Environmentally-friendly biological liquid fertilizer and preparation method therefor

Abstract

Provided are an environmentally-friendly biological liquid fertilizer and a preparation method therefor. The environmentally-friendly biological liquid fertilizer comprises the following components in parts by weight: 95-105 parts of a microcapsule microbial inoculant liquid fertilizer, 10-15 parts of an amino acid fertilizer, 8-12 parts of glucose, 7-10 parts of potassium humate, and 400-500 parts of water, wherein the microcapsule microbial inoculant liquid fertilizer is a suspension formed by a microbial inoculant microcapsule in an inorganic fertilizer solution, and the microbial inoculant microcapsule is a hydrophobic microcapsule prepared by coating a microbial inoculant with sodium lignin sulfonate-corn starch. By means of preparing the hydrophobic microcapsule, the technical effect of coating a microbial inoculant to avoid an environmental impact is achieved, a variety of microbial inoculants are separately encapsulated and are dispersed in the inorganic fertilizer solution, and an organic fertilizer and a carbon source are supplemented to prepare the composite biological liquid fertilizer, thereby achieving the technical effects of easy absorption of the fertilizer, prevention against microbial antagonism, an increased survival rate and water-fertilizer integration.

Description

Environmentally friendly biological liquid fertilizer and preparation method Technical Field

[0001] The present invention belongs to the field of fertilizers, and in particular relates to an environmentally friendly biological liquid fertilizer and a preparation method thereof. Background Art

[0002] Liquid fertilizer is a high-concentration fluid fertilizer containing one or more nutrients necessary for the growth of crops. It is easier for plants to absorb than traditional solid fertilizers and is mainly divided into two categories. One is liquid nitrogen fertilizer, whose main nutrient component is a single nitrogen element; the other is liquid compound fertilizer, which contains a variety of common nutrients, as well as nutrients and trace elements added according to actual needs; biological fertilizer is fertilizer obtained by fermentation or cultivation of biomass materials or microbial strains, which can provide the nutrients needed by plants and improve soil structure and ecological environment, reduce pollution to the environment, and achieve sustainable agricultural development; environmentally friendly fertilizer refers to fertilizer that is friendly to the environment and reduces negative impacts on ecosystems and human health. The use of environmentally friendly fertilizers can reduce the negative impact of chemical synthetic fertilizers on the environment, protect the health of soil and water bodies, and at the same time improve the yield and quality of crops.

[0003] The current existing technologies mainly have the following problems: 1. Fertilizers are easily lost during application, the amount absorbed by plants is small and the utilization rate is low, and large-scale and repeated fertilization wastes manpower and material resources and has adverse effects on the environment; 2. The complex microbial flora in liquid compound microbial fertilizers are easily antagonistic, have low survival rates, are difficult to preserve, and have a short shelf life.

[0004] Summary of the Invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides an environmentally friendly biological liquid fertilizer and a preparation method thereof. In order to solve the problems of easy loss of fertilizer nutrients and short shelf life of liquid composite microbial fertilizer, the present invention proposes to prepare hydrophobic microcapsules to achieve the technical effect of encapsulating microbial agents to avoid environmental impact, encapsulate multiple bacterial agents separately, disperse them in inorganic fertilizer solution, and supplement them with organic fertilizer and carbon source to prepare composite biological liquid fertilizer, thereby achieving the technical effects of easy fertilizer absorption, avoiding microbial antagonism, improving survival rate and water-fertilizer integration.

[0006] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is as follows: The present invention proposes an environmentally friendly biological liquid fertilizer and a preparation method thereof, wherein the environmentally friendly biological liquid fertilizer comprises the following components in parts by weight: 95-105 parts of microcapsule bacterial agent liquid fertilizer, 10-15 parts of amino acid fertilizer, 8-12 parts of glucose, 7-10 parts of potassium humate, and 400-500 parts of water.

[0007] Preferably, the microcapsule bacterial agent liquid fertilizer is a suspension formed by bacterial agent microcapsules in an inorganic fertilizer solution;

[0008] Preferably, the bacterial agent microcapsules are hydrophobic microcapsules prepared by encapsulating the bacterial agent with sodium lignin sulfonate-corn starch.

