Fermented cordyceps sinensis powder strain culture device and use method therefor

Abstract

The present invention relates to the technical field of production of fermented Cordyceps sinensis powder strains, and provides a fermented Cordyceps sinensis powder strain culture device and a use method therefor. The device comprises an incubator; the incubator comprises a housing; a shaker module, a red / blue light module, an ultraviolet sterilization module, an air pressure adjustment module, a water mist spraying module, a temperature adjustment module, and a negative pressure drainage module are arranged in the housing; a PLC control module is arranged on the outer surface of the housing; the shaker module, the red / blue light module, the ultraviolet sterilization module, the air pressure adjustment module, the water mist spraying module, the temperature adjustment module, and the negative pressure drainage module are electrically connected to the PLC control module. According to the culture device and the use method therefor, the day and night temperature difference is adjusted, the red / blue illumination is properly adjusted, and the humidity condition is controlled, so that the contents of adenosine and ergosterol in the fermented Cordyceps sinensis powder obtained by culture in a triangular shake flask are increased, providing reliable strain sources for later fermentation production in a fermenter

Description

A fermented cordyceps powder culture device and its use method Technical Field

[0001] The invention relates to the technical field of fermented cordyceps sinensis powder strain production, in particular to a fermented cordyceps sinensis powder strain culture device and a use method thereof. Background Art

[0002] Chinese cordyceps, Ophiocordyceps sinensis (Berk.) GHSung et al., is a precious tonic and tonifying Chinese medicinal herb. It exhibits remarkable effects in bidirectional immune regulation, anti-tumor, antioxidant / anti-aging, anti-fatigue, anti-liver damage, anti-diabetes, hypoglycemic and lipid-lowering, antibacterial and anti-inflammatory properties, lung and kidney tonification, hemostasis and phlegm reduction, and promoting vital essence and qi. However, due to the extremely limited availability of natural Cordyceps sinensis, it cannot meet the growing clinical demand. Our fermented Cordyceps powder is produced using a strain isolated from natural Cordyceps by the Institute of Materia Medica, Chinese Academy of Medical Sciences. Toxicological, pharmacological, and chemical studies have shown that its chemical composition and pharmacological effects are essentially identical to those of the original isolate. It can replace natural Cordyceps in the production of Cordyceps products, thus addressing resource shortages. Studies have shown that varying temperature, light, and humidity significantly promote the growth and development of Cordyceps fungi.

[0003] Currently, domestic Cordyceps sinensis culture equipment and methods for use generally utilize open shaking incubators. These devices lack temperature and humidity control systems, requiring external temperature, humidity, and sterilization systems. These devices and methods, still in widespread use, suffer from a lack of versatility and limited specificity. These devices differ significantly from the native environment of the Cordyceps sinensis culture, hindering their full potential and improving the efficiency of subsequent tank fermentation.

[0004] Summary of the Invention

[0005] The present invention aims to provide a fermented Cordyceps sinensis powder culture device and a method for using the same. The culture device and the method for using the present invention can effectively increase the content of adenosine and ergosterol in the fermented Cordyceps sinensis powder obtained by triangular shake flask culture by adjusting the temperature difference between day and night, appropriately adjusting the red-blue light, and controlling the humidity conditions, thereby providing a reliable seed source for subsequent tank fermentation production.

[0006] The technical solutions of the present invention are as follows:

[0007] A fermented Cordyceps sinensis powder spawn cultivation device comprises an incubator, which comprises a shell. A shaking table module, a red-blue light module, an ultraviolet sterilization module, an air pressure regulating module, a water mist spraying module, a temperature regulating module, and a negative pressure drainage module are arranged in the shell. A PLC control module is arranged on the outer surface of the shell. The shaking table module, the red-blue light module, the ultraviolet sterilization module, the air pressure regulating module, the water mist spraying module, the temperature regulating module, and the negative pressure drainage module are electrically connected to the PLC control module.

[0008] As a preferred embodiment of the present invention, the shaker module is placed at the bottom of the shell cavity, the red-blue light module is placed on the rear side of the shell cavity, the ultraviolet sterilization module is placed at the top of the shell cavity, the air pressure regulation module, the water mist spray module, and the temperature regulation module are all placed on the left and right sides of the shell cavity, and the negative pressure drainage module is placed in the lower left part of the shell cavity.

