Stable synergistic fungicidal composition

Abstract

The present invention relates to a stable synergistic fungicidal composition comprising fungicidal effective compounds.

Description

[0001] STABLE SYNERGISTIC FUNGICIDAL COMPOSITION

[0002] FIELD OF THE INVENTION

[0003] The present invention relates to a stable synergistic fungicidal composition comprising fungicidal effective compounds. In particular, the present invention relates to a stable synergistic fungicidal composition comprising (A) Fluopyram or its agrochemically acceptable salts, esters or its derivatives; (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and (C) another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives for synergistic and effective control of various fungal infections. The present invention further relates to process of preparing said synergistic fungicidal composition.

[0004] BACKGROUND OF THE INVENTION

[0005] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the present invention, or that any publication specifically or implicitly referenced is prior art.

[0006] Fungicides are a major component of modem agricultural practices. An agriculturalist can raise the crop's yield and quality by using fungicides, which also raises the crop's value. Fungicides are a vital and significant tool that farmers use to control the diseases. Over time, a variety of fungicides have been created with numerous advantageous qualities, including high activity at low use rates, specificity, and eradicant and curative action.

[0007] The control of phytopathogenic fungi is of great economic importance since fungal growth on plants or on parts of plants, e.g., fruits, blossoms, foliage, stems, tubers, roots, etc., inhibits production of foliage, fruit or seed and reduces the overall quantity and quality of the harvested crop. As the phytopathogenic fungi continue to cause economic harm, there is an ongoing need to create new, more potent fungicides combination with systemic, preventive, and curative effects to safeguard cultivated plants and the seeds they produce.

[0008] However, no fungicide is suitable for every situation and repeated use of a fungicide often results in resistance to that and other fungicides. As a result, research is ongoing to develop fungicides and fungicide combinations that are safer, more effective, lower dosage, simpler to use, and less expensive.

[0009] Various formulations have been developed for the control of fungi and in practice has been used as a single agent or as a mixture. For instance, international patent application WO 97 / 40683 discloses a composition of propiconazole and tricyclazole, effective for treating rice against infestation by Pyricularia species and Rhizoctonia species. However, the efficacy of this combination against the fungal rice pathogens is not yet fully satisfactory. Therefore, there is a requirement for agents that control the fungal pathogens more effectively on rice plants. Rice cultivation requires blast mitigation in order to improve global food security and reduce the losses caused by explosions.

[0010] Reference is also be made for the international patent application WO 2005 / 077901 Al and corresponding European patent EP 1751109B 1 which generally describe fungicidal compounds containing a derivative of pyridylethylbenzamide and a compound capable of preventing electron transport in the respiratory chain of phytopathogenic organisms. This reference is mainly about binary formulations, referring inter alia to the binary combination of fluopyram and bixafen.

[0011] However, no concrete combination of fluopyram with an active ingredient from the group of Strobilurin fungicides and further with another fungicide for the treatment of crops against nematodes / fungi is disclosed therein. Even more, the concrete examples solely refer to the use of fluopyram alone but not to any ternary active ingredient composition as developed by way of the present invention.

[0012] Accordingly, there is a need to develop a fungicidal combination that shows synergistic effect and are safe, effective and can be applied easily. The purpose of the present invention is to offer composition with fungicidal actives combinations that have an enhanced and / or enhanced fungicidal action and / or a wider action spectrum against Phytopathogenic fungi.

[0013] Also, the active ingredients of different mechanisms of action can be complex, some of the diseases are resistant to commonly used methods, and applications are based on the actual production of the effect. There is a need in selecting different active ingredients / agrochemically active compounds which can manifest to improve control efficiency, reducing the use of fungicides, possess broad spectrum activity and improve the germination rate, yield and plant vigour, health and are non-phy to toxic. It is always preferable to be able to reduce the amount of chemical agents released into the environment while still ensuring effective fungal disease control. Although fungus control agent combinations have been studied, a high synergistic action has not always been found.

[0014] Thus, there is a need of a fungicidal compositions for fungal disease control comprising of specific fungicides which can overcome some of the aforementioned existing problems and can be prepared easily without much complex manufacturing process.

[0015] The combating of harmful phytopathogenic fungi is in many regions not the only problems the farmers have to face, as harmful insects can cause a great damage to crops and other plants. Accordingly, there exists a need for combining fungicides belonging to different chemical classes and showing different mechanism of action and is useful various fungal diseases

[0016] Thus, there is a need of a fungicidal compositions and formulations for control of fungi and comprising of specific fungicides which can overcome some of the aforementioned existing problems and can be prepared easily without much complex manufacturing process.

[0017] It is surprisingly found that combination of below mentioned active ingredients in a specific weight percentage % range shows a synergistic effect and enhanced efficacy with reduced dosage of fungicides and is environmentally friendly, easy to formulate, stable, safe to use and has longer shelf life. This object is achieved according to the invention by providing the present composition using below mentioned components.

[0018] Fluopyram belongs to a pyridinylethylbenzamide fungicide and nematicide which has IUPAC name: N- { 2- [3 -chloro-5 -(trifluoromethyl)-2-pyridyl] ethyl } -a, a, a-trifluoro-o-toluamide, CAS name: N - [2- [3 -chloro-5-(trifluoromethyl)-2-pyridinyl] ethyl] -2-(trifluoromethyl)benzamide, CAS RN: 658066-35-4 and has an action mechanism of a succinate dehydrogenase inhibitor within the fungal mitochondrial respiration chain by binding to ubiquinone -binding sites (Qp) in the succinate dehydrogenase complex and also has nematicide activity. It is used to control botrystis, powdery mildew and other diseases. Fluopyram inhibits, the respiration of fungi and also inhibiting spore germination, extension of a germination tube, hypha growth and spore formation. It has strong and selective fungicidal action on potatoes, sugarbeet, fruit including apples, grapes and strawberries, cotton and the like. It has the chemical structure as below:

[0019]

[0020] Strobilurin fungicides are now more properly referred to as Qol fungicides. Qol fungicides are excellent as preventive fungicides, because they all effectively kill germinating spores. All Qol fungicides share a common biochemical mode of action: they all interfere with energy production in the fungal cell. They block electron transfer at the site of quinol oxidation (the Qo site) in the cytochrome bcl complex, thus preventing ATP formation. These fungicides control an unusually wide array of fungal diseases, including diseases caused by water molds, downy mildews, powdery mildews, leaf spotting and blighting fungi, fruit rotters, and rusts. They are used on a wide variety of crops, including cereals, field crops, fruits, tree nuts, vegetables, turfgrasses, and ornamentals.

[0021] Various strobilurin fungicides are known in the art. They are also categorised as systemic and translaminar strobilurin fungicides. Each of the strobilurin fungicide has different effective activity against fungal disease. Out of the known strobilurin fungicides, Azoxystrobin and Trifloxystrobin have shown to have better fungal disease control in comparison to other strobilurin fungicides.

[0022] Azoxystrobin belongs to class of methoxyacrylate fungicide having a chemical name as methyl (2E)-2-(2-{ [6-(2-cyanophenoxy)pyrimidin-4-yl]oxy}phenyl)-3-methoxyprop-2-enoate or methyl (2E)-2-(2-{ [6-(2-cyanophenoxy)pyrimidin-4-yl]oxy}phenyl)-3-methoxyacrylate.

[0023] Azoxystrobin Azoxystrobin is a strobilurin fungicide, commonly used in agriculture. Azoxystrobin possesses the broadest spectrum of activity of all known antifungals. The substance is used as an active agent protecting plants and fruit / vegetables from fungal diseases. Azoxystrobin binds very tightly to the Qo site of Complex III of the mitochondrial electron transport chain, thereby ultimately preventing the generation of ATP. Azoxystrobin is widely used in farming, particularly in wheat farming.

[0024] Trifloxystrobin belong to the oximinoacetate class of fungicide, having chemical name as methyl (2E)-(methoxyimino)(2- { [( { ( 1 E) - 1 - [ 3 -

[0025] (trifluoromethyl)phenyl]ethylidene]amino)oxy]methyl}phenyl)acetate or methyl (E)- (methoxyimino) { a-( { [(E)- 1 -(a, a, a-trifluoro-m-tolyl)ethylidene] amino } oxy iotolyl] acetate.

[0026] T rifloxystrobin

[0027] Trifloxystrobin is a foliar applied fungicide for cereals which is particularly active against Ascomycetes, Deuteromycetes and Oomycetes. It has a broad spectrum of action with preventative and curative action. It is a respiration inhibitor (QoL fungicide).

[0028] Another fungicides include Difenoconazole or Captan or Cyazofamid apart from other azole compounds.

[0029] Difenoconazole belongs to the triazole class of fungicide and have a chemical name as l-[[2- [2-chloro-4-(4-chlorophenoxy)phenyl]-4-methyl- 1 , 3 -dioxolan-2-yl] methyl] - 1H- 1 ,2,4-triazole or 3-chloro-4-[(2RS ,4RS ;2RS ,4SR)-4-methyl-2-( 1H- 1 ,2,4-triazol- 1 -ylmethyl)- 1 ,3 -dioxolan- 2-yl]phenyl 4-chlorophenyl ether.

[0030]

[0031] Difenoconazole is a broad-spectrum fungicide that controls a wide variety of fungi - including members of the Aschomycetes, Basidomycetes and Deuteromycetes families. It acts as a seed treatment, foliar spray and systemic fungicide. It is taken up through the surface of the infected plant and is translocated to all parts of the plant. It has a curative effect and a preventative effect. Difenoconazole can be applied to winter wheat, oilseed rape, Brussels sprouts, cabbage, broccoli / calabrese and cauliflower.

[0032] Captan belongs to phthalimide class of fungicides and has a chemical name as N- [(trichloromethyl)thio]-3a,4,7,7a-tetrahydrophthalimide or 3a,4,7,7a-tetrahydro-N- [(trichloromethyl)thio]phthalimide. Captan is a fungicide used on fruits, vegetables, and ornamentals.

[0033] Cyazofamid belongs to the cyanoimidazole class of fungicide having a chemical name as 4- chloro-2-cyano-N,N-dimethyl-5-p-tolylimidazole-l-sulfonamide.

[0034]

[0035] Cyazofamid is a fungicide used mainly for controlling Oomycete and Plasmodiophora diseases on potatoes and tomatoes. Its mode of action is through preventing foliar and soil action with some residual activity and Respiration inhibitor effects.

[0036] Therefore, the present invention aims to develop a stable synergistic fungicidal composition, which results in broader fungicidal spectrum of action. Thus, the present invention provides a composition for protecting plants, crops against fungal diseases.

[0037] The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.

[0038] The present invention composition is synergistic, efficacious, environmentally friendly, simple to formulate, stable, safe to use and has longer shelf life.

[0039] OBJECTS AND ADVANTAGES OF THE INVENTION:

[0040] The principal object of the present invention is to provide a novel and effective stable synergistic fungicidal composition demonstrating high efficacy and to provide complete protection to crops, plants against fungal diseases.

[0041] It is an object of the present invention to provide a novel and effective stable synergistic fungicidal composition demonstrating high efficacy and high selectivity.

[0042] It is an object of the present invention to provide improved combinations of fungicidal compounds for broad spectrum control of fungal diseases.

[0043] It is another object of the present invention to provide a method and a stable synergistic composition for controlling fungal diseases. It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition which has both preventive and curative action and is active against all life stages of major plant pathogens.

[0044] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition that saves crop & environmental damage due to reduced application rate and dose.

[0045] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition that provides increased crop yield and / or improved plant health.

[0046] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition that improves quality and quantity of the targeted crops.

[0047] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition which is ideal for fungi resistance management and enhanced disease control.

[0048] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition which uses lesser amounts of the actives in the composition as compared to the actives when used alone.

[0049] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition which is environmentally safe, possesses broad spectrum bio-efficacy and is not toxic in terms of phyto toxicity.

[0050] It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is stable at various temperature ranges and variable environmental changes.

[0051] It is another object of the present invention to provide a novel and effective stable synergistic fungicidal composition which has enhanced efficacy and penetration capacity.

[0052] Yet another object of the present invention is to provide a novel and effective stable synergistic fungicidal composition which promotes plant health. Another object of the present invention is to provide a novel and effective stable synergistic fungicidal composition which reduce the number of sprayings by providing longer duration control of fungal disease.