[0009] Preferably, the microcapsule bacterial agent liquid fertilizer comprises the following components in parts by weight: 0.25-0.35 parts of bacterial powder, 50-60 parts of corn starch, 6-8 parts of sodium lignin sulfonate, 16-19 parts of glycerol, 0.8-1.2 parts of stearic acid, 100-150 parts of dipotassium hydrogen phosphate, 200-250 parts of urea, and 1000-1200 parts of water.

[0010] Preferably, the method for preparing the microcapsule bacterial agent liquid fertilizer specifically comprises the following steps:

[0011] S1. Take bacterial powder and add it to sterile water, add Tween-80 dropwise, and cool it to 30-40°C in a 60-70°C water bath for 15-25 minutes. Place it on a shaker and shake at 200-300 rpm for 30-40 minutes to obtain a bacterial suspension.

[0012] S2. The bacterial suspension obtained in S1 was inoculated into a liquid culture medium with a pH of 7 and a mass ratio of peptone: beef extract powder: NaCl: agar: distilled water of 5:3:5:15:1000, and the culture medium was placed on a shaker in a water bath for 10-12 hours to obtain a fermentation seed liquid;

[0013] S3: The fermentation seed liquid obtained in S2 was inoculated into a new liquid culture medium at a concentration of 1% by volume, and cultured on a shaker at a speed of 150-180 rpm for 30-40 h. The culture was centrifuged and resuspended in liquid culture medium to obtain an effective viable cell count of ≥ 4 × 10 10 CFU / mL of bacterial solution;

[0014] S4. Mix corn starch and water in a mass ratio of 1:9-12, add sodium lignin sulfonate and glycerol in sequence, stir evenly to obtain a wall material solution, add the bacterial solution obtained in S3, and stir in a water bath at 50°C at a speed of 300 rpm for 20 minutes to mix evenly, then add stearic acid, continue stirring in a water bath for 1 hour, cool to room temperature, and spray dry to obtain a microcapsule bacterial agent;

[0015] S5, adding dipotassium hydrogen phosphate and urea to water, heating and stirring until completely dissolved, to obtain an inorganic fertilizer solution;

[0016] S6. Evenly mix the mixed microcapsule bacterial agent obtained in S4 and the inorganic fertilizer solution obtained in S5 to obtain an effective viable bacterial count of ≥4×10 9 CFU / mL of microencapsulated bacterial liquid fertilizer.

[0017] Preferably, in S1, the bacterial powder is one or more of Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus megaterium, Bacillus amyloliquefaciens, and Bacillus mucilaginosus;

[0018] Preferably, in S1, the amount of Tween-80 added to water is 2.2-3.2 mL / L;

[0019] Preferably, in S2, the water bath temperature is 30-40°C, and the shaking speed of the shaker is 150-200 rpm;

[0020] Preferably, in S3, the centrifuge speed is 4000-5000 rpm, and the centrifugation time is 8-12 min;

[0021] Preferably, in S4, the amount of the bacterial solution added is 3%-5% of the wall material solution;

[0022] The addition of glycerol destroys the crystalline area of ​​starch, weakens the hydrogen bonding forces within and between the native starch molecules, and improves the thermoplasticity of starch.

[0023] Amylose is a single helical chain with a hydrophobic inner cavity and a hydrophilic outer cavity. Stearic acid is added to fill the inner cavity to form an amylose-lipid complex, which forms a hydrophobic film on the surface of the microcapsule, increases the hydrophobicity of the microcapsule, slows the hydration of the starch granule surface, hinders the entry of water molecules into the starch granules, slows down the release of the loaded bacterial agent, and has a good protective effect.

[0024] The present invention also provides a method for preparing an environmentally friendly biological liquid fertilizer, which specifically comprises the following steps:

[0025] Amino acid fertilizer, potassium humate and glucose are added to 95°C hot water and stirred to dissolve. After mixing evenly, the mixture is kept warm in a water bath for 5-7 minutes to remove bacteria and cooled. After cooling, impurities are filtered out and microcapsule bacterial agent liquid fertilizer is added and mixed evenly to obtain an environmentally friendly biological liquid fertilizer.

