Method for producing hyperoxygenated fatty acids for medicinal use; medicinal formulations for preventing and treating wounds, and method for producing said formulations containing said acids

Abstract

The present invention relates to a method for producing hyperoxygenated fatty acids (hereinafter HOFA), the method using an O2 concentrator with an atmospheric air flow rate entered of between 1 to 5 litres / minute, at room temperature and pressure, which is the main subject matter of the invention. The first subject matter of the invention, which is dependent on the main subject matter, are medicinal topical formulations using said HOFA as a carrier, and the second secondary subject matter is the method for producing topical agents for healing and / or preventing epidermis damage.

Description

PROCEDURE FOR OBTAINING HYPEROXYGENATED FATTY ACIDS FOR MEDICAL USE; MEDICINAL FORMULATIONS FOR THE PREVENTION AND TREATMENT OF WOUNDS, AND PROCEDURE FOR OBTAINING SUCH FORMULATIONS CONTAINING SAID ACIDS. Field of application of the present invention:

[0001] The present invention finds its field of application in a process for obtaining hyperoxygenated fatty acids (hereinafter AGHO), this being its main objective, and as a first subordinate objective to the main one the topical medicinal formulations that use said AGHO as a vehicle, the second secondary objective being the process for obtaining curative and / or preventive topicals for alterations of the epidermis.

[0002] A "topical formulation" is a medicinal or pharmaceutical preparation that is applied to the surface of the body, including the skin or the inside of the mouth. That is, it is for external use.

[0003] By "medicinal" we mean that the present invention has application both in the treatment of wounds or injuries in humans, and in veterinary medicine, i.e., in the treatment of wounds and injuries in animals. State of the art prior to the present invention:

[0004] Since ancient times, body oils have been used for cosmetic and therapeutic purposes. These oils have compositions rich in Essential Fatty Acids (EFAs). EFAs are polyunsaturated fatty acids. They are defined by the number of carbon atoms and by the position of the double bond in relation to the terminal methyl group (at the opposite end of the chain, in relation to the carboxylic group), providing hydration and restructuring of the epidermis, keeping it in optimal conditions.

[0005] As a non-limiting example of the invention, one of the most important and well-known EFAs is linoleic acid, responsible for numerous functions in our body for cosmetic purposes. It belongs to the group of omega-6 acids and is found mainly in vegetable oils, with sunflower oil having the highest proportion. It is the most abundant polyunsaturated fatty acid in the skin.

[0006] Linoleic acid is one of the ceramides lacking in dry skin. Its deficiency can cause serious disruption of the skin barrier, the visible result of which is a loss of skin elasticity that accelerates aging and can be accompanied by scaling of the epidermis.

[0007] PUFAs are products composed of polyunsaturated essential fatty acids that have undergone a hyperoxygenation process. There are numerous studies on the use of PUFAs in the prevention of pressure sores (PVDs), and all of them attribute multiple properties to them, among which we can highlight: ^ They increase blood microcirculation, reducing the risk of ischemia. ^ They facilitate the renewal of epidermal cells. ^ They enhance cellular cohesion in the epidermis. ^ They increase the skin's resistance to the agents that cause pressure sores. ^ They protect against friction. ^ They reduce skin fragility. ^ They decrease the effect of free radicals. ^ They prevent skin dehydration.

[0008] A vegetable oil is a triglyceride extracted from a plant. The term "vegetable oil" can be narrowly defined to refer only to those vegetable oils that are liquid at room temperature, such as, but not limited to, the following oils: ^ Olive (fruit of the olive tree) ^ Sunflower ^ Soybean ^ Palm (both fruit and seed) ^ Coconut ^ Sesame Peanut Rice Corn Flax (linseed oil) Safflower Hemp Rapeseed, especially the canola (Canadian oil low acid) variety native to Canada, which is suitable for human consumption. ^ almond ^ nut ^ hazelnut ^ grape seeds ^Most of the molecules that make up vegetable oils are lipids, mainly triglycerides (also called triacylglycerols), which are the result of the combination of the three alcohol groups of glycerol (glycerin) with three identical or different fatty acids. They are made up of triglycerides, which are esters of a glycerin molecule with three fatty acids. Most triglycerides are mixed; that is, two or three of their fatty acids are different.

[0009] From the point of view of their chemical formulation, by way of indicative example, it is noted that said oils may have a range of C16 to C18; O16 to O13 and H16 to H20, without this constituting any limitation to the invention.

[0010] Currently, several atomizer containers capable of dispensing AGHO with topical formulations are marketed. Among others, and as an example, PARAFARMIC markets AGHO under its brands NovaMed, Corpitol, Linovera, and Mepentol. BIMEDICA markets Hyperoxygenated Fatty Acids with Novamed® Skincare silicone. ALFASIGMA ESPAÑA, SL sells its AGHO under the brand MEPENTOL AGHO.

[0011] Although OFAs are already known for cosmetic use, all of them base their activity on the release of O2 on the affected areas, with the concentration of oxygen being essential. In the OFAs known in the art, this concentration is around 5 g / liter of oxygen, i.e., low. There is little or no information regarding these O2 concentrations, and the greatest use of OFAs is, as previously stated, for cosmetic purposes, as taught by patent EP 2722050, which covers an oxygenated olive oil for the treatment of ischemia; patent EP 2343044 declares a single cosmetic purpose, as does patent WO / 2010 / 037882.

[0012] Patent EP 1051979 or its counterpart US 6,416,767 granted to Laboratorio CARILENE (FR) teaches the use of a quantity of oxygen incorporated into vegetable oil to convert it into AGHO but which is intended to considerably reduce the irritating character of an active ingredient incorporated in the formulation. Therefore, this precedent US 6,416,767 relates to a novel use of peroxygenated lipids as agents intended to prevent and / or treat the irritating effects of an active ingredient contained in a topical formulation.

[0013] Consequently, as we shall see later, this background radically deviates from what is proposed by the present invention, since this patent US 6,416,767 refers to new compositions for topical application to the skin, which contain an active pharmaceutical ingredient, with irritant character, such as capsaicin and retinoic acid in a cosmetically acceptable vehicle. Drawbacks found in the prior art:

[0014] As already indicated, the properties of first-generation AGHO are mainly cosmetic, and as mentioned above, with reduced concentrations of O2, released from H2O2 and estimated at 5% O2 / liter of air, it is marketed as a topical treatment for wounds and prevention of skin ischemia.

[0015] The only known case in which a therapeutic formulation is offered that inhibits the aggressive action of capsaicin and retinoic acid is given by US patent 6,416,767, so this background does not offer a synergistic action given by a high concentration of O2 released from the peroxide in conjunction with a curative topical preparation.

[0016] Another important factor is that the joint and synergistic action of a second-generation AGHO, i.e. with a high oxygen content, in conjunction with a phyto-therapeutic extract is not known in the prior art.

[0017] Another important factor is the fact that known formulations are usually supplied with sprayers that operate using a small trigger-activated propellant pump. This method of delivery provides low-impact macro-droplets on the epidermis while allowing air to enter the container, as this is the means these systems have to extract the product from the container's interior. If any component present therein is susceptible to oxidation, the product's shelf life is limited. Objectives of the present invention:

[0018] One of the objectives of the present invention is a process for obtaining second-generation AGHO, of medicinal and therapeutic grade, with a higher concentration of O2.

[0019] The secondary objective of the present invention is the topical pharmaceutical or medicinal preparations using AGHO obtained with a high concentration of O 2, developing a synergistic activity with a phytotherapeutic active ingredient, that is, of strict plant and natural origin, with the AGHO and the active ingredient acting together, assisting the action of the active ingredient in support of the regenerative action of the AGHO tissues, and vice versa.

