state, wherein 1,3-2,4-di(3,4-dimethyl)benzylidene sorbitol is used as a nucleating agent. The insulation board can insulate heat, reduce heat conduction between the foam box and the outside world, and keep the temperature in the foam box constant within a range suitable for black soldier fly eggs and larvae. When the temperature in the foam box exceeds the phase change temperature of the insulation board, the insulation board is converted into liquid and absorbs the heat in the foam box. At this time, the liquid insulation material forms a liquid bag in the sealing tape, which can still play an insulating role and still has a protective effect on black soldier fly eggs and larvae.

[0019] This application utilizes the thermal insulation and heat preservation properties of the foam material itself, and cooperates with the insulation board set on the inner wall of the foam box to greatly improve the thermal insulation and heat preservation performance of the foam box, so that the foam box has a better temperature control effect and delays the temperature change in the foam box. In addition, the padding in the egg box has good air permeability and buffers pressure, which can significantly improve the hatching rate of black soldier fly eggs.

[0020] Preferably, the bedding material is one or a combination of sawdust, wood shavings, straw, straw powder, puffed corn flour, alfalfa, and timothy grass.

[0021] Preferably, the bedding is a mixture of sawdust, rice straw powder and straw, the sawdust and rice straw powder are evenly mixed and used as the lower layer bedding, the weight percentage of sawdust in the lower layer bedding is 20% to 50%, the straw is spread on the mixture of sawdust and rice straw powder as the upper layer bedding, the upper layer bedding completely covers the lower layer bedding, and the black soldier fly eggs and larvae are mixed in the lower layer bedding.

[0022] Preferably, the weight ratio of sawdust to straw powder in the lower layer of bedding is 1:1.

[0023] The experiment showed that the use of bedding with this composition ratio is beneficial to improving the hatching rate of black soldier fly eggs.

[0024] Preferably, the weight of the black soldier fly eggs and larvae is 1.5 to 4 times the weight of the lower litter.

[0025] By adopting the above technical solution, the lower layer of bedding has a buffering and shock-absorbing effect on the black soldier fly eggs and larvae, which is beneficial to protecting the black soldier fly eggs and larvae. When the weight of the black soldier fly eggs and larvae exceeds 4 times the weight of the lower layer of bedding, the lower layer of bedding is too little relative to the black soldier fly eggs and larvae, and the buffering and shock-absorbing effect is reduced. In addition, if there are too many black soldier fly eggs and larvae in the foam box, the oxygen content will be consumed in large quantities, resulting in a lower hatching rate of the black soldier fly eggs.

[0026] Preferably, one or two layers of egg boxes arranged in a rectangular array are placed in the transport box, and the number of egg boxes on the bottom layer is denoted as M. Wherein [X] represents rounding, A is the length of the long side of the bottom surface of the transport box, A≤70cm, B is the length of the short side of the bottom surface of the transport box, B≤50cm, d is the thickness of the insulation board, 1cm≤d≤5cm, and the height of the internal space of the transport box is 22-25cm; when there are two layers of egg boxes in the transport box, the number of egg boxes on the upper layer is recorded as N, or And the upper egg box and the lower egg box are placed alternately.

[0027] Experiments have shown that controlling the placement of black soldier fly eggs and larvae in this way can not only transport the eggs and larvae according to the optimal economic benefits, but also reduce the loss of eggs during transportation.

[0028] In summary, this application includes at least one of the following beneficial technical effects:

[0029] 1. This application uses a foam box with good thermal insulation and heat preservation properties as a transport container, and an insulation board is arranged on the inner wall of the foam box, which greatly improves the thermal insulation and heat preservation performance of the foam box, so that the foam box has a better temperature control effect and can delay the temperature change in the foam box. Combined with the good air permeability and pressure buffering characteristics of the bedding in the egg box, it can significantly improve the hatching rate of black soldier fly eggs.

[0030] 2. By controlling the placement amount of black soldier fly eggs and larvae, the present application can not only transport the eggs and larvae in accordance with the optimal economic benefits, but also reduce the loss of eggs and larvae during transportation.

[0031] 3. The transport box of the present application is designed based on the transport method of the present application and can be used to replace traditional cardboard boxes to solve the problem that insect eggs and larvae may die due to drastic temperature changes during transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] FIG1 is a schematic diagram of the placement of the egg box in Example 1 of the present application;

[0033] Figure 2 is an overall schematic diagram of the transport box embodiment of the present application.

