Microfluidic chip for analyte detection

Abstract

Disclosed in the present invention is a microfluidic chip for analyte detection, comprising a chip body, a pressure applying cover body, and a gas discharging and solution blocking film. A flow distribution cavity, reaction cavities, and a sample loading cavity are formed in the chip body; the pressure applying cover body is connected to the sample loading cavity; the sample loading cavity is communicated with the flow distribution cavity by means of a sample loading flow channel, the flow distribution cavity is communicated with each reaction cavity by means of a solution distribution flow channel, and the reaction cavity is communicated with a gas discharging hole by means of a gas discharging flow channel; the gas discharging and solution blocking film covers gas outlets of the gas discharging holes; the pressure applying cover body comprises an air bag, and the sum of the volumes of the sample loading flow channel, the solution distribution flow channels, the gas discharging flow channels, the gas discharging holes, and the flow distribution cavity is larger than the volume of the air bag; and when the air bag deforms, a solution flows into the reaction cavities from the sample loading cavity. According to the present invention, pressure is increased in the sample loading cavity by means of the air bag or a rubber plug, and then the solution is pushed to enter the reaction cavities, so that a driving method in the prior art is replaced, and the production cost is reduced.

Description

Microfluidic chips for analyte detection Technical Field

[0001] The present invention relates to the field of microfluidic detection technology, in particular to a microfluidic chip for analyte detection. Background Art

[0002] Analyte detection in lab-on-a-chip (LOC) devices is an important detection technology in fields such as medicine and analytical chemistry. Microfluidic chips are one of the more important detection devices in LOC detection technology. In existing microfluidic chips, the flow channels are typically only 100 nanometers to 100 microns in diameter. Analytes must flow through the channels into small diversion chambers, requiring highly sophisticated instruments to generate a driving force to propel the analyte flow. This driving force can be generated by positive or negative pressure, centrifugal force, magnetic force, or charge adsorption. Instruments that can meet these precision requirements are typically costly to produce and difficult to mass-produce. Furthermore, during analyte delivery in existing microfluidic chips, bubbles or impurities in the sample can clog the extremely small flow channels, affecting the proper performance of the test. Technical issues

[0003] The technical problem to be solved by the present invention is to provide a microfluidic chip for analyte detection, which reduces the accuracy requirements for detection instruments and reduces the probability of flow channel blockage. Technical Solutions

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0005] A microfluidic chip for analyte detection, comprising a chip body, a pressure-applying cover, and an exhaust liquid-blocking membrane;

[0006] The chip body is provided with a diversion chamber, a reaction chamber and a sample addition chamber;

[0007] The pressure cover is connected to the sample adding chamber;

[0008] The sample adding chamber is connected to the diversion chamber via a sample adding channel, the diversion chamber is connected to the reaction chamber via a liquid separation channel, and the reaction chamber is connected to the exhaust hole via an exhaust channel;

[0009] The exhaust liquid-blocking film covers the exhaust outlet of the exhaust hole;

[0010] The pressure cover includes an airbag, and the sum of the volumes of the sample addition channel, the liquid separation channel, the exhaust channel, the exhaust hole, and the diversion cavity is greater than the volume of the airbag;

[0011] When the air bag is deformed, the solution flows from the sample adding chamber into the reaction chamber.

[0012] In order to solve the above technical problems, another technical solution adopted by the present invention is:

[0013] A microfluidic chip for analyte detection, comprising a chip body, a pressure-applying cover, and an exhaust liquid-blocking membrane;

[0014] The chip body is provided with a diversion chamber, a reaction chamber and a sample addition chamber;

[0015] The sample addition chamber is connected to the reaction chamber via a sample addition channel, and the reaction chamber is connected to the waste liquid hole via an overflow channel;

[0016] The exhaust liquid blocking film covers the exhaust end of the waste liquid hole;

[0017] The pressure cover body has a rubber plug;

