Composition for enhancing skin elasticity or alleviating skin wrinkles

Abstract

The present disclosure relates to a composition for enhancing skin elasticity or alleviating skin wrinkles. The composition for enhancing skin elasticity or alleviating skin wrinkles of the present disclosure exhibits a remarkable synergistic effect on enhancing skin elasticity or alleviating skin wrinkles by means of a combination of adenosine triphosphate and cyanocobalamin.

Description

Composition for improving skin elasticity or wrinkles

[0001] This application claims priority to Republic of Korea Application No. 10-2023-0162485, filed November 21, 2023, Republic of Korea Application No. 10-2023-0162486, filed November 21, 2023, and Republic of Korea Application No. 10-2024-0120276, filed September 4, 2024, all of which are hereby incorporated by reference in their entirety.

[0002] The present disclosure relates to a composition for improving skin elasticity or skin wrinkles.

[0003] With the aging population and rising life expectancy, interest in anti-aging skin care, which prioritizes improving skin elasticity and wrinkle reduction, is growing. Skin, comprising approximately 16% of the human body's volume, is the outermost organ, protecting us from the external environment and irritants. Compared to internal organs, skin is constantly exposed to external irritants, making it vulnerable to attack. Facial skin, in particular, is directly exposed to UV rays and pollutants, making it more susceptible to aging than other parts of the body.

[0004] Skin aging can be divided into intrinsic aging, which occurs naturally, and extrinsic aging, which is triggered by external factors. Genetic factors are cited as the primary factor in intrinsic aging, which determines the structure and changes in skin cells and tissues. Meanwhile, extrinsic aging is caused by external factors, such as nutritional deficiencies, UV rays, and environmental pollution. Unlike genetic factors, these factors are preventable and avoidable.

[0005] These factors cause skin aging, leading to dryness and roughness, wrinkles, and loss of elasticity. It is known that skin wrinkles and loss of elasticity are directly related to the decrease and deformation of collagen and elastin, the main proteins in the dermis. In other words, skin wrinkles begin with dermal aging, and the structure of elastin, an elastic fiber, changes, causing the skin to sag and wrinkle. Furthermore, numerous studies have reported that increased activity of matrix metalloproteinases (MMPs), which break down connective tissue components including collagen in the dermis, inhibits collagen synthesis, thereby increasing wrinkles.

[0006] MMPs are calcium-dependent, zinc-containing metalloproteinases with an active center that are secreted in vivo as latent proenzymes (zymogens). To achieve enzymatic activity, MMPs undergo structural modification, cleaving the amino-terminal region and activating it. Activated MMPs are regulated by inhibitors such as 2-macroglobulin and TIMP (tissue inhibitors of metalloproteinase), leading to the secretion of MMPs by a vast majority of cells, including keratinocytes and fibroblasts. These MMPs are divided into various subfamilies based on their structural and functional properties. Among them, MMP1 is an interstitial collagenase, a protein essential for wound healing, tissue regeneration, and reorganization. Furthermore, MMP3 (Stromelysin-1) is a protein enzyme that degrades collagen, elastin, and fibronectin. When exposed to exogenous or endogenous stimuli, these MMP1 or MMP3 are produced in larger amounts than usual in the body, bind to collagen, and then cut the collagen, causing skin wrinkles and loss of elasticity.

[0007] There is a need to discover new effective ingredients that can have a beneficial effect on improving skin elasticity and wrinkles by suppressing factors that cause decreased skin elasticity or wrinkles as described above.

[0008] Therefore, the problem that the present disclosure seeks to solve is to provide a composition that has a remarkably excellent effect of improving skin elasticity or improving skin wrinkles.

[0009] In order to solve the above problem, the inventors of the present disclosure conducted extensive research and found that adenosine triphosphate (ATP) and cyanocobalamin (vitamin B) 12 ) was found to significantly increase the effect of improving skin elasticity or improving skin wrinkles. The inventors of the present disclosure surprisingly discovered that when adenosine triphosphate and cyanocobalamin were combined, the expression levels of collagen-decomposing enzymes MMP1 and MMP3 were synergistically reduced, thereby confirming that the effect of improving skin elasticity or improving skin wrinkles was significantly excellent, thereby completing the present invention.

[0010] The present disclosure provides a composition for improving skin elasticity or wrinkles, comprising adenosine triphosphate and cyanocobalamin. The composition may include adenosine triphosphate and cyanocobalamin as active ingredients.

