Antibody linker conjugates

Abstract

Described herein are novel linkers for use with immunoconjugates to connect a targeting moiety to a radiochelator moiety without impairing the functionality of either. The linkers of the invention may provide a stable linkage in circulation, permit fast total body clearance including from organs such as the liver, high tumor uptake and high tumor-to-normal-organ radiation exposure (for example, in the liver and kidneys). The immunoconjugates can be used to deliver radioisotopes including alpha and beta emitters for the treatment of tumors or cancer.

Description

ANTIBODY LINKER CONJUGATESCROSS REFERENCE

[0001] This application claims the benefit of U.S. Provisional Application 63 / 556,300 filed on February 21, 2024 which is hereby incorporated by reference in its entirety.SEQUENCE LISTING

[0002] The instant application contains a Sequence Listing which has been submitted electronically in XML format and is hereby incorporated by reference in its entirety. Said XML copy, created on February 18, 2025, is named 60924-729.601_SL.xml and is 74,571 bytes in size.BACKGROUND

[0003] The exquisite specificity of antibodies, such as IgGs, to their antigens makes antibodies a premier targeting platform for therapeutics; however, the typical serum half-life of at least three weeks for an IgG is disadvantageous for the delivery of radioisotopes including alpha-emitting isotopes such as actinium-225 (225Ac) and beta-emitting isotopes such as lutetium-177 (177Lu) and yttrium-90 (90Y), in particular due to prolonged exposure and chronic off-target toxicities. The advent of engineered smaller antibody formats (e.g., monomeric scFv’s, heavy-chain only antibodies, or single-domain antibody fragments) provides the exquisite specificity of a full-size antibody (e.g. an IgG (-150 kDa)) in a smaller format (e.g. 15 to 30 kDa) and with a much shorter serum half-life (e.g., 30 minutes to 2 hours). Unfortunately, these short half-lives may not allow sufficient time for efficacious target binding due to poor retention and tumor uptake, and furthermore plasma clearance of these small antibody formats by the renal system can lead to isotope accumulation in renal tissues and problematic off-target toxicities.

[0004] The use of immunoconjugates comprising an antigen binding region coupled to an Fc or heavy chain constant region (e.g., 60 to 110 kDa) permits tuning pharmacokinetic (PK) properties to achieve an ideal balance of tumor uptake and penetration while avoiding rapid renal excretion, high kidney uptake and mitigating other systemic radiotoxicities such as myelosuppression. The use of antigen binding region coupled to a heavy chain constant region can shift the catabolism and elimination of the immunoconjugate from the kidney to the liver. While this can increase total body clearance of activity to reduce systemic radioactive exposure arising from the immunoconjugates, this can also shift radioactive exposure to the liver, a known major site of antibody clearance and catabolism.

[0005] There remains a need for immunoconjugates that can selectively deliver therapeutic levels of radioisotopes to cancer cells by further optimization and tuning of their PK properties bymodifying the linkers connecting the antigen binding and immunoglobulin heavy chain constant region to the radiochelator. Surprisingly and unexpectedly, the linkers described herein connect a targeting moiety to a radiochelator moiety without impairing the functionality of either, provide a stable linkage in circulation, permit fast total body clearance, high tumor uptake and high tumor- to-normal-organ radiation exposure (for example, in the liver and kidneys) and promote faster liver clearance in comparison to conventional linker systems in the prior art for use with immunoconjugates. The immunoconjugates described herein allow for the selective delivery of therapeutic levels of radioisotopes to cancer cells with high- and low-expressing target receptors.SUMMARY

[0006] The present invention relates to immunoconjugates or radioimmunoconjugate, compositions comprising the same, and methods of using such immunoconjugates and compositions. The immunoconjugates and compositions of the present invention have numerous uses, e.g., for delivery of a radioisotope to kill a target cell (e.g. a cancer cell expressing a target antigen bound by the radioimmunoconjugate); for detection and characterization of malignant cells within a subject (e.g., target antigen expression); and for diagnosis and treatment of a variety of diseases and conditions, such as, e.g., cancers, tumors, and other growth abnormalities involving antigen-expressing cells.

[0007] The present invention addresses a number of challenges inherent in the targeted delivery of alpha and beta particle emitters in vivo through the selection and particular combination of specific delivery platform components. The alpha and beta particle emitting radioisotope-delivery platforms of the present invention provide shorter half-lives compared to traditional IgGs, but longer half-lives than smaller monomeric antibody fragment formats. Such half-lives allow for a reduction in toxicity due to the alpha or beta emitter, while preserving the antibody fragment long enough in the body to exert therapeutic activity. For example, the alpha and beta particle emitting radioisotope-delivery platforms of the current disclosure exhibit enhanced tumor targeting and reduced accumulation in radiosensitive tissues such as the bone- marrow and kidney. Further, the alpha and beta particle emitting radioisotope-delivery platforms of the present invention containing the linkers described herein can provide a stable linkage in circulation, permit faster total body clearance, high tumor uptake and high tumor-to-normal-organ radiation exposure (for example, in the liver and kidneys) and faster clearance out of the liver than conventional linker systems in the prior art for use with immunoconjugates.

[0008] Further and surprisingly, the alpha and beta particle emitting radioisotope-delivery platforms of the present invention exhibit excellent tumor binding and labeling properties fortumors with different antigen densities, which can be a limitation for some use of some immunoconjugates.

[0009] Described herein in one aspect is an immunoconjugate comprising an: a) antigen binding region; b) an immunoglobulin heavy chain constant region; and c) a chelating agent; wherein the molecular weight of the immunoconjugate is between 60 and 110 kDa. In certain embodiments, the antigen binding region comprises an scFv polypeptide or a VHH polypeptide. In certain embodiments, the antigen binding region comprises an scFv polypeptide. In certain embodiments, the antigen binding region comprises a VHH polypeptide. In certain embodiments, the antigen binding region is humanized.

[0010] In an aspect, described herein is a compound of Formula (I), or a pharmaceutically acceptable salt thereof:Formula (I) wherein: at least one tertiary amide is present in the linear chain connecting R1to R8;R1is a radionuclide chelating moiety or a radionuclide complex thereof;X1is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-; m is 1, 2, 3, 4, 5, or 6; x is 0 or 1;R2is hydrogen, halogen, substituted or unsubstituted Ci-Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), -NRa-(substituted or unsubstituted Ci-Cealkyl), or substituted or unsubstituted Ci-Cioheteroalkyl, -©-(substituted or unsubstituted Ci- Cioheteroalkyl), -NRa-(substituted or unsubstituted Ci- Cioheteroalkyl), or C4- ©2opolyethylene glycol;X2is absent, -O-, -S-, -S(=O)2-, -NRa-, or -C(=O)N(Ra)-;L1is absent, Ci-Cioalkylene, ©2-Cioalkenylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, - S-, -S(=O)-, -S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, - NRbC(=O)-, -C(=O)NRb-, -OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, - NRbC(=N-Rb)NRb-, or -NRbC(=O)O-;L2is absent,Y1is absent,n is 0 or 1; each R3and R6is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -C(=O)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carboxyglutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with - C(=O)(unsubstituted or substituted Ci-C2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl-3-bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or -C(=0)NH-(C4-C2opolyethylene glycol); y is 0 or 1;t is 0 or 1; each Ra, Rb, Rc, Rd, R2, R4, R4a, R5, R7and R7ais independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci- Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, -La-(Re), or R3; each u is independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; each Lais independently absent or Ci-Cealkylene;Reis substituted or unsubstituted Cs-Cncycloalkyl, substituted or unsubstituted C2- Cnheteroycloalkyl, substituted or unsubstituted phenyl, or substituted or unsubstituted pyridinyl; or R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl; or R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl;X4is absent, -CH2-, -O-, -S-, or -NRC-; q is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;R8is a moiety capable of covalently conjugating with a biologic targeting moiety (RB);

[0011] In an aspect, described herein is a compound of Formula (I), or a pharmaceutically acceptable salt thereof:Formula (I)wherein: at least one tertiary amide is present in the linear chain connecting R1to R8;R1is a radionuclide chelating moiety or a radionuclide complex thereof;X1is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-; m is 1, 2, 3, 4, 5, or 6; x is 0 or 1;R2is hydrogen, halogen, substituted or unsubstituted Ci-Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), -NRa-(substituted or unsubstituted Ci-Cealkyl), or substituted or unsubstituted Ci-Cioheteroalkyl, -©-(substituted or unsubstituted Ci- Cioheteroalkyl), -NRa-(substituted or unsubstituted Ci- Cioheteroalkyl), or C4- ©2opolyethylene glycol;X2is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-;L1is absent, Ci-Cioalkylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, -S-, -S(=O)-, -S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, -NRbC(=O)-, -C(=O)NRb-, - OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, -NRbC(=N-Rb)NRb-, or - NRbC(=O)O-;n is 0 or 1; each R3and R6is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-©2opolyethylene glycol, -C(=0)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carboxyglutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with - C(=O)(unsubstituted or substituted Ci-©2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independentlyoptionally replaced with -C(=O)NH-(2,4,6-trimethyl-3-bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or -C(=0)NH-(C4-C2opolyethylene glycol); y is 0 or 1;t is 0 or 1; each Ra, Rb, Rc, Rd, R2, R4, R4a, R5, R7and R7ais independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci- Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, -La-(Re), or R3; each u is independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; each Lais independently absent or Ci-Cealkylene;Reis substituted or unsubstituted Cs-Cncycloalkyl, substituted or unsubstituted C2- Cnheteroycloalkyl or substituted or unsubstituted phenyl; or R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl; or R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl;X4is absent, -CH2-, -O-, -S-, or -NRC-; q is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;R8is a moiety capable of covalently conjugating with a biologic targeting moiety (RB); and whereinnot:

[0012] In some embodiments, RBis an antibody or fragment thereof, peptide, nucleic acid, or small molecule. In some embodiments, RBis an antibody or fragment thereof. In some embodiments, R8is a moiety capable of conjugating with an amine (-NH2) or thiol (-SH) of an antibody or fragment thereof.

[0013] In some embodiments, the compound of Formula (I) has the structure of Formula (la), (lb), (Ic), or a pharmaceutically acceptable salt thereof:Formula (Ic)wherein: X5is absent, -O-, -S-, -S(=O)-, -S(=O)2-, -NR9-, -C(=O)-, -NR9C(=O)-, - C(=O)NR9-, -C(=O)O-, -OC(=O)-, -OC(=O)NR9-, -NR9C(=O)NR9-, - NR9C(=S)NR9-, -NR9C(=O)O-; and each R9is independently selected from hydrogen, and Ci-C4alkyl.

[0014] In some embodiments, the compound of Formula (I) has the structure of Formula (Id), (le), (If), or (Ig), a pharmaceutically acceptable salt thereof:Formula (If), orFormula (Ig) wherein, X6is -NR9- or -(CH2)g-NR9-; and g is 1, 2, 3, or 4.

[0015] In some embodiments, R1is l,4,7,10-tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTA); l,4,7,10-tetraazacyclododecane-l,4,7-triacetic acid (DO3A); 1,4,7,10- tetraazacyclododecane-l,7-diacetic acid (DO2A); a,a',a",a"'-tetramethyl-l,4,7,10- tetraazacyclododecane- 1 ,4,7, 10-tetraacetic acid (DOTMA); 1 ,4,7, 10-tetrakis(carbamoylmethyl)- 1 ,4,7, 10-tetraazacyclododecane (DOTAM); 1 ,4,7, 10-tetraazacyclododecane- 1 ,4,7, 10-tetra- propionic acid (DOTPA); 2,2',2"-(10-(2-amino-2-oxoethyl)-l,4,7,10-tetraazacyclododecane- l,4,7-triyl)triacetic acid; 6,6'-(((pyridine-2,6-diylbis(methylene))bis((carboxymethyl)azanediyl))- bis(methylene))-dipicolinic acid (FLjpypa); 6,6',6",6"'-(((pyridine-2,6-diylbis(methylene))-bis-(azanetriyl))-tetrakis(methylene))tetrapicolinic acid (JHUpydpa); 2,2',2"-(l,4,7-triazacyclononane- l,4,7-triyl)-triacetic acid (NOTA); 6,6'-((ethane-l,2-diylbis((carboxymethyl)-azanediyl))- bis(methylene))dipicolinic acid (TUoctapa); or 3,6,9,12-tetrakis(carboxymethyl)-3,6,9,12- tetraazatetradecanedioic acid (TTHA), or a radionuclide complex thereof.radionuclide complex thereof. In somecomplex thereof.

[0017] In some embodiments, the compound of Formula (I) has the structure of Formula (II), or a pharmaceutically acceptable salt thereof:

[0018] In some embodiments, the compound of Formula (II) has the structure of Formula (III), or a pharmaceutically acceptable salt thereof:Formula (III) wherein:X2is absent, -CH2-, or -O-; and X3is absent, -CH2-, or -O-.

[0019] In some embodiments, the compound of Formula (I) has the structure of Formula (V), or a pharmaceutically acceptable salt thereof:Formula (V).

[0020] In some embodiments, the compound of Formula (V) has the structure of Formula (VI), or a pharmaceutically acceptable salt thereof:Formula (VI) wherein:X2is absent, -CH2-, or -O-; and X3is absent, -CH2-, or -O-.

[0021] In some embodiments, the compound of Formula (I) has the structure of Formula (VII), or a pharmaceutically acceptable salt thereof:Formula (VII) wherein:X2is absent, -CH2-, or -O-; X3is absent, -CH2-, or -O-; and

[0022] In some embodiments,thereof.

[0023] In some embodiments,radionuclide complex thereof.

[0024] In some embodiments, the radionuclide is a lanthanide or an actinide. In some embodiments, the radionuclide is a diagnostic or therapeutic radionuclide. In some embodiments, the radionuclide is an Auger electron-emitting radionuclide, a-emitting radionuclide, P-emitting radionuclide, or y-emitting radionuclide. In some embodiments, the radionuclide is a-emitting radionuclide. In some embodiments, the radionuclide is 225-actinium (225Ac), 213-bismuth (213Bi), 223-Radium (223Ra), 212-lead (212Pb), 111-indium (i nIn), 67-gallium (67Ga), 68-gallium (68Ga), 99m-technetium (99mTc), 90-yttrium (90Y), 177-lutetium (177Lu), 186-rhenium (186Re), 188-rhenium (188Re), 64-copper (64Cu), 67-copper (67Cu), 153-samarium (153Sm), 89-strontium (89Sr), 198-gold (198Au), 169-Erbium (169Er), 165-dysprosium (165Dy), 99m-technetium (99mTc), 89-zirconium (89Zr), or 52-manganese (52Mn). In some embodiments, the radionuclide is 225- actinium (225AC). In some embodiments, the targeting moiety is a polypeptide comprising an antigen binding region and an immunoglobulin heavy chain constant region, wherein the molecular weight of the polypeptide is between 60 and 110 kDa. In some embodiments, the targeting moiety binds to Delta-like protein 3 (DLL3), folate receptor alpha (FOLR1), Folate Receptor a (FRa), human epidermal growth factor receptor 2 (HER2), epidermal growth factor receptor (EGFR) insulin-like growth factor 1 (IGF-1) receptor, vascular endothelial growth factor (VEGF), or Tumor-associated calcium signal transducer 2 (TROP2), Angiopoietin, BCMA, CD19, CD20, CD22, CD25 (IL2-R), CD30, CD33, CD37, CD38, CD52, CD56, CD123 (IL-3R), cMET, DLL / Notch, EpCAM, FGF / FGF-R, GD2, Mesothelin, Nectin-4, PDGFRa, RANKL, SLAMF7, Claudin 18.2, or DKKl.

[0025] In another aspect, provided herein is a pharmaceutical composition comprising a compound described herein, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient. In some embodiments, the pharmaceutical composition is formulated for administration to a mammal by intravenous administration.

[0026] In another aspect, provided herein is a method of treating cancer in a mammal comprising administering to the mammal a compound described herein that is conjugated to a targeting moiety. In some embodiments, the mammal is a human. In some embodiments, thecancer comprises a glioma, thyroid cancer, lung cancer, colorectal cancer, stomach cancer, liver cancer, pancreatic cancer, renal cancer, prostate cancer, testis cancer, breast cancer, cervical cancer, endometrial cancer, ovarian cancer, or melanoma.

[0027] In another aspect, provided herein is a method for identifying tissues or organs in a mammal with a tumor comprising: i) administering to the mammal a radionuclide complex of the immunoconjugate described herein, and ii) performing positron emission tomography (PET) analysis, single-photon emission computerized tomography (SPECT), or magnetic resonance imaging (MRI); wherein the tumor expresses receptors that are targeted by the targeting moiety; wherein the radionuclide is a diagnostic radionuclide. In some embodiments, step (ii) is initiated after an amount of time following step (i) sufficient for interaction between the radionuclide complex of the immunoconjugate described herein.

[0028] These and other features, aspects and advantages of the present invention will become better understood with regard to the following description and appended claims. The aforementioned elements of the invention may be individually combined or removed freely in order to make other embodiments of the invention, without any statement to object to such combination or removal hereinafter.BRIEF DESCRIPTION OF THE DRAWINGS

[0029] FIG. 1 shows binding of the anti-DLL3 VHH-Fc construct.

[0030] FIG. 2A and 2B show binding to a DLL3-positive cell line (SHP-77).

[0031] FIG. 3 shows that DI 02 is internalized by SHP-77 and HEK-DLL3 cells.

[0032] FIG. 4A and 4B show the total whole body %ID for the two In-111 radioconjugates ofD102 and D138, respectively.

[0033] FIG. 4C, 4E and 4G show the total whole body %ID for 16 In-111 radioconjugates of D138 with reference to compound 1-1.

[0034] FIG. 4D and 4F show selected organ uptake values in %ID / g for 11 In-111 radioconjugates of D138 with reference to compound 1-1.

[0035] FIG. 4H shows representative maximum intensity projections (MIPs) of 5 In-111 radioconjugates of D138 with reference to compound 1-1.

[0036] FIG. 5A shows the total body %ID.

[0037] FIG. 5B shows the organ distribution and tumor uptake at 1, 3 and 6 d between the D138 conjugates. For each pair of bars in each organ on the x-axis, the bar on the left is for the D138 conjugate 3-1, and the bar on the right is for D138 conjugate 1-1.

[0038] FIG. 5C shows the ex vivo counts at the terminal timepoint (6 d), showing correspondence with the values calculated by imaging. For each pair of bars in each organ on the x-axis, the bar on the left is for the D138 conjugate 3-1, and the bar on the right is for D138 conjugate 1-1.

[0039] FIG. 6A shows the organ distribution and tumor uptake at 1, 3 and 6 d between the T157 conjugates. For each pair of bars in each organ on the x-axis, the bar on the left is for the T157 conjugate 3-1, and the bar on the right is for T157 conjugate 1-1.

[0040] FIG. 6B shows the ex vivo counts at the terminal timepoint (6 d), showing correspondence with the values calculated by imaging. For each pair of bars in each organ on the x-axis, the bar on the left is for the T157 conjugate 3-1, and the bar on the right is for T157 conjugate 1-1.

[0041] FIG. 7 showing the total %ID / g for each tissue collected at 10 min, 24 h, 72 h, 168 h, and 336 h. For each pair of bars in each organ on the x-axis, the bar on the left is for the D138 conjugate 3-1, and the bar on the right is for D138 conjugate 1-1.DETAILED DESCRIPTION

[0042] The present invention is described more fully hereinafter using illustrative, nonlimiting embodiments. This invention may, however, be embodied in many different forms and should not be construed as to be limited to the embodiments set forth below. Rather, these embodiments are provided so that this disclosure is thorough and conveys the scope of the invention to those skilled in the art. In order that the present invention may be more readily understood, certain terms are defined below. Additional definitions may be found within the detailed description of the invention.

[0043] In particular, in embodiments, the present invention addresses a number of challenges inherent in the targeted delivery of radioisotopes in vivo through the selection and particular assembly of specific immunoconjugate and radioimmunoconjugate components. The radioisotope-delivering platforms of the present invention provide shorter half-lives compared to traditional IgGs, but longer half-lives than smaller monomeric antibody fragment formats. In some embodiments, the subject radioisotope delivering platforms have a molecular size large enough (e.g., 60 kDa to 110 kDa) to substantially reduce off-target toxi cities, especially renal damage (e.g., from an alpha- or beta-emitting isotope cargo) and a small enough size for increased tissue penetration as compared to traditional IgGs, with maintained target specificity, and increased probability of first decay event in target tissue. In some embodiments, the subject radioisotope delivering platforms are useful for in vivo targeted delivery of radioisotopes (such as alpha- orbeta-emitters) safely and effectively by, in part, reducing certain adverse effects caused by platforms having half-lives over 5 days and / or molecular weights under 60 kDa. In some embodiments, the subject radioisotope delivering platforms are useful for in vivo targeted delivery of radioisotopes (such as alpha- or beta-emitters) safely and effectively, in part, by exhibiting decreased loss of targeting capacity due to radiolysis as compared to other possible delivery platforms. In some embodiments, the subject radioisotope delivering platforms are useful for in vivo targeted delivery of radioisotopes (such as alpha- or beta-emitters) safely and effectively, in part, by exhibiting increased stability in manufacturing under the temperatures required for certain radiolabeling processes (e.g., high temperature chelation with certain chelators) as compared to other possible delivery platforms using antibody fragments.Immunoconjugates

[0044] In one aspect, the invention provides immunoconjugates that specifically bind to a target antigen with high affinity. In some embodiments, the present invention provides an immunoconjugate that specifically binds to a cell-surface antigen of a cancer cell. In some embodiments, the immunoconjugate comprises three, four, five, six, or more CDRs or HVRs (Kabat). In some embodiments, the immunoconjugate binds a specific antigen and / or epitope with an affinity characterized by a KD of < 1 pM, < 100 nM, < 10 nM, < 1 nM, < 0.1 nM, < 0.01 nM, or < 0.001 nM (e.g. 10'8M or less, e.g. from 10'8M to 10'13M, e.g., from 10'9M to 10'13M).

[0045] The immunoconjugates described herein may serve as a platform for radio isotope delivery. Radioisotope delivering platforms are provided herein that have a relatively short halflife (e.g., less than one or two weeks but greater than two to eight hours).

[0046] In one embodiment, an immunoconjugate of the current disclosure comprises an: a) antigen binding region; b) an immunoglobulin heavy chain constant region; and c) a chelating moiety or a radionuclide complex thereof. In one embodiment an immunoconjugate of the current disclosure comprises an: a) antigen binding region; b) an immunoglobulin heavy chain constant region; and c) a chelating moiety or a radionuclide complex thereof; wherein the molecular weight of said immunoconjugate is between 60 and 110 kDa.

[0047] In one embodiment, an immunoconjugate of the current disclosure comprises an: a) VHH antigen binding region; b) an immunoglobulin heavy chain constant region; and c) a chelating moiety or a radionuclide complex thereof. In one embodiment an immunoconjugate of the current disclosure comprises an: a) VHH antigen binding region; b) an immunoglobulin heavy chain constant region; and c) a chelating moiety or a radionuclide complex thereof; wherein the molecular weight of said immunoconjugate is between 60 and 110 kDa.

[0048] In one embodiment, an immunoconjugate of the current disclosure comprises an: a) VHH antigen binding region; b) an immunoglobulin Fc region, together referred to as a VHH-Fc; and c) a chelating moiety or a radionuclide complex thereof. In one embodiment an immunoconjugate of the current disclosure comprises an: a) VHH antigen binding region; b) an immunoglobulin Fc region; and c) a chelating moiety or a radionuclide complex thereof; wherein the molecular weight of said immunoconjugate is between 60 and 110 kDa.

[0049] In one embodiment an immunoconjugate of the current disclosure comprises an: a) VHH antigen binding region; b) a variant immunoglobulin Fc region; and c) a chelating moiety or a radionuclide complex thereof. In one embodiment an immunoconjugate of the current disclosure comprises an: a) VHH antigen binding region; b) a variant immunoglobulin Fc region; and c) a chelating moiety or a radionuclide complex thereof; wherein the molecular weight of said immunoconjugate is between 60 and 110 kDa. In certain embodiments, the variant immunoglobulin Fc region comprises one or more amino acid alterations to reduce the serum or plasma half-life of the immunoconjugate.

[0050] In some embodiments, the radioisotope delivering platforms have sizes larger than about 60 kDa, in order to avoid certain toxicities from an alpha emitting isotope cargo, such as, e.g., off-target renal toxicities. In some embodiments, the radioisotope delivering platforms have sizes less than about 110 kDa in order to improve tumor penetration. In some embodiments, the radioisotope delivering platform has size between 60 and 110 kDa due to its dimeric structure of two individual antigen binding arms each having a VHH polypeptide fused to a hinge region and a wild-type or variant constant region. In some embodiments, the variant constant region has specific amino acid substitution(s) relatively to a wildtype Fc region in order to reduce half-life and / or eliminate Fc effector function(s).

[0051] In one embodiment, the antibody construct of the immunoconjugate consists of two antigen binding arms that are covalently linked to each other (for example via a disulfide linkage between associated heavy chain constant regions or immunoglobulin hinge regions). Each of the antigen binding arms independently consists of an antigen binding region, a hinge region, and a variant constant region. Within each antigen binding arm, the antigen binding region of the arm is covalently linked to the hinge region of the arm and the hinge region of the arm is covalently linked to the variant constant region of the arm, such that the hinge region is interposed between and thereby links the antigen binding region and the variant constant region within the antigen binding arm.

[0052] In a preferred embodiment, at least one of the two antigen binding regions in the immunoconjugate consists of one or two heavy chain only variable (VHH) polypeptides. In apreferred embodiment at least one of the two antigen binding regions consists of one VHH polypeptide. In a preferred embodiment, each of the two antigen binding regions of the immunoconjugate consists of one VHH polypeptide, which VHH polypeptides are the same or different.

[0053] In one embodiment, the antigen binding regions of the immunoconjugate bind to the same antigen. In one embodiment, the antigen binding regions of the immunoconjugate bind to different antigens. In one embodiment, the antigen binding regions of the immunoconjugate are the same. In one embodiment, the antigen binding regions of the immunoconjugate are different. In one embodiment, the antigen binding region of each antigen binding arm consists of one or two VHH polypeptides.

[0054] In one embodiment, the antigen binding region of one antigen binding arm consists of two VHH polypeptides and the antigen binding region of the other antigen binding arm does not comprise a VHH polypeptide. In one embodiment, the two antigen binding arms bind the same antigen. In one embodiment, the two antigen binding arms bind different antigens. In one embodiment, the two VHH polypeptides are the same. In one embodiment, the two VHH polypeptides are different. In one embodiment, the immunoconjugate is bispecific.

[0055] In one embodiment, the antigen binding region of one antigen binding arm consists of one VHH polypeptide and the antigen binding region of the other antigen binding arm consists of two VHH polypeptides. In one embodiment, the two antigen binding arms bind the same antigen. In one embodiment, the two antigen binding arms bind different antigens. In one embodiment, the three VHH polypeptides are the same. In one embodiment, two of the three VHH polypeptides are the same and are different from the third VHH polypeptide. In one embodiment, the three VHH polypeptides are different. In one embodiment, the immunoconjugate is bispecific.