[0026] The beneficial effects achieved by the present invention are as follows: a single bacterial agent is coated with a composite wall material composed of corn starch, glycerol and sodium lignin sulfonate, starch particles on the surface of the microcapsule are modified with stearic acid to prepare hydrophobic single bacterial agent microcapsules, which are dispersed in an inorganic fertilizer solution to combine multiple bacterial agents, and amino acids and carbon sources are supplemented to prepare a composite biological liquid fertilizer, thereby avoiding microbial antagonism, reducing the impact of a high-concentration salt solution environment on microorganisms, protecting the activity of the microbial agent, and achieving the technical effects of high microbial survival rate and integrated water and fertilizer; corn starch is renewable and biodegradable, and the degradation products are carbon dioxide and water, which can be recycled through photosynthesis without polluting the environment, and has the advantages of being easily available, cheap, and non-toxic; the corn starch decomposed by the microcapsules can be used as a carbon source by the microbial agent, further improving the survival of the agent. rate; glycerol molecules are small and have good permeability, form strong hydrogen bonds with hydroxyl groups, and have good compatibility with starch molecules. The addition of glycerol can destroy the crystalline area of ​​starch, improve the thermoplasticity of starch, increase the density of microcapsules, and delay the aging and decomposition of microcapsules; the benzene ring structure of sodium lignin sulfonate makes it act as a reinforcing agent in the material to increase the strength of microcapsules. It can hydrogen bond with starch to destroy the intermolecular force, act as a plasticizer to enhance the toughness of microcapsules, and there are hydrogen bonds between starch and glycerol molecules and dipole-dipole effects between polar molecules to form a compact structure; stearic acid can fill the inner cavity of amylose and form a starch-lipid complex with starch on the surface of microcapsules, slowing down starch hydration and improving its stability. It forms a hydrophobic film on the surface of microcapsules to hinder the entry of water molecules and protect the bacterial agent. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] FIG1 is a graph showing the results of a 90-day shelf life experiment on fertilizers of Examples 1-3 and Comparative Examples 1-2;

[0028] FIG2 is a graph showing the fresh weight of cucumber plants in Examples 1-3, Comparative Examples 1-2, and a blank group;

[0029] FIG3 is a graph showing the plant height of cucumbers in Examples 1-3, Comparative Examples 1-2, and a blank group;

[0030] FIG4 is a graph showing the root length of cucumber plants in Examples 1-3, Comparative Examples 1-2, and a blank group;

[0031] FIG5 is a graph showing the results of the stem diameter of cucumber plants in Examples 1-3, Comparative Examples 1-2, and a blank group;

[0032] FIG6 is a graph showing the leaf area of ​​cucumber plants in Examples 1-3, Comparative Examples 1-2, and a blank group;

[0033] FIG7 is a graph showing the SPAD results of chlorophyll content in cucumber plants of Examples 1-3, Comparative Examples 1-2, and a blank group.

[0034] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those familiar to those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein can be applied to the present invention. The preferred embodiments and materials described herein are for illustrative purposes only and are not intended to limit the scope of this application.

[0037] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the test materials and test strains used in the following examples, unless otherwise specified, are purchased from commercial channels.

[0038] Tween-80 (CasNo: 9005-65-6) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number A43489;

[0039] Corn starch (CasNo: 9005-25-8) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number S116028;

[0040] Sodium lignin sulfonate (CasNo: 8061-51-6) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number A87075;

[0041] Glycerol (CasNo: 56-81-5) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number G1100;

[0042] Stearic acid (CasNo: 57-11-4) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number 174490010;

[0043] Potassium hydrogen phosphate (CasNo: 7758-11-4) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number A46041;

[0044] Urea (CasNo: 57-13-6) was purchased from Beijing Yinuokai Technology Co., Ltd., catalog number A93228;

[0045] Bacillus subtilis, biological source: China Industrial Microorganism Culture Collection Center, strain number: CICC25064;

[0046] Bacillus licheniformis, biological source: China Industrial Microorganism Culture Collection Center, strain number: CICC10103;

[0047] Bacillus pumilus, biological source: China Industrial Microorganism Culture Collection Center, strain number: CICC10813;

[0048] Bacillus megaterium, biological source: China Industrial Microorganism Culture Collection Center, strain number: CICC10024;

[0049] Bacillus amyloliquefaciens, biological source: China Industrial Microorganism Culture Collection Center, strain number: CICC10026;

[0050] Bacillus jelly, biological source is China Industrial Microorganism Culture Collection Center, strain number is CICC23575.