[0018] The sum of the volumes of the sample addition channel, the liquid separation channel, the exhaust channel, the exhaust hole, and the diversion cavity is greater than the volume of the rubber stopper;

[0019] When the rubber plug is inserted into the sample adding cavity, the solution flows from the sample adding cavity into the reaction cavity, and the excess solution flows from the reaction cavity into the waste liquid hole. Beneficial effects

[0020] The beneficial effects of the present invention are as follows: the present invention is connected to the sample loading chamber by a pressure-applying cover body, and the air pressure inside the sample loading chamber is changed by applying pressure to the air bag or embedding the plug into the sample loading chamber, so as to push the solution to flow from the sample loading chamber through the sample loading channel, the diversion chamber and the liquid separation channel into the reaction chamber in sequence, or push the solution to flow from the sample loading chamber through the sample loading channel into the reaction chamber in sequence. In order to ensure that all solutions can flow into the reaction chamber and stay in the reaction chamber, the volume of the air bag and the plug should be less than the sum of the volumes of all the channels and cavities, so that the solution fills the reaction chamber and ensures the accuracy of the test results. Compared with the prior art, the present invention can make the solution flow into the corresponding reaction chamber without the need for a high-precision instrument to apply a driving force to the sample loading chamber, and due to the provision of the overflow hole, the exhaust hole and the exhaust liquid-blocking membrane, the excess gas generated during the sample loading process can be discharged, thus avoiding blockage and greatly reducing the use cost of the microfluidic chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] FIG1 is a schematic structural diagram of a microfluidic chip for analyte detection in Example 1 of the present invention;

[0022] FIG2 is an exploded view of a microfluidic chip for analyte detection in Example 1 of the present invention;

[0023] FIG3 is a second structural diagram of a microfluidic chip for analyte detection in Example 1 of the present invention;

[0024] FIG4 is a cross-sectional view of a microfluidic chip for analyte detection in Example 1 of the present invention;

[0025] FIG5 is a side view of FIG4;

[0026] FIG6 is a schematic structural diagram of a microfluidic chip for analyte detection in Example 2 of the present invention;

[0027] FIG7 is a schematic diagram of a partial structure of a microfluidic chip for analyte detection in Example 2 of the present invention;

[0028] FIG8 is an exploded view of a microfluidic chip for analyte detection in Example 2 of the present invention;

[0029] FIG9 is a cross-sectional view of a microfluidic chip for analyte detection in Example 2 of the present invention;

[0030] FIG10 is a schematic diagram of a partial structure of a microfluidic chip for analyte detection in Example 3 of the present invention;

[0031] FIG11 is a second schematic diagram of the partial structure of the microfluidic chip for analyte detection in Example 3 of the present invention;

[0032] FIG12 is a cross-sectional view of a microfluidic chip for analyte detection in Example 3 of the present invention;

[0033] FIG13 is an exploded view of the microfluidic chip for analyte detection in Example 3 of the present invention;

[0034] FIG14 is a second exploded view of the microfluidic chip for analyte detection in Example 3 of the present invention.

[0035] Description of labels:

[0036] 1. Chip body; 11. Diversion chamber; 12. Reaction chamber; 13. Sample addition chamber; 14. Sample addition channel; 15. Liquid separation channel; 16. Exhaust channel; 17. Exhaust hole; 18. Limiting groove; 19. Overflow channel; 191. Waste liquid hole; 101. First side; 102. Second side;

[0037] 2. Pressure cover; 21. Cover body; 22. Air bag; 23. Rubber plug;

[0038] 3. Exhaust liquid barrier film; 4. Baffle; 5. Sealing film; 6. Bump. Modes for Carrying Out the Invention

[0039] To illustrate the technical content, achieved objectives and effects of the present invention in detail, the following description is given in conjunction with the embodiments and accompanying drawings.

