Preparation method for probiotic sanitary product

By mixing inactivated probiotic powder with hydrophilic oil agent to form a bacterial suspension and coating fibers to produce probiotic sanitary products, the problem of uneven distribution of probiotics is solved, the antibacterial effect and production efficiency are improved, and the cost and risks are reduced.

WO2025153082A1PCT designated stage expired Publication Date: 2025-07-24CHONGQING BAIYA SANITARY PRODUCTS CO LTD
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Patent Information

Application Number
PCT/CN2025/073122
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-01-19
Filing Date
2025-01-17
Publication Date
2025-07-24

AI Technical Summary

Technical Problem

The distribution of probiotic sanitary napkins in the prior art is uneven, resulting in the antibacterial rate not meeting the standard requirements, and the preparation process of live bacteria is high and the safety is low.

Method used

Inactivated probiotic powder is mixed with hydrophilic oil agent, and then the bacterial suspension is formed and fiber is coated. The fiber is used to produce probiotic sanitary products to ensure uniform distribution and strong adhesion of probiotics.

Benefits of technology

It achieves the uniform distribution of probiotics on sanitary products, improves the antibacterial effect, complies with the antibacterial performance standards of textiles, reduces production costs and safety risks, and is suitable for efficient industrial production.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the field of sanitary product production. Disclosed is a preparation method for a probiotic sanitary product. The preparation method comprises the following preparation steps: step A, adding ultrapure water; step B, adding an oil agent and stirring, comprising the following steps: B1: metering the oil agent and then adding the oil agent into ultrapure water to form a mixed solution, the oil agent being a hydrophilic oil agent; and B2: stirring the mixed solution; step C, cooling the mixed solution; step D, adding a probiotic powder to form a probiotic suspension, comprising the following steps: D1: metering the probiotic powder and then adding the probiotic powder into the mixed solution at a time to form the probiotic suspension, the probiotic powder being a probiotic powder (non-viable type) in which an oil-soluble protective agent is added; and D2: stirring the probiotic suspension; step E, coating fibers with the probiotic suspension; and step F, utilizing the fibers to produce the probiotic sanitary product. The scheme can solve the technical problem that, in the prior art, living probiotics are adopted for preparing sanitary products, and the probiotics on the sanitary products are distributed unevenly.
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Description

Preparation method of probiotic sanitary products Technical Field

[0001] The present invention relates to the field of sanitary product production, and in particular to a method for preparing a probiotic sanitary product. Background Art

[0002] Probiotics are active microorganisms. The lactobacilli they contain are the most abundant bacteria in the normal female vagina. In a healthy vaginal environment, the abundance of lactobacilli can reach 97.56% to 99.99%. They can produce substances such as lactic acid, hydrogen peroxide, and some enzymes, which are important for maintaining the stability of the vaginal microecological environment. Therefore, it is generally believed that the addition of probiotics can enhance the efficacy of sanitary napkins, maintain the balance of vaginal flora, and maintain a healthy female reproductive environment. Probiotic sanitary napkins are an upgrade to traditional sanitary napkin fabrics. The probiotic-containing surface layer of the sanitary napkin is hot air non-woven fabric, which belongs to the category of hot air bonding (hot rolling, hot air) non-woven fabrics. Hot air non-wovens are made by combing ordinary ES fibers, then using hot air from a drying device to penetrate the fiber web, causing it to be heated and bonded.

[0003] The prior art discloses a method for preparing a sanitary napkin containing vaginal probiotics (application publication number: CN106267319A). The method comprises two steps: preparing a freeze-dried powder of the vaginal probiotics and evenly distributing the freeze-dried powder on the absorbent layer of the sanitary napkin. The preparation of the freeze-dried powder requires the use of standardized gynecological examinations and vaginal secretion collection methods. Samples must be collected within 3-7 days after the end of menstruation before the freeze-dried powder is applied. This cumbersome preparation process is not suitable for industrial production requiring high production efficiency.

[0004] The prior art also discloses a feminine hygiene product containing probiotics and a preparation method thereof (application publication number: CN 102120045A). Water-soluble probiotic freeze-dried powder is dissolved in sterile water to obtain a bacterial suspension with a total viable bacterial count of 1×109 to 1012 cfu / ml. The bacterial suspension is then directly sprinkled on a sanitary napkin and dried at low temperature (less than 45°C) to obtain the finished product.