[0053] It is another object of the present invention to provide a novel and stable synergistic fungicidal composition which can be easily formulated.

[0054] A further object of the present invention is to provide a novel and effective stable synergistic fungicidal composition and a method of controlling pathogenic fungus and for disease resistance management or to delay disease resistance development through engaging multiple modes of action.

[0055] A further object of the present invention is to provide a novel and effective synergistic fungicidal composition which has increased shelf life.

[0056] Embodiment of the present invention can ameliorate one or more of the above-mentioned problems.

[0057] The stable synergistic fungicidal composition of the present invention is found to be useful in protecting a wide range of crops like fruits, vegetables, cereals, flowers etc. against major plant diseases. The present composition achieves improved biological efficacy by enhancing overall control of plant / crop fungal disease over a shorter period of time. Additional benefits of using the fungicidal composition of the present invention include reduced risk of occupational hazard, lower cost of application, better cost: benefit ratio to the end user.

[0058] Another object of the present invention is to provide a stable synergistic fungicidal composition comprising (A) Fluopyram or its agrochemically acceptable salts, esters or its derivatives; (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and (C) another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

[0059] Yet another object of the present invention is to provide a method of improving plant health and increasing crop yield by using stable synergistic fungicidal composition comprising (A) Fluopyram or its agrochemically acceptable salts, esters or its derivatives; (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and (C) another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

[0060] Some or all these and other objects of the invention can be achieved by way of the invention described hereinafter.

[0061] SUMMARY OF THE INVENTION

[0062] Accordingly, the main aspect of the present invention is to provide a stable synergistic fungicidal composition having high fungus control efficacy.

[0063] Accordingly, the present invention relates to a stable synergistic fungicidal composition comprising:

[0064] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;

[0065] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and

[0066] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, for synergistic and effective control of various fungal disease.

[0067] In yet another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0068] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;

[0069] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives;

[0070] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives; and

[0071] (D)One or more agrochemically acceptable excipients.

[0072] In yet another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0073] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition; (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0074] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0075] In yet another embodiment of the present invention, the synergistic fungicidal composition comprises:

[0076] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0077] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0078] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition and

[0079] (D)One or more agrochemically acceptable excipients.

[0080] In one another preferred embodiment of the present invention, the synergistic fungicidal composition comprises:

[0081] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-85% by weight of the composition;

[0082] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-85% by weight of the composition;

[0083] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-85% by weight of the composition.

[0084] In one another embodiment of the present invention, provides a method of improving plant health and increasing crop yield by using stable synergistic fungicidal composition comprising:

[0085] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives; (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives;

[0086] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

[0087] In one another embodiment of the present invention, there is also a process provided for the preparation of stable synergistic fungicidal composition comprising:

[0088] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;

[0089] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives;

[0090] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

[0091] In one embodiment of the present invention, the present fungicidal composition is stable and has enhanced shelf life.

[0092] In another aspect, the present invention provides a synergistic fungicidal composition for controlling various fungus in field crops, and effective controlling wide range of nematicides and / or fungus.

[0093] In an aspect, the present invention provides a synergistic fungicidal composition which has enhanced efficacy and penetration capacity.

[0094] DETAILED DESCRIPTION OF THE INVENTION

[0095] In describing the embodiments of the invention, specific terminology is resorted for sake of clarity. However, it is not intended that the invention be limited to specific terms so selected and it is to be understood that each specific term includes all technical equivalents that operate in a similar manner to accomplish a similar purpose.

[0096] Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods.

[0097] All technical and scientific terms used herein have the same meanings as commonly understood by someone ordinarily skilled in the art to which the present subject matter belongs. The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure.

[0098] The expression of various quantities in terms of “% w / w” or “%” means the percentage by weight, relative to the weight of the total formulation or composition unless otherwise specified.

[0099] The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control and killing of fungal diseases.

[0100] The term “crop” shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

[0101] The term “synergistic”, as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.

[0102] “Bioactive amounts” as mentioned herein means that amount which, when applied for treatment of crops, is sufficient to give effect in such treatment.

[0103] The term “health of a plant” or “plant health” is defined as a condition of the plant and / or its products. As a result of the improved health; yield, plant vigour, quality and tolerance to abiotic or biotic stress is increased. As a result, the health of a plant is increased even in the absence of pest pressure.

[0104] Accordingly, in an especially preferred embodiment according to the invention, the health of a plant is increased both in the presence and absence of biotic or abiotic stress factors. An increase in plant vigor may for example result in an increased yield and / or tolerance to abiotic or biotic stress.

[0105] One indicator for the condition of the plant is the yield. “Yield” is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals). The plant products may in addition be further utilized and / or processed after harvesting.

[0106] In an especially preferred embodiment of the invention, the yield of the treated plant is increased. In another preferred embodiment of the invention, the yield of the plants treated according to the method of the invention, is increased synergistically.

[0107] In another especially preferred embodiment of the invention, the plant vigour of the treated plant is increased. In another preferred embodiment of the invention, the plant vigour of the plants treated according to the composition of the present invention is increased synergistically.

[0108] Another indicator for the condition of the plant is the “quality” of a plant and / or its products.

[0109] In an especially preferred embodiment of the invention, the quality of the treated plant is increased.

[0110] In another preferred embodiment of the invention, the quality of the plants treated according to the method of the invention, is increased synergistically.

[0111] The present invention includes within its scope, all possible salts, esters and derivatives of the active ingredients of the composition.

[0112] It has been observed by the inventors of the present invention that the composition of present invention not only has enhanced bio-efficacy and provides synergistic effect but also gives control of diseases caused by fungi for a longer duration as compared to solo and binary formulations of fungicides.

[0113] In one embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0114] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;

[0115] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and

[0116] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

[0117] In yet another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0118] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;

[0119] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives; and

[0120] (D)One or more agrochemically acceptable excipients.

[0121] In another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0122] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0123] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition; and

[0124] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0125] In one another preferred embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0126] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-85% by weight of the composition;

[0127] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-85% by weight of the composition;

[0128] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-85% by weight of the composition and

[0129] (D)One or more agrochemically acceptable excipients.

[0130] In one another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0131] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0132] (B) Trifloxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and (C) Difenoconazole or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0133] In one another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0134] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0135] (B) Azoxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and

[0136] (C) Difenoconazole or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0137] In one another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0138] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0139] (B) Trifloxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and

[0140] (C) Captan or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0141] In one another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0142] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0143] (B) Azoxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and

[0144] (C) Captan or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0145] In one another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0146] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition; (B) Trifloxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and

[0147] (C) Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0148] In one another embodiment of the present invention, the stable synergistic fungicidal composition comprises:

[0149] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;

[0150] (B) Azoxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and

[0151] (C) Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0152] In another embodiment, the present invention provides a stable synergistic fungicidal composition, further comprises one or more agrochemically acceptable excipients.

[0153] In another embodiment, “agrochemically acceptable excipients” are substances used in agrochemical formulation that are compatible with the active substance and do not negatively impact the efficacy or safety of the active ingredient and do not pose any risk to the environment and health of non-target organisms.

[0154] In yet another embodiment, the present invention provides a kit-of-parts comprising a plurality of components, wherein said plurality of components comprises:

[0155] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;

[0156] (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and

[0157] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

[0158] In one another embodiment of the present invention, provides a method of improving plant health and increasing crop yield by using synergistic fungicidal composition comprising:

[0159] (A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition; (B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition; and

[0160] (C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

[0161] In an embodiment, the present invention provides a method for controlling a plant disease caused by a plant-pathogens comprising applying to the plant or portion thereof or soil, a fungicidal effective amount of the fungicidal composition.

[0162] In an embodiment, the present invention provides a process for preparing a stable synergistic fungicidal composition.

[0163] In an embodiment, the present invention provides a process for preparing a stable synergistic fungicidal composition.

[0164] In another embodiment, the synergistic insecticidal composition comprises:

[0165] • 30 % w / w of Fluopyram, 2% w / w of Trifloxystrobin, 17% w / w of Difenoconazole and one or more excipients;

[0166] • 24 % w / w of Fluopyram, 16% w / w of Trifloxystrobin, 2% w / wof Difenoconazole and one or more excipients;

[0167] • 16.4 % w / w of Fluopyram, 7.2% w / w of Trifloxystrobin, 9.6 % w / w of Difenoconazole and one or more excipients;

[0168] • 6 % w / w of Fluopyram, 12% w / w of Trifloxystrobin, 24% w / w of Difenoconazole and one or more excipients;

[0169] • 23 % w / w of Fluopyram, 0.1% w / w of Azoxystrobin, 30% w / w of Difenoconazole and one or more excipients;

[0170] • 14.4 % w / w of Fluopyram, 12.4% w / w of Azoxystrobin, 8% w / w of Difenoconazole and one or more excipients;

[0171] • 1% w / w of Fluopyram, 30% w / w of Azoxystrobin, 16% w / w of Difenoconazole and one or more excipients;

[0172] • 33% w / w of Fluopyram, 22% w / w of Azoxystrobin, 0.1% w / w of Difenoconazole and one or more excipients; • 35% w / w of Fluopyram, 16% w / w of Trifloxystrobin, 5% w / w of Captan and one or more excipients;

[0173] • 7.2% w / w of Fluopyram, 3% w / w of Trifloxystrobin, 41.8% w / w of Captan and one or more excipients;

[0174] • 17% w / w of Fluopyram, 28% w / w of Trifloxystrobin, 19% w / w of Captan and one or more excipients;

[0175] • 0.1 % w / w of Fluopyram, 0.1% w / w of Trifloxystrobin, 55% w / w of Captan and one or more excipients;

[0176] • 14% w / w of Fluopyram, 13% w / w of Azoxystrobin, 20% w / w of Captan and one or more excipients;

[0177] • 11% w / w of Fluopyram, 10% w / w of Azoxystrobin, 54% w / w of Captan and one or more excipients;

[0178] • 7.2% w / w of Fluopyram, 6.2% w / w of Azoxystrobin, 41.6% w / w of Captan and one or more excipients;

[0179] • 3% w / w of Fluopyram, 2% w / w of Azoxystrobin, 70% w / w of Captan and one or more excipients;

[0180] • 30% w / w of Fluopyram, 2% w / w of Trifloxystrobin, 18% w / w of Cyazofamid and one or more excipients;

[0181] • 24% w / w of Fluopyram, 16%w / w of Trifloxystrobin, 2% w / w of Cyazofamid and one or more excipients;

[0182] • 16.4% w / w of Fluopyram, 7.2%w / w of Trifloxystrobin, 10.4% w / w of Cyazofamid and one or more excipients;

[0183] • 6% w / w of Fluopyram, 12%w / w of Trifloxystrobin, 24% w / w of Cyazofamid and one or more excipients;

[0184] • 28% w / w of Fluopyram, 5%w / w of Azoxystrobin, 20% w / w of Cyazofamid and one or more excipients;

[0185] • 22% w / w of Fluopyram, 20%w / w of Azoxystrobin, 2% w / w of Cyazofamid and one or more excipients;

[0186] • 14.4% w / w of Fluopyram, 12.4%w / w of Azoxystrobin, 9.2% w / w of Cyazofamid and one or more excipients;

[0187] • 6% w / w of Fluopyram, 26% w / w of Azoxystrobin, 15% w / w of Cyazofamid and one or more excipients; In accordance with an embodiment of the present invention, there is provided a stable synergistic fungicidal composition comprising active ingredients as given below:

[0188] In one aspect / another embodiment, the stable synergistic fungicidal composition of present invention can be applied to a plant / crop / soil by spraying, rubbing, atomizing, irrigating, evaporating, dusting, fogging, broadcasting, pouring, mist blowing, soil mixing, foaming, painting, spreading-on, drenching, dipping or drip irrigation, etc.

[0189] In yet another embodiment of the present invention, the present stable synergistic fungicidal composition can be applied to various crops and plants including but not limited to grapes, chilli, mango, tomato, potato, cucumber, cumin, pomegranate, wheat, rice, apple, onion, groundnut, cherry, cabbage, etc.

[0190] The composition of the present invention can be used to control the various fungal diseases including but not limited to Root knot nematode (Meloidogyne incognita), Sheath blight, Loose smut, Scab, Fly speck and Bitter rot, Downy mildew, Powdery mildew, Anthracnose, Early blight and Late blight, Purple blotch, Late Blight of potato, Fruit rot, Powdery mildew, Anthracnose, Damping off (soil drench), Die -back, Fruit rot, Blight mildew, Leaf spot, Fruit spot, Fruit rot, rust, Damping off (Nursery), Brown rot etc.