[0020] The objective of the invention is the topical pharmaceutical or medicinal preparations using AGHO obtained with a high concentration of O2, in combination with a collagen suspension with Ag nanoparticles. + , Zn 2+ and Cu + .

[0021] The objective of the invention is that the AGHO with a high concentration of O2 constitutes the vehicle through which the phytotherapeutic active ingredient is applied, or collagen suspension with the aforementioned nanoparticles, the AGHO acting as a means or vehicle for its penetration into the epidermis, dermis, muscle, affected or under treatment.

[0022] Another secondary objective of the invention is the process for obtaining synergistic healing compositions containing the active ingredient and collagen suspensions.

[0023] The objective of the invention is that the AGHO acts in conjunction with the phytotherapeutic product extract, or with the aforementioned collagen, with a total absence of any chemical synthesis molecule, whether of organic or inorganic origin.

[0024] Another objective of the invention is to provide the phytomedicinal preparations thus obtained through a hermetically sealed atomizer or aerosol, using a propellant inert to the oxidation of the active ingredient and with the absence of air, capable of delivering a curtain of micro-droplets onto the tissues, with a relatively strong impact on them due to the pressure of the propellant. Summary of the invention:

[0025] PROCEDURE FOR OBTAINING HYPEROXYGENATED FATTY ACIDS FOR MEDICINAL USE, which includes as a first stage the use of an O2 concentrator, through which entering a flow of atmospheric air between 1 to 5 liters / minute, at room temperature and pressure, a equal volume of N2 free air with an O2 concentration between 90% and 98%; this O2 enriched air stream is sent through a UV emitter, from which it exits with a quantity of 10 to 17 gr. of O3 / liter of air, characterized in that the output of said UV emitter consisting of air + O2 + O3, with the aforementioned quantity of 12 to 17 gr. of O3 / liter of air, is entered into a conduit submerged within a container with a vegetable oil, chosen from the mono and polyunsaturated oils with a range of C16 to C18; O16 to O13 and H16 to H20; Said conduit has at one end an emitter of micro-bubbles of enriched air that expands within the mass of vegetable oil, at the same time that said container is subjected to slow cooling for 5 to 8 hours, until reaching a temperature range of -25ºC to -12ºC, achieving the stabilization and retention of O3 within the solid mass of vegetable oil.

[0026] As a secondary objective, MEDICINAL FORMULATIONS FOR PREVENTION and TREATMENT OF WOUNDS, are characterized by having for every 10 liters of AGHO, 150 to 300gr. of an oil solution of the active ingredient extracted from a herb, chosen from Peppermint, Hypericum Perforatum, Rosa Eglanteria, Equesitum Arbense, Cannabis, Carnicera, Barley, linseed, alfalfa, plantain, boldo. Chamomile, bay leaf, Aloe vera, olive, Calendula, Comfrey, Manuka, Saccharum officinarum L, Beta vulgaris, Lavender, Centella asiatica, Mallow, Witch hazel, Arnica, Yarrow, Sage, Thyme, Tea tree, Rosemary, Chickweed, Plantain, Shepherd's purse, Calendula, Ceibo, Oregano, Pennyroyal and other herbs of phytotherapy that can be an active complement to our vehicle for specific treatments and mixtures of two or more of them, with a total concentration of the phytotherapeutic active ingredient between 2% and 5% per liter of oil.

[0027] The secondary objective of the invention is the MEDICINAL FORMULATIONS FOR PREVENTION and TREATMENT OF WOUNDS, characterized by having in an AGHO AGHO, a suspension of 2% to 5% of collagen with which nano-particles of Silver, Zinc or Copper are mixed with a concentration of 100 to 140 ppm

[0028] As a secondary objective, the PROCEDURE FOR OBTAINING SUCH FORMULATIONS THAT CONTAIN SAID ACIDS is characterized because 150 to 300 Gr are immersed in 10 liters of vegetable oil.of one of the aforementioned herbs in a dry state, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and essential oils and capable of retaining solid particles; the oil is subjected to a heating process between 70ºC to 85ºC for 8 to 12 hours, it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped therein, and during the thawing process, at approximately 0ºC, the enriched oil is mixed with the extract of the active ingredient of the selected herb, shaking and stirring for 10 to 15 minutes, to immediately proceed to package the oil in an air-free atmosphere and before further oxidation of the active ingredient of the selected herb occurs, inside an airtight container equipped with a spray nozzle capable of diffusing the composition. forming micro-droplets with a high impact on the tissues, said container having inside a propellant inert to the oxidation of the active ingredient,

[0029] As a further secondary objective, the PROCEDURE FOR OBTAINING SUCH FORMULATIONS THAT CONTAIN SAID ACIDS is characterized in that for each liter of AGHO, 2% to 5% of collagen is suspended, in whose mass a suspension of nano-particles of Silver, Zinc or Copper with a concentration of 100 to 140 ppm has previously been made.

[0030] One of the possible installations or equipment used for obtaining AGHO according to the present invention is illustrated in Figure 1. Detailed description of the preferred construction of the present invention:

[0031] For the purpose of exemplifying the preferred embodiment of the present invention, the following drawing is attached to illustrate it, supported by the description thereof given below. These embodiment examples should be interpreted as one of the many possible constructions of the invention, and therefore no limiting value should be assigned to them, with possible means equivalent to those illustrated being included within the scope of protection of the invention; the scope of the present invention being determined by the first claim attached in the corresponding Claims chapter.

[0032] If we analyze what is known in the art as an "oxygen concentrator," unlike tanks or cylinders that contain a certain amount of compressed oxygen, concentrators use electric pumps to concentrate the oxygen in the air to provide a continuous supply of O2. An oxygen concentrator is a device that concentrates oxygen from a gas stream (usually ambient air) by selectively separating it from nitrogen. These oxygen concentrators use molecular sieves to adsorb gases and operate on the principle of rapid pressure reversal adsorption of atmospheric nitrogen onto zeolite minerals at high pressure. These devices are commercially available under the brands Philips, Yuwell Spark, Inogen, Juren, and Rhytm Healthcare, among others.

[0033] It is also known in the art that it is possible, in an O2 stream, subjected to an external stress, such as a spark, electric discharge or UV emission, for the appearance of O3, which, as is known, is highly unstable and has a short life. For the purposes of the present invention to "trap" or retain the largest amount of O3, the present invention is characterized by achieving rapid solidification by lowering the temperature of the oil until it freezes with the O2-O3 micro-bubbles dispersed and trapped inside.

[0034] The main objective of this invention is a process for obtaining AGHO, which constitutes a second generation of hyperoxygenated fatty acids, while its secondary objectives are a new and innovative product for the prevention and treatment of wounds.

[0035] Unlike first-generation fatty acids, the AGHO obtained through the process of the invention is not a cosmetic product: it is a second-generation, class III medical product, authorized by the authorities of the Republic of Argentina (ANMAT).

[0036] First-generation fatty acids were used for the prevention of pressure ulcers as a preventative product in skin care, cosmetic-type products that could only be used for prevention and care.

[0037] On the contrary, the second-generation fatty acids obtained with the process of the present invention are adjuvant vehicles with a synergistic action with an active ingredient, giving rise to innovative products created to reinforce the action aimed at modulating and regenerating the protective layer of the skin with a reinforcement function in the cellular membrane by the antioxidant action and reducing effect of free O2 given by the vehicle (AGHO) and which therefore acts as an anti-inflammatory and restorer of rejuvenating effect on tissues as well as the oxidation of biofilm. As already mentioned, the same body temperature of the patient in the affected region promotes the gradual release of O2 trapped in the AGHO.