[0005] The existing technology has the following technical problems:

[0006] 1. Although the existing technology can be applied to industrial production, there are currently strict control indicators for the antibacterial rate of sanitary napkin products. The inventors used this solution to conduct experiments and found that the sanitary napkins produced did not meet the requirements for the antibacterial rate of textiles in the current GB / T20944.3-2008 standard. The fundamental reason is that during the process of reproduction and metabolism, live bacteria are prone to accumulation due to activity, resulting in uneven distribution of probiotics on the sanitary napkin, causing the antibacterial rate to fail to meet the standard requirements.

[0007] 2. Freeze-dried powder is live bacteria. Live bacteria are directly sprinkled on sanitary napkins. In order to ensure the activity of live bacteria, the production conditions are more stringent. Not only is the cost high, but it may also produce harmful substances, resulting in reduced safety of use. Summary of the Invention

[0008] The present invention aims to provide a method for preparing probiotic sanitary products, so as to solve the technical problem of uneven distribution of probiotics on sanitary products in the prior art of using live bacteria to prepare sanitary products.

[0009] In order to achieve the above object, the present invention adopts the following technical solutions:

[0010] The preparation method of probiotic sanitary products comprises the following preparation steps:

[0011] Step A, adding ultrapure water;

[0012] Step B, adding oil and stirring, comprises the following steps:

[0013] B1: Add the oil agent to ultrapure water after measurement to form a mixed solution, wherein the oil agent is a hydrophilic oil agent;

[0014] B2: stirring the mixed solution;

[0015] Step C, cooling the mixed solution;

[0016] Step D, adding probiotic powder to form a bacterial suspension, comprises the following steps:

[0017] D1: Adding a measured amount of probiotic powder to the mixed solution at once to form a bacterial suspension. The probiotic powder is a probiotic powder (non-viable bacteria type) with an oil-soluble protective agent added thereto.

[0018] D2: stirring the bacterial suspension;

[0019] Step E, coating the fiber with the bacterial suspension;

[0020] Step F: producing probiotic sanitary products using the fiber.

[0021] Invention concept and principle:

[0022] In order to solve the technical problem that the use of live bacteria in the prior art to prepare sanitary napkins easily leads to uneven distribution of probiotics, the inventors replaced them with a bacterial suspension containing inactivated probiotics. The inactivated probiotics retain the original characteristics of the live bacteria but no longer have the ability to continue to grow and reproduce. After testing the prepared sanitary products, the inventors found that the improvement in the uniformity of the probiotic distribution was limited. At the same time, it was also found that the prior art solution using water-soluble freeze-dried probiotic powder easily formed defects on the surface of the sanitary products. After several experiments, the inventors finally creatively obtained a technical solution including the steps of preparing probiotic powder (non-live bacteria type) with the addition of an oil-soluble protective agent and coating the fiber with the bacterial suspension and then using the fiber to produce probiotic sanitary products, which effectively solved the problems of uneven distribution of probiotics and defects.

[0023] The principles and advantages of this solution are:

[0024] 1. Compared with the solution in the prior art that uses sterile water to dissolve water-soluble probiotic freeze-dried powder, a probiotic powder (non-viable type) with an oil-soluble protective agent is added to a mixed solution formed by ultrapure water mixed with an oil agent to form a bacterial suspension containing inactivated probiotics. Inactivated probiotics are a type of postbiotics and are more stable and safer than the live bacteria disclosed in the prior art. After being diluted and dissolved in the mixed solution, they have obvious antibacterial effects on Escherichia coli, Staphylococcus aureus, Candida albicans, etc., and meet the requirements for antibacterial properties of textiles in the GB / T20944.3-2008 standard.

[0025] 2. The purpose of mixing the hydrophilic oil agent in ultrapure water is that the fiber itself is not hydrophilic or directly water-repellent, and only after the hydrophilic oil agent is added can it have the ability to absorb water. Therefore, in the preparation method of special products such as sanitary napkins and tampons that need to have water-absorbing functions, the step of adding a hydrophilic oil agent is basically included; the inventors found that the oil agent itself is a highly viscous substance, which is conducive to making the inactivated probiotics on sanitary products difficult to peel off, and also has an impact on improving the antibacterial effect of sanitary products. The probiotic powder with added oil-soluble protective agent is oil-soluble powder and can be well dissolved with the oil agent. On the contrary, the water-soluble probiotic freeze-dried powder used in the prior art has poor solubility with the oil agent. If the bacterial suspension prepared with the water-soluble probiotic freeze-dried powder is used to coat the fiber, the water-soluble probiotic freeze-dried powder and the oil agent on the fiber surface are easily accumulated into clumps, forming dots attached to the probiotic sanitary products. These dots are what are often called surface defects in textile fabrics.