[0191] The composition according to the invention can be applied to any and all developmental stages of fungus development. The fungus disease may be controlled by contacting the target fungi, its food supply, habitat, breeding ground or its locus with a bioactive amount of the present composition.

[0192] The novel composition of the present invention has very advantageous, curative, preventive and systemic fungicidal properties for protecting cultivated plants and crops. As has been mentioned, said active ingredient composition can be used to inhibit or destroy the pathogens that occur on plants or parts of plants (fruit, blossoms, leaves, stems, tubers, roots) of different crops or useful plants, while at the same time those parts of plants which grow later are also protected from attack by such pathogens.

[0193] The composition of the present invention also provides control of diseases caused by a broad spectrum of fungal plant pathogens preventatively or curatively by applying an effective amount of the composition either pre-or post-infection.

[0194] Plant disease control is ordinarily accomplished by applying an effective amount of a synergistic composition of the present invention either pre-or post-infection, to the portion of the plant to be protected such as the roots, stems, foliage, fruit, seeds, tubers or bulbs, or to the media (soil or sand) in which the plants to be protected are growing. The composition can also be applied to the seed to protect the seed and seedling.

[0195] The composition of the present invention is effective in controlling broad spectrum of insect but not limited to Root knot nematode (Meloidogyne incognita), Sheath blight, Loose smut, Scab, Fly speck and Bitter rot, Downy mildew, Powdery mildew, Anthracnose, Early blight and Late blight, Purple blotch, Late Blight of potato, Fruit rot, Powdery mildew, Anthracnose, Damping off (soil drench), Die -back, Fruit rot, Blight mildew, Leaf spot, Fruit spot, Fruit rot, rust, Damping off (Nursery), Brown rot etc. including fungus resistant to chemicals, and shows excellent controlling effects (synergistic controlling effects) which could never be expected from a single component alone.

[0196] In yet another embodiment of the present invention, the present stable synergistic fungicidal composition can be formulated as one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro -emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) or a mixed formulation of CS and EW (ZW).

[0197] In yet another embodiment of the present invention, the stable synergistic fungicidal composition is preferably formulated either as Suspension concentrate (SC) or Water dispersible granules (WG) or Wettable powder (WP).

[0198] In another embodiment of the present invention, the stable synergistic fungicidal composition of the present invention further comprises one or more agrochemically acceptable excipients including but not limited to the group comprising surfactant(s), dispersing agent(s), codispersant anti-freezing agent(s), emulsifier(s), anti-foaming agent / defoamer(s), wetting agent(s), antimicrobial / anti-bacterial agent(s), thickening agent / thickener(s), quick coating agent(s) or sticking agents / sticker(s), spreader(s), binder(s), pH-adjusting agent(s), anti-caking agent(s), adjuvant(s), filler(s) / suspension aid(s), colorant(s), carrier(s), buffering agent(s), polymer(s), rheology modifier(s), solvent(s), co-solvent(s) or a combination thereof. Additional components may also be included, e.g., protective colloids, carriers, adhesives, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active materials can be combined with any solid or liquid carrier / additive, which complies with usual formulation techniques.

[0199] An adjuvant used in the present invention is any material that is added to an agrochemical formulation to enhance or modify the performance of the formulation. An adjuvant is used in the present invention to make it a safer to ecological environmental, having low toxicity and having no phytotoxic effects on any part of the plant.

[0200] Examples of surfactant(s) and / or dispersing agent(s) used herein include but not limited to Polymeric dispersant, polymeric anionic dispersant, polyarylphenyl ether phosphate, tristyrylphenol ethoxylate & craft copolymer, Polymethyl methacrylate -polyethylene glycol graft copolymer, acrylate copolymer, acrylic graft copolymer, non-ionic proprietary surfactant, block copolymer , ethoxylated tristryl phenol Sulphate, alkyl naphthalene formaldehyde condensate , naphthalene sulfonic acid, sodium salt condensate with formaldehyde, alkyl naphthalene sulfonic acid, methyl naphthalene sulfonic acid , polymer with formaldehyde sodium salt, ethoxylated oleyl cetyl alcohol, polyalkelene glycol ether, ethoxylated fatty alcohol, Sodium polymethyl methacrylate, polyacrylate, sodium polyacrylate, sodium lignosulphonate, calcium lignosulphonate, alkylphenol polyoxyethylene ether, Sodium salt of naphthalene sulfonate condensate , sodium salt of alkyl naphthalene sulfonate condensate, alkyl or polyalkyl naphthalene sulphonate or its sodium salt, Sodium alkyl naphthalene sulfonate, Sodium Isopropyl Naphthalene Sulfonate, Sodiumdiisopropyl naphthlene sulphonate, sodium salt of alkyl or polyalkyl naphthalene sulfonate formaldehyde condensate, polyalkyl naphthalene sulphonate, Amine Salt Of a Phosphated Tristyryl Phenol Ethoxylate, polycarboxylate, Polyalkoxy ethers , fatty alcohol polyoxyethylene ether, alcohol polyglycol ether or mixtures thereof and present in the range of 0.1-20% by weight of the composition.

[0201] Examples of wetting agent(s) used herein include but not limited to tristyrylphenol ethoxylate non-ionic emulsifier, mixture of non-ionic surfactants, alkoxylated alcohol, block copolymer, alcohol polyglycol ether, alkyl olefin sulphates, dioctyl sulpho succinate, alkyl naphthalene sulphate, polyalkyl naphthalene sulphonate, alkyl phenol ethoxylates, tri-styrenated phenol ethoxylate, EO / PO block co-polymers, sodium lauryl sulphate, sodium ligno sulphates, Ethanol 2,2,2 -nitrilotris with alpha-(2,4,6-tris(l-phenylethyl)phenyl)- omega-hydroxypoly(oxy-l,2- ethanediyl) phosphate, Amine Salt Of a Phosphated Tristyryl Phenol Ethoxylate, Polyethylene Polypropylene glycol mono butyl ether , Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide or mixtures thereof and present in the range of 0.1-8.0 % by weight of the total composition.

[0202] Examples of anti-foaming agent / defoamer(s) used herein include but not limited to silicon emulsion based anti-foam agents, Siloxane polyalkyleneoxide, Polydimethylsiloxane emulsion, trisiloxane ethoxylates, silicone oil, silicone compound, C10~C20 saturated fat acid compounds or C8~C10 aliphatic alcohols compound, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide modified polydimethylsiloxane and mixtures thereof and present in the range of 0.01-5.0% by weight of the composition.

[0203] Examples of anti-freezing agent(s) used herein include but not limited to glycol, propylene glycol, mono ethylene glycol, Glycerol, glycerine, diethylene glycol, sorbitol, urea, mannitol, polyethylene glycol or mixtures thereof and present in the range of 0.1-12 % by weight of the composition. Examples of antimicrobial / anti -bacterial agent(s) used herein include but not limited to 1,2- benzisothiazolin-3-one, formaldehyde, sodium benzoate / Sodium o-phenylphenate, 5-chloro-2- methyl-4-isothiazolin-3-one & 2- methyl-4-isothiazolin-3-one, potassium sorbate or mixtures thereof and present in the range of 0.01-8.0 % by weight of the composition.

[0204] Examples of solvent(s) used herein include but not limited to heavy aromatic hydrocarbon, N- methyl pyrollidone(NMP), Di-methyl sulfoxide(DMSO), Solvent C-IX, N- alcohol, alkyl amide, vegetable oil, mineral oil, distilled water, aromatic solvents, water or mixtures thereof and present in the range of 0.1-20 % by weight of the composition.

[0205] Examples of emulsifier(s) used herein include but not limited to nonionic surfactant of ethoxylated fatty acid type, such as ethoxylated fatty acid mono-, di- or triglycerides or ethoxylated dialkyl sulfosuccinate, Tri-styrenated phenol ethoxylate , Emulsifier (Blend of Anionic and Non-ionic), blend of Calcium Alkyl benzene sulphonate and Tri-styrenated phenol ethxylate an alkylaryl sulfonate, a polyoxyethylene fatty acid ester, a polyoxyethylene sorbitol fatty acid ester, a polyoxyethylene castor oil and a polyoxyethylene hydrogenated castor oil., ethoxylated fatty alcohol type or a block copolymer comprising one or more alkylene oxide block type or mixtures thereof and present in the range of 0.1-25 % by weight of the composition

[0206] Rheology modifiers are sometimes referred to as thickeners or thickening agents, but they do much more than just thicken a formulation. A good rheology modifier structures the formulation but when a force is applied it becomes flowable and easily poured.

[0207] Examples of rheology modifier or thickening agent / thickener(s) or filler(s) used herein include but not limited to selected from the group comprising polysaccharides, Lactose monohydrate, carboxymethyl cellulose, bentonite clay, china clay, kaolin, aluminium magnesium silicate, hydroxy propyl cellulose, xanthan gum, gum arabic, gaur gum, silica powder, carbomer, chitosan, silica, precipitated silica or mixtures thereof and present in the range of 0.01-15.0 % by weight of the composition.

[0208] In an embodiment, the kit-of-parts comprises an instructions manual, said instructions manual comprising instructions directing a user to ad-mix the components before being used. The process for preparing the present novel synergistic composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However, all such variation and modification are still covered by the scope of present invention.

[0209] Thus, before describing the present invention in detail, it is to be understood that this invention is not limited to particularly exemplified systems or process parameters that may of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control.

[0210] The composition of the present invention can also comprise or may be applied together and / or sequentially with further active compounds. These further compounds can be selected from fertilizers or micronutrient donors or microorganisms or other preparations that influence plant growth, such as inoculants (e.g., a strain of nitrogen-fixing bacteria), and plant inducers.

[0211] The present invention includes within its scope, all possible salts, esters and derivatives of the active ingredients of the composition.

[0212] In one embodiment, the composition according to the present invention acts synergistically to control of fungal disease in various crops.

[0213] Through the fungicidal composition of present invention, the number of applications to control wide range of fungal disease appearing at the same time is minimised. The composition of present invention is highly safe to the user and to the environment. The composition offers the user a single homogenous application eliminating the need for tank mix. The composition also is cost-effective as it provides much greater simultaneous control and can be used in a variety of crops with a broader spectrum of protection. Representative examples of the present composition of this invention are provided below and are not to be construed as limiting in any manner:

[0214] Examples: General Recipe

[0215] Example 1. Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC

[0216] CHEMICAL COMPOSITION: FORMULATION PROCESS: 1. The mixture of surfactants (Acrylic graft copolymer, Non-ionic proprietary surfactant), Polydimethylsiloxane emulsion & Propylene Glycol (PG) were first diluted in required D.M.Water, and solubilized by high shear mixing.

[0217] 2. Then to the above, added Fluopyram Technical, Trifloxystrobin Technical, and Difenoconazole Technical mix and made the homogeneous mass.

[0218] 3. The above mixed mass was grinded in bead mill. Grinding was carried out until a mean particle size of below 5 microns was obtained.

[0219] 4. Prepared 2% gum solution - Took 100 g DM water added 0.2 g of 1,2-Benzisothiazolin- 3 -one, then under slow stirring added 2 g xanthan gum. 5. To the grinded mass, added 2% water solution of xanthan gum under low stirring and mixed till homogeneous solution was achieved.

[0220] 6. Finally checked for quality parameter.

[0221] Stability Data: Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC

[0222] Example 2. Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% WG

[0223] CHEMICAL COMPOSITION: PREPARATION PROCESS:

[0224] A. Formulation process

[0225] 1. Took Keolin in the ribbon blender and started mixing. To this added silica, Sodium alkyl naphthalene sulfonate, Sodium alkyl naphthalene sulfonate formaldehyde condensate, polyacrylate, Fluopyram technical, Trifloxystrobin technical and Difenoconazole technical and mixed well. 2. Continued to mix this till the material was completely homogenised.

[0226] B. Grinding process

[0227] 1. Allowed to pass the premix through the Airjet mill at 6 to 7 kg / cm2of grinding pressure and 100 to 150 kg / hr feeding rate so as get a particle size of D90 less than 10 micron. The grinded material was collected in post blender.