[0038] The sum of these actions generates a synergy for tissue repair and for the maintenance of the physiological conditions of the skin, being able to provide an appreciable improvement in the different skin defects, maintaining the quality of photosensitive skin or skin with chronic damage such as those subjected to prolonged corticosteroid treatments or burned. Second generation fatty acids use the active ingredient of first generation hyperoxygenated fatty acids, incorporating new substances and concentrations, based on new standards and processing methods that, once analyzed and tested in laboratories, allowed through Biological tests and clinical evaluations demonstrate that AGHO are not cytotoxic, irritant, or sensitizing, making them fully biocompatible, thus obtaining their accreditation as a Class III medical product.

[0039] The second-generation AHO fatty acids of the present invention thus comprise a curative formulation, said AHO acting both as vehicles for the active ingredient of a plant species, suitable for the treatment of a specific ailment, while simultaneously releasing O2 and acting as a bactericide and curative agent. That is, according to the compositions of the present invention, the activity of the AHOs thereof is twofold and synergistic with the active ingredients incorporated. These formulations use as a source a combination of oily herbal extracts that provide essential oils not present in the first-generation fatty acids.

[0040] According to the process for obtaining the present invention, the oils are subjected to a complex hyperoxygenation system and cryogenic process for a higher concentration of oxygen in the formulations achieved, (peroxides), which when in contact with the tissue release oxygen on demand and in the face of biofilm release oxygen completely.

[0041] When detailing the procedure for concentrating and capturing O2-O3 in the oil mass, it is of fundamental importance for the purposes of the same that, taking into account the short and unstable useful life of O3, the cryogenization effect is as fast as possible.

[0042] With the help of Figure 1, the process of concentrating O2-O3 in the oil mass is described in detail, giving rise to the second generation AGHO. In said figure, the generic reference (10) is indicated to the oxygen concentrating device, known in the art, which consists of an air inlet (11) at atmospheric pressure and room temperature with a flow rate between 1 to 5 liters / m. From the inlet (11) through a conduit, the air flow is driven by a pump (12) which feeds a heat exchanger (13) from which, through the conduit (14), it feeds at least one column of zeolite bed (15) in which the N2 is absorbed or separated from the O2 contained in the air flow. At the exit of (15) through the conduit (16) it enters a product tank (17) (air without N2) from which and through the conduit (18) it enters a pressure regulator (19), in series with a flowmeter (20).

[0043] At the outlet of the flow regulator, the air already enriched with O2 is sent through the duct (21) and in a known manner to a UV emitting lamp installation, (22) which causes a discharge of O3, with a concentration at the outlet (23) of O 2 -EITHER 3 10 to 17 gr. of O 3 / liter of air, with a possible insertion in (23) of a cooling device.

[0044] The process of the invention is characterized in that the output of said UV emitter consisting of air + O2 + O3, is entered into a conduit (31), preferably an elbowed conduit, submerged within a container (32) with a vegetable oil (36) chosen from among the mono and polyunsaturated oils with a range of C16 to C18; O16 to O13 and H16 to H20; then the elbow (33) said conduit (31) has at its free end an emitter (34) of micro-bubbles of air. enriched with O2-O3 that expand within the mass of vegetable oil (36), at the same time that said container is subjected to rapid cooling with an external contribution from a cold source (37), until reaching a temperature range of -25ºC to -12ºC, achieving the stabilization and retention of O2 within the oil (36) within the solid (frozen) mass of vegetable oil. Conveniently, the container (32) with oil (36) is located within a casing (38) capable of transmitting the cold until the oil (36) freezes.

[0045] The medicinal formulations for the prevention and treatment of wounds of the invention have for every 10 liters of oil 150 to 300gr. of an oil solution of the active ingredient extracted from a herb in its dry state, chosen from Peppermint, Hypericum Perforatum, Rosa Eglanteria, Equesitum Arbense, Cannabis, Carnicera, Barley, linseed, alfalfa, plantain, boldo. Chamomile, bay leaf, Aloe vera, olive, Calendula, Comfrey, Manuka, Saccharum officinarum L, Beta vulgaris, Lavender, Centella asiatica, Mallow, Witch Hazel, Arnica, Yarrow, Sage, Thyme, Tea Tree, Rosemary, Chickweed, Plantain, Shepherd's Purse, Calendula, Ceibo, Oregano, Pennyroyal and mixtures of two or more of them, and 2% to 5% by volume of this solution is mixed with the frozen oil of the AGHO. For example, if we have a 20-liter reactor, the solution with the active ingredient is 0.4 to 1.0 liters.

[0046] As a secondary objective, the PROCEDURE FOR OBTAINING SUCH FORMULATIONS THAT CONTAIN SAID ACIDS is characterized in that 150 to 300 Gr. of one of the aforementioned herbs in a dry state and properly conditioned are immersed in 10 liters of vegetable oil, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process between 70 to 85ºC for 8 to 12 hours, allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC of the AGHO, the enriched oil is mixed with the extract of the active ingredient of the selected herb, shaking and stirring for 10 to 15 minutes, resulting in a concentration of 2 to 5% of the phyto-therapeutic active ingredient / liter of vegetable oil to immediately proceed to package the oil in an airtight container under internal pressure in an airless atmosphere, using a propellant inert to the oxidation of the active ingredient and before further oxidation of the active ingredient of the selected herb occurs.

[0047] The therapeutic or medicinal product or formulation is incorporated into an aerosol container, which, using a propellant gas and in the absence of oxygen, is capable of creating a flow of micro-droplets that are propelled by the pressure generated by the propellant inside the container, allowing the topical composition to reach areas where dermal absorption would otherwise make it impossible to access. This container guarantees the preservation of the formula from the product's production to the end of its contents by being deprived of atmospheric oxygen. That is, oxidation of the active ingredient is prevented and the thermodynamic equilibrium conditions of the fluid are maintained.

[0048] This new line of fatty acids represents a new paradigm, as in addition to providing therapeutic properties at any depth of the injured skin caused by the HOFA itself, the active ingredient contributes to the healing of the dermal injury. It is an important tissue regenerator, thanks to the gradual supply of oxygen to the tissue, resulting from the release of O2 and O3 remnants contained in the HOFA, and by reducing the levels of free radicals that accumulate in poorly irrigated tissue.

[0049] In the presence of bacterial biofilm, the product reacts by completely and rapidly releasing oxygen bubbles, which disrupt the three-dimensional structure of the biofilm, facilitating its removal. Examples:

[0050] Example 1: Empirical measurement of O2-O3 content present in the AGHO. A flow of 5 liters / minute of N2-free air from an O2 concentrator is entered into a UV-emitting lamp. The experiment started at 8:45 a.m., at an initial temperature of 26.7 ° C and 46% relative humidity. From the outlet of the UV lamp, the air stream enters a mass of sunflower oil with a weight of 1,075 gr without free oxygen "trapped" or "retained" in said mass of oil. At 12:30 p.m. on the same day, the frozen mass of oil is removed with the O2-O3 micro-bubbles retained there, and the frozen oil mass is weighed, with a weight of 1,079 gr., that is, with a content of 4 g of O2-O3 / liter of sunflower oil. If we extrapolate this to the 15 l / h of air introduced in the AGHO production example, we find that 60 g of O2 and O3 are retained within the frozen AGHO mass, an unprecedented amount for a known AGHO.