[0009] As a preferred solution of the present invention, the shaking table module is a superimposed shaking table oscillator with an amplitude of 20 to 60 mm and a rotation speed of 100 to 300 rpm, and the process control is completed by a PLC control module.

[0010] As a preferred embodiment of the present invention, the red-blue light module is an LED frequency-adjustable lamp with a power of 6 to 8W, a red light wavelength of 570 to 700nm, and a blue light wavelength of 400 to 500nm. The lighting mode is mainly a day and night mode, that is, red light from 6:00 to 18:00 during the day and blue light from 18:00 to 6:00 at night, and a cosine adjustment cycle mode, that is, the blue light illumination time is increased in the early stage and the red light illumination time is increased in the later stage. The process control is completed by the PLC control module.

[0011] As a preferred solution of the present invention, the ultraviolet sterilization module is an LED frequency-adjustable lamp with a power of 6 to 8W, an ultraviolet wavelength of 265 to 280nm, a sterilization time of 30 to 60 minutes, and process control is completed by a PLC control module.

[0012] As a preferred embodiment of the present invention, the air pressure regulating module is composed of two groups of two-stage Roots pumps connected in series, the air source is connected to the 25±1°C sterile air of the public system, the air pressure is controlled at 56.04±3Kpa during normal operation, and the air pressure is controlled at 101.3±3Kpa during cleaning, the wind speed is 0.5±0.2m / s, and an air pressure sensor and a wind speed sensor are installed in the airway. The process control is completed by the PLC control module.

[0013] As a preferred embodiment of the present invention, the water mist spraying module and the negative pressure drainage module respectively have a water accumulation removal device for adjusting the humidity of the inner cavity of the shell, with a relative humidity of 70-85%. The drainage frequency during normal operation is 60-180 minutes / time, and the drainage frequency during cleaning is continuous. The water source used is purified water from the public system, and the process control is completed by the PLC control module.

[0014] As a preferred solution of the present invention, the temperature regulating module is an electric water loop temperature regulator with a temperature control range of 4 to 60°C and a temperature uniformity of ±0.6°C. The process control is completed by a PLC control module.

[0015] The present invention discloses a method for using any of the above-mentioned culture devices, comprising the following steps:

[0016] 1) Startup and debugging: Turn on the power and start the shaker module, red-blue light module, UV sterilization module, air pressure regulation module, water mist spray module, temperature regulation module, and negative pressure drainage module according to the debugging procedure. Check whether each module is operating normally. After the inspection is completed, the UV sterilization module, air pressure regulation module, and temperature regulation module continue to operate, and UV sterilization lasts for 30 to 60 minutes;

[0017] 2) Shake flask culture: Close the UV sterilization module, adjust the air pressure to normal pressure, open the chamber door, place the triangular shake flask containing the fermented Cordyceps sinensis powder and strain mixture into the shaker module, close the chamber door, and start the shaker module, air pressure control module, water mist spray module, temperature control module, and negative pressure drainage module according to the set program to start the culture;

[0018] 3) Bottle collection: After the incubation time is reached, adjust the air pressure to normal pressure, turn off the shaker module, air pressure adjustment module, water mist spray module, temperature adjustment module, and negative pressure drainage module, open the chamber door, remove the triangular shake flask, and close the chamber door;

[0019] 4) Cleaning: Start the UV sterilization module and air pressure regulation module according to the cleaning procedure to clean and sterilize the inside of the incubator cavity. UV sterilization lasts for 30 to 60 minutes.

[0020] 5) Shutdown: After cleaning and disinfection, turn off the power.

