Gelled feed composition for dibranchiate, method for producing same, and aquaculture method for dibranchiate

The gelatinized feed composition for bivalves, containing taurine and other amino acids along with protein, addresses the challenges of water quality deterioration and growth inefficiency in traditional bivalve culturing methods, achieving enhanced feeding efficiency and growth rates.

WO2025135073A1PCT designated stage expired Publication Date: 2025-06-26NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY
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Patent Information

Application Number
PCT/JP2024/044794
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-12-18
Filing Date
2024-12-18
Publication Date
2025-06-26

AI Technical Summary

Technical Problem

Existing methods for culturing bivalves such as octopus and squid face challenges including water quality deterioration and insufficient growth, making them impractical for widespread use.

Method used

A gelatinized feed composition for bivalves containing compounds like taurine, glycine, alanine, and arginine, along with protein derived from various sources, is used to efficiently culture bivalves. The feed is produced by cutting a protein-containing material, adding the specified compounds and salt, and gelling the mixture.

Benefits of technology

The gelatinized feed composition significantly improves feeding efficiency and growth rates of bivalves, such as octopus and squid, compared to traditional feeding methods, thereby providing a viable culturing method.

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Abstract

The purpose of the present invention is to provide an actually usable aquaculture method for octopus or the like. The above-mentioned problem can be solved by a gelled feed composition for dibranchiates according to the present invention, the feed composition containing: at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine; and a protein.
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Description

Gelled feed composition for bibranchs, method for producing the same, and method for cultivating bibranchs

[0001] TECHNICAL FIELD The present invention relates to a gelled feed composition for dibranchs, a method for producing the same, and a method for cultivating dibranchs. TECHNICAL FIELD According to the present invention, dibranchs can be cultivated efficiently.

[0002] Attempts have been made to cultivate bibranchs such as octopuses and squids (Patent Documents 1 and 2). For example, Patent Document 1 discloses a cultivation method in which octopuses raised in a cultivation module are fed with live or frozen crustaceans or mollusks, or semi-moist or dry feed. Patent Document 2 discloses a cultivation method in which octopuses are fed with feed contained in a casing.

[0003] JP 10-262488 A JP 2023-109148 A

[0004] However, this method has been difficult to use as a farming method due to the deterioration of water quality during feeding and the insufficient growth of the octopus. Therefore, an object of the present invention is to provide a farming method for octopus and the like that can be actually used.

[0005] The present inventors have conducted extensive research into methods for cultivating octopuses and other animals, and have surprisingly found that bibranchial fish such as octopus or squid can be efficiently cultivated using a gelled feed composition for bibranchial fish containing a compound such as taurine and a protein. The present invention is based on this finding. Therefore, the present invention provides: [1] a gelled feed composition for bibranchs, comprising at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a protein; [2] a gelled feed composition for bibranchs according to [1], wherein the protein is derived from seafood, livestock, poultry, wild birds, insects, or vegetable proteins; [3] a method for cultivating bibranchs, comprising a step of feeding bibranchs gelled feed comprising at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a protein; [4] a method for cultivating bibranchs according to [3], wherein the protein is derived from seafood, livestock, poultry, wild birds, insects, or vegetable proteins; [5] A method for producing a gelled feed for bibranchs, comprising: (1) a step of cutting a protein-containing material; (2) a step of adding at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a salt to the cut material to obtain a mixture; and (3) a step of gelling the mixture. [6] The method for producing a gelled feed for bibranchs according to [5], wherein the protein-containing material is derived from seafood, livestock meat, poultry, wild bird meat, insects, or vegetable protein.

[0006] According to the gelled feed composition for bibranchia of the present invention, bibranchia such as octopus or squid can be efficiently cultivated.