[0051] Trial 1a * A 41-year-old male patient, MT, presented with post-surgical dehiscence. The lesion was treated traditionally with sugar. The patient presented with two comparable wounds. One of them was treated with sugar. Sugar is known to promote bacterial inhibition and actively participate in wound healing mechanisms, encouraging macrophage activation, eliminating purulent secretions and sloughing areas, promoting the formation of microcapillaries, achieving a more nourished and moist wound, and aiding in wound debridement, achieving more physiological healing according to various studies. Its application causes burning and pain in the patient, with healing taking place on average twice a day. The average wound closure time was 70 days, although with repetition of the trial, varying the quantities and time between applications could reduce this time to 60 days.

[0052] Trial 1b * In the same 41-year-old male patient MT with post-surgical dehiscence, the lesion was treated traditionally with honey. After sterilization with gamma rays to prevent botulism, a layer of honey was applied to a second wound of the same patient using a needle. Primary sterile gauze dressing to prevent honey from slipping. Honey was changed every three to four days. Honey helped eliminate infectious microorganisms present in the wound bed and debride necrotic tissue, stimulating the growth of new cells and tissues, which facilitate wound repair and autolytic debridement through osmotic action. However, studies show that topical application is difficult due to its viscosity. The wound closure time was 95 days.

[0053] Trial 1c * The same male patient MT, aged 41 years, with post-surgical dehiscence, treated the lesion with topical preparations containing AGHO of the invention with a Spearmint composition. A third and fourth wound of the same patient were treated, both in magnitude and depth of the lesion comparable with trials 1a and 1b mentioned above, in order to compare the results. Home treatment was initiated using a topical formulation containing AGHO of 2 da .generation of the present invention at 4% Spearmint / liter of oil, applied by spraying the formulation at a distance of 15 cm onto a primary dressing made of water-repellent and cross-linked polyurethane, plus a secondary Clinicare® dressing, with changes every 48 hours at the beginning, moving after 15 days of treatment to a change every 4 days, achieving wound closure in 30 days.

[0054] Trial 2a * Outpatient, NH female, 58 years old, decompensated type 1 diabetic, with several severe lesions on the sole of the foot in the heel region, treated the lesion in a traditional way with sugar. One of the NH wounds was treated with sugar. In patients with diabetic foot (pathological condition associated with the breakdown of the skin barrier by defects vascular and infectious), with chronicity in this type of soft tissue injuries. The use of sugar is due to its antiseptic, antibacterial, bacteriostatic, and healing properties. It achieves a moist environment in the wound bed, promoting angiogenesis. However, studies show that with sugar in these cases, closure is temporary due to intrinsic factors, repeated trauma, infections, advanced age, nutritional status, joint mobility, and muscle strength. In general, healing is delayed (more than 90 days).

[0055] Trial 2b * The same NH patient, female, 58 years of age, decompensated type 1 diabetic, with a severe lesion on the sole of the foot, treated the lesion in a traditional way with honey. As in Trial 2a, for this type of lesions, i.e. diabetic foot (pathological condition associated with the breakdown of the skin barrier by vascular and infectious defects) on a second comparable wound of the same patient is treated with honey applications, which only produces temporary closure of the wound and healing is delayed (more than 90 days).

[0056] Trial 2c * On the same NH patient, outpatient, female, 58 years old, decompensated type 1 diabetic, with a severe lesion on the sole of the foot, treating the lesion with topical preparations containing AGHO of the invention. Treatment is started on a third wound of the same patient, a diabetic foot wound, in a public institution in the Buenos Aires province, treating it with the application of 2nd generation hyperoxygenated fatty acids with a formulation containing 1% Shepherd's Purse, 2% Pennyroyal and 2% Oregano, per liter of oil. The topical formulation is applied on a primary dressing of Water-repellent and cross-linked polyurethane plus secondary dressing, gauze bandage with changes every 72 hours, achieving healing by secondary intention in 60 days.

[0057] Trial 3a * Male patient "1", 54 years old, with diabetic foot and four severe lesions on the sole of the foot. One of the lesions was treated traditionally with sugar. Its use is in patients with diabetic foot (a pathological condition associated with the breakdown of the skin barrier due to vascular and infectious defects), with chronic soft tissue lesions. The use of sugar is due to its antiseptic, antibacterial, bacteriostatic, and healing properties. It achieves a moist environment in the wound bed, promoting angiogenesis. However, studies show that with these methods, closure is temporary due to intrinsic factors, repeated trauma, infections, advanced age, nutritional status, joint mobility, and muscle strength. In general, healing is delayed (more than 90 days).

[0058] Trial 3b * Male patient “1”, 54 years old, with diabetic foot and severe lesion on the sole of the foot. A second lesion of the same patient was treated in a traditional way with honey. Its use is in patients with diabetic foot (a pathological condition associated with the breakdown of the skin barrier due to vascular and infectious defects), with chronicity of soft tissue lesions. The use of honey is due to its antiseptic, antibacterial, bacteriostatic and healing properties. It achieves a moist environment in the wound bed, promoting angiogenesis. Studies show that with these methods, closure is temporary due to intrinsic factors, repeated trauma, infections, advanced age, nutritional status, joint mobility, and muscle strength. Healing is generally delayed (more than 90 days).

[0059] Essay 3c * Male patient “1” aged 54 years with diabetic foot with a third severe lesion on the sole of the foot, treating the lesion with topical preparations containing AGHO of the invention. Treatment is started in a public institution in the Buenos Aires suburbs with a multiple oxygen concentrator and occlusion using 2nd generation hyperoxygenated fatty acids with a formulation containing 3% Aloe vera and 1% Calendula, applying it on a primary dressing of water-repellent and cross-linked polyurethane plus a Clinicare® secondary dressing with changes every 48 hours, achieving healing by second intention in 28 days on average for grafting.

[0060] Trial 4a * A 24-year-old male patient with two grade 3 plastic burns from a work-related accident was treated traditionally with sugar and / or honey. Honey and sugar are not used for this type of injury due to the limited risk of liquefaction due to the anatomical location of the wound, so there is no healing time with honey or sugar.

[0061] Trial 4b * A 24-year-old male patient with a grade 3 plastic burn from a work-related accident treated traditionally with silver sulfadiazine. One of the wounds was treated with a daily dressing change, and the superficial wound was cleaned, followed by a second application of silver sulfadiazine. The wound took 65 days to heal, with tissue regeneration.

[0062] Trial 4c * A 24-year-old male patient with a grade 3 plastic burn due to an occupational accident was treated with topical preparations containing the invention's AGHO. The other wound, comparable to the first, was treated at home with 2-fold hyperoxygenated fatty acids. da generation with a formulation containing 2% Hypercurium Perforatum, 1% Manuka Honey, 1% Lavender and 1% Centella Asiatica, with applications 4 times a day achieving healing by secondary intention in 17 days.

[0063] Trial 5a * Female patient. A 73-year-old HM with three pressure injuries (PIE) in the lumbosacral region treated with traditional methods using sugar. In this type of injury, sugar acts as a debrider, antibacterial, antiseptic, regenerates perilesional vascular tissue, and heals wounds due to its ability to absorb fluids from the wound bed. However, given their characteristics, they require complementary treatments such as proteolytic enzymes such as collagenase, trypsin, and chymotrypsin; healing in a humid environment with hydrogels, alginates, and foam dressings. The application of a silver hydrogel and dressing was continued until signs of infection / colonization disappeared. Antimicrobial hydroactive colloidal gels would also be indicated. Oral antibiotic therapy was administered by a physician due to the presence of cellulitis and subsequently confirmed by antibiogram.In addition, vacuum treatment is used to close the wound. By applying sugar and other ingredients detailed above to one of the wounds, the wound is closed. of the average wound with the combination of any of these therapies associated with the use of sugar in 150 – 250 days, depending on the patient's metabolic response according to different clinical studies presented.

