Probe device and fully automatic indirect fluorescence immunoassay instrument comprising same

Abstract

A probe device and a fully automatic indirect fluorescence immunoassay instrument comprising same. The probe device is provided with a rack, a plurality of probes, an arm, and a Y-axis movement assembly configured to drive all the probes to disperse, gather or translate equidistantly in a Y-axis direction, wherein the arm is fitted on the rack, the Y-axis movement assembly is fitted on the arm, and the probes and the Y-axis movement assembly are in transmission fit; and 2n+2 probes are provided, n≥0, and n being an integer, and the probes are distributed in a queue in the arm. In the present invention, the plurality of probes can flexibly move, for example, can disperse, gather or translate equidistantly in the Y-axis direction by means of the arm, and the plurality of probes can disperse to different fluorescent slides on the basis of actual situations and can operate at the same time, thereby improving the operating efficiency.

Description

A probe device and fully automatic indirect immunofluorescence analyzer thereof Technical Field

[0001] The present invention relates to the technical field of immunofluorescence analysis equipment, and in particular to a probe device and a full-automatic indirect immunofluorescence analyzer. Background Art

[0002] Fluorescence immunoassay (FIA) is an immunoassay technique that uses fluorescently labeled antibodies or antigens as tracers, combining the specificity of antigen-antibody reactions in immunology with the sensitivity of fluorescence technology. Its principle is similar to that of ELISA, using a fluorescent substance as a labeled probe that binds to a known antibody or antigen to form a fluorescent antibody complex or fluorescent antigen complex. This fluorescent complex is then immobilized on a carrier such as a glass cellulose membrane or polyester membrane as a capture reagent. A nitrocellulose membrane with the paired antibody or antigen embedded at a specific location serves as the detection reagent. The two interconnected membranes serve as the stationary phase, and the analyte serves as the mobile phase. After a period of time, the analyte migrates across the membrane strip through capillary action, forming an immunofluorescent complex specifically reacting with the antigen and antibody at a specific location on the nitrocellulose membrane. The fluorescence intensity of the fluorescent substance is then measured.

[0003] The immunofluorescence analyzer of the prior art requires the use of multiple probes, which can perform the same function or different functions. For example, the different functions can be divided into adding different samples and cleaning, and the probes are controlled by the same robotic arm or by multiple robotic arms respectively. The distance between the probes controlled by the same robotic arm is fixed. This type of probe device has poor flexibility and cannot achieve the effect of probe division of labor. For example, a fully automatic fluorescent immunoassay disclosed in CN209992518U includes a base plate, a sample adding arm module, a sample adding needle module, a tip box, a sample dilution plate, a reagent module, a sample module, a reagent card storage and automatic loading module, and a turntable module. The probes of this patent are all arranged in the sample adding needle module. The distance between the probes in this sample adding needle module is fixed, so it is impossible to move flexibly in the sample adding needle module. If different robotic arms are used to control the probes, the production cost will be greatly increased, and there may also be a risk of collision between different robotic arms.

[0004] Therefore, in view of the shortcomings of the existing technology, it is necessary to provide a probe device and a fully automatic indirect immunofluorescence analyzer to solve the shortcomings of the existing technology. Summary of the Invention

[0005] One of the purposes of the present invention is to overcome the shortcomings of the prior art and provide a probe device that only requires one arm to carry multiple probes for flexible movement along the Y direction, such as dispersion, aggregation, or equidistant translation.

[0006] The above-mentioned purpose of the present invention is achieved through the following technical measures:

[0007] A probe device is provided, which is provided with a frame, multiple probes, an arm and a Y-axis moving component for driving all the probes to disperse, aggregate or translate at equal distances along the Y-axis direction. The arm is assembled on the frame, the Y-axis moving component is assembled on the arm, and the probes are assembled with the Y-axis moving component through a transmission mechanism.

[0008] The number of the probes is 2n+2, wherein n≥0, and n is a positive integer, and the probes are distributed in a queue in the arm body.

[0009] The probes at the two ends of the queue are defined as the first end needle and the second end needle respectively.

[0010] Preferably, the above-mentioned Y-axis moving assembly is provided with a pair of Y-axis motors, a first synchronous belt and a second synchronous belt, the Y-axis motor is assembled on the arm body, the first synchronous belt is meshed and connected with the motor shaft of one of the Y-axis motors, the second synchronous belt is meshed and connected with the motor shaft of the other Y-axis motor, the first end needle is fixedly connected with the first synchronous belt, the second end needle is fixedly connected with the second synchronous belt, and the first end needle is away from the Y-axis motor, and the second end needle is close to the Y-axis motor.

[0011] When n>0, the probes except the first end needle and the second end needle are defined as intermediate needles.