[0007] Photograph of octopus cultivated by the method of the present invention. Photograph of a gelled feed composition for bibranchia containing taurine (0.2% by weight or 2% by weight) using Alaska pollock. Graph showing feeding rates when a gelled feed composition for bibranchia containing Alaska pollock and taurine (0.2% by weight or 2% by weight) was fed to octopus for six days. Graph showing feeding rates when a gelled feed composition for bibranchia containing horse mackerel and taurine (0.2% by weight or 2% by weight) was fed to octopus for six days. Graph showing feeding rates when a gelled feed composition for bibranchia containing Alaska pollock and glycine (0.1% by weight) was fed to octopus for six days. Graph showing feeding rates when a gelled feed composition for bibranchia containing Alaska pollock and alanine (0.1% by weight) was fed to octopus for six days. 1 is a graph showing the feeding rate of a gelled feed composition for bibranchs containing Alaska pollock and arginine (0.5% by weight) fed to octopus for 6 days.

[0008] [1] Gelled feed composition for dibranchia The gelled feed composition for dibranchia of the present invention contains at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a protein.

[0009] <<Taurine, Glycine, Alanine, and Arginine>> The gelled feed composition for dibranchia of the present invention contains taurine, glycine, alanine, or arginine, with taurine being particularly preferred. The content of these components is not particularly limited as long as the effects of the present invention are obtained, but the upper limit relative to the composition is, for example, 30% by weight or less, preferably 10% by weight or less, more preferably 5% by weight or less, and even more preferably 3% by weight or less. The lower limit is, for example, 0.001% by weight or more, preferably 0.01% by weight or more, more preferably 0.05% by weight or more, and even more preferably 0.1% by weight or more. The upper and lower limits can be arbitrarily combined. The inclusion of these components improves the intake of the feed composition by dibranchia, allowing dibranchia to grow efficiently.

[0010] <<Protein>> The gelled feed composition for bibranchia of the present invention contains protein. Examples of proteins include, but are not limited to, proteins derived from seafood, livestock meat, poultry, wild birds, insects, or vegetable proteins, with seafood being preferred. More specifically, examples include gelatin, soy protein, rice protein, wheat protein, corn protein, chicken, pork, and beef. The origin of the seafood is also not particularly limited as long as the effects of the present invention are achieved. Examples include Alaska pollock, sardine, mackerel, sea bream, flounder, plaice, herring, crab, shark, Atka mackerel, short-necked clam, scallop, clam, oyster, corbicula, rainbow trout, salmon, horse mackerel, threadfin tuna, sedge croaker, hairtail, catfish, squid, shrimp, and octopus. The protein content is not particularly limited as long as the effects of the present invention are obtained. The upper limit of the protein content relative to the composition is, for example, 70% by weight or less, preferably 45% by weight or less, more preferably 40% by weight or less, and even more preferably 35% by weight or less. The lower limit is, for example, 1% by weight or more, preferably 7% by weight or more, more preferably 10% by weight or more, and even more preferably 15% by weight or more. The upper and lower limits can be arbitrarily combined. The protein preferably contains polyunsaturated fatty acids such as DHA and EPA. Polyunsaturated fatty acids may be added to the protein. The polyunsaturated fatty acid content is not limited, but is, for example, 0.1 to 30% by weight, preferably 1 to 10% by weight, relative to the total weight of the gelled feed composition for bibranchs.

[0011] <<Salt>> The gelled feed composition for bibranchia of the present invention contains a salt. By including a salt, proteins can be gelled. The salt is not particularly limited as long as it can gel, but examples thereof include sodium chloride, potassium chloride, calcium chloride, and magnesium chloride. The salt content is not particularly limited as long as gelling occurs, but is, for example, 0.5 to 10 wt %, preferably 1 to 5 wt %, and more preferably 2 to 4 wt %.

[0012] The gelled feed composition for bibranchia of the present invention contains water. The amount of water is not particularly limited as long as the gelled feed composition is gelled, but is, for example, 30 to 99 wt %, in one embodiment 55 to 92 wt %, and in another embodiment 60 to 90 wt %.

[0013] Gelling promoter: The gelling feed composition for bibranchia of the present invention may contain a gelling promoter. In particular, gelling promoters may be used when the feed is derived from poultry, wild game, insect, or vegetable proteins. Examples of gelling promoters include gelatin, calcium chloride, transglutaminase, sodium alginate, starch, carrageenan, soy protein, and egg white.

