Rat MCAO modeling animal fixing apparatus

Abstract

A rat MCAO modeling animal fixing apparatus, comprising a bottom plate, and a head fixing member, a front claw fixing member, and rear claw fixing members arranged on a top surface of the bottom plate. The front claws are fixed by using the front claw fixing member, and two middle screws each having a front claw hook-and-loop fastener are arranged on a transverse rail. According to the straightened distance of the front claws, the middle screws are tightened to be fixed on the transverse rail, and then fixing of the upper part of the animal is complete. Rear claw hook-and-loop fasteners on each rear claw fixing member may be fixed at any position on a circular surface having a radius of 5 cm centered on a bottom screw. When the head is fixed, one end of an elastic rubber band is hung on a top fixing rod to control the tightness of the elastic rubber band, and the height may be adjusted according to the thickness of the animal head. The other end of the elastic rubber band is pulled straight and hung on the incisors of the upper jaw of the rat to fix the head. The apparatus solves the problem in traditional methods of an animal not being firmly fixed, which affects the experimental effect and efficiency, and facilitates pulling out the tongue of the animal to prevent the animal from suffocating during the experiment.

Description

A rat MCAO model animal fixation device Technical Field

[0001] The invention relates to the technical field of animal modeling, in particular to an animal fixing device for MCAO modeling of rats. Background Art

[0002] According to the World Health Organization's "2020 Global Health Estimates," seven of the top 10 causes of death in 2019 were noncommunicable diseases. Stroke was the second leading cause of death, accounting for 11% of all deaths. Stroke, also known as "cerebral stroke," has become one of the most pressing global public health issues in recent years. Stroke includes both ischemic and hemorrhagic strokes. Ischemic stroke has a higher incidence than hemorrhagic stroke, accounting for 60% to 70% of all strokes. Stroke is associated with high morbidity, disability, recurrence, and mortality. Due to the lack of effective treatments, animal models have become crucial tools for understanding the pathogenesis of the disease and researching effective therapeutic drugs. Currently, the internationally recognized middle cerebral artery occlusion (MCAO) model is commonly used as a model of cerebral ischemia. Its pathogenesis is similar to that of human ischemic stroke, making it crucial for developing models that simulate human cerebral ischemia, understanding its pathogenesis, and for drug screening.

[0003] Rat stroke models are particularly commonly used. Currently, the main methods for establishing animal models include craniotomy and mechanical occlusion, photochemical methods, suture embolization, and embolization. Suture embolization has become the most commonly used and classic modeling method in recent years. The neck vessels are isolated and exposed, and a nylon suture is inserted through the bifurcation of the external carotid artery (ECA) or common carotid artery (CCA) into the internal carotid artery (ICA). This suture occludes the origin of the middle cerebral artery (MCA) and all its collaterals, resulting in focal ischemia in the MCA region. During these animal modeling procedures, all surgical procedures are typically performed on a single platform, and the anesthetized animal must be securely fixed, otherwise experimental efficiency and modeling effectiveness will be compromised. Technical issues

[0004] The present invention proposes a rat MCAO modeling animal fixation device, which solves the problem that traditional fixation methods such as tape fixation are not firm and easy to move, affecting the experimental effect and efficiency. It also facilitates pulling out the animal's tongue to prevent the anesthetized animal from suffocating during the experiment. Technical Solutions

[0005] The technical solution of the present invention is as follows: a rat MCAO model animal fixation device, comprising a bottom plate, a head fixation member, a front paw fixation member, and a hind paw fixation member provided on the top surface of the bottom plate, wherein the three fixation members are vertically spaced 10 cm apart;

[0006] The head fixing part includes a top fixing rod and an elastic rubber band. A top threaded groove is opened on the bottom plate at a position corresponding to the head fixing part. A threaded rod is fixedly installed on the bottom of the top fixing rod. The threads on the threaded rod match the threads in the top threaded groove. The top fixing rod is fixedly installed on the bottom plate through the threaded rod, and the elastic rubber band is movably installed on the top fixing rod.

[0007] The front paw fixing part includes a front paw Velcro, a middle screw, and a slider. A movable groove is opened on the bottom plate at a position corresponding to the front paw fixing part. The slider is installed in the movable groove. A middle thread groove is opened on the slider. The thread on the middle screw matches the texture in the middle thread groove. The middle screw is movably installed on the slider. The front paw Velcro is installed on the top of the middle screw.