[0034] FIG3 is a cross-sectional schematic diagram of a transport box according to an embodiment of the present application;

[0035] FIG4 is an enlarged schematic diagram of portion A in FIG3 ;

[0036] FIG5 is a schematic cross-sectional view of a heat preservation assembly according to an embodiment of the present application when placed flat;

[0037] FIG6 is an exploded schematic diagram of a transport box according to an embodiment of the present application;

[0038] FIG7 is a schematic structural diagram of a transport box cover according to an embodiment of the present application;

[0039] Explanation of the accompanying reference numerals: 1. Box body; 11. Recess; 2. Box cover; 21. Raised edge; 3. Insulation component; 31. Sealing bag; 32. Dispersing piece; 321. Cylinder; 4. Sealing layer; 5. Insulation layer; 51. Aluminum foil layer; 52. Pearl cotton layer. DETAILED DESCRIPTION

[0040] In order to facilitate understanding of the technical solution of the present application, the present application is further described in detail below in conjunction with tables and examples, but they are not intended to limit the scope of protection of the present application.

[0041] An embodiment of the present application provides a transport box for long-distance transportation of black soldier fly eggs and larvae, and the transport box is further described in detail with reference to the accompanying drawings.

[0042] Referring to FIG2 , the transport box for black soldier fly eggs and larvae includes a box body 1 and a box lid 2. Both the box body 1 and the box lid 2 are made of a foam material. The foam may be polystyrene foam, polyurethane foam, phenolic foam, EPS foam, XPS foam, or the like, which is not specifically limited in this embodiment. The foam material has good thermal insulation and heat preservation effects, which can improve the thermal insulation and heat preservation effects of the transport box and avoid drastic temperature fluctuations within the transport box. The foam material also has good cushioning and shock absorption capabilities, which can reduce the vibrations experienced by the black soldier fly eggs and larvae during transportation, thereby improving the survival rate of the black soldier fly eggs and larvae.

[0043] 3 and 4 , an insulation layer 5 is bonded to the inner wall of the box body 1 and the inner wall of the box lid 2. The insulation layer 5 is composed of an aluminum foil layer 51 and an EPP layer 52. The aluminum foil layer 51 is attached to the inner wall of the box body 1 and the inner wall of the box lid 2, and the EPP layer 52 is attached to the other side of the aluminum foil layer 51. Both the aluminum foil and the EPP have good thermal insulation effects, which are conducive to improving the survival rate of black soldier fly eggs and larvae. The EPP layer 52 also has a certain shock absorption capacity, which can further reduce the vibration experienced by the black soldier fly eggs and larvae during transportation, thereby promoting the survival of the black soldier fly eggs and larvae.

[0044] Referring to Figures 3 to 5 , the box 1 is further provided with several insulation components 3, which are located inside the insulation layer 5. The insulation components 3 are composed of a sealed bag 31, a dispersion member 32 placed inside the sealed bag, and a phase change material filled inside the dispersion member 32 and between the sealed bag 31 and the dispersion member 32. When the external temperature is higher than that of the phase change material, the phase change material absorbs heat and changes to a fluid state, thereby reducing the transfer of high temperature from the external environment to the foam box and providing thermal insulation. When the external temperature is lower than that of the phase change material, the phase change material becomes solid, providing thermal insulation and protection from the cold.

[0045] In this embodiment, six insulation assemblies 3 are provided, forming a rectangular storage space. During transportation, black soldier fly eggs are placed in the storage space formed by the insulation assemblies 3. Due to the thermal insulation properties of the insulation assemblies 3, the storage space does not experience significant temperature fluctuations, resulting in a good temperature control effect. This can maintain a stable temperature within the transport box, which is beneficial for improving the survival rate of black soldier fly eggs and larvae and the hatching rate. In other embodiments, the number of insulation assemblies 3 corresponding to each of the six sides of the storage space can be more than two to achieve a better temperature control effect.

[0046] In this embodiment, the phase change material is made of a mixture of butyl stearate, ethylene butyl ester, tripalmitin, trimyristin, and 1,3-2,4-di(3,4-dimethyl)benzylidene sorbitol. The phase change temperature range is 17 to 22°C, which is conducive to maintaining the temperature inside the transport box at a suitable temperature for the survival of black soldier fly eggs and larvae.

[0047] Referring to Figure 5 , the disperser 32 is composed of a number of closed-end cylinders 321, which are joined in sequence. The end faces of the cylinders 321 are tangential to each other, and the interiors of the cylinders 321 and the gaps between them are filled with a phase-change material. The cylinders 321 are arranged in multiple groups, with the axes of the cylinders 321 in each group lying in the same plane. The end faces of each cylinder 321 are tangential to the end faces of two cylinders 321 in the adjacent group. In other words, the cylinders 321 in adjacent groups are staggered, forming a three-circle tangential connection between the cylinders 321, which contributes to a more stable structure for the disperser 32.

[0048] The number of cylinders 321 included in the dispersion element 32 is determined based on the total volume of all cylinders 321 and the total volume of the gaps between cylinders 321 approaching the volume of the sealed bag 31 when it is fully expanded. This provides support for the sealed bag 31 and allows the external structure of the insulation component 3 to approach the external contour of the dispersion element 32. This allows the insulation component 3 to maintain a relatively fixed contour during phase change in the phase change material, thereby facilitating a more stable thermal insulation effect. Because the cylinders 321 are closed at both ends, the phase change process of the phase change material within the cylinders 321 is fixed within a specific area, preventing the phase change material from concentrating in a single area and reducing the temperature control area. This allows the insulation component 3 to maintain a temperature control area sufficient to cover the egg box. The phase change material within the gaps between the cylinders 321 allows the temperature control surface of the insulation component 3 to be as complete as possible, thereby improving the temperature control effect.

