A functional dietary supplement containing hazelnut extracts enriched with hazelnut anticancer components

Abstract

The invention relates to a functional dietary supplement and contains at least one of the group of the kernel, shell, or cell powder of hazelnuts having anticancer, nutritive, and flavoring properties as active ingredients, and hazelnut anticancer components.

Description

[0001] A FUNCTIONAL DIETARY SUPPLEMENT CONTAINING HAZELNUT EXTRACTS ENRICHED WITH HAZELNUT ANTICANCER COMPONENTS

[0002] TECHNICAL FIELD

[0003] The invention relates to a functional dietary supplement and contains at least one of the group of the kernel, shell, or cell powder of hazelnuts having anticancer, nutritive, and flavoring properties as active ingredients, and hazelnut anticancer components.

[0004] PRIOR ART

[0005] Dietary supplements are products that are generally obtained from plant sources and contain various components in pure or mixture form. People use dietary supplements to be protected against and treat various chronic diseases, especially cancer, to help maintain and improve general health, or to take anti-aging measures.

[0006] Hazelnut contains a very high amount of tocopherols and tocotrienols (vitamin E). It also contains various flavonoids and phenolic acids (ferulic acid, caffeic acid, etc.). With these compounds they contain, hazelnuts will be able to serve as a dietary component with antioxidant properties for users.

[0007] Hazelnut, as is known in the art, is a food component with high nutritional value. It contains about 600 to 700 calories of energy, protein at a value of 14 to 20 grams, carbohydrates at a value of 50 to 60 grams, and fiber at a value of 8 to 20 grams. Also, hazelnuts contain B vitamins and minerals. In this aspect, hazelnuts have high nutritional value for users owing to these nutritional values they contain.

[0008] Since hazelnut is a food component that fights free radicals with the compounds it contains, it has a structure that can reduce cellular damage and prevent oxidative stress. With this property, hazelnuts can be used as an anti-aging food component for users.

[0009] Owing to such properties, research and development activities are carried out to effectively include hazelnuts as a food component in functional dietary supplements. SUMMARY OF THE INVENTION

[0010] The use of hazelnuts as food components in food products / supplements is known in the art. Patent application no. TR2016 / 03611 describes a food product obtained by processing hazelnut kernel, while patent application no. TR2018 / 07184 describes the use of hazelnut leaves as tea. Also, there are studies in the technical field on the preparation of extracts of various components from hazelnut kernels and shells.

[0011] There are various food products / supplements derived from certain parts of hazelnuts, but the production of new dietary supplements for the relevant technical field is necessary for the advancement of the relevant technical field.

[0012] The inventors of the present invention aim to introduce a dietary supplement for the relevant technical field that effectively contains hazelnut anticancer components. For this, a functional dietary supplement enriched with anticancer components within hazelnuts is obtained.

[0013] The inventors of the present invention aim to introduce a functional dietary supplement containing hazelnuts with high nutritional values. For this, it introduces a functional dietary supplement containing enriched hazelnut extracts.

[0014] The inventors of the present invention aim to introduce a functional dietary supplement containing nuts with high aroma and flavor.

[0015] DETAILED DESCRIPTION OF THE INVENTION

[0016] In this detailed description, the subject of the invention relates to a functional dietary supplement, and is described only with the examples that will not create any limiting effect for a better understanding of the subject.

[0017] The dietary supplement of the invention contains the relevant parts of hazelnut as the main active ingredient. The inventors of the present invention aim to obtain the beneficial chemical compounds and nutritional values of hazelnuts in the most effective way and to include them in dietary supplements. In this invention, the dietary supplement can be contained in capsules or tablets, in powder form, in liquid form, in chocolate or bars, in granules. The scope of protection of the invention is not limited to the final product form of the dietary supplement. However, the most preferred product form for the subject of the invention is the tablet or capsule form. Accordingly, the dietary supplement obtained in the invention has a configuration to be placed in capsules or tablets.