[0056] In one embodiment, the antigen binding region of each antigen binding arm of the immunoconjugate consists of one VHH polypeptide. In one embodiment, the VHH polypeptides bind to the same antigen. In one embodiment, the VHH polypeptides bind to different antigens. In one embodiment, the VHH polypeptides are the same. In one embodiment, the VHH polypeptides are different. In one embodiment, the immunoconjugate is bispecific.Antigen Binding Regions

[0057] The antigen binding region confers specificity to the immunoconjugate and may suitably comprise a small antigen binding polypeptide. Such small antigen binding polypeptides confer advantages such as reducing the overall size of the immunoconjugate molecule allowing for tumor penetration and labeling. The small antigen binding polypeptide may lack certain regions dispensable for binding such as a light chain constant region, a heavy chain constantregion, a CHI region or a hinge region. In certain embodiments, the antigen binding region may lack a light chain variable region. In certain embodiments, the small antigen binding region may possess a molecular weight of between 10 kDa and 40 kDa.

[0058] In some embodiments, the small antigen binding region possesses a molecular weight of about 10 kDa to about 40 kDa. In some embodiments, the small antigen binding region possesses a molecular weight of about 10 kDa to about 15 kDa, about 10 kDa to about 20 kDa, about 10 kDa to about 25 kDa, about 10 kDa to about 30 kDa, about 10 kDa to about 35 kDa, about 10 kDa to about 40 kDa, about 15 kDa to about 20 kDa, about 15 kDa to about 25 kDa, about 15 kDa to about 30 kDa, about 15 kDa to about 35 kDa, about 15 kDa to about 40 kDa, about 20 kDa to about 25 kDa, about 20 kDa to about 30 kDa, about 20 kDa to about 35 kDa, about 20 kDa to about 40 kDa, about 25 kDa to about 30 kDa, about 25 kDa to about 35 kDa, about 25 kDa to about 40 kDa, about 30 kDa to about 35 kDa, about 30 kDa to about 40 kDa, or about 35 kDa to about 40 kDa. In some embodiments, the small antigen binding region possesses a molecular weight of about 10 kDa, about 15 kDa, about 20 kDa, about 25 kDa, about 30 kDa, about 35 kDa, or about 40 kDa. In some embodiments, the small antigen binding region possesses a molecular weight of at least about 10 kDa, about 15 kDa, about 20 kDa, about 25 kDa, about 30 kDa, or about 35 kDa. In some embodiments, the small antigen binding region possesses a molecular weight of at most about 15 kDa, about 20 kDa, about 25 kDa, about 30 kDa, about 35 kDa, or about 40 kDa.

[0059] The antigen binding region may comprise a VHH polypeptide, an scFv polypeptide, or a VNAR polypeptide. In certain embodiments, the antigen binding region comprises a VHH polypeptide. In certain embodiments, the antigen binding region comprises a ScFv polypeptide. In certain embodiments, the antigen binding region comprises a VNAR polypeptide. In certain embodiments, the antigen binding region is humanized.

[0060] The antigen region can comprise a specificity to an antigen selected by the skilled artisan to achieve a desired function, such as targeting a particular cancer, tumor, or cell type amenable to treatment with the described immunoconjugates or radioimmunoconjugates. As described herein antigen binding regions can be fragments or formats of antibodies known in the art. Intact antibodies can be engineered to conform to various small antigen binding region formats described herein (e.g., scFv). The antigen binding region may specifically bind to tumor antigen (e.g., an antigen specifically expressed or enriched in cancerous cells). In certain embodiments, the tumor antigen comprises Her2, Trop2, CEA, NaPi2b, uPAR, CDCP1, MUC-1, MUC-16, CEACAM-5, MR-1, Fnl4, MAGE-3, NY-ESO-1, EGFR, PDGFR, IGF1R, CSF-1R, PSMA, PSCA, STEAP-1, FAP, TEM8, 5T4, VEGFR, NRP1, CD19, CD20, CD22, CD25, CD30, CD33,CD37, CD38, CD39, CD44, CD47, CD52, CD70, CD71, CD74, CD79b, CD132, CD133, CD138, CD166, CD205, CD276, ROR1, ROR2, Glypican 3, Trail Receptor 2 (DR5), PD-L1, Mesothein, Bombesin, EpCAM, DARPP, CSPG4, Galectin-3, Integrin avpi, Integrin avP3, Integrin avP5, Integrin avP6, Integrin a5pi, Integrin alpha-3, Integrin alpha-5, Integrin beta-6, Nectin-4, Wnt activated inhibitory factor 1, DLL3, Transferrin Receptor, Folate Receptor alpha, Tissue Factor, BCMA, c-Met, LIV-1, AXL, AFP, ENPP3, CLDN6 / 9, DPEP3, RNF43, LRRC15, PTK7, P- cadherin, FLT3, EphA2, MTI-MMP, CXCR6, GD2, or Smoothened antigen (Smo). In certain embodiments, the tumor antigen comprises human epidermal growth factor receptor 2 (HER2), Delta-like ligand 3 (DLL3), folate receptor alpha (FOLR1), or Wnt activated inhibitory factor 1 (WAIF1). In certain embodiments, the tumor antigen comprises HER2. In certain embodiments, the tumor antigen comprises DLL3. In certain embodiments, the tumor antigen comprises FOLR1. In certain embodiments, the tumor antigen comprises WAIF1. In certain embodiments, the tumor antigen comprises TROP2. In certain embodiments, the tumor antigen comprises EGFR. In certain embodiments, the tumor antigen comprises PSA. In certain embodiments, the tumor antigen comprises MUC-1. In certain embodiments, the tumor antigen comprises CEA. In certain embodiments, the tumor antigen comprises NY-ESO-1.

[0061] In certain embodiments, the antigen binding region of the immunoconjugate comprises a sequence that is at least 85%, 90%, 95%, 97%, 98%, 99%, or 100% identical to the sequence set forth in SEQ ID NO: 20 and that binds to HER2.

[0062] In certain embodiments the antigen binding region of the immunoconjugate comprises: a) a CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 21; b) a CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 22; and c) a CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 23.

[0063] In certain embodiments, the antigen binding region of the immunoconjugate comprises a sequence that is at least 85%, 90%, 95%, 97%, 98%, 99%, or 100% identical to the sequence set forth in SEQ ID NO: 30 and that binds to DLL3.

[0064] In certain embodiments the antigen binding region of the immunoconjugate comprises: a) a CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 31; b) a CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 32; and c) a CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 33.

[0065] In some embodiments, the immunoconjugate of the present invention comprises a synthetically engineered antibody derivate, such as, e.g. a protein or polypeptide comprising an autonomous VH domain (such as, e.g., from camelids, murine, or human sources), single-domain antibody domain (sdAb), heavy-chain antibody domains derived from a camelid (VHH fragmentor VH domain fragment), heavy-chain antibody domains derived from a camelid VHH fragments or VH domain fragments, heavy-chain antibody domain derived from a cartilaginous fish, immunoglobulin new antigen receptor (IgNAR), VNAR fragment, single-chain variable (scFv) fragment, nanobody, “camelized” or “camelised” scaffold comprising a VH domain, Fd fragment consisting of the heavy chain and CHI domains, single chain FV-CH3 minibody, Fc antigen binding domain (Fcabs), scFv-Fc fusion, multimerizing scFv fragment (diabodies, triabodies, tetrabodies), disulfide-stabilized antibody variable (Fv) fragment (dsFv), disulfide-stabilized antigen-binding (Fab) fragment consisting of the VL, VH, CL and CHI domains, scFv comprising a disulfide- stabilized heavy and light chain (sc-dsFvs), bivalent nanobodies, bivalent minibodies, bivalent F(ab’)2 fragments (Fab dimers), bispecific tandem VHH fragments, bispecific tandem scFv fragments, bispecific nanobodies, bispecific minibodies, and any genetically manipulated counterparts of the foregoing that retain paratope and target antigen binding function.

[0066] In some embodiments, the immunoconjugate is monovalent. In other embodiments, the immunoconjugate is multivalent, such as, e.g., bivalent. In some further embodiments, the immunoconjugate is bivalent and dimeric. In some further embodiments, the bivalent immunoconjugate is homodimeric.

[0067] In one aspect, the present invention provides antibody constructs (alone or in the context of immunoconjugates, radioimmunoconjugates, or targeted imaging complexes, each of the invention), comprising a VHH fragment comprising a heavy chain variable region comprising three heavy chain CDRs derived from a camelid, which bind to an antigen with specificity and high affinity.

[0068] In some embodiments, the antibody construct, immunoconjugate, radioimmunoconjugate, or targeted imaging complex specifically binds to at least one extracellular part of an antigen expressed on a cellular surface. In some embodiments, the immunoconjugate specifically binds to at least one extracellular part of antigen expressed by a target cell, such as, e.g., a tumor cell.

[0069] In some embodiments, the disclosure provides immunoconjugate that specifically binds to an antigen. In some embodiments, the immunoconjugate comprises an antibody construct comprising a heavy chain variable region (HVR-H) comprising three CDRs: hCDRl, hCDR2, and hCDR3, such as, e.g., derived from a camelid antibody or IgNAR. In some embodiments, the immunoconjugate comprises: (a) a light chain variable region (HVR-L) comprising three CDRs: 1CDR1, 1CDR2, and 1CDR3, and (b) a heavy chain variable region (HVR-H) comprising three CDRs: hCDRl, hCDR2, and hCDR3. In some embodiments, the antibody construct is chimeric or humanized.

[0070] In some embodiments, the immunoconjugate of the present invention comprises an antibody construct comprising an antigen binding domain which is an antibody fragment, including but not limited to, e.g., a Fv, Fab, Fab’, scFv, HcAb fragment, VHH fragment, sdAb fragment, diabody, or F(ab’)2 fragment. In some further embodiments, the immunoconjugate of the present invention comprises a multimer of two or more antibody fragments, such as, e.g., a homodimer or heterodimer comprising two antibody fragments each capable of binding to an antigen with specificity and high affinity and each comprising a heavy chain variable region (HVR-H) comprising three CDRs: hCDRl, hCDR2, and hCDR3.Heavy Chain Constant Regions

[0071] The antigen binding regions of the immunoconjugates described herein may comprise an Fc or heavy chain constant region. The antigen binding molecules can be coupled to the Fc or heavy chain constant region directly, by a suitable linker, or by an IgG hinge region. The inclusion of the heavy chain constant region or Fc region confers such advantages as allowing for optimization and tuning of serum half-life, the addition of additional sites to conjugate a chelating or cytotoxic agent, and allow for purification of the immunoconjugates using standard processes and methods. The addition of a heavy chain constant region also increases the size which may shift the catabolizes and elimination of the immunoconjugate to the liver from the kidney. This can confer safety advantages especially for radioimmunoconjugates as the kidney is more sensitive to radiation than the liver. Alterations that affect the effector function or the serum halflife of can be made to residues present in the heavy chain constant region responsible for binding the neonatal Fc receptor (FcRn). Binding to the FcRn, in general contributes to the increased halflife of molecules that comprise an immunoglobulin Fc, thus reducing binding to FcRn can reduce the half-life of molecules comprising an Fc. Reduction in FcRn binding can confer advantages such as a reduction in the half-life of immunoconjugates, and, thus, subsequent toxicity attributed to cytotoxic agents or radioisotopes. In certain embodiments, the immunoglobulin constant region comprises or consists of an Fc region. In certain embodiments, the immunoglobulin heavy chain constant region comprises a CH2 domain of an immunoglobulin, CH3 domain of an immunoglobulin, or a CH2 and a CH3 domain of an immunoglobulin. In certain embodiments, the immunoglobulin heavy chain constant region comprises a CH2 and a CH3 domain of an immunoglobulin. For treatment or imaging of human individuals the immunoglobulin heavy chain constant region may be human, preventing or reducing an endogenous immune response against the immunoconjugate. In certain embodiments, the immunoglobulin heavy chain constant region is a human immunoglobulin heavy chain constant region. In certain embodiments, the immunoglobulin heavy chain constant region is an IgA, IgGl, IgG2, IgG3, or IgG4 isotype. Incertain embodiments, the immunoglobulin heavy chain constant region is an IgGl isotype. In certain embodiments, the immunoglobulin heavy chain constant region is an IgG4 isotype.

[0072] The immunoglobulin heavy chain constant region can be a variant constant region that comprises one or more alterations to amino acid residue(s) that confers additional utility and advantageous properties to the immunoconjugates described herein. In certain embodiments, the immunoglobulin heavy chain constant region comprises an alteration to one or more amino acid residues that reduces an effector function of the immunoglobulin heavy chain constant region or alters binding of the immunoconjugate to the neonatal Fc receptor (FcRn).In certain embodiments, the immunoglobulin heavy chain constant region comprises an alteration to one or more amino acid residues that reduces an effector function of the immunoglobulin heavy chain constant region or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn). In certain embodiments, the immunoglobulin heavy chain constant region comprises an alteration to one or more amino acid residues that reduces an effector function of the immunoglobulin heavy chain constant region and reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn). In certain embodiments, the immunoglobulin heavy chain constant region comprises an alteration to one or more amino acid residues that reduces an effector function of the immunoglobulin heavy chain constant region. In certain embodiments, the immunoglobulin heavy chain constant region comprises an alteration to one or more amino acid residues that reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn).

[0073] The alterations to heavy chain constant regions of the immunoconjugate can reduce effector function associated with a heavy chain constant region, such as, the ability to fix complement, promote phagocytosis, or recruit other immune effector cells (e.g., NK cells) to the heavy chain constant region. In certain embodiments, the alteration to one or more amino acid residues that reduces the effector function of the immunoglobulin heavy chain constant region is an alteration that reduces complement dependent cytotoxicity (CDC), antibody-dependent cellcytotoxicity (ADCC), antibody-dependent cell-phagocytosis ADCP, or a combination thereof. In certain embodiments, the alteration to one or more amino acid residues that reduces the effector function of the immunoglobulin heavy chain constant region is selected from the list consisting of: (a) 297A, 297Q, 297G, or 297D, (b) 279F, 279K, or 279L, (c) 228P, (d) 235 A, 235E, 235G, 235Q, 235R, or 235S, (e) 237A, 237E, 237K, 237N, or 237R, (f) 234A, 234V, or 234F, (g) 233P, (h) 328A, (i) 327Q or 327T, (j) 329A, 329G, 329Y, or 329R (k) 331 S, (1) 236F or 236R, (m) 238 A, 238E, 238G, 238H, 2381, 238V, 238W, or 238 Y, (n) 248 A, (o) 254D, 254E, 254G, 254H, 2541, 254N, 254P, 254Q, 254T, or 254V, (p) 255N, (q) 256H, 256K, 256R, or 256V, (r) 264S, (s) 265H, 265K, 265 S, 265 Y, or 265 A, (t) 267G, 267H, 2671, or 267K, (u) 268K, (v) 269N or 269Q, (w)270 A, 270G, 270M, or 270N, (x) 27 IT, (y) 272N, (z) 292E, 292F, 292G, or 2921, (aa) 293 S, (bb) 301W, (cc) 304E, (dd) 311E, 311G, or 31 IS, (ee) 316F, (ff) 328V, (gg) 330R, (hh) 339E or 339L, (ii) 3431 or 343V, (jj) 373 A, 373G, or 373S, (kk) 376E, 376W, or 376Y, (11) 380D, (mm) 382D or 382P, (nn) 385P, (oo) 424H, 424M, or 424V, (pp) 4341, (qq) 438G, (rr) 439E, 439H, or 439Q, (ss) 440A, 440D, 440E, 440F, 440M, 440T, or 440V, (tt) K322A, (uu) L235E, (vv) L234A and L235A, (ww) L234A, L235A, and G237A, (xx) L234A, L235A, and P329G, (yy) L234F, L235E, and P331S, (zz) L234A, L235E, and G237A, (aaa), L234A, L235E, G237A, and P331S (bbb) L234A, L235A, G237A, P238S, H268A, A330S, and P331S, (ccc) L234A, L235A, and P329A, (ddd) G236R and L328R, (eee) G237A, (fff) F241A, (ggg) V264A, (hhh) D265A, (iii) D265A and N297A, (jjj) D265A and N297G, (kkk) D270A, (111) A330L, (mmm) P331A or P331S, or (nnn) E233P, (ooo) L234A, L235E, G237A, A330S, and P331S or (ppp) any combination of (a) - (ooo), per EU numbering. In certain embodiments, the alteration to one or more amino acid residues that reduces the effector function of the immunoglobulin heavy chain constant region comprises L234A, L235E, G237A, A330S, and P331S per EU numbering.

[0074] The alterations to heavy chain constant regions of the immunoconjugate can reduce the serum half-life of the immunoconjugate. In certain embodiments, the amino acid alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) reduces the serum half-life of the immunoconjugate. In certain embodiments, the alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) is to an amino acid residue selected from the list consisting of: 251, 252, 253, 254, 255, 288, 309, 310, 312, 385, 386, 388, 400, 415, 433, 435, 436, 439, 447, and combinations thereof per EU numbering. In certain embodiments, the alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) is to an amino acid residue selected from the list consisting of: 253, 254, 310, 435, 436 and combinations thereof per EU numbering . In certain embodiments, the alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) is to an amino acid residue selected from the list consisting of: 1253 A, I253D, I253P, S254A, H310A, H310D, H310E, H310Q, H435A, H435Q, Y436A, and combinations thereof per EU numbering. In certain embodiments, the alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) is to an amino acid residue selected from the list consisting of: 1253 A, S254A, H310A, H435Q, Y436A and combinations thereof per EU numbering. In certain embodiments, the alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) is to an amino acid residue selected from the list consisting of: 1253 A, H310A, H435Q, and combinations thereof per EU numbering. In certain embodiments, the alteration that alters or reduces binding of the immunoconjugate to the neonatal Fc receptor (FcRn) is to anamino acid residue selected from the list consisting of: H310A, H435Q, and combinations thereof per EU numbering.

[0075] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 1. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 1. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 1, wherein the heavy chain constant region comprises an 1253 A substitution per EU numbering.

[0076] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 2. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 2. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 2, wherein the heavy chain constant region comprises an S254A substitution per EU numbering.

[0077] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 3. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 3. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 3, wherein the heavy chain constant region comprises an H310A substitution per EU numbering.

[0078] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 4. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 4. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 4, wherein the heavy chain constant region comprises an H435Q substitution per EU numbering.

[0079] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 5. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 5. In certain embodiments, a heavy chain constantregions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 5, wherein the heavy chain constant region comprises an Y436A substitution per EU numbering.

[0080] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 6. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 6. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 6, wherein the heavy chain constant region comprises an H310A / H435Q substitution per EU numbering.

[0081] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 7. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 7. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 7, wherein the heavy chain constant region comprises a L234A, L235E, G237A, A330S, and P331 S substitution per EU numbering.

[0082] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 8. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 8, wherein the heavy chain constant region comprises a L234A, L235E, G237A, H310A, A330S, and P331 S substitution per EU numbering.

[0083] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 9. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 9. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 9, wherein the heavy chain constant region comprises a L234A, L235E, G237A, H435Q, A330S, and P331S substitution per EU numbering.

[0084] In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence at least 90%, 95%, 97%, 98%, or 99% identical to the sequence set forth in SEQ ID NO: 10. In certain embodiments, a heavy chain constant regions of the immunoconjugate comprises a sequence identical to SEQ ID NO: 10 per EU numbering.

[0085] In one embodiment, each of the two variant constant regions has at least one FcRn binding mutation. In one embodiment, each of the two variant constant regions has the same FcRn binding mutation. In one embodiment, each of the two variant constant regions has a different FcRn binding mutation.

[0086] In one embodiment, at least one of the variant constant regions in the immunoconjugate has at least one FcRn binding mutation. In a preferred embodiment, each of the two variant constant regions of the immunoconjugate has at least one FcRn binding mutation, which FcRn binding mutations are the same or different.

[0087] Alterations that effect FcRn binding can reduce the serum half-life of the immunoconjugate, thus allowing the skilled artisan to choose a half-life that is suitable for a particular imaging or therapeutic goal. In certain embodiments, the immunoconjugate has a serum half-life of about 12 hours to about 120 hours. In certain embodiments, the immunoconjugate has a serum half-life of about 12 hours to about 24 hours, about 12 hours to about 36 hours, about 12 hours to about 48 hours, about 12 hours to about 60 hours, about 12 hours to about 72 hours, about 12 hours to about 84 hours, about 12 hours to about 96 hours, about 12 hours to about 108 hours, about 12 hours to about 120 hours, about 24 hours to about 36 hours, about 24 hours to about 48 hours, about 24 hours to about 60 hours, about 24 hours to about 72 hours, about 24 hours to about 84 hours, about 24 hours to about 96 hours, about 24 hours to about 108 hours, about 24 hours to about 120 hours, about 36 hours to about 48 hours, about 36 hours to about 60 hours, about 36 hours to about 72 hours, about 36 hours to about 84 hours, about 36 hours to about 96 hours, about 36 hours to about 108 hours, about 36 hours to about 120 hours, about 48 hours to about 60 hours, about 48 hours to about 72 hours, about 48 hours to about 84 hours, about 48 hours to about 96 hours, about 48 hours to about 108 hours, about 48 hours to about 120 hours, about 60 hours to about 72 hours, about 60 hours to about 84 hours, about 60 hours to about 96 hours, about 60 hours to about 108 hours, about 60 hours to about 120 hours, about 72 hours to about 84 hours, about 72 hours to about 96 hours, about 72 hours to about 108 hours, about 72 hours to about 120 hours, about 84 hours to about 96 hours, about 84 hours to about 108 hours, about 84 hours to about 120 hours, about 96 hours to about 108 hours, about 96 hours to about 120 hours, or about 108 hours to about 120 hours. In certain embodiments, the immunoconjugate has a serum halflife of about 12 hours, about 24 hours, about 36 hours, about 48 hours, about 60 hours, about 72 hours, about 84 hours, about 96 hours, about 108 hours, or about 120 hours. In certain embodiments, the immunoconjugate has a serum half-life of at least about 12 hours, about 24 hours, about 36 hours, about 48 hours, about 60 hours, about 72 hours, about 84 hours, about 96 hours, or about 108 hours. In certain embodiments, the immunoconjugate has a serum half-life ofT1at most about 24 hours, about 36 hours, about 48 hours, about 60 hours, about 72 hours, about 84 hours, about 96 hours, about 108 hours, or about 120 hours.

[0088] In certain embodiments, the immunoconjugate has a serum half-life of about 1 day to about 10 days. In certain embodiments, the immunoconjugate has a serum half-life of about 1 day to about 2 days, about 1 day to about 3 days, about 1 day to about 4 days, about 1 day to about 5 days, about 1 day to about 6 days, about 1 day to about 7 days, about 1 day to about 8 days, about 1 day to about 9 days, about 1 day to about 10 days, about 2 days to about 3 days, about 2 days to about 4 days, about 2 days to about 5 days, about 2 days to about 6 days, about 2 days to about 7 days, about 2 days to about 8 days, about 2 days to about 9 days, about 2 days to about 10 days, about 3 days to about 4 days, about 3 days to about 5 days, about 3 days to about 6 days, about 3 days to about 7 days, about 3 days to about 8 days, about 3 days to about 9 days, about 3 days to about 10 days, about 4 days to about 5 days, about 4 days to about 6 days, about 4 days to about7 days, about 4 days to about 8 days, about 4 days to about 9 days, about 4 days to about 10 days, about 5 days to about 6 days, about 5 days to about 7 days, about 5 days to about 8 days, about 5 days to about 9 days, about 5 days to about 10 days, about 6 days to about 7 days, about 6 days to about 8 days, about 6 days to about 9 days, about 6 days to about 10 days, about 7 days to about8 days, about 7 days to about 9 days, about 7 days to about 10 days, about 8 days to about 9 days, about 8 days to about 10 days, or about 9 days to about 10 days. In certain embodiments, the immunoconjugate has a serum half-life of about 1 day, about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, about 9 days, or about 10 days. In certain embodiments, the immunoconjugate has a serum half-life of at least about 1 day, about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, or about 9 days. In certain embodiments, the immunoconjugate has a serum half-life of at most about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, about 9 days, or about 10 days.

[0089] In certain embodiments, the heavy chain constant region has a molecular weight of about 10 kDa to about 25 kDa. In certain embodiments, the heavy chain constant region has a molecular weight of about 10 kDa to about 15 kDa, about 10 kDa to about 20 kDa, about 10 kDa to about 25 kDa, about 15 kDa to about 20 kDa, about 15 kDa to about 25 kDa, or about 20 kDa to about 25 kDa. In certain embodiments, the heavy chain constant region has a molecular weight of about 10 kDa, about 15 kDa, about 20 kDa, or about 25 kDa. In certain embodiments, the heavy chain constant region has a molecular weight of at least about 10 kDa, about 15 kDa, or about 20 kDa. In certain embodiments, the heavy chain constant region has a molecular weight of at most about 15 kDa, about 20 kDa, or about 25 kDa.

[0090] In some embodiments, the immunoconjugate of the present invention comprises a linker or hinge region, which is a polypeptide linking an antigen binding region to a heavy chain constant region or a variant constant region in the instant invention. Naturally occurring and synthetic hinge regions linking immunoglobulin components are well known in the art and available for use in the present invention. For example, see US 8,067,548 and references therein.

[0091] In one embodiment, the hinge regions of the immunoconjugate are the same. In one embodiment, the hinge regions of the immunoconjugate are different.

[0092] The antigen binding regions and the heavy chain constant regions (with or without an altered amino acid sequence) can be connected by a suitable hinge or linker sequence. In certain embodiments, the antigen binding region is coupled to the immunoglobulin heavy chain constant region by a linker amino acid sequence or a human IgG hinge region. Appropriate IgG hinge regions comprise and include IgGl or IgG4 hinge regions. In certain embodiments, the hinge region is an IgGl hinge region. In certain embodiments, the hinge region is an IgGl hinge regions with a with a C220S substitution per EU numbering. Suitable hinge regions include those described in Wu et al., “Multimerization of a chimeric anti-CD20 single-chain Fv-Fc fusion protein is mediated through variable domain exchange,” Protein Engineering, Design and Selection, Volume 14, Issue 12, December 2001, Pages 1025-1033; Shu et al, “Secretion of a single-gene-encoded immunoglobulin from myeloma cells.” Proceedings of the National Academy of Sciences Sep 1993, 90 (17) 7995-7999; Davis et al., “Abatacept binds to the Fc receptor CD64 but does not mediate complement-dependent cytotoxicity or antibody -dep endent cellular cytotoxicity.” J Rheumatol. 2007 Nov;34(l l):2204-10. Appropriate hinges may also include a non-IgG based polypeptide linker. The linker amino acid sequence may predominantly include the following amino acid residues: Gly, Ser, Ala, or Thr. The linker peptide should have a length that is adequate to link two molecules in such a way that they assume the correct conformation relative to one another, and so that they retain the desired activity. In one embodiment, the linker is from about 1 to 50 amino acids in length or about 1 to 30 amino acids in length. In one embodiment, linkers of 1 to 20 amino acids in length may be used. Useful linkers include glycine-serine polymers, including for example (GS)n, (GSGGS)n, (GGGGS)n, and (GGGS)n, where n is an integer of at least one, glycine-alanine polymers, alanine-serine polymers, and other flexible linkers. Exemplary, linkers for linking antibody fragments or single chain variable fragments can include AAEPKSS (SEQ ID NO: 256), AAEPKSSDKTHTCPPCP (SEQ ID NO: 257), GGGG (SEQ ID NO: 258), or GGGGDKTHTCPPCP (SEQ ID NO: 259). Alternatively, a variety of non-proteinaceous polymers, including but not limited to polyethyleneglycol (PEG), polypropylene glycol, polyoxy alkylenes, or copolymers of polyethylene glycol and polypropylene glycol, may find use as linkers, that is may find use as linkers.