[0049] Referring to Figures 6 and 7, a notch 11 is provided at the top of the housing 1, near the inner wall, along the outline of the opening of the housing 1. A ridge 21 is provided on the inner wall of the lid 2, which fits snugly within the notch 11. When the lid 2 is mated with the housing 1, the ridge 21 fits snugly within the notch 11, thereby enhancing the secure fit and sealing effect between the housing 1 and the lid 2, reducing heat transfer, and improving the survival rate of black soldier fly eggs and larvae.

[0050] Referring to Figure 2 , a sealing layer 4 is also attached to the outer wall of the box body 1. The gap between the box body 1 and the box cover 2 is sealed by the sealing layer 4, keeping the transport box sealed. This further reduces the amplitude of internal temperature fluctuations, which is beneficial for improving the survival rate of black soldier fly eggs and larvae. It also ensures that the box body 1 and the box cover 2 are firmly connected, preventing the box cover 2 from loosening during transportation. The sealing layer 4 can be made of tape, sealant, or other viscous material that can achieve a sealing function, and this application does not impose specific limitations on this.

[0051] The implementation principle of a black soldier fly egg and larvae transport box in an embodiment of the present application is as follows: the present application utilizes a phase change material filled in a sealing bag 31 and disperses the phase change material through a dispersion piece 32 to maintain the temperature of the space where the egg box is located as stable as possible; when the external temperature is higher than the temperature of the phase change material, the phase change material absorbs heat and changes into a fluid state, thereby reducing the transfer of external high temperature into the foam box and playing a role of heat insulation; when the external temperature is lower than the temperature of the phase change material, the phase change material is in a solid state, thereby playing a role of heat preservation and cold protection; the temperature inside the transport box is controlled by phase change of the phase change material, which reduces the amplitude of temperature change inside the transport box, and is beneficial to improving the survival rate of black soldier fly eggs and larvae and improving the hatching rate.

[0052] The following describes in detail the methods for long-distance transportation of eggs and larvae:

[0053] Example 1

[0054] Methods for long-distance transportation of black soldier fly eggs:

[0055] Black soldier fly eggs and bedding are placed in an egg box, and the air holes on the egg box are sealed with breathable gauze to prevent leakage of the bedding or black soldier fly eggs. In this embodiment, the bedding is straw, and the weight of the black soldier fly eggs in each egg box is 35g. The straw is spread on top of the black soldier fly eggs and completely covers the black soldier fly eggs.

[0056] Place insulation boards on the five inner walls of the foam box, and place the insulation boards in a sealed plastic bag. Place the egg boxes in the foam box. Place two layers of egg boxes in the foam box. The upper and lower layers of egg boxes are arranged in a rectangular array. The number of egg boxes on the bottom layer is recorded as M. The number of upper egg boxes is recorded as N. or Wherein, [X] represents rounding, A is the length of the long side of the inner bottom surface of the foam box, A≤70cm, B is the length of the short side of the inner bottom surface of the foam box, B≤50cm, d is the thickness of the insulation board, 1cm≤d≤5cm, and the height of the internal space of the foam box is 22~25cm.

[0057] In this embodiment, A is 37 cm, B is 27 cm, d is 1.5 cm, the height of the internal space of the foam box is 22 cm, M is 6, N is 3, the diameter of the egg box is 8 cm, and the height is 7 cm. The upper egg box and the lower egg box are placed alternately, as shown in Figure 1. The lower egg box is arranged in a 2*3 matrix, and the upper egg box is arranged in a 1*3 matrix.

[0058] Then, an insulation board is placed above the egg box. The insulation board is placed in a sealed plastic bag. The insulation board on the inner wall of the foam box and the insulation board above the egg box are successively abutted against each other to form a sealed accommodating space. The egg box is located in the accommodating space.

[0059] After placing the egg box, close the lid, seal the gap on the foam box lid with tape, and then transport it.

[0060] In this embodiment, the material of the insulation board is composed of 73% butyl stearate, 20% ethylene butyl ester, 3% tripalmitin, 3% trimyristin, and 1% 1,3-2,4-bis(3,4-dimethyl)benzylidene sorbitol by weight.

[0061] The preparation method of the insulation board is as follows: butyl stearate, ethylene butyl ester, tripalmitin, and trimyristin are mixed and heated to 85±5°C, stirred for 1 hour until the mixture is uniformly mixed, and then 1,3-2,4-di(3,4-dimethyl)benzylidene sorbitol is added, and stirring is continued for 30 minutes. The mixture is poured into a mold, cooled and formed, and then demolded.