[0040] The main inventive concept of the present invention is to apply pressure to the sample addition chamber through an air bag or a rubber plug to generate a driving force on the solution, and to limit the volume relationship between the air bag and the rubber plug and all flow channels and all cavities to ensure that the driving force generated by the air bag or the rubber plug on the sample addition chamber can enable the solution to be delivered to the reaction chamber.

[0041] Please refer to Figures 1 to 14. In one of the technical solutions, a microfluidic chip for analyte detection is provided, including a chip body 1, a pressure cover 2 and an exhaust liquid-blocking membrane 3; the chip body 1 is provided with a diverter chamber 11, a reaction chamber 12 and a sample adding chamber 13; the pressure cover 2 is connected to the sample adding chamber 13; the sample adding chamber 13 is connected to the diverter chamber 11 through a sample adding channel 14, the diverter chamber 11 is connected to the reaction chamber 12 through a liquid separation channel 15, and the reaction chamber 12 is connected to the exhaust hole 17 through an exhaust channel 16; the exhaust liquid-blocking membrane 3 covers the outlet of the exhaust hole 17; the pressure cover 2 includes a coaxially arranged cover body 21 and an air bag 22, and the sum of the volumes of the sample adding channel 14, the liquid separation channel 15, the exhaust channel 16, the exhaust hole 17 and the diverter chamber 11 is greater than the volume of the air bag 22; when the air bag 22 is deformed, the solution flows from the sample adding chamber 13 into the reaction chamber 12. Specifically, the volume of the airbag 22 is set as the first volume, and the sum of the volumes of the sample addition channel 14, the liquid separation channel 15, the exhaust channel 16, the exhaust hole 17 and the diversion cavity 11 is set as the second volume. The difference between the first volume and the second volume is 80 mm. 3 ~ 160 mm 3 , optional, the difference is 80 mm 3 , 90 mm 3 , 100 mm 3 , 110 mm 3 , 120 mm 3 , 130 mm 3 , 140 mm 3 , 150 mm 3 or 160 mm 3 As a preference, the difference is 120 mm 3 The pressure cover 2 is threadably connected to the sample loading chamber 13 .

[0042] It is understandable that when the pressure cover 2 is not connected to the sample loading chamber 13, the solution will rely on gravity to flow into the sample loading channel. However, the present invention connects the pressure cover 2 to the sample loading chamber 13, and by applying pressure to the airbag 22 or inserting the rubber plug 23 into the sample loading chamber 13, the air pressure inside the sample loading chamber 13 changes, thereby pushing the solution to flow from the sample loading chamber 13 through the sample loading channel 14, the diversion chamber 11, and the liquid separation channel 15 into the reaction chamber 12 in sequence. That is, the pressure cover 2 plays a role in assisting the solution to enter the sample loading chamber 13. To ensure that all solutions can flow into the reaction chamber 12 and stay in the reaction chamber 12, the airbag 22 should be smaller than the sum of the volumes of all the channels and cavities, so that the reaction liquid fills the reaction chamber 12 and ensures the accuracy of the test results. Compared with the prior art, the present invention can make the solution flow into the corresponding reaction chamber 12 without the need for high-precision instruments to apply driving force to the sample addition chamber 13. In addition, due to the arrangement of the overflow hole, the exhaust hole 17 and the exhaust liquid-blocking membrane 3, the excess gas generated during the sample addition process can be discharged, avoiding blockage and greatly reducing the use cost of the microfluidic chip.

[0043] In some embodiments, there are at least three reaction chambers 12 and at least three exhaust holes 17. All reaction chambers 12 are evenly distributed around the diversion chamber 11, and all exhaust holes 17 are evenly distributed around the outside of the reaction chambers 12. Optionally, to improve the accuracy of the test results, the number of reaction chambers 12 should be as large as possible, so eight, nine, or more reaction chambers 12 and exhaust holes 17 are provided. Providing exhaust holes 17 around the outside of the reaction chambers 12 maximizes the number of reaction chambers 12 and exhaust holes 17.