[0051] Example 1

[0052] An environmentally friendly biological liquid fertilizer comprises the following components in parts by weight: 105 parts of microcapsule bacterial agent liquid fertilizer, 15 parts of amino acid fertilizer, 12 parts of glucose, 10 parts of potassium humate, and 500 parts of water.

[0053] Preferably, the microcapsule bacterial agent liquid fertilizer comprises the following components in parts by weight: 0.35 parts of bacterial powder, 60 parts of corn starch, 8 parts of sodium lignin sulfonate, 19 parts of glycerol, 1.2 parts of stearic acid, 150 parts of dipotassium hydrogen phosphate, 250 parts of urea, and 1200 parts of water.

[0054] Preferably, the method for preparing the microcapsule bacterial agent liquid fertilizer specifically comprises the following steps:

[0055] S1. Add Bacillus megaterium, Bacillus mucilaginosus, Bacillus subtilis, and Bacillus licheniformis / breve / amyloliquefaciens to sterile water, add Tween-80 dropwise at a rate of 3.2 mL / L, incubate in a 70°C water bath for 25 min, then rapidly cool to 40°C, and shake on a shaker at 300 rpm for 40 min to obtain four bacterial suspensions.

[0056] S2. The bacterial suspension obtained in S1 was inoculated into a liquid culture medium with a pH of 7 and a mass ratio of peptone: beef extract powder: NaCl: agar: distilled water of 5:3:5:15:1000, and the culture medium was shaken at 200 rpm in a 40°C water bath for 12 h to obtain a fermentation seed solution.

[0057] S3: The fermentation seed liquid obtained in S2 was inoculated into a new liquid culture medium at a concentration of 1% by volume, and the culture was shaken on a shaker at a speed of 180 rpm for 40 h. The culture was centrifuged at a speed of 5000 rpm for 12 min, and the culture was resuspended in liquid culture medium to obtain an effective viable cell count of ≥ 4 × 10 10 CFU / mL of bacterial solution;

[0058] S4, corn starch and water were mixed in a mass ratio of 1:12, sodium lignin sulfonate and glycerol were added in sequence, and stirred to obtain a wall material solution, 5% by mass of the wall material solution of the bacterial solution obtained in S3 was added, and the mixture was stirred in a water bath at 50°C at a speed of 300 rpm for 20 minutes to obtain a microcapsule bacterial agent, and then stearic acid was added, and the mixture was stirred in a water bath for 1 hour, cooled to room temperature, and spray-dried to obtain a microcapsule bacterial agent;

[0059] S5, adding dipotassium hydrogen phosphate and urea to water, heating and stirring until completely dissolved, to obtain an inorganic fertilizer solution;

[0060] S6. Evenly mix the mixed microcapsule bacterial agent obtained in S4 and the inorganic fertilizer solution obtained in S5 to obtain an effective viable bacterial count of ≥4×10 9 CFU / mL of microencapsulated bacterial liquid fertilizer.

[0061] The present invention also provides a method for preparing an environmentally friendly biological liquid fertilizer, which specifically comprises the following steps:

[0062] Amino acid fertilizer, potassium humate and glucose are added to 95°C hot water and stirred to dissolve. After mixing evenly, the mixture is kept warm in a water bath for 7 minutes to remove bacteria and cooled. After cooling, impurities are filtered out and microcapsule bacterial agent liquid fertilizer is added and mixed evenly to obtain an environmentally friendly biological liquid fertilizer.