[0093] The total size of the immunoconjugate may be such that it promotes tissue penetration, stability, and / or clearance. In certain embodiments, the immunoconjugate has a molecular weight of about 60 kDa to about 120 kDa. In certain embodiments, the immunoconjugate has a molecular weight of about 60 kDa to about 65 kDa, about 60 kDa to about 70 kDa, about 60 kDa to about 75 kDa, about 60 kDa to about 80 kDa, about 60 kDa to about 90 kDa, about 60 kDa to about 100 kDa, about 60 kDa to about 110 kDa, about 60 kDa to about 120 kDa, about 65 kDa to about 70 kDa, about 65 kDa to about 75 kDa, about 65 kDa to about 80 kDa, about 65 kDa to about 90 kDa, about 65 kDa to about 100 kDa, about 65 kDa to about 110 kDa, about 65 kDa to about 120 kDa, about 70 kDa to about 75 kDa, about 70 kDa to about 80 kDa, about 70 kDa to about 90 kDa, about 70 kDa to about 100 kDa, about 70 kDa to about 110 kDa, about 70 kDa to about 120 kDa, about 75 kDa to about 80 kDa, about 75 kDa to about 90 kDa, about 75 kDa to about 100 kDa, about 75 kDa to about 110 kDa, about 75 kDa to about 120 kDa, about 80 kDa to about 90 kDa, about 80 kDa to about 100 kDa, about 80 kDa to about 110 kDa, about 80 kDa to about 120 kDa, about 90 kDa to about 100 kDa, about 90 kDa to about 110 kDa, about 90 kDa to about 120 kDa, about 100 kDa to about 110 kDa, about 100 kDa to about 120 kDa, or about 110 kDa to about 120 kDa. In certain embodiments, the immunoconjugate has a molecular weight of about 60 kDa, about 65 kDa, about 70 kDa, about 75 kDa, about 80 kDa, about 90 kDa, about 100 kDa, about 110 kDa, or about 120 kDa. In certain embodiments, the immunoconjugate has a molecular weight of at least about 60 kDa, about 65 kDa, about 70 kDa, about 75 kDa, about 80 kDa, about 90 kDa, about 100 kDa, or about 110 kDa. In certain embodiments, the immunoconjugate has a molecular weight of at most about 65 kDa, about 70 kDa, about 75 kDa, about 80 kDa, about 90 kDa, about 100 kDa, about 110 kDa, or about 120 kDa.

[0094] In some embodiments, the immunoconjugate has a molecular weight greater than 60, 70, 75, 80, 82, 83, 85, 86, 87, 88 or 89 kDa. In some embodiments, the immunoconjugate has a molecular weight less than 110, 100, 95, 93, 91, 90, 89, 88, 87, 86, 85, 84, 83, 82, 81, or 80 kDa. In some embodiments, the immunoconjugate has a molecular weight greater than 60, 65, 70, 71, 72, 73, 74, 75, 76, 77, 78, or 79 kDa and less than 110, 100, 95, 93, 91, or 90 kDa.

[0095] The sizes of the immunoconjugates and / or the heavy chain constant region variants described herein allow for an increased safety profile or therapeutic index of the immunoconjugates included herein. Such a safety profile may be reflected in the reduction of accumulation of radiation in radio sensitive major tissues such as kidney and bone marrow and / oran increase in radiation accumulation in target tissues (i.e., a tumor or cancerous tissue) or more radio tolerant organs such as the liver.

[0096] In certain embodiments, the immunoconjugates of this disclosure result in a total radiation exposure per treatment as measured in Gray (Gy). In certain embodiments, the kidney is exposed to 20 Gy or less per treatment. In certain embodiments, the kidney is exposed to 19 Gy or less per treatment. In certain embodiments, the kidney is exposed to 18 Gy or less per treatment. In certain embodiments, the kidney is exposed to 17 Gy or less per treatment. In certain embodiments, the kidney is exposed to 16 Gy or less per treatment. In certain embodiments, the kidney is exposed to 15 Gy or less per treatment. In certain embodiments, the kidney is exposed to 14 Gy or less per treatment. In certain embodiments, the kidney is exposed to 13 Gy or less per treatment. In certain embodiments, the kidney is exposed to 12 Gy or less per treatment. In certain embodiments, the kidney is exposed to 11 Gy or less per treatment. In certain embodiments, the kidney is exposed to 10 Gy or less per treatment. In certain embodiments, the kidney is exposed to 9 Gy or less per treatment. In certain embodiments, the kidney is exposed to 8 Gy or less per treatment. In certain embodiments, the kidney is exposed to 5 Gy or less per treatment.

[0097] In certain embodiments, the immunoconjugates of this disclosure result in a total radiation exposure per treatment as measured in Gray (Gy). In certain embodiments, the bone marrow is exposed to 4 Gy or less per treatment. In certain embodiments, the bone marrow is exposed to 3 Gy or less per treatment. In certain embodiments, the bone marrow is exposed to 2 Gy or less per treatment. In certain embodiments, the bone marrow is exposed to 1.5 Gy or less per treatment. In certain embodiments, the bone marrow is exposed to 1.0 Gy or less per treatment. In certain embodiments, the bone marrow is exposed to 0.5 Gy or less per treatment.

[0098] In certain embodiments, the immunoconjugates of this disclosure result in an increased amount of radiation in the tumor compared to the kidney when measured as a percent injected dose per gram. In certain embodiments, the ratio of tumor percent injected dose per gram to kidney percent injected dose per gram is greater than 2: 1, 3: 1, 4: 1, 5: 1, 6: 1, 7: 1, 8: 1, 9: 1, or 10: 1.

[0099] In certain embodiments, the immunoconjugates of this disclosure result in an increased amount of radiation in the tumor compared to the blood when measured as percent injected dose per gram. In certain embodiments, the ratio of tumor percent injected dose per gram to blood percent injected dose per gram is greater than 2: 1, 3: 1, 4: 1, 5: 1, 6: 1, 7: 1, 8: 1, 9: 1, or 10: 1.

[0100] In certain embodiments, the immunoconjugates of this disclosure result in an increased amount of radiation in the tumor compared to the bone marrow when measured as percent injected dose per gram. In certain embodiments, the ratio of tumor percent injected dose per gram to bone marrow percent injected dose per gram is greater than 2: 1, 3: 1, 4: 1, 5: 1, 6: 1, 7: 1, 8: 1, 9: 1, or 10: 1.

[0101] In certain embodiments, the immunoconjugates of this disclosure result in an increased amount of radiation in the liver compared to the kidney when measured as an injected dose per gram. In certain embodiments, the ratio of tumor percent injected dose per gram to bone marrow percent injected dose per gram is greater than 3: 1, 4: 1, 5: 1, 6: 1, 7: 1, 8: 1, 9: 1, or 10: 1.

[0102] In some embodiments, the invention contemplates a variant of an immunoconjugate of the invention that comprises a Fc region wherein the variant possesses some but not all effector functions, which make it a desirable candidate for applications in which the half-life of the immunoconjugate in vivo is important yet certain effector functions (such as complement and ADCC) are unnecessary or deleterious. In vitro and / or in vivo cytotoxicity assays can be conducted to confirm the reduction / depletion of CDC and / or ADCC activities. For example, Fc receptor (FcR) binding assays can be conducted to ensure that the immunoconjugate lacks FcyyR binding (hence likely lacking ADCC activity), but retains FcRn binding ability. The primary cells for mediating ADCC, NK cells, express FcyyRIII only, whereas monocytes express FcyyRI, FcyyRII and FcyRIII. FcR expression on hematopoietic cells is summarized in Table 3 on page 464 of Ravetch and Kinet, Annu. Rev. Immunol. 9:457-492 (1991). Non-limiting examples of in vitro assays to assess ADCC activity of a molecule of interest is described in US 5,500,362 (see e.g. Hellstrom, I . et al. Proc Natl Acad Sci USA 83:7059-7063 (1986)) and Hellstrom, I et al., Proc Natl Acad Sci USA 82: 1499-1502 (1985); 5,821,337 (see Bruggemann, M. et al., J. Exp. Med. 166: 1351-1361 (1987)). Alternatively, non-radioactive assays methods may be employed (see, for example, ACTI™ non-radioactive cytotoxicity assay for flow cytometry (CellTechnology, Inc. Mountain View, CA; and CytoTox 96® non-radioactive cytotoxicity assay (Promega, Madison, WI). Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and Natural Killer (NK) cells. Alternatively, or additionally, ADCC activity of the molecule of interest may be assessed in vivo, e.g., in an animal model such as that disclosed in Clynes et al. Proc Natl Acad Sci USA 95:652-656 (1998). Clq binding assays may also be carried out to confirm that the immunoconjugate is unable to bind Clq and hence lacks CDC activity (see e.g., Clq and C3c binding ELISA in WO 2006 / 029879 and WO 2005 / 100402). To assess complement activation, a CDC assay may be performed (see e.g., Gazzano-Santoro etal., J. Immunol. Methods 202: 163 (1996); Cragg, M.S. et al., Btoo 101 : 1045-1052 (2003); Cragg, M.S. and M.J. Glennie, Blood 103:2738-2743 (2004)). FcRn binding and in vivo clearance / half-life determinations can also be performed using methods known in the art (see e.g., Petkova, S.B. et al., Int'l. Immunol. 18(12): 1759-1769 (2006)).

[0103] Immunoconjugates with reduced effector function include those with substitution of one or more of Fc region residues 238, 265, 269, 270, 297, 327, and 329 (US 6,737,056). Such Fcmutants include Fc mutants with substitutions at two or more of amino acid positions 265, 269, 270, 297 and 327, including the so-called “DANA” Fc mutant with substitution of residues 265 and 297 to alanine (US 7,332,581).

[0104] The immunoconjugate may have altered effector function by comprising the following alterations L234A, L235E, G237A, A330S, and P331S per EU numbering, which reduce Fc receptor binding. See e.g., US 8,613,926 or Andersson C, Wenander et al., ’’Rapid-onset clinical and mechanistic effects of anti-C5aR treatment in the mouse collagen-induced arthritis model.” Clin Exp Immunol. 2014 Jul; 177(1):219-33.

[0105] Certain immunoconjugate variants with improved or diminished binding to FcRs are described (see e.g., US 6,737,056; WO 2004 / 056312; Shields et al., J. Biol. Chem. 9(2): 6591- 6604 (2001)).

[0106] In some embodiments, alterations are made in the Fc region that result in altered (i.e., either improved or diminished) Clq binding and / or Complement Dependent Cytotoxicity (CDC), e.g., as described in US 6,194,551; WO 1999 / 051642; Idusogie et al. J. Immunol. 164: 4178-4184 (2000).

[0107] Antibodies with increased half-lives and improved binding to the neonatal Fc receptor (FcRn), which is responsible for the transfer of maternal IgGs to the fetus (Guyer et al., J. Immunol. 117:587 (1976); Kim et al., J. Immunol. 24:249 (1994)), are described in US2005 / 0014934. Those antibodies comprise an Fc region with one or more substitutions therein which improve binding of the Fc region to FcRn. Such Fc variants include those with substitutions at one or more of Fc region residues: 434 or 435, e.g., substitution of Fc region residue N434A or R435A (US 7,371,826). See also Duncan and Winter, Nature 322:738-40 (1988); US 5,648,260; US 5,624,821; and WO 1994 / 029351 concerning other examples of Fc region variants.

[0108] To increase the serum half-life of the antibody, one may incorporate a salvage receptor binding epitope into the antibody (especially an antibody fragment) as described in U.S. Patent 5,739,277, for example. As used herein, the term “salvage receptor binding epitope” refers to an epitope of the Fc region of an IgG molecule (e.g., IgGl, IgG2, IgG3, or IgG4) that is responsible for increasing the in vivo serum half-life of the IgG molecule.

[0109] As will be recognized by the person of ordinary skill in the art, certain teachings herein apply to antibody constructs, targeted imaging complexes, immunoconjugates and radioimmunoconjugates of the invention, notwithstanding that reference is made in the text to one only or two such compositions (e.g., immunoconjugate) as a non-limiting example. All such applications and embraced by the present invention.Chelating agents

[0110] As described herein, in some embodiments a chelating agent (i.e., R1) is coupled to the tumor targeting moiety (e.g., the polypeptides comprising an antigen binding region and an immunoglobulin heavy chain constant region described herein). The chelating moiety allows for the tumor targeting moieties to be loaded with an appropriate radioisotope, such as a beta emitter or an alpha emitter. The chelator can be coupled to the antigen binding region, the heavy chain constant region, the immunoglobulin Fc region, or any combination thereof. Such coupling can suitably be by a covalent attachment to one or more amino acids of the immunoconjugate, the antigen binding region, the heavy chain constant region, the immunoglobulin Fc region, or any combination thereof.

[0111] In some embodiments, the chelator is conjugated at a predefined ratio of polypeptide (i.e., antigen binding region and / or the immunoglobulin heavy chain constant) to chelator. In certain embodiments, the radioisotope chelating agent is coupled to the antigen binding region and / or the immunoglobulin heavy chain constant region at a ratio of 1 : 1 to 8:1. In certain embodiments, the radioisotope chelating agent is coupled to the antigen binding region and / or the immunoglobulin heavy chain constant region at a ratio of 1 : 1 to 6: 1. In certain embodiments, the radioisotope chelating agent is coupled to the antigen binding region and / or the immunoglobulin heavy chain constant region at a ratio of 2: 1 to 6: 1.

[0112] In one embodiment, the radioisotope chelating component comprises DOTA or a DOTA derivative. In one embodiment, the radioisotope chelating component comprises DOTAGA. In one embodiment, the radioisotope chelating component comprises macropa or a macropa derivative. In one embodiment, the radioisotope chelating component comprises Py4Pa or a Py4Pa derivative.

[0113] In one embodiment, the chelating agent comprises DOTA or a DOTA derivative. In one embodiment, the chelating agent comprises DOTAGA. In one embodiment, the chelating agent comprises macropa or a macropa derivative. In one embodiment, the chelating agent comprises Py4Pa or a Py4Pa derivative. In one embodiment, the chelating agent comprises siderocalin or a siderocalin derivative.

[0114] In certain embodiments, the chelating agent is a radioisotope chelating agent. In certain embodiments, the radioisotope chelating agent is selected from the list consisting of: tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTA), a-(2-Carboxyethyl)-l,4,7,10- tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTAGA), or (Py4Pa). In certain embodiments, the radioisotope chelating agent is DOTA. In certain embodiments, the radioisotope chelating agent is DOTAGA. In certain embodiments, the radioisotope chelating agent is Py4Pa.

[0115] Examples of chelators are l,4,7,10-tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTA), diethylene triamine pentaacetic acid (DTP A), and related analogs of the aforementioned. Such chelators are suitable for coordinating metal ions like a and P-emitting radionuclides.

[0116] In some embodiments the chelating agent of an immunoconjugate or radioimmunoconjugate of the invention is selected from the group comprising bifunctional chelator, DOTA, DO3A, DOTAGA, Bn-DOTA, Bn-DTPA, Bn-CHX-A”-DTPA, Bn-TCMC, macropa (Thiele NA, et al. Angew. Chem. Int. Ed. 56: 1 (2017)), crown (Yang H, et al. Chem. Eur. J. 26: 11435 (2020)), P-SCN-Ph-Et-Py4Pa (Li L, et al. Bioconjugate Chem. ASAP (2020)), 3,2-HOPO (Wickstroem K, et al. Int. J. Rad. One. Biol. Phys. 105:410 (2019)) (For a review of these and other bifunctional chelators See e.g., Price EW and Orvig C Chem. Soc. Rev., 2014, 43:260 (2014) and Brechbiel MW Q. J. Nucl. Med. Mol. Imaging 52:166 (2008)).

[0117] For225Ac immunoconjugates, there are a variety of acyclic and cyclic ligands known in the art as suitable chelators (see e.g., Davis I, et al., Nucl Med Biol 26: 581 (1999); Chappell L, et al., Bioconjug Chem 11 : 510 (2000); Chappell, L, et al., Nucl Med Biol 30: 581 (2003); McDevitt M, et al., Appl Radiat Isot 57: 841 (2002); Gouin S, et al., Org Biomol Chem 3: 453 (2005); Thiele N, et al., Angew Chem Int Ed Engl 56: 14712 (2017)).

[0118] In certain embodiments, the chelator is a chelator suitable for alpha emitter chelation. Some chelators suitable for alpha emitters are described in Yang et al, “Harnessing a-Emitting Radionuclides for Therapy: Radiolabeling Method Review.” J Nucl Med. 2022 Jan;63(l):5-13.

[0119] In certain embodiments the, chelator suitable for alpha emitter chelation is selected from the list consisting of: l,4,7,10-tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTA); 1 ,4,7-Tris(carboxymethyl)-l ,4,7, 10-tetraazacyclododecane (DO3 A); a-(2-Carboxyethyl)- 1,4,7, 10-tetraazacyclododecane-l, 4,7, 10-tetraacet c acid (DOTAGA); 6,6',6",6'"-(((pyridine-2,6- diylbis(methylene))bis(azanetriyl))-tetrakis(methylene))tetrapicolinic acid (Py4Pa); 2,2' ,2" ,2'"- (1,10-dioxa-4,7, 13,16-tetraazacyclo-octadecane-4,7, 13,16-tetrayl)tetraacetic acid (Crown); 6,6'- ((1,4,10,13 -tetraoxa-7, 16-diazacyclooctadecane-7, 16-diyl)bis(methylene))-dipicolinic acid (Macropa); 1,4,7,10,13,16-hexaazacyclohexadecane- 1,4,7,10,13,16-hexaacetic acid(HEHA); 6,6'-[(lR,2R)-l,2-Cyclohexanediylbis[[(carboxymethyl)imino]methylene]]bis[2- pyridinecarboxylic acid] (CHXoctapa); 3,7-Diazabicyclo[3.3.1]nonane-l,5-dicarboxylic acid, 7- [(6-carboxy-2-pyridinyl)methyl]-9-hydroxy-3-methyl-2,4-di-2-pyridinyl-,l,5-dimethyl ester (Bispa); 6,6'-(((oxybis(ethane-2, 1 -diyl))bis((carboxymethyl)azanediyl))bis(methylene))- dipicolinic acid (Noneunpa); and combinations thereof.

[0120] In certain embodiments, the chelator is a chelator suitable for an beta- or gammaemitter chelation. In certain embodiments the, chelator suitable for an beta- or gamma-emitterchelation is selected from the list consisting of: (lR,4R,7R,10R)-a,a',a",a"'-tetramethyl-l,4,7,10- tetraaza-cyclododecane-l,4,7,10-tetraacetic acid (DOTMA); (1,4,7,10- tetrakis(carbamoylmethyl)-l,4,7, 10-tetraazacyclododecane) (DOTAM); 1,4,7, 10-tetraazacyclo- dodecane-l,4,7,10-tetrapropionic acid (DOTPA); (2-(4,7,10-tris(2-amino-2-oxoethyl)-l,4,7,10- tetraazacyclo-dodecan-l-yl)acetic acid) (DO3 AM-acetic acid); 1,4,7,10- tetraazacyclododecane- 1 ,4,7, 10-tetra(m ethylene phosphonic acid) (DOTP); 1,4, 6,10-tetraazacyclodecane- 1,4, 7,10- tetramethylene phosphonic acid (DOTMP); 1,4,7, 10-tetraazacy clododecane- 1,4, 7,10- tetrakis(acetamido-methylenephosphonic acid) (DOTA-4AMP); (1,4,8,11- tetraazabicy clo[6.6.2]hexa-decane-4, 11 -diacetic acid) (CB-TE2A); 1,4,7-triaza-cyclononane-l ,4,7-triacetic acid (NOTA); 1,4,7-triazacyclononane-l , 4, 7-tri (methylene phosphonic acid) (NOTP); l,4,8,l l-tetraazacyclo-tetradecane-l,4,8,l l-tetrapropionic acid (TETPA); 1,4,8,11- tetraazacy cl otetradecane- 1,4, 8, 11 -tetraacetic acid (TETA); 1,4,7,10,13- pentaazacyclopentadecane-N,N',N",N"',N""-pentaacetic acid (PEPA); N,N'-bis(6-carboxy-2- pyridylmethyl)-ethylenediamine-N,N'-diacetic acid (H4Octapa); l,2-[[6-(carboxy)-pyridin-2-yl]- methylamino]ethane (H2Dedpa); N,N'- (methylenephosphonate)-N,N'-[6- (methoxycarbonyl)pyridin-2-yl]-methyl-l,2-diaminoethane (H6phospa); triethylene-tetramine- N,N,N',N",N"',N" '-hexaacetic acid (TTHA); tetraazacyclo-dodecane dimethane-phosphonic acid (DO2P); hydroxypropyltetraazacyclododecanetriacetic acid (HP-DO3A); ethylenediaminetetraacetic acid (EDTA); diethylenetriaminepentaacetic acid (DTPA); diethylenetriaminepentaacetic acid-bismethylamide (DTPA-BMA); octadentate hy droxypyridinones (HOPO); N,N' -(butane- 1 , 4 - diy 1 )b i s( 1 -hydroxy -N -(3 -( 1 -hy droxy-6-oxo- 1,6- dihydro-pyridine-2-carboxamido)propyl)-6-oxo-l,6-dihydropyridine-2-carboxamide) (3,2,3- LI(HOPO)); N,N'-(((2-(4-aminobenzyl)-3-((2-(3-hydroxy-l-methyl-2-oxo-l,2-dihydropyridine- 4-carboxamido)ethyl)(2-(3-hydroxy-2-oxo-l,2-dihydropyridine-4-carboxamido)ethyl)amino)- propyl)azanediyl)bis(ethane-2, 1 -diy l))bi s(3 -hydroxy- 1 -methyl-2-oxo- 1 ,2-dihydropyridine-4- carboxamide) (3,2-HOPO); 6,6'-(((azanediylbis(ethane-2, l-diyl))bis((carboxymethyl)- azanediyl))bis(methylene))dipicolinic acid (neunpa); 6,6'-((ethane-l,2-diylbis((carboxymethyl)- azanediyl))bis(methylene))dipicolinic acid (octapa); 2,2'-(ethane-l,2-diylbis(((8-hydroxy- quinolin-2-yl)methyl)azanediyl))diacetic acid (octox); 6,6'-(((pyridine-2,6-diylbis(methylene))- bis((carboxymethyl)azanediyl))-bis(methylene))-dipicolinic acid (PyPa); 21,22,23,24-tetraaza- pentacyclo[16.2.1.13,6.18,11.113,16]tetracosa-l,3,5,7,9,l l(23), 12,14,16,18(21),19-undecaene (porphyrin); 30-amino-3,14,25-trihydroxy3,9,14,20, 25-pentaazatriacontane-2,10,13,21,24- pentaone (deferoxamine); Nl-[5-(Acetylhydroxyamino)-pentyl]-N26-(5-aminopentyl)-N26,5,16-trihydroxy-4, 12, 15, 23-tetraoxo-5,l 1,16, 22-tetraazahexacosane-diamide (DFO*); and combinations thereof.

[0121] In some embodiments, R1comprises a chelating moiety selected from the list consisting of: DOTA, DO3A, DO3Apic, DOTAGA, Py4Pa, Py4Pa-NCS, Crown, Macropa, HEHA, CHXoctapa, Bispa, and Noneunpa; or a radionuclide complex thereof.

[0122] In some embodiments, R1comprises a chelating moiety selected from the list consisting of: DOTMA, DOTPA, DO3Apic, DO3AM-acetic acid, DOTP, DOTMP, DOTA- 4AMP, CB-TE2A, NOTA, NOTP, TETPA, TETA, PEPA, H40ctapa, H2Dedpa, DO2P, EDTA, DTPA-BMA, 3,2,3 -LI(HOPO), 3,2-HOPO, Neunpa, Octapa, PyPa, Porphyrin, and Deferoxamine; or a radionuclide complex thereof.

[0123] In some embodiments, R1is a chelating moiety selected from the list consisting of:1.4.7.10-tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTA); 1,4,7,10- tetraazacyclododecane-l,4,7-triacetic acid (DO3A); l,4,7,10-tetraazacyclododecane-l,7-diacetic acid (DO2 A); a,a',a",a"'-tetramethyl- 1 ,4,7, 10-tetraazacy clododecane- 1 ,4,7, 10-tetraacetic acid (DOTMA); 1 ,4,7, 10-tetrakis(carbamoylmethyl)- 1 ,4,7, 10-tetraazacy clododecane (DOTAM);1.4.7.10-tetraazacy clododecane- 1,4, 7, 10-tetrapropionic acid (DOTPA); 2,2',2"-(10-(2-amino-2- oxoethyl)- 1,4, 7, 10-tetraazacy clododecane- 1,4, 7-triyl)triacetic acid; 10-((6-carboxypyridin-2- yl)methyl)-l,4,7,10-tetra-azacyclododecane-l,4,7-triacetic acid (DO3Apic); 6,6'-(((pyridine-2,6- diylbis(methylene))bis((carboxymethyl)azanediyl))-bis(methylene))dipicolinic acid (EUpypa); 6,6',6",6"'-(((pyridine-2,6-diylbis(methylene))bis(azanetriyl))tetrakis(methylene))-tetrapicolinic acid (H4py4pa); and 3,6,9,12-tetrakis(carboxymethyl)-3,6,9,12-tetraazatetradecanedioic acid (TTHA); or a radionuclide complex thereof.

[0124] In some embodiments, R1comprises a chelating moiety with one of the following structures:calix[4]arene-tetracarboxylic acid),

[0125] In some embodiments, R1comprises a chelating moiety with one of the following structures:radionuclide complex thereof.