[0064] Trial 5b * Female patient. A 73-year-old HM with a second pressure injury (PPI) in the lumbosacral region was treated traditionally with honey. As in Trial 5, the same healing procedure was performed, but honey was applied to the wound. The wound closure time with a combination of these therapies associated with the use of honey was estimated at 150–250 days, depending on the patient's metabolic response according to the different clinical studies presented.

[0065] Trial 5c * Female patient. 73-year-old MH with a third comparable pressure injury (PPI) in the lumbosacral region, treating this third injury with topical preparations containing AGHO of the invention. Home treatment was initiated with 2nd generation hyperoxygenated fatty acids, with a formulation containing 1% Thyme, 2% Rosemary and 0.5% Llante, per liter of oil. It was applied topically over a primary dressing made of water-repellent and cross-linked polyurethane plus a Clinicare® secondary dressing with changes every 72 hours, achieving healing by second intention in an average of 135 days.

[0066] Trial 6a * A 52-year-old male patient, AG, was diagnosed with multiple anoscrotal fistulas. He received sugar treatment. They did not generate a response in this type of injury due to the limited risk of liquefaction due to the anatomical location of the wound. That is, due to the severity of the wound, sugar could not be applied as a healing treatment.

[0067] Trial 6b * A 52-year-old male patient, AG, was diagnosed with multiple anoscrotal fistulas. Treatment with honey. This type of injury did not respond due to the limited risk of liquefaction due to the anatomical location of the wound. That is, due to the severity of the wound, honey could not be used as a healing treatment.

[0068] Essay 6c * Male patient AG, 52 years of age, diagnosed with multiple anoscrotal fistula, treating this lesion with topical preparations containing AGHO of the invention. Home treatment is initiated with a multiple oxygen chamber, 2nd generation hyperoxygenated fatty acids with a formulation of 2% Aloe vera, 1% Chickweed, 1% Pennyroyal and 2% Olive. It is applied topically over a primary dressing of water-repellent and cross-linked polyurethane with changes every 24 hours, achieving healing by secondary intention in 105 days on average.

[0069] Essay 7c * Female patient. FC, 36 years old, with oncological pathology with a post-surgical diagnosis of right lower limb amputation. She begins home treatment with a multiple oxygen chamber and occlusion using 2nd generation hyperoxygenated fatty acids, with a formulation containing 1% sugarcane, 2% aloe vera and 1% butcher's milk, applied on a primary dressing of water-repellent and cross-linked polyurethane plus dressing Clinicare® secondary with changes every 48 hours achieving complete healing in 120 days.

[0070] Veterinary trial Case 1: PATIENT: A 7-year-old, 7-month-old male dog with a wound in the interdigital region of his left forelimb. Treatment was carried out with AGHO, with application and bandaging every 48 hours for 2 weeks, then switching to daily application without bandaging for another 2 weeks, resulting in complete healing within that time. An advantage observed over traditional treatment with sugar or propolis was the great patient acceptance due to the lack of pain, burning, or itching. This also contributed to the speed of regeneration / epithelialization in a poorly ventilated interdigital area.

[0071] Veterinary Trial Case 2: PATIENT: A 6-year-and-6-month-old male canine with a surgical wound dehiscence on the dorsal metatarsal region of the left hindlimb. Treatment with AGHO was initiated with daily application and bandage coverage. Closure was achieved by secondary intention in 25 days. The observed advantages of this treatment over traditional sugar or propolis treatments were lower humidity, a wound with less residue, a more hygienic wound, easy application and maintenance, and high patient acceptance due to the absence of burning or pain.

[0072] The results of these tests are summarized in the following table, remembering that the lesions for each set of cases are lesions on the same patient, who voluntarily agreed to the aforementioned tests, comparable in magnitude or severity, taking into account in said Table only the healing or scarring time of the wound as a variable, without considering other factors, such as pain, itching, etc. The undeniable advantages of using the AGHO of the invention and its topical compositions are evident, this new result being of particular relevance due to the shortening of the healing times, the lack of bothersome or irritating side effects, such as exudations, itching and sores, and in the case of using honey, a sticky mess in the area under treatment. (*) and (**) In the cases summarized in the following table where it is a single wound, it was only possible to control the times with the application of the AGHO of the invention, knowing from previous experience that traditional treatments take at least twice as long. Obtaining AGHO formulations with the various active components:

[0073] To obtain the following formulations of the present invention, an example is given below with each of the plant species (herbs) listed above, using a variety of polyunsaturated oils, as well as a single example that uses a dispersion of nanoparticles of a colloidal metal with collagen.

[0074] Procedure for obtaining a topical composition of AGHO with MINT. It has antispasmodic, carminative, antiseptic, analgesic, anti-inflammatory, and stimulating properties. 180 gr. of finely ground dry and properly conditioned Spearmint leaves are soaked in 10 liters of sunflower oil and placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process between 70 and 85 ° C for 9 hours, allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0 ° C from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Spearmint, shaking and stirring for 10 minutes, resulting in a concentration of 3% of the phyto-therapeutic active ingredient / liter in vegetable oil for immediate packaging.

[0075] Procedure for obtaining a topical composition of AGHO with Hypericum Perforatum: This herb increases the skin's photosensitivity. It is antibacterial and anti-inflammatory. Optimal for treating sun-damaged and blemished skin. Highly beneficial for treating eczema and dermatitis. Traditionally, St. John's wort has been used in numerous European countries to treat genitourinary or bronchial inflammation, to relieve nervous system disorders, and as a healing agent for bruises and burns. 200 g of palm oil are soaked in 10 liters of palm oil.of Hypericum Perforatum petals, in a dry state and properly conditioned, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 80ºC for 10 hours, allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC of the AGHO, the enriched oil is mixed with the extract of the active ingredient of Hypericum Perforatum by shaking and stirring for 12 minutes, resulting in a concentration of 4% of the phytotherapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0076] Procedure for obtaining topical composition of AGHO with Rosa Eglanteria (Rosehip). This herb is rich in essential fatty acids and acts to regulate skin elasticity and restore hydration. 250 grams of dried and properly conditioned rosehip petals are soaked in 10 liters of olive oil and placed inside a mesh bag made of a material permeable to the passage of liquids and capable of retaining solid particles. The oil is heated to 75°C for 13 hours, allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped within. During the thawing process, at approximately 0°C, the enriched oil is mixed with the rosehip active ingredient extract and stirred for 10 minutes, resulting in a concentration of 5% of the phytotherapeutic active ingredient per liter of vegetable oil, ready for immediate packaging.