[0011] In one aspect of the present disclosure, the adenosine triphosphate may be included in an amount of 0.0001 to 20 wt%, preferably 0.001 to 10 wt%, based on the total weight of the composition. If the adenosine triphosphate is less than 0.0001 wt% based on the total weight of the composition, the effect of improving skin elasticity or wrinkles may be weak, and if it exceeds 20 wt%, the stability of the formulation may be impaired.

[0012] In one aspect of the present disclosure, the cyanocobalamin may be included in an amount of 0.00001 to 1 wt%, preferably 0.00005 to 0.01 wt%, based on the total weight of the composition. If the cyanocobalamin is included in an amount of less than 0.00001 wt% based on the total weight of the composition, the effect of improving skin elasticity or wrinkles may be weak, and if it is included in an amount of more than 1 wt%, the stability of the formulation may be impaired.

[0013] In one aspect of the present disclosure, the weight ratio of the adenosine triphosphate and cyanocobalamin may be 1: 0.001 to 10, preferably 1: 0.05 to 5, more preferably 1: 0.01 to 1, even more preferably 1: 0.02 to 0.5, and most preferably 1: 0.03 to 0.1 (adenosine triphosphate: cyanocobalamin). When the two components are included in the weight ratio range, the effect of improving skin elasticity or improving skin wrinkles may be significantly increased.

[0014] In one aspect of the present disclosure, the enhancement of skin elasticity may refer to enhancing skin elasticity by increasing the amount of collagen and elastin in dermal tissue. Collagen, a collagenous fibrous protein that accounts for 90% of the dermis, can provide mechanical strength to skin, connective tissue resistance, and tissue cohesion. Elastin, a protein with exceptional elasticity, forms a meshwork structure with collagen, thereby imparting elasticity to the skin.

[0015] In one aspect of the present disclosure, the improvement of skin wrinkles may mean preventing or improving wrinkles of the skin caused by aging or external factors by increasing the amount of collagen and elastin in the dermis.

[0016] In one aspect of the present disclosure, the composition can exhibit effects of enhancing skin elasticity or improving skin wrinkles by inhibiting the expression of MMP1 and MMP3 in the dermis. Specifically, the composition can increase the amount of collagen in the dermis by inhibiting MMPs, which degrade collagen, thereby enhancing the cohesiveness and firmness of skin tissue, thereby enhancing skin elasticity and improving wrinkles.

[0017] In one aspect of the present disclosure, the composition for improving skin elasticity or improving skin wrinkles, including adenosine triphosphate and cyanocobalamin, may be a cosmetic composition. The cosmetic composition may further include conventional adjuvants and carriers, such as conventionally used antioxidants, stabilizers, solubilizers, vitamins, pigments, or fragrances. For example, the cosmetic composition may further include auxiliary ingredients such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, or allantoin.

[0018] In one aspect of the present disclosure, since the cosmetic composition is essentially applied to the skin, it can be manufactured in any formulation commonly manufactured with reference to cosmetic compositions in the art. For example, it can be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, oil, powder foundation, emulsion foundation, wax foundation, or spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible toner, a nourishing toner, a nourishing cream, a massage cream, an essence, an eye cream, a spray, or a powder.

[0019] In one aspect of the present disclosure, when the formulation of the cosmetic composition is a paste, cream or gel, the carrier component may include animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide.

[0020] In one aspect of the present disclosure, when the cosmetic composition is in the form of a powder or spray, the carrier component may include lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder, and in particular, when it is a spray, the carrier component may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0021] In one aspect of the present disclosure, when the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizer, or an emulsifier may be included as a carrier component, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan may be included.

[0022] In one aspect of the present disclosure, when the formulation of the cosmetic composition is a suspension, the carrier component may include a liquid diluent such as water, ethanol, or propylene glycol; a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester; microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tragacanth.

[0023] In one aspect of the present disclosure, the composition for improving skin elasticity or wrinkles, including adenosine triphosphate and cyanocobalamin, may be a health functional food composition. The health functional food may include food additives acceptable from a food science perspective, and may further include suitable carriers, excipients, or diluents commonly used in the manufacture of health functional foods.