[0021] The beneficial effects of the present invention are as follows: the present invention adjusts the existing strain culture device and the method of use thereof to adapt to the temperature difference between day and night, appropriately adjusts the red-blue light, and controls the humidity conditions, so as to increase the content of adenosine and ergosterol in the fermented Cordyceps sinensis powder obtained by triangular shake flask culture, thereby providing a reliable seed source for subsequent tank fermentation production. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] FIG1 is an external front view of a preferred embodiment of the present invention;

[0023] FIG2 is an internal side view of a preferred embodiment of the present invention;

[0024] FIG3 is an internal top view of a preferred embodiment of the present invention;

[0025] FIG4 is a flow chart of a method of using a preferred embodiment of the present invention;

[0026] In the figure, 1-shell, 2-PLC control module, 3-box door, 4-UV sterilization module, 5-air pressure regulation module, 6-water mist spray module, 7-air temperature regulation module, 8-red-blue light module, 9-negative pressure drainage module, 10-shaking table module. DETAILED DESCRIPTION

[0027] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it should not be understood as a limitation on the scope of protection of the present invention.

[0028] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0029] In the description of the present invention, "several" means one or more, "many" means more than two, "greater than," "less than," and "exceed" are understood to exclude the number itself, while "above," "below," and "within" are understood to include the number itself. The use of "first" and "second" in the description is solely for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, implicitly specifying the number of the indicated technical features, or implicitly specifying the order of the indicated technical features.

[0030] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, and connecting should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0031] 1 to 4, a preferred embodiment of the present invention:

[0032] A fermented Cordyceps sinensis powder spawn culture device includes an incubator, which includes a shell 1. The shell 1 is provided with a shaking table module 10, a red-blue light module 8, an ultraviolet sterilization module 4, an air pressure regulating module 5, a water mist spraying module 6, a temperature regulating module 7, and a negative pressure drainage module 9. A PLC control module 2 is provided on the outer surface of the shell 1. The shaking table module 10, the red-blue light module 8, the ultraviolet sterilization module 4, the air pressure regulating module 5, the water mist spraying module 6, the temperature regulating module 7, and the negative pressure drainage module 9 are electrically connected to the PLC control module 2.

[0033] As a preferred embodiment of the present invention, it may also have the following additional technical features:

[0034] The rocking bed module 10 is arranged at the bottom of the inner cavity of the shell 1, the red-blue light module 8 is arranged at the rear side of the inner cavity of the shell 1, the ultraviolet sterilization module 4 is arranged at the top of the inner cavity of the shell 1, the air pressure regulating module 5, the water mist spraying module 6, and the temperature regulating module 7 are all arranged on the left and right sides of the inner cavity of the shell 1, and the negative pressure drainage module 9 is placed in the lower left part of the inner cavity of the shell 1.

[0035] The shaking table module 10 is a superimposed shaking table oscillator with an amplitude of 20 to 60 mm and a rotation speed of 100 to 300 rpm. The process control is completed by the PLC control module 2.

[0036] The red-blue light module 8 is an LED frequency-adjustable lamp with a power of 6 to 8 W, a red light wavelength of 570 to 700 nm, and a blue light wavelength of 400 to 500 nm. The lighting mode is mainly a day and night mode, that is, red light from 6:00 to 18:00 during the day and blue light from 18:00 to 6:00 at night, and a cosine adjustment cycle mode, that is, the blue light illumination time increases in the early stage and the red light illumination time increases in the later stage. Process control is completed by the PLC control module 2.

[0037] The ultraviolet sterilization module 4 is an LED frequency-adjustable lamp with a power of 6 to 8 W, an ultraviolet wavelength of 265 to 280 nm, a sterilization time of 30 to 60 minutes, and process control is completed by the PLC control module 2.

[0038] The air pressure regulating module 5 is composed of two groups of two-stage Roots pumps connected in series. The air source is connected to the 25±1℃ sterile air of the public system. The air pressure is controlled at 56.04±3KPa during normal operation and at 101.3±3KPa during cleaning. The wind speed is 0.5±0.2m / s. An air pressure sensor and a wind speed sensor are installed in the airway. The process control is completed by the PLC control module 2.

[0039] The water mist spraying module 6 and the negative pressure drainage module 9 respectively provide devices for removing accumulated water for adjusting the humidity of the cavity, which can be drainage pipes or other devices, but are not limited to them. The relative humidity is 70-85%, the drainage frequency during normal operation is 60-180 minutes / time, and the drainage frequency during cleaning is continuous. The water source used is purified water from the public system, and the process control is completed by the PLC control module.