[0077] Procedure for obtaining topical AGHO composition with Equesitum Arbense (Horsetail): Horsetail is a plant used to reduce fluid retention (edema). 250 gr. of Horsetail stems, crushed in a dry state and properly conditioned, are soaked in liters of coconut oil, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 11 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it, and during the thawing process; at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Horsetail and stirring for 10 minutes, resulting in a concentration of 4.5% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0078] Procedure for obtaining a topical composition of AGHO with Cannabis. It has properties as an analgesic, immunosuppressant, muscle relaxant, anti-inflammatory, antineoplastic, and anti-allergenic agent. 150 g of shredded, dry, and properly conditioned leaves are soaked in 10 liters of soybean oil and placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 75°C for 11 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped inside. During the thawing process, At approximately 0ºC in the AGHO, the enriched oil is mixed with the extract of the active ingredient of Cannabis, stirring for 10 minutes, resulting in a concentration of 4% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0079] Procedure for obtaining a topical composition of AGHO with Butcher's broom. Butcher's broom is a refreshing, healing, and anti-ulcer herb. 250 grams of dried and properly conditioned flowers are soaked in 10 liters of corn oil and placed inside a mesh bag with a mesh of 1.5 mm. material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 70ºC for 8 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it, and during the thawing process; at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the Carnicera active ingredient, stirring for 10 minutes, resulting in a concentration of 4% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0080] Procedure for obtaining the topical composition of AGHO with Barley: Barley has a high vitamin C content. It helps strengthen the immune system. At the same time, it can be beneficial for improving the management of chronic conditions. It improves the development of body cells. Barley is an excellent source of selenium. This mineral helps maintain skin elasticity. 280 g of sesame oil is soaked in 10 liters of sesame oil.of properly conditioned barley grains with hulls, placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 85°C for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0°C of the AGHO, the enriched oil is mixed with the extract of the active ingredient of Barley, stirring by. 10-minute period, resulting in a concentration of 4% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0081] Procedure for obtaining topical AGHO composition with flaxseed: Flaxseed has a robust antioxidant content and (among other properties) has shown promise in reducing the risk of diabetes. In 10 liters of peanut oil, 280 g.of crushed seeds with flaxseed husks, properly conditioned, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 85ºC for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC of the AGHO, the enriched oil is mixed with the extract of the active ingredient of Flaxseed, stirring for 10 minutes, resulting in a concentration of 4.5% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0082] Procedure for obtaining topical composition of AGHO with Alfalfa: Alfalfa purifies the body and promotes the elimination of toxins (among other properties). In 10 liters of rice oil, 300 stalks of grass, cut into pieces of approximately 1.5 cm, properly conditioned, are placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a process of heating at 70ºC for 8 hours; it is allowed to cool, and then the frozen oil is extracted with the trapped O2 and O3 microbubbles. During the thawing process, at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Alfalfa, stirring for 10 minutes, resulting in a concentration of 2.5% of the phytotherapeutic active ingredient / liter of vegetable oil for immediate packaging.

[0083] Procedure for obtaining a topical composition of AGHO with Boldo: Boldo is a good anti-inflammatory (among other properties). In 10 liters of linseed oil, 300 pieces of broken, dried Boldo leaves, cut into pieces of approximately 1.5 cm, properly conditioned, are placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 70°C for 8 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped in it. During the thawing process, at approximately 0°C from the AGHO, the enriched oil is mixed with the extract of the Boldo active ingredient, stirring for 10 minutes, resulting in a concentration of 3% of the phytotherapeutic active ingredient per liter of vegetable oil for immediate packaging.

[0084] Procedure for obtaining topical composition of AGHO with Chamomile: Chamomile is a good anti-inflammatory, anti-irritant, and stabilizer for sensitive skin (among other properties). 250 dried chamomile flowers, properly conditioned, are placed in 10 liters of safflower oil, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 80°C for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped in it. During the thawing process, at approximately 0°C from the AGHO, the enriched oil is mixed with the extract of the chamomile active ingredient, stirring for 10 minutes, resulting in a concentration of 4% of the phytotherapeutic active ingredient per liter of vegetable oil for immediate packaging.

[0085] Procedure for obtaining topical composition of AGHO with Laurel: Laurel prevents infections and various types of tumors (among other properties). In 10 liters of rapeseed oil, place 150 gr. of dried bay leaves, crushed into pieces of 2 cm average, properly conditioned, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 80 ° C for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0 ° C From AGHO, the enriched oil is mixed with the extract of the active ingredient of Laurel, stirring for 10 minutes, resulting in a concentration of 5% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0086] Procedure for obtaining topical AGHO composition with Aloe vera: Aloe vera, among its many properties, is used for wound healing. 150g of gelatinous pulp cut into 2 cm pieces, properly conditioned, are placed in 10 liters of almond oil and placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 75°C for 8 hours; it is allowed to cool, and then the frozen oil is extracted with the O micro-bubbles. 2 I 3trapped in it, and during the thawing process; at approximately 0°C in the AGHO, the oil enriched with the extract of the active ingredient of Aloe vera is mixed, stirring for 10 minutes, resulting in a concentration of 5% of the phytotherapeutic active ingredient / liter of vegetable oil for immediate packaging.

[0087] Procedure for obtaining a topical composition of AGHO with Olive: Olive leaves, among other properties, are anti-inflammatory, with antibacterial, antiparasitic, antiseptic, and antifungal properties. 200g of dried and broken olive leaves are added to 10 liters of olive oil. properly conditioned, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC of the AGHO, the enriched oil is mixed with the extract of the active ingredient of the Olivo vera tree, stirring for 10 minutes, resulting in a concentration of 4.5% of the phyto-therapeutic active ingredient / liter in vegetable oil for packaging.

[0088] Procedure for obtaining AGHO topical composition with Calendula: Calendula, among its many properties, is an anti-inflammatory, healing agent, and tissue regenerator. 250g of properly conditioned Calendula flower petals are placed in 10 liters of grape seed oil and placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 85°C for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the O micro-bubbles. 2 I 3 trapped in it, and during the thawing process; at approximately 0°C in the AGHO, the oil enriched with the calendula extract is mixed, stirring for 10 minutes, resulting in a concentration of 4.5% of the phytotherapeutic active ingredient / liter of vegetable oil, for immediate packaging.

[0089] Procedure for obtaining AGHO composition with Comfrey: Comfrey attacks skin problems such as boils, psoriasis, etc., as an emollient and healing agent, among other various properties. In 10 liters of walnut oil, 250gr. of Comfrey flower petals are placed inside a bag whose mesh is permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 80ºC for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it, and during the thawing process; At approximately 0ºC in the AGHO, the enriched oil is mixed with the extract of the active ingredient of Comfrey, stirring for 10 minutes, resulting in a concentration of 4.5% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0090] Procedure for obtaining topical AGHO composition with Manuka: Manuka honey has antibiotic properties especially suitable for treating burns. 250g of Manuka honey is dissolved in 10 liters of sunflower oil. The oil is heated to 75ºC for 8 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it. During the thawing process; at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Manuka honey by mixing and shaking for 10 minutes, resulting in a concentration of 4% of the phytotherapeutic active ingredient / liter in vegetable oil for immediate packaging.

[0091] Procedure for obtaining topical AGHO composition with Saccharum officinarum L: Saccharum officinarum L (Sugarcane) softens tumors and abscesses. 250g of sugarcane stalks, duly conditioned in 2 cm pieces, are placed in 10 liters of linseed oil, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 85ºC for 11 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it, and during the thawing process; At approximately 0ºC in the AGHO, the enriched oil is mixed with the extract of the active ingredient of Sugar Cane, stirring for 10 minutes, resulting in a concentration of 3% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0092] Procedure for obtaining a topical composition of AGHO with Beta vulgaris: Beta vulgaris or Sugar Beet is rich in polyphenols; anthocyanins; and has good antioxidant activity. 300 gr. of dried Beta vulgaris flower petals are placed in 10 liters of corn oil and placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 85ºC for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it, and during the thawing process; at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Sugar Beet, stirring for 10 minutes, resulting in a concentration of 3% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0093] Procedure for obtaining a topical composition of AGHO with Lavender: Lavender is basically (among its properties) a sedative. 300 g of dry flower petals are placed in 10 liters of palm oil and placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 85°C for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it. During the thawing process, at approximately 0°C from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Lavender for 10 minutes, resulting in a concentration of 3% of the phytotherapeutic active ingredient per liter of vegetable oil for immediate packaging.