[0014] Dibranchia are octopuses or squids. Examples of octopuses include the common octopus, Japanese octopus, Pacific octopus, long-legged octopus, willow octopus, ringed octopus, striped octopus, and long-legged octopus. Examples of squid include neon flying squid, cuttlefish, spear squid, Japanese common squid, Pacific flying squid, Japanese giant squid, lorigo squid, Ilex, Japanese common squid, Japanese sand squid, Japanese swordtip squid, firefly squid, Japanese giant squid, diamondback squid, lightning squid, two-spotted squid, bigfin reef squid, Japanese giant ...

[0015] <Hardness of Gelled Composition> The gelled feed composition for dibranchia of the present invention is a gelled composition. The hardness of the gelled feed composition is not particularly limited, as long as it is edible by dibranchia and does not easily crumble in water. For example, when measured using a spherical plunger with a diameter of 5 mm at an analysis speed of 1 mm / sec, the upper limit of the breaking strength is 50 N or less, preferably 20 N or less, more preferably 10 N or less, and even more preferably 5 N or less. The lower limit is, for example, 0.01 N or more, preferably 0.1 N or more, more preferably 0.5 N or more, and even more preferably 1 N or more. The upper and lower limits can be combined in any manner. By having the hardness within the above range, the gelled feed does not crumble in water and does not pollute the water. Furthermore, dibranchia can embrace and ingest the gelled feed.

[0016] [2] Method for producing a gelled feed composition for dibranchia The method for producing a gelled feed composition for dibranchia of the present invention comprises: (1) a step of cutting a protein-containing material; (2) a step of adding at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a salt to the cut material to obtain a mixture; and (3) a step of gelling the mixture.

[0017] <<Protein Material Cutting Step (1)>> In the protein material cutting step (1), the protein material is cut. As the protein material, any of the protein-containing materials described in the above section "[1] Gelled Feed Composition for Bibranchs" can be used without any restrictions. The cut protein material may be surimi or minced meat, either of which can be gelled. For example, in the case of surimi, any of the production methods commonly used in this field can be used without any restrictions. Examples include a method of manually extracting meat or a method using a meat extractor. In the case of minced meat, any of the production methods commonly used in this field can be used without any restrictions.

[0018] <<Mixing Step (2)>> In the mixing step (2), at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a salt are added to the cleaved protein material to obtain a mixture. The compound may be any of the taurine, glycine, alanine, and arginine compounds described in the above section "[1] Gelled Feed Composition for Dibranchia," with taurine being preferred. The amount of compound added is not particularly limited as long as gelation occurs. The upper limit is, for example, 30% by weight or less, preferably 10% by weight or less, more preferably 5% by weight or less, and even more preferably 3% by weight or less, relative to the resulting mixture. The lower limit is, for example, 0.001% by weight or more, preferably 0.01% by weight or more, more preferably 0.05% by weight or more, and even more preferably 0.1% by weight or more. The upper and lower limits can be arbitrarily combined. The salt may be any of the salts described in the above section "[1] Gelled Feed Composition for Dibranchia," without any restrictions. The amount of salt added is not particularly limited, but is, for example, 0.5 to 10% by weight, preferably 1 to 5% by weight, and more preferably 2 to 4% by weight, based on the resulting mixture.

[0019] <Gelation Step (3)> In the gelation step (3), the mixture is gelled. A conventional method used in this field can be used for gelation. Heating may be used for gelation, but gelation can also occur at low temperatures. The heating temperature for gelation is not particularly limited, but a higher temperature can shorten the gelation time. That is, heating can shorten the processing time. The upper limit of the gelation temperature is 300°C or lower, preferably 99°C or lower, and more preferably 95°C or lower. The lower limit is 0°C or higher, preferably 5°C or higher, more preferably 10°C or higher, even more preferably 20°C or higher, and even more preferably 30°C or higher. The gelation time is also not limited as long as gelation is complete, but is 10 minutes to 48 hours, preferably 30 minutes to 24 hours.