[0040] The temperature regulating module 7 is an electric water loop temperature regulator with a temperature control range of 4 to 60° C. and a temperature uniformity of ±0.6° C. The process control is completed by the PLC control module 2.

[0041] The invention also specifically includes a method for using the above-mentioned culture device, comprising the following steps:

[0042] 1) Startup and debugging: Turn on the power and start the shaking module 10, red-blue light module 8, UV sterilization module 4, air pressure regulation module 5, water mist spray module 6, temperature regulation module 7, and negative pressure drainage module 9 according to the debugging procedure. Check whether each module is operating normally. After the inspection is completed, the UV sterilization module 4, air pressure regulation module 5, and temperature regulation module 7 continue to operate for UV sterilization for 30 to 60 minutes;

[0043] 2) Shake flask culture: Close the UV sterilization module 4, adjust the air pressure to normal pressure, open the chamber door 3, place the triangular shake flask containing the fermented Cordyceps sinensis powder and strain mixture into the shaker module 10, close the chamber door 3, and start the shaker module 10, air pressure regulating module 5, water mist spraying module 6, temperature regulating module 7, and negative pressure drainage module 9 according to the set program to start the culture;

[0044] 3) Bottle collection: After the incubation time is reached, adjust the air pressure to normal pressure, close the shaking table module 10, the air pressure regulating module 5, the water mist spraying module 6, the temperature regulating module 7, and the negative pressure drainage module 9, open the chamber door 3, remove the triangular shaking bottle, and close the chamber door 3;

[0045] 4) Cleaning: Start the UV sterilization module 4 and the air pressure regulating module 5 according to the cleaning program to clean and sterilize the inside of the incubator cavity for 30 to 60 minutes;

[0046] 5) Shutdown: After cleaning and disinfection, turn off the power.

[0047] The present invention is further illustrated by the following specific examples, but is not intended to be limiting.

[0048] Example 1

[0049] 1) Startup and debugging: Turn on the power and start the shaker module according to the debugging program: amplitude 20mm, speed 100rpm; red-blue light module: red light wavelength 570nm, blue light wavelength 400nm, lighting mode day and night mode; UV sterilization module: wavelength 265nm, sterilization time 30min; air pressure regulation module: air pressure 56.04±2Kpa, wind speed 0.5±0.2m / s; water mist spray module: relative humidity 70%; temperature regulation module: temperature 18±2℃; negative pressure drainage module: 120min / time, check whether each module is operating normally. After the inspection is completed, the UV sterilization module, air pressure regulation module, and temperature regulation module continue to operate, and UV sterilization for 30min;

[0050] 2) Shake flask culture: Close the UV sterilization module, adjust the air pressure to normal pressure, open the box door, place the triangular shake flask containing the fermented Cordyceps sinensis powder and strain mixture into the shaker module, close the box door, set the shaker module: amplitude 20 mm, speed 100 rpm; red-blue light module: red light wavelength 570 nm, blue light wavelength 400 nm, light mode day and night mode; UV sterilization module: wavelength 265 nm; air pressure regulation module: air pressure 56.04 ± 0.5 KPa, wind speed 0.5 ± 0.05 m / s; water mist spray module: relative humidity 70%; air temperature regulation module: temperature 15-23 ° C, day and night cosine period change; negative pressure drainage module: 180 min / time, set the culture time to 72 h, start the program to start culture.

[0051] 3) Bottle collection: After the incubation time is reached, adjust the air pressure to normal pressure, turn off the shaker module, red-blue light module, UV sterilization module, air pressure adjustment module, water mist spray module, temperature adjustment module, and negative pressure drainage module, open the box door, remove the triangular shake bottle, and close the box door.

[0052] 4) Cleaning: Set the UV sterilization module to a wavelength of 265 nm and a sterilization time of 30 min; the air pressure regulation module to an air pressure of 101.3 ± 2 kPa and a wind speed of 0.5 ± 0.2 m / s; the water mist spray module to a relative humidity of 85%; and the negative pressure drainage module to continuous. Start the cleaning program to clean and sterilize the interior of the incubator cavity.