[0018] The components in the dietary supplement of the invention can be in powder or liquid form. However, this is optional, and with the methods known in the art, those in powder form can be converted into liquid, and liquid components into powder. The scope of protection of the invention is not limited to the solid or liquid form of the components in the dietary supplement.

[0019] The dietary supplement of the invention has a hazelnut-containing composition. Accordingly, the dietary supplement contains hazelnut kernel (i.e. fruit) as a component. Hazelnut kernels are primarily used to give aroma and flavor to the dietary supplement. In addition, hazelnut kernels, as is known in the art, have very high nutritional value and compounds that provide antioxidant properties.

[0020] The nutritional content of hazelnuts essentially includes fatty acids, proteins, vitamins, fibers, and amino acids. Fatty acids constitute 66-72% of the weight of hazelnuts, with the majority (92% by weight in fatty acids) unsaturated. Unsaturated fatty acids of hazelnut include oleic acid (78-84%), linoleic acid (10-18%), and linolenic acid (0.15- 0.37%) and it has a structure rich in oleic acid. After crops, shelled fruits have the highest fiber content, and hazelnuts are in second place among these fruits. Hazelnut nutritional fibers are known to be effective in maintaining general health and preventing cardiovascular diseases, colon cancer, and type 2 diabetes.

[0021] In this invention, in a preferred embodiment, hazelnut kernels are included in a solid, that is, ground form, within the dietary supplement. In a preferred embodiment, hazelnuts are ground with steel blade grinders until they reach the targeted sizes.

[0022] The dietary supplement of the invention contains the shell of hazelnuts as a component. Hazelnuts, as is known, have hard brown or green shells. The presence of paclitaxel and its derivatives (10-deacetyl baccatin III, baccatin III, cephalomannine) has been detected in said hazelnut shells (regardless of color). In the technique, paclitaxel, an alkaloid from the diterpene class, has a mechanism of action and broad-spectrum anti-neoplastic properties. It blocks mitosis by supporting the polymerization of a and p-tubulin heterodimers that form microtubules in the G2 phase during the cell cycle.

[0023] It is possible to grind hazelnut shells for use. Firstly, cleaning processes must be carried out because the microorganisms contained in it must be removed. Accordingly, cleaning processes are preferably carried out primarily with water and then with ozonated water. In another preferred embodiment, shells can be purified from microorganisms by means of ultrasonic bathing. In the most preferred embodiment, each of these processes is applied.

[0024] Preferably, drying processes are carried out on the hazelnut shells subjected to cleaning processes. Because it is preferred that hazelnut shells have a brittle structure in order to be ground effectively. For this, a temperature value between 60 to 75 °C is preferred, especially where the active compounds in the hazelnut shell / leaves are not damaged. Afterthis process, it is ensured that the hazelnut shells subjected to grinding process are ground to specified targets. The dietary supplement of this invention contains hazelnut shells that can pass through sieves with pore values of 2 mm or less as a component. The reason for these values is that it is aimed to obtain a homogeneous dietary supplement.

[0025] The dietary supplement of the invention contains hazelnut leaf as a component. Hazelnut leaf, similar to hazelnut shell, contains paclitaxel and its derivatives. It is also a part rich in terms of antioxidant content. Adding hazelnut leaves as a component to the dietary supplement will allow the functionality of the final product to increase.

[0026] Hazelnut leaves, similar to hazelnut shells, are first collected, then cleaned, and then ground. The reason for applying production process steps in this way is to ensure that it is cleaned of microorganisms and that it remains homogeneously in the final product in the targeted sizes. Cleaning processes are carried out with water and / or ozonated water. Subsequently, it is subjected to drying processes for grinding processes. The reason for this is that it is aimed to have a brittle structure for grinding processes. For this, a value between 60 to 75 °C is preferred, especially where the active compounds in the hazelnut shell / leaves are not damaged. After this process, it is ensured that the hazelnut leaves subjected to grinding process are ground to specified targets. The dietary supplement of this invention contains hazelnut leaves that can pass through sieves with pore values of 2 mm or less as a component. The reason for these values is that it is aimed to obtain a homogeneous dietary supplement.