[0094] Procedure for obtaining topical AGHO composition with Centella Asiatica: Centella Asiatica is basically an analgesic, antimicrobial, and antiviral agent useful for the treatment of skin conditions. 300 grams of the flower petals, properly conditioned in a dry state, are placed in 10 liters of coconut oil and placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 75°C for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped in it. During the thawing process, at approximately 0°C from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Centella Asiatica for 10 minutes, resulting in a concentration of 3.5% of the phytotherapeutic active ingredient / liter in vegetable oil for immediate packaging.

[0095] Procedure for obtaining topical AGHO composition with Malva. Malva is basically an emollient, anti-inflammatory and antioxidant. 300 gr. of the flower petals, properly conditioned in a dry state, are placed in 10 liters of palm oil and placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the process of Defrosting; at approximately 0°C, the enriched oil is mixed with the extract of the active ingredient of Malva for 10 minutes, resulting in a concentration of 3.5% of the phytotherapeutic active ingredient / liter of vegetable oil, which is then immediately packaged.

[0096] Procedure for obtaining a topical composition of AGHO with witch hazel: Witch hazel is primarily an astringent, antiphlogistic, and hemostatic agent. It is useful in treatments, especially topical ones, for superficial skin lesions, eczema, burns, varicose veins, and hemorrhoids. 300 g are added to 10 liters of soybean oil.of the flower petals in a dry state, and placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC of the AGHO, the enriched oil is mixed with the extract of the active ingredient of Hamamelis for 10 minutes, resulting in a concentration of 3.5% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0097] Procedure for obtaining topical composition of AGHO with Arnica: Arnica has anti-inflammatory and analgesic properties, with healing properties for the skin. 300 grams of dried arnica flower petals are placed in 10 liters of soybean oil and placed inside a mesh bag made of a material permeable to the passage of liquids and capable of retaining solid particles. The oil is heated to 75°C for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped within. During the thawing process, at approximately 0°C, the AGHO is mixed with the Arnica active ingredient extract for 10 minutes, at a concentration of 3.5% of the phytotherapeutic active ingredient per liter of vegetable oil, before immediately packaging.

[0098] Procedure for obtaining topical AGHO composition with Yarrow: Yarrow has antibacterial properties, and is used externally to wash wounds of any nature. 300 gr. of dry flower petals are placed in 10 liters of peanut oil and placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it, and during the thawing process; at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Yarrow by space 10 minutes, at a concentration of 3.0% of the phytotherapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0099] Procedure for obtaining topical AGHO composition with Sage: Sage has antibacterial properties, and is used externally to wash wounds of any nature. In 10 liters of linseed oil, 300 gr. of dry flower petals are placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75 ° C for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0 ° C from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Sage for 10 minutes, at a concentration of 3.0% of the phytotherapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0100] Procedure for obtaining topical composition of AGHO with Thyme: Thyme has antibiotic, antibacterial, antiviral, and anti-inflammatory properties. 300 gr. of dry flower petals are placed in 10 liters of rapeseed oil and placed inside a bag with a mesh made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 10 hours; it is allowed to cool, and The frozen oil is then extracted with the O2 and O3 microbubbles trapped within it, and during the thawing process, at approximately 0°C in the AGHO, the enriched oil is mixed with the extract of the active ingredient of Thyme for 10 minutes, at a concentration of 2.0% of the phytotherapeutic active ingredient / liter in vegetable oil, before immediately proceeding to packaging.

[0101] Procedure for obtaining a topical composition of AGHO with Tea Tree: Tea Tree has antibacterial and anti-inflammatory properties. 200 g of dried, shredded leaves are placed in 10 liters of jojoba oil, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is heated to 75°C for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped in it. During the thawing process, at approximately 0°C from the AGHO, the enriched oil is mixed with the extract of the Tea Tree active ingredient for 10 minutes, at a concentration of 2.0% of the phytotherapeutic active ingredient / liter in vegetable oil, before immediately proceeding to packaging.

[0102] Procedure for obtaining topical composition of AGHO with Rosemary: Rosemary has antibacterial, antiseptic, fungicidal, and balsamic properties, with a rubefacient and healing effect by increasing blood flow to the localized area. 200 grams of dried, shredded leaves and flowers are placed in 10 liters of almond oil, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is heated to 75°C for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped in it. During the thawing process, at approximately 0°C of the AGHO, the enriched oil is mixed with the rosemary active ingredient extract for 10 minutes, at a concentration of 2.2% of the phytotherapeutic active ingredient / liter in vegetable oil, before immediately packaging.

[0103] Procedure for obtaining topical composition of AGHO with Chickweed: Chickweed has healing properties, soothes itching, is anti-inflammatory, and astringent. 200 gr. of a mixture of stems, leaves, and flowers, shredded and dry, are placed in 10 liters of rice oil and placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC from the AGHO, the oil enriched with the extract of the active ingredient of Chickweed for 10 minutes, at a concentration of 2.4% of the phyto-therapeutic active ingredient / liter in vegetable oil, for immediate packaging.

[0104] Procedure for obtaining topical composition of AGHO with Plantain: Plantain has antiseptic, astringent, anti-inflammatory, healing, vulnerary, and purifying properties in the treatment of varicose ulcers, sores, and pustules. 250 gr. are placed in 10 liters of grape seed oil.of stems and leaves, shredded and dry, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC of the AGHO, the enriched oil is mixed with the extract of the active ingredient of the Plantain for 10 minutes, in a concentration of 2.4% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0105] Procedure for obtaining a topical composition of AGHO with Shepherd's Purse: Shepherd's Purse has astringent and soothing properties and is an effective herbal remedy for various skin conditions. 270 grams of dried, crushed flowers are placed in 10 liters of sesame oil and placed inside a mesh bag made of a permeable material. of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 75ºC for 9 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0ºC from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Shepherd's Purse for 10 minutes, in a concentration of 2.7% of the phyto-therapeutic active ingredient / liter in vegetable oil to proceed immediately to packaging.

[0106] Procedure for obtaining topical AGHO composition with Ceibo: Ceibo helps wound healing and has anti-inflammatory properties for tumors. In 10 liters of walnut oil, 290 grams of the tree bark, crumbled into 2 cm pieces and dry, are placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles. The oil is subjected to a heating process at 90 ° C for 12 hours; it is allowed to cool, and then the frozen oil is extracted with the O2 and O3 micro-bubbles trapped in it. During the thawing process, at approximately 0 ° C from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Ceibo for 10 minutes, at a concentration of 2.3% of the phytotherapeutic active ingredient / liter in vegetable oil, before proceeding immediately to packaging.

[0107] Procedure for obtaining topical composition of AGHO with Oregano: Oregano has analgesic and antiseptic effects. 260 grams of dried, shredded leaves are placed in 10 liters of jojoba oil and placed inside a mesh bag made of a material permeable to the passage of liquids and capable of retaining solid particles. The oil is heated to 70°C for 10 hours. It is allowed to cool, and then the frozen oil is extracted with the O2 and O3 microbubbles trapped within. During the thawing process, at approximately 0°C, the AGHO is mixed with the oregano extract for 10 minutes, at a concentration of 2.3% of the phytotherapeutic active ingredient per liter of vegetable oil, before immediately packaging.