[0228] 2. WP is then checked for Al content and suspensibility parameter.

[0229] 3. After approval from QC the process was taken further for extrusion and drying.

[0230] C. Extrusion process

[0231] 1. Into the post blender the solution of water and Polydimethylsiloxane was added by means of spraying and dough was prepared. This dough was mixed well to get a well hydrated dough for extrusion.

[0232] 2. The dough was then allowed to pass through the screw valve fitted with post blender and then passed through basket extruder (Sieve size of 0.8 mm / 1.0 mm).

[0233] D. Drying Process

[0234] 1. These wet extruded granules were then passed through VFBG (vibro fluid bed drier) which was brought to a consistent temperature of 50°C prior for drying of wet granules.

[0235] 2. These granules are checked for moisture content which should not more than 3%.

[0236] 3. Once the parameter got set then the process continued.

[0237] E. Sieving and packing

[0238] The dried granule was passed through vibro siever machine to separate any lumps or dust and desired size granules were collected and packed.

[0239] STABILITY DATA:_Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6%WG

[0240]

[0241] Example 3 Fluopyram 14.4% + Azoxystrobin 12.4% + Difenoconazole 8% SC

[0242] CHEMICAL COMPOSITION: FORMULATION PROCESS:

[0243] 1. The mixture of surfactants (Acrylic graft copolymer, Methyl naphthalene sulfonic acid, polymer with formaldehyde, sodium salt), Poly dimethylsiloxane emulsion & Propylene Glycol (PG) were first diluted in required D.M.Water, and solubilized by high shear mixing.

[0244] 2. To the above solution, then added Fluopyram Technical, Azoxystrobin Technical, and Dif enoconazole Technical mix to make homogeneous mass.

[0245] 3. The above mixed mass was then grinded in bead mill. Grinding was carried out till a mean particle size of below 5 microns was obtained.

[0246] 4. Preparations of 2% gum solution - Took 100 g DM water and added 0.2 g of 1,2- Benzisothiazolin-3-one then added 2 g xanthan gum under slow stirring.

[0247] 5. After grinding, added 2% water solution of xanthan gum under low stirring. Mixed the solution well till the right viscosity was attained.

[0248] 6. Finally checked for quality parameters.

[0249] STABILITY DATA: Fluopyram 14.4% + Azoxystrobin 12.4 % + Dif enoconazole 8% SC

[0250] Example 4: Fluopyram 14.4% + Azoxystrobin 12.4% + Difenoconazole 8% WG

[0251] CHEMICAL COMPOSITION: PREPARATION PROCESS:

[0252] A. Manufacturing process

[0253] 1. Took filler (China clay) in the blender for mixing. To this added Silica powder, Sodiumdiisopropyl naphthlene sulphonate, Sodium Lauryl Sulphate, Sodium lignosulphonate, sodium salt of naphthalene sulfonate condensate, Lactose monohydrate, Fluopyram technical, Azoxystrobin technical, Difenoconazole technical and mixed well.

[0254] 2. Continued to mix till the mixture was completely homogenised.

[0255] 3. Allowed to pass the premix through airjet mill at 6-8 (kg / cm2) of grinding pressure and 2-3 (kg / cm2) feeding rate so as to get a particle size of desired microns.

[0256] 4. The grinded material was collected in post blender.

[0257] B. Extrusion process

[0258] 1. Into the post blender the solution of water and Polydimethylsiloxane emulsion was added by means of spraying and dough was prepared.

[0259] 2. This dough was mixed well to get a well hydrated dough for extrusion. This dough then allowed to pass through the screw valve fitted with post blender and then passed through basket extruder.

[0260] C. Drying process

[0261] 1. These wet extruded granules then passed through VFBG (vibro fluid bed drier) which was brought to a consistent temperature of 50 °C prior to drying of wet granules.

[0262] 2. These granules were checked for moisture content which should not be more than 3%. Once the parameter got set then the process continued.

[0263] D. Sieving and packaging

[0264] The dried granules were passed through vibro siever machine to separate any lumps or dust and desired size granules were collected and packed.

[0265] STABILITY DATA:_Fluopyram 14.4% + Azoxystrobin 12.4% + Difenoconazole 8% WG

[0266] Example 5. Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WG

[0267] CHEMICAL COMPOSITION:

[0268] PREPARATION PROCESS:

[0269] A. Formulation process

[0270] 1. Took china clay in the ribbon blender and started mixing. To this added silica powder, Sodium alkyl naphthalene sulfonate formaldehyde condensate, Sodium lauryl sulphate, sodium lignosulphate, Sodium salt of naphthalene sulfonate condensate, Polymeric dispersant, lactose monohydrate, Fluopyram technical, Trifloxystrobin technical and Captan technical and mixed well.

[0271] 2. Continued to mix this till the material is completely homogenised.

[0272] B. Grinding process

[0273] 1. Allowed to pass the premix through the Airjet mill at 6 to 7 kg / cm2of grinding pressure and 100 to 150 kg / hr feeding rate so as get a particle size of D90 less than 10 micron. The grinded material was collected in post blender.

[0274] 2. WP was then checked for Al content and suspensibility parameter.

[0275] 3. After approval from QC the process was taken further for extrusion and drying.

[0276] C. Extrusion process

[0277] 1. Into the post blender the solution of water and Polydimethylsiloxane was added by means of spraying and dough was prepared. This dough was mixed well to get a well hydrated dough for extrusion.

[0278] 2. The dough was then allowed to pass through the screw valve fitted with post blender and then passed through basket extruder (Sieve size of 0.8 mm / 1.0 mm). D. Drying Process

[0279] 1. These wet extruded granules were then passed through VFBG (vibro fluid bed drier) which was brought to a consistent temperature of 50°C prior to drying of wet granules.

[0280] 2. These granules were checked for moisture content which should not more than 3%. 3. Once the parameter got set then the process is continued.

[0281] E. Sieving and packing

[0282] The dried granule was passed through vibro siever machine to separate any lumps or dust and desired size granules are collected and packed. STABILITY DATA: Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WG Example 6. Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP

[0283] CHEMICAL COMPOSITION:

[0284] FORMULATION PROCESS:

[0285] 1. Blend the Al’s Fluopyram, Trifloxystrobin and Captan with inert fillers china clay, silica powder, Polydimethylsiloxane emulsion and dispersing agents Sodium Isopropyl Naphthalene Sulfonate to aid in dispersion and wetting.

[0286] 2. Grind the mixture to reduce the particle size, aiming for fine particles (10-50 microns) for better dispersion.

[0287] 3. Added Sodium ligno sulphonate and Sodium lauryl sulphate to improve the powder's ability to disperse in water.

[0288] 4. If needed, granulate the powder to improve flow and handling.

[0289] 5. Removed the moisture to prevent clumping and ensure stability.

[0290] 6. Finally Sieved the powder to achieve uniform particle size.

[0291] STABILITY DATA: Fluopyram 7.2% + Trifloxystrobin 3 % + Captan 41.8% WP

[0292] Example 7: Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WG

[0293] CHEMICAL COMPOSITION:

[0294] PREPARATION PROCESS:

[0295] A. Formulation process

[0296] 1. Took china clay in the ribbon blender and started mixing. To this added silica powder, Sodiumdiisopropyl naphthlene sulphonate, Sodium ligno sulphonate, Sodium salt of naphthalene sulfonate condensate, Acrylate Copolymer lactose monohydrate, Fluopyram technical, Azoxystrobin technical and Captan technical and mixed well.

[0297] 2. Continued to mix this till the material is completely homogenised.

[0298] B. Grinding process

[0299] 1. Allowed to pass the premix through the Airjet mill at 6 to 7 kg / cm2of grinding pressure and 100 to 150 kg / hr feeding rate so as get a particle size of D90 less than 10 micron. The grinded material was collected in post blender.

[0300] 2. WP was then checked for Al content and suspensibility parameter.

[0301] 3. After approval from QC the process was taken further for extrusion and drying.

[0302] C. Extrusion process

[0303] 1. Into the post blender the solution of water and Polydimethylsiloxane was added by means of spraying and dough was prepared. This dough was mixed well to get a well hydrated dough for extrusion.

[0304] 2. The dough was then allowed to pass through the screw valve fitted with post blender and then passed through basket extruder (Sieve size of 0.8 mm / 1.0 mm). D. Drying Process

[0305] 1. These wet extruded granules were then passed through VFBG (vibro fluid bed drier) which was brought to a consistent temperature of 50°C prior to drying of wet granules. 2. These granules were checked for moisture content which should not more than 3%.

[0306] 3. Once the parameter got set then the process is continued.

[0307] E. Sieving and packing

[0308] The dried granule was passed through vibro siever machine to separate any lumps or dust and desired size granules are collected and packed.

[0309] STABILTY DATA: Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WG

[0310] Example 8. Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP

[0311] CHEMICAL COMPOSITION: FORMULATION PROCESS:

[0312] 1. Blend the Al’ s Fluopyram, Azoxystrobin and Captan with inert fillers china clay, silica powder and dispersing agents Sodium Isopropyl Naphthalene Sulfonate, Sodium salt of naphthalene sulfonate condensate to aid in dispersion and wetting.

[0313] 2. Grind the mixture to reduce the particle size, aiming for fine particles (10-50 microns) for better dispersion.

[0314] 3. Added Sodium ligno sulphonate to improve the powder's ability to disperse in water.

[0315] 4. If needed, granulate the powder to improve flow and handling.

[0316] 5. Removed the moisture to prevent clumping and ensure stability.

[0317] 6. Finally Sieved the powder to achieve uniform particle size. STABILITY DATA:_Fluopyram 7.2% + Azoxystrobin 6.2 % + Captan 41.6% WP

[0318] Example 9: Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% WG

[0319] CHEMICAL COMPOSITION:

[0320] PREPARATION PROCESS:

[0321] A. Formulation process

[0322] 1. Took china clay in the ribbon blender and started mixing. To this added silica powder, Sodium alkyl naphthalene sulfonate formaldehyde condensate, Sodium lauryl sulphate, sodium lignosulphate, Polymeric dispersant, lactose monohydrate, Fluopyram technical, Trifloxystrobin technical and Cyazofamid technical and mixed well.

[0323] 2. Continued to mix this till the material is completely homogenised.

[0324] B. Grinding process

[0325] 1. Allowed to pass the premix through the Airjet mill at 6 to 7 kg / cm2of grinding pressure and 100 to 150 kg / hr feeding rate so as get a particle size of D90 less than 10 micron. The grinded material was collected in post blender.

[0326] 2. WP was then checked for Al content and suspensibility parameter.

[0327] 3. After approval from QC the process was taken further for extrusion and drying.

[0328] C. Extrusion process

[0329] 1. Into the post blender the solution of water and Polydimethylsiloxane was added by means of spraying and dough was prepared. This dough was mixed well to get a well hydrated dough for extrusion. 2. The dough was then allowed to pass through the screw valve fitted with post blender and then passed through basket extruder (Sieve size of 0.8 mm / 1.0 mm).

[0330] D. Drying Process 1. These wet extruded granules were then passed through VFBG (vibro fluid bed drier) which was brought to a consistent temperature of 50°C prior to drying of wet granules.

[0331] 2. These granules were checked for moisture content which should not more than 3%.

[0332] 3. Once the parameter got set then the process is continued. E. Sieving and packing

[0333] The dried granule was passed through vibro siever machine to separate any lumps or dust and desired size granules are collected and packed.

[0334] STABILITY DATA: Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% WG

[0335] Example 10: Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC

[0336] CHEMICAL COMPOSITION: FORMULATION PROCESS:

[0337] 1. The mixture of surfactants (Acrylic graft copolymer, Sodium Alkylnaphthalene Sulfonate Formaldehyde Condensate, Amine Salt Of a Phosphated Tristyryl Phenol Ethoxylate), Poly dimethylsiloxane emulsion & Propylene Glycol (PG) are first diluted in required D.M.Water, and solubilized by high shear mixin.

[0338] 2. Then to the above, added Fluopyram Technical, Trifloxystrobin Technical, and Cyazofamid Technical mix and made the homogeneous mass.

[0339] 3. The above mixed mass was grinded in bead mill. Grinding was carried out until a mean particle size of below 5 microns was obtained.