[0126] In some embodiments, R1comprises DOTA and is attached to the tumor targeting

[0127] In some embodiments, R1comprises DOTA and is attached to the tumor targeting moiety at the position shown:

[0128] In some embodiments, R1comprises DOTA and is attached to the tumor targeting moiety at the position shown:

[0129] It is understood that the immunoconjugates described herein comprise one or more R1moieties. In some embodiments, the immunoconjugates described herein comprise one or more than one R1moieties, wherein each R1moiety is the same.Linkers

[0130] In one embodiment, a chelating agent of the immunoconjugate is covalently linked to an antigen binding region, the heavy chain constant region, the immunoglobulin Fc region, or any combination thereof. In one embodiment, a chelating agent is covalently linked to the antigen binding region, the heavy chain constant region, the immunoglobulin Fc region, or any combination thereof directly. In one embodiment the chelating agent is covalently linked to the antigen binding arm through a linker that is covalently linked to the chelating agent and covalently linked to the antigen binding arm. In one embodiment, the linker is hydrophilic (e.g., a PEG chain). In one embodiment, the linker is hydrophobic (e.g., an alkyl or alkene chain). Chelators may belinked or coupled to the immunoconjugates as described in Sadiki, A. et al. “Site-specific conjugation of native antibody.” Antibody Therapeutics 2020, 3, 271-284.

[0131] In some embodiments, the immunoconjugate is formed through the attachment of the chelator-linker in a site-specific manner, directed into a specific amino acid or glycan residue. In some embodiments, the site-specific conjugation involves directed functionalization of a specific lysine residue in the framework region with the chelator-linker. In other embodiments, this residue may be functionalized with a different reactive functional group which then reacts in a second step with chelator-linker to furnish the immunoconjugate.

[0132] In some embodiments, a non-native cysteine residue is engineered into the framework of the antibody as a site for thiol directed conjugation to furnish the immunoconjugate. In some embodiments, other non-native amino acids or an amino acid sequence is engineered into the framework to serve as the attachment site for the chelator-linker or for a secondary reactive group upon which the chelator-linker will be conjugated to furnish the immunoconjugate.

[0133] In some embodiments, the chelator-linker is attached to a glutamine residue through the action of a transglutaminase enzyme. In other embodiments, a secondary reactive group is attached by transglutaminase upon which the chelator-linker is added to furnish the immunoconj ugate .

[0134] In some embodiments, the chelator-linker is attached by modifying one or more N- glycans with a reactive functional group through the action of a glycosidase, then conjugation of the chelator-linker to that site. In some embodiments, the glycan is modified through the action of P-galactosidase.

[0135] In one embodiment, the immunoconjugate comprises more than one chelating agent, which are the same or different.

[0136] In one embodiment, an immunoconjugate having more than one chelating agent has more than one chelating agent attached to the same antigen binding arm.

[0137] In one embodiment, an immunoconjugate having more than one chelating agent and less than eleven chelating agents has more than two chelating agents, more than three chelating agents, more than four chelating agents, more than five chelating agents, more than six chelating agents, more than seven chelating agents, more than eight chelating agents, or more than nine chelating agents. In one embodiment, the chelating agents are the same. In one embodiment, each antigen binding arm is linked directly or indirectly to more than one chelating agent.

[0138] In one embodiment, the chelating agent comprises a radioisotope chelating component and a functional group that allows for covalent attachment to the antigen binding arm. In oneembodiment the chelating agent further comprises a linker between the functional group and the radioisotope chelating component.

[0139] Linker considerations include the effect on physical or pharmacokinetic properties of the resulting radioimmunoconjugate, such as solubility, lipophilicity, hydrophilicity, hydrophobicity, stability (more or less stable as well as planned degradation), rigidity, flexibility, immunogenicity, modulation of antibody binding, and the like.

[0140] An optimal linker should link the carrier antibody to the radiochelator without impairing the functionality of either, provide a stable linkage in circulation, and degrade under specific conditions. Stability in circulation has proven to constitute a particularly important factor for success using this strategy.

[0141] In malignancies, the accessibility of tumor cells enables targeting by specific antibodies. Antigens that are expressed on the tumor cells but not on normal healthy cells allow selective targeting of the tumor cells while sparing the non-tumor cells. mAb tagged with shortlived radionuclides emitting short-ranged, high linear energy transfer particles represent an attractive means of treating tumors. These short-ranged particles may be capable of single-cell kill while sparing bystanders. High energy alpha-emitters used in radioimmunotherapy include211At (astatine-211),212Bi,213Bi, and225Ac . This latter isotope is an in vivo isotope generator, in that it has a long (10 day) half-life and decays via alpha-emission through three short-lived atoms, each of which yields an alpha particle.

[0142] In some instances, a limitation to the general use of tumor targeted225Ac radioimmunotherapy is the excessive radiation of normal tissues by the radioimmunoconjugate. With the exception of antibodies targeting leukemias, most (>99%) of the injected antibody dose remains circulating in the blood or is not associated with the target tumor. The prolonged circulation time of untargeted antibodies, typically days to weeks, results in radiation exposure of normal organs that catabolize or retain proteins and peptides, primarily liver and kidneys.

[0143] In the case of in vivo decay of alpha-emitters, an additional major potential obstacle to their safe use is the sequential release of three unchelated alpha-emitting daughter atoms following initial decay of the initial radionuclide. Uptake into organs such as the kidney or the liver could induce toxicity. An optimal linker should link the carrier mAb to the radiochelator without impairing the functionality of either, provide a stable linkage in circulation, and degrade under specific conditions. Stability in circulation has proven to constitute a particularly important factor for success using this strategy.Exemplary Conjugates

[0144] In an aspect, described herein is a compound of Formula (I), or a pharmaceutically acceptable salt thereof:Formula (I) wherein: at least one tertiary amide is present in the linear chain connecting R1to R8;R1is a radionuclide chelating moiety or a radionuclide complex thereof;X1is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-; m is 1, 2, 3, 4, 5, or 6; x is 0 or 1;R2is hydrogen, halogen, substituted or unsubstituted Ci-Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), -NRa-(substituted or unsubstituted Ci-Cealkyl), or substituted or unsubstituted Ci-Cioheteroalkyl, -©-(substituted or unsubstituted Ci- Cioheteroalkyl), -NRa-(substituted or unsubstituted Ci- Cioheteroalkyl), or C4- ©2opolyethylene glycol;X2is absent, -O-, -S-, -S(=O)2-, -NRa-, or -C(=O)N(Ra)-;L1is absent, Ci-Cioalkylene, ©2-Cioalkenylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, - S-, -S(=O)-, -S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, - NRbC(=O)-, -C(=O)NRb-, -OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, - NRbC(=N-Rb)NRb-, or -NRbC(=O)O-;L2is absent,Y1is absent,n is 0 or 1; each R3and R6is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-©2opolyethylene glycol, -C(=O)NH2, -C(=O)OH, or the side chain of an amino acid selected from thegroup consisting of: aspartic acid, glutamic acid, carboxyglutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with - C(=O)(unsubstituted or substituted Ci-C2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl-3-bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or -C(=0)NH-(C4-C2opolyethylene glycol); y is 0 or 1;t is 0 or 1; each Ra, Rb, Rc, Rd, R2, R4, R4a, R5, R7and R7ais independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci- Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, -La-(Re), or R3; each u is independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; each Lais independently absent or Ci-Cealkylene;Reis substituted or unsubstituted Cs-Cncycloalkyl, substituted or unsubstituted C2- Cnheteroycloalkyl, substituted or unsubstituted phenyl, or substituted or unsubstituted pyridinyl; or R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl; or R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl;X4is absent, -CH2-, -O-, -S-, or -NRC-; q is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;R8is a moiety capable of covalently conjugating with a biologic targeting moiety (RB);

[0145] In an aspect, described herein is a compound of Formula (I), or a pharmaceutically acceptable salt thereof:Formula (I) wherein: at least one tertiary amide is present in the linear chain connecting R1to R8;R1is a radionuclide chelating moiety or a radionuclide complex thereof;X1is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-; m is 1, 2, 3, 4, 5, or 6; x is 0 or 1;R2is hydrogen, halogen, substituted or unsubstituted Ci-Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), -NRa-(substituted or unsubstituted Ci-Cealkyl), or substituted or unsubstituted Ci-Cioheteroalkyl, -©-(substituted or unsubstituted Ci- Cioheteroalkyl), -NRa-(substituted or unsubstituted Ci- Cioheteroalkyl), or C4-C2opolyethylene glycol;X2is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)2-;L1is absent, Ci-Cioalkylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, -S-, -S(=O)-, -S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, -NRbC(=O)-, -C(=O)NRb-, - OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, -NRbC(=N-Rb)NRb-, or - NRbC(=O)O-;L2is absent,Y1is absent,n is 0 or 1; each R3and R6is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -C(=0)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carboxyglutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with - C(=O)(unsubstituted or substituted Ci-C2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl-3-bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or -C(=0)NH-(C4-C2opolyethylene glycol); y is 0 or 1;p is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;t is 0 or 1; each Ra, Rb, Rc, Rd, R2, R4, R4a, R5, R7and R7ais independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci- Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, -La-(Re), or R3; each u is independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; each Lais independently absent or Ci-Cealkylene;Reis substituted or unsubstituted Cs-Cncycloalkyl, substituted or unsubstituted C2- Cnheteroycloalkyl or substituted or unsubstituted phenyl; or R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl; or R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl;X4is absent, -CH2-, -O-, -S-, or -NRC-; q is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; andR8is a moiety capable of covalently conjugating with a biologic targeting moiety (RB).

[0146] In some embodiments,

[0147] In some embodiments, RBis an antibody or fragment thereof, peptide, nucleic acid, or small molecule.

[0148] In some embodiments, RBis an antibody or fragment thereof.

[0149] In some embodiments, R8is a moiety capable of conjugating with an amine (-NH2) or thiol (-SH) of an antibody or fragment thereof.

[0150] In some embodiments, R8is a moiety capable of conjugating with an amine (-NH2) of an antibody or fragment thereof and is selected from:,

[0153] In some embodiments, the compound of Formula (I) has the structure of Formula (la), or a pharmaceutically acceptable salt thereof:Formula (la).

[0154] In some embodiments, the compound of Formula (I) has the structure of Formula (lb), or a pharmaceutically acceptable salt thereof:Formula (lb).

[0155] In some embodiments, the compound of Formula (I) has the structure of Formula (Ic), or a pharmaceutically acceptable salt thereof:Formula (Ic).

[0156] In some embodiments, R8is a moiety capable of conjugating with a thiol (-SH) of an antibody or fragment thereof and is selected from:

[0157] In some embodiments,X5is absent, -C(=O)-, -O-, -NRa-, or -C(=O)N(Ra)-;L3is absent, Ci-Cioalkylene,u is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;X6is absent, -NR9- or -(CH2)g-NR9-; and R11is Cl, Br or I; and g is 1, 2, 3, or 4.

[0158] In some embodiments, the compound of Formula (I) has the structure of Formula (Id), a pharmaceutically acceptable salt thereof:Formula (Id).

[0159] In some embodiments, the compound of Formula (I) has the structure of Formula (le), a pharmaceutically acceptable salt thereof:Formula (le).

[0160] In some embodiments, the compound of Formula (I) has the structure of Formula (If), a pharmaceutically acceptable salt thereof:Formula (If).

[0161] In some embodiments, the compound of Formula (I) has the structure of Formula (Ig), a pharmaceutically acceptable salt thereof:Formula (Ig).

[0162] In some embodiments, X1is absent. In some embodiments, X1is -O-, -S-, -NRa-, or - C(=O)N(Ra)-. In some embodiments, X1is -O-, -S-, or -NRa-. In some embodiments, X1is -O-. In some embodiments, X1is -S-. In some embodiments, X1is -NRa-, wherein Rais hydrogen or Ci- Cealkyl. In some embodiments, X1is -NRa-, wherein Rais hydrogen. In some embodiments, X1is -NRa-, wherein Rais Ci-Cealkyl. In some embodiments, X1is -C(=O)N(Ra)-, wherein Rais hydrogen or Ci-Cealkyl. In some embodiments, X1is -C(=O)N(Ra)-, wherein Rais hydrogen. In some embodiments, X1is -C(=O)N(Ra)-, wherein Rais Ci-Cealkyl.

[0163] In some embodiments, X5is absent, -NR9-, -C(=O)-, -NR9C(=O), -C(=O)NR9-, In some embodiments, X5is absent or -NR9-. In some embodiments, X5is -NR9-, wherein R9is hydrogen. In some embodiments, X5is -NR9-, wherein R9is Ci-C4alkyl. In some embodiments, X5is absent.

[0164] In some embodiments, X6is -NR9-, wherein R9is hydrogen. In some embodiments, X6is -NR9-, wherein R9is Ci-C4alkyl. In some embodiments, X6is -(CH2)g-NR9-; and g is 1, 2, 3, or 4.

[0165] In some embodiments, x is 0. In some embodiments, x is 1.

[0166] In some embodiments, the compound of Formula (I) has the structure of Formula (II), or a pharmaceutically acceptable salt thereof:Formula (II).

[0167] In some embodiments, the compound of Formula (II) has the structure of Formula (III), or a pharmaceutically acceptable salt thereof:Formula (III) wherein:X2is absent, -CH2-, or -O-; and X3is absent, -CH2-, or -O-.

[0168] In some embodiments, the compound of Formula (II) has the structure of Formula (IV), or a pharmaceutically acceptable salt thereof:Formula (IV) wherein:X2is absent, -CH2-, or -O-; X3is absent, -CH2-, or -O-; and

[0169] In some embodiments, the compound of Formula (I) has the structure of Formula (V), or a pharmaceutically acceptable salt thereof:Formula (V).

[0170] In some embodiments, the compound of Formula (V) has the structure of Formula (VI), or a pharmaceutically acceptable salt thereof:Formula (VI) wherein,X2is absent, -CH2-, or -O-; and X3is absent, -CH2-, or -O-.

[0171] In some embodiments, the compound of Formula (I) has the structure of Formula (VII), or a pharmaceutically acceptable salt thereof:Formula (VII) wherein,X2is absent, -CH2-, or -O-; and X3is absent, -CH2-, or -O-;

[0172] In some embodiments, the compound of Formula (II) has the structure of Formula (V), or a pharmaceutically acceptable salt thereof:complex thereof.

[0173] In some embodiments, the compound of Formula (II) has the structure of Formula (V), or a pharmaceutically acceptable salt thereof:nuclide complex thereof.In some embodiments,. In some embodiments,. , . In some embodiments,. In some embodiments,is

[0174] In some embodiments, p is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11. In some embodiments, p is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. In some embodiments, p is 1, 2, 3, 4, 5, 6, 7, 8, or 9. In some embodiments, p is 1, 2, 3, 4, 5, 6, 7, or 8. In some embodiments, p is 1, 2, 3, 4, 5, 6, or 7. In some embodiments, p is 1, 2, 3, 4, 5, or 6. In some embodiments, p is 1, 2, 3, 4, or 5. In some embodiments, p is 1, 2, 3, or 4. In some embodiments, p is 1, 2, or 3. In some embodiments, p is 1, or 2. In some embodiments, p is 5 or 10. In some embodiments, p is 1. In some embodiments, p is 2. In some embodiments, p is 3. In some embodiments, p is 4. In some embodiments, p is 5. In some embodiments, p is 6. In some embodiments, p is 7. In some embodiments, p is 8. In some embodiments, p is 9. In some embodiments, p is 10. In some embodiments, p is 11. In some embodiments, p is 12.

[0175] In some embodiments,, In some embodiments, Y1is, In some embodiments, Y1is[, ,

[0178] In some embodiments, L1is Ci-Cioalkylene and X1is absent or -O-. In some embodiments, L1is Ci-Cioalkylene and X1is absent. In some embodiments, L1is Ci-Cioalkylene and X1is -O-. In some embodiments, L1is C2-Cioalkenylene and X1is absent or -O-.

[0179] In some embodiments, X2is -O-, -S-, -NRa-, or -C(=O)N(Ra)-. In some embodiments, X2is -O-, -S-, or -NRa-. In some embodiments, X2is -O-. In some embodiments, X2is -S-. In some embodiments, X2is -NRa-, wherein Rais hydrogen or substituted or unsubstituted Ci- Cealkyl. In some embodiments, X2is -NRa-, wherein Rais hydrogen. In some embodiments, X2is -NRa-, wherein Rais substituted or unsubstituted Ci-Cealkyl. In some embodiments, X2is - C(=O)N(Ra)-, wherein Rais hydrogen or substituted or unsubstituted Ci-Cealkyl. In some embodiments, X2is -C(=O)N(Ra)-, wherein Rais hydrogen. In some embodiments, X2is - C(=O)N(Ra)-, wherein Rais substituted or unsubstituted Ci-Cealkyl. In some embodiments, X2is absent.

[0180] In some embodiments, L1is Ci-Cioalkylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, -S-, -S(=O)-, - S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, -NRbC(=O)-, -C(=O)NRb-, - OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, -NRbC(=N-Rb)NRb-, or -NRbC(=O)O-. Insome embodiments, L1is Ci-Cioalkylene. In some embodiments, L1is -CH2-, -CH2CH2-, - CH2CH2CH2-, or -CH2(CH2)2CH2-. In some embodiments, L1is -CH2-. In some embodiments, L1is -CH2CH2-. In some embodiments, L1is -CH2CH2CH2-. In some embodiments, L1is - CH2(CH2)2CH2-. In some embodiments, L1is Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, -S-, -S(=O)-, -S(=O)2-, - S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, -NRbC(=O)-, -C(=O)NRb-, -OC(=O)NRb-, - NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, -NRbC(=N-Rb)NRb-, or -NRbC(=O)O-. In some embodiments, L1is Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, -S-, -S(=O)-, -S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)- , -NRb-, or -P(=O)ORb-.

[0181] In some embodiments, L1is absent.R3

[0182] In some embodiments, L2isIn some embodiments, L2isRC. In someO R3embodiments, L2isRC. In some embodiments, L2is absent., embodiments,

[0184] In some embodiments, R3is independently hydrogen, -C(=O)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carb oxy glutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with -C(=O)(unsubstituted or substituted Ci-C2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl- 3 -bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or -C(=0)NH-(C4- C2opolyethylene glycol). In some embodiments, R3is independently the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carb oxy glutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine. In some embodiments, R3is independently hydrogen, -C(=0)NH2, or -C(=O)OH. In some embodiments, R3is independently -C(=0)NH2. In some embodiments, R3is independently -C(=O)OH. In some embodiments, R3is independently hydrogen.[,

[0189] In some embodiments,

[0190] In some embodiments,

[0191] In some embodiments, L3is -(CH2)P- or. In some embodiments,some embodiments, L3is -(CH2)P-.

[0192] In some embodiments, L3is absent.

[0193] In some embodiments, p is 1. In some embodiments, p is 2. In some embodiments, p is 3. In some embodiments, p is 4. In some embodiments, p is 5. In some embodiments, p is 6. In some embodiments, p is 7. In some embodiments, p is 8. In some embodiments, p is 9. In some embodiments, p is 10. In some embodiments, p is 11. In some embodiments, p is 12.

[0194] In some embodiments, X3is -CH2-, -O-, -S-, or -NRd-. In some embodiments, X3is - CH2-. In some embodiments, X3is -O-. In some embodiments, X3is -S-. In some embodiments, X3is -NRd-, wherein Rdis hydrogen or substituted or unsubstituted Ci-Cealkyl. In some embodiments, X3is -NRd-, wherein Rdis hydrogen. In some embodiments, X3is -NRd-, wherein Rdis substituted or unsubstituted Ci-Cealkyl. In some embodiments, X3is absent.

[0195] In some embodiments, L3is -CH2-. In some embodiments, L3is -(CH )2-. In some embodiments, L3is -(CH2)3-. In some embodiments, L3is -(CH2)4-. In some embodiments, L3is - (CH2)5-. In some embodiments, L3is -(CH2)e-. In some embodiments, L3is -(CJb)?-. In some embodiments, L3is -(CH2)s-. In some embodiments, L3is -(CH2)9-. In some embodiments, L3is - (CH2)IO-. In some embodiments, L3is -(CH2)n-. In some embodiments, L3is -(CH2)i2-.

[0196] In some embodiments, L3is. In some embodiments, p is 1. In some embodiments, p is 2. In some embodiments, p is 3. In some embodiments, p is 4. In some embodiments, p is 5. In some embodiments, p is 6. In some embodiments, p is 7. In some embodiments, p is 8. In some embodiments, p is 9. In some embodiments, p is 10. In some embodiments, p is 11. In some embodiments, p is 12.

[0198] In some embodiments,. In some embodiments,,, embodiments,. In some embodiments,

[0200] In some embodiments, q is 0. In some embodiments, q is 1. In some embodiments, q is 2. In some embodiments, q is 3. In some embodiments, q is 4. In some embodiments, q is 5. In some embodiments, q is 6. In some embodiments, q is 7. In some embodiments, q is 8. In some embodiments, q is 9. In some embodiments, q is 10. In some embodiments, q is 11. In some embodiments, q is 12.

[0201] In some embodiments, X4is -CH2-, -O-, -S-, or -NRd-. In some embodiments, X4is - CH2-. In some embodiments, X4is -O-. In some embodiments, X4is -S-. In some embodiments, X4is -NRd-, wherein Rdis hydrogen or substituted or unsubstituted Ci-Cealkyl. In someembodiments, X4is -NRd-, wherein Rdis hydrogen. In some embodiments, X4is -NRd-, wherein Rdis substituted or unsubstituted Ci-Cealkyl. In some embodiments, X4is absent

[0202] In some embodiments,. In some embodiments,,, embodiments,. In some embodiments,, embodiments,. In some embodiments,, embodiments,. In some embodiments,

[0204] In some embodiments, X3is -CH2- and X4is -O-; or X3is -O- and X4is -CH2-. In some embodiments, X3is -CH2- and X4is -O-. In some embodiments, X3is -O- and X4is -CH2-.

[0205] In some embodiments,In some embodiments,In some embodiments,

[0206] In some embodiments, R4and R4aare independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4- C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re). In some embodiments, R4and R4aare independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, R4and R4aare independently -CH3, -CH2CH3, -C(CH3)2, -CH2CH(CH3)2, or -CH2C(CH3)3. In some embodiments, R4and R4aare independently -CH3. In some embodiments, and R4and R4aare independently -CH2CH3. In some embodiments, R4and R4aare independently -C(CH3)2. In some embodiments, R4and R4aare independently substituted, or substituted or unsubstituted Ci- Ceheteroalkyl. In some embodiments, R4and R4aare independently C4-C2opolyethylene glycol. In some embodiments, R4and R4aare independently -(CH2CH2O)U-CH3. In some embodiments, R4and R4aare independently-La-(Re).

[0207] In some embodiments, R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl. In some embodiments, R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-C6heteroycloalkyl. In some embodiments, R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted Cvheteroycloalkyl. In some embodiments, R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted Ceheteroycloalkyl. In some embodiments, R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted Csheteroycloalkyl.

[0208] In some embodiments, R7and R7aare independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4- C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re). In some embodiments, R7and R7aare independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, R7and R7aare independently -CH3, -CH2CH3, -C(CH3)3, -CH2CH(CH3)2, or -CH2C(CH3)3. In some embodiments, R7and R7aare independently -CH3. In some embodiments, R7and R7aareindependently -CH2CH3. In some embodiments, R7and R7aare independently -C(CH3)2. In some embodiments, R7and R7aare independently substituted, or substituted or unsubstituted Ci- Ceheteroalkyl. In some embodiments, R7and R7aare independently C4-C2opolyethylene glycol. In some embodiments, R7and R7aare independently -(CH2CH2O)U-CH3. In some embodiments, R7and R7aare independently-La-(Re).

[0209] In some embodiments, R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl. In some embodiments, R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-C6heteroycloalkyl. In some embodiments, R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted Cvheteroycloalkyl. In some embodiments, R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted Ceheteroycloalkyl. In some embodiments, R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted Csheteroycloalkyl.

[0210] In some embodiments, each R4and R7is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4- C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re). In some embodiments, each R4and R7is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, or -La-(Re). In some embodiments, each R4and R7is independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, each R4and R7is independently -CH3, -CH2CH3, -C(CH3)2, -CH2CH(CH3)2, or - CH2C(CH3)3. In some embodiments, each R4and R7is independently -CH3. In some embodiments, each R4and R7is independently -CH2CH3. In some embodiments, each R4and R7is independently -C(CH3)2. In some embodiments, each R4and R7is independently -La-(Re).

[0211] In some embodiments, R4is hydrogen; and R7is substituted or unsubstituted Ci- Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re). In some embodiments, R4is hydrogen; and R7is substituted or unsubstituted Ci-Cealkyl, or -La-(Re). In some embodiments, R4is hydrogen; and R7is substituted or unsubstituted Ci-Cealkyl. In some embodiments, R4is hydrogen; and R7is -La-(Re).

[0212] In some embodiments, R4is substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re); and R7is hydrogen. In some embodiments, R4is substituted or unsubstituted Ci-Cealkyl, or -La-(Re); and R7is hydrogen. In some embodiments, R4is substituted or unsubstituted Ci- Cealkyl; and R7is hydrogen. In some embodiments, R4is -La-(Re); and R7is hydrogen.

[0213] In some embodiments, each Reis independently selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, bicyclo[l.l. l]pentanyl, bicyclo[2.1.1]hexanyl, bicyclo[3.1.1]heptanyl, bicyclo[2.2.2]octanyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, 2- oxabicyclo[l.l. l]pentanyl, 2-oxabicyclo[2.1.1]hexanyl, 2-oxabicyclo[2.2.2]octanyl, azetidinyl, pyrrolidinyl, piperidinyl, phenyl, pyridinyl, and adamantyl, wherein each Reis independently optionally substituted.

[0214] In some embodiments, each Reis independently selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, bicyclo[l.l. l]pentanyl, bicyclo[2.1.1]hexanyl, bicyclo[3.1.1]heptanyl, bicyclo[2.2.2]octanyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, 2- oxabicyclo[l.l. l]pentanyl, 2-oxabicyclo[2.1.1]hexanyl, 2-oxabicyclo[2.2.2]octanyl, azetidinyl, pyrrolidinyl, piperidinyl, phenyl, and adamantyl, wherein each Reis independently optionally substituted.

[0215] In some embodiments, each Reis independently selected from, and, wherein each Reis independently optionally substituted. In some embodiments, eachReis independently selected from, wherein eachReis independently optionally substituted.

[0216] In some embodiments, each Reis independently selected from, wherein each Reis independently optionally substituted. In some embodiments, each Reisindependently selected fromindependently optionally substituted.

[0217] In some embodiments, each Lais independently absent, -CH2-, -CH2CH2-, -CH2CH2CH2-, -CH2CH2CH2CH2-,-CH2CH2CH2CH2CH2-, or -CH2CH2CH2CH2CH2CH2-. In some embodiments, each Lais independently -CH2-, -CH2CH2-, -CH2CH2CH2-, - CH2CH2CH2CH2-,-CH2CH2CH2CH2CH2-, or -CH2CH2CH2CH2CH2CH2-. In some embodiments, each Lais independently absent, -CH2-, -CH2CH2-, or -CH2CH2CH2-. In some embodiments, eachLais independently absent.(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)II-CH3, or -(CH2CH2O)I2-CH3.