[0062] The phase change temperature of the prepared insulation board is 17-22°C. When used, it can maintain the temperature inside the foam box at 17-22°C. When the temperature inside the foam box exceeds 22°C, the insulation board changes from solid to liquid, but will not spill out of the sealed plastic bag. The insulation material converted into fluid can still play a role in heat insulation and continue to protect black soldier fly eggs.

[0063] Furthermore, based on the above transportation method and the material and performance of the insulation board, the present application provides a transport box implementation scheme for implementing the above-mentioned long-distance transportation method of black soldier fly eggs, the transport box includes a box body and a box cover, and several insulation components are arranged in the box body, the insulation components include a sealed bag and a phase change material filled inside the sealed bag (that is, the insulation board mentioned above), and the several insulation components form a storage space in the box body for placing the egg box.

[0064] Preferably, a heat-insulating layer is provided on the inner wall of the box body and the inner wall of the box cover.

[0065] Preferably, the insulation layer is composed of an aluminum foil layer and an EPE layer, one side of the aluminum foil layer is adhered to the inner wall of the box body and the inner wall of the box cover, and the EPE layer is adhered to the other side of the aluminum foil layer.

[0066] Preferably, a sealing layer is provided on the outer wall of the box body, and the sealing layer is used to seal the gap between the box body and the box cover.

[0067] Preferably, the box body and the box cover are both made of foam material.

[0068] Preferably, a dispersion piece is provided in the sealed bag, and the dispersion piece is composed of a number of cylinders with closed ends connected in sequence, the end faces of the cylinders are tangent to each other in sequence, and the phase change material is filled in the cylinders and the gaps between the cylinders.

[0069] Preferably, the plurality of cylinders are divided into a plurality of rows, the axes of the cylinders in each row are located in the same plane, and the end surface of each cylinder is tangent to the end surfaces of two cylinders in an adjacent row.

[0070] It should be noted that in the examples and comparative examples of the present application, the bedding includes powdered bedding and straw-shaped bedding according to actual selection. When the bedding includes only powdered bedding, the powdered bedding and black soldier fly eggs are mixed evenly and the total weight is controlled to be 50g. When the bedding includes only straw-shaped bedding, 35g of black soldier fly eggs are placed in each egg box, and the straw-shaped bedding is spread over the black soldier fly eggs and completely covers the black soldier fly eggs. When the bedding includes both powdered bedding and straw-shaped bedding, the sum of the weight of the black soldier fly eggs and the powdered bedding in each egg box is controlled to be 50g and mixed evenly, and the straw-shaped bedding covers the mixture of the black soldier fly eggs and the powdered bedding.

[0071] It should be noted that in the embodiments and comparative examples of the present application, rice straw, alfalfa, and timothy grass are straw-like bedding materials, sawdust, rice straw powder, puffed corn flour, bran, and wood shavings are powdered bedding materials, and puffed corn flour refers to a starch gelatinized product of corn under the combined effects of moisture, heat, mechanical shearing, friction, kneading, and pressure difference. It has a light yellow appearance and a fluffy texture. Puffed corn flour is common knowledge and will not be described in detail in this embodiment.

[0072] Example 2

[0073] The difference from Example 1 is that the bedding material is puffed corn flour, the weight of the black soldier fly eggs in the egg box is 35 g, the weight of the puffed corn flour is 15 g, and the puffed corn flour and the black soldier fly eggs are mixed evenly.

[0074] Example 3

[0075] The difference from Example 1 is that the bedding is rice straw powder, the rice straw powder and the black soldier fly eggs are evenly mixed, the weight of the black soldier fly eggs in the egg box is 35g, the weight of the rice straw powder is 15g, and the rice straw powder and the black soldier fly eggs are evenly mixed.

[0076] Example 4

[0077] The difference from Example 1 is that the bedding is bran, the weight of the black soldier fly eggs in the egg box is 35 g, the weight of the bran is 15 g, and the bran and the black soldier fly eggs are evenly mixed.

[0078] Example 5

[0079] The difference from Example 1 is that the bedding is wood chips, the weight of the black soldier fly eggs in the egg box is 35g, the weight of the wood chips is 15g, and the wood chips and black soldier fly eggs are mixed evenly.

[0080] Example 6

[0081] The difference from Example 1 is that the bedding material is wood shavings, the weight of the black soldier fly eggs in the egg box is 35 g, the weight of the wood shavings is 15 g, and the wood shavings and the black soldier fly eggs are mixed evenly.

[0082] Example 7

[0083] The difference from Example 1 is that alfalfa is used as the bedding, the weight of the black soldier fly eggs in the egg box is 35 g, and the alfalfa covers the black soldier fly eggs.

[0084] Example 8

[0085] The difference from Example 1 is that the bedding material is timothy grass, the weight of the black soldier fly eggs in the egg box is 35 g, and the timothy grass covers the black soldier fly eggs.