[0008] The rear grab fixing part includes a cross bar, a bottom screw, and a rear grab Velcro. The length of the bottom screw is 5 cm. A bottom thread groove is provided on the bottom plate at a position corresponding to the rear claw fixing part. The thread on the bottom screw matches the texture in the bottom thread groove. The bottom screw is fixedly installed on the bottom plate through the bottom plate. A connecting groove is provided in the middle of the cross bar. The cross bar can be movably mounted on the bottom screw through the connecting groove. The rear grab Velcro is fixedly installed on the top of the cross bar. The rear grab Velcro is equivalent to being fixed at any position on a circular surface with a radius of 5 cm with the bottom screw as the center.

[0009] Preferably, spacers are fixedly installed at equal intervals on the outer side of the top fixing rod, and the elastic rubber band can be stuck between the spacers.

[0010] Preferably, a rotating block is movably mounted on the top of the middle screw, and the front paw Velcro is fixedly mounted on the rotating block.

[0011] Preferably, a sliding groove is provided on the inner wall of the movable groove, and both ends of the slider are movably clamped in the sliding groove, and the slider is movably installed in the movable groove through the sliding groove.

[0012] Preferably, a circle of wavy lines is provided on the top surface of the crossbar.

[0013] Preferably, a pick is fixedly mounted on the top of the bottom screw.

[0014] Preferably, the height of the top fixing rod is 5 cm.

[0015] Preferably, the elastic rubber band is preferably an ordinary rubber band. Beneficial effects

[0016] The working principle and beneficial effects of the present invention are:

[0017] 1. In the present invention, the head fixing part, the front paw fixing part, and the hind paw fixing part cooperate with each other to quickly fix the rat on the base plate. At the same time, the position of the rat can be easily adjusted after fixation, avoiding the inconvenience of adjusting the position of the rat when fixing the rat with tape.

[0018] 2. In the present invention, the top fixing rod can be quickly fixed to the bottom plate through the threaded rod, and the tightness of the elastic rubber band can be controlled by adjusting the height of one end of the elastic rubber band hanging on the top fixing rod according to the size of the animal. The spacer on the top fixing rod can prevent the elastic rubber band from sliding when fixed on the top fixing rod. The other end of the elastic rubber band is straightened and hung on the incisor teeth of the upper jaw of the rat to fix the head, making it easy to pull out the tongue, reducing the risk of suffocation, and avoiding the problem of suffocation of rats when fixing the head of anesthetized rats in the traditional fixing method.

[0019] 3. The present invention can effectively solve the problem of animal fixation during the modeling process, ensure that the animals remain motionless during the experiment, and is suitable for experimental rats of different body lengths, making it convenient for scientific researchers to perform surgical operations, thereby improving the efficiency and modeling effect of animal modeling experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0021] FIG1 is a schematic diagram of the overall structure of the present invention.

[0022] FIG2 is a schematic diagram of point A in FIG1 of the present invention.

[0023] FIG3 is a schematic diagram of point B in FIG1 of the present invention.

[0024] FIG4 is a schematic diagram of a partial structure of the present invention.

[0025] In the picture:

[0026] 1. Bottom plate; 2. Top threaded groove; 3. Top fixing rod; 4. Elastic band; 5. Movable groove; 6. Crossbar; 7. Bottom screw; 8. Rear grip Velcro; 9. Threaded rod; 10. Wavy pattern; 11. Bottom threaded groove; 12. Connecting groove; 13. Pick; 14. Front claw Velcro; 15. Middle screw; 16. Slide groove; 17. Slider; 18. Rotating block; 19. Spacer; 20. Middle threaded groove. Modes for Carrying Out the Invention

[0027] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0028] Referring to Figures 1-4, the present invention provides a technical solution for a fixation device for MCAO modeling of rats: comprising a base plate 1, on the top surface of which are provided a head fixation member, a front paw fixation member, and a hind paw fixation member, with the three fixation members vertically spaced 10 cm apart.

[0029] The head fixing part includes a top fixing rod 3 and an elastic rubber band 4. A top threaded groove 2 is opened on the bottom plate 1 at a position corresponding to the head fixing part. A threaded rod 9 is fixedly installed at the bottom of the top fixing rod 3. The threads on the threaded rod 9 match the threads in the top threaded groove 2. The top fixing rod 3 is fixedly installed on the bottom plate 1 through the threaded rod 9. The elastic rubber band 4 is movably installed on the top fixing rod 3. The top fixing rod 3 can be quickly fixed to the bottom plate 1 through the threaded rod 9. One end of the elastic rubber band 4 is put under the top of the top fixing rod 3, and the other end is straightened and hung on the two incisors of the rat's upper jaw threaded groove to fix the animal's head. It is also convenient to pull out the tongue, reduce the risk of suffocation, and avoid the problem that the rat may suffocate when fixing the head of anesthetized rats with traditional fixation methods.