[0024] In one aspect of the present disclosure, the health functional food composition may be used as a food additive, and in this case, the composition may be added as is or used together with other foods or food ingredients according to a conventional method. The amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment). Examples of the types of the health functional food include dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes. In addition to the above, the health functional food composition may include various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, or carbonating agents used in carbonated beverages.

[0025] Additionally, the present disclosure provides a method for improving skin elasticity or wrinkles, comprising applying a composition for improving skin elasticity or wrinkles, comprising adenosine triphosphate and cyanocobalamin, to the skin. The same technical features as those of the composition for improving skin elasticity or wrinkles can be applied to the method.

[0026] All ingredients described in the present disclosure preferably do not exceed the maximum usage levels stipulated in relevant laws and regulations of Korea, China, the United States, Europe, Japan, etc. (e.g., Regulations on Cosmetic Safety Standards (Korea), Cosmetic Safety Technical Standards (China), Food Code (Korea), Food Additives Code (Korea), Health Functional Food Code (Korea)). That is, preferably, the cosmetic or health functional food composition according to the present disclosure includes the ingredients according to the present invention within the content limits permitted by relevant laws and regulations of each country.

[0027] The composition for improving skin elasticity or wrinkles of the present disclosure has a remarkable synergistic effect on improving skin elasticity or wrinkles through the combination of adenosine triphosphate and cyanocobalamin.

[0028] Figure 1 is a graph comparing the expression levels of MMP1 or MMP3 when adenosine triphosphate and cyanocobalamin were treated alone or in combination in Experimental Example 1.

[0029] Figure 2 is a graph comparing the reduction rate of MMP1 or MMP3 expression when adenosine triphosphate and cyanocobalamin were treated alone or in combination in Experimental Example 1.

[0030] Figure 3 is a graph comparing the MMP1 expression reduction rate according to the weight ratio of adenosine triphosphate and cyanocobalamin (adenosine triphosphate: cyanocobalamin in that order) in Experimental Example 2.

[0031] Hereinafter, the present invention will be described in detail, using examples and the like, to aid understanding. However, the examples according to the present invention may be modified in various different forms, and the scope of the present invention should not be construed as being limited to the following examples. The examples of the present invention are provided to more fully explain the present invention to those of average skill in the art.

[0032]

[0033] Adenosine triphosphate (disodium adenosine triphosphate) used in the following experimental examples was purchased from the manufacturer Jiangsu Huayu chemical, China (Hanju CNC), and cyanocobalamin was purchased from the manufacturer DSM, China (Serochem).

[0034]

[0035] Experimental Example 1: Experiment on the synergistic effect of adenosine triphosphate (ATP) and cyanocobalamin on improving skin elasticity and wrinkles.

[0036] The combined use of adenosine triphosphate (ATP) and cyanocobalamin in skin cells was evaluated for their effects on improving skin elasticity and wrinkle improvement. Matrix metalloproteinases (MMPs) are factors that influence skin elasticity and wrinkles, and in this experimental example, the synergistic effect of regulating the expression of two types of MMPs, MMP1 and MMP3, was evaluated. MMPs are matrix proteins that degrade collagen, and more than 20 types are known. Degrading collagen reduces skin elasticity and leads to wrinkles. Therefore, inhibiting MMP expression can improve skin elasticity and improve wrinkles.

[0037] Human foreskin-derived human fibroblasts (Hs68; human fibroblast, CRL-1635) were purchased from ATCC. The purchased cells were cultured in a medium containing 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin in DMEM (Dulbecco's Modified Eagle Medium-GIBCO) at 37°C in a 5% CO2 incubator.

[0038] To analyze the efficacy of changes in MMP1 and MMP3 mRNA expression, 2*10 human fibroblasts were seeded in a 6-well plate. 5After dispensing cells / well, they were cultured in a 37°C, 5% CO2 incubator for one day. After replacing the medium with PBS, they were exposed to UVA (365 nm) 10 J / cm using an RMX 3W (Vilber Lourmat) device. 2 was investigated. After adding 10 ppm of ATP and 1 ppm of cyanocobalamin to the medium, the cells were cultured for one day under the same conditions. Afterwards, total RNA was obtained using the AccuPrep Universal RNA Extraction Kit (Bioneer, K-3140), and then AccuPower® RocketScript TM cDNA was synthesized using Cycle (Bioneer, K-2202). The expression levels of each MMP1 and MMP3 gene were analyzed by qPCR using ABI7500 Real Time PCR using the synthesized cDNA and taqman probes (assay ID: MMP1 (Hs00899658), MMP3 (Hs00968305)).