[0220] In some embodiments, each R4and R7is independentlysome embodiments, each R4and R7isIn some embodiments, each R4and R7is-(CH2CH2O)2-CH3, -(CH2CH2O)3-CH3, -(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)II-CH3, or -(CH2CH2O)I2-CH3. In some embodiments, R4is hydrogenembodiments, R4is hydrogen,insome embodiments, R4is hydrogen I or

[0224] In some embodiments, R7is hydrogen-(CH2CH2O)3-CH3, -(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)II-CH3, or - (CH2CH2O)I2-CH3.

[0225] In some embodiments, R7is hydrogen-(CH2CH2O)2-CH3, -(CH2CH2O)3-CH3, -(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -, , , , -(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)II-CH3, or -(CH2CH2O)I2-CH3. In some embodiments, each R7is independently hydrogendiments, R7is hydrogen,In some embodiments, R7is

[0227] In some embodiments, each R6is independently hydrogen, -C(=O)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carb oxy glutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with -C(=O)(unsubstituted or substituted Ci-C2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with - C(=O)NH-(2,4,6-trimethyl-3-bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci- C2oalkylene), or -C(=0)NH-(C4-C2opolyethylene glycol). In some embodiments, R6is independently the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carboxyglutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine. In some embodiments, R6is independently hydrogen, -C(=O)NH2, or -C(=O)OH. In some embodiments, R6is independently -C(=O)NH2. In some embodiments, R5is independently -C(=O)OH. In some embodiments, R6is independently hydrogen.

[0230] In some embodiments, R2is hydrogen, halogen, substituted or unsubstituted Ci- Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), or C4-C2opolyethylene glycol. In some embodiments, R2is halogen. In some embodiments, R2is -Cl, -F, or -Br. In some embodiments, R2is substituted or unsubstituted Ci-Cealkyl. In some embodiments, R2is -CH3, -CH2CH3, or - C(CH3)2. In some embodiments, R2is -CH3. In some embodiments, R2is -CH2CH3. In some embodiments, R2is -C(CH3)2. In some embodiments, R2is -©-(substituted or unsubstituted Ci- Cealkyl). In some embodiments, R2is -OCH3. In some embodiments, R2is C4-C2opolyethylene glycol. In some embodiments, R2is C4-Ci8polyethylene glycol. In some embodiments, R2is C4- Ciepoly ethylene glycol. In some embodiments, R2is C4-Ci2polyethylene glycol. In some embodiments, R2is C4-Ciopolyethylene glycol. In some embodiments, R2is C4-Cepolyethylene glycol. In some embodiments, R2is hydrogen.

[0231] In some embodiments, each R5is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl. In some embodiments, each R5is independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, each R5is independently -CH3, -CH2CH3, or -C(CH3)2. In some embodiments, each R5is independently substituted Ci-Ceheteroalkyl. In some embodiments, each R5is independently C4-C2opolyethylene glycol. In some embodiments, each R5is independently -(CH2CH2©)U-CH3. In some embodiments, each R5is independently -La-(Re). In some embodiments, each R5is independently R3. In some embodiments, each R5is independently hydrogen

[0232] In some embodiments, m is 1, 2, 3, or 4. In some embodiments, m is 1. In some embodiments, m is 2. In some embodiments, m is 3. In some embodiments, m is 4.

[0233] In some embodiments, each Rais independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl. In some embodiments,each Rais independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, each Rais independently -CH3, -CH2CH3, or -C(CH3)2. In some embodiments, each Rais independently substituted Ci-Ceheteroalkyl. In some embodiments, each Rais independently C4-C2opolyethylene glycol. In some embodiments, each Rais independently -(CH2CH2O)U-CH3. In some embodiments, each Rais independently -La-(Re). In some embodiments, each Rais independently R3. In some embodiments, each Rais hydrogen.

[0234] In some embodiments, each Rbis independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl. In some embodiments, each Rbis independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, each Rbis independently -CH3, -CH2CH3, or -C(CH3)2. In some embodiments, each Rbis independently substituted Ci-Ceheteroalkyl. In some embodiments, each Rbis independently C4-C2opolyethylene glycol. In some embodiments, each Rbis independently -(CH2CH2O)U-CH3. In some embodiments, each Rbis independently -La-(Re). In some embodiments, each Rbis independently R3. In some embodiments, each Rbis hydrogen.

[0235] In some embodiments, each Rcis independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl. In some embodiments, each Rcis independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, each Rcis independently -CH3, -CH2CH3, or -C(CH3)2. In some embodiments, each Rais independently substituted Ci-Ceheteroalkyl. In some embodiments, each Rcis independently C4-C2opolyethylene glycol. In some embodiments, each Rcis independently -(CH2CH2O)U-CH3. In some embodiments, each Rcis independently -La-(Re). In some embodiments, each Rcis independently R3. In some embodiments, each Rcis independently hydrogen.

[0236] In some embodiments, each Rdis independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl. In some embodiments, each Rdis independently substituted or unsubstituted Ci-Cealkyl. In some embodiments, each Rdis independently -CH3, -CH2CH3, or -C(CH3)2. In some embodiments, each Rdis independently substituted Ci-Ceheteroalkyl. In some embodiments, each Rdis independently C4-C2opolyethylene glycol. In some embodiments, each Rdis independently -(CH2CH2O)U-CH3. In some embodiments, each Rdis independently -La-(Re). In some embodiments, each Rdis independently R3. In some embodiments, each Rdis independently hydrogen.

[0237] Exemplary compounds for use in preparing immunoconjugates described herein include the compounds depicted in Table A.Table A

[0238] Exemplary compounds for use in preparing immunoconjugates described herein include the compounds depicted in Table B.Table B

[0239] In Tables0

[0240] In some embodiments,in Tables A and / or B is replaced with0 some embodiments,in Tables A and / or B is replaced with0 some embodiments,in Tables A and / or B is replaced withO me embodiments,in Tables A and / or B is replaced with. some embodiments,in Tables A and / or B is replaced with. , in Tables A and / or B is replaced with. , in TablesA and / or B is replaced with. In some embodiments,some0. ,F is replaced with, in0Tables A and B is replaced withi. In some embodiments,in Tables A0 and / or B is replaced with. In some embodiments,in Tables A and / or B is0

[0241] In some embodiments,in Tables A and / or B is replacedsome embodiments,in Tables A and / or B is replaced with. , p0fl\A_ / TFPIn some embodiments,uin Tables A and / or B is replaced with. In some0 embodiments,in Tables A and / or B is replaced with

[0242] Additional compounds described herein are depicted in Table C.Table C.Radionuclides

[0243] In some embodiments, the radionuclide in the immunoconjugates described herein is an Auger electron-emitting radionuclide. In some embodiments, the radionuclide is an a-emitting radionuclide. In some embodiments, the radionuclide is a P-emitting radionuclide. In some embodiments, the radionuclide is a y-emitting radionuclide. In some embodiments, the type of radionuclide used in a non-peptide targeted therapeutic compound can be tailored to the specific type of cancer, the type of targeting moiety (e.g., non-peptide ligand), etc. Radionuclides that undergo a-decay emit a-particles (helium ions with a +2 charge) from their nuclei. As a result of a-decay the daughter nuclide has 2 protons less and 2 neutrons less than the parent nuclide. This means that in a-decay, the proton number is reduced by 2 while the nucleon number is reduced by 4. Radionuclides that undergo P-decay emit P-particles (electrons) from their nuclei. During P- decay, one of the neutrons changes into a proton and an electron. The proton remains in the nucleus while the electron is emitted as a P-particle. This means that in P-decay, the nucleus loses a neutron but gains a proton. In y-decay, a nucleus in an excited state (higher energy state) emits a y-ray photon to change to a lower energy state. There is no change in the proton number and nucleon number during the y-decay. The emission of y-rays often accompanies the emission of a-particles and P-particles.

[0244] Auger electrons (AEs) are very low energy electrons that are emitted by radionuclides that decay by electron capture (EC) (e.g.,l uIn , gallium-67 (67Ga) , technetium-99m (99mTc) , platinum- 195m (195mPt) , iodine-125 (125I), and iodine-123 (123I). This energy is deposited over nanometer-micrometer distances, resulting in high linear energy transfer that is potent for causinglethal damage in cancer cells. Thus, AE-emitting radiotherapeutic agents have great potential for treatment of cancer.

[0245] P-Particles are electrons emitted from the nucleus. They typically have a longer range in tissue (of the order of 1-5 mm) and are the most frequently used.

[0246] a-Particles are helium nuclei (two protons and two neutrons) that are emitted from the nucleus of a radioactive atom. Depending on their emission energy, they can travel 50-100 pm in tissue. They are positively charged and are orders of magnitude larger than electrons. The amount of energy deposited per path length travelled (designated ‘linear energy transfer’) of a-particles is approximately 400 times greater than that of electrons. This leads to substantially more damage along their path than that caused by electrons. An a-particle track leads to a preponderance of complex and largely irreparable DNA double-strand breaks. The absorbed dose required to achieve cytotoxicity relates to the number of a-particles traversing the cell nucleus. With use of this as a measure, cytotoxicity may be achieved with a range of 1 to 20 a-particle traversals of the cell nucleus. The resulting high potency, combined with the short range of a-particles (which reduces normal organ toxicity), has led to substantial interest in developing a-particle-emitting agents. The a-particle emitters typically used include212Bi ,212Pb,213Bi ,225Ac,223Ra, and229Th.

[0247] In some embodiments, the radionuclide is a diagnostic or therapeutic radionuclide.Representative Radionuclides

[0248] In some embodiments, the radionuclide is an Auger electron-emitting radionuclide. In some embodiments, the radionuclide is an Auger electron-emitting radionuclide that isi nIn,67Ga,68Ga, 9"mTc, or195mPt. In some embodiments, the radionuclide is an Auger electron-emitting radionuclide that isU 1ln,67Ga,68Ga, or "mTc.

[0249] In some embodiments, the radionuclide is an a-emitting radionuclide. In some embodiments, the radionuclide is an a-emitting radionuclide that is225Ac,213Bi,223Ra, or212Pb. In some embodiments, the radionuclide is an a-emitting radionuclide that is225Ac. In some embodiments, the radionuclide is an P-emitting radionuclide. In some embodiments, the radionuclide is a P-emitting radionuclide that is90Y,177Lu, rhenium-186 (186Re), rhenium-188 (188Re),64Cu,67Cu,153Sm ,89Sr , gold-198 (198Au), erbium-169 (169Er), dysprosium- 165 (165Dy), "mTc,89Zr, or manganese-52 (52Mn). In some embodiments, the radionuclide is a P-emitting radionuclide that is90Y,177Lu, "mTc, or89Zr.

[0250] In some embodiments, the radionuclide is a y-emitting radionuclide. In some embodiments, the radionuclide is a y-emitting radionuclide that is60Co cobalt-60 (60Co), palldium- 103 (103Pd), cesium-137 (137Cs), ytterbium-169 (169Yb), iridium-192 (192Ir),212B1,213Bi, or226Ra.Radioimmunoconj ugates

[0251] In one embodiment, the invention provides immunoconjugates. In one embodiment, the immunoconjugates are capable of delivering a-emitters in vivo when so labeled, linked or loaded with an a-emitter. In one embodiment, the immunoconjugates are also capable of delivering other radioisotopes (P-emitters, and / or y-emitters), and / or other atoms in vivo, when so labeled, linked or loaded. In one embodiment, the immunoconjugates are capable of delivering imaging metals (e.g.,U 1ln,89Zr,64Cu,68Ga, or134Ce) in vivo when so labeled, linked or loaded.

[0252] The immunoconjugates of the current disclosure may be loaded with a radioisotope for a therapeutic or diagnostic effect. In certain embodiments, the chelator may further comprise a radioisotope. In certain embodiments, the radioisotope is an alpha emitter. In certain embodiments, the radioisotope is an alpha emitter selected from the list consisting of225Ac,223Ra,224Ra,227Th,212Pb,212Bi, and213Bi. In certain embodiments, the radioisotope is225Ac. In certain embodiments, the radioisotope is an beta emitter. In certain embodiments, the radioisotope is a beta emitter selected from177Lu,90Y,67Cu, and153Sm.

[0253] Also described herein is a method of making a radioimmunoconjugate comprising loading or complexing an immunoconjugate of the current disclosure to a radioisotope. In certain embodiments, the radioisotope is an alpha emitter. In certain embodiments, the radioisotope is an alpha emitter selected from the list consisting of225Ac,223Ra,224Ra,227Th,212Pb,212Bi, and213Bi. In certain embodiments, the radioisotope is225Ac. In certain embodiments, the radioisotope is an beta emitter. In certain embodiments, the radioisotope is a beta emitter selected from177Lu,90Y,67Cu, and153Sm.

[0254] In one aspect, the invention provides a radioimmunoconjugate, comprising an immunoconjugate of the invention and an a-emitting radioisotope. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is selected from the group comprising:225Ac,223Ra,224Ra,227Th,212Pb,212Bi, and213Bi. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is selected from the group consisting of:225Ac,223Ra,224Ra,227Th,212Pb,212Bi, and213Bi. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is225Ac. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is223Ra. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is224Ra. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is227Th. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is212Pb. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is212Bi. In one embodiment, the a-emitting radioisotope of the radioimmunoconjugate is213Bi.

[0255] In some embodiments, the immunoconjugate of the present invention is combined with a radioisotope to provide a radioimmunoconjugate of the invention. In some embodiments, the radioisotope is225Ac,86Y,90Y,177Lu,186Re,188Re,89Sr,153Sm,213Bi,213Po,212Bi,223Ra,224Ra,227Th,149Tb,68Ga,64Cu,67Cu,89Zr,137Cs,212Pb, or103Pd. In some embodiments, the radioisotope is an alpha emitter, such as, e.g.,225Ac,223Ra,224Ra,227Th,212Pb,212Bi, and213Bi. In some embodiments, the radioisotope is a beta particle emitter, such as, e.g.,177Lu,90Y,67Cu,153Sm. In some embodiments, the radioisotope is both an alpha particle emitter and a beta and / or gamma particle emitter. In some embodiments, the radioisotope is both a beta particle emitter and a gamma particle and / or photon emitter. In some embodiments, the radioimmunoconjugate is labeled, linked or loaded with, and accordingly comprises, both an a-emitter and a P-emitter. In some embodiments, the radioisotope is selected for use in radio-imaging, such as, e.g., from among68Ga,64Cu,89Zr,n iIn, and134Ce.

[0256] The immunoconjugates and radioimmunoconjugates of the invention may comprise other cargos or payloads besides a radioisotope, including various cytotoxic agents, such as, e.g., a small molecule chemotherapeutic agent, cytotoxic antibiotic, alkylating agent, antimetabolite, topoisomerase inhibitor, and / or tubulin inhibitor. For example, an immunoconjugate of the invention may be used to deliver a non-radioisotope cytotoxin to a target cell. Non-limiting examples of cytotoxic agents include aziridines, cisplatins, tetrazines, procarbazine, hexamethylmelamine, vinca alkaloids, taxanes, camptothecins, etoposide, doxorubicin, mitoxantrone, teniposide, novobiocin, aclarubicin, anthracyclines, actinomycin, bleomycin, plicamycin, mitomycin, daunorubicin, epirubicin, idarubicin, dolastatins, maytansines, docetaxel, adriamycin, calicheamicin, auristatins, pyrrolobenzodiazepine, carboplatin, 5 -fluorouracil (5-FU), capecitabine, mitomycin C, paclitaxel, l,3-Bis(2-chloroethyl)-l-nitrosourea (BCNU), rifampicin, cisplatin, methotrexate, and gemcitabine.

[0257] In some embodiments, a radioimmunoconjugate of the invention comprises a radioisotope selected from the group comprising225Ac,86Y,90Y,177Lu,186Re,188Re,89Sr,153Sm,213Bi,213Po,211At,212Bi,223Ra,224Ra,227Th,149Tb,68Ga,64Cu,67Cu,89Zr,137Cs,212Pb, and103Pd.

[0258] In some embodiments, a radioimmunoconjugate of the invention comprises a radioisotope selected from the group consisting of225Ac,86Y,90Y,177Lu,186Re,188Re,89Sr,153Sm,213Bi,213Po,211At,212Bi,223Ra,224Ra,227Th,149Tb,68Ga,64Cu,67Cu,89Zr,137Cs,212Pb, and103Pd.

[0259] In some embodiments, the radioisotope is an alpha-particle-emitting radioisotope comprises225Ac,223Ra,224Ra,227Th,212Pb,212Bi, or213Bi.

[0260] In some embodiments, the radioisotope is an alpha-particle-emitting radioisotope selected from the group consisting of225Ac,223Ra,224Ra,227Th,212Pb,212Bi, and213Bi.

[0261] Further embodiments of the immunoconjugates, antigen binding regions, and heavy chain variable regions are described below.

[0262] In some embodiments, the immunoconjugate comprises a dimerization domain or motif. In some further embodiments, the dimerization domain or motif is in a variant constant region, linker or hinge region.

[0263] The skilled worker can engineer multimeric immunoconjugates of the present invention using approaches and methods known in the art. For example, engineered cysteine residues can form covalent bonds thereby stabilizing multimeric structures that spontaneously assemble (see e.g., Glockshuber R et al., Biochemistry 29: 1362-7 (1990)). For example, the introduction of cysteine residues at specific locations may be used to create disulfide stabilized structures like Cys-diabodies, scFv' multimers, VHH multimers, VNAR multimers, and IgNAR multimers such as, e.g., by adding the following amino acid residues: GGGGC (SEQ ID NO: 260) and SGGGGC (SEQ ID NO: 261) (Tai M et al., Biochemistry 29: 8024-30 (1990); Caron P et al., J Exp Med 176: 1191-5 (1992); Shopes B, J Immunol 148: 2918-22 (1992); Adams G et al., Cancer Res 53 : 4026-34 (1993); McCartney J et al., Protein Eng 18: 301-14 (1994); Perisic O et al., Structure 2: 1217-26 (1994); George A et al., Proc Natl Acad Sci USA 92: 8358-62 (1995); Tai M et al., Cancer Res (Suppl) 55: 5983-9 (1995); Olafsen T et al., Protein Eng Des Sei 17: 21- 7 (2004)).

[0264] Alternatively, two or more polypeptide chains may be linked together using polypeptide domains which self-associate or multimerize with each other (see e.g., US 6,329,507). For example, the addition of carboxy -terminal multimerization domains has been used to construct multivalent proteins comprising immunoglobulin domains, such as, e.g., scFvs, autonomous VH domains, VHHS, VNARS, and IgNARs. Examples of self-associating domains known to the skilled worker include immunoglobulin constant domains (such as knobs-into-holes, electrostaticsteering, and IgG / IgA strand exchange), immunoglobulin Fab chains (e.g., (Fab-scFv)2 and (Fab’ scFv)?), immunoglobulin Fc domains (e.g., (scDiabody-Fc)2, (scFv-Fc)2 and scFv-Fc-scFv), immunoglobulin CHX domains, immunoglobulin CHI -3 regions, immunoglobulin CH3 domains (e.g., (scDiabody-CH3)2, LD minibody, and Flex -minibody), immunoglobulin CH4 domains, CHCL domains, amphiphilic helix bundles (e.g., scFv-HLX), helix-turn-helix domains (e.g., scFv-dHlx), coiled-coil structures including leucine zippers and cartilage oligometric matrix proteins (e.g., scZIP), cAMP-dependent protein kinase (PKA) dimerization and docking domains (DDDs) combined with an A kinase anchor protein (AKAP) anchoring domain (AD) (also referred to as “dock-and-lock” or “DNL”), streptavidin, verotoxin B multimerization domains, tetramerization regions from p53, and bamase-barstar interaction domains (Pack P, Pliickthun A, Biochemistry 31 : 1579-84 (1992); Holliger P et al., Proc Natl Acad Sci USA 90: 6444-8 (1993); Kipriyanov S et al., Hum Antibodies Hybridomas 6: 93-101 (1995); de Kruif J, Logtenberg T, J Biol Chem 271 : 7630-4 (1996); Hu S et al., Cancer Res 56: 3055-61 (1996); Kipriyanov S et al., Protein Eng 9: 203-11 (1996); Rheinnecker M et al., J Immunol 157: 2989-97 (1996); Tershkikh A et al., Proc Natl Acad Sci USA 94: 1663-8 (1997); Muller K et al., FEBS Lett 422: 259-64 (1998); Cloutier S et al., Mol Immunol 37: 1067-77 (2000); Li S et al., Cancer Immunol Immunother 49: 243-52 (2000); Schmiedl A et al., Protein Eng 13: 725-34 (2000); Schoonjans R et al., J Immunol 165: 7050-7 (2000); Borsi L et al., Int J Cancer 102: 75-85 (2002); Deyev S et al., Nat Biotechnol 21 : 1486-92 (2003); Wong W, Scott J, Nat Rev Mol Cell Biol 5: 959-70 (2004); Zhang J et al., J Mol Biol 335: 49-56 (2004); Baillie G et al., FEBS Letters 579: 3264-70 (2005); Rossi E et al., Proc Natl Acad Sci USA 103: 6841-6 (2006); Simmons D et al., J Immunol Methods 315: 171-84 (2006); Braren I et al., Biotechnol Appl Biochem 47: 205-14 (2007); Chang C et al., Clin Cancer Res 13: 5586-91s (2007); Liu M et al., Biochem J 406: 237-46 (2007); Zhang J et al., Protein Expr Purif 65: 77-82 (2009); Bell A et al., Cancer Lett 289: 81-90 (2010); Iqbal U et al., Br J Pharmacol 160: 1016-28 (2010); Asano R et al., FEBS J 280: 4816-26 (2013); Gil D, Schrum A, Adv Biosci Biotechnol 4: 73-84 (2013)).

[0265] The skilled worker can engineer multimeric immunoconjugates of the present invention using various scFv-based polypeptide interactions known in the art, such as, e.g., scFv- based dimeric, trimeric, tetrameric complexes, etc. For example, the length of the linker in the scFv can affect the spontaneous assembly of non-covalent based, multimeric, multivalent structures. Generally, linkers of twelve amino acids or less, including the absence of any linker, promote the multimerization of polypeptides or proteins comprising scFvs into higher molecular weight species via favoring intermolecular domain swapping over intra- chain domain pairing (see e.g., Dolezal O et al., Protein Eng 16: 47- 56 (2003)). However, scFvs with no linker at all or alinker with an exemplary length of 15 amino acid residues may multimerize (Whitlow M et al., Protein Eng 6: 989-95 (1993); Desplancq D et al., Protein Eng T. 1027-33 (1994); Whitlow M et al., Protein Eng 7, 1017-26 (1994); Alfthan K et al., Protein Eng 8: 725-31 (1995)). The skilled worker can identify the multimeric structure(s) created and / or purified using techniques known in the art and / or described herein.

[0266] In some embodiments, amino acid sequence variants of the immunoconjugates described herein are contemplated. For example, it may be desirable to improve the binding affinity, stability, and / or other biological properties of the immunoconjugate of the present invention (e.g., alter the half-life or therapeutic window, reduce immunogenicity, or increase ease of manufacturing). Amino acid sequence variants of an immunoconjugate may be prepared by introducing appropriate modifications into the nucleotide sequence encoding the immunoconjugate, or by synthesis of the desired immunoconjugate or polypeptide. Such modifications include, for example, fusion of immunoglobulin domains or polypeptide sequences; substitution of hinge, linker(s), and / or chelator components; substitution of radioisotope. Such modifications include, for example, deletions from, and / or insertions into and / or substitutions of residues within the amino acid sequences of the immunoconjugate. Any combination of fusion, deletion, insertion, and substitution can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics, e.g., a certain binding affinity level of antigen binding, a certain level of KD, and / or a certain level of Koff

[0267] Antigen binding antibody fragments and sets of CDRs are provided herein. Such fragments may be truncated at the N-terminus or C-terminus, or may lack internal residues, for example, when compared with a full-length native antibody (e.g., a full-length cam elid VHH IgG2 or IgG3). Certain fragments may lack amino acid residues or domain that are not essential for a desired biological activity of the antibody or to reduce the total size of the immunoconjugate of the invention.

[0268] In some embodiments, a variant of an immunoconjugate of the present invention is made to be larger by the incorporation of additional structure. In some embodiments, an immunoconjugate is linked to a heterologous moiety or readily detectable moiety. In some further embodiments, the linkage comprises a proteinaceous fusion. In some further embodiments, the heterologous moiety is a cytotoxic agent. In some embodiments, a carboxy -terminal lysine residue is added to provide a site-specific attachment site. Amino acid sequence insertions include amino- and / or carboxyl-terminal fusions ranging in length from one residue to polypeptides containing a hundred or more residues, as well as intrasequence insertions of single or multiple amino acid residues. Examples of terminal insertions include an immunoconjugate with an N-terminalmethionyl residue. Other insertional variants of the immunoconjugate molecule include the fusion to the N- or C-terminus of the immunoconjugate to an enzyme (e.g., for ADEPT) or a polypeptide which increases the serum half-life of the immunoconjugate.

[0269] Nucleic acids that encode the immunoconjugate of the invention may be modified to produce chimeric or fusion immunoconjugate polypeptides, for example, by substituting human heavy chain and light chain constant domain (CH and CO sequences for the homologous murine sequences (U.S. Pat. No. 4,816,567; and Morrison, et al., Proc Natl Acad Set USA 81 : 6851 (1984)), or by fusing the immunoglobulin coding sequence with all or part of the coding sequence for a non-immunoglobulin polypeptide (heterologous polypeptide). The non-immunoglobulin polypeptide sequences can substitute for the constant domains of an immunoconjugate, or they are substituted for the variable domains of one antigen-combining site of an immunoconjugate to create a chimeric bivalent immunoconjugate comprising one antigen-combining site having specificity for an antigen and another antigen-combining site having specificity for a different antigen.

[0270] Variations in the antibody constructs used as antigen binding domains in the inventions described herein, can be made, for example, using any of the techniques and guidelines for conservative and non-conservative mutations set forth, for instance, in U.S. Patent No. 5,364,934. Variations may be a substitution, deletion or insertion of one or more codons encoding the immunoconjugate or polypeptide that results in a change in the amino acid sequence as compared with the native sequence antibody or polypeptide. Optionally the variation is by substitution of at least one amino acid with any other amino acid in one or more of the domains of the immunoconjugate. Guidance in determining which amino acid residue may be inserted, substituted or deleted without adversely affecting the desired activity may be found by comparing the sequence of the immunoconjugate with that of homologous known protein molecules and minimizing the number of amino acid sequence changes made in regions of high homology. Amino acid substitutions can be the result of replacing one amino acid with another amino acid having similar structural and / or chemical properties, such as the replacement of a leucine with a serine, i.e., conservative amino acid replacements. Insertions or deletions may optionally be in the range of about 1 to 5 amino acids. The variation allowed may be determined by systematically making insertions, deletions or substitutions of amino acids in the sequence and testing the resulting variants for activity exhibited by the full-length or mature native sequence.