[0030] The top threaded rod 9 is fixed, and the elastic rubber band 4 is put on the threaded rod 9. The height can be adjusted appropriately according to the thickness of the animal's head. The elastic rubber band 4 is similar to an ordinary rubber band and can be taken down. One end of the elastic rubber band 4 is hung on the threaded rod 9, and the other end is used to hang the two incisors of the animal's upper jaw. At this time, the animal's head is tilted to one side with the ventral side facing up. Use tweezers to pull out the animal's tongue to prevent the animal from suffocating.

[0031] The front paw fixing part includes a front paw Velcro 14, a middle screw 15, and a slider 17. A movable groove 5 is opened on the bottom plate 1 at the position corresponding to the front paw fixing part. The slider 17 is installed in the movable groove 5. A middle thread groove 20 is opened on the slider 17. The thread on the middle screw 15 matches the texture in the middle thread groove 20. The middle screw 15 is movably installed on the slider 17. The front paw Velcro 14 is installed on the top of the middle screw 15. Rotating the middle screw 15 can move the middle screw 15 downward. By cooperating with the slider 17, the bottom plate 1 can be clamped, thereby fixing the front paw Velcro 14 in the current position, which is convenient for fixing the rat's front paw;

[0032] The front paws are fixed with the front paw fixing parts. There are two movable bolts with front paw Velcro 14 on the movable groove 5 cross rail. According to the distance after the front paws are straightened, tighten the middle screw 15 and fix it on the cross rail. The animal's front paws are horizontally in a line, and the part of the animal above the cross bar is fixed.

[0033] The rear grab fixing part includes a cross bar 6, a bottom screw 7, and a rear grab Velcro 8. A bottom thread groove 11 is provided on the base plate 1 at the position corresponding to the rear claw fixing part. The thread on the bottom screw 7 matches the texture in the bottom thread groove 11. The bottom screw 7 is fixedly installed on the base plate 1 through the base plate 1. A connecting groove 12 is provided in the middle of the cross bar 6. The cross bar 6 can be movably sleeved on the bottom screw 7 through the connecting groove 12. The rear grab Velcro 8 is fixedly installed on the top of the cross bar 6. The position of the rear grab Velcro 8 is movable. After adjusting the position of the rear grab Velcro 8, it is fixed; the bottom screw 7 is fixed to the base plate 1 through the bottom thread groove 11, and the cross bar 6 is sleeved under the top of the bottom screw 7 through the connecting groove 12. According to the size of the animal, the cross bar 6 can be rotated to adjust the rear grab Velcro 8 to fix the animal's hind claw part, and then the bottom screw 7 is tightened to fix the position of the cross bar 6.

[0034] In order to increase the range of activity, rats of different sizes are used. The hind paw fixer is a fixing piece with a rear-grip Velcro 8, which can be slid freely on the crossbar 6 (5 cm), slid to the appropriate position, and tightened to fix it. The bottom screw 7 can be tightened to fix the crossbar 6 at any time. The rear-grip Velcro 8 can be fixed at any position on a circular surface with a radius of 5 cm centered on the bottom screw 7. In this way, the hind paw fixator can fix animals of any body length and width.

[0035] Furthermore, spacers 19 are evenly and equidistantly fixed on the outer side of the top fixing rod 3, and the elastic rubber band 4 can be stuck between the spacers 19. The spacers 19 can prevent the elastic rubber band 4 from sliding when fixed on the top fixing rod 3, causing the head fixation to loosen.

[0036] Furthermore, a rotating block 18 is movably installed on the top of the middle screw 15, and the front paw Velcro 14 is fixedly installed on the rotating block 18. When the middle screw 15 is rotated, the rotating block 18 can prevent the front paw Velcro 14 from rotating with it, thereby facilitating the adjustment of the angle of the front paw Velcro 14.

[0037] Furthermore, a sliding groove 16 is opened on the inner wall of the movable groove 5, and the two ends of the slider 17 are movably clamped in the sliding groove 16. The slider 17 is movably installed in the movable groove 5 through the sliding groove 16. The sliding groove 16 can enable the slider 17 to drive the middle screw 15 to move in the movable groove 5, thereby facilitating the adjustment of the position of the front paw Velcro 14 on the base plate 1.

[0038] Furthermore, a circle of wave patterns 10 is provided on the top surface of the cross bar 6. When the bottom screw 7 fixes the cross bar 6, the wave patterns 10 can increase the friction between the bottom screw 7 and the cross bar 6, so that the fixation can be more stable.

[0039] Furthermore, a paddle 13 is fixedly mounted on the top of the bottom screw 7, and the paddle 13 can be used to conveniently manually rotate the bottom screw 7 for easy adjustment.