[0340] 4. Prepared 2% gum solution - Took 100 g DM water added 0.2 g of 1,2-Benzisothiazolin- 3 -one, then under slow stirring added 2 g xanthan gum. 5. To the grinded mass, added 2% water solution of xanthan gum under low stirring and mixed till homogeneous solution was achieved.

[0341] 6. Finally checked for quality parameter. STABILITY DATA: Fluopyram 16.4% + Trifloxystrobin 7.2 % + Cyazofamid 10.4% SC

[0342] Example 11: Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% WG

[0343] CHEMICAL COMPOSITION: PREPARATION PROCESS:

[0344] A. Formulation process

[0345] 1. Took china clay in the ribbon blender and started mixing. To this added silica powder, Sodiumdiisopropyl naphthlene sulphonate, Sodium ligno sulphonate, Sodium salt of naphthalene sulfonate condensate, Polymeric dispersant, lactose monohydrate, Fluopyram technical, Azoxystrobin technical and Cyazofamid technical and mixed well.

[0346] 2. Continued to mix this till the material is completely homogenised. B. Grinding process

[0347] 1. Allowed to pass the premix through the Airjet mill at 6 to 7 kg / cm2of grinding pressure and 100 to 150 kg / hr feeding rate so as get a particle size of D90 less than 10 micron. The grinded material was collected in post blender.

[0348] 2. WP was then checked for Al content and suspensibility parameter.

[0349] 3. After approval from QC the process was taken further for extrusion and drying.

[0350] C. Extrusion process

[0351] 1. Into the post blender the solution of water and Polydimethylsiloxane was added by means of spraying and dough was prepared. This dough was mixed well to get a well hydrated dough for extrusion.

[0352] 2. The dough was then allowed to pass through the screw valve fitted with post blender and then passed through basket extruder (Sieve size of 0.8 mm / 1.0 mm).

[0353] D. Drying Process

[0354] 1. These wet extruded granules were then passed through VFBG (vibro fluid bed drier) which was brought to a consistent temperature of 50°C prior to drying of wet granules.

[0355] 2. These granules were checked for moisture content which should not more than 3%.

[0356] 3. Once the parameter got set then the process is continued.

[0357] E. Sieving and packing

[0358] The dried granule was passed through vibro siever machine to separate any lumps or dust and desired size granules are collected and packed.

[0359] STABILTY DATA: Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% WG

[0360]

[0361] Example 12: Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC

[0362] CHEMICAL COMPOSITION: FORMULATION PROCESS:

[0363] 1. The mixture of surfactants (Acrylic graft copolymer, Sodium Alkylnaphthalene Sulfonate Formaldehyde Condensate), Polyalkoxy ethers, Polydimethylsiloxane emulsion & Propylene Glycol (PG) are first diluted in required D.M.Water, and solubilized by high shear mixing.

[0364] 2. Then to the above, added Fluopyram Technical, Azoxystrobin Technical, and Cyazofamid Technical mix and made the homogeneous mass.

[0365] 3. The above mixed mass was grinded in bead mill. Grinding was carried out until a mean particle size of below 5 microns was obtained.

[0366] 4. Prepared 2% gum solution - Took 100 g DM water added 0.2 g of 1,2-Benzisothiazolin- 3 -one, then under slow stirring added 2 g xanthan gum.

[0367] 5. To the grinded mass, added 2% water solution of xanthan gum under low stirring and mixed till homogeneous solution was achieved. 6. Finally checked for quality parameter.

[0368] STABILITY DATA: Fluopyram 14.4% + Azoxystrobin 12.4 % + Cyazofamid 9.2% SC

[0369] Evaluation of synergistic effect of fungicidal composition of the present invention

[0370] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0371] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0372] XY E = (X + Y) - -

[0373] 100

[0374] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0375] (XY+YZ+XZ) (XYZ) E = (X + Y + Z)

[0376] 100 10000

[0377] Where:

[0378] E represents expected percentage of fungicidal control for the combination of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0379] X is the percentage of fungicidal control observed by the compound (Fluopyram) at a defined dose (equal to x).

[0380] Y is the percentage of fungicidal control observed by the compound (Trifloxystrobin) at a defined dose (equal to y).

[0381] Z is the percentage of fungicidal control observed by the compound (Difenoconazole) at a defined dose (equal to z).

[0382] When the percentage of fungicidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0383] Observed control (%)

[0384] Ratio = -

[0385] Expected control (%)

[0386] Ratio of O / E > 1, synergism observed

[0387] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0388] Experiment for Synergistic activity of composition of present invention:

[0389] Example 1- Details of experiment on chilli crop:

[0390] Field experiment for synergistic activity of Fluopyram, Trifloxystrobin & Difenoconazole for the control of diseases in chilli was conducted.

[0391] The chilli (Variety: Kashi Anmol) crop was cultivated in 6 m x 5 m plots maintaining 50 cm x 25 cm plant distance with three replications and in Randomized Block Design (RBD). To study the synergistic effect, treatments were applied as foliar spray in the form of SC formulation @ 625 ml / ha. Each active component was applied twice in different ternary and binary compositions. Solo compositions were also taken for comparison. The treatment details are as under:

[0392] The treatments T1 to T28 were used as foliar application whereas no treatment was done for

[0393] T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of disease at experimental plots. The observations for leaf spot disease (causal organism Cercospora capsici) were recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in disease incidence was calculated over control. Based on the per cent reduction at 14 days after second spray in disease incidence, the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 1.

[0394] Table 1: Synergistic effect of composition of the present invention (Fluopyram + Trifloxystrobin + Difenoconazole) against leaf spot incidence in chilli It is clearly evident from the data shown in above Table 1 for per cent leaf spot disease control in chilli that the ternary composition of the present invention comprising Fluopyram, Trifloxystrobin, Difenoconazole i.e., T1 to T4 are synergistic for leaf spot incidence control with > 1 Colby’s Ratio. The binary composition with two fungicides (Fluopyram + Trifloxystrobin, Fluopyram + Difenoconazole and Trifloxystrobin + Difenoconazole) T5 to T 16 are compatible but no synergistic effect was shown for the control of disease. All solo products were also less effective for the control of both insect-pest as compared to ternary composition of the present invention.

[0395] Experiment for Bio-efficacy evaluation of composition of present invention:

[0396] For ternary composition, Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC was used and for binary compositions, Fluopyram 16.4% + Trifloxystrobin 7.2% SC, Fluopyram 16.4% + Difenoconazole 9.6% SC, Trifloxystrobin 7.2% + Difenoconazole 9.6% SC were used. The solo formulations of Fluopyram 16.4% SC, Trifloxystrobin 7.2% SC & Difenoconazole 9.6 % SC were also used for comparison along with control treatment.

[0397] Example 2: Evaluation of the composition of the present invention on chilli

[0398] The synergistic composition of the present invention Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC was evaluated on chilli variety Kashi Anmol. The experiment was conducted in 6 m x 5 m plots maintaining 50 cm x 25 cm plant distance with three replications and in Randomized Block Design (RBD).

[0399] The observations were recorded for the following objectives.

[0400] Objectives:

[0401] 1. Bio-efficacy evaluation against leaf spot disease incidence on chilli

[0402] 2. Bio-efficacy evaluation based on percent reduction in leaf spot disease incidence over control

[0403] 3. Bio-efficacy evaluation based on crop yield.

[0404] Treatment details:

[0405] Methodology:

[0406] The treatments T1 to T18 and T20 were used as foliar application whereas no treatment was done for T19 i.e., control treatment. All treatments were applied at the time of initiation of disease in the experimental plots. The Observations for leaf spot (causal organism Cercospora capsici) was recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and percent disease incidence was calculated. The percent reduction in leaf spot disease was calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on chilli at 1, 3, 7, 10 and 14 days after both applications were also recorded. Results are presented in Table 2 A, 2B and 2C.

[0407] Results:

[0408] Table 2A: Field bio-efficacy evaluation of Fluopyram 16.4% + Trifloxystrobin 7.2% +

[0409] Difenoconazole 9.6% SC against leaf spot disease in chilli figures in parentheses are angular transformed values; NS= Non significant

[0410] Table 2B: Field bio-efficacy evaluation of Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC based on yield of chilli crop.

[0411]

[0412] Table 2C: Phytotoxicity evaluation of Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC on chilli

[0413] From the examples and tables above, it can be clearly seen that the ternary composition of the present invention is superior for controlling the leaf spot disease after 14 days of treatment in chilli crop (Table 2A) as compared to the other binary and solo compositions at the same dosage. The yield of chilli also improved in the ternary composition of Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC as compared to binary and solo compositions (Table 2B).

[0414] The disclosed exemplary composition i.e. Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC @ 500 ml, 625 ml, 800 ml and 1250 ml / ha further showed no phytotoxicity in the chilli crop even after 1, 3, 7, 10 and 14 days after both sprays (Table 2C). It is evident from the above data that the composition of the present invention i.e., Fluopyram 16.4% + Trifloxystrobin 7.2% + Difenoconazole 9.6% SC resulted in good control of leaf spot disease in chilli with higher respective yields as compared to the reference standard products (binary compositions and solo compositions). Hence, the composition of present invention has resulted in synergistic effect and is efficacious. Evaluation of synergistic effect of fungicidal composition of the present invention

[0415] A synergistic effect of two or more products exists whenever the action of an active ingredient composition is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0416] The synergistic action expected for a given composition of two active components can be calculated as follows:

[0417] XY

[0418] E = (X + Y) - -

[0419] 100

[0420] The synergistic action expected for a given composition of three active components can be calculated as follows:

[0421] (XY+YZ+XZ) (XYZ)

[0422] E = (X + Y + Z) - - + -

[0423] 100 10000

[0424] Where:

[0425] E represents expected percentage of fungicidal control for the composition of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0426] X is the percentage of fungicidal control observed by the compound (Fluopyram) at a defined dose (equal to x).

[0427] Y is the percentage of fungicidal control observed by the compound (Azoxy strobin) at a defined dose (equal to y).

[0428] Z is the percentage of fungicidal control observed by the compound (Difenoconazole) at a defined dose (equal to z).

[0429] When the percentage of fungicidal control observed for the composition is greater than the expected percentage, there is a synergism effect.

[0430] Observed control (%)

[0431] Ratio = -

[0432] Expected control (%)

[0433] Ratio of O / E > 1, synergism observed

[0434] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0435] Experiment for Synergistic activity of composition product of present invention:

[0436] Example 3- Details of experiment on Chilli crop: Field experiment for synergistic activity of Fluopyram, Azoxystrobin and Difenoconazole for the control of Anthracnose and Powdery mildew disease on chilli crop was conducted.

[0437] To evaluate the synergistic effect, Chilli (Variety: Kashi Anmol) crop was sown in 5 m x 5 m plots maintaining 45 cm x 45 cm plant distance with three replications and in Randomized Block Design (RBD).The treatments were applied as foliar application at the time of 15 to 20% diseases incidence noticed. Each active component was applied at recommended doses in different ternary and binary compositions. Solo compositions were also taken for comparison. The treatment details are as under:

[0438] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Observations for Anthracnose and Powdery mildew disease were recorded at before spray, 7 and 15 days after application by observing 5 random plants per plot and per cent disease incidence was calculated. The per cent disease incidence was calculated over control. Based on the per cent reduction in Anthracnose and Powdery mildew disease the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 3. Table 3: Synergistic effect of composition product of the present invention (Fluopyram + Azoxystrobin + Difenoconazole) against Anthracnose and Powdery mildew disease in Chilli crop

[0439] It is clearly evident from the data shown in above Table 3 for per cent control Anthracnose and Powdery mildew disease in Chilli crop that the ternary composition of the present invention comprising Fluopyram + Azoxystrobin + Difenoconazole T1 to T4 are synergistic for disease control with > 1 Colby’s Ratio. All binary compositions were observed to show less effective control of both the diseases as compared to ternary compositions. Further, all solo products were also less effective for the control of both insect-pest as compared to ternary composition of the present invention.

[0440] Experiment for Bio-efficacy evaluation of composition of present invention:

[0441] For the ternary and binary composition based on Fluopyram, Azoxystrobin and Difenoconazole evaluation under field conditions, the basic test composition as Fluopyram, Azoxystrobin and Difenoconazole were manufactured specifically by the inventor. For trinary composition, Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG was used and for binary compositions, Fluopyram 14.4 % + Difenoconazole 8 % WG, Fluopyram 14.4 % + Azoxystrobin 12.4 % WG and Azoxystrobin 12.4 % + Difenoconazole 8 % WG and solo formulations of Fluopyram 14.4 % WG, Azoxystrobin 12.4 % WG and Difenoconazole 8 % WG were used. The market samples Fluopyram 34.48% SC, Azoxystrobin 23% SC and Difenoconazole 25 % EC were used for comparison.

