Targeted proteomics for monitoring autophagy
The method employs mass spectrometry with a peptide mix targeting SAR proteins to monitor autophagy selectivity and protein regulation, addressing the limitations of existing techniques by enabling high-throughput detection of autophagy activity and subtype complexity.
Patent Information
- Application Number
- PCT/EP2024/068339
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-03-04
- Filing Date
- 2024-06-28
- Publication Date
- 2025-01-02
AI Technical Summary
Current methods for studying autophagy are limited by their inability to detect changes in protein abundance in a high-throughput manner, particularly for primary cells and large-scale screening approaches, and often require significant protein amounts or protein tagging, which is unsuitable for all sample types and fails to capture the complexity of stress-induced autophagy.
A method using mass spectrometry to monitor autophagy selectivity by probing samples for the amount of different selective autophagy receptor (SAR) proteins with a peptide mix comprising peptides derived from multiple SAR proteins, allowing for the detection of various autophagy subtypes and protein regulation changes.
Enables high-throughput detection of autophagy selectivity and protein regulation changes, providing a robust and sensitive method for monitoring autophagy activity across different conditions, with improved sensitivity and accuracy compared to traditional methods.
Smart Images
Figure IMGF000007_0001 
Figure IMGF000008_0001 
Figure IMGF000009_0001
Abstract
Citation Information
Patent Citations
Organ-specific proteins and methods of their use
WO2008021290A2
DETECTION AND QUANTIFICATION OF AKT-mTOR PATHWAY PROTEINS
WO2017160698A1
Proteogenomic analysis of non-small cell lung cancer
WO2022207671A1