[0063] Example 2

[0064] The invention discloses an environmentally friendly biological liquid fertilizer comprising the following components in parts by weight: 95 parts of microcapsule bacterial agent liquid fertilizer, 10 parts of amino acid fertilizer, 8 parts of glucose, 7 parts of potassium humate and 400 parts of water.

[0065] Preferably, the microcapsule bacterial agent liquid fertilizer comprises the following components in parts by weight: 0.25 parts of bacterial powder, 50 parts of corn starch, 6 parts of sodium lignin sulfonate, 16 parts of glycerol, 0.8 parts of stearic acid, 100 parts of dipotassium hydrogen phosphate, 200 parts of urea, and 1000 parts of water.

[0066] Preferably, the method for preparing the microcapsule bacterial agent liquid fertilizer specifically comprises the following steps:

[0067] S1. Add Bacillus megaterium, Bacillus mucilaginosus, Bacillus subtilis, and Bacillus licheniformis / breve / amyloliquefaciens to sterile water, add Tween-80 dropwise at a rate of 2.2 mL / L, and place in a 60°C water bath for 15 min. After that, quickly cool to 30°C and shake on a shaker at 200 rpm for 30 min to obtain four bacterial suspensions.

[0068] S2. The bacterial suspension obtained in S1 was inoculated into a liquid culture medium with a pH of 7 and a mass ratio of peptone: beef extract powder: NaCl: agar: distilled water of 5:3:5:15:1000, and the culture medium was placed on a shaker at 150 rpm in a 30°C water bath for 10 h to obtain a fermentation seed solution;

[0069] S3: The fermentation seed liquid obtained in S2 was inoculated into a new liquid culture medium at a concentration of 1% by volume, and the culture was shaken on a shaker at a speed of 150 rpm for 30 h. The culture was centrifuged at a speed of 4000 rpm for 8 min, and the culture was resuspended in liquid culture medium to obtain an effective viable cell count of ≥4×10 10 CFU / mL of bacterial solution;

[0070] S4, corn starch and water were mixed in a mass ratio of 1:9, sodium lignin sulfonate and glycerol were added in sequence, and stirred to obtain a wall material solution, 3% by mass of the wall material solution of the bacterial solution obtained in S3 was added, and the mixture was stirred in a water bath at 50°C at a speed of 300 rpm for 20 min to obtain a microcapsule bacterial agent, and then stearic acid was added, and the mixture was stirred in a water bath for 1 h, cooled to room temperature, and spray-dried to obtain a microcapsule bacterial agent;

[0071] S5, adding dipotassium hydrogen phosphate and urea to water, heating and stirring until completely dissolved, to obtain an inorganic fertilizer solution;

[0072] S6. Evenly mix the mixed microcapsule bacterial agent obtained in S4 and the inorganic fertilizer solution obtained in S5 to obtain an effective viable bacterial count of ≥4×10 9 CFU / mL of microencapsulated bacterial liquid fertilizer.

[0073] The present invention also provides a method for preparing an environmentally friendly biological liquid fertilizer, which is the same as that of Example 1.

[0074] Example 3

[0075] An environmentally friendly biological liquid fertilizer comprises the following components in parts by weight: 100 parts of microcapsule bacterial agent liquid fertilizer, 12 parts of amino acid fertilizer, 10 parts of glucose, 8 parts of potassium humate, and 450 parts of water.

[0076] Preferably, the microcapsule bacterial agent liquid fertilizer comprises the following components in parts by weight: 0.3 parts of bacterial powder, 55 parts of corn starch, 7 parts of sodium lignin sulfonate, 18 parts of glycerol, 1 part of stearic acid, 120 parts of dipotassium hydrogen phosphate, 230 parts of urea, and 1100 parts of water.

[0077] Preferably, the method for preparing the microcapsule bacterial agent liquid fertilizer specifically comprises the following steps:

[0078] S1. Add Bacillus megaterium, Bacillus mucilaginosus, Bacillus subtilis, and Bacillus licheniformis / breve / amyloliquefaciens to sterile water, add Tween-80 dropwise at a rate of 2.8 mL / L, incubate in a 65°C water bath for 20 min, then rapidly cool to 35°C, and shake on a shaker at 250 rpm for 35 min to obtain four bacterial suspensions.