[0012] Preferably, the Y-axis moving assembly is further provided with a tensioning assembly for driving all the intermediate needles to disperse, aggregate or translate at equal distances, and the number of the tensioning assemblies is n.

[0013] The middle needles in the queue are divided into n pairs from front to back, each of the tensioning assemblies is fixedly assembled on a corresponding pair of the middle needles, and the tensioning assemblies are also engaged with the first synchronous belt and the second synchronous belt respectively.

[0014] In each pair of the middle needles, the middle needle closer to the Y-axis motor is defined as a front needle body, and the other middle needle is defined as a rear needle body.

[0015] Preferably, the tensioning assembly is provided with four steering wheels for steering the synchronous belt and two sets of double gears.

[0016] In each pair of the middle needles, two of the steering wheels are rotatably assembled on the front needle body, and the other two steering wheels are rotatably assembled on the rear needle body, and the four steering wheels are symmetrically assembled, and two sets of double gears are rotatably assembled on one of the middle needles.

[0017] Preferably, the double gear is provided with a first gear and a second gear, the first gear and the second gear are stacked, and the number of teeth of the first gear is greater than the number of teeth of the second gear.

[0018] Preferably, the front needle body is adjacent to the corresponding first gear in the double gears, and the rear needle body is adjacent to the corresponding second gear in the double gears.

[0019] The steering wheel located above the pair of middle needles is defined as an upper steering wheel, and the steering wheel located below the pair of middle needles is defined as a lower steering wheel;

[0020] Preferably, the above-mentioned first synchronous belt first abuts against the upper steering wheel of the front needle body, then engages with the first gear of the double gear on the front needle body, then engages with the second gear of the double gear on the rear needle body, and finally abuts against the upper steering wheel of the rear needle body.

[0021] Preferably, the above-mentioned second synchronous belt first abuts against the lower steering wheel of the front needle body, then engages with the second gear of the double gear on the front needle body, then engages with the first gear of the double gear on the rear needle body, and finally abuts against the lower steering wheel of the rear needle body.

[0022] Preferably, the gear ratio of the number of teeth of the first gear to the number of teeth of the second gear is 2:1.

[0023] The probe device of the present invention is also provided with an X-axis moving component for making the probe move alone along the X-axis direction and a Z-axis moving component for driving the arm to move along the Z-axis direction. The Z-axis moving component is assembled on the frame, the X-axis moving component is assembled on the arm, and the probe is assembled with the X-axis moving component in a transmission manner.

[0024] Preferably, the above-mentioned probe is provided with a first fixed block, a second fixed block, a rack and a needle tube, the first fixed block and the second fixed block are fixedly connected, the arm body is located between the first fixed block and the second fixed block, and the second fixed block is slidably assembled on the arm body, the second fixed block is assembled with the Y-axis moving assembly, the first fixed block is assembled with the X-axis moving assembly, the rack passes through the first fixed block, the rack is engaged with the X-axis moving assembly, and the needle tube is fixedly assembled at the end of the rack.

[0025] Preferably, the arm body is provided with two slide rails, and each of the second fixing blocks is slidably connected to the two slide rails respectively.

[0026] Preferably, the above-mentioned X-axis moving components are provided in multiple sets, and the number of the X-axis moving components corresponds one to one to the number of the probes.

[0027] Preferably, the above-mentioned X-axis moving assembly is provided with an X-axis motor, a spline shaft, a spline sleeve, an outer gear ring and an assembly kit, the X-axis motor is fixedly assembled on the arm body, the spline shaft is assembled on the arm body, and the spline shaft is fixedly connected to the rotating shaft of the X-axis motor, the spline sleeve can be slidably mounted on the spline shaft, the outer gear ring is fixedly assembled on the outside of the spline sleeve, the spline sleeve can be rotatably assembled on the assembly kit and the outer gear ring is embedded in the clearance hole of the assembly kit, the spline sleeve and the outer gear ring rotate inside the assembly kit, and the outer gear ring engages with the rack at the clearance hole.

[0028] Preferably, the first fixing block is provided with a plurality of through holes, and the number of the through holes is not less than the number of the probes.

[0029] In each of the first fixed blocks, one of the through holes is fixedly assembled with the assembly member in one set of the X-axis moving components, and the spline shafts in the other X-axis moving components pass through the other through holes.

[0030] Preferably, the above-mentioned Z-axis moving assembly is provided with a Z-axis motor and a transmission belt, and the Z-axis motor and the transmission belt are respectively assembled inside the frame, the Z-axis motor is transmission-connected to the transmission belt, the transmission belt is fixedly connected to the arm body, and the arm body is located outside the frame body.