[0442] Example 4: Evaluation of the composition product of the present invention on Chilli crop.

[0443] The synergistic composition of the present invention Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG was evaluated on Chilli crop variety Kashi Anmol.The experiment plots are design in Randomized Block Design (RBD) with three replications maintaining a distance of 45 cm x 45 cm between plants and rows.

[0444] The observations were recorded for the following objectives.

[0445] Objectives:

[0446] 1. Bio-efficacy evaluation against Anthracnose disease on Chilli crop

[0447] 2. Bio-efficacy evaluation against Powdery mildew disease on Chilli crop

[0448] 3. Bio-efficacy evaluation based on per cent reduction in Anthracnose disease over control.

[0449] 4. Bio-efficacy evaluation based on percent reduction in Powdery mildew disease over control.

[0450] 5. Bio-efficacy evaluation based on crop yield and percent increase over control.

[0451] Treatment details:

[0452] Methodology:

[0453] The treatments T1 to T18 and T20 were used as foliar application whereas no treatment was done for T19 untreated control plots. The treatments were applied as foliar application at the time of 15 to 20% diseases incidence noticed. Each active component was applied at three doses in different ternary and binary compositions. Solo compositions were also taken for comparison.

[0454] Observations for Anthracnose and Powdery mildew diseases were recorded at Before spray, 7 and 15 days after sprays was recorded on 5 plants per 5 leaves per plant in each replication. The green chilli yield was recorded at different picking as per harvest and phytotoxicity to Chilli crop at 0, 3, 7, 10 & 15 days after spray were also recorded. Results are presented in Table 4 A, 4B, 4C and 4D.

[0455] Results:

[0456] Table 4A: Field bio-efficacy evaluation of Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG against Anthracnose disease of chilli crop

[0457]

[0458] DAS - Days after spray

[0459] Table 4B: Field bio-efficacy evaluation of Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG against Powdery mildew disease of chilli crop.

[0460] DAS - Days after spray Table 4C: Field bio-efficacy evaluation of Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG based on yield of chilli crop. Table 4D: Phytotoxicity evaluation of Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG on Chilli crop.

[0461] From the examples and tables above, it can be clearly seen that the composition of the present invention is superior for controlling the Anthracnose disease at 7 and 15 days after spray (Table 4 A) and for controlling Powdery mildew disease at 7 and 15 days after spray (Table 4B) in Chilli crop as compared to the binary composition and solo compositions at same dosage levels. Other registered products, Fluopyram 34.48% SC, Azoxystrobin 23% SC and Difenoconazole 25 % EC were also evaluated in terms of effectiveness but observed to be less effective as compared to the ternary composition of the present invention. The yield of Chilli crop also improved in the ternary composition of Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG as compared to binary and solo compositions (Table 4C).

[0462] The disclosed exemplary composition i.e. Fluopyram 14.4 % + Azoxystrobin 12.4 % + Difenoconazole 8 % WG @ 562.5 ml, 750 ml and 1500 ml / ha further showed no phyto toxicity in the Chilli crop even after 0, 3, 5, 7, 10 and 15 days after spray (Table 4D).

[0463] Evaluation of synergistic effect of fungicidal composition of the present invention

[0464] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0465] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0466] XY

[0467] E = (X + Y) -

[0468] 100 The synergistic action expected for a given combination of three active components can be calculated as follows:

[0469] (XY+YZ+XZ) (XYZ)

[0470] E = (X + Y + Z) - - + -

[0471] 100 10000

[0472] Where:

[0473] E represents expected percentage of fungicidal control for the combination of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0474] X is the percentage of fungicidal control observed by the compound (Fluopyram) at a defined dose (equal to x).

[0475] Y is the percentage of fungicidal control observed by the compound (Trifloxystrobin) at a defined dose (equal to y).

[0476] Z is the percentage of fungicidal control observed by the compound (Captan) at a defined dose (equal to z).

[0477] When the percentage of fungicidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0478] Observed control (%)

[0479] Ratio = -

[0480] Expected control (%)

[0481] Ratio of O / E > 1, synergism observed

[0482] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0483] Experiment for Synergistic activity of composition of present invention:

[0484] Example 5 - Details of experiment on Chilli crop:

[0485] Field experiment for synergistic activity of Fluopyram, Trifloxystrobin and Captan for the control of Fruit rot and Powdery mildew diseases in chilli crop was conducted.

[0486] To evaluate the synergistic effect, Chilli (Varity: Pusa Jwala) crop was sown in 5 m x 5 m plots at 75 cm x 75 cm plant distance with three replications and in Randomized Block Design (RBD). The treatments were applied as foliar application in the form of WP formulation @ 1500ml / ha. Each active component was applied at recommended dose in different ternary and binary compositions, Solo compositions was also taken for comparison. The treatment details are as under:

[0487] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. All the treatments were applied at the initiation of diseases in the plots.

[0488] Observations for Fruit rot (Collelolrichiim capsici) and Powdery mildew (Leveillula tauricd) were recorded at Before spray, 7 and 15 days after sprays by observed 10 random plants per plot and based on PDI of fruit rot disease, per cent reduction over control was calculated and Powdery mildew per cent disease reduction was also calculated over control. Based on the per cent reduction in fruit rot and powdery mildew PDI the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 5.

[0489] Table 5: Synergistic effect of composition product of the present invention (Fluopyram, Trifloxystrobin and Captan) against Fruit Rot and Powdery mildew disease in Chilli crop.

[0490]

[0491] It is clearly evident from the data shown in above Table 5 for per cent fruit rot control and per cent Powdery mildew control in chilli crop that the ternary composition of the present invention comprising Fluopyram, Trifloxystrobin and Captan T1 to T4 are synergistic for both insect control with > 1 Colby’s Ratio. All the binary compositions and solo composition are were observed to be less effective for control of both diseases as compared to ternary composition of the present invention.

[0492] Experiment for Bio-efficacy evaluation of composition of present invention:

[0493] For ternary composition, Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP was used and for binary compositions, Fluopyram 7.2% + Trifloxystrobin 3% WP, Fluopyram 7.2% + Captan 41.8% WP and Trifloxystrobin 3% + Captan 41.8% WP and solo formulations of Fluopyram 7.2% WP, Trifloxystrobin 3% WP and Captan 41.8% WP were used. The market samples Fluopyram 17.7% w / w + Tebuconazole 17.7%w / w SC, Tebuconazole 50% + Trifloxystrobin 25% WG and Captan 75% WP were used for comparison.

[0494] Example 6 - Evaluation of the composition of the present invention on Chilli

[0495] The synergistic composition of the present invention Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP was evaluated on Chilli crop. The treatments application was done on at initiation of disease in plots sown in Randomized Block Design (RBD) with three replications maintaining a distance of 75 cm x 75 cm between plants and rows.

[0496] The observations were recorded for the following objectives.

[0497] Objectives:

[0498] 1. Bio-efficacy evaluation against Fruit rot on Chilli crop. 2. Bio-efficacy evaluation against Powdery disease on Chilli crop.

[0499] 3. Bio-efficacy evaluation based on per cent reduction in Fruit rot over control.

[0500] 4. Bio-efficacy evaluation based on percent reduction in Powdery disease over control.

[0501] 5. Bio-efficacy evaluation based on green Chilli yield.

[0502] Treatment details:

[0503] Methodology:

[0504] The treatments T1 to T18 and T20 were used as foliar application whereas only water spray was done for T 19 control treatment. The treatments were applied as foliar application at the 5 initiation of diseases. Each active component was applied in different ternary and binary compositions. Solo compositions were also taken for comparison.

[0505] Observations for Fruit rot (Collelolrichiim capsici) and Powdery mildew (Leveillula tauricd) were recorded at Before spray, 7 and 15 days after sprays by observing 10 random plants per plot and based on PDI of fruit rot and reduction over control was calculated and Powdery 10 mildew per cent reduction was also calculated over control. The fruit yield Chilli was recorded at different picking as per harvest. Phytotoxicity to Chilli crop at 0, 3, 7, 10 & 15 days after spray were also recorded. Results are presented in Table 6A, 6B, 6C and 6D.

[0506] Results:

[0507] Table 6A: Field bio-efficacy evaluation of Fluopyram 7.2% + Trifloxystrobin 3% +

[0508] 15 Captan 41.8% WP against Fruit Rot in Chilli crop.

[0509] DAS - Days after spray

[0510] Table 6B: Field bio-efficacy evaluation of Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP against Powdery Mildew in Chilli crop.

[0511]

[0512] DAS - Days after spray

[0513] Table 6C: Field bio-efficacy evaluation of Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP based on fruit yield of Chilli crop.

[0514] Table 6D Phytotoxicity evaluation of Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP on Chilli crop.

[0515]

[0516] From the examples and tables above, it is clearly seen that the exemplary composition of the present invention Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP is superior for Fruit rot at 7 and 15 days after spray (Table 6A) and for controlling powdery mildew disease 5 at 7 and 15 days after spray (Table 6B) in Chilli crop as compared to the binary composition and solo compositions at same dosage levels. Other registered formulated products available in the market, Fluopyram 17.7% w / w+ Tebuconazole 17.7%w / w SC, Tebuconazole 50% + Trifloxystrobin 25% WG and Captan 75% WP were also evaluated in terms of effectiveness and were observed to be less effective as compared to the ternary composition of the present 10 invention. The yield of Chilli crop also improved in the ternary composition of Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP as compared to binary and solo compositions (Table 6C).

[0517] The disclosed exemplary composition i.e. Fluopyram 7.2% + Trifloxystrobin 3% + Captan 41.8% WP @ 1125ml 1500ml, 1875ml and 3000ml / ha further showed no phytotoxicity in the chilli crop even after 0, 3, 5, 7, 10 and 15 days after spray (Table 6D).

[0518] It is evident from the above tables of examples that the composition of the present invention Fluopyram, Trifloxystrobin and Captan resulted in good control in fruit rot and Powdery mildew diseases in Chilli crop with higher respective yields as compared to the reference standard products (binary compositions and solo compositions). Hence, the composition of present invention has resulted in synergistic effect and is efficacious.

[0519] Evaluation of synergistic effect of fungicidal composition of the present invention

[0520] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0521] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0522] XY

[0523] E = (X + Y) - -

[0524] 100

[0525] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0526] (XY+YZ+XZ) (XYZ)

[0527] E = (X + Y + Z) - - + -

[0528] 100 10000

[0529] Where:

[0530] E represents expected percentage of fungicidal control for the combination of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0531] X is the percentage of fungicidal control observed by the compound (Fluopyram) at a defined dose (equal to x).

[0532] Y is the percentage of fungicidal control observed by the compound (Azoxy strobin) at a defined dose (equal to y). Z is the percentage of fungicidal control observed by the compound (Captan) at a defined dose (equal to z).

[0533] When the percentage of fungicidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0534] Observed control (%)

[0535] Ratio = -

[0536] Expected control (%)

[0537] Ratio of O / E > 1, synergism observed

[0538] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0539] Experiment for Synergistic activity of composition of present invention:

[0540] Example 7- Details of experiment on grapes crop:

[0541] Field experiment for synergistic activity of Fluopyram, Azoxystrobin & Captan for the control of diseases in grapes was conducted.

[0542] The grapes (Variety: Sonaka) crop was cultivated in 6 m x 5 m plots maintaining 1 m x 1 m plant distance with three replications and in Randomized Block Design (RBD). To study the synergistic effect, treatments were applied as foliar spray in the form of WP formulation @ 1500 ml / ha. Each active component was applied twice in different ternary and binary compositions. Solo compositions were also taken for comparison. The treatment details are as under:

[0543] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of disease at experimental plots. The observations for powdery mildew disease (causal organism Uncinula necator) were recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in disease incidence was calculated over control. Based on the per cent reduction at 14 days after second spray in disease incidence, the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 7.