[0044] In some embodiments, the chip body 1 has a first side 101 and a second side 102 along the thickness direction of the chip body 1. The first side 101 is provided with a limiting groove 18 for accommodating the exhaust liquid barrier film 3. The exhaust hole 17 has an outlet located within the limiting groove 18, and the limiting groove 18 is covered with a baffle 4. Along the thickness direction of the chip body 1, the exhaust liquid barrier film 3 is sandwiched between the baffle 4 and the inner wall of the limiting groove 18. The provision of the baffle 4 serves to prevent dust and protect the exhaust liquid barrier film. The exhaust hole 17 axially extends through the first side 101 and the second side 102 of the chip body 1.

[0045] In some embodiments, the sample addition chamber 13 is eccentrically disposed relative to the diverter chamber 11 and is disposed near the edge of the chip body 1. The eccentricity of the sample addition chamber 13 relative to the diverter chamber 11 allows the sample addition channel 14 to have a larger space for distribution, providing sufficient buffer space when the solution flows into the diverter chamber 11, thereby preventing the solution from directly rushing into the diverter chamber 11 and generating a large number of bubbles that would affect the detection results.

[0046] In some embodiments, a sealing film 5 is further included, and the sample addition channel 14, exhaust channel 16, liquid separation channel 15, diversion chamber 11, and reaction chamber 12 are all opened on the second side 102 of the chip body 1. The sealing film 5 covers the second side 102 of the chip body 1, so that the sample addition channel 14, exhaust channel 16, liquid separation channel 15, diversion chamber 11, and reaction chamber 12 are sealed. Since the corresponding reagent needs to be added to the reaction chamber 12 to react with the solution, the above-mentioned channels and chambers are all arranged on the second side 102 of the chip body 1 to facilitate the addition of the reagent to the reaction chamber 12, and are covered by the sealing film 5 to form a sealed cavity, which is convenient for reducing the difficulty of production.

[0047] In some embodiments, the length of the liquid separation channel 15 is greater than the length of the exhaust channel 16 , so that there is sufficient buffer space when the reactants flow into the reaction chamber 12 , thereby reducing the generation of bubbles.

[0048] In some embodiments, the depth and / or width of the sample addition channel 14 and the liquid separation channel 15 is 100 μm to 3000 μm. When the channel depth or width is large enough, it can accommodate more reactants and can buffer the reactants, reducing the generation of bubbles. Optionally, the depth and / or width is 100μm, 200μm, 300μm, 400μm, 500μm, 600μm, 700μm, 800μm, 900μm, 1000μm, 1100μm, 1200μm, 1300μm, 1400μm, 1500μm, 1600μm, 1700μm, 1800μm, 1900μm, 2000μm, 2100μm, 2200μm, 2300μm, 2400μm, 2500μm, 2600μm, 2700μm, 2800μm, 2900μm or 3000μm.

[0049] In another technical solution, a microfluidic chip for analyte detection is provided, comprising a chip body 1, a pressure cover 2 and an exhaust liquid-blocking membrane 3; the chip body 1 is provided with a diversion chamber 11, a reaction chamber 12 and a sample addition chamber 13; the sample addition chamber 13 is connected to the reaction chamber 12 through a sample addition channel 14, and the reaction chamber 12 is connected to the waste liquid hole 191 through an overflow channel 19; the exhaust liquid-blocking membrane 3 covers the gas outlet end of the waste liquid hole 191; the pressure cover 2 comprises a cover body 21 and a plug 23; the sum of the volumes of the sample addition channel 14, the liquid separation channel 15, the exhaust channel 16, the exhaust hole 17 and the diversion chamber 11 is greater than the volume of the plug 23; when the plug 23 is embedded in the sample addition chamber 13, the solution flows from the sample addition chamber 13 into the reaction chamber 12, and the excess solution flows from the reaction chamber 12 into the waste liquid hole 191.