[0079] S2. The bacterial suspension obtained in S1 was inoculated into a liquid culture medium with a pH of 7 and a mass ratio of peptone: beef extract powder: NaCl: agar: distilled water of 5:3:5:15:1000, and the culture medium was placed on a shaker at 160 rpm in a 35°C water bath for 11 h to obtain a fermentation seed liquid;

[0080] S3: The fermentation seed liquid obtained in S2 was inoculated into a new liquid culture medium at a concentration of 1% by volume, and the culture was shaken on a shaker at 165 rpm for 35 h. The culture was centrifuged at 4500 rpm for 10 min, and the culture was resuspended in liquid culture medium to obtain an effective viable cell count of ≥ 4 × 10 10 CFU / mL of bacterial solution;

[0081] S4, corn starch and water were mixed in a mass ratio of 1:10, sodium lignin sulfonate and glycerol were added in sequence, and stirred to obtain a wall material solution, 4% by mass of the wall material solution of the bacterial solution obtained in S3 was added, and the mixture was stirred in a water bath at 50°C at a speed of 300 rpm for 20 minutes to obtain a microcapsule bacterial agent, and then stearic acid was added, and the mixture was stirred in a water bath for 1 hour, cooled to room temperature, and spray-dried to obtain a microcapsule bacterial agent;

[0082] S5, adding dipotassium hydrogen phosphate and urea to water, heating and stirring until completely dissolved, to obtain an inorganic fertilizer solution;

[0083] S6. Evenly mix the mixed microcapsule bacterial agent obtained in S4 and the inorganic fertilizer solution obtained in S5 to obtain an effective viable bacterial count of ≥4×10 9 CFU / mL of microencapsulated bacterial liquid fertilizer.

[0084] The present invention also provides a method for preparing an environmentally friendly biological liquid fertilizer, which is the same as that of Example 1.

[0085] Comparative Example 1

[0086] This comparative example provides a fertilizer, which differs from Example 3 only in that all components do not contain stearic acid, and the remaining components and component contents are the same as those in Example 1.

[0087] Comparative Example 2

[0088] This comparative example provides a fertilizer, which differs from Example 3 only in that the corn starch-sodium lignin sulfonate-glycerol microcapsule component is not included in all components, and the remaining components and component contents are the same as those in Example 1.

[0089] Experimental example

[0090] 1. Shelf life experiment

[0091] Take 200 mL of the fertilizer obtained in Example 1-3 and Comparative Example 1-2, respectively, package them in polyethylene bags, store them in dark conditions, and test the effective viable count of the prepared fertilizers every 10 days for a total of 10 measurements. The effective viable count measured at 0 d is taken as the initial viable count, and the survival rate is calculated using the following formula.

[0092] Survival rate = effective viable bacteria count / initial viable bacteria count × 100%.

[0093] 2. Cucumber Planting Experiment

[0094] Select cucumber seeds with full grains and uniform size, sterilize them with 75% alcohol for 15 seconds, rinse them with sterile water 5 times, and culture them in an incubator with a temperature of 28°C, a light intensity of 20,000 lx, and a daily light duration of 16 hours. When the seed buds are 1.5 cm long, transplant the seedlings to the experimental field. The soil type of the experimental field is red soil, and the cultivation area is 5 m 2 The plant spacing was 0.2 m, and there were 6 groups in total. 200-fold diluted Examples 1-3 and Comparative Examples 1-2 were used as experimental groups, and sterile water was used as a blank group for drip irrigation. Drip irrigation was performed once every 7 days. After 35 days, fresh weight, plant height, root length, stem diameter, leaf area, and chlorophyll content SPAD were measured.