[0026] 3. The existing technology is to directly apply or spray the bacterial suspension on the non-woven fabric used for sanitary napkins, while this solution is to coat the fiber with the bacterial suspension, and then use the fiber to produce probiotic sanitary products. Not only is the probiotic distribution on the prepared sanitary products very uniform, but the probiotics on the sanitary products also have strong adhesion and are not easy to lose, so that the quality of the formed sanitary products is guaranteed and the antibacterial stability is better.

[0027] 4. Experiments have shown that the probiotic sanitary products prepared using this preparation method are not only effective in antibacterial effects, but also promote the growth of beneficial bacteria including Lactobacillus gasseri. Lactobacillus gasseri can regulate the acid-base balance in the body, improve the healthy environment, and enhance immunity, thereby effectively inhibiting the growth and reproduction of harmful bacteria and maintaining the vaginal environment. However, it is also easy to reduce or even disappear. By using probiotic sanitary products prepared using this preparation method, the problem of repeated gynecological inflammation in women can be fundamentally solved.

[0028] 5. All the steps included in this solution can be automatically operated by machines, and the raw materials used, such as oils and probiotic powders, are conventional industrial materials. Compared with the preparation methods in the prior art that include the use of standardized gynecological examinations and vaginal secretion collection methods, the preparation process is less complicated and is more suitable for industrial production environments with high production efficiency requirements.

[0029] Preferably, as an improvement, the step of adding ultrapure water comprises:

[0030] A1: Ultrapure water is measured and added to the mixing tank;

[0031] A2: Heat the mixing tank to raise the temperature of the ultrapure water to 60-80°C. Stir the ultrapure water continuously during the heating process.

[0032] Beneficial effects: Different types of oils require different water temperatures when preparing mixed solutions. When using hydrophilic oils to prepare mixed solutions, ultrapure water needs to be heated to 60-80°C in advance. Too high or too low a water temperature will affect the emulsification process when adding probiotic powder to the mixed solution after preparation. The purpose of "continuous stirring" during this heating period is to ensure uniform water temperature throughout the mixing tank.

[0033] Preferably, as an improvement, in the step of adding oil and stirring, the mass of oil in A1 is measured to be 0.35%-0.45% of the mass of the fiber to be coated, and in the step of adding probiotic powder, the mass of probiotic powder in A1 is measured to be 0.5%-2.5% of the mass of the fiber to be coated.

[0034] Beneficial effects: As a hydrophilic auxiliary agent, the oil agent content in the fiber is too low or too high, which will have an adverse effect on the formed sanitary products. If the fiber oil content is too low, the antistatic property of the sanitary products will deteriorate, and the adhesion of inactivated probiotics will be weakened, thereby affecting its antibacterial effect; if the fiber oil content is too high, the adhesion of the fiber will increase, the friction resistance will increase, and the uniformity of the fiber web will be affected. As a skin-friendly material, the sanitary products will give customers a poor experience. Experiments have shown that when the oil agent mass is measured at 0.35%-0.45% of the mass of the fiber to be coated, and the probiotic powder is measured at 0.5%-2.5% of the mass of the fiber to be coated, the antibacterial effect and the experience of the formed sanitary products can be better balanced.

[0035] Preferably, as an improvement, in the step of cooling the mixed solution, the mixed solution needs to be cooled to below 50°C.

[0036] Beneficial effects: If the mixed solution is not cooled, the efficacy of the inactivated probiotics will be reduced. It is worth mentioning that inactivated probiotics are not the same as dead bacteria. Dead bacteria have no efficacy, while inactivated probiotics retain the original characteristics of live bacteria, but do not have the ability to grow and reproduce.