[0031] The second object of the present invention is to provide a fully automatic indirect immunofluorescence analyzer that overcomes the shortcomings of the prior art. The fully automatic indirect immunofluorescence analyzer only requires one arm to carry multiple probes for flexible movement along the Y direction, such as dispersion, aggregation, or equidistant translation.

[0032] The above-mentioned purpose of the present invention is achieved through the following technical measures:

[0033] Provided is a full-automatic indirect immunofluorescence analyzer, which is provided with the above-mentioned probe device and a body, wherein the probe device is assembled inside the body.

[0034] A probe device and a fully automatic indirect immunofluorescence analyzer of the present invention, wherein the probe device is provided with a frame, a plurality of probes, an arm body and a Y-axis moving assembly for driving all the probes to disperse, aggregate or translate at equal distances along the Y-axis direction, the arm body is assembled on the frame body, the Y-axis moving assembly is assembled on the arm body, and the probes are assembled with the Y-axis moving assembly in a transmission manner; the number of the probes is 2n+2, wherein n≥0, and n is a positive integer, and the probes are distributed in a queue in the arm body. The plurality of probes of the present invention can be flexibly moved in the Y-axis direction by an arm body, that is, dispersed, aggregated or translated at equal distances, so these probes can be dispersed to different fluorescent slides according to actual conditions and work simultaneously, thereby improving work efficiency, and there is no risk of probe collisions between different robotic arms. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The present invention is further described with reference to the accompanying drawings, but the contents in the drawings do not constitute any limitation to the present invention.

[0036] FIG1 is a schematic structural diagram of a probe device according to Example 1.

[0037] FIG. 2 is a schematic diagram of FIG. 1 from another angle.

[0038] FIG3 is a schematic diagram of FIG1 from another angle.

[0039] FIG4 is a schematic diagram of the structure of the probe and the X-axis moving component during assembly.

[0040] FIG5 is a schematic diagram of the structure of the rack, needle tube, spline sleeve, outer gear ring and assembly kit during assembly.

[0041] FIG6 is a schematic diagram of the structure of the probe.

[0042] FIG7 is a schematic structural diagram of the spline sleeve, the outer gear ring and the assembly kit during assembly.

[0043] FIG8 is a schematic structural diagram of the assembly kit.

[0044] FIG9 is a schematic structural diagram of a probe device according to Example 2.

[0045] FIG10 is a schematic diagram of FIG9 from another angle.

[0046] FIG11 is a schematic structural diagram of a pair of second fixing blocks and a tensioning assembly during assembly according to Example 2. FIG.

[0047] Figure 12 is a schematic structural diagram of a pair of second fixing blocks, a tensioning assembly and a synchronous belt during assembly in Example 2.

[0048] FIG13 is a diagram illustrating the movement of the probe along the Y-axis in Example 2.

[0049] FIG14 is a schematic structural diagram of a probe device according to Example 3.

[0050] FIG. 15 is a schematic diagram of FIG. 14 from another angle.

[0051] Figure 16 is a schematic structural diagram of a pair of second fixed blocks, a tensioning assembly and a synchronous belt during assembly in Example 3.

[0052] In Figures 1 to 16, there are provided:

[0053] Probe 100, first fixing block 110, second fixing block 120, rack 130, needle tube 140, through hole 150,

[0054] Frame 200,

[0055] Y-axis moving component 300,

[0056] Y-axis motor 310, first synchronous belt 320, second synchronous belt 330,

[0057] Tensioning assembly 340, steering wheel 341, double gear 342, first gear 3421, second gear 3422,

[0058] X-axis moving assembly 400, X-axis motor 410, spline shaft 420, spline sleeve 430, outer gear ring 440, assembly kit 450,

[0059] Arm body 500 and slide rail 510 . DETAILED DESCRIPTION

[0060] The technical solution of the present invention is further described with reference to the following examples.

[0061] Example 1

[0062] A probe device, as shown in Figures 1 to 8, is provided with a frame 200, multiple probes 100, an arm 500, an X-axis moving assembly 400 for moving the probes 100 individually along the X-axis direction, a Y-axis moving assembly 300 for driving all probes 100 to disperse, aggregate, or translate equidistantly along the Y-axis direction, and a Z-axis moving assembly 600 for driving the arm 500 to move along the Z-axis direction. The arm 500 and the Z-axis moving assembly 600 are respectively assembled to the frame 200, the Y-axis moving assembly 300 is assembled to the arm 500, the probes 100 are assembled to the Y-axis moving assembly 300, the X-axis moving assembly 400 is assembled to the arm 500, and the probes 100 are assembled to the X-axis moving assembly 400. The X-axis moving assembly 400 of the present invention is provided in multiple sets, and the number of X-axis moving assemblies 400 corresponds to the number of probes 100.