[0086] With reference to Examples 1-8, Examples 9-16 are respectively provided. The only difference between Examples 9-16 and Examples 1-8 is that the material of the insulation board is composed of 75% butyl stearate, 18% ethylene butyl ester, 2% tripalmitin, 2% trimyristin, and 3% 1,3-2,4-di(3,4-dimethyl)benzylidene sorbitol by weight.

[0087] Verification test

[0088] With Example 1-8 as a reference, Comparative Example 1-8 is set. The only difference between Comparative Example 1-8 and Example 1-8 is that cardboard boxes with holes are used for summer transportation, and foam boxes are used for winter transportation, and there is no insulation board inside the foam boxes.

[0089] With reference to Example 1-8, comparative examples 9-16 were set. The only difference between comparative examples 9-16 and Example 1-8 was that foam boxes were used for summer transportation, ice packs were placed in the foam boxes, and no winter test was performed.

[0090] Examples 1-16, Comparative Examples 1-8, and Comparative Example A were respectively transported for 3 days in summer (August) and winter (December), and Comparative Examples 9-16 were only transported for 3 days in summer (August). The transportation test was carried out with Nanning, Guangxi as the departure point and Zunyi, Guizhou as the destination. Three parallel tests were conducted for each example and comparative example.

[0091] Comparative Example A is set up, and the only difference between Comparative Example A and Example 1 is that the material of the insulation board is composed of 62% butyl stearate, 18% ethylene butyl ester, 8% tripalmitin, 9% trimyristin, and 3% 1,3-2,4-bis(3,4-dimethyl)benzylidene sorbitol by weight.

[0092] Examples 1-16 and Comparative Examples 1-16 and Comparative Example A were all tested simultaneously, i.e., they were transported on the same vehicle at the same time. The temperature range during the entire test process was: 26-40°C in summer and -2-8°C in winter.

[0093] Hatchability calculation:

[0094] Determination of the number of black soldier fly eggs per gram: Weigh 30 different portions of eggs, 2 mg each (accurate to 0.1 mg), place them in a culture dish, add alcohol to soak and observe, if there is no overlap, take pictures and count them, thus estimating that the number of black soldier fly eggs per gram is about 40,000 / g.

[0095] After the eggs were transported to their destination, one box of black soldier fly eggs containing litter from each replicate was placed in separate large plastic boxes and fed with chicken feed. The eggs were then incubated for seven days in a constant temperature and humidity environment (28°C and 65%). After the incubation period, the boxes were removed, the larvae were counted, and the hatch rate was calculated as follows: hatch rate = number of larvae hatched per box / (weight of eggs per box × number of eggs per gram) × 100%. Larvae were then fed with food waste and other materials for 10 days before being harvested and counted as the commercial number. Larval survival rate = commercial number of larvae / number of larvae hatched at the end of incubation × 100%. See Table 1 for detailed data.

[0096] Table 1: Litter composition and black soldier fly egg hatching rate and larval survival rate in Examples 1-16, Comparative Examples 1-16 and Comparative Example A

[0097] Combining Examples 1-16, Comparative Examples 1-16, and the data in Table 1, it can be seen that the transportation method of the present application can significantly improve the hatching rate of black soldier fly eggs and the survival rate of larvae. Compared with the method of using perforated cardboard boxes and placing ice packs inside foam boxes in the summer, and the method of using ordinary foam boxes in the winter, the hatching rate of black soldier fly eggs and the survival rate of larvae in the examples of the present application are significantly improved. This shows that the insulation board has a good temperature control effect in both summer and winter, which helps to stabilize the temperature inside the foam box, thereby improving the hatching rate of black soldier fly eggs and the survival rate of larvae.

[0098] By comparing the data in Examples 1-16, it can be seen that the hatching rate of black soldier fly eggs and the survival rate of larvae in Examples 9-16 are slightly higher than the hatching rate of black soldier fly eggs in Examples 1-8, indicating that the use of the insulation board with the material ratio in Examples 9-16 is more conducive to improving the hatching rate of black soldier fly eggs and the survival rate of larvae.

[0099] By comparing the data of Example 1, Example 9 and Comparative Example A, it can be seen that when the ratio of the various components of the material of the insulation board is inappropriate (Comparative Example A), the hatching rate of black soldier fly eggs and the survival rate of larvae are poor.

[0100] Example 17

[0101] The difference from Example 9 is that the bedding is a mixture of bran and sawdust, the weight ratio of bran to sawdust is 1:1, the weight of black soldier fly eggs in the egg box is 35g, the weight of the bedding is 15g, and the bedding and black soldier fly eggs are mixed evenly.

[0102] Example 18

[0103] The difference from Example 9 is that the bedding is a mixture of bran and straw powder, the weight ratio of bran to straw powder is 1:1, the weight of black soldier fly eggs in the egg box is 35g, the weight of the bedding is 15g, and the bedding and black soldier fly eggs are mixed evenly.