[0053] 5) Shutdown: After cleaning, turn off the water mist spray module, UV sterilizer module, negative pressure drainage module, and air pressure regulator module in sequence according to the shutdown procedure. Turn off the power.

[0054] Example 2

[0055] 1) Startup and debugging: Turn on the power and start the shaker module according to the debugging program: amplitude 20mm, speed 100rpm; red-blue light module: red light wavelength 570nm, blue light wavelength 400nm, lighting mode day and night mode; UV sterilization module: wavelength 265nm, sterilization time 30min; air pressure regulation module: air pressure 56.04±2Kpa, wind speed 0.5±0.2m / s; water mist spray module: relative humidity 70%; temperature regulation module: temperature 18±2℃; negative pressure drainage module: 120min / time, check whether each module is operating normally. After the inspection is completed, the UV sterilization module, air pressure regulation module, and temperature regulation module continue to operate, and UV sterilization for 45min;

[0056] 2) Shake flask culture: turn off the UV sterilization module, adjust the air pressure to normal pressure, open the box door, place the triangular shake flask containing the fermented Cordyceps sinensis powder and strain mixture into the shaker module, close the box door, set the shaker module: amplitude 25 mm, speed 200 rpm; red-blue light module: red light wavelength 628 nm, blue light wavelength 450 nm, light mode day and night mode; UV sterilization module: wavelength 270 nm; air pressure regulation module: air pressure 56.04 ± 1 KPa, wind speed 0.5 ± 0.1 m / s; water mist spray module: relative humidity 80%; air temperature regulation module: temperature 10-23 ° C, day and night cosine cycle change; negative pressure drainage module: 120 min / time, set the culture time to 72 h, start the program to start culture.

[0057] 3) Bottle collection: After the incubation time is reached, adjust the air pressure to normal pressure, turn off the shaker module, red-blue light module, UV sterilization module, air pressure adjustment module, water mist spray module, temperature adjustment module, and negative pressure drainage module, open the box door, remove the triangular shake bottle, and close the box door.

[0058] 4) Cleaning: Set the UV sterilization module to a wavelength of 265 nm and a sterilization time of 45 min; the air pressure regulation module to an air pressure of 101.3 ± 2 kPa and a wind speed of 0.5 ± 0.2 m / s; the water mist spray module to a relative humidity of 85%; and the negative pressure drainage module to continuous. Start the cleaning program to clean and sterilize the interior of the incubator cavity.

[0059] 5) Shutdown: After cleaning, turn off the water mist spray module, UV sterilizer module, negative pressure drainage module, and air pressure regulator module in sequence according to the shutdown procedure. Turn off the power.

[0060] Example 3

[0061] 1) Startup and debugging: Turn on the power and start the shaker module according to the debugging program: amplitude 20mm, speed 100rpm; red-blue light module: red light wavelength is 700nm, blue light wavelength is 500nm, and the illumination mode is cycle mode; UV sterilization module: wavelength is 265nm, sterilization time is 30min; air pressure regulation module: air pressure 56.04±2Kpa, wind speed 0.5±0.2m / s; water mist spray module: relative humidity 70%; temperature regulation module: temperature 18±2℃; negative pressure drainage module: 120min / time, check whether each module is operating normally. After the inspection is completed, the UV sterilization module, air pressure regulation module, and temperature regulation module continue to operate, and UV sterilization is 60min;

[0062] 2) Shake flask culture: Close the UV sterilization module, adjust the air pressure to normal pressure, open the box door, place the triangular shake flask containing the fermented Cordyceps sinensis powder and strain mixture into the shaker module, close the box door, and set the shaker module: amplitude 30 mm, speed 300 rpm; red-blue light module: red light wavelength 570 nm, blue light wavelength 400 nm, illumination mode cosine regulation cycle mode; UV sterilization module: wavelength 280 nm; air pressure regulation module: air pressure 56.04 ± 2 KPa, wind speed 0.5 ± 0.2 m / s; water mist spray module: relative humidity 85%; air temperature regulation module: temperature 5-23 ° C, cosine cycle change during the day and night; negative pressure drainage module: 60 min / time, set the culture time to 72 h, and start the program to start culture.