[0544] Table 7: Synergistic effect of compositio of the present invention (Fluopyram + Azoxystrobin + Captan) against powdery mildew incidence in grapes

[0545] It is clearly evident from the data shown in above Table 1 for per cent powdery mildew disease control in grapes that the ternary composition of the present invention comprising Fluopyram, Azoxystrobin, Captan i.e., T1 to T4 are synergistic for powdery mildew disease control with > 1 Colby’s Ratio. The binary composition of two fungicides (Fluopyram + Azoxystrobin,

[0546] Fluopyram + Captan and Azoxystrobin + Captan) T5 to T16 were compatible but no synergistic effect was shown for the control of disease. All solo products were also less effective for the control of both insect-pest as compared to ternary composition of the present invention.

[0547] Experiment for Bio-efficacy evaluation of composition of present invention:

[0548] For ternary composition, Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP was used and for binary compositions, Fluopyram 7.2% + Azoxystrobin 6.2% WP, Fluopyram 7.2% + Captan 41.6% WP and Azoxystrobin 6.2% + Captan 41.6% WP were used. The solo formulations of Fluopyram 7.2% WP, Azoxystrobin 6.2% WP and Captan 41.6% WP were also used for comparison along with control treatment. Moreover, all these treatments were compared with the available market standards viz., Fluopyram 17.7% w / w + Tebuconazole 17.7%w / w SC, Azoxystrobin 23% SC and Captan 50% WP.

[0549] Example 8: Evaluation of the composition of the present invention on grapes

[0550] The synergistic composition of the present invention Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP was evaluated on grapes variety Sonaka. The experiment was conducted in Randomized Block Design (RBD) with three replications maintaining a distance of 1 m x 1 m between plants and rows.

[0551] The observations were recorded for the following objectives.

[0552] Objectives:

[0553] 1. Bio-efficacy evaluation against powdery mildew disease incidence on grapes

[0554] 2. Bio-efficacy evaluation based on percent reduction in powdery mildew disease incidence over control

[0555] 3. Bio-efficacy evaluation based on crop yield.

[0556] 4. Phytotoxicity evaluation on grapes crop

[0557] Treatment details:

[0558] Methodology:

[0559] The treatments T1 to T18 and T20 were used as foliar application whereas no treatment was done for T19 i.e., control treatment. All treatments were applied at the time of initiation of disease in the experimental plots. The Observations for powdery mildew (causal organism Uncinula necator) was recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in powdery mildew disease was calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on grapes at 1, 3, 7, 10 and 14 days after both applications were also recorded. Results are presented in Table 8A, 8B, and 8C.

[0560] Results: Table 8A: Field bio-efficacy evaluation of Fluopyram 7.2% + Azoxystrobin 6.2% +

[0561] Captan 41.6% WP against powdery mildew disease in grapes

[0562] figures in parentheses are angular transformed values; NS= Non significant

[0563] Table 8B: Field bio-efficacy evaluation of Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP based on yield of grapes.

[0564]

[0565] Table 8C: Phytotoxicity evaluation of Fluopyram 14.4% + Azoxystrobin 12.4% + Captan 9.2% SC on grapes

[0566] From the examples and tables above, it can be clearly seen that the composition of the present invention is superior for controlling the powdery mildew disease after 14 days of treatment in grapes crop (Table 8A) as compared to the other binary and solo compositions at the same dosage. The yield of grapes also improved in the ternary compositions of Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP as compared to binary and solo compositions (Table 8B).

[0567] The disclosed exemplary composition of present invention i.e. Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP @ 1200 ml, 1500 ml, 1875 ml and 3000 ml / ha further showed no phytotoxicity in the grapes crop even after 1, 3, 7, 10 and 14 days after both sprays (Table 8C).

[0568] It is evident from the above data that the composition of the present invention i.e., Fluopyram 7.2% + Azoxystrobin 6.2% + Captan 41.6% WP resulted in good control of powdery mildew in grapes with higher respective yields as compared to the reference standard products (binary compositions, solo products and market standards). Hence, the composition of present invention has resulted in synergistic effect and is efficacious. Evaluation of synergistic effect of fungicidal composition of the present invention

[0569] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0570] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0571] XY

[0572] E = (X + Y) - -

[0573] 100

[0574] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0575] (XY+YZ+XZ) (XYZ)

[0576] E = (X + Y + Z) - - + -

[0577] 100 10000

[0578] Where:

[0579] E represents expected percentage of fungicidal control for the combination of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0580] X is the percentage of fungicidal control observed by the compound (Fluopyram) at a defined dose (equal to x).

[0581] Y is the percentage of fungicidal control observed by the compound (Trifloxystrobin) at a defined dose (equal to y).

[0582] Z is the percentage of fungicidal control observed by the compound (Cyazofamid) at a defined dose (equal to z).

[0583] When the percentage of fungicidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0584] Observed control (%)

[0585] Ratio = -

[0586] Expected control (%)

[0587] Ratio of O / E > 1, synergism observed

[0588] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0589] Experiment for Synergistic activity of composition of present invention: Example 9- Details of experiment on grapes crop:

[0590] Field experiment for synergistic activity of Fluopyram, Trifloxystrobin & Cyazofamid for the control of diseases in grapes was conducted.

[0591] The grapes crop was cultivated in 6 m x 5 m plots maintaining 1 m x 1 m plant distance with three replications and in Randomized Block Design (RBD). To study the synergistic effect, treatments were applied as foliar spray in the form of SC formulation @ 625 ml / ha. Each active component was applied twice in different ternary and binary compositions. Solo compositions were also taken for comparison. The treatment details are as under:

[0592] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of disease at experimental plots. The observations for powdery mildew disease (causal organism Uncinula necator) were recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in disease incidence was calculated over control. Based on the per cent reduction at 14 days after second spray in disease incidence, the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 9.

[0593] Table 9: Synergistic effect of composition of the present invention (Fluopyram + Trifloxystrobin + Cyazofamid) against powdery mildew incidence in grapes

[0594]

[0595] It is clearly evident from the data shown in above Table 9 for percent powdery mildew disease control in grapes that the ternary composition of the present invention comprising Fluopyram, Trifloxystrobin, Cyazofamid i.e., T1 to T4 are synergistic for powdery mildew disease control with > 1 Colby’s Ratio. The binary composition with two fungicides (Fluopyram + Trifloxystrobin, Fluopyram + Cyazofamid and Trifloxystrobin + Cyazofamid) T5 to T16 are compatible but no synergistic effect was shown for the control of disease. All solo products were also less effective for the control of both insect-pest as compared to ternary composition of the present invention. Experiment for Bio-efficacy evaluation of composition of present invention:

[0596] For ternary composition, Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC was used and for binary compositions, Fluopyram 16.4% + Trifloxystrobin 7.2% SC, Fluopyram 16.4% + Cyazofamid 10.4% SC, Trifloxystrobin 7.2% + Cyazofamid 10.4% SC were used. The solo formulations of Fluopyram 16.4% SC, Trifloxystrobin 7.2% SC & Cyazofamid 10.4% SC were also used for comparison along with control treatment. Moreover, all these treatments were compared with the available market standards viz., Fluopyram 17.7% w / w+ Tebuconazole 17.7%w / w SC, Tebuconazole 50% + Trifloxystrobin 25% WG and Cyazofamid 34.5% SC.

[0597] Example 10: Evaluation of the composition of the present invention on grapes The synergistic exemplary composition of the present invention Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC was evaluated on grapes variety Sonaka. The experiment was conducted in Randomized Block Design (RBD) with three replications maintaining a distance of 1 m x 1 m between plants and rows. The observations were recorded for the following objectives.

[0598] Objectives:

[0599] 1. Bio-efficacy evaluation against powdery mildew disease incidence on grapes

[0600] 2. Bio-efficacy evaluation based on percent reduction in powdery mildew disease incidence over control 3. Bio-efficacy evaluation based on crop yield.

[0601] 4. Phytotoxicity evaluation on grapes crop

[0602] Treatment details:

[0603] The treatments T1 to T18 and T20 were used as foliar application whereas no treatment was done for T19 i.e., control treatment. All treatments were applied at the time of initiation of disease in the experimental plots. The Observations for powdery mildew (causal organism Uncinula necator) was recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in powdery mildew disease was calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on grapes at 1, 3, 7, 10 and 14 days after both applications were also recorded. Results are presented in Table 10A, 10B and 10C.

[0604] Results:

[0605] Table 10A: Field bio-efficacy evaluation of Fluopyram 16.4% + Trifloxystrobin 7.2% +

[0606] Cyazofamid 10.4% SC against powdery mildew disease in grapes

[0607] figures in parentheses are angular transformed values; NS= Non significant

[0608] Table 10B: Field bio-efficacy evaluation of Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC based on yield of grapes

[0609]

[0610] Table IOC: Phytotoxicity evaluation of Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC on grapes

[0611] From the examples and tables above, it is clearly seen that the composition of the present invention is superior for controlling the powdery mildew disease after 14 days of treatment in grapes crop (Table 10A) as compared to the other binary and solo compositions at the same dosage . The yield of grapes also improved in the ternary composition of Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC as compared to binary and solo compositions (Table 10B).

[0612] The disclosed exemplary composition of present invention i.e. Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC @ 500 ml, 625 ml, 800 ml and 1250 ml / ha further showed no phytotoxicity in the grapes crop even after 1, 3, 7, 10 and 14 days after both sprays (Table 10C).

[0613] It is evident from the above data that the exemplary composition of the present invention i.e., Fluopyram 16.4% + Trifloxystrobin 7.2% + Cyazofamid 10.4% SC resulted in good control of powdery mildew in grapes with higher respective yields as compared to the reference standard products (binary compositions and solo products / compositions). Hence, the composition of present invention has resulted in synergistic effect and is efficacious. Evaluation of synergistic effect of fungicidal composition of the present invention

[0614] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0615] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0616] XY

[0617] E = (X + Y) - -

[0618] 100

[0619] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0620] (XY+YZ+XZ) (XYZ)

[0621] E = (X + Y + Z) - - + -

[0622] 100 10000

[0623] Where:

[0624] E represents expected percentage of fungicidal control for the combination of the two or three actives ingredients at defined doses (for example equal to x, y and z, respectively).

[0625] X is the percentage of fungicidal control observed by the compound (Fluopyram) at a defined dose (equal to x).

[0626] Y is the percentage of fungicidal control observed by the compound (Azoxy strobin) at a defined dose (equal to y).

[0627] Z is the percentage of fungicidal control observed by the compound (Cyazofamid) at a defined dose (equal to z).

[0628] When the percentage of fungicidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0629] Observed control (%)

[0630] Ratio = -

[0631] Expected control (%)

[0632] Ratio of O / E > 1, synergism observed

[0633] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0634] Experiment for Synergistic activity of composition of present invention: Example 11- Details of experiment on grapes crop:

[0635] Field experiment for synergistic activity of Fluopyram, Azoxystrobin & Cyazofamid for the control of diseases in grapes was conducted.

[0636] The grapes (Variety: Sonaka) crop was cultivated in 6 m x 5 m plots maintaining 1 m x 1 m plant distance with three replications and in Randomized Block Design (RBD). To study the synergistic effect, treatments were applied as foliar spray in the form of SC formulation @ 750 ml / ha. Each active component was applied twice in different ternary and binary compositions. Solo compositions were also taken for comparison. The treatment details are as under:

[0637] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications at 15 days interval were done of all treatments on the appearance of disease at experimental plots. The observations for powdery mildew disease (causal organism Uncinula necator) were recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in disease incidence was calculated over control. Based on the per cent reduction at 14 days after second spray in disease incidence, the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 11.

[0638] Table 11: Synergistic effect of composition of the present invention (Fluopyram + Azoxystrobin + Cyazofamid) against powdery mildew incidence in grapes

[0639]

[0640] It is clearly evident from the data shown in above Table 11 for per cent powdery mildew disease control in grapes that the ternary composition of the present invention comprising Fluopyram, Azoxystrobin, Cyazofamid i.e., T1 to T4 are synergistic for powdery mildew disease control with > 1 Colby’s Ratio. The binary composition with two fungicides (Fluopyram + Azoxystrobin, Fluopyram + Cyazofamid and Azoxystrobin + Cyazofamid) T5 to T16 were compatible but no synergistic effect was observed for the control of disease. All solo products were also less effective for the control of both insect-pest as compared to ternary composition of the present invention.