[0050] It is understandable that the present invention is connected to the sample loading chamber 13 through the pressure cover 2, and the air pressure inside the sample loading chamber 13 is changed by applying pressure to the air bag 22 or embedding the plug 23 into the sample loading chamber 13, so as to push the solution to flow from the sample loading chamber 13 through the sample loading channel 14 into the reaction chamber 12 in sequence. In order to ensure that the solution can flow into the reaction chamber 12 and stay in the reaction chamber 12, the volume of the plug 23 should be less than the sum of the volumes of all the flow channels and the cavity, so that the reaction liquid fills the reaction chamber 12 and ensures the accuracy of the test results. Compared with the prior art, the present invention can make the solution flow into the corresponding reaction chamber 12 without the need for a high-precision instrument to apply a driving force to the sample loading chamber 13, and due to the setting of the overflow hole, the exhaust hole 17 and the exhaust liquid-blocking membrane 3, the excess gas generated during the sample loading process can be discharged, avoiding blockage and greatly reducing the use cost of the microfluidic chip.

[0051] In some embodiments, the pressure cap 2 is threadedly connected to the sample loading chamber 13 .

[0052] In some embodiments, in the thickness direction of the chip body 1, the chip body 1 has a first side 101 and a second side 102; the overflow channel 19 is opened on the first side 101, and the sample addition channel 14 is opened on the second side 102. When the liquid level in the reaction chamber 12 is higher than the bottom surface of the overflow channel 19, the solution will enter the waste liquid hole 191 through the overflow channel 19 for storage to avoid the solution volume being too large and being retained in the sample addition chamber 13, unable to react with the reagent, and affecting the accuracy of the test results.

[0053] In some embodiments, the material of the exhaust liquid blocking membrane 3 is polytetrafluoroethylene or polydimethylsiloxane. The exhaust liquid blocking membrane 3 made of this material has a good exhaust liquid blocking effect, which can ensure that the gas is discharged, keep the air pressure in the flow channel balanced, ensure that the solution can flow into the reaction chamber 12, and prevent the solution from flowing out of the exhaust hole 17.

[0054] In some embodiments, the microfluidic chip is used to detect at least one of bacteria, viruses, fungi, parasites, DNA, and RNA.

[0055] In some embodiments, the virus is at least one of influenza A virus, influenza B virus, respiratory syncytial virus, and SARS-CoV-2 virus.

[0056] In some embodiments, the reaction chamber 12 is filled with at least one reagent for detecting an analyte. Specifically, the reagent is in a freeze-dried state. Optionally, one of the reagents is a color developer, which is used to change the color of the solution when the analyte is mixed in the solution; one of the reagents is a fluorescent signal detector, which generates a fluorescent signal under the action of the fluorescent signal detector when the analyte is present in the solution, so that the detection instrument can obtain the fluorescent signal.

[0057] In some embodiments, in order to improve the accuracy of the detection results, the inner wall of the reaction chamber 12 is surface treated to form a frosted effect, so that the detection spot is more uniform, thereby improving the accuracy of the detection results.

[0058] In some embodiments, the sidewalls of the chip body 1 are provided with bumps 6 to facilitate positioning of the chip body 1 in a detection cavity of a detection instrument.

[0059] 1 to 5 , the first embodiment of the present invention is as follows:

[0060] A microfluidic chip for analyte detection includes a chip body 1, a pressure cover 2 and an exhaust liquid-blocking membrane 3; the chip body 1 is provided with a diverter chamber 11, a reaction chamber 12 and a sample addition chamber 13; the pressure cover 2 is connected to the sample addition chamber 13; the sample addition chamber 13 is connected to the diverter chamber 11 through a sample addition channel 14, the diverter chamber 11 is connected to the reaction chamber 12 through a liquid separation channel 15, and the reaction chamber 12 is connected to the exhaust hole 17 through an exhaust channel 16; the exhaust liquid-blocking membrane 3 covers the outlet of the exhaust hole 17; the pressure cover 2 includes a coaxially arranged cover body 21 and an air bag 22, the air bag 22 is arranged on one side of the cover body 21 in the axial direction, and the sum of the volumes of the sample addition channel 14, the liquid separation channel 15, the exhaust channel 16, the exhaust hole 17 and the diverter chamber 11 is greater than the volume of the air bag 22; when the air bag 22 is deformed, the solution flows from the sample addition chamber 13 into the reaction chamber 12. Specifically, the volume of the airbag 22 is set as the first volume, and the sum of the volumes of the sample addition channel 14, the liquid separation channel 15, the exhaust channel 16, the exhaust hole 17 and the diversion cavity 11 is set as the second volume. The difference between the first volume and the second volume is 120 mm. 3 The main body of the pressure cover 2 is threadedly connected to the sample adding chamber 13 .

[0061] In this embodiment, nine reaction chambers 12 and nine exhaust holes 17 are provided. All the reaction chambers 12 are evenly distributed around the diversion chamber 11 , and all the exhaust holes 17 are arranged around the outside of the reaction chambers 12 .

[0062] In this embodiment, in the thickness direction of the chip body 1, the chip body 1 has a first side 101 and a second side 102; the first side 101 is provided with a limiting groove 18 for accommodating the exhaust liquid-blocking film 3, the air outlet of the exhaust hole 17 is located in the limiting groove 18, and the limiting groove 18 is covered with a baffle 4. In the thickness direction of the chip body 1, the exhaust liquid-blocking film 3 is clamped between the baffle 4 and the inner wall of the limiting groove 18.

[0063] In this embodiment, the sample adding cavity 13 is eccentrically disposed relative to the diversion cavity 11 and is disposed close to the edge of the chip body 1 .

[0064] In this embodiment, a sealing film 5 is also included. The sample addition channel 14, the exhaust channel 16, the liquid separation channel 15, the diversion chamber 11 and the reaction chamber 12 are all opened on the second side 102 of the chip body 1. After the reagent is added to the reaction chamber 12, the sealing film 5 is covered on the second side 102 of the chip body 1 to seal the sample addition channel 14, the exhaust channel 16, the liquid separation channel 15, the diversion chamber 11 and the reaction chamber 12.

[0065] In this embodiment, the length of the liquid separation channel 15 is greater than that of the exhaust channel 16 , and the depth of the sample addition channel 14 and the liquid separation channel 15 is 500 μm and the width is 350 μm.

[0066] In this embodiment, the exhaust liquid blocking film 3 is made of polytetrafluoroethylene.

[0067] The working principle of this embodiment is:

[0068] After the reagents are added to the reaction chamber 12 , the second side 102 of the chip body 1 is covered by the sealing film 5 ;

[0069] Add the solution to be tested from the sample addition chamber 13, thread the pressure cover 2 to the sample addition chamber 13, press the air bag 22 of the pressure cover 2, push the solution through the sample addition channel 14 into the diversion chamber 11, and enter the corresponding reaction chamber 12 through the liquid separation channel 15 to react with the reagent in the reaction chamber 12. During this process, the airflow generated by the pressure deformation of the air bag 22 will be discharged through the exhaust channel 16 and the exhaust hole 17.

[0070] After the sample addition is completed, the microfluidic chip is placed in the corresponding detection instrument for detection.

[0071] 6 to 9 , the second embodiment of the present invention is as follows:

[0072] The difference between this embodiment and the first embodiment lies in that the number of reaction chambers 12 and exhaust holes 17 is different, and the position of the sample adding chamber 13 is different.

[0073] In this embodiment, the sample adding cavity 13 is centrally arranged relative to the length direction of the chip body 1 , that is, the axis of the sample adding cavity 13 is perpendicular to the length direction of the chip body 1 .