[0020] The heating method is not particularly limited as long as gelation is promoted, and for example, methods such as steaming, boiling, baking, and frying can be used. Steaming can be performed by boiling water to generate steam. The steaming temperature is not particularly limited, and the upper limit is 150°C or less in one embodiment, 130°C or less in another embodiment, 110°C or less in another embodiment, 100°C or less in another embodiment, and 95°C or less in another embodiment. The lower limit is 1°C or more in one embodiment, 60°C or more in one embodiment, 65°C or more in another embodiment, and 70°C or more in another embodiment. The upper and lower limits can be appropriately combined to create a suitable range.

[0021] As a boiling method, the mixture can be boiled in heated water. The boiling temperature is not particularly limited, but the upper limit is 105°C or less in one embodiment, 100°C or less in one embodiment, 95°C or less in one embodiment, 90°C or less in one embodiment, and 85°C or less in another embodiment. The lower limit is 50°C or more in one embodiment, 60°C or more in one embodiment, 65°C or more in one embodiment, and 70°C or more in another embodiment. The upper and lower limits can be appropriately combined to set a suitable range.

[0022] As a grilling method, the mixture can be grilled over a charcoal fire or a gas flame. The grilling temperature is not particularly limited, but for example, the surface temperature of a charcoal fire is 300 to 600°C, and the surface temperature of a gas flame is approximately 200 to 250°C. The mixture is grilled at a certain distance from these heat sources. The surface temperature of the mixture is not limited, but in order to prevent the mixture from burning, the upper limit is 180°C or less in some embodiments, 160°C or less in some embodiments, 150°C or less in some embodiments, and 140°C or less in some embodiments. The lower limit is 90°C or more in some embodiments, 100°C or more in some embodiments, 110°C or more in some embodiments, 120°C or more in some embodiments, and 130°C or more in some embodiments. The upper and lower limits can be appropriately combined to create a suitable range.

[0023] As a frying method, the mixture can be fried in heated oil. The frying temperature is not particularly limited, but the upper limit is 220°C or less in one embodiment, 200°C or less in one embodiment, 190°C or less in one embodiment, and 180°C or less in one embodiment. The lower limit is 130°C or more in one embodiment, 140°C or more in one embodiment, 150°C or more in one embodiment, 160°C or more in one embodiment, and 170°C or more in one embodiment. The upper and lower limits can be appropriately combined to create a suitable range.

[0024] Alternatively, gelation can be achieved by keeping the mixture at a low temperature in a refrigerator, etc. The upper and lower limits can be appropriately combined to set a suitable range.

[0025] (Gelling Accelerator) In the production method of the present invention, a gelling accelerator can also be used. Examples of gelling accelerators include gelatin, calcium chloride, transglutaminase, sodium alginate, starch, soy protein, and egg white. The amount of gelling accelerator added may be 0.0001 to 50% by weight based on the mixture, and a person skilled in the art can adjust the amount added as appropriate.

[0026] [3] Method for cultivating bibranchs The method for cultivating bibranchs of the present invention includes a step of feeding bibranchs a gelled feed containing at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a protein. The gelled feed for bibranchs used in the method for cultivating bibranchs of the present invention can be the gelled feed composition for bibranchs described in the above section "[1] Gelled feed composition for bibranchs." Furthermore, the gelled feed composition for bibranchs can be obtained by the manufacturing method described in the above section "[2] Method for producing a gelled feed composition for bibranchs."

[0027] The dibranchia cultivated by the method of the present invention are octopuses or squids. Examples include the common octopus, Japanese octopus, Pacific octopus, long-legged octopus, willow octopus, ringed octopus, striped octopus, and long-legged octopus. Examples of squid include neon flying squid, cuttlefish, spear squid, Japanese common octopus, Pacific common octopus, Japanese giant squid, rorigan, Ilex, Japanese common squid, Japanese sand squid, swordtip squid, firefly squid, Japanese giant squid, colossal squid, diamondback squid, thunder squid, two-spotted squid, bigfin reef squid, Japanese giant ...