[0271] In particular embodiments, conservative substitutions of interest are shown in Tables B and C, including under the heading of preferred substitutions. If such substitutions result in a change in biological activity, then more substantial changes, denominated exemplarysubstitutions in Table D, or as further described below in reference to amino acid classes, are introduced and the products screened.Table D

[0272] Substantial modifications in function or immunological identity of an immunoconjugate of the invention are accomplished by selecting substitutions that differ significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain. Naturally occurring residues are divided into groups based on common side-chain properties:(1) hydrophobic: norleucine, met, ala, val, leu, ile;(2) neutral hydrophilic: cys, ser, thr;(3) acidic: asp, glu;(4) basic: asn, gin, his, lys, arg;(5) residues that influence chain orientation: gly, pro; and(6) aromatic: trp, tyr, phe.

[0273] Non-conservative substitutions will entail exchanging a member of one of these classes for another class. Such substituted residues also may be introduced into the conservative substitution sites or, more preferably, into the remaining (non-conserved) sites.

[0274] The variations can be made using methods known in the art, such as, e.g., oligonucleotide-mediated (site-directed) mutagenesis, alanine scanning, and PCR mutagenesis. Site-directed mutagenesis (Carter et al., Nucl. Acids Res., 13: 4331 (1986); Zoller et al., Nucl. Acids Res., 10: 6487 (1987)), cassette mutagenesis (Wells et al., Gene, 34: 315 (1985)), restriction selection mutagenesis (Wells et al., Philos. Trans. R. Soc. London SerA, 317: 415 (1986)) or other known techniques can be performed on the cloned DNA to produce DNA molecules encoding an immunoconjugate variant of the invention.

[0275] In some embodiments, immunoconjugate variants having one or more amino acid substitutions are provided. Sites of interest for substitutional mutagenesis include the HVRs and FRs of immunoglobulin variable domains as well as within the immunoglobulin constant domains. Amino acid substitutions may be introduced into an immunoconjugate of interest and the products screened for a desired activity, e.g., improved / retained antigen binding, decreased / retained immunogenicity, improved / retained antibody-dependent cellular cytotoxicity (ADCC), improved / retained complement dependent cytotoxicity (CDC), improved / retained target inhibition, and / or improved / retained antibody-dependent cell-mediated phagocytosis (ADCP). Similarly, amino acid substitutions may be introduced into an immunoconjugate of interest and the products screened for the reduction or elimination of an activity, e.g., ADCC, CDC, target inhibition, and / or ADCP.

[0276] One type of substitutional variant involves substituting one or more hypervariable region residues of a parent antibody (e.g., a humanized or human antibody). Generally, the resulting variant(s) selected for further study will have modifications (e.g., improvements) in certain biological properties (e.g., increased affinity, reduced immunogenicity) relative to the parent antibody and / or will have substantially retained certain biological properties of the parent antibody. An illustrative substitutional variant is an affinity-matured antibody, which may be conveniently generated, e.g., using phage display-based affinity maturation techniques such as those described herein. Briefly, one or more HVR residues are mutated and the variant antibodies displayed on phage and screened for a particular biological activity (e.g., binding affinity).

[0277] Alterations (e.g., substitutions) may be made in HVRs, e.g., to improve immunoconjugate affinity. Such alterations may be made in HVR “hotspots,” i.e., residues encoded by codons that undergo mutation at high frequency during the somatic maturation process (see e.g., Chowdhury, Methods Mol. Biol. 207: 179-196 (2008)), and / or SDRs (a-CDRs), with the resulting variant VH or VL being tested for binding affinity. Affinity maturation by constructing and reselecting from secondary libraries has been described, e.g., in Hoogenboom et al. in Methods in Molecular Biology 178: 1-37 (O’Brien et al., ed., Human Press, Totowa, NJ, (2001)). In some embodiments of affinity maturation, diversity is introduced into the variable genes chosen for maturation by any of a variety of methods (e.g., error-prone PCR, chain shuffling, or oligonucleotide-directed mutagenesis). A secondary library is then created. The library is then screened to identify any antibody variants with the desired affinity. Another method to introduce diversity involves HVR-directed approaches, in which several HVR residues (e.g., 4-6 residues at a time) are randomized. HVR residues involved in antigen binding may be specifically identified, e.g., using alanine scanning mutagenesis or modeling. CDR-H3 and CDR-L3 in particular are often targeted.

[0278] In some embodiments, substitutions, insertions, or deletions may occur within one or more HVRs so long as such alterations do not substantially reduce the ability of the immunoconjugate to bind antigen. For example, conservative alterations (e.g., conservative substitutions as provided herein) that do not substantially reduce binding affinity may be made in HVRs. Such alterations may be outside of HVR “hotspots” or SDRs. In some embodiments of the variant VH and VL sequences provided above, each HVR either is unaltered, or contains no more than one, two or three amino acid substitutions.

[0279] A useful method for identification of residues or regions of an antibody that may be targeted for mutagenesis is called “alanine scanning mutagenesis” as described by Cunningham and Wells (1989) Science, 244: 1081-1085. In this method, a residue or group of target residues (e.g., charged residues such as Arg, Asp, His, Lys, and Glu) are identified and replaced by a neutral or negatively charged amino acid (e.g., alanine or polyalanine) to determine whether the interaction of the antibody with antigen is affected. Further substitutions may be introduced at the amino acid locations demonstrating functional sensitivity to the initial substitutions.

[0280] Alternatively, or additionally, a crystal structure of an antigen-antibody complex to identify contact points between the antibody and antigen. Such contact residues and neighboring residues may be targeted or eliminated as candidates for substitution. Variants may be screened to determine whether they contain the desired properties.

[0281] In some embodiments, the immunoconjugate of the present invention comprises an antibody construct (used as an antigen binding region herein) comprising a humanized immunoglobulin domain(s).

[0282] Humanized forms of non-human (e.g., camelid, murine, or rabbit) antibodies are chimeric immunoglobulins, immunoglobulin chains or fragments thereof (such as Fv, Fab, Fab', F(ab')2 or other antigen-binding subsequences of antibodies) which contain minimal sequence derived from non-human immunoglobulin. Humanized antibodies include human immunoglobulins (recipient antibody) in which residues from a complementary determining region (CDR) of the recipient are replaced by residues from a CDR of a non-human species (donor antibody) such as a camelid, mouse, rat or rabbit having the desired specificity, affinity and capacity. In some instances, Fv framework residues of the human immunoglobulin are replaced by corresponding non-human residues. Humanized antibodies may also comprise residues which are found neither in the recipient antibody nor in the imported CDR or framework sequences. In general, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin consensus sequence. The humanized antibody may also comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin (Jones et al., Nature, 321 : 522-5 (1986); Riechmann et al., Nature, 332: 323-9 (1988); and Presta, Curr. Op. Struct. Biol., 2: 593-6 (1992)).

[0283] Methods for humanizing non-human antibodies are well known in the art. Generally, a humanized antibody has one or more amino acid residues introduced into it from a source which is non-human. These non-human amino acid residues are often referred to as “import” residues, which are typically taken from an “import” variable domain. Humanization can be essentially performed following the method of Winter and co-workers (Jones et al., Nature, 321 :522-525 (1986); Riechmann et al., Nature, 332:323-327 (1988); Verhoeyen et al., Science, 239: 1534-1536 (1988)), by substituting rodent CDRs or CDR sequences for the corresponding sequences of a human antibody. Accordingly, such “humanized” antibodies are chimeric antibodies (U.S. Pat. No. 4,816,567), wherein substantially less than an intact human variable domain has been substituted by the corresponding sequence from a non-human species. In practice, humanized antibodies are typically human antibodies in which some CDR residues and possibly some FR residues are substituted by residues from analogous sites in rodent antibodies.

[0284] According to another method, antigen binding may be restored during humanization of antibodies through the selection of repaired hypervariable regions (see, e.g., US applicationSer. No. 11 / 061,841, filed Feb. 18, 2005). The method includes incorporating non-human hypervariable regions onto an acceptor framework and further introducing one or more amino acid substitutions in one or more hypervariable regions without modifying the acceptor framework sequence. Alternatively, the introduction of one or more amino acid substitutions may be accompanied by modifications in the acceptor framework sequence.

[0285] Any cysteine residue not involved in maintaining the proper conformation of the immunoconjugate of the invention also may be substituted, generally with serine, to improve the oxidative stability of the molecule and prevent aberrant crosslinking. Conversely, cysteine bond(s) may be added to the immunoconjugate of the invention to improve its stability (particularly where the antibody is an antibody fragment such as an Fv fragment or VHH fragment).

[0286] In some embodiments, it may be desirable to create cysteine engineered immunoconjugates in which one or more residues of an immunoconjugate are substituted with cysteine residues. In some embodiments, the substituted residues occur at accessible sites of the immunoconjugate. By substituting those residues with cysteine, reactive thiol groups are thereby positioned at accessible sites of the immunoconjugate and may be used to conjugate the immunoconjugate to other moieties, such as drug moieties or linker-drug moieties. In some embodiments, any one or more of the following residues may be substituted with cysteine: V205 (Kabat numbering) of the light chain; Al 18 (EU numbering) of the heavy chain; and S400 (EU numbering) of the heavy chain Fc region. Cysteine engineered antibodies may be generated as described, e.g., in US 7,521,541.

[0287] The skilled worker will appreciate that amino acid changes may alter post-translational processes of the immunoconjugate, such as changing the number or position of glycosylation sites or altering the membrane anchoring characteristics.

[0288] In some embodiments, an immunoconjugate provided herein is altered to increase or decrease the extent to which the immunoconjugate is glycosylated and / or to change the glycosylation pattern. “Altering the native glycosylation pattern” is intended for purposes herein to mean deleting one or more carbohydrate moieties found in a parental immunoconjugate of the invention (either by removing the underlying glycosylation site or by deleting the glycosylation by chemical and / or enzymatic means), and / or adding one or more glycosylation sites that are not present in the native sequence immunoconjugate of the invention. In addition, the phrase includes qualitative changes in the glycosylation of the native proteins, involving a change in the nature and proportions of the various carbohydrate moieties present.

[0289] Glycosylation of antibodies and other polypeptides is typically either N-linked or O- linked. N-linked refers to the attachment of the carbohydrate moiety to the side chain of anasparagine residue. The tripeptide sequences asparagine-X-serine and asparagine-X-threonine, where X is any amino acid except proline, are the recognition sequences for enzymatic attachment of the carbohydrate moiety to the asparagine side chain. Thus, the presence of either of these tripeptide sequences in a polypeptide creates a potential glycosylation site. O-linked glycosylation refers to the attachment of one of the sugars N-aceylgalactosamine, galactose, or xylose to a hydroxyamino acid, most commonly serine or threonine, although 5-hydroxyproline or 5- hydroxylysine may also be used.

[0290] Addition or deletion of glycosylation sites to an immunoconjugate may be conveniently accomplished by altering the amino acid sequence such that one or more glycosylation sites is created or removed. Addition of glycosylation sites to the immunoconjugate of the invention is conveniently accomplished by altering the amino acid sequence such that it contains one or more of the above-described tripeptide sequences (for N-linked glycosylation sites). The alteration may also be made by the addition of, or substitution by, one or more serine or threonine residues to the sequence of the original immunoconjugate of the invention (for O- linked glycosylation sites). The immunoconjugate of the invention amino acid sequence may optionally be altered through changes at the DNA level, particularly by mutating the DNA encoding the immunoconjugate of the invention at preselected bases such that codons are generated that will translate into the desired amino acids.

[0291] Where the immunoconjugate comprises an Fc region, the carbohydrate attached thereto may be altered. Native antibodies produced by mammalian cells typically comprise a branched, biantennary oligosaccharide that is generally attached by an N-linkage to Asn297 of the CH2 domain of the Fc region (see e.g., Wright et al. TIBTECH 15:26-32 (1997)). The oligosaccharide may include various carbohydrates, e.g., mannose, N-acetyl glucosamine (GlcNAc), galactose, and sialic acid, as well as a fucose attached to a GlcNAc in the “stem” of the biantennary oligosaccharide structure. In some embodiments, modifications of the oligosaccharide in an immunoconjugate of the invention may be made in order to create immunoconjugate variants with certain improved properties.

[0292] Another means of increasing the number of carbohydrate moieties on the immunoconjugate of the invention is by chemical or enzymatic coupling of glycosides to the polypeptide. Such methods are described in the art, e.g., in WO 87 / 05330 published 11 September 1987, and in Aplin and Wriston, CRC Crit. Rev. Biochem., pp. 259-306 (1981).

[0293] Removal of carbohydrate moieties present on the immunoconjugate of the invention may be accomplished chemically or enzymatically or by mutational substitution of codons encoding for amino acid residues that serve as targets for glycosylation. Chemical deglycosylationtechniques are known in the art and described, for instance, by Hakimuddin, et al., Arch. Biochem. Biophys., 259:52 (1987) and by Edge et al., Anal. Biochem., 118: 131 (1981). Enzymatic cleavage of carbohydrate moieties on polypeptides can be achieved by the use of a variety of endo- and exo-glycosidases as described by Thotakura et al., Meth. Enzymol., 138:350 (1987).

[0294] In some embodiments, immunoconjugate variants are provided having a carbohydrate structure that lacks fucose attached (directly or indirectly) to an Fc region. For example, the amount of fucose in such immunoconjugate may be from 1% to 80%, from 1% to 65%, from 5% to 65% or from 20% to 40%. The amount of fucose is determined by calculating the average amount of fucose within the sugar chain at Asn297, relative to the sum of all glycostructures attached to Asn297 (e.g., complex, hybrid and high mannose structures) as measured by MALDI- TOF mass spectrometry, as described in WO 2008 / 077546, for example. Asn297 refers to the asparagine residue located at about position 297 in the Fc region (Eu numbering of Fc region residues); however, Asn297 may also be located about ± 3 amino acids upstream or downstream of position 297, z.e., between positions 294 and 300, due to minor sequence variations in antibodies. Such fucosylation variants may have improved ADCC function (see e.g., US 2003 / 0157108; US 2004 / 0093621). Examples of publications related to “defucosylated” or “fucose-deficient” antibody variants include: US 2003 / 0157108; WO 2000 / 61739; WO 2001 / 29246; US 2003 / 01 15614; US 2002 / 0164328; US 2004 / 0093621; US 2004 / 0132140; US 2004 / 01 10704; US 2004 / 01 10282; US 2004 / 0109865; WO 2003 / 0851 19; WO 2003 / 084570; WO 2005 / 035586; WO 2005 / 035778; W02005 / 053742; W02002 / 031 140; Okazaki et al. J. Mol. Biol. 336: 1239-1249 (2004); Yamane-Ohnuki et al. Biotech. Bioeng. 87:614 (2004). Examples of cell lines capable of producing defucosylated antibodies include Lecl3 CHO cells deficient in protein fucosylation (Ripka et al. Arch. Biochem. Biophys. 249:533-545 (1986); US 2003 / 0157108; WO 2004 / 056312, Adams et al., especially at Example 11), and knockout cell lines, such as alpha- 1,6-fucosyltransferase gene, FUT8, knockout CHO cells (see e.g., Yamane- Ohnuki et al. Biotech. Bioeng. 87: 614 (2004); Kanda, Y. et al., Biotechnol. Bioeng., 94(4):680- 688 (2006); W02003 / 085107)).

[0295] Immunoconjugate variants are further provided with bisected oligosaccharides, e.g., in which a biantennary oligosaccharide attached to the Fc region of the antibody is bisected by GlcNAc. Such immunoconjugate variants may have reduced fucosylation and / or improved ADCC function. Examples of such antibody variants are described, e.g., in WO 2003 / 011878; US 6,602,684; US 2005 / 0123546. Immunoconjugate variants with at least one galactose residue in the oligosaccharide attached to the Fc region are also provided. Such immunoconjugate variantsmay have improved CDC function. Such antibody variants are described, e.g., in WO 1997 / 030087; WO 1998 / 058964; and WO 1999 / 022764.Immunoconj ugate Derivatives and Other Modifications

[0296] Covalent modifications of the immunoconjugates of the invention are included within the scope of this invention. One type of covalent modification includes reacting targeted amino acid residues of an immunoconjugate of the invention with an organic derivatizing agent that is capable of reacting with selected side chains or the N- or C- terminal residues of the immunoconjugate. Derivatization with bifunctional agents is useful, for instance, for crosslinking an immunoconjugate of the invention to a water-insoluble support matrix or surface for use in the method for purifying the immunoconjugates of the invention, and vice-versa. Commonly used crosslinking agents include, e.g., l,l-bis(diazoacetyl)-2-phenylethane, glutaraldehyde, N- hydroxysuccinimide esters, for example, esters with 4-azidosalicylic acid, homobifunctional imidoesters, including disuccinimidyl esters such as 3,3'-dithiobis(succinimidylpropionate), bifunctional maleimides such as bis-N-maleimido-l,8-octane and agents such as methyl-3-[(p- azidophenyl)dithio]propioimidate.

[0297] Other modifications include deamidation of glutaminyl and asparaginyl residues to the corresponding glutamyl and aspartyl residues, respectively, hydroxylation of proline and lysine, phosphorylation of hydroxyl groups of seryl or threonyl residues, methylation of the a-amino groups of lysine, arginine, and histidine side chains (T.E. Creighton, Proteins: Structure and Molecular Properties, W.H. Freeman & Co., San Francisco, pp. 79-86 (1983)), acetylation of the N-terminal amine, and amidation of any C-terminal carboxyl group.

[0298] In some embodiments, an immunoconjugate provided herein may be further modified to contain additional nonproteinaceous moieties that are known in the art and readily available. The moieties suitable for derivatization of the immunoconjugate include but are not limited to water soluble polymers. Non-limiting examples of water soluble polymers include, but are not limited to, polyethylene glycol (PEG), copolymers of ethylene glycol / propylene glycol, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinyl pyrrolidone, poly-1, 3 -di oxolane, poly-1, 3,6-trioxane, ethylene / maleic anhydride copolymer, polyaminoacids (either homopolymers or random copolymers), and dextran or poly(n-vinyl pyrrolidone) polyethylene glycol, propropylene glycol homopolymers, proly propylene oxide / ethylene oxide co-polymers, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, and mixtures thereof. Polyethylene glycol propionaldehyde may have advantages in manufacturing due to its stability in water. The polymer may be of any molecular weight, and may be branched or unbranched. The number of polymers attached to the immunoconjugate may vary, and if more than one polymer are attached,they can be the same or different molecules. In general, the number and / or type of polymers used for derivatization can be determined based on considerations including, but not limited to, the particular properties or functions of the immunoconjugate to be improved, whether the immunoconjugate derivative will be used in a therapy under defined conditions, etc.

[0299] PEG derivatized immunoconjugates of the invention may comprise linkers comprising one or more -CH2CH2O- and can be used to alter biodistribution and pharmacokinetics of the immunoconjugate. PEGs can be prepared in a polymeric form or as discrete oligomers. Bifunctionalized versions of these polymers can link immunoconjugates with a chelating agent and / or provide additional size and / or solubility to the overall molecule. In some embodiments, the PEG derivatized immunoconjugates exhibit reduced immunogenicity compared to their un- derivatized parental molecules.Methods of Producing the Immunoconjugates of the Present Invention

[0300] The present invention provides a composition comprising one or more of the immunoconjugates according to any of the above embodiments or described herein. In another aspect, the invention provides an isolated nucleic acid encoding a radioisotope delivering platform as described herein. Also provided herein are nucleic acids encoding the protein components of the immunoconjugates of the present invention, expression vectors comprising the aforementioned nucleic acid, and host cells comprising the aforementioned expression vectors.

[0301] In another aspect, the invention provides a host cell comprising a nucleic acid and / or vector as provided herein. In some embodiments, the host cell of the present invention is isolated or purified. In some embodiments, the host cell of the present invention is in a cell culture medium. The nucleic acids, expression vectors, and host cells of the invention may be used to produce a composition comprising one or more of the immunoconjugates of the invention. In some embodiments, the host cell is eukaryotic. In some embodiments, the host cell is mammalian. In some embodiments, the host cell is a Chinese Hamster Ovary (CHO) cell. In some embodiments, the host cell is prokaryotic. In some embodiments, the host cell is E. coli.

[0302] A description follows as to illustrative techniques for the production of the immunoconjugates and radioimmunoconjugates of the present invention for use in accordance with the methods of the present invention. In some embodiments, the invention provides a process for making an immunoconjugate of the present invention, the method comprising culturing a host cell as provided herein under conditions suitable for the expression vector encoding the radioisotope delivery platform and recovering or purifying the radioisotope delivery platform. In some embodiments, the method further comprises radiolabeling the radioisotope delivery platform with an appropriate isotope, such as, e.g., an alpha or beta particle emitter.Generation and Identification of Antigen Binding Domains, Immunoconjugates and NucleicAcids

[0303] Antigen binding domains useful as antigen binding regions herein may be identified in antibodies that are either monoclonal antibodies and / or polyclonal antibodies. DNA encoding a monoclonal antibody is readily isolated and sequenced using conventional procedures. Once isolated, the DNA may be placed into expression vectors, which are then transfected into host cells such as E. coli cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce antibody protein, to obtain the synthesis of monoclonal antibodies in the recombinant host cells (see e.g., Skerra et al., Curr. Opinion in Immunol., 5:256- 262 (1993) and Pltickthun, Immunol Revs. 130: 151-188 (1992)).

[0304] In some embodiments, the antigen binding domains of an immunoconjugate of the present invention, or fragments thereof, are isolated by screening phage libraries containing phage that display various fragments of antibody variable region (Fv, scFv, or VHH) fused to phage coat protein. Such phage libraries are screened for binding to the desired target antigen or epitope. Clones expressing Fv fragments, scFv’s, or VHH’s capable of binding to the desired antigen are adsorbed to the antigen and thus separated from the non-binding clones in the library. The binding clones are then eluted from the antigen, and can be further enriched by additional cycles of antigen adsorption / elution.

[0305] In some embodiments, the antibody or antibody fragments thereof are isolated from antibody phage libraries generated using the techniques described in McCafferty et al., Nature, 348:552-554 (1990). Clackson et al., Nature, 352:624-628 (1991) and Marks et al., J Mol Biol., 222:581-597 (1991) describe the isolation of murine and human antibodies, respectively, using phage libraries. Subsequent publications describe the production of high affinity (nM range) human antibodies by chain shuffling (Marks et al., Bio / Technology, 10:779-783 (1992)), as well as combinatorial infection and in vivo recombination as a strategy for constructing very large phage libraries (Waterhouse et al., Nuc Acids Res. 21 :2265-2266 (1993)). Variable domains can be displayed functionally on phage, either as single-chain Fv (scFv) fragments, in which VH and VL are covalently linked through a short, flexible peptide, or as Fab fragments, in which they are each fused to a constant domain and interact non-covalently, as described in Winter et al., Ann. Rev. Immunol., 12: 433-455 (1994).

[0306] Repertoires of VH and VL genes can be separately cloned by polymerase chain reaction (PCR) and recombined randomly in phage libraries, which can then be searched for antigen binding clones as described in Winter et al., Ann. Rev. Immunol., 12: 433-455 (1994). Naive libraries for screening can be constructed from non-immunized sources to provide high-affinity antibodies to antigens (see e.g., Griffiths et al., EMBO J, 12: 725-734 (1993)). Another example is naive libraries constructed synthetically by cloning the unrearranged V-gene segments from stem cells, and using PCR primers containing random sequence to encode the highly variable CDR3 regions and to accomplish rearrangement in vitro as described by Hoogenboom and Winter, J. Mol. Biol., 227: 381-388 (1992).

[0307] Screening of the libraries can be accomplished by various techniques known in the art. For example, target antigen can be used to coat the wells of adsorption plates, expressed on host cells affixed to adsorption plates or used in cell sorting, or conjugated to biotin for capture with streptavidin-coated beads, or used in any other method for panning display libraries. The selection of antibodies with slow dissociation kinetics (and strong binding affinities) can be promoted by use of long washes and monovalent phage display as described in Bass et al., Proteins, 8: 309- 314 (1990) and in WO 1992 / 09690, and a low coating density of antigen as described in Marks et aL, Biotechnol., 10: 779-783 (1992).

[0308] Techniques for screening a cDNA library are well known in the art. Libraries can be screened with probes (such as oligonucleotides of at least about 20-80 bases) designed to identify the gene of interest or the protein encoded by it. Screening the cDNA or genomic library with the selected probe may be conducted using standard procedures, such as described in Sambrook et al., Molecular Cloning: A Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989). An alternative means to isolate the gene encoding immunoconjugate of the invention is to use PCR methodology (Sambrook et al., supra, Dieffenbach et al., PCR Primer: A Laboratory Manual (Cold Spring Harbor Laboratory Press, 1995)).

[0309] DNA encoding an immunoconjugate of the invention may be obtained from a cDNA library prepared from tissue believed to possess the immunoconjugate of the invention mRNA and to express it at a detectable level. Accordingly, human immunoconjugate of the invention DNA can be conveniently obtained from a cDNA library prepared from human tissue. The immunoconjugate of the invention-encoding gene may also be obtained from a genomic library or by known synthetic procedures (e.g., automated nucleic acid synthesis). For some embodiments, desired polynucleotide sequences encoding antibodies may be isolated and sequenced from antibody producing cells such as hybridoma cells.

[0310] Sequences identified in such library screening methods can be compared and aligned to other known sequences deposited and available in public databases such as GenBank or other private sequence databases. Sequence identity (at either the amino acid or nucleotide level) within defined regions of the molecule or across the full-length sequence can be determined using methods known in the art and as described herein. Any of the antibody CDRs or heavy chainvariable fragments of the present invention can be obtained by designing a suitable antigen screening procedure to select for the phage clone of interest followed by construction of an antibody clone using the variable domain and / or CDRs sequences from a phage clone of interest and suitable constant region (Fc) sequences described in Kabat et al., 1991, supra.Immunoconjugate Production; Host Cells and Expression Vectors of the Invention

[0311] The description below relates primarily to production of the antibody constructs of the invention by culturing cells transformed or transfected with a vector-containing immunoconjugate of the invention-encoding nucleic acid. It is, of course, contemplated that alternative methods, which are well known in the art, may be employed to prepare the antibody constructs of the invention. For instance, the appropriate amino acid sequence, or portions thereof, may be produced by direct peptide synthesis using solid-phase techniques (e.g., Stewart et al., Solid-Phase Peptide Synthesis, W.H. Freeman Co., San Francisco, CA (1969); Merrifield, J, Am. Chem. Soc., 85: 2149-54 (1963)). In vitro protein synthesis may be performed using manual techniques or by automation. Automated synthesis may be accomplished, for instance, using an Applied Biosystems Peptide Synthesizer (Foster City, CA) using manufacturer’s instructions. Various portions of the immunoconjugate of the invention may be chemically synthesized separately and combined using chemical or enzymatic methods to produce the desired immunoconjugate of the invention.