[0040] Furthermore, the height of the top fixing rod 3 is 5 cm, and the height of the elastic rubber band 4 hanging on the top fixing rod 3 can be adjusted according to the size of the animal, and the tightness of the elastic rubber band 4 can be controlled to facilitate fixing the rat's head.

[0041] Furthermore, the elastic rubber band 4 is preferably an ordinary rubber band, which is made of simple materials and is easy to obtain.

[0042] Working principle of the present invention:

[0043] When using this device, after selecting the rat for experiment, anesthetize it, spread the rat on the base plate 1, move the front paw Velcro 14 to the position of the rat's front paw and fix it, then rotate the middle screw 15 to cooperate with the slider 17 to clamp the base plate 1, thereby fixing the front paw Velcro 14 in the current position, and then put the cross bar 6 on the bottom screw 7, and the bottom screw 7 can be fixed to the base plate 1 through the bottom thread groove 11. According to the size of the animal, the cross bar 6 can be rotated to adjust the rear grip Velcro 8 to fix the animal's hind paw part, and then tighten the bottom screw 7 to fix the position of the cross bar 6. Finally, fix the top fixing rod 3 to the base plate 1 through the threaded rod 9, put one end of the elastic rubber band 4 under the top of the top fixing rod 3, and straighten the other end to hang on the two incisors of the rat's upper jaw top thread groove to fix the animal's head and facilitate pulling out the tongue to reduce the risk of suffocation. At this time, the experiment can be carried out.

[0044] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A rat MCAO modeling animal fixation device, comprising a bottom plate (1), characterized in that: The top surface of the bottom plate (1) is provided with a head fixing part, a front paw fixing part, and a rear paw fixing part, and the vertical interval between the three fixing parts is 10 cm; The head fixing part comprises a top fixing rod (3) and an elastic rubber band (4); a top thread groove (2) is provided on the bottom plate (1) at a position corresponding to the head fixing part; a thread rod (9) is fixedly installed at the bottom of the top fixing rod (3); the thread on the thread rod (9) matches the thread in the top thread groove (2); the top fixing rod (3) is fixedly installed on the bottom plate (1) via the thread rod (9); and the elastic rubber band (4) is movably installed on the top fixing rod (3); The front paw fixing member comprises a front paw Velcro (14), a middle screw (15), and a slider (17); a movable groove (5) is provided on the bottom plate (1) at a position corresponding to the front paw fixing member; the slider (17) is installed in the movable groove (5); a middle thread groove (20) is provided on the slider (17); the thread on the middle screw (15) matches the texture in the middle thread groove (20); the middle screw (15) is movably installed on the slider (17); and the front paw Velcro (14) is installed on the top of the middle screw (15); The rear grip fixing member comprises a cross bar (6), a bottom screw (7), and a rear grip Velcro (8); a bottom thread groove (11) is provided on the bottom plate (1) at a position corresponding to the rear claw fixing member; the thread on the bottom screw (7) matches the pattern in the bottom thread groove (11); the bottom screw (7) is fixedly mounted on the bottom plate (1) through the bottom plate (1); a connecting groove (12) is provided in the middle of the cross bar (6); the cross bar (6) can be movably sleeved on the bottom screw (7) through the connecting groove (12); and the rear grip Velcro (8) is mounted on the top of the cross bar (6).

2. A rat MCAO modeling animal fixation device according to claim 1, characterized in that: Spacers (19) are evenly and equidistantly fixedly mounted on the outer side of the top fixing rod (3), and the elastic rubber band (4) can be stuck between the spacers (19).

3. A rat MCAO modeling animal fixation device according to claim 1, characterized in that: A rotating block (18) is movably mounted on the top of the middle screw (15), and the front paw Velcro (14) is fixedly mounted on the rotating block (18).

4. A rat MCAO modeling animal fixation device according to claim 1, characterized in that: A slide groove (16) is provided on the inner wall of the movable groove (5), and both ends of the slider (17) are movably clamped in the slide groove (16). The slider (17) is movably installed in the movable groove (5) through the slide groove (16).

5. The rat MCAO modeling animal fixing device according to claim 1, characterized in that: A circle of wavy patterns (10) is provided on the top surface of the crossbar (6).

6. A rat MCAO modeling animal fixation device according to claim 1, characterized in that: A paddle (13) is fixedly mounted on the top of the bottom screw (7).

7. The rat MCAO modeling animal fixation device according to claim 1, characterized in that: The height of the top fixing rod (3) is 5 cm.

8. The rat MCAO modeling animal fixing device according to claim 1, characterized in that: The elastic rubber band (4) is preferably an ordinary rubber band.

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