[0095] Result Analysis

[0096] Figure 1 is a graph showing the results of a 90-day shelf life experiment on fertilizers of Examples 1-3 and Comparative Example 1-2; as shown in the figure, the survival rates of Examples 1-3 and Comparative Example 1-3 on the 90th day were 62.32%, 60.16%, 63.31%, 49.65% and 37.65%, respectively. The survival rate of Example 1-3 was significantly higher than that of Comparative Example 1, indicating that the addition of stearic acid components in the microcapsules helped to improve the survival rate of the strains. The survival rate of Example 1-3 was significantly higher than that of Comparative Example 2, indicating that the use of the microcapsule components in the examples helped to improve the survival rate of the strains; corn starch It has a polyhydroxy structure that is sensitive to moist environments. Hydrogen bonds are formed between glycerol and starch molecules to reduce starch agglomeration. Sodium lignin sulfonate destroys the interaction between starch molecules and destroys the crystalline area. Hydrogen bonding and dipole-dipole effects make corn starch-glycerol-sodium lignin sulfonate highly compatible and tightly combined. The composite wall material has good density. Stearic acid molecules and amylose form a thin film on the surface. The microcapsules are hydrophobic and do not affect the internal bacterial agent. They play a protective role to prevent microbial antagonism. After decomposition, they provide a carbon source, which effectively improves the survival rate of bacteria in liquid fertilizers.

[0097] Figure 2 is a graph showing the fresh weight of cucumber plants in Examples 1-3, Comparative Example 1-2 and a blank group; Figure 3 is a graph showing the plant height of cucumber plants in Examples 1-3, Comparative Example 1-2 and a blank group; Figure 4 is a graph showing the root length of cucumber plants in Examples 1-3, Comparative Example 1-2 and a blank group; Figure 5 is a graph showing the stem diameter of cucumber plants in Examples 1-3, Comparative Example 1-2 and a blank group; Figure 6 is a graph showing the leaf area of ​​cucumber plants in Examples 1-3, Comparative Example 1-2 and a blank group; Figure 7 is a graph showing the chlorophyll content SPAD of cucumber plants in Examples 1-3, Comparative Example 1-2 and a blank group; as shown in the figure, after culturing for 35 days, the fresh weights of the cucumber plants obtained in Examples 1-3, Comparative Example 1-2 and a blank group were 66.54 g, 65.93 g, 67.13 g and 59.81 g, respectively. g, 55.41 g, 51.26 g; the heights of the cucumber plants obtained in Example 1-3, Ratio 1-2, and the blank group were 104.6 cm, 103.8 cm, 105.4 cm, 93.1 cm, 86.7 cm, and 75.2 cm, respectively; the root lengths of the cucumber plants obtained in Example 1-3, Ratio 1-2, and the blank group were 36.3 cm, 37.1 cm, 35.5 cm, 30.6 cm, 28.1 cm, and 24.9 cm, respectively; the stem diameters of the cucumber plants obtained in Example 1-3, Ratio 1-2, and the blank group were 6.4 mm, 6.3 mm, 6.4 mm, 5.7 mm, 5.4 mm, and 5.2 mm, respectively; and the leaf areas of the cucumber plants obtained in Example 1-3, Ratio 1-2, and the blank group were 130.82 cm, respectively. 2 、129.37cm 2 、131.94cm 2 、119.16cm 2、101.84cm 2 、92.97cm 2 The chlorophyll content SPAD of the cucumber plants obtained in Examples 1-3, Comparative Example 1-2, and the blank group were 49.53, 50.28, 51.97, 42.39, 37.41, and 33.15, respectively; the fresh weight, plant height, root length, stem diameter, leaf area, and chlorophyll content SPAD of the cucumber plants obtained in Examples 1-3 were significantly higher than those in Comparative Example 1-2, and the fresh weight, plant height, and root length of the pakchoy obtained in Comparative Example 1-2 were significantly higher than those in the blank group. Example 1-3 can better promote the growth of pakchoy than Example 1-2, and the use of microcapsule components can effectively increase its yield and quality. Example 1 can better promote the growth of pakchoy than Example 2, and the use of stearic acid improves the hydrophobicity and stability of the microcapsules.

[0098] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

[0099] The present invention and its embodiments are described above. Such description is not restrictive. The drawings show only one embodiment of the present invention, and actual applications are not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, any method and embodiment similar to the technical solution without creative design shall fall within the scope of protection of the present invention.