[0312] Antibody constructs may be produced using recombinant methods and compositions, e.g., as described in US 4,816,567. In one embodiment, isolated nucleic acid encoding an antibody described herein is provided. Such nucleic acid may encode an amino acid sequence comprising the VH of the antibody and / or comprising the VL amino acid sequence (e.g., the light and / or heavy chains of the antibody). In a further embodiment, one or more vectors (e.g., expression vectors) comprising such nucleic acid are provided. In a further embodiment, a host cell comprising such nucleic acid is provided. In some embodiments, a host cell comprises (e.g., has been transformed with): (1) a vector comprising a nucleic acid that encodes an amino acid sequence comprising the VH of the antibody. In some other embodiments, a host cell comprises: (1) a vector comprising a nucleic acid that encodes an amino acid sequence comprising the VL of the antibody and an amino acid sequence comprising the VH of the antibody, or (2) a first vector comprising a nucleic acid that encodes an amino acid sequence comprising the VL of the antibody and a second vector comprising a nucleic acid that encodes an amino acid sequence comprising the VH of the antibody. In one embodiment, the host cell is eukaryotic, e.g., a Chinese Hamster Ovary (CHO) cell or lymphoid cell (e.g., YO, NSO, Sp20 cell). In one embodiment, a method of making an immunoconjugate of the invention is provided, wherein the method comprises culturing a hostcell comprising a nucleic acid encoding the antibody, as provided above, under conditions suitable for expression of the antibody, and optionally recovering the antibody from the host cell (or host cell culture medium).

[0313] For recombinant production of an immunoconjugate of the present invention, nucleic acid encoding an antibody construct, e.g., as described above, is isolated and inserted into one or more vectors for further cloning and / or expression in a host cell. Such nucleic acid may be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and / or light chains of the antibody). Nucleic acid molecules encoding amino acid sequence of the immunoconjugate of the present invention (including sequence variants) may be prepared by a variety of methods known to the skilled worker. These methods include, but are not limited to, isolation from a natural source (in the case of naturally occurring amino acid sequence variants) or preparation by oligonucleotide-mediated (or site-directed) mutagenesis, PCR mutagenesis, and cassette mutagenesis of an earlier prepared variant or a non-variant version of the antibody construct.Manipulation of Host Cells for Immunoconjugate Production

[0314] Host cells are transfected or transformed with expression or cloning vectors described herein for immunoconjugate of the invention production and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences. The culture conditions, such as media, temperature, pH and the like, can be selected by the skilled artisan without undue experimentation. In general, principles, protocols, and practical techniques for maximizing the productivity of cell cultures can be found in Mammalian Cell Biotechnology: a Practical Approach, M. Butler, ed. (IR.L Press, 1991) and Sambrook et al., supra.

[0315] Suitable host cells for cloning or expression of immunoconjugate-encoding nucleic acids and vectors include prokaryotic or eukaryotic cells described herein. For example, antibodies may be produced in bacteria, in particular when glycosylation and Fc effector function are not needed. For expression of antibody fragments and polypeptides in bacteria, see e.g., US 5,648,237; US 5,789,199; US 5,840,523; and Charlton, Methods in Molecular Biology , Vol. 248 (B.K.C. Lo, ed., Humana Press, Totowa, NJ, 2003), pp. 245-254, describing expression of antibody fragments in E. colt). After expression, the immunoconjugate may be isolated from the bacterial cell paste in a soluble fraction and can be further purified.

[0316] In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for immunoconjugate-encoding vectors, including fungi and yeast strains whose glycosylation pathways have been “humanized,” resulting in the productionof an antibody with a partially or fully human glycosylation pattern (see e.g., Gerngross, Nat. Biotech. 22:1409-1414 (2004); Li et al., Nat. Biotech. 24:210-215 (2006)).

[0317] Suitable host cells for the expression of glycosylated immunoconjugate are also derived from multicellular organisms (e.g., invertebrates and vertebrates). Examples of invertebrate cells include plant and insect cells. Numerous baculoviral strains have been identified which suitable for use in conjunction with insect cells, particularly for transfection of Spodoptera frugiperda cells. Plant cell cultures can also be utilized as hosts (see e.g., US 5,959,177; US 6,040,498; US 6,420,548; US 7,125,978; and US 6,417,429.

[0318] Vertebrate cells may also be used as hosts. For example, mammalian cell lines that are adapted to grow in suspension may be useful. Other examples of useful mammalian host cell lines are monkey kidney CV 1 line transformed by SV40 (COS-7); human embryonic kidney line (293 or 293 cells as described, e.g., in Graham et al., J Gen Viral. 36:59 (1977); baby hamster kidney cells (BHK); mouse Sertoli cells (TM4 cells as described, e.g., in Mather, Biol. Reprod. 23:243- 251 (1980); monkey kidney cells (CV 1); African green monkey kidney cells (VERO-76); human cervical carcinoma cells (HELA); canine kidney cells (MOCK; buffalo rat liver cells (BRL 3A); human lung cells (W138); human liver cells (Hep 02); mouse mammary tumor (MMT 060562); TRI cells, as described, e.g., in Mather et al., Annals N. Y. Acad. Sci. 383:44-68 (1982); MRC 5 cells; and FS4 cells. Other useful mammalian host cell lines include Chinese hamster ovary (CHO) cells, including DHFK CHO cells (Urlaub et al., Proc Natl Acad Sci USA 77:4216 (1980)); and myeloma cell lines such as YO, NSO and Sp2 / 0. For a review of certain mammalian host cell lines suitable for immunoconjugate production, see e.g., Yazaki and Wu, Methods in Molecular Biology, Vol. 248 (B.K.C. Lo, ed., Humana Press, Totowa, NJ), pp. 255-268 (2003).

[0319] Methods of eukaryotic cell transfection and prokaryotic cell transformation, which means introduction of DNA into the host so that the DNA is replicable, either as an extrachromosomal or by chromosomal integrant, are known to the skilled worker, for example, CaCh, CaPO4, liposome-mediated, polyethylene-gycol / DMSO and electroporation. Depending on the host cell used, transformation is performed using standard techniques appropriate to such cells. The calcium treatment employing calcium chloride, as described in Sambrook et al., supra, or electroporation is generally used for prokaryotes. Infection with Agrobacterium tumefaciens is used for transformation of certain plant cells, as described by Shaw et al., Gene, 23:315 (1983) and WO 89 / 05859 published 29 June 1989. For mammalian cells without such cell walls, the calcium phosphate precipitation method of Graham and van der Eb, Virology, 52:456-457 (1978) can be employed. General aspects of mammalian cell host system transfections have been described in U.S. Patent No. 4,399,216. Transformations into yeast are typically carried outaccording to the method of Van Solingen et al., J. Bact., 130:946 (1977) and Hsiao et al., Proc Natl Acad Set USA 76:3829 (1979). However, other methods for introducing DNA into cells, such as by nuclear microinjection, electroporation, bacterial protoplast fusion with intact cells, or polycations, e.g., polybrene, polyomithine, may also be used. For various techniques for transforming mammalian cells, see Keown et al., Methods in Enzymology, 185:527-537 (1990) and Mansour et al., Nature, 336:348-352 (1988).Prokaryotic Host Cells

[0320] Suitable prokaryotes include but are not limited to archaebacteria and eubacteria, such as Gram-negative or Gram-positive organisms, for example, Enterobacteriaceae such as E. coli. Various E. coli strains are publicly available, such as K12 strain MM294 (ATCC 31,446); X1776 (ATCC 31,537); W3110 (ATCC 27,325) and K5 772 (ATCC 53,635). Other suitable prokaryotic host cells include Enterobacteriaceae such as Escherichia, e.g., E. coli, Enterobacter, Erwinia, Klebsiella, Proteus, Salmonella, e.g., Salmonella lyphimiiriiim, Serratia, e.g., Serratia marcescans, and Shigella, as well as Bacilli such as B. subtilis and B. licheniformis (e.g., B. licheniformis 41P disclosed in DD 266,710 published 12 April 1989), Pseudomonas such as P. aeruginosa, Rhizobia, Vitreoscilla, Paracoccus and Streptomyces. These examples are illustrative rather than limiting. E. coli strain W3110 is one advantageous host or parent host because it is a common host strain for recombinant DNA product fermentations. Preferably, the host cell secretes minimal amounts of proteolytic enzymes. For example, strain W3110 (Bachmann, Cellular and Molecular Biology, vol. 2 (Washington, D.C.: American Society for Microbiology, 1987), pp. 1190-1219; ATCC Deposit No. 27,325) may be modified to effect a genetic mutation in the genes encoding proteins endogenous to the host, with examples of such hosts including E. coli W3110 strain 1A2, which has the complete genotype tonA ; E. coli W3110 strain 9E4, which has the complete genotype tonA ptr3; E. coli W3110 strain 27C7 (ATCC 55,244), which has the complete genotype tonA ptr3 phoA E15 (argF-lac)169 degP ompT kanr; E. coli W3110 strain 37D6, which has the complete genotype tonA ptr3 phoA E15 (argF-lac)169 degP ompT rbs7 ilvG kanr; E. coli W3 110 strain 40B4, which is strain 37D6 with a non-kanamycin resistant degP deletion mutation; E. coli W3110 strain 33D3 having genotype W3110 AfhuA (AtonA) ptr3 lac Iq lacL8 AompTA(nmpc-fepE) degP41 kanR (U.S. Pat. No. 5,639,635) and an A. coli strain having mutant periplasmic protease disclosed in U.S. Patent No. 4,946,783 issued 7 August 1990. Other strains and derivatives thereof, such as E. coli 294 (ATCC 31,446), E. coli B, E. coli 1776 (ATCC 31,537) and A. coli RV308 (ATCC 31,608) are also suitable. These examples are illustrative rather than limiting. Methods for constructing derivatives of any of the above-mentioned bacteria having defined genotypes are known in the art and described in, for example, Bass et al., Proteins, 8:309-314 (1990). It is generally necessary to select the appropriate bacteria taking into consideration replicability of the replicon in the cells of a bacterium. For example, E. coli, Serratia, or Salmonella species can be suitably used as the host when well known plasmids such as pBR322, pBR325, pACYC177, or pKN410 are used to supply the replicon. Typically the host cell should secrete minimal amounts of proteolytic enzymes, and additional protease inhibitors may desirably be incorporated in the cell culture. Alternatively, in vitro methods of cloning, e.g., PCR or other nucleic acid polymerase reactions, are suitable.

[0321] Full length antibody, antibody fragments, and antibody fusion proteins can be produced in bacteria, in particular when glycosylation and Fc effector function are not needed. Full length antibodies have greater half-life in circulation. Production in A. coli is faster and more cost efficient. For expression of antibody fragments and polypeptides in bacteria, see, e.g., U.S. 5,648,237; U.S. 5,789,199 and U.S. 5,840,523, which describe translation initiation region (TIR) and signal sequences for optimizing expression and secretion. After expression, the immunoconjugate is isolated from the E. coli cell paste in a soluble fraction and can be purified through, e.g., a protein A or G column depending on the isotype. Final purification can be carried out similar to the process for purifying antibody expressed e.g., in CHO cells.Eukaryotic Host Cells

[0322] In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for immunoconjugate of the invention-encoding vectors. Saccharomyces cerevisiae is a commonly used lower eukaryotic host microorganism. Others include Schizosaccharomyces pombe (Beach and Nurse, Nature, 290: 140 (1981); EP 139,383 published 2 May 1985); Kluyveromyces hosts (U.S. Patent No. 4,943,529; Fleer et al., Bio / Technology, 9: 968-75 (1991)) such as, e.g., K. lactis (MW98-8C, CBS683, CBS4574; Louvencourt et al., J. Bacterio!., 154(2):737-742 (1983)), K. fragilis (ATCC 12,424), K. bulgaricus (ATCC 16,045), K. wickeramii (ATCC 24,178), K. waltii (ATCC 56,500), K. drosophilarum (ATCC 36,906; Van den Berg et al., Bio / Technology, 8: 135 (1990)), K. thermotolerans, and K. marxianus,' yarrowia (EP 402,226); Pichia pastoris (EP 183,070; Sreekrishna et al., J. Basic Microbiol., 28:265-278 (1988)); Candida; Trichoderma reesia (EP 244,234); Neurospora crassa (Case et al., Proc Natl Acad Sci USA 76:5259-5263 (1979)); Schwanniomyces such as Schwanniomyces occidentalis (EP 394,538 published 31 October 1990); and filamentous fungi such as, e.g., Neurospora, Penicillium, Tolypocladium (WO 91 / 00357 published 10 January 1991), and Aspergillus hosts such as A. nidulans (Ballance et al., Biochem. Biophys. Res. Commun., 112:284-289 (1983); Tilbum et al., Gene, 26:205-221 (1983); Yelton et al., Proc Natl Acad Sci USA 81 : 1470-1474 (1984)) and A. niger (Kelly and Hynes, EMBO J.,4:475-479 (1985)). Methylotropic yeasts are suitable herein and include, but are not limited to, yeast capable of growth on methanol selected from the genera consisting of Hansenula, Candida, Kloeckera, Pichia, Saccharomyces, Torulopsis, and Rhodotorula. A list of specific species that are exemplary of this class of yeasts may be found in C. Anthony, The Biochemistry of Methyl otrophs, 269 (1982).

[0323] Suitable host cells for the expression of glycosylated immunoconjugate of the invention are derived from multicellular organisms. Examples of invertebrate cells include insect cells such as Drosophila S2 and Spodoptera Sf9, as well as plant cells, such as cell cultures of cotton, corn, potato, soybean, petunia, tomato, and tobacco. Numerous baculoviral strains and variants and corresponding permissive insect host cells from hosts such as Spodoptera frugiperda (caterpillar), Aedes aegypti (mosquito), Aedes albopictus (mosquito), Drosophila melanogaster (fruit fly), and Bombyx mori have been identified. A variety of viral strains for transfection are publicly available, e.g., the L-l variant of Autographa californica NPV and the Bm-5 strain of Bombyx mori NPV, and such viruses may be used as the virus herein according to the present invention, particularly for transfection of Spodoptera frugiperda cells.

[0324] However, interest has been greatest in vertebrate cells, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure. Examples of useful mammalian host cell lines are monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol. 36:59 (1977)); baby hamster kidney cells (BHK, ATCC CCL 10); Chinese hamster ovary cells / -DHFR (CHO, Urlaub et al., Proc Natl Acad Sci USA 77:4216 (1980)); mouse sertoli cells (TM4, Mather, Biol. Reprod. 23:243-251 (1980)); monkey kidney cells (CV1 ATCC CCL 70); African green monkey kidney cells (VERO-76, ATCC CRL-1587); human cervical carcinoma cells (HELA, ATCC CCL 2); canine kidney cells (MDCK, ATCC CCL 34); buffalo rat liver cells (BRL 3 A, ATCC CRL 1442); human lung cells (W138, ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI cells (Mather et al., Annals N.Y. Acad. Sci. 383:44-68 (1982)); MRC 5 cells; FS4 cells; and a human hepatoma line (Hep G2).

[0325] Host cells are transformed with the above-described expression or cloning vectors for immunoconjugate of the invention production and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.Selection and Use of a Replicable Vector

[0326] For recombinant production of a radioisotope delivery platform of the invention, the nucleic acid (e.g., cDNA or genomic DNA) encoding it is isolated and inserted into a replicable vector for further cloning (amplification of the DNA) or for expression. DNA encoding the immunoconjugate is readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of an antibody). Many vectors are available. The choice of vector depends in part on the host cell to be used. Generally, suitable host cells are of either prokaryotic or eukaryotic (generally mammalian) origin.

[0327] The vector may, for example, be in the form of a plasmid, cosmid, viral particle, or phage. The appropriate nucleic acid sequence may be inserted into the vector by a variety of procedures. In general, DNA is inserted into an appropriate restriction endonuclease site(s) using techniques known in the art. Vector components generally include, but are not limited to, one or more of a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence. Construction of suitable vectors containing one or more of these components employs standard ligation techniques which are known to the skilled artisan.

[0328] The immunoconjugate of the invention may be produced recombinantly not only directly, but also as a fusion polypeptide with a heterologous polypeptide, which may be a signal sequence or other polypeptide having a specific cleavage site at the N-terminus of the mature protein or polypeptide. In general, the signal sequence may be a component of the vector, or it may be a part of the immunoconjugate of the invention-encoding DNA that is inserted into the vector. The signal sequence may be a prokaryotic signal sequence selected, for example, from the group of the alkaline phosphatase, penicillinase, Ipp, or heat-stable enterotoxin II leaders. For yeast secretion the signal sequence may be, e.g., the yeast invertase leader, alpha factor leader (including Saccharomyces and Kluyveromyces a-factor leaders, the latter described in U.S. Patent No. 5,010,182), or acid phosphatase leader, the C. albicans glucoamylase leader (EP 362,179 published 4 April 1990), or the signal described in WO 90 / 13646 published 15 November 1990. In mammalian cell expression, mammalian signal sequences may be used to direct secretion of the protein, such as signal sequences from secreted polypeptides of the same or related species, as well as viral secretory leaders.Culturing Host Cells Producing Radioisotope Delivery Platforms

[0329] The host cells used to produce the immunoconjugate of the invention of this invention may be cultured in a variety of media and culture conditions.Prokaryotic Host Cell Cultures

[0330] Prokaryotic cells used to produce the polypeptides of the invention are grown in media known in the art and suitable for culture of the selected host cells. Examples of suitable media include Luria broth (LB) plus necessary nutrient supplements. In some embodiments, the media also contains a selection agent, chosen based on the construction of the expression vector, to selectively permit growth of prokaryotic cells containing the expression vector. For example, ampicillin is added to media for growth of cells expressing ampicillin resistant gene.

[0331] Any necessary supplements besides carbon, nitrogen, and inorganic phosphate sources may also be included at appropriate concentrations introduced alone or as a mixture with another supplement or medium such as a complex nitrogen source. Optionally the culture medium may contain one or more reducing agents selected from the group consisting of glutathione, cysteine, cystamine, thioglycollate, di thioerythritol and dithiothreitol.

[0332] The prokaryotic host cells are cultured at suitable temperatures. For A. coli growth, for example, the preferred temperature ranges from about 20°C to about 39°C, more preferably from about 25°C to about 37°C, even more preferably at about 30°C. The pH of the medium may be any pH ranging from about 5 to about 9, depending mainly on the host organism. For E. coli, the pH is preferably from about 6.8 to about 7.4, and more preferably about 7.0.

[0333] If an inducible promoter is used in the expression vector of the invention, protein expression is induced under conditions suitable for the activation of the promoter. In one aspect of the invention, PhoA promoters are used for controlling transcription of the polypeptides. Accordingly, the transformed host cells are cultured in a phosphate-limiting medium for induction. In some embodiments, the phosphate-limiting medium is the C.R.A.P medium (see, e.g., Simmons et al., J. Immunol. Methods (2002), 263: 133-47). A variety of other inducers may be used, according to the vector construct employed, as is known in the art.

[0334] In one embodiment, the expressed polypeptides of the present invention are secreted into and recovered from the periplasm of the host cells. Protein recovery typically involves disrupting the microorganism, generally by such means as osmotic shock, sonication or lysis. Once cells are disrupted, cell debris or whole cells may be removed by centrifugation or filtration. The proteins may be further purified, for example, by affinity resin chromatography. Alternatively, proteins can be transported into the culture media and isolated therein. Cells may be removed from the culture and the culture supernatant being filtered and concentrated for further purification of the proteins produced. The expressed polypeptides can be further isolated and identified using commonly known methods such as polyacrylamide gel electrophoresis (PAGE) and Western blot assay.

[0335] In one aspect of the invention, immunoconjugate production is conducted in large quantity by a fermentation process. Various large-scale fed-batch fermentation procedures are available for production of recombinant proteins. Large-scale fermentations have at least 1000 liters of capacity, preferably about 1,000 to 100,000 liters of capacity. These fermentors use agitator impellers to distribute oxygen and nutrients, especially glucose (a preferred carbon / energy source). Small-scale fermentation refers generally to fermentation in a fermentor that is no more than approximately 100 liters in volumetric capacity, and can range from about 1 liter to about 100 liters.

[0336] In a fermentation process, induction of protein expression is typically initiated after the cells have been grown under suitable conditions to a desired density, e.g., an OD550 of about 180-220, at which stage the cells are in the early stationary phase. A variety of inducers may be used, according to the vector construct employed, as is known in the art and described above. Cells may be grown for shorter periods prior to induction. Cells are usually induced for about 12- 50 hours, although longer or shorter induction time may be used.

[0337] To improve the production yield and quality of the polypeptides of the invention, various fermentation conditions can be modified. For example, to improve the proper assembly and folding of the secreted immunoconjugate polypeptides, additional vectors overexpressing chaperone proteins, such as Dsb proteins (DsbA, DsbB, DsbC, DsbD and or DsbG) or FkpA (a peptidylprolyl cis, trans-isomerase with chaperone activity) can be used to co-transform the host prokaryotic cells. The chaperone proteins have been demonstrated to facilitate the proper folding and solubility of heterologous proteins produced in bacterial host cells. Chen et al. (1999) J Bio Chem 274: 19601-5; U.S. Patent No. 6,083,715; U.S. Patent No. 6,027,888; Bothmann and Pluckthun (2000) J. Biol. Chem. 275: 17100-5; Ramm and Pluckthun (2000) J. Biol. Chem. 275: 17106-13; Arie et al. (2001) Mol. Microbiol. 39: 199-210.

[0338] To minimize proteolysis of expressed heterologous proteins (especially those that are proteolytically sensitive), certain host strains deficient for proteolytic enzymes can be used for the present invention. For example, host cell strains may be modified to effect genetic mutation(s) in the genes encoding known bacterial proteases such as Protease III, OmpT, DegP, Tsp, Protease I, Protease Mi, Protease V, Protease VI and combinations thereof. Some E. coli protease-deficient strains are available and described in, for example, Joly et al. (1998), supra; U.S. Patent No. 5,264,365; U.S. Patent No. 5,508,192; Hara et al., Microbial Drug Resistance, 2 :63-72 (1996).

[0339] In one embodiment, E. coli strains deficient for proteolytic enzymes and transformed with plasmids overexpressing one or more chaperone proteins are used as host cells in the expression system of the invention.Eukaryotic Host Cell Cultures

[0340] Commercially available media such as Ham’s F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPMI-1640 (Sigma), and Dulbecco’s Modified Eagle Medium ((DMEM), Sigma) are suitable for culturing the host cells. In addition, any of the media described in Ham et al., Meth. Enz. 58: 44 (1979), Barnes et al., Anal. BiochemAQZ'. 255 (1980), U.S. Pat. Nos. 4,767,704; 4,657,866; 4,927,762; 4,560,655; or 5,122,469; WO 90 / 03430; WO 87 / 00195; or U.S. Patent Re. 30,985 may be used as culture media for the host cells. Any of these media may be supplemented as necessary with hormones and / or other growth factors (such as insulin, transferrin, or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such as HEPES), nucleotides (such as adenosine and thymidine), antibiotics (such as GENTAMYCIN™ drug), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range), and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to those skilled in the art. The culture conditions, such as temperature, pH, and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan.Purification of an Immunoglobulin-derived Structure of the Invention

[0341] Forms of immunoconjugate of the invention may be recovered from culture medium or from host cell lysates. If membrane-bound, it can be released from the membrane using a suitable detergent solution (e.g., Triton-X 100) or by enzymatic cleavage. Cells employed in expression of immunoconjugate of the invention can be disrupted by various physical or chemical means, such as freeze-thaw cycling, sonication, mechanical disruption, or cell lysing agents.

[0342] It may be desired to purify immunoconjugate of the invention from recombinant cell proteins or polypeptides. The following procedures are exemplary of suitable purification procedures: by fractionation on an ion-exchange column; ethanol precipitation; reverse phase HPLC; chromatography on silica or on a cation-exchange resin such as DEAE; chromatofocusing; SDS-PAGE; ammonium sulfate precipitation; gel filtration using, for example, Sephadex G-75; protein A Sepharose columns to remove contaminants such as IgG; and metal chelating columns to bind epitope-tagged forms of the immunoconjugate of the invention. Various methods of protein purification may be employed and such methods are known in the art and described for example in Deutscher, Methods in Enzymology, 182 (1990); Scopes, Protein Purification: Principles and Practice, Springer-Verlag, New York (1982). The purification step(s) selected will depend, for example, on the nature of the production process used and the particular immunoconjugate of the invention produced.

[0343] When using recombinant techniques, the immunoconjugate can be produced intracellularly, in the periplasmic space, or directly secreted into the medium. If the immunoconjugate is produced intracellularly, as a first step, the particulate debris, either host cells or lysed fragments, are removed, for example, by centrifugation or ultrafiltration. Carter et al., Bio / Technology 10: 163-7 (1992) describe a procedure for isolating antibodies which are secreted to the periplasmic space of E. coli. Briefly, cell paste is thawed in the presence of sodium acetate (pH 3.5), EDTA, and phenylmethylsulfonylfluoride (PMSF) over about 30 min. Cell debris can be removed by centrifugation. Where the immunoconjugate is secreted into the medium, supernatants from such expression systems are generally first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit. A protease inhibitor such as PMSF may be included in any of the foregoing steps to inhibit proteolysis and antibiotics may be included to prevent the growth of adventitious contaminants.

[0344] The immunoconjugate composition prepared from the cells can be purified using, for example, hydroxylapatite chromatography, gel electrophoresis, dialysis, and affinity chromatography, with affinity chromatography being a preferred purification technique. The suitability of protein A as an affinity ligand depends on the species and isotype of any immunoglobulin Fc domain that is present in the immunoconjugate. Protein A can be used to purify antibodies that are based on human yl, y2 or y4 heavy chains (Lindmark et al., J. Immunol. Meth. 62: 1-13 (1983)). Protein G is recommended for all mouse isotypes and for human y3 (Guss et al., EMBO J. 5: 15671575 (1986)). The matrix to which the affinity ligand is attached is most often agarose, but other matrices are available. Mechanically stable matrices such as controlled pore glass or poly(styrenedivinyl)benzene allow for faster flow rates and shorter processing times than can be achieved with agarose. Where the immunoconjugate comprises a CH3 domain, the Bakerbond ABX™ resin (J. T. Baker, Phillipsburg, NJ) is useful for purification. Other techniques for protein purification such as fractionation on an ion-exchange column, ethanol precipitation, Reverse Phase HPLC, chromatography on silica, chromatography on heparin SEPHAROSE™ chromatography on an anion or cation exchange resin (such as a polyaspartic acid column), chromatofocusing, SDS-PAGE, and ammonium sulfate precipitation are also available depending on the immunoconjugate to be recovered.

[0345] Following any preliminary purification step(s), the mixture comprising the immunoconjugate of interest and contaminants may be subjected to low pH hydrophobic interaction chromatography using an elution buffer at a pH between about 2.5-4.5, and generally at low salt concentrations (e.g., from about 0-0.25M salt).Immunoconjugates (including Antibody Drug Conjugates (ADCs))

[0346] In a further aspect of the invention, an immunoconjugate of the invention according to any of the above embodiments or described herein is conjugated to a heterologous moiety or agent, such as, e.g., as described below and including any additional exogenous material as described herein.

[0347] In one embodiment, the invention provides immunoconjugates comprising an antibody construct of the present invention conjugated to one or more therapeutic agents or radioactive isotopes.