[0063] It should be noted that most of the probes in the prior art are moved up and down in the same direction. The function of the X-axis moving component 400 of the present invention is to control each probe 100 to move independently along the X-axis direction. Therefore, the present invention controls the probes 100 to work alternately through each X-axis moving component, that is, all probes 100 do not need to move up and down in the same direction.

[0064] The function of the Y-axis moving component 300 is to enable all probes 100 to be dispersed at equal distances along the Y-axis, aggregated to one end, or moved simultaneously. The distances between these adjacent probes 100 and the distances between other adjacent probes 100 are equal when dispersed and aggregated, that is, when dispersed, the distances between adjacent probes 100 increase from small to large; when aggregated, the distances between adjacent probes 100 are equal, and the distances between adjacent probes 100 decrease from large to small. When translated, the relative distances between probes 100 remain unchanged.

[0065] It should also be emphasized that the sample holes in the currently common fluorescent slides are all equidistant from each other, so the Y-axis moving assembly 300 can disperse all the probes 100 above different sample holes. Compared with the fixed-distance probe device in the prior art, for example, when the distance between the probes is equal to the distance β between the sample hole and two adjacent sample holes, then this probe device can only cover the space between the adjacent through holes in a certain area of ​​the fluorescent slide during one movement. The probe of the present invention can not only perform the above functions, but also adjust the distance between different probes 100 from β to 2β, 3β, 4β, etc. through the Y-axis moving assembly 300. Therefore, the probe device of the present invention has a larger coverage area for the fluorescent slide. In actual use, the probe of the present invention can perform different divisions of labor, and a probe only needs to absorb the same liquid, and also requires a needle changing operation or a needle washing operation. Therefore, the flexibility of the movement of the probe device of the present invention is greatly improved compared with the prior art.

[0066] The function of the Z-axis moving assembly 600 is to enable the arm 500 to drive all probes 100 to move along the Z-axis.

[0067] The number of probes 100 is 2n+2, where n ≥ 0 and is a positive integer. The probes 100 are arranged in a queue within the arm 500; the probes 100 at the two ends of the queue are defined as the first end needle and the second end needle, respectively. It should be noted that n in the present invention can be a positive integer such as 0, 1, 2, or 10, depending on the actual situation. This embodiment is described assuming n is 0, i.e., there are only two probes 100.

[0068] The Y-axis moving assembly 300 is provided with a pair of Y-axis motors 310, a first synchronous belt 320 and a second synchronous belt 330. The Y-axis motor 310 is assembled on the arm body 500. The first synchronous belt 320 is meshed and connected with the motor shaft of one of the Y-axis motors 310, and the second synchronous belt 330 is meshed and connected with the motor shaft of the other Y-axis motor 310. The first end needle is fixedly connected to the first synchronous belt 320, and the second end needle is fixedly connected to the second synchronous belt 330. The first end needle is away from the Y-axis motor 310, and the second end needle is close to the Y-axis motor 310.

[0069] When the number of probes 100 is 2, that is, there are only two probes 100 in the queue, and the probes 100 are the first end needle and the second end needle, then the first end needle and the second end needle are directly dispersed, aggregated or moved equidistantly through the first synchronous belt 320 and the second synchronous belt 330.

[0070] The probe 100 is provided with a first fixed block 110, a second fixed block 120, a rack 130, and a needle tube 140. The first fixed block 110 and the second fixed block 120 are fixedly connected. The arm 500 is located between the first fixed block 110 and the second fixed block 120, and the second fixed block 120 is slidably assembled on the arm 500. The second fixed block 120 is assembled with the Y-axis moving assembly 300, and the first fixed block 110 is assembled with the X-axis moving assembly 400. The rack 130 passes through the first fixed block 110 and meshes with the X-axis moving assembly 400. The needle tube 140 is fixedly assembled to the end of the rack 130. The arm 500 is provided with two slide rails 510, and each second fixed block 120 is slidably connected to the two slide rails 510. Multiple sets of X-axis moving assemblies 400 are provided, and the number of X-axis moving assemblies 400 corresponds to the number of probes 100.

[0071] It should be noted that each set of X-axis moving components 400 of the present invention controls a corresponding probe 100, so all probes 100 move independently in the X-axis direction, and based on the Y-axis moving component 300, all probes 100 can be dispersed, aggregated or translated at equal distances along the Y-axis direction. Because the sample holes in the fluorescent slide are equidistant, all probes 100 can be located above different sample holes through the Y-axis moving component 300, and the probes 100 can be lowered to different sample holes through the X-axis moving component 400, so that different sample holes can be operated. Therefore, the probe 100 device of the present invention can greatly improve work efficiency, and at the same time, each probe 100 of the present invention has high flexibility.