Claims

1. An environmentally friendly biological liquid fertilizer, characterized in that: The invention comprises the following components in parts by weight: 95-105 parts of microcapsule bacterial agent liquid fertilizer, 10-15 parts of amino acid fertilizer, 8-12 parts of glucose, 7-10 parts of potassium humate and 400-500 parts of water; the microcapsule bacterial agent liquid fertilizer is a suspension formed by bacterial agent microcapsules in an inorganic fertilizer solution; the bacterial agent microcapsules are hydrophobic microcapsules prepared by encapsulating the bacterial agent with sodium lignin sulfonate-corn starch.

2. The environmentally friendly biological liquid fertilizer according to claim 1, characterized in that: The microcapsule bacterial agent liquid fertilizer comprises the following components in parts by weight: 0.25-0.35 parts of bacterial powder, 50-60 parts of corn starch, 6-8 parts of sodium lignin sulfonate, 16-19 parts of glycerol, 0.8-1.2 parts of stearic acid, 100-150 parts of dipotassium hydrogen phosphate, 200-250 parts of urea, and 1000-1200 parts of water.

3. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 2, characterized in that: The method specifically comprises the following steps: adding amino acid fertilizer, potassium humate and glucose into 95°C hot water and stirring to dissolve, mixing evenly, keeping warm in a water bath for 5-7 minutes to remove miscellaneous bacteria, cooling, filtering out impurities after cooling, adding microcapsule bacterial agent liquid fertilizer and mixing evenly to obtain environmentally friendly biological liquid fertilizer.

4. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 3, characterized in that: The preparation method of microcapsule bacterial agent liquid fertilizer specifically comprises the following steps: S1. Take bacterial powder and add it to sterile water, add Tween-80, cool it to 30-40℃ after 15-25min in a 60-70℃ water bath, and shake it on a shaker at 200-300rpm for 30-40min to obtain a bacterial suspension; S2, inoculating the bacterial suspension obtained in S1 into a liquid culture medium with a pH of 7 and a mass ratio of peptone: beef extract powder: NaCl: agar: distilled water of 5:3:5:15:1000, and placing it on a shaker in a water bath for 10-12 hours to obtain a fermentation seed liquid; S3, inoculate the fermentation seed liquid obtained in S2 into a new liquid culture medium at a concentration of 1% by volume, place it on a shaker at a speed of 150-180 rpm for 30-40 hours, centrifuge it in a centrifuge, resuspend it in liquid culture medium, and obtain an effective viable cell count of ≥4×10 10 CFU / mL of bacterial solution; S4, corn starch and water are mixed evenly at a mass ratio of 1:9-12, sodium lignin sulfonate and glycerol are added in sequence, stirred evenly to obtain a wall material solution, the bacterial solution obtained in S3 is added, and stirred evenly in a water bath at 50°C at a speed of 300 rpm for 20 min, then stearic acid is added, stirring is continued in a water bath for 1 h, cooled to room temperature, and spray-dried to obtain a microcapsule bacterial agent; S5, adding dipotassium hydrogen phosphate and urea into water, heating and stirring until completely dissolved, to obtain an inorganic fertilizer solution; S6, mix the mixed microcapsule bacterial agent obtained in S4 and the inorganic fertilizer solution obtained in S5 evenly to obtain an effective viable bacterial count ≥ 4×10 9 CFU / mL of microencapsulated bacterial liquid fertilizer.

5. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 4, characterized in that: In S1, the bacterial powder is one or more of Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus megaterium, Bacillus amyloliquefaciens, and Bacillus gelatinosa.

6. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 5, characterized in that: In S1, the amount of Tween-80 added to water is 2.2-3.2 mL / L.

7. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 6, characterized in that: In S2, the water bath temperature is 30-40°C, and the shaking speed of the shaker is 150-200 rpm.

8. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 7, characterized in that: In S3, the centrifuge speed is 4000-5000 rpm, and the centrifugation time is 8-12 min.

9. The method for preparing the environmentally friendly biological liquid fertilizer according to claim 8, characterized in that: In S4, the amount of the bacterial solution added is 3%-5% by mass of the wall material solution.

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