[0037] Preferably, as an improvement, the oil-soluble protective agent comprises, by mass, 10% linolenic acid, 15% trehalose, 5% glycerol, 6% lauryl alcohol, 2% L-arginine, 1% conjugated linolenic acid, and the balance is water.

[0038] Beneficial effects: In the oil-soluble protective agent containing the above ingredients, unsaturated fatty acids and sugars serve as effective protective substances, forming a flexible protective layer outside the cells. They can be dissolved in oils of different sources in any proportion between 0-20% (w / w). After dissolution, the probiotic powder is in an unstable state without any stratification, precipitation or agglomeration, effectively avoiding the formation of defects on sanitary products by the powder, and having a positive effect on the surface quality and antibacterial rate of the molded sanitary products.

[0039] Preferably, as an improvement, in the step of stirring the bacterial suspension, the stirring speed is 6000-12000 rpm / min, and the stirring time is 60-70 min.

[0040] Beneficial effect: The purpose of stirring the bacterial suspension is to ensure the emulsification stability within the bacterial suspension. A stirring speed that is too low will result in uneven stirring, while a stirring speed that is too high will easily generate bubbles, resulting in an unstable bacterial suspension, which in turn affects the antibacterial properties of the formed sanitary products.

[0041] Preferably, as an improvement, in the step of coating the fiber with the bacterial suspension, the bacterial suspension needs to be continuously stirred at a stirring speed of 40-50 rpm / min, and the bacterial suspension is simultaneously kept warm at a temperature of 40-45°C.

[0042] Beneficial effect: The purpose of continuously stirring the bacterial suspension in the step of coating the fiber with the bacterial suspension is to ensure the uniformity of the inactivated probiotics in the bacterial suspension. The stirring speed is greatly reduced compared with that before coating. This is because the bacterial suspension is already sufficiently uniform before coating, and it only needs to be stirred at a lower speed to maintain the uniformity. If it is still set to a high speed, it will be a waste of energy.

[0043] Preferably, as an improvement, in the step of stirring the bacterial suspension, the stirring speed is 10000 rpm / min and the stirring time is 65 min.

[0044] Beneficial effect: When the stirring speed is 10,000 rpm / min and the stirring time is 65 minutes, the bacterial suspension can be fully emulsified, the stability of the inactivated probiotics on the molded sanitary products is greatly improved, and the antibacterial rate is increased the most.

[0045] Preferably, as an improvement, the probiotic sanitary product is a hot air non-woven fabric.

[0046] Beneficial effects: The production process of hot air non-woven fabrics is an advanced production process with the advantages of high efficiency, high quality and low cost.

[0047] Preferably, as an improvement, the surface layer, the guide layer and the covering layer of the guide core are made of hot air non-woven fabric.

[0048] Beneficial effects: The hot air non-woven fabric made by the hot air penetration bonding process has the characteristics of being fluffy and soft, and has high openness, breathability, ductility and strength. At the same time, the hot air non-woven fabric produced by the above preparation method has a higher antibacterial rate against Escherichia coli, Staphylococcus aureus, Candida albicans and other bacteria than ordinary non-woven fabrics. The surface layer, guide layer, and covering layer of the guide core of women's sanitary napkins that can contact the skin and are easily stained with menstrual blood require these characteristics. [0048.1][Corrected 11.02.2025 according to Rule 26] FIG1 is a graph showing the test results of the probiotic non-woven fabric and blank non-woven fabric prepared in Example 1 for promoting the growth of Lactobacillus gasseri. DETAILED DESCRIPTION

[0049] Example 1

[0050] A method for preparing a hot air nonwoven fabric is as follows:

[0051] Step A: Add ultrapure water

[0052] A1: Add ultrapure water into the mixing tank after measurement;

[0053] A2: Heat the mixing tank to raise the temperature of the ultrapure water to 70°C to ensure the emulsification effect of the mixing; the ultrapure water needs to be stirred continuously during the heating process to ensure the uniformity of the water temperature in the entire mixing tank.

[0054] Step B: Add oil and stir

[0055] B1: 4 kg of oil was weighed and added to the mixing tank to form a mixed solution of ultrapure water and oil. The oil used in this example is a hydrophilic oil (also called a "hydrophilic agent"), which is an anionic surfactant containing phosphate, sulfonate, and succinate components. It can adsorb on the surface of solid particles and improve the interaction between particles, and has good hydrophilicity.