[0063] 3) Bottle collection: After the incubation time is reached, adjust the air pressure to normal pressure, turn off the shaker module, red-blue light module, UV sterilization module, air pressure adjustment module, water mist spray module, temperature adjustment module, and negative pressure drainage module, open the box door, remove the triangular shake bottle, and close the box door.

[0064] 4) Cleaning: Set the UV sterilization module to a wavelength of 265 nm and a sterilization time of 60 min; the air pressure regulation module to an air pressure of 101.3 ± 2 kPa and a wind speed of 0.5 ± 0.2 m / s; the water mist spray module to a relative humidity of 85%; and the negative pressure drainage module to continuous. Start the cleaning program to clean and sterilize the interior of the incubator cavity.

[0065] 5) Shutdown: After cleaning, turn off the water mist spray module, UV sterilizer module, negative pressure drainage module, and air pressure regulator module in sequence according to the shutdown procedure. Turn off the power.

[0066] The fermented Cordyceps sinensis powder of the above examples was subjected to quality testing, and the results are shown in Table 1.

[0067] Table 1 Quality results of fermented Cordyceps sinensis powder obtained in Examples 1-3

[0068] Example 4 Comparative Experiment

[0069] In order to investigate the optimal culture device and method for fermenting Cordyceps sinensis powder, a comparative experiment was conducted between the culture device and method of the present invention and the existing culture device and method of use.

[0070] Plan 1: Constant temperature and humidity incubator A: temperature: 18 ± 1°C; relative humidity: 70 ± 5%; shaker: amplitude 20 mm, speed 200 rpm; incubation time 72 h;

[0071] Solution 2: Constant temperature and humidity incubator B: temperature: 20 ± 1°C; relative humidity: 80 ± 5%; shaker: amplitude 25 mm, speed 300 rpm; incubation time 72 h;

[0072] Plan 3: Biochemical incubator: temperature: 16 ± 5°C; relative humidity: 85 ± 5%; shaker: amplitude 25 mm, speed 300 rpm; incubation time 72 h;

[0073] Solution 4: The culture device of the present invention and its use method (Example 2);

[0074] Solution 5: The culture device of the present invention and its use method (Example 3).

[0075] The fermented Cordyceps sinensis powder of the comparative experiment of the embodiment was subjected to quality inspection, and the results are shown in Table 2.

[0076] Table 2 The quality of the fermented Cordyceps powder obtained in Example 4

[0077] As can be seen from Tables 1 and 2, the adenosine, ergosterol, and total glycosides in the bacterial powder cultured by the culture device of the present invention (Schemes 4 and 5) are all higher than those in Schemes 1-3, and the adenosine content of Examples 1-3 is all above 0.35%, the ergosterol content is all above 0.56%, and the total glycosides content is all above 1.2%. This shows that the bacterial culture device used in the present invention and the method of use thereof increase the content of adenosine and ergosterol in the fermented Cordyceps sinensis powder obtained by triangular shake flask culture by adjusting the day and night temperature difference, appropriately adjusting the red-blue light, and controlling the humidity conditions, thereby providing a reliable seed source for subsequent tank fermentation production.

[0078] Under the premise that no conflict occurs, those skilled in the art may freely combine and superimpose the above-mentioned additional technical features.

[0079] The above-described embodiments merely represent preferred implementations of the present invention. While the descriptions thereof are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make various other corresponding changes and modifications based on the technical solutions and concepts described above, and all such changes and modifications should fall within the scope of protection of the claims of the present invention.

Claims

1. A fermented Cordyceps sinensis powder culture device, comprising a culture box, characterized in that: The incubator comprises a shell (1), wherein a shaking table module (10), a red-blue light module (8), an ultraviolet sterilization module (4), an air pressure regulating module (5), a water mist spraying module (6), an air temperature regulating module (7), and a negative pressure drainage module (9) are arranged inside the shell (1), and a PLC control module (2) is arranged on the outer surface of the shell (1), and the shaking table module (10), the red-blue light module (8), the ultraviolet sterilization module (4), the air pressure regulating module (5), the water mist spraying module (6), the air temperature regulating module (7), and the negative pressure drainage module (9) are electrically connected to the PLC control module (2).