[0072] The X-axis moving assembly 400 is provided with an X-axis motor 410, a spline shaft 420, a spline sleeve 430, an outer gear ring 440, and an assembly kit 450. The X-axis motor 410 is fixedly mounted on the arm body 500. The spline shaft 420 is assembled to the arm body 500 and is fixedly connected to the rotating shaft of the X-axis motor 410. The spline sleeve 430 can be slidably mounted on the spline shaft 420. The outer gear ring 440 is fixedly mounted on the outside of the spline sleeve 430. The spline sleeve 430 can be rotatably mounted on the assembly kit 450 and the outer gear ring 440 is inserted into a clearance hole of the assembly kit 450. The spline sleeve 430 and the outer gear ring 440 rotate inside the assembly kit 450. The outer gear ring 440 engages with the rack 130 at the clearance hole. The first fixed block 110 is provided with a plurality of through holes 150, and the number of through holes 150 is not less than the number of through holes 150.

[0073] In each first fixed block 110 , one through hole 150 is fixedly assembled with the assembly member 450 in one set of the X-axis moving assembly 400 , and the spline shaft 420 in the other X-axis moving assembly 400 passes through the other through holes 150 .

[0074] It should be noted that the spline shaft 420 in one of the X-axis moving groups of the present invention only meshes with the rack 130 of the corresponding probe 100. When the probe 100 needs to move, the X-axis motor 410 only needs to drive the spline shaft 420 to rotate, thereby driving the rack 130 to move, and the probe 100 can move along the X-axis. The other X-axis moving groups only pass through the other through holes 150 provided in the first fixed block 110 of the probe 100 and are not connected to the rack 130. Therefore, even the other spline shafts 420 are not related to the probe 100. Moreover, the spline shaft 420 and the spline sleeve 430 are slidably mounted together. Therefore, even if the probe 100 moves along the Y-axis, the spline sleeve 430 and the probe 100 as a whole move along the spline shaft 420 along the Y-axis.

[0075] The Z-axis moving assembly (not shown in the figure) is provided with a Z-axis motor and a transmission belt, which are respectively assembled inside the frame 200. The Z-axis motor is connected to the transmission belt for transmission, and the transmission belt is fixedly connected to the arm body 500. The arm body 500 is located outside the frame body 200.

[0076] It should be noted that the Z-axis moving assembly of the present invention is located inside the frame 200 and is therefore not shown in the figures. Moving the arm 500 by means of a motor and a transmission belt is a conventional technology, which should be known to those skilled in the art. Moreover, the structure of the Z-axis moving assembly and the connection relationship with the arm are not the focus of the present invention, so they will not be described in detail here.

[0077] The multiple probes 100 of the probe 100 device can be flexibly moved in the Y-axis direction by being dispersed, aggregated or equidistantly translated through an arm 500, so these probes 100 can be dispersed to different fluorescent slides and work simultaneously. In addition, the present invention also realizes flexible movement in the X-axis and Z-axis directions through the X-axis moving component 400 and the Z-axis moving component, which greatly improves the flexibility of the probe and thus improves work efficiency. Moreover, the present invention only requires one arm 500, which has low production cost and different probes 100 are on the same arm 500 without the risk of needle collision.

[0078] Example 2

[0079] A probe device, as shown in Figures 8 to 12, has other features that are the same as those of Example 1 and also has the following technical features: when n>0 and n≤10, all probes 100 except the first end needle and the second end needle are defined as intermediate needles; n in this embodiment is 1, that is, there are specifically 4 probes 100.

[0080] It should be noted that the probes 100 of the present invention have 2n+2 probes. These probes 100 can be divided into adding samples to the fluorescent slide, adding diluent, performing even suction and spit operations on the samples, adding cleaning solution, adding secondary antibodies, adding glycerol, etc., and can also be used for waste liquid in the fluorescent slide, and the specific division of labor can be carried out according to actual conditions.

[0081] The Y-axis moving assembly 300 is also equipped with a tensioning assembly 340 for driving all the intermediate pins to disperse, converge, or translate at equal distances. The number of tensioning assemblies 340 is n. The intermediate pins in the queue are divided into n pairs from front to back. Each tensioning assembly 340 is fixedly mounted on a corresponding pair of intermediate pins. The tensioning assemblies 340 are also meshed with the first and second synchronous belts 320 and 330, respectively. In this embodiment, there is only one pair of intermediate pins.

[0082] The probe 100 except the first end needle and the second end needle is defined as an intermediate needle, that is, this embodiment 2 has two intermediate needles.

[0083] In each pair of middle needles, the middle needle closer to the Y-axis motor 310 is defined as a front needle body, and the other middle needle is defined as a rear needle body.