[0056] B2: Stir the mixed solution of ultrapure water and oil at a stirring speed of 100 rpm / min for ≥30 min.

[0057] Step C: Cooling the mixed solution

[0058] C1: Cool the mixed solution to 50°C.

[0059] Step D: Add probiotic powder to form bacterial suspension

[0060] D1: 12.5 kg of probiotic powder was measured and added to a mixing tank at once to form a bacterial suspension. In this embodiment, the probiotic powder used was a probiotic powder (non-viable bacteria type) to which an oil-soluble protective agent was added. The oil-soluble protective agent included 10% linolenic acid, 15% trehalose, 5% glycerol, 6% lauryl alcohol, 2% L-arginine, and 1% conjugated linolenic acid, with the remainder being water (by mass fraction).

[0061] D2: Stir the bacterial suspension at a speed of 10,000 rpm / min for 65 min;

[0062] Step E: Coating the fiber with bacterial suspension

[0063] E1: Pour the bacterial suspension in the preparation tank into the storage tank used for coating;

[0064] E2: Fiber coating. In this example, the fiber mass to be coated is 1000 kg. During the fiber coating process, the bacterial suspension needs to be continuously stirred at a stirring speed of 45 rpm / min. At the same time, the bacterial suspension is kept warm at a temperature of 43°C.

[0065] Step F: Using fibers to produce hot air nonwoven fabrics

[0066] F1: The fibers are fed into a hot air oven for thermal bonding to form a hot air nonwoven fabric (hereinafter referred to as "probiotic nonwoven fabric").

[0067] In embodiments other than this embodiment, a female sanitary napkin is also disclosed, including a surface layer, a guide layer, and a guide core, wherein any one or more layers of the surface layer, the guide layer, and the guide core are made of the hot air non-woven fabric produced above.

[0068] Example 2

[0069] The difference between this embodiment and the first embodiment is that, in the step of adding probiotic powder, the amount of probiotic powder in A1 is 0.5% of the mass of the fiber to be coated, that is, 5 kg.

[0070] Example 3

[0071] The difference between this embodiment and the first embodiment is that, in the step of adding probiotic powder, the amount of probiotic powder in A1 is 2.5% of the mass of the fiber to be coated, that is, 25 kg.

[0072] Example 4

[0073] The difference between this embodiment and the first embodiment is that, in the step of adding probiotic powder, the probiotic powder is measured in A1 to be 1% of the mass of the fiber to be coated, that is, 10 kg.

[0074] Example 5

[0075] The difference between this embodiment and the first embodiment is that, in the step of adding probiotic powder, the amount of probiotic powder in A1 is 2% of the mass of the fiber to be coated, that is, 20 kg.

[0076] Comparative Example 1

[0077] The difference between this comparative example and Example 1 is that in step E, the bacterial suspension prepared in step D is directly sprayed or coated onto the ready-made hot air non-woven fabric to form a probiotic non-woven fabric with inactivated probiotics.

[0078] Comparative Example 2

[0079] The difference between this comparative example and Example 1 is that the probiotic powder added in step 4 is water-soluble probiotic powder (non-viable bacteria type) without adding an oil-soluble protective agent.

[0080] Experimental example

[0081] The probiotic non-woven fabric prepared in Example 1 was subjected to an antibacterial test using the test method specified in GB / T 20944.3-2008 Evaluation of antibacterial properties of textiles Part 3: Oscillation method.

[0082] The test results are as follows:

[0083]

[0084] According to the evaluation standard GB / T20944.3-2008, a sample with an inhibition rate of ≥70% against Staphylococcus aureus and Escherichia coli is considered to have an antibacterial effect, and a sample with an inhibition rate of ≥60% against Candida albicans is considered to have an antibacterial effect.

[0085] The results in Table 1 show that in terms of antibacterial properties, the antibacterial rate of Example 1 against Escherichia coli (8099) reached 72%, showing an antibacterial effect; the antibacterial rate of Example 1 against Staphylococcus aureus (ATCC6538) reached 80%, showing an antibacterial effect; and the antibacterial rate of Example 1 against Candida albicans (ATCC10231) reached 63%, showing an antibacterial effect.