[0039] The synergistic effect was determined using the Colby equation. A synergistic effect was determined when the combined effect measurement (T) exceeded the Colby equation prediction (E). The Colby equation is as follows:

[0040] E = (X+Y)-(XХY / 100)

[0041] X: Efficacy compared to control group when cyanocobalamin was used alone

[0042] Y: Efficacy compared to the control group measured when ATP was used alone

[0043] E: Predicted efficacy compared to the control group when using a combination of cyanocobalamin and ATP

[0044] T: Efficacy compared to the control group when using a combination of cyanocobalamin and ATP

[0045]

[0046] As shown in the experimental results in Figures 1 and 2, UVA irradiation increases collagen-degrading enzymes MMP1 and MMP3, but when adenosine triphosphate (ATP) or cyanocobalamin is treated alone, MMP1 and MMP3 are confirmed to decrease slightly. In particular, when adenosine triphosphate (ATP) and cyanocobalamin are treated in combination, the MMP1 expression reduction rate and MMP3 expression reduction rate are significantly improved compared to the individual treatment groups, indicating that the combination of the two components produces a synergistic effect.

[0047]

[0048] Experimental Example 2: Experiment on the synergistic effect of a combination of adenosine triphosphate (ATP) and cyanocobalamin on improving skin elasticity and wrinkles according to the weight ratio.

[0049] Human foreskin-derived human fibroblasts (Hs68; human fibroblast, CRL-1635) were purchased from ATCC. The purchased cells were cultured in a medium containing 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin in DMEM (Dulbecco's Modified Eagle Medium-GIBCO) at 37°C in a 5% CO2 incubator.

[0050] To analyze the efficacy of changes in MMP1 mRNA expression, 2*10 human fibroblasts were seeded in a 6-well plate. 5 After dispensing cells / well, they were cultured in a 37°C, 5% CO2 incubator for one day. After replacing the medium with PBS, they were exposed to UVA (365 nm) 10 J / cm using an RMX 3W (Vilber Lourmat) device. 2The weight ratios of ATP and cyanocobalamin were changed to 10 ppm: 10 ppm, 10 ppm: 1 ppm, 10 ppm: 0.3 ppm, or 10 ppm: 0.1 ppm (in the order of ATP: cyanocobalamin), and then added to the medium, and cultured for one day under the same conditions.

[0051] Afterwards, total RNA was obtained using AccuPrep Universal RNA Extraction Kit (Bioneer, K-3140) and then AccuPower® RocketScript TM cDNA was synthesized using Cycle (Bioneer, K-2202). The expression levels of each MMP1 gene were analyzed by qPCR using ABI7500 Real Time PCR using the synthesized cDNA and taqman probe (assay ID: MMP1 (Hs00899658)).

[0052] Looking at the experimental results shown in Fig. 3, it was confirmed that a synergistic effect occurred when the weight ratio of ATP and cyanocobalamin was 10 ppm:1 ppm and 10 ppm:0.3 ppm, exceeding the Colby predicted value. When the weight ratio was 10 ppm:0.1 ppm, the Colby predicted value was exceeded, but there was no significant difference when combined compared to cyanocobalamin 0.1 ppm alone.

Claims

1. A composition for improving skin elasticity or wrinkles, comprising adenosine triphosphate and cyanocobalamin.

2. A composition for improving skin elasticity or skin wrinkles, wherein the adenosine triphosphate is contained in an amount of 0.0001 to 20 wt% based on the total weight of the composition in paragraph 1.

3. A composition for improving skin elasticity or skin wrinkles, wherein the cyanocobalamin is contained in an amount of 0.00001 to 1 wt% based on the total weight of the composition in paragraph 1.

4. A composition for improving skin elasticity or skin wrinkles in the first paragraph, wherein the weight ratio of adenosine triphosphate and cyanocobalamin is 1: 0.001 to 10 (adenosine triphosphate: cyanocobalamin).

5. In the first paragraph, the composition is a composition for improving skin elasticity or skin wrinkles by inhibiting the expression of MMP1 and MMP3.

6. In the first paragraph, the composition is a cosmetic composition for improving skin elasticity or improving skin wrinkles.

7. In the first paragraph, the composition is a composition for improving skin elasticity or improving skin wrinkles, which is a health functional food composition.

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