[0104] Example 19

[0105] The difference from Example 9 is that the bedding is a mixture of sawdust and straw powder, the weight ratio of sawdust to straw powder is 1:1, the weight of black soldier fly eggs in the egg box is 35g, the weight of the bedding is 15g, and the bedding and black soldier fly eggs are mixed evenly.

[0106] Example 20

[0107] The difference from Example 9 is that the bedding is made of bran and straw, the weight of the black soldier fly eggs in the egg box is 35g, the weight of the bran is 15g, the bran and the black soldier fly eggs are mixed evenly, and the straw is covered on top of the mixture of bran and black soldier fly eggs.

[0108] Example 21

[0109] The difference from Example 9 is that the bedding is made of sawdust and straw, the weight of black soldier fly eggs in the egg box is 35g, the weight of sawdust is 15g, the sawdust and black soldier fly eggs are mixed evenly, and the straw is covered on top of the mixture of sawdust and black soldier fly eggs.

[0110] Example 22

[0111] The difference from Example 9 is that the bedding uses sawdust, rice straw powder, and rice straw, and a mixture of sawdust and rice straw powder is used as the lower bedding, the weight ratio of sawdust to rice straw powder is 1:1, the weight of black soldier fly eggs in the egg box is 35 g, the weight of the lower bedding is 15 g, the lower bedding and the black soldier fly eggs are mixed evenly, and the rice straw is covered on top of the mixture of the lower bedding and the black soldier fly eggs.

[0112] Example 23

[0113] The difference from Example 9 is that the bedding uses sawdust, straw powder, and alfalfa, and a mixture of sawdust and straw powder is used as the lower bedding, the weight ratio of sawdust to straw powder is 1:1, the weight of black soldier fly eggs in the egg box is 35 g, the weight of the lower bedding is 15 g, the lower bedding and the black soldier fly eggs are evenly mixed, and alfalfa is covered on top of the mixture of the lower bedding and the black soldier fly eggs.

[0114] Control groups 1-5 were set, control group 1 was consistent with Example 4, control group 2 was consistent with Example 5, control group 3 was consistent with Example 3, control group 4 was consistent with Example 1, and control group 5 was consistent with Example 7.

[0115] The control groups 1-5 and Examples 17-23 were tested according to the test method and test conditions of the above-mentioned verification test. Three parallel tests were conducted for each example and control group. The specific data are shown in Table 2.

[0116] Table 2: Litter composition and black soldier fly egg hatching rate in control groups 1-5 and examples 17-23

[0117] Combining the data of controls 1-5, Examples 17-23 and Table 2, it can be seen that the selection of different bedding materials also affects the hatching rate of black soldier fly eggs. Compared with the use of a single type of bedding material, the use of a mixture of different types of bedding materials is beneficial to improving the hatching rate of black soldier fly eggs. Among them, the bedding material with the ratio in Example 22 is better. This is because sawdust and rice straw powder are powdered bedding materials that can be fully mixed with black soldier fly eggs, reducing the problem of water loss in eggs, which is beneficial to improving the hatching rate of eggs. Rice straw also has a certain thermal insulation effect, which can effectively prevent the eggs from freezing or being heated.

[0118] Example 24

[0119] The difference from Example 22 is that the weight percentage of wood chips in the lower layer of bedding is 20%.

[0120] Example 25

[0121] The difference from Example 22 is that the weight percentage of wood chips in the lower layer of bedding is 40%.

[0122] Example 26

[0123] The difference from Example 22 is that the weight percentage of wood chips in the lower layer of bedding is 60%.

[0124] A control group 6 was set up, and the control group 6 was consistent with Example 22.

[0125] The control group 6 and Examples 24-26 were tested according to the test method and test conditions of the above-mentioned verification test. Three parallel tests were conducted on each control group and example. The specific data are shown in Table 3.

[0126] Table 3: Litter ratios and black soldier fly egg hatching rates in control group 6 and examples 24-26

[0127] Sawdust is light and fluffier, which can reduce frictional heat between insect eggs, while straw powder has a certain weight and contains certain beneficial microorganisms, which can improve the thermal tolerance of black soldier fly eggs; in winter, both sawdust and straw powder have a heat-insulating effect, which can reduce the cold damage to insect eggs caused by low temperatures, and the beneficial microorganisms can increase the biological activity of insect eggs during the breeding process.

[0128] It can be seen from the data in Table 3 that when the weight ratio of sawdust to rice straw powder is 1:1, it can not only effectively inhibit the hatching of black soldier fly eggs during transportation, but also reduce the loss of water from the black soldier fly eggs, thereby improving the hatching rate of the black soldier fly eggs. Therefore, the weight ratio of sawdust to rice straw powder in the lower layer bedding of Example 22 is better.