[0641] Experiment for Bio-efficacy evaluation of composition of present invention:

[0642] For ternary compositions, Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC was used and for binary compositions, Fluopyram 14.4% + Azoxystrobin 12.4% SC, Fluopyram 14.4% + Cyazofamid 9.2% SC, Azoxystrobin 12.4% + Cyazofamid 9.2% SC were used. The solo formulations of Fluopyram 14.4% SC, Azoxystrobin 12.4% SC and Cyazofamid 9.2% SC were also used for comparison along with control treatment. Moreover, all these treatments were compared with the available market standards viz., Fluopyram 17.7% w / w + Tebuconazole 17 ,7%w / w SC, Azoxystrobin 23% SC and Cyazofamid 34.5% SC.

[0643] Example 12: Evaluation of the composition of the present invention on grapes

[0644] The synergistic exemplary composition of the present invention Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC was evaluated on grapes variety Sonaka. The experiment was conducted in Randomized Block Design (RBD) with three replications maintaining a distance of 1 m x 1 m between plants and rows.

[0645] The observations were recorded for the following objectives.

[0646] Objectives:

[0647] 1. Bio-efficacy evaluation against powdery mildew disease incidence on grapes

[0648] 2. Bio-efficacy evaluation based on percent reduction in powdery mildew disease incidence over control

[0649] 3. Bio-efficacy evaluation based on crop yield.

[0650] 4. Phytotoxicity evaluation on grapes crop

[0651] Treatment details:

[0652]

[0653] Methodology:

[0654] The treatments T1 to T18 and T20 were used as foliar application whereas no treatment was done for T19 i.e., control treatment. All treatments were applied at the time of initiation of disease in the experimental plots. The Observations for powdery mildew (causal organism Uncinula necatof) was recorded at before spray and 14 days after 1stand 2ndapplication by observing 30 random leaves per plot and per cent disease incidence was calculated. The per cent reduction in powdery mildew disease was calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on grapes at 1, 3, 7, 10 and 14 days after both applications were also recorded Results are presented in Table 12 A, 12B, and 12C.

[0655] Results:

[0656] Table 12A: Field bio-efficacy evaluation of Fluopyram 14.4% + Azoxystrobin 12.4% +

[0657] Cyazofamid 9.2% SC against powdery mildew disease in grapes

[0658]

[0659] Table 12B: Field bio-efficacy evaluation of Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC based on yield of grapes

[0660]

[0661] Table 12C: Phytotoxicity evaluation of Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC on grapes

[0662] From the examples and tables above, it is clearly seen that the ternary composition of the present invention is superior for controlling the powdery mildew disease after 14 days of treatment in grapes crop (Table 12A) as compared to the other binary and solo compositions at the same dosage. The yield of grapes also improved in the ternary compositions of Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC as compared to binary and solo compositions as well as available market standards (Table 12B). The disclosed exemplary composition of present invention i.e. Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC @ 600 ml, 750 ml, 900 ml and 1500 ml / ha further showed no phytotoxicity in the grapes crop even after 1, 3, 7, 10 and 14 days after both sprays (Table 12C).

[0663] It is evident from the above data that the composition of the present invention i.e., Fluopyram 14.4% + Azoxystrobin 12.4% + Cyazofamid 9.2% SC resulted in good control of powdery mildew in grapes with higher respective yields as compared to the reference standard products (binary compositions and solo products / compositions). Hence, the composition of present invention has resulted in synergistic effect and is efficacious.

[0664] In particular, it has now been found, surprisingly that, for example, the fungicidal activity of the composition of present invention, compared with the fungicidal activity of individual components, is synergistic. In fact, various advantageous properties associated with the compositions according to the invention, include but are not limited to: a broadening of the spectrum of fungicidal activity to other fungi, for example to resistant strains; a reduction in the rate of application of the active ingredients; adequate control of the fungal disease at a rate of application at which the individual compounds are not very effective, advantageous behaviour during formulating and / or upon application, improved stability, improved crop characteristics including crop yields, more developed root system, increase in plant health including height, improved plant vigor, less plant verse (lodging), and other advantages familiar to a person skilled in the art.

[0665] From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.

Claims

I CLAIM:

1. . A stable synergistic fungicidal composition comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;(B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and(C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

2. . The stable synergistic fungicidal composition as claimed in claim 1, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition; and(C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

3. . The stable synergistic fungicidal composition as claimed in claim 1 and 2, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Trifloxystrobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and(C) Difenoconazole or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

4. . The stable synergistic fungicidal composition as claimed in claim 1 and 2, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Azoxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and(C) Difenoconazole or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

5. . The stable synergistic fungicidal composition as claimed in claim 1 and 2, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Trifloxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and(C) Captan or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

6. . The stable synergistic fungicidal composition as claimed in claim 1 and 2, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Azoxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and(C) Captan or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

7. . The stable synergistic fungicidal composition as claimed in claim 1 and 2, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Trifloxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and(C) Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

8. . The stable synergistic fungicidal composition as claimed in claim 1 and 2, comprising:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition;(B) Azoxy strobin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1-95% by weight of the composition and(C) Cyazofamid or their agrochemically acceptable salts, esters or derivatives, in the range of 0.1-95% by weight of the composition.

9. . The stable synergistic fungicidal composition as claimed in claims 1 to 8, which further comprises one or more agrochemically acceptable excipients.

10. . The fungicidal composition as claimed in claim 9, wherein the agrochemically acceptable excipients comprise of surfactant(s), dispersing agent(s), anti-freezing agent(s), emulsifier(s), anti-foaming agent / defoamer(s), wetting agent(s), biocide / antimicrobial / anti- bacterial agent(s), thickening agent / thickener(s), quick coating agent(s) or sticking agents / sticker(s), spreader(s), binder(s), pH-adjusting agent(s), anti-caking agent(s), adjuvant(s), filler(s) / suspension aid(s), colorant(s), carrier(s), buffering agent(s), polymer(s), rheology modifier(s), solvent(s), co-solvent(s) or a combination thereof.

11. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the surfactant(s) and / or dispersing agent(s) is / are selected from the group comprising Polymeric dispersant, polymeric anionic dispersant, polyarylphenyl ether phosphate, tristyrylphenol ethoxylate & craft copolymer, Polymethyl methacrylate -polyethylene glycol graft copolymer, acrylate copolymer, acrylic graft copolymer, non-ionic proprietary surfactant, block copolymer , ethoxylated tristryl phenol Sulphate, alkyl naphthalene formaldehyde condensate , naphthalene sulfonic acid, sodium salt condensate with formaldehyde, alkyl naphthalene sulfonic acid, methyl naphthalene sulfonic acid , polymer with formaldehyde sodium salt, ethoxylated oleyl cetyl alcohol, polyalkelene glycol ether, ethoxylated fatty alcohol, Sodium polymethyl methacrylate, polyacrylate, sodium polyacrylate, sodium lignosulphonate, calcium lignosulphonate, alkylphenol polyoxyethylene ether, Sodium salt of naphthalene sulfonate condensate , sodium salt of alkyl naphthalene sulfonate condensate, alkyl or polyalkyl naphthalene sulphonate or its sodium salt, Sodium alkyl naphthalene sulfonate, Sodium Isopropyl Naphthalene Sulfonate, Sodiumdiisopropyl naphthlene sulphonate, sodium salt of alkyl or polyalkyl naphthalene sulfonate formaldehyde condensate, polyalkyl naphthalene sulphonate, Amine Salt Of a Phosphated Tristyryl Phenol Ethoxylate,polycarboxylate, Polyalkoxy ethers , fatty alcohol polyoxyethylene ether, alcohol polyglycol ether or mixtures thereof and present in the range of 0.1-20% by weight of the composition.

12. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the wetting agent(s) is selected form the group comprising tristyrylphenol ethoxylate non-ionic emulsifier, mixture of non-ionic surfactants, alkoxylated alcohol, block copolymer, alcohol polyglycol ether, alkyl olefin sulphates, dioctyl sulpho succinate, alkyl naphthalene sulphate, polyalkyl naphthalene sulphonate, alkyl phenol ethoxylates, tri-styrenated phenol ethoxylate, EO / PO block co-polymers, sodium lauryl sulphate, sodium ligno sulphates, Ethanol 2,2,2 - nitrilotris with alpha-(2,4,6-tris(l-phenylethyl)phenyl)- omega-hydroxypoly(oxy-l,2- ethanediyl) phosphate, Amine Salt Of a Phosphated Tristyryl Phenol Ethoxylate, Polyethylene Polypropylene glycol mono butyl ether , Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide or mixtures thereof and present in the range of 0.1-8.0 % by weight of the total composition.

13. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the anti-foaming agent / defoamer(s) is / are selected from the group comprising silicon emulsion based anti-foam agents, Siloxane polyalkyleneoxide, Polydimethylsiloxane emulsion, trisiloxane ethoxylates, silicone oil, silicone compound, C10~C20 saturated fat acid compounds or C8~C10 aliphatic alcohols compound, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide modified polydimethylsiloxane and mixtures thereof and present in the range of 0.01-5.0% by weight of the composition.

14. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the anti-freezing agent(s) is selected from the group comprising glycol, propylene glycol, mono ethylene glycol, Glycerol, glycerine, diethylene glycol, sorbitol, urea, mannitol, polyethylene glycol or mixtures thereof and present in the range of 0.1-12 % by weight of the composition.

15. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the antimicrobial / anti-bacterial agent(s) is / are selected from the group comprising 1,2- benzisothiazolin-3-one, formaldehyde, sodium benzoate / Sodium o-phenylphenate, 5-chloro-2- methyl-4-isothiazolin-3-one & 2- methyl-4-isothiazolin-3-one, potassium sorbate or mixtures thereof and present in the range of 0.01-8.0 % by weight of the composition.

16. . The stable synergistic fungicidal composition as claimed in claim 10, wherein rheology modifier or thickening agent / thickener(s) or filler(s) is / are selected from the group comprising polysaccharides, Lactose monohydrate, carboxymethyl cellulose, bentonite clay, china clay, kaolin, aluminium magnesium silicate, hydroxy propyl cellulose, xanthan gum, gum arabic, gaur gum, silica powder, carbomer, chitosan, silica, precipitated silica or mixtures thereof and present in the range of 0.01-15.0 % by weight of the composition.

17. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the solvent(s) is selected from the group comprising heavy aromatic hydrocarbon, N-methyl pyrollidone(NMP), Di-methyl sulfoxide(DMSO), Solvent C-IX, N- alcohol, alkyl amide, vegetable oil, mineral oil, distilled water, aromatic solvents, water or mixtures thereof and present in the range of 0.1-20 % by weight of the composition.

18. . The stable synergistic fungicidal composition as claimed in claim 10, wherein the emulsifier is selected from the group consisting of a nonionic surfactant of ethoxylated fatty acid type, such as ethoxylated fatty acid mono-, di- or triglycerides or ethoxylated dialkyl sulfosuccinate, Tri-styrenated phenol ethoxylate , Emulsifier (Blend of Anionic and Nonionic), blend of Calcium Alkyl benzene sulphonate and Tri-styrenated phenol ethxylate an alkylaryl sulfonate, a polyoxyethylene fatty acid ester, a polyoxyethylene sorbitol fatty acid ester, a polyoxyethylene castor oil and a polyoxyethylene hydrogenated castor oil., ethoxylated fatty alcohol type or a block copolymer comprising one or more alkylene oxide block type or mixtures thereof and present in the range of 0.1-25 % by weight of the composition.

19. . The stable synergistic fungicidal composition as claimed in claims 1-18, wherein the use of said composition provides increased crop yield and / or improved plant health.

20. . The stable synergistic fungicidal composition as claimed in any one of the claims 1-19, wherein the composition is in form of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspensionconcentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed composition of CS and SC (ZC), a mixed composition of CS and SE (ZE) or a mixed composition of CS and EW (ZW).

21. . A kit-of-parts comprising a plurality of components, wherein said plurality of components comprises:(A)Fluopyram or its agrochemically acceptable salts, esters or its derivatives;(B) Strobilurin fungicide selected from Trifloxystrobin or Azoxystrobin or its agrochemically acceptable salts, esters or its derivatives; and(C) Another fungicide selected from Difenoconazole or Captan or Cyazofamid or their agrochemically acceptable salts, esters or derivatives.

22. . A method for controlling a plant disease caused by a plant-pathogens comprising applying to the plant or portion thereof or soil, a fungicidal effective amount of the fungicidal composition as claimed in claims 1-21.

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