[0074] In this embodiment, eight reaction chambers 12 and eight exhaust holes 17 are provided, and all reaction chambers 12 and exhaust holes 17 are arranged in a straight line with equal intervals; the diversion chamber 11 is centered relative to the length direction of the chip body 1.

[0075] 10 to 14 , the third embodiment of the present invention is as follows:

[0076] The difference between this embodiment and the first embodiment is that the microfluidic chip includes a chip body 1, a pressure cover 2 and an exhaust liquid blocking membrane 3; the chip body 1 has a diversion chamber 11, a reaction chamber 12 and a sample adding chamber 13; the sample adding chamber 13 is connected to the reaction chamber 12 through a sample adding channel 14, and the reaction chamber 12 is connected to the waste liquid hole 191 through an overflow channel 19; the exhaust liquid blocking membrane 3 covers the gas outlet end of the waste liquid hole 191; the pressure cover 2 includes a cover body 21 and a rubber plug 23, and the rubber plug 23 is arranged on the cover body 21 facing the Toward one side of the sample loading chamber 13, when the cover 21 is threadedly connected to the sample loading chamber 13, the rubber plug 23 will be embedded in the sample loading chamber 13, applying pressure to the interior of the sample loading chamber 13 to promote the flow of solution; the sum of the volumes of the sample loading channel 14, the liquid separation channel 15, the exhaust channel 16, the exhaust hole 17, and the diversion chamber 11 is greater than the volume of the rubber plug 23; when the rubber plug 23 is embedded in the sample loading chamber 13, the solution flows from the sample loading chamber 13 into the reaction chamber 12, and the excess solution flows from the reaction chamber 12 into the waste liquid hole 191. In this embodiment, the volume of the rubber plug 23 refers to the change in volume of the internal cavity of the sample loading chamber 13 caused by the rubber plug 23 being embedded in the sample loading chamber 13.

[0077] In this embodiment, two limiting grooves 18 are provided, each limiting groove 18 is covered with an exhaust liquid blocking film 3, four sample loading chambers 13 are provided, and the first side 101 and the second side 102 of the chip body 1 are both provided with a sealing film 5, and the sealing film 5 located on the second side 102 covers the exhaust liquid blocking film 3.

[0078] In this embodiment, the length of the liquid separation channel 15 is greater than that of the overflow channel 19 , and the depth of the sample addition channel 14 and the liquid separation channel 15 is 500 μm and the width is 350 μm.

[0079] The working principle of this embodiment is:

[0080] Add reagents into the reaction chamber 12 and cover the first side 101 and the second side 102 of the chip body 1 with the sealing film 5 respectively;

[0081] The solution to be tested is added to the sample adding chamber 13, and the pressure cover 2 is threadedly connected to the sample adding chamber 13. During this process, the rubber plug 23 is embedded in the sample adding chamber 13, so that the air pressure inside the sample adding chamber 13 changes, generating a driving force for the solution to be tested, so that the solution flows through the sample adding channel 14 into the corresponding reaction chamber 12.

[0082] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the present invention's description and drawings, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A microfluidic chip for analyte detection, characterized in that: It includes a chip body, a pressure cover body and an exhaust liquid blocking film; The chip body is provided with a flow distribution chamber, a reaction chamber and a sample adding chamber; The pressure cover is connected to the sample adding chamber; The sample adding chamber is connected to the flow dividing chamber through a sample adding channel, the flow dividing chamber is connected to the reaction chamber through a liquid dividing channel, and the reaction chamber is connected to the exhaust hole through an exhaust channel; The exhaust liquid blocking film covers the exhaust port of the exhaust hole; The pressure cover body includes an air bag, and the sum of the volumes of the sample addition channel, the liquid separation channel, the exhaust channel, the exhaust hole and the flow separation cavity is greater than the volume of the air bag; When the air bag is deformed, the solution flows from the sample adding chamber into the reaction chamber.