[0084] The tensioning assembly 340 is equipped with four steering wheels 341 for steering the synchronous belt and two sets of double gears 342. In each pair of middle needles, two steering wheels 341 are rotatably mounted on the front needle body, and the other two steering wheels 341 are rotatably mounted on the rear needle body. The four steering wheels 341 are symmetrically mounted, and the two sets of double gears 342 are rotatably mounted on each middle needle. The double gears 342 are configured as a first gear 3421 and a second gear 3422. The first gear 3421 and the second gear 3422 are arranged in a stacked manner, and the first gear 3421 has more teeth than the second gear 3422. The front needle body is adjacent to the first gear 3421 of the corresponding double gear 342, and the rear needle body is adjacent to the second gear 3422 of the corresponding double gear 342.

[0085] It should be noted that the synchronous belt of the present invention is directly connected only to the first end needle and the second end needle, and has no direct connection to the middle needle. The function of the double gear 342 is to drive the middle needle to move.

[0086] The steering wheel 341 located above the pair of middle needles is defined as an upper steering wheel 341 , and the steering wheel 341 located below the pair of middle needles is defined as a lower steering wheel 341 .

[0087] The first synchronous belt 320 first abuts against the upper steering wheel 341 of the front needle body, then meshes with the first gear 3421 of the double gear 342 on the front needle body, then meshes with the second gear 3422 of the double gear 342 on the rear needle body, and finally abuts against the upper steering wheel 341 of the rear needle body. The second synchronous belt 330 first abuts against the lower steering wheel 341 of the front needle body, then meshes with the second gear 3422 of the double gear 342 on the front needle body, then meshes with the first gear 3421 of the double gear 342 on the rear needle body, and finally abuts against the lower steering wheel 341 of the rear needle body. The gear ratio of the number of teeth of the first gear 3421 to the number of teeth of the second gear 3422 of the present invention is 2:1. The number of subdivisions of the Y-axis motor 310 is controlled to be 3500 to 4200, which can be determined according to actual conditions.

[0088] It should be noted that the present invention, through the design of the ratio between the number of teeth of the tensioning assembly 340 and the first gear 3421, the number of teeth of the second gear 3422, and the pulse ratio of the Y-axis motor 310, can cause the first end needle, the second end needle, and the middle needle to disperse, converge, or perform equidistant translational motion along the Y-axis. Specifically, when dispersing or converging along the Y-axis, only one Y-axis motor 310 is driven, while the other Y-axis motor 310 is turned off. When all probes 100 are equidistantly translated, both Y-axis motors 310 are driven with the same pulse.

[0089] Since the sample holes on current fluorescent slides are all equidistant from each other, the Y-axis moving assembly 300 of the present invention can simply disperse the first end needle, the second end needle and N pairs of middle needles to different sample holes. At the same time, combined with the X-axis moving assembly 400 and the Z-axis moving assembly 600, different probes 100 can all perform liquid aspiration work at the same time. Moreover, the Y-axis moving assembly 300 can also control the first end needle, the second end needle and N pairs of middle needles to translate at equal distances.

[0090] Compared with the fixed-distance probe device in the prior art, for example, when the distance between the probes is equal to the distance β between the sample hole and two adjacent sample holes, then this probe device can only cover the adjacent through holes in a certain area of ​​the fluorescent slide during one movement. The probe of the present invention can not only perform the above functions, but also adjust the spacing between different probes 100 through the Y-axis moving component 300, from β to 2β, 3β, 4β, etc. Therefore, the probe device of the present invention has a larger coverage area of ​​the fluorescent slide and performs staggered aspiration of the sample holes. In actual use, the probe of the present invention can perform different divisions of labor, such as adding samples, adding diluents, adding cleaning solutions, adding secondary antibodies, adding glycerol, and performing uniform aspiration operations on samples, etc., and a probe only needs to aspirate the same liquid, and also requires needle replacement or needle washing operations.

[0091] Therefore, the flexibility of movement of the probe device of the present invention is greatly improved compared with the prior art.

[0092] When all the probes 100 are translated at equal distances, another batch of sample wells can be aspirated while keeping the distances between the probes 100 unchanged, thereby greatly improving work efficiency.

[0093] This embodiment uses a simplified diagram to illustrate the dispersion, aggregation, or equal-distance translation of all probes 100 along the Y-axis direction. First, the probes 100 of this embodiment are divided into probes 100 1, probes 100 2, probes 100 3, and probes 100 4. In addition, the Y-axis motor 310 is divided into Y-axis motor 310 1 and Y-axis motor 310 2. The specific movement is shown in FIG13 , and the specific process is as follows:

[0094] Step a: The Y-axis motor 310 1 is driven, the Y-axis motor 310 2 is not driven, the probe 100 4 is stationary, the probe 100 3 moves to the left at a speed of α, the probe 100 2 moves to the left at a speed of 2α, and the probe 100 1 moves to the left at a speed of 3α. The probes 100 1, 100 2, and 100 3 are dispersed to the left at equal distances.