[0108] Procedure for obtaining a topical composition of AGHO with Pennyroyal: Pennyroyal has effects where tissues suffer injuries due to an insufficient supply of silicic acid. 280 gr. of shredded leaves and dried flowers are placed in 10 liters of sunflower oil, placed inside a bag with a mesh material permeable to the passage of liquids and capable of retaining solid particles; the oil is subjected to a heating process at 70 ° C for 10 hours; it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped in it, and during the thawing process; at approximately 0 ° C from the AGHO, the enriched oil is mixed with the extract of the active ingredient of Pennyroyal for 10 minutes, at a concentration of 2.8% of the phytotherapeutic active ingredient / liter of vegetable oil.

[0109] Procedure for obtaining a topical AGHO composition with collagen and colloidal nanoparticles. For each liter of AGHO of the invention, 2% to 5% of collagen is suspended, in whose mass a suspension of silver, zinc or copper nanoparticles with a concentration of 100 to 140 ppm has previously been made. It is mixed cold with stirring for 30 minutes until a good dispersion of the nanoparticles in the collagen is achieved. Once this dispersion is achieved, the collagen is added with the dispersion, cold, to the AGHO, stirring slowly until the collagen emulsion in the AGHO is achieved.

[0110] Packaging or conditioning of the formulations of the invention, ready for use. Preferably, although this does not exclude other forms of presentation, the topical solutions are packaged in an aerosol, under pressure and without contact with atmospheric air, to ensure a longer shelf life of the product by reducing the oxidation rate of the active ingredient(s). The AGHO oil solution is placed in an airtight container under internal pressure in an airless atmosphere, using a propellant that is inert to the oxidation of the active ingredient and before further oxidation of the active ingredient in the selected herb occurs. This aerosol container, using a propellant gas and in the absence of oxygen, is capable of creating a flow of micro-droplets that are propelled by the pressure generated by the propellant inside the container, allowing the topical composition to reach places where access would otherwise be impossible due to dermal absorption. The packaging guarantees the preservation of the formula from the product's production until the end of the container's contents by being deprived of atmospheric oxygen, i.e., oxidation of the active ingredient is prevented and the fluid's thermodynamic equilibrium conditions are maintained. The 60 ml aerosol container is recommended to be applied by placing the container 15 cm from the surface to be treated, pressing the valve, ensuring the formation of a curtain of microparticles that, when propelled by a propellant, allows the product to reach places where other products could not.

[0111] Through the aforementioned tests, given as non-limiting examples of the scope of the invention, and clinical evaluation, it was possible to demonstrate that the product of the present invention is not cytotoxic, nor irritating nor sensitizing, which makes them fully biocompatible and at the same time it was possible to demonstrate the new result obtained which is evidenced in the reduction of healing and / or scarring times, without itching or irritation, having confirmed that the scarring, that is, the healing of the lesion, is definitive.

Claims

1 / 3 CLAIMS 1. PROCEDURE FOR OBTAINING HYPEROXYGENATED FATTY ACIDS FOR MEDICAL USE, which includes as a first step the use of an O2 concentrator, by means of which, by entering a flow of atmospheric air between 1 to 5 liters / minute, at ambient temperature and pressure, an equal volume of N2-free air with an O2 concentration between 90% and 98% is achieved at its exit; this air stream enriched with O2 is sent through a UV emitter, from which it exits with a quantity of 10 to 17 gr. of O3 / liter of air, characterized in that the output of said UV emitter constituted by air + O2 + O3, with the aforementioned quantity of 10 to 17 gr. of O3 / liter of air, is entered into a conduit submerged inside a container with a vegetable oil, chosen from mono and polyunsaturated oils with a range of C16 to C18; O16 to O13 and H 16 to H 20; said duct has at one end an emitter of micro-bubbles of the enriched air that expands within the mass of vegetable oil, at the same time as said container is subjected to a slow cooling of 5 to 8 hours, until reaching a temperature range of -25ºC to -12ºC, achieving the stabilization and retention of O3 within the solid mass of the vegetable oil. 2- PROCEDURE FOR OBTAINING HYPEROXYGENATED FATTY ACIDS FOR MEDICAL USE, as claimed in 1, characterized in that the air stream enriched with O2 is sent through a UV emitter consisting of at least one UV radiation emitting lamp with a device for cooling the air stream downstream, the same having a quantity of 15gr. of O3 / liter of air. 2 / 3 3- PROCEDURE FOR OBTAINING HYPEROXYGENATED FATTY ACIDS FOR MEDICINAL USE, as claimed in 1, characterized in that the vegetable oil is chosen from one or more olive oils (fruit of the olive tree); g; sunflower; soybean; palm, both fruit and seed; coconut; sesame; peanut; rice; corn; flax (linseed oil); safflower; hemp or marijuana; rapeseed; almond; walnut; hazelnut; grape seeds; poppy seeds. 4- MEDICINAL FORMULATIONS FOR THE PREVENTION AND TREATMENT OF WOUNDS, which uses AGHO obtained as claimed in 1 to 3, characterized in that the active ingredients are extracted between 150 to 300g of at least one of the following plant species, for every 10 liters of AGHO, in an oil solution of a herb, chosen from Peppermint, Hypericum Perforatum, Rosa Eglanteria, Equesitum Arbense, Cannabis, Carnicera, Barley, linseed, alfalfa, plantain, boldo.Chamomile, bay leaf, Aloe vera, olive, Calendula, Comfrey, Manuka, Saccharum officinarum L, Beta vulgaris, Lavender, Centella asiatica, Mallow, Witch hazel, Arnica, Yarrow, Sage, Thyme, Tea tree, Rosemary, Chickweed, Plantain, Shepherd's purse, Calendula, Ceibo, Oregano, Pennyroyal with a total concentration of the phytotherapeutic active ingredient between 2% to 5% per total liter of oil. 5- MEDICINAL FORMULATIONS FOR THE PREVENTION AND TREATMENT OF WOUNDS, as claimed in 4, characterized in that two or more active ingredients of the aforementioned herbs are mixed with the AGHO, up to a total of 5% per liter of oil. 3 / 3 6- MEDICINAL FORMULATIONS FOR THE PREVENTION AND TREATMENT OF WOUNDS, as claimed in 1 to 3, characterized by having in an AGHO, a suspension of 2% to 5% of collagen with which nanoparticles of Silver, Zinc or Copper are mixed with a concentration of 100 to 140 ppm 7- PROCEDURE FOR OBTAINING FORMULATIONS CONTAINING SAID AGHO ACIDS as claimed in 4 to 5, characterized in that 150 to 300 Gr. of one of the aforementioned herbs in a dry state are immersed in 10 liters of at least one of the aforementioned vegetable oils, placed inside a bag whose mesh is made of a material permeable to the passage of liquids and essential oils and capable of retaining solid particles;the oil is subjected to a heating process between 70ºC to 85ºC for 8 to 12 hours, it is allowed to cool, and then the frozen oil is extracted with the micro-bubbles of O2 and O3 trapped therein, and during the thawing process, at approximately 0ºC, the enriched oil is mixed with the extract of the active ingredient of the selected herb, shaking and stirring for 10 to 15 minutes, to immediately proceed to package the oil in an air-free atmosphere and before further oxidation of the active ingredient of the selected herb occurs, inside an airtight container equipped with a spray nozzle capable of diffusing the composition forming micro-drops of high impact on the tissues, said container having inside a propellant inert to the oxidation of the active ingredient.;

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