[0027] The dietary supplement of the invention contains hazelnut cell powder as a component. In this invention, hazelnut cell powder means the final components obtained as a result of suspension culturing processes.

[0028] Hazelnut cell suspension samples obtained after culturing processes are collected. The obtained hazelnut cell suspension cultures are taken into tubes in a preferred embodiment. Centrifugation processes are applied to the tubes containing cultures. This centrifugation process is preferably carried out at a value in the range of 7500 to 10000 rpm for a period of 10 to 20 minutes. Centrifugation processes are carried out to obtain the precipitate to be processed from the solution. The precipitate obtained after this process is turned into powder so that it can be included in the dietary supplement.

[0029] The powderization process is carried out by lyophilization process in a preferred embodiment. The lyophilization process is carried out for at least 24 hours, preferably at a temperature in the range of -50 to -60 °C.

[0030] Hazelnut cell powder contains paclitaxel and its derivatives. It is also a part rich in terms of antioxidant content. Adding hazelnut cell powder as a component to the dietary supplement will allow the functionality of the final product to increase.

[0031] The dietary supplement of the invention contains the extract containing hazelnut anticancer components. Hazelnut cell powder is used as a raw material to obtain extracts containing hazelnut anticancer components. The hazelnut cell powder obtained as characterized by the previous process step of the invention is subjected to washing processes with dichloromethane in a preferred embodiment. Instead of the dichloromethane mentioned here, other compounds that can perform the same function can also be used for washing processes.

[0032] In a preferred embodiment, incubation processes are performed in an ultrasonication device on the hazelnut cell powders subjected to washing processes. Other devices that provide the same function can also be used for incubation processes. Here, the incubation process is preferably 1 hour. The supernatant obtained as a result of the incubation process is collected and transferred to the tubes for the next process steps.

[0033] A mixture is obtained by adding methanol:water solution to the tubes containing the supernatant. Here, methanol-water is a solution containing methanol at a value between 80% and 90% by weight in a preferred embodiment. Then, n-hexane is added to this mixture in a preferred embodiment. The n-hexane:methanol-water ratio in this mixture is a value in the range of 8:1 to 4:1. The obtained mixture is subjected to centrifugation processes to obtain new supernatant liquid to be used in the next processes. This centrifugation process is preferably carried out at a value in the range of 7500 to 10000 rpm for a period of 10 to 20 minutes. The supernatant liquid obtained is evaluated as an extract and subjected to washing processes with dichloromethane. In a preferred embodiment, the extract obtained is subjected to removal processes, preferably at 40 °C under vacuum in order to remove the methanol it may contain.

[0034] The dietary supplement of the invention contains extract containing hazelnut anticancer components as a component. In dietary supplements, the extract is in liquid form, as can be expected. The dietary supplement contains an extract containing hazelnut anticancer components at a value in the range of 1 % to 10% (v / w) of the extract. While determining the ratio of hazelnut anticancer compounds in the product, the amounts known to be effective on cancer cells in scientific studies were taken into account. Concentrations below this amount may reduce the effectiveness of the compounds, while concentrations above it are likely to cause side effects.

[0035] The dietary supplement of the invention contains the hazelnut extract containing anticancer components, as well as hazelnut cell powder as a component. Hazelnut cell powder obtained from hazelnut cell cultures is at a value in the range of 1 % to 10% (w / w) in the dietary supplement. While determining the ratio of hazelnut cell powder obtained from hazelnut cell cultures in the product, the amounts known to be effective on cancer cells in scientific studies were taken into account. In the studies conducted, using the concentrations of obtaining hazelnut anticancer compounds, the value of the amount to reach bioactive properties was calculated. Concentrations below this amount may reduce the effectiveness of the compounds, while concentrations above it are likely to cause side effects. The dietary supplement of the invention contains the extract containing hazelnut anticancer components and hazelnut cell powder as a component, in addition, it contains at least one of the group of powdered hazelnut shells, hazelnut kernels, and hazelnut leaf as a component.