[0086] The antibacterial properties of the probiotic non-woven fabrics prepared in Example 2, Example 3, Example 4, Example 5, Comparative Example 1, and Comparative Example 2 were tested using the test method GB / T 20944.3-2008 Evaluation of antibacterial properties of textiles Part 3: Oscillation method.

[0087] The test results for E. coli 8099 are as follows:

[0088] Example 2 Example 3 Example 4 Example 5 Comparative Example Comparative Example 2 Antibacterial rate (%) 70 71 73 71 52 62 Evaluation Antibacterial effect Antibacterial effect Antibacterial effect Antibacterial effect No antibacterial effect No antibacterial effect Table 2

[0089] The test results for Staphylococcus aureus ATCC6538 are as follows:

[0090] Example 2 Example 3 Example 4 Example 5 Comparative Example Comparative Example 2 Antibacterial rate (%) 717778705965 Evaluation Antibacterial effect Antibacterial effect Antibacterial effect Antibacterial effect No antibacterial effect No antibacterial effect Table 3

[0091] The test results for Candida albicans ATCC10231 are as follows:

[0092]

[0093] From the test results in Tables 2, 3, and 4, it can be seen that the antibacterial rates of the probiotic non-woven fabric in Comparative Example 1 against Escherichia coli, Staphylococcus aureus, and Candida albicans are 52%, 59%, and 43%, respectively, and the evaluation is that they have no antibacterial effect. After in-depth research, the fundamental reason lies in that in step E, the bacterial suspension prepared in step D is directly sprayed or applied to the ready-made hot air non-woven fabric. This step cannot ensure the uniformity of the distribution of probiotics on the non-woven fabric, thereby adversely affecting the antibacterial rate of the prepared probiotic non-woven fabric.

[0094] The surfaces of the probiotic non-woven fabrics prepared in Example 1, Example 2, Example 3, Example 4, Example 5, and Comparative Example 2 were observed with the naked eye. It was found that the surfaces of the probiotic non-woven fabrics in Examples 1 to 5 were relatively smooth and no obvious defects were produced, while the surface of the probiotic non-woven fabric in Comparative Example 2 had obvious defects, which not only affected the antibacterial rate of the probiotic non-woven fabric, but also affected the antibacterial rate of the probiotic non-woven fabric. As shown in Tables 2 to 4, the antibacterial rates of the probiotic non-woven fabric in Comparative Example 2 against Escherichia coli, Staphylococcus aureus, and Candida albicans were 62%, 65%, and 54%, respectively. The evaluation showed that they had no antibacterial effect, and the poor skin-friendliness during use was predictable, which easily led to a bad user experience.

[0095] The probiotic non-woven fabric prepared in Example 1 and a blank non-woven fabric (common non-woven fabric without probiotics added on the market) were tested for their efficacy in promoting the growth of Lactobacillus gasseri. The test method was the plate count method, and the operating steps were as follows:

[0096] 1. Sample pretreatment

[0097] Weigh the probiotic non-woven fabric and blank non-woven fabric and cut them into 1cm 2 The large and small fragments were placed in 100 mL of liquid MRA culture medium and sterilized for later use.

[0098] 2. Reagent Preparation

[0099] Liquid MRA medium: glucose 2%, lactose 1%, peptone 1%, yeast extract 0.5%, beef extract powder 1%, dipotassium hydrogen phosphate 0.2%, Tween-80 0.1%, magnesium sulfate 0.058%, manganese sulfate 0.019%, L-cysteine ​​0.1%;

[0100] Solid culture medium: Dissolve 66.2g of finished MRA culture medium in 1000mL of pure water and add 0.1% L-cysteine. 3. Activation of bacteria

[0101] Transfer 800 μL of Lactobacillus gasseri from the glycerol tube to a test tube containing 9 mL of MRA liquid medium and incubate at 37°C for 24 hours. Inoculate the above bacterial suspension at a 2% (v / v) inoculum into MRA liquid medium and incubate at 37°C for 16 hours. Reserve the bacterial suspension for later use.

[0102] 4. Co-cultivation of bacteria

[0103] The Lactobacillus gasseri suspension was inoculated at a 2% (v / v) inoculum volume into liquid MRA culture medium containing probiotic non-woven fabrics and blank non-woven fabrics, respectively. The blank control was liquid MRA culture medium containing 2% Lactobacillus gasseri suspension and cultured at 37°C for 16 hours.