2. The microfluidic chip for analyte detection according to claim 1, characterized in that: The reaction chamber and the exhaust holes are each provided with more than three; All the reaction chambers are evenly distributed around the diversion chamber, and all the exhaust holes are evenly distributed around the outside of the reaction chamber.

3. The microfluidic chip for analyte detection according to claim 1, characterized in that: In the thickness direction of the chip body, the chip body has a first side and a second side; The first side is provided with a limiting groove for accommodating the exhaust liquid blocking film, the exhaust hole's air outlet is located in the limiting groove, and a blocking piece is provided inside the limiting groove. In the thickness direction of the chip body, the exhaust liquid blocking film is clamped between the blocking piece and the limiting groove.

4. The microfluidic chip for analyte detection according to claim 1, characterized in that: The sample adding cavity is eccentrically arranged relative to the diversion cavity and is arranged close to the edge of the chip body.

5. The microfluidic chip for analyte detection according to claim 1, characterized in that: The sample adding channel, the exhaust channel, the liquid dividing channel, the flow dividing chamber and the reaction chamber are all arranged on the second side of the chip body.

6. The microfluidic chip for analyte detection according to claim 5, characterized in that: It also includes a sealing film, which covers the second side of the chip body to seal the sample addition channel, the exhaust channel, the liquid separation channel, the flow separation chamber and the reaction chamber.

7. The microfluidic chip for analyte detection according to claim 1, characterized in that: The length of the liquid separation channel is greater than the length of the exhaust channel.

8. The microfluidic chip for analyte detection according to claim 1, characterized in that: The depth and / or width of the sample addition channel and the liquid separation channel is 100 μm to 3000 μm.

9. The microfluidic chip for analyte detection according to any one of claims 1 to 8, characterized in that: The exhaust liquid blocking film is made of polytetrafluoroethylene or polydimethylsiloxane.

10. The microfluidic chip for analyte detection according to any one of claims 1 to 8, characterized in that: The microfluidic chip is used to detect at least one of bacteria, viruses, fungi, parasites, DNA and RNA.

11. The microfluidic chip for analyte detection according to any one of claim 10, characterized in that: The virus is at least one of influenza A virus, influenza B virus, respiratory syncytial virus and SARS-CoV-2 virus.

12. The microfluidic chip for analyte detection according to any one of claims 1 to 8, characterized in that: The reaction chamber is filled with at least one reagent for detecting an analyte.

13. The microfluidic chip for analyte detection according to claim 12, characterized in that: The reagent is in a freeze-dried state.

14. The microfluidic chip for analyte detection according to claim 12, characterized in that: One of the reagents is a chromogenic agent.

15. The microfluidic chip for analyte detection according to claim 12, characterized in that: One of the reagents is a fluorescent signal detection agent.

16. A microfluidic chip for analyte detection, characterized in that: It includes a chip body, a pressure cover body and an exhaust liquid blocking film; The chip body is provided with a flow distribution chamber, a reaction chamber and a sample adding chamber; The sample adding chamber is connected to the reaction chamber through a sample adding channel, and the reaction chamber is connected to the waste liquid hole through an overflow channel; The exhaust liquid blocking film covers the exhaust end of the waste liquid hole; The pressure cover body has a rubber plug; The sum of the volumes of the sample addition channel, the liquid separation channel, the exhaust channel, the exhaust hole and the flow diversion cavity is greater than the volume of the rubber plug; When the rubber plug is inserted into the sample adding cavity, the solution flows from the sample adding cavity into the reaction cavity, and the excess solution flows from the reaction cavity into the waste liquid hole.

17. The microfluidic chip for analyte detection according to claim 16, characterized in that: The pressure cover body is threadedly connected to the sample adding chamber.

18. The microfluidic chip for analyte detection according to claim 16, characterized in that: In the thickness direction of the chip body, the chip body has a first side and a second side; The overflow channel is opened on the first side, and the sample addition channel is opened on the second side.

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