2. A fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The shaking bed module (10) is arranged at the bottom of the inner cavity of the shell (1), the red-blue light module (8) is arranged at the rear side of the inner cavity of the shell (1), the ultraviolet sterilization module (4) is arranged at the top of the inner cavity of the shell (1), the air pressure regulating module (5), the water mist spraying module (6), and the air temperature regulating module (7) are all arranged on the left and right sides of the inner cavity of the shell (1), and the negative pressure drainage module (9) is placed at the lower left part of the inner cavity of the shell (1).

3. A fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The shaking table module (10) is a superimposed shaking table oscillator with an amplitude of 20 to 60 mm and a rotation speed of 100 to 300 rpm. The process control is completed by a PLC control module (2).

4. The fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The red-blue light module (8) is an LED frequency-adjustable lamp with a power of 6-8W, a red light wavelength of 570-700nm, and a blue light wavelength of 400-500nm. The specific lighting mode is: red light from 6:00 to 18:00 during the day, and blue light from 18:00 to 6:00 at night, combined with a cosine regulation cycle mode. The process control is completed by the PLC control module (2).

5. The fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The ultraviolet sterilization module (4) is an LED frequency-adjustable lamp with a power of 6 to 8 W, an ultraviolet wavelength of 265 to 280 nm, a sterilization time of 30 to 60 minutes, and process control is completed by a PLC control module (2).

6. The fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The air pressure regulating module (5) is composed of two groups of two-stage Roots pumps connected in series, and the air source is connected to the 25±1°C sterile air from the public system. The air pressure is controlled at 56.04±3Kpa during normal operation and at 101.3±3Kpa during cleaning. The wind speed is 0.5±0.2m / s. An air pressure sensor and a wind speed sensor are installed in the airway, and the process control is completed by the PLC control module (2).

7. The fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The water mist spraying module (6) and the negative pressure drainage module (9) are respectively provided with a water accumulation removal device for adjusting the humidity of the inner cavity of the shell, with a relative humidity of 70-85%, a drainage frequency of 60-180 minutes / time during normal operation, and a drainage frequency of continuous during cleaning, and process control is completed by a PLC control module.

8. The fermented Cordyceps sinensis powder culture device according to claim 1, characterized in that: The temperature regulating module (7) is an electric hot water loop temperature regulator with a temperature control range of 4 to 60° C. and a temperature uniformity of ±0.6° C. The process control is completed by the PLC control module (2).

9. A method for using the culture device according to any one of claims 1 to 8, characterized in that: The following steps are involved: Start-up debugging: Turn on the power supply, start the shaking table module (10), the red-blue light module (8), the ultraviolet sterilization module (4), the air pressure regulating module (5), the water mist spraying module (6), the air temperature regulating module (7), and the negative pressure drainage module (9) according to the debugging procedure, and check whether each module is operating normally. After the inspection is completed, the ultraviolet sterilization module (4), the air pressure regulating module (5), and the air temperature regulating module (7) continue to operate, and the ultraviolet sterilization is carried out for 30 to 60 minutes; Shake flask culture: close the ultraviolet sterilization module (4), adjust the air pressure to normal pressure, open the box door (3), put the triangular shake flask containing the fermented Cordyceps sinensis powder and strain mixture into the shaker module (10), close the box door (3), start the shaker module (10), the air pressure regulating module (5), the water mist spraying module (6), the air temperature regulating module (7), and the negative pressure drainage module (9) according to the set program, and start the culture; Bottle collection: After the incubation time is reached, the air pressure is adjusted to normal pressure, the shaking table module (10), the air pressure regulating module (5), the water mist spraying module (6), the air temperature regulating module (7), and the negative pressure drainage module (9) are closed, the box door (3) is opened, the triangular shaking bottle is taken out, and the box door (3) is closed; Cleaning: Start the UV sterilization module (4) and the air pressure regulating module (5) according to the cleaning procedure. Clean and sterilize the inside of the incubator cavity, and sterilize with ultraviolet light for 30 to 60 minutes; Shutdown: After cleaning and disinfection, turn off the power.

Images