[0104] 5. Plate count

[0105] Perform a 10-fold serial dilution, selecting three appropriate dilutions. Spread 100 μL of each co-culture solution evenly onto solid culture medium and incubate anaerobically at 37°C for 48 hours. After incubation, observe the growth of colonies on the three plates at different dilutions and count the colonies.

[0106] The test results are as follows:

[0107] According to the results shown in Figure 1, the bacterial concentration in the Lactobacillus gasseri suspension was 1.53×10 8 CFU / mL, the bacterial concentration in the co-culture solution of Lactobacillus gasseri with the addition of blank non-woven fabric reached 1.77×10 8 CFU / mL, the bacterial concentration in the co-culture solution of Lactobacillus gasseri with the addition of probiotic non-woven fabric reached 1.90×10 9 CFU / mL, which shows that the probiotic non-woven fabric has a certain growth-promoting effect on Lactobacillus gasseri.

[0108] The above are only embodiments of the present invention, and common knowledge such as the specific technical solutions and / or characteristics in the solutions are not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A method for preparing a probiotic sanitary product, characterized in that, It includes the following preparation steps: Step A, adding ultrapure water; Step B, adding an oil agent and stirring, including the following steps: B1: Meter the oil agent and add it into the ultrapure water to form a mixed solution. The oil agent used is a hydrophilic oil agent; B2: Stir the mixed solution; Step C, cooling the mixed solution; Step D, adding probiotic powder to form a bacterial suspension, including the following steps; D1: Meter the probiotic powder and add it into the mixed solution at one time to form a bacterial suspension. The probiotic powder selected is a probiotic powder (non-viable bacteria type) added with an oil-soluble protectant; D2: Stir the bacterial suspension; Step E, coating the fiber with the bacterial suspension; Step F, using the fiber to produce probiotic sanitary products.

2. The preparation method of the probiotic sanitary product according to claim 1, wherein The step of adding ultrapure water in Step A includes: A1: Meter the ultrapure water and add it into the mixing tank; A2: Heat the mixing tank to raise the temperature of the ultrapure water to 60 - 80 °C. During the heating process, the ultrapure water needs to be continuously stirred. 3.. The preparation method of the probiotic hygiene product according to claim 2, characterized in that: In the step of adding the oil agent and stirring in Step B, the mass of the oil agent in A1 is 0.35% - 0.45% of the mass of the fiber to be coated. In the step of adding the probiotic powder, the mass of the probiotic powder in A1 is 0.5% - 2.5% of the mass of the fiber to be coated.

4. The preparation method of the probiotic sanitary product according to claim 3, characterized in that: In the step of cooling the mixed solution in Step C, the mixed solution needs to be cooled to below 50 °C. 5.. The preparation method of the probiotic hygiene product according to claim 4, characterized in that: The oil-soluble protectant used in Step D1 includes 10% linolenic acid, 15% trehalose, 5% glycerol, 6% lauryl alcohol, 2% L-arginine, 1% conjugated linolenic acid by mass fraction, and the balance is water.

6. The preparation method of the probiotic sanitary product according to claim 5, characterized in that: In the step of stirring the bacterial suspension in Step D2, the stirring speed is 6000 - 12000 rpm / min, and the stirring time is 60 - 70 min.

7. The preparation method of the probiotic hygiene product according to claim 6, characterized in that: In the step of coating the fiber with the bacterial suspension in Step E, the bacterial suspension needs to be continuously stirred, the stirring speed is 40 - 50 rpm / min, and at the same time, the bacterial suspension is heat-insulated, and the temperature is controlled at 40 - 45 °C.

8. The preparation method of the probiotic hygiene product according to claim 7, characterized in that: In the step of stirring the bacterial suspension in Step D2, the stirring speed is 10000 rpm / min, and the stirring time is 65 min.

9. The preparation method of the probiotic hygiene product according to claim 1 or 8, characterized in that: In Step F, use the fiber to produce hot air non-woven fabric, and then cover and press the hot air non-woven fabric into a sanitary napkin or a diaper to form a probiotic sanitary product. 10..A sanitary napkin for women, comprising a surface layer, a diversion layer, and a diversion core body, characterized in that: Any one or more of the surface layer, the diversion layer, and the diversion core are made of the hot air non-woven fabric in Claim 9.

Citation Information

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