[0095] Step b: The Y-axis motor 310 1 and the Y-axis motor 310 2 are driven simultaneously with the same pulse, and the probes 100 1 , 100 2 , 100 3 , and 100 4 move to the left at the same speed, performing equal distance translation;

[0096] Step c: The Y-axis motor 310 1 drives in the reverse direction, the Y-axis motor 310 2 does not drive, the probe 100 4 does not move, the probe 100 3 moves to the right at a speed of α, the probe 100 2 moves to the right at a speed of 2α, and the probe 100 1 moves to the right at a speed of 3α. The probes 100 1, 100 2, and 100 3 converge to the right at an equal distance.

[0097] Step d: The Y-axis motor 310 1 and the Y-axis motor 310 2 are driven simultaneously with the same pulse, and the probes 100 1 , 100 2 , 100 3 , and 100 4 move to the left at the same speed, performing equal distance translation.

[0098] Step e: The Y-axis motor 310 2 is driven in the reverse direction, the Y-axis motor 310 1 is not driven, the probe 100 1 is stationary, the probe 100 2 moves to the right at a speed of α, the probe 100 3 moves to the right at a speed of 2α, and the probe 100 4 moves to the right at a speed of 3α. The probes 100 1, 100 2, and 100 3 are dispersed to the right at equal distances.

[0099] Step f: The Y-axis motor 310 1 and the Y-axis motor 310 2 are driven in opposite directions at the same pulse, and the probes 100 1 , 100 2 , 100 3 , and 100 4 move to the right at the same speed, performing equal distance translation.

[0100] Step g: The Y-axis motor 310 1 is driven in the reverse direction, the Y-axis motor 310 2 is not driven, the probe 100 4 is stationary, the probe 100 3 moves to the right at a speed of α, the probe 100 2 moves to the right at a speed of 2α, the probe 100 1 moves to the right at a speed of 3α, and the probes 100 1, 100 2 and 100 3 converge to the right at an equal distance.

[0101] Compared with Example 1, the number of probes 100 in this embodiment is doubled, and the middle needles can be dispersed, aggregated or translated at equal distances along the Y-axis direction only through a set of tensioning components 340. Therefore, this embodiment does not need to add too many devices while increasing the number of probes 100.

[0102] Example 3

[0103] A probe device, as shown in Figures 14 to 16, has other features that are the same as those of Example 2 and also has the following technical features: n in this embodiment is 2, that is, there are specifically 6 probes 100, including 4 middle needles, and this embodiment has 2 sets of tensioning assemblies 340.

[0104] In this embodiment, two sets of tensioning assemblies 340 are used to drive two pairs of middle needles to disperse, converge or translate at equal distances along the Y-axis. The movement mode of this embodiment is the same as that of embodiment 2, and will not be described in detail here.

[0105] Compared with Example 1, the number of probes 100 in this embodiment is twice as much, and the dispersion, aggregation or equidistant translation of the middle needle along the Y-axis direction can be achieved by only two sets of tensioning components 340. Therefore, this embodiment does not need to add too many devices while increasing the number of probes 100.

[0106] Example 4

[0107] A fully automatic indirect immunofluorescence analyzer is provided with a probe 100 device as described in any one of Examples 1 to 3 and a body, wherein the probe 100 device is assembled inside the body.

[0108] The multiple probes 100 of the fully automatic indirect immunofluorescence analyzer can be flexibly moved in the X, Y, and Z axis directions through an arm 500, thereby reducing production costs while improving work efficiency, and there is no risk of collision between different probes 100.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A probe device, characterized in that: A frame, a plurality of probes, an arm, and a Y-axis moving assembly for driving all the probes to disperse, aggregate, or translate at equal distances along the Y-axis direction are provided, the arm being assembled on the frame, the Y-axis moving assembly being assembled on the arm, and the probes being assembled with the Y-axis moving assembly in a transmission manner; The number of the probes is 2n+2, wherein n≥0, and n is a positive integer, and the probes are distributed in a queue in the arm body.

2. The probe device according to claim 1, wherein: The probes located at the two ends of the queue are defined as the first end needle and the second end needle respectively; The Y-axis moving assembly is provided with a pair of Y-axis motors, a first synchronous belt and a second synchronous belt. The Y-axis motor is assembled on the arm body. The first synchronous belt is engaged with the motor shaft of one of the Y-axis motors, and the second synchronous belt is engaged with the motor shaft of the other Y-axis motor. The first end needle is fixedly connected to the first synchronous belt, and the second end needle is fixedly connected to the second synchronous belt. The first end needle is away from the Y-axis motor, and the second end needle is close to the Y-axis motor.