[0129] Example 27

[0130] The difference from Example 22 is that the weight of the black soldier fly eggs in the egg box is 30 g, the weight of the lower layer bedding is 20 g, and the weight ratio of the black soldier fly eggs to the lower layer bedding is 1.5:1.

[0131] Example 28

[0132] The difference from Example 22 is that the weight of the black soldier fly eggs in the egg box is 33.3 g, the weight of the lower layer bedding is 16.7 g, and the weight ratio of the black soldier fly eggs to the lower layer bedding is approximately 2:1.

[0133] Example 29

[0134] The difference from Example 22 is that the weight of the black soldier fly eggs in the egg box is 38.5 g, the weight of the lower layer bedding is 11.5 g, and the weight ratio of the black soldier fly eggs to the lower layer bedding is about 3.3:1.

[0135] Example 30

[0136] The difference from Example 22 is that the weight of the black soldier fly eggs in the egg box is 40 g, the weight of the lower layer bedding is 10 g, and the weight ratio of the black soldier fly eggs to the lower layer bedding is 4:1.

[0137] Example 31

[0138] The difference from Example 22 is that the weight of the black soldier fly eggs in the egg box is 42 g, the weight of the lower layer bedding is 8 g, and the weight ratio of the black soldier fly eggs to the lower layer bedding is 5.25:1.

[0139] A control group 7 was set up, and the control group 7 was consistent with Example 22.

[0140] The control group 7 and Examples 27-31 were tested according to the test method and test conditions of the above-mentioned verification test. Three parallel tests were conducted on each control group and example. The specific data are shown in Table 4.

[0141] Table 4: Litter ratios and black soldier fly egg hatching rates in control group 7 and Examples 27-31

[0142] Combining the data from Control Group 7, Examples 27-31, and Table 4, it can be seen that when a mixture of sawdust and straw powder is used as the lower bedding material in a ratio of 1:1 and straw is used as the upper bedding material, the weight ratio of black soldier fly eggs to the lower bedding material affects the hatchability of the eggs. As the weight ratio of black soldier fly eggs to the lower bedding material increases, the hatchability of the eggs increases and then decreases. This is because the powdered bedding material in the lower layer provides a cushioning and shock-absorbing effect on the eggs. When the amount of black soldier fly eggs increases and the amount of lower bedding material decreases, the cushioning and shock-absorbing effect on the eggs decreases, resulting in a decrease in the hatchability of the eggs. However, when the amount of black soldier fly eggs is too small and the amount of the lower litter is too much, the black soldier fly eggs will be squeezed to a certain extent, hindering the air flow between the material and the black soldier fly eggs, thereby reducing the hatching rate of the black soldier fly eggs.

[0143] When the weight of black soldier fly eggs exceeds 4 times the weight of the lower layer of litter, the hatching rate of black soldier fly eggs is significantly reduced. This is because the lower layer of litter is too little relative to the black soldier fly eggs, the cushioning and shock absorption effect is reduced, and there are too many black soldier fly eggs in the foam box, the friction between the eggs increases, resulting in a lower hatching rate of black soldier fly eggs, causing the hatching rate of black soldier fly eggs to decrease.

[0144] From the data in Table 4, it can be seen that the weight ratio of eggs to lower layer litter in Example 29 of about 3.3:1 is more effective in improving the hatching rate of black soldier fly eggs.

[0145] Comparative Example 17

[0146] The difference from Example 29 is that cardboard boxes with holes are used for summer transportation, and foam boxes without insulation boards are used for winter transportation.

[0147] Comparative Example 18

[0148] The difference from Example 29 is that the black soldier fly egg box does not have bedding.

[0149] A control group 8 was set up, and the control group 8 was consistent with Example 29.

[0150] The control group 8 and comparative examples 17-18 were tested according to the test method and test conditions of the above-mentioned verification test. Three parallel tests were conducted on each control group and comparative example. The specific data are shown in Table 5.

[0151] Table 5: Hatching rate of black soldier fly eggs in control group 8 and comparative examples 17-18

[0152] Combining the data of control group 8, comparative examples 17-18 and Table 5, it can be seen that only the weight ratio of black soldier fly eggs to bedding or the bedding ratio in Example 29 has limited effect on improving the hatching rate of black soldier fly eggs. It can be seen that there is a synergistic effect between the weight ratio of black soldier fly eggs to bedding and the bedding ratio in Example 29 and the foam box with an insulation board in the embodiment of the present application. The combination of the two can greatly improve the hatching rate of black soldier fly eggs.

[0153] Example 30

[0154] The difference from Example 29 is that in the foam box, 6 egg boxes are placed in the lower layer, arranged in a 2*3 matrix, and 4 egg boxes are placed in the upper layer, arranged in a 2*2 matrix, and the upper and lower egg boxes are placed alternately.

[0155] A control group 9 was set up, which was consistent with Example 29.

[0156] Three parallel tests were conducted on Control Group 9 and Example 30 according to the above-mentioned verification test method. The specific data are shown in Table 6.