[0036] In a preferred embodiment, the dietary supplement contains ground hazelnut kernels, hazelnut shells and hazelnut leaf components all together. The total presence of these components is at a value in the range of 80% to 98% (w / w).

[0037] The dietary supplement of the invention contains hazelnut shells in the range of 20% to 50% (w / w), hazelnut kernels in the range of 20% to 50% (w / w), and hazelnut leaves in the range of 10% to 30% (w / w). The different hazelnut parts in the product and their ratios have been determined by taking into account that the hazelnut product to be produced contains sufficient amounts of compounds for its physical structure, consistency, taste, and bioactivity. It has been determined that the ratio of phenolic compounds obtained from the shell, which constitutes 42% of the total biomass of hazelnuts by weight, varies between 0.5-1 .5% by weight. For this reason, in order to have the envisaged ratio of bioactive content in the dietary supplement, it must be present in the product in the range of 20% to 50% (w / w). Also, the shell being a byproduct allows the utilization of useful compounds that are not used. In addition, the physical structure of the hazelnut shell contributes to the formation of the physical integrity of the dietary supplement composition. Therefore, it is used in high ratios in the product. Hazelnut kernel is the most commercially valuable part and is known to contain protein, fatty acids, vitamins and phenolic compounds in abundance. Further, it is the part that gives the hazelnut its unique taste. In order for the dietary supplement to have the envisaged biological activity, hazelnut kernels are used at a value in the range of 20% to 50% (w / w). Further, it must be present in certain ratios to ensure the taste of the product. It is known that there is a higher ratio of bioactive compounds (phenolics) in hazelnut leaves than in hazelnut kernels. However, due to their seasonal formation, the product contains hazelnut leaves at a value in the range of 10% to 30% (w / w). Thus, the enriched content envisaged in the product is ensured.

[0038] Functional dietary supplements / supplements to be obtained with the hazelnut shell / hazelnut kernel / leaf components and hazelnut cell powder / FAB to be added can be added to many foods, and in addition to the nutritional properties of foods, their qualities in terms of preventive health, especially for cancer, can be increased. The product / products obtained can be used in the future to increase the nutritional and functionality values of hazelnut-based vegetable milks from plant dairy products.

[0039] The scope of protection of the invention is specified in the appended claims and cannot be limited to what is described for illustrative purposes in this detailed description. It is clear that a person skilled in the art can produce similar embodiments in the light of what is explained above, without deviating from the main theme of the invention.

Claims

CLAIMS1. A functional dietary supplement suitable for use to provide nutritional value, to protect against-treat chronic diseases, to prevent-delay aging, characterized in that it contains as a component hazelnut cell powders obtained from hazelnut cell suspension cultures, and extract obtained from hazelnut cell powders containing hazelnut anticancer components.

2. A dietary supplement according to claim 1 , characterized in that it contains hazelnut cell powders at a value in the range of 1 % to 10% by weight.

3. A dietary supplement according to one of the preceding claims, characterized in that it contains the extract containing hazelnut anticancer components at a value in the range of 1 % to 10% (v / w).

4. A dietary supplement according to one of the preceding claims, characterized in that it contains at least one of the group of hazelnut kernels, hazelnut shells and / or hazelnut leaves as a component.

5. A dietary supplement according to claim 4, characterized in that it comprises hazelnut kernels at a value in the range of 20% to 50% by weight.

6. A dietary supplement according to claim 4 or 5, characterized in that it comprises hazelnut shells at a value in the range of 20% to 50% by weight.

7. A dietary supplement according to one of claim 4-6, characterized in that it comprises hazelnut leaves at a value in the range of 10% to 30% by weight.

8. A dietary supplement according to one of the preceding claims, characterized in that it comprises hazelnut shells at a value in the range of 20% to 50% (w / w), hazelnut kernels at a value in the range of 20% to 50% (w / w), hazelnut leaves at a value in the range of 10% to 30% (w / w), hazelnut cell powders at a value in therange of 1% to 10% by weight, and extract obtained from hazelnut cell powders at a value in the range of 1% to 10%.