3. The probe device according to claim 2, characterized in that: When n>0, the probes except the first end needle and the second end needle are defined as intermediate needles; The Y-axis moving assembly is further provided with a tensioning assembly for driving all the intermediate needles to disperse, aggregate or translate at equal distances, and the number of the tensioning assemblies is n; The middle needles in the queue are divided into n pairs from front to back, each of the tensioning assemblies is fixedly assembled on a corresponding pair of the middle needles, and the tensioning assemblies are also engaged with the first synchronous belt and the second synchronous belt respectively.

4. The probe device according to claim 3, characterized in that: In each pair of the middle needles, the middle needle closer to the Y-axis motor is defined as a front needle body, and the other middle needle is defined as a rear needle body; The tensioning assembly is provided with four steering wheels for steering the synchronous belt and two sets of double gears; In each pair of the middle needles, two of the steering wheels are rotatably assembled on the front needle body, and the other two steering wheels are rotatably assembled on the rear needle body, and the four steering wheels are symmetrically assembled, and two sets of double gears are rotatably assembled on one of the middle needles.

5. The probe device according to claim 4, characterized in that: The double gear is provided with a first gear and a second gear, the first gear and the second gear are stacked, and the number of teeth of the first gear is greater than the number of teeth of the second gear; The front needle body is adjacent to the first gear of the corresponding double gear, and the rear needle body is adjacent to the second gear of the corresponding double gear; The steering wheel located above the pair of middle needles is defined as an upper steering wheel, and the steering wheel located below the pair of middle needles is defined as a lower steering wheel; The first synchronous belt first abuts against the upper steering wheel of the front needle body, then meshes with the first gear of the double gear on the front needle body, then meshes with the second gear of the double gear on the rear needle body, and finally abuts against the upper steering wheel of the rear needle body; The second synchronous belt first abuts against the lower steering wheel of the front needle body, then meshes with the second gear of the double gear on the front needle body, then meshes with the first gear of the double gear on the rear needle body, and finally abuts against the lower steering wheel of the rear needle body; The gear ratio of the number of teeth of the first gear to the number of teeth of the second gear is 2:

1.

6. The probe device according to any one of claims 1 to 5, characterized in that: An X-axis moving assembly is also provided for moving the probe alone along the X-axis direction, and a Z-axis moving assembly is provided for driving the arm body to move along the Z-axis direction. The Z-axis moving assembly is assembled on the frame body, the X-axis moving assembly is assembled on the arm body, and the probe is assembled with the X-axis moving assembly.

7. The probe device according to claim 6, characterized in that: The probe is provided with a first fixed block, a second fixed block, a rack and a needle tube, the first fixed block and the second fixed block are fixedly connected, the arm body is located between the first fixed block and the second fixed block, and the second fixed block is slidably assembled on the arm body, the second fixed block is assembled with the Y-axis moving assembly, the first fixed block is assembled with the X-axis moving assembly, the rack passes through the first fixed block, the rack is engaged with the X-axis moving assembly, and the needle tube is fixedly assembled at the end of the rack; The arm body is provided with two slide rails, and each of the second fixing blocks is slidably connected to the two slide rails respectively.

8. The probe device according to claim 7, characterized in that: The X-axis moving components are provided with multiple sets, and the number of the X-axis moving components corresponds one to one to the number of the probes; The X-axis moving assembly is provided with an X-axis motor, a spline shaft, a spline sleeve, an outer gear ring and an assembly kit, the X-axis motor is fixedly assembled on the arm body, the spline shaft is assembled on the arm body, and the spline shaft is fixedly connected to the rotating shaft of the X-axis motor, the spline sleeve is slidably assembled on the spline shaft, the outer gear ring is fixedly assembled on the outside of the spline sleeve, the spline sleeve is rotatably assembled on the assembly kit, and the outer gear ring is embedded in the clearance hole of the assembly kit, the spline sleeve and the outer gear ring rotate inside the assembly kit, and the outer gear ring is engaged with the rack at the clearance hole; The first fixing block is provided with a plurality of through holes, and the number of the through holes is not less than the number of the probes; In each of the first fixed blocks, one of the through holes is fixedly assembled with the assembly member in one set of the X-axis moving components, and the spline shafts in the other X-axis moving components pass through the other through holes.

9. The probe device according to any one of claims 1 to 5, characterized in that: The Z-axis moving assembly is provided with a Z-axis motor and a transmission belt, and the Z-axis motor and the transmission belt are respectively assembled inside the frame. The Z-axis motor is transmission-connected to the transmission belt, and the transmission belt is fixedly connected to the arm body, and the arm body is located outside the frame.

10. A fully automatic indirect immunofluorescence analyzer, characterized in that: A probe device according to any one of claims 1 to 9 and a body are provided, wherein the probe device is assembled inside the body.

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