[0157] Table 6: Hatching rate of black soldier fly eggs in control group 9 and Example 30

[0158] As can be seen from the data of Table 6, the hatching rate of black soldier fly eggs in Example 30 is lower than the hatching rate of black soldier fly eggs in control group 9. The reason is that the quantity of egg boxes in Example 30 is greater than that in control group 9. In a limited space, in conjunction with the size of the egg box and the environment in which the eggs are placed, when the quantity of the egg box is less, the oxygen content in the foam box consumes less, which is better for the transportation of the eggs. However, from the perspective of economic benefit, the less the quantity of each box of foam box is placed, the higher the production cost is, which does not meet the actual demand of production. Therefore, according to the placement density control of the egg box of the present application, the upper layer egg box and the lower layer egg box are staggered, and this placement can both meet the demand of the egg living environment and can also be transported according to the maximum economic benefit.

[0159] In summary, the use of the transportation method of the present application to transport black soldier fly eggs can greatly improve the hatching rate of black soldier fly eggs. By using a foam box with insulation material and a specific bedding ratio to work together, and controlling the number of egg boxes placed in the foam box, the impact of different regions and climates on the black soldier fly eggs during transportation is reduced, making the black soldier fly eggs less susceptible to drastic temperature changes during transportation, and has good application prospects.

[0160] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A transport box for long-distance transport of black soldier fly eggs and larvae, comprising a box body, a box cover and a plurality of heat-insulating components in the box body; wherein the heat-insulating components comprise a sealed bag filled with a phase-change material, wherein the phase-change material comprises, by weight percentage, 73% to 75% of butyl stearate, 18% to 20% of ethylene butyl ester, 2% to 3% of tripalmitin, 2% to 3% of trimyristin, and 1% to 3% of 1,3-2,4-di(3,4-dimethyl)benzylidene sorbitol, and a plurality of the heat-insulating components are arranged in the transport box to form a containing space for placing an egg box.

2. The transport box according to claim 1, wherein the phase change temperature of the phase change material from solid to fluid is 17-22°C.

3. The transport box according to claim 1, wherein a heat-insulating layer is provided on the inner wall of the box body and the inner wall of the box cover.

4. The transport box according to claim 3, wherein the insulation layer comprises an aluminum foil layer and an pearl cotton layer, one side of the aluminum foil layer is adhered to the inner wall of the box body and the inner wall of the box cover, and the other side of the aluminum foil layer (51) is adhered to the pearl cotton layer.

5. The transport box according to claim 1, wherein a sealing layer is provided on the outer wall of the box body, and the sealing layer (4) is used to seal the gap between the box body and the box cover.

6. The transport box according to claim 1, wherein the box body (1) and the box cover (2) are both made of foam material.

7. The transport box according to claim 1, wherein the sealed bag is provided with a plurality of cylinders with closed ends, and the phase change material is filled in the cylinders and in the gaps between the cylinders (321).

8. The transport box according to claim 7, wherein the plurality of cylinders are divided into a plurality of rows, and the axes of the cylinders in each row are located in the same plane.

9. A method for long-distance transportation of black soldier fly eggs and larvae using the transport box of claim 1, comprising: The eggs, larvae and litter of black soldier flies are placed in an egg box, the air holes on the egg box are sealed with breathable gauze, the egg box is placed in the transport box, one of several heat preservation components is placed above the egg box, and then the box cover is closed and the transport box is sealed for transportation.

10. The transportation method according to claim 9, wherein the bedding material is one or a combination of wood chips, wood shavings, straw, straw powder, puffed corn powder, alfalfa, and timothy grass.

11. The transportation method according to claim 9, wherein the bedding is a mixture of sawdust, rice straw powder and straw, the sawdust and rice straw powder are evenly mixed to serve as the lower bedding, the weight percentage of sawdust in the lower bedding is 20% to 50%, the straw is spread on the mixture of sawdust and rice straw powder to serve as the upper bedding, the upper bedding completely covers the lower bedding, and the black soldier fly eggs and larvae are mixed in the lower bedding.

12. The transportation method according to claim 11, wherein the weight ratio of wood chips to rice straw powder in the lower layer of litter is 1:

1.

13. The transportation method according to claim 11, wherein the weight of the black soldier fly eggs and larvae is 1.5 to 4 times the weight of the lower litter.

14. The transportation method according to claim 9, wherein one or two layers of egg boxes arranged in a rectangular array are placed in the transport box, and the number of egg boxes on the bottom layer is M. Wherein [X] represents rounding, A is the length of the long side of the bottom surface of the transport box, A≤70cm, B is the length of the short side of the bottom surface of the transport box, B≤50cm, d is the thickness of the insulation board, 1cm≤d≤5cm, and the height of the internal space of the transport box is 22-25cm; When there are two layers of egg boxes in the transport box, the number of egg boxes in the upper layer is recorded as N. or And the upper egg boxes are placed alternately with the lower egg boxes.

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