[0028] Dibranchs feed by embracing their food. They have taste-sensing sensory organs in their feet, and use these to judge the taste of the food they are embracing. Therefore, feed that is large enough to be easily embraced is preferred for aquaculture. The shape of the gelled feed composition for dibranchs is not particularly limited, as long as it can be embraced by a dibranch, but examples include square, rectangular, cylindrical, triangular prism, rectangular prism, sphere, and ellipse. The size of the gelled feed composition for dibranchs is also not particularly limited, as long as it can be embraced by a dibranch. For example, for a common octopus weighing approximately 200 g, a feed weight of approximately 10 g to 60 g is preferred, and for a common octopus weighing approximately 400 g, a feed weight of approximately 40 g to 120 g is preferred.

[0029] The water temperature during cultivation can be set appropriately depending on the type of dibranch, but can be, for example, 0 to 35° C. For example, in the case of common octopus, the temperature is 10 to 30° C., preferably 15 to 28° C.

[0030] The present invention will be specifically described below with reference to examples, but these examples are not intended to limit the scope of the present invention.

[0031] Example 1 In this example, a gelled feed composition for bibranchs was prepared using Alaska pollock and taurine. Sodium chloride (3 wt%) and taurine (0.2 wt%), or sodium chloride (3 wt%) and taurine (2 wt%), were added to Alaska pollock surimi (100 g) and then crushed. The resulting mixture was heated and maintained at 80-90°C for 30 minutes to gel. Figure 2 shows a photograph of the resulting gelled feed composition. The gelled feed composition was cut into square pieces (approximately 20 g) that the octopus could hold, and fed to the octopus for six days. Frozen squid was also fed as a comparative example. As shown in Figure 3, a higher feeding rate was observed compared to frozen squid, which is typically used as octopus feed.

[0032] Example 2 In this example, a gelled feed composition for bibranchs was prepared using horse mackerel and taurine. The procedure of Example 1 was repeated, except that horse mackerel was used instead of Alaska pollock, to obtain a gelled feed composition, which was then fed to common octopus. As shown in Figure 4, the feeding rate was higher than that of frozen squid.

[0033] Example 3 In this example, a gelled feed composition for bibranchs was prepared using Alaska pollock and glycine (0.1% by weight). The procedure of Example 1 was repeated, except that glycine was used instead of taurine, to obtain a gelled feed composition, which was then fed to common octopus. As shown in Figure 5, the feeding rate was higher than that of frozen squid.

[0034] Example 4 In this example, a gelled feed composition for bibranchs was prepared using Alaska pollock and alanine (0.1% by weight). The procedure of Example 1 was repeated, except that alanine was used instead of taurine, to obtain a gelled feed composition, which was then fed to common octopus. As shown in Figure 6, the feeding rate was higher than that of frozen squid.

[0035] Example 5 In this example, a gelled feed composition for bibranchs was prepared using Alaska pollock and arginine (0.5% by weight). The procedure of Example 1 was repeated, except that arginine was used instead of taurine, to obtain a gelled feed composition, which was then fed to common octopus. As shown in Figure 7, the feeding rate was higher than that of frozen squid.

[0036] The gelled feed composition of the present invention can be effectively used for cultivating bibranchs such as octopus or squid.

Claims

1. A gelled feed composition for dibranchia, comprising at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a protein.

2. The gelled feed composition for dibranchs according to claim 1, wherein the protein is derived from seafood, livestock meat, poultry, wild game, insects, or vegetable proteins.

3. A method for cultivating dibranchs, comprising a step of feeding dibranchs a gelled feed containing at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a protein.

4. The method of cultivating dibranchs as described in claim 3, wherein the protein is derived from seafood, livestock meat, poultry, wild game, insects, or vegetable proteins.

5. A method for producing a gelled feed for dibranchs, comprising: (1) a step of cutting a material containing protein; (2) a step of adding at least one compound selected from the group consisting of taurine, glycine, alanine, and arginine, and a salt to the cut material to obtain a mixture; and (3) a step of gelling the mixture.

6. The method for producing gelled feed for dibranchs according to claim 5, wherein the protein-containing material is derived from seafood, livestock meat, poultry, wild game, insects, or vegetable proteins.

Citation Information

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