[0348] In some embodiments, an immunoconjugate comprises an antibody construct as described herein conjugated to a radioactive atom to form a radioconjugate. As described herein, a variety of radioactive isotopes are available for the production of radioconjugates of the invention.

[0349] Conjugates of an immunoconjugate or antibody construct may be made using a variety of bifunctional protein coupling agents such as N-succinimidyl-3 -(2 -pyridyldithio) propionate (SPDP), succinimidyl-4-(N-maleimidomethyl) cyclohexane-l-carboxylate (SMCC), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate H ), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis (p-azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis- (pdiazoniumbenzoyl)-ethylenediamine), diisocyanates (such as toluene 2,6-diisocyanate), and bis-active fluorine compounds (such as l,5-difluoro-2,4-dinitrobenzene). For example, a ricin immunotoxin can be prepared as described in Vitetta et al., Science 238: 1098 (1987). Carbon- 14- labeled l-isothiocyanatobenzyl-3-methyldiethylene triaminepentaacetic acid (MX-DTPA) is an illustrative chelating agent for conjugation of radionucleotide to the antibody (see e.g., WO 1994 / 11026). The linker may be a “cleavable linker” facilitating release of a cytotoxic drug in the cell. For example, an acid-labile linker, peptidase-sensitive linker, photolabile linker, dimethyl linker or disulfide-containing linker may be used (see e.g., Chari et al., Cancer Res. 52: 127-131 (1992); US 5,208,020).

[0350] The immunoconjugates or ADCs herein expressly contemplate, but are not limited to such conjugates prepared with cross-linker reagents including, but not limited to, BMPS, EMCS, GMBS, HBVS, LC-SMCC, MBS, MPBH, SBAP, SIA, SIAB, SMCC, SMPB, SMPH, sulfo- EMCS, sulfo-GMBS, sulfo-KMUS, sulfo-MBS, sulfo-SIAB, sulfo-SMCC, and sulfo-SMPB, and SVSB (succinimidyl-(4-vinylsulfone)benzoate) which are commercially available (e.g., obtainable from Pierce Biotechnology, Inc., Rockford, IL., U.S.).

[0351] As recognized by the person of ordinary skill in the art, certain methods above are also useful to the preparation of radioimmunoconjugates and targeted imaging complexes (notwithstanding the textual reference to only immunoconjugates or antibody constructs), and such preparative methods are also embraced by the invention.Immunoconj ugation using Chelators and / or Linkers

[0352] Methods for affixing a radioisotope to an immunoconjugate or antibody construct (i.e., “labeling” an antibody with a radioisotope) are well known to the skilled worker. Certain of these methods are described, for example, in WO 2017 / 155937.

[0353] Bifunctional chelators, such as, e.g., DOTA, DTP A, and related analogs are suitable for coordinating metal ions like a and P-emitting radionuclides. For example, these chelating molecules can be linked to the targeting molecule by forming a new amide bond between an amine on the antibody construct (e.g., a functional group of a lysine residue) and a carboxylate on the DOTA / DTPA. In the case of peptide synthesis, characterization and purification of the linker addition can be part of the overall synthesis of an antibody platform or immunoconjugate for radioisotope conjugation.

[0354] For some embodiment, the method of producing an immunoconjugate involves a click chemistry step described by Poty, S et al., Chem Commun. (Camb) 54: 2599 (2018).

[0355] For some embodiments, a peptide may be biosynthesized or may be synthesized by chemical amino acid synthesis using suitable amino acid precursors involving, for example, fluorine-19 in place of hydrogen. In some embodiments, radiolabels may be incorporated into peptide. In some embodiments, radiolabels may be linked to peptide. The IODOGEN method (Fraker et al. (1978) Biochem Biophys Res Commun. 80: 49-57 can be used to incorporate iodine- 123. “Monoclonal Antibodies in Immunoscintigraphy” (Chatal, CRC Press 1989) describes other methods in detail.Characterization of Immunoconjugates of the Present Invention

[0356] Immunoconjugates of the present invention may be identified, screened for, or characterized for their phy si cal / chemi cal properties and / or biological activities by various assays known in the art. The immunoconjugates and antibody constructs of the invention may be characterized for their phy si cal / chemi cal properties and / or biological activities by various assays known in the art. Immunoconjugates of the invention can be characterized by a series of assays including, but not limited to, polypeptide sequence determination, amino acid analysis, nondenaturing size exclusion high pressure liquid chromatography (HPLC), mass spectrometry, ion exchange chromatography, and papain digestion.Antigen Binding

[0357] An immunoconjugate of the present invention may be tested for its antigen binding activity by methods known in the art, e.g., ELISA, Western blot, etc. The binding affinity of an antibody can, for example, be determined by the Scatchard analysis described in Munson et al., Anal Biochem. 107: 220 (1980). Further, the antigen binding ability of an immunoconjugate of the invention may be quantitated using methods known in the art, e.g., a quantitative ELISA, quantitative Western blot, surface plasmon resonance assay, and / or a Scatchard analysis.

[0358] In one embodiment, the KD of an immunoconjugate is measured using a radiolabeled antigen ELISA performed with the immunoconjugate. According to another embodiment, the KD is measured by using surface-plasmon resonance assays using a BIACORE®-2000 or a BIACORE®-3000 instrument (BIAcore, Inc., Piscataway, N.J.), e.g., using immobilized antigen CM5 chips at 25° C and 10 response units.

[0359] In another aspect, binding competition assays may be used to identify immunoconjugates that compete for binding to the same antigen, or epitope thereof. In some embodiments, such a competing antibody binds to the same epitope (e.g., a linear or a conformational epitope) of an immunoconjugate of the invention (see e.g., Harlow and Lane (1988) Antibodies: A Laboratory Manual, Ch.14 (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)).

[0360] The epitope and / or contact residues within an antigen bound by an immunoconjugate of the invention can be identified or mapped using methods known to the skilled worker. Detailed exemplary methods for mapping an epitope to which an antibody binds are provided in Morris (1996) “Epitope Mapping Protocols,” in Methods in Molecular Biology (3rded., Humana Press, Totowa, NJ).Pharmaceutical Compositions and Formulations of the Present Invention

[0361] As will be recognized by the person of ordinary skill in the art, certain teachings herein below apply to immunoconjugates and radioimmunoconjugates of the invention, notwithstanding the specific textual reference to one type of invention, and such applications are embraced in entirety by the invention.

[0362] In another aspect, the invention provides a composition comprising an immunoconjugate or radioimmunoconjugate of the present invention. The invention further provides pharmaceutical compositions and formulations comprising at least one immunoconjugate of the present invention and at least one pharmaceutically acceptable excipient or carrier. In some embodiments, a pharmaceutical formulation comprises (1) animmunoconjugate or radioimmunoconjugate of the invention, and (2) a pharmaceutically acceptable carrier.

[0363] An immunoconjugate or radioimmunoconjugate is formulated in any suitable form for delivery to a target cell / tissue. Pharmaceutical formulations of an immunoconjugate of the present invention are prepared by mixing such immunoconjugate having the desired degree of purity with one or more optional pharmaceutically acceptable carriers, diluents, and / or excipients (Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980)), in the form of lyophilized formulations or aqueous solutions. Pharmaceutically acceptable carriers, diluents, and excipients are generally nontoxic to recipients at the dosages and concentrations employed, and include, but are not limited to: sterile water, buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride; benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3 -pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; metal complexes (e.g., Zn-protein complexes); and / or non-ionic surfactants such as polyethylene glycol (PEG).

[0364] Pharmaceutical formulations to be used for in vivo administration are generally sterile. This is readily accomplished by filtration through sterile filtration membranes.

[0365] Examples of lyophilized antibody formulations are described in US 6,267,958. Aqueous antibody formulations include those described in US 6,171,586 and WO 2006 / 044908, the latter formulations including a histidine-acetate buffer.

[0366] Pharmaceutically acceptable carriers herein further include interstitial drug dispersion agents such as soluble neutral -active hyaluronidase glycoproteins (sHASEGP), for example, human soluble PH-20 hyaluronidase glycoproteins, such as rHuPH20 (HYLENEX ®, Baxter International, Inc.). In one aspect, a sHASEGP is combined with one or more additional glycosaminoglycanases such as chondroitinases.

[0367] The formulation herein may also contain more than one active ingredient as necessary for the particular indication being treated, preferably those with complementary activities that donot adversely affect each other. Such active ingredients are suitably present in combination in amounts that are effective for the purpose intended.

[0368] The active ingredients may also be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nanoparticles and nanocapsules) or in macroemulsions. Such techniques are disclosed in Remington ’s Pharmaceutical Sciences, 16th edition, Osol, A. Ed. (1980).

[0369] In some embodiments, immunoconjugates may be formulated as immunoliposomes. A “liposome” is a small vesicle composed of various types of lipids, phospholipids and / or surfactant which is useful for delivery of a drug to a mammal. The components of the liposome are commonly arranged in a bilayer formation, similar to the lipid arrangement of biological membranes. Liposomes containing the immunoconjugate are prepared by methods known in the art, such as described in Epstein et al., Proc Natl Acad Sci USA 82: 3688 (1985); Hwang et al., Proc Natl Acad Sci USA 77: 4030 (1980); U.S. Pat. Nos. 4,485,045 and 4,544,545; and WO1997 / 38731 published October 23, 1997. Particularly useful liposomes can be generated by the reverse phase evaporation method with a lipid composition comprising phosphatidylcholine, cholesterol and PEG-derivatized phosphatidylethanolamine (PEG-PE). Liposomes are extruded through filters of defined pore size to yield liposomes with the desired diameter. A chemotherapeutic agent is optionally contained within the liposome (see Gabizon et al., J. National Cancer Inst. 81 : 1484 (1989)). Liposomes with enhanced circulation time are disclosed in U.S. Patent No. 5,013,556.

[0370] Sustained-release preparations may be prepared. Suitable examples of sustained- release preparations include semipermeable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g., films, or microcapsules.Methods of Using Immunoconjugates and Radioimmunoconjugates and Compositions Thereof

[0371] In one aspect, the invention provides a method of treating a disease, disorder, or condition in a patient in need thereof, the method comprising administering to a subject in need thereof a pharmaceutically effective amount of an immunoconjugate or radioimmunoconjugate or composition of the present invention. For some further embodiments, the method is for inhibiting the growth and / or the killing of a cancer cell or tumor. In another aspect, the invention provides for the use of an immunoconjugate described herein for the preparation and / or manufacture of a medicament for treating a disease, disorder, or condition in a subject, such as, e.g., cancer.

[0372] Pharmaceutical compositions of the present invention may be administered in a manner appropriate to the disease to be treated (or prevented). The quantity and frequency of administration will be determined by such factors as the condition of the patient, and the type and severity of the patient’s disease, although appropriate dosages may be determined by clinical trials.

[0373] In one embodiment, an immunoconjugate or radioimmunoconjugate or composition of the invention can be used in a method for binding target antigen in an individual suffering from a disorder associated with increased target antigen expression and / or activity, the method comprising administering to the individual the immunoconjugate or radioimmunoconjugate or composition such that target antigen in the individual is bound. In one embodiment, the target antigen is human target antigen, and the individual is a human individual. An immunoconjugate or radioimmunoconjugate or composition of the invention can be administered to a human for therapeutic purposes. Moreover, an immunoconjugate or radioimmunoconjugate or composition of the invention can be administered to a non-human mammal expressing target antigen with which the immunoconjugate or radioimmunoconjugate cross-reacts (e.g., a primate, pig, rat, or mouse) for veterinary purposes or as an animal model of human disease. Regarding the latter, such animal models may be useful for evaluating the therapeutic efficacy of an immunoconjugate or radioimmunoconjugate or composition of the invention (e.g., testing of dosages and time courses of administration).

[0374] An immunoconjugate or radioimmunoconjugate or composition of the invention (and any additional therapeutic agent or adjuvant) can be administered by any suitable means, including parenteral, subcutaneous, intraperitoneal, intrapulmonary, and intranasal, and, if desired for local treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration. In addition, the antibody is suitably administered by pulse infusion, particularly with declining doses of the antibody. Dosing can be by any suitable route, e.g., by injections, such as intravenous or subcutaneous injections, depending in part on whether the administration is brief or chronic.

[0375] Immunoconjugate or radioimmunoconjugate or compositions of the invention would be formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. The immunoconjugates of the invention are administered to a human patient, in accordance with known methods, such as intravenousadministration, e.g., as a bolus or by continuous infusion over a period of time, by intramuscular, intraperitoneal, intracerobrospinal, subcutaneous, intra-articular, intrasynovial, intrathecal, oral, topical, or inhalation routes. For some embodiments, intravenous or subcutaneous administration of the immunoconjugate or radioimmunoconjugate or composition of the invention is preferred.

[0376] For the prevention or treatment of disease, the dosage and mode of administration will be chosen by the physician according to known criteria. The appropriate dosage of immunoconjugate or radioimmunoconjugate or composition of the invention will depend on the type of disease to be treated, as defined above, the severity and course of the disease, whether the immunoconjugate or radioimmunoconjugate or composition of the invention is administered for preventive or therapeutic purposes, previous therapy, the patient’s clinical history and response to the immunoconjugate or radioimmunoconjugate or composition, and the discretion of the attending physician. The immunoconjugate or radioimmunoconjugate or composition of the invention is suitably administered to the patient at one time or over a series of treatments. Preferably, the immunoconjugate or radioimmunoconjugate or composition is administered by intravenous infusion or by subcutaneous injections. Depending on the type and severity of the disease, about 1 pg / kg to about 50 mg / kg body weight (e.g., about 0.1-15 mg / kg / dose) of immunoconjugate or radioimmunoconjugate or composition can be an initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion. A dosing regimen can comprise administering an initial loading dose of about 4 mg / kg, followed by a weekly maintenance dose of about 2 mg / kg of the immunoconjugate or radioimmunoconjugate or composition of the invention. However, other dosage regimens may be useful. A typical daily dosage might range from about 1 pg / kg to 100 mg / kg or more, depending on the factors mentioned above. For repeated administrations over several days or longer, depending on the condition, the treatment is sustained until a desired suppression of disease symptoms occurs. The progress of this therapy can be readily monitored by conventional methods and assays and based on criteria known to the physician or other persons of skill in the art.

[0377] The dose and administration schedule may be selected and adjusted based on the level of disease, or tolerability in the subject, which may be monitored during the course of treatment. The conjugates of the present invention may administered once per day, once per week, multiple times per week, but less than once per day, multiple times per month but less than once per day, multiple times per month but less than once per week, once per month, once per five weeks, once per six weeks, once per seven weeks, once per eight weeks, once per nine weeks, once per ten weeks, or intermittently to relieve or alleviate symptoms of the disease. Administration may continue at any of the disclosed intervals until remission of the tumor or symptoms of the cancerbeing treated. Administration may continue after remission or relief of symptoms is achieved where such remission or relief is prolonged by such continued administration.

[0378] For some embodiments, the effective amount of the immunoconjugate or radioimmunoconjugate or composition may be provided as a single dose.

[0379] The Immunoconjugates and radioimmunoconjugates of the present invention maybe used in combination with conventional and / or novel methods of treatment or therapy or separately as a monotherapy. In some embodiments, the immunoconjugates and radioimmunoconjugates of the present invention maybe used with one or more radiation sensitizer agents. Such agents include any agent that can increase the sensitivity of cancer cells to radiation therapy. In other embodiments, immunoconjugates and radioimmunoconjugates of the present invention may be used in combination with novel and / or conventional agents that can augment the biological effects of radiotherapy. Irradiation of a tumor can cause a variety of biological consequences which can be exploited by combining immunoconjugates and radioimmunoconjugates of the present invention with agents that target relevant pathways. In some embodiments, such agents may reduce tumor angiogenesis, or inhibit local invasion and metastasis, or prevent repopulation, or augment the immune response, or deregulate cellular energetics, or reduce population, or alter tumor metabolism, or increase tumor damage, or reduce DNA repair. In certain embodiments, agents for use in combination with immunoconjugates and radioimmunoconjugates of the present invention may comprise DDR inhibitors, e.g., PARP, ATR, Chkl, or DNA-PK; or survival signaling inhibitors, e.g., mTOR, PI3k, NF-kB; or antihypoxia agents, e.g, HIF-1 -alpha, CAP, or UPR; or metabolic inhibitors, e.g., MCT1, MCT4 inhibitors; or immunotherapeutics, e.g., anti- CTLA4, anti-PD-1; or inhibitors of growth factor signal transduction, e.g., EGFR or MAPK inhibitors; or anti-invasives, e.g., kinase inhibitors, chemokine inhibitors, or integrin inhibitors; or anti-angiogenic agents, e.g., VEGF -inhibitors.

[0380] Immunoconjugates and radioimmunoconjugates of the present invention may (i) inhibit the growth or proliferation of a cell to which they bind; (ii) induce the death of a cell to which they bind; (iii) inhibit the delamination of a cell to which they bind; (iv) inhibit the metastasis of a cell to which they bind; or (v) inhibit the vascularization of a tumor comprising a cell to which they bind. In this context, “inhibiting cell growth or proliferation” means decreasing a cell’s growth or proliferation by at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 100%, and includes inducing cell death.

[0381] By way of example, an immunoconjugate that inhibits the growth of a tumor cell is one that results in measurable growth inhibition of a tumor cell (e.g., a cancer cell). In one embodiment, an immunoconjugate or radioimmunoconjugate of the invention is capable ofinhibiting the growth of cancer cells displaying the antigen bound by the immunoconjugate or radioimmunoconjugate. Preferred growth inhibitory immunoconjugates or radioimmunoconjugates inhibit growth of antigen-expressing tumor cells by greater than 20%, preferably from about 20% to about 50%, and even more preferably, by greater than 50% (e.g., from about 50% to about 100%) as compared to the appropriate control, the control typically being tumor cells not treated with the immunoconjugate or radioimmunoconjugate being tested.

[0382] For some embodiments, a majority of the immunoconjugate or radioimmunoconjugate or composition administered to a subject typically consists of non-labeled immunoconjugate, with the minority being labeled radioimmunoconjugate. The ratio of labeled radioimmunoconjugate to non-labeled immunoconjugate can be adjusted using known methods. Thus, accordingly to certain aspects of the present invention, the immunoconjugate / radioimmunoconjugate may be provided in a total protein amount of up to 100 mg, such as less than 60 mg, or from 5 mg to 45 mg, or a total protein amount of between 0.1 pg / kg to 1 mg / kg patient weight, such as 1 pg / kg to 1 mg / kg patient weight, or 10 pg / kg to 1 mg / kg patient weight, or 100 pg / kg to 1 mg / kg patient weight, or 0.1 pg / kg to 100 pg / kg patient weight, or 0.1 pg / kg to 50 pg / kg patient weight, or 0.1 pg / kg to 10 pg / kg patient weight, or 0.1 pg / kg to 40 pg / kg patient weight, or 1 pg / kg to 40 pg / kg patient weight, or 0.1 mg / kg to 1.0 mg / kg patient weight, such as from 0.2 mg / kg patient weight to 0.6 mg / kg patient weight.

[0383] In certain embodiments, the immunoconjugate / radioimmunoconjugate may be administered from about 0.5 mg / kg to about 30 mg / kg. In certain embodiments, the immunoconjugate / radioimmunoconjugate may be administered from about 0.5 mg / kg to about 1 mg / kg, about 0.5 mg / kg to about 2 mg / kg, about 0.5 mg / kg to about 5 mg / kg, about 0.5 mg / kg to about 10 mg / kg, about 0.5 mg / kg to about 3 mg / kg, about 0.5 mg / kg to about 4 mg / kg, about 0.5 mg / kg to about 5 mg / kg, about 0.5 mg / kg to about 10 mg / kg, about 0.5 mg / kg to about 20 mg / kg, about 0.5 mg / kg to about 30 mg / kg, about 1 mg / kg to about 2 mg / kg, about 1 mg / kg to about 5 mg / kg, about 1 mg / kg to about 10 mg / kg, about 1 mg / kg to about 3 mg / kg, about 1 mg / kg to about 4 mg / kg, about 1 mg / kg to about 5 mg / kg, about 1 mg / kg to about 10 mg / kg, about 1 mg / kg to about 20 mg / kg, about 1 mg / kg to about 30 mg / kg, about 2 mg / kg to about 5 mg / kg, about 2 mg / kg to about 10 mg / kg, about 2 mg / kg to about 3 mg / kg, about 2 mg / kg to about 4 mg / kg, about 2 mg / kg to about 5 mg / kg, about 2 mg / kg to about 10 mg / kg, about 2 mg / kg to about 20 mg / kg, about 2 mg / kg to about 30 mg / kg, about 5 mg / kg to about 10 mg / kg, about 5 mg / kg to about 3 mg / kg, about 5 mg / kg to about 4 mg / kg, about 5 mg / kg to about 5 mg / kg, about 5 mg / kg to about 10 mg / kg, about 5 mg / kg to about 20 mg / kg, about 5 mg / kg to about 30 mg / kg, about 10 mg / kg to about 3 mg / kg, about 10 mg / kg to about 4 mg / kg, about 10 mg / kg to about 5 mg / kg, about 10mg / kg to about 10 mg / kg, about 10 mg / kg to about 20 mg / kg, about 10 mg / kg to about 30 mg / kg, about 3 mg / kg to about 4 mg / kg, about 3 mg / kg to about 5 mg / kg, about 3 mg / kg to about 10 mg / kg, about 3 mg / kg to about 20 mg / kg, about 3 mg / kg to about 30 mg / kg, about 4 mg / kg to about 5 mg / kg, about 4 mg / kg to about 10 mg / kg, about 4 mg / kg to about 20 mg / kg, about 4 mg / kg to about 30 mg / kg, about 5 mg / kg to about 10 mg / kg, about 5 mg / kg to about 20 mg / kg, about 5 mg / kg to about 30 mg / kg, about 10 mg / kg to about 20 mg / kg, about 10 mg / kg to about 30 mg / kg, or about 20 mg / kg to about 30 mg / kg. In certain embodiments, the immunoconjugate / radioimmunoconjugate may be administered at about 0.5 mg / kg, about 1 mg / kg, about 2 mg / kg, about 5 mg / kg, about 10 mg / kg, about 3 mg / kg, about 4 mg / kg, about 5 mg / kg, about 10 mg / kg, about 20 mg / kg, or about 30 mg / kg. In certain embodiments, the immunoconjugate / radioimmunoconjugate may be administered at least about 0.5 mg / kg, about 1 mg / kg, about 2 mg / kg, about 5 mg / kg, about 10 mg / kg, about 3 mg / kg, about 4 mg / kg, about 5 mg / kg, about 10 mg / kg, or about 20 mg / kg. In certain embodiments, the immunoconjugate / radioimmunoconjugate may be administered at most about 1 mg / kg, about 2 mg / kg, about 5 mg / kg, about 10 mg / kg, about 3 mg / kg, about 4 mg / kg, about 5 mg / kg, about 10 mg / kg, about 20 mg / kg, or about 30 mg / kg.

[0384] In some embodiments, the method comprises administering the effective amount of a radioimmunoconjugate comprising225Ac that is from 0.01 to 0.1 mCi, or 0.1 mCi to 1.0 mCi, or from 1.0 mCi to 2.0 mCi, or from 2.0 mCi to 4.0 mCi.

[0385] In some embodiments, the method comprises administering the effective amount of a radioimmunoconjugate comprising225Ac that is from 0.1 pCi / kg to 2.0 pCi / kg subject weight, or from 0.1 pCi / kg to 1.0 pCi / kg subject weight, or from 1.0 pCi / kg to 3.0 pCi / kg subject weight, or from 3.0 pCi / kg to 10.0 pCi / kg subject weight, or from 10.0 pCi / kg to 20.0 pCi / kg subject weight, or from 10.0 pCi / kg to 30.0 pCi / kg subject weight.

[0386] In certain embodiments, the effective amount of225Ac is about 0.1 microcurie to about 20 microcurie. In certain embodiments, the effective amount of225Ac is about 0.1 microcurie to about 0.2 microcurie, about 0.1 microcurie to about 0.5 microcurie, about 0.1 microcurie to about 1 microcurie, about 0.1 microcurie to about 2 microcurie, about 0.1 microcurie to about 3 microcurie, about 0.1 microcurie to about 4 microcurie, about 0.1 microcurie to about 5 microcurie, about 0.1 microcurie to about 10 microcurie, about 0.1 microcurie to about 20 microcurie, about 0.2 microcurie to about 0.5 microcurie, about 0.2 microcurie to about 1 microcurie, about 0.2 microcurie to about 2 microcurie, about 0.2 microcurie to about 3 microcurie, about 0.2 microcurie to about 4 microcurie, about 0.2 microcurie to about 5 microcurie, about 0.2 microcurie to about 10 microcurie, about 0.2 microcurie to about 20microcurie, about 0.5 microcurie to about 1 microcurie, about 0.5 microcurie to about 2 microcurie, about 0.5 microcurie to about 3 microcurie, about 0.5 microcurie to about 4 microcurie, about 0.5 microcurie to about 5 microcurie, about 0.5 microcurie to about 10 microcurie, about 0.5 microcurie to about 20 microcurie, about 1 microcurie to about 2 microcurie, about 1 microcurie to about 3 microcurie, about 1 microcurie to about 4 microcurie, about 1 microcurie to about 5 microcurie, about 1 microcurie to about 10 microcurie, about 1 microcurie to about 20 microcurie, about 2 microcurie to about 3 microcurie, about 2 microcurie to about 4 microcurie, about 2 microcurie to about 5 microcurie, about 2 microcurie to about 10 microcurie, about 2 microcurie to about 20 microcurie, about 3 microcurie to about 4 microcurie, about 3 microcurie to about 5 microcurie, about 3 microcurie to about 10 microcurie, about 3 microcurie to about 20 microcurie, about 4 microcurie to about 5 microcurie, about 4 microcurie to about 10 microcurie, about 4 microcurie to about 20 microcurie, about 5 microcurie to about 10 microcurie, about 5 microcurie to about 20 microcurie, or about 10 microcurie to about 20 microcurie. In certain embodiments, the effective amount of225Ac is about 0.1 microcurie, about 0.2 microcurie, about 0.5 microcurie, about 1 microcurie, about 2 microcurie, about 3 microcurie, about 4 microcurie, about 5 microcurie, about 10 microcurie, or about 20 microcurie. In certain embodiments, the effective amount of225Ac is at least about 0.1 microcurie, about 0.2 microcurie, about 0.5 microcurie, about 1 microcurie, about 2 microcurie, about 3 microcurie, about 4 microcurie, about 5 microcurie, or about 10 microcurie. In certain embodiments, the effective amount of225Ac is at most about 0.2 microcurie, about 0.5 microcurie, about 1 microcurie, about 2 microcurie, about 3 microcurie, about 4 microcurie, about 5 microcurie, about 10 microcurie, or about 20 microcurie. According to aspects where the radioisotope of the radioimmunoconjugate isU 1ln, the effective amount is below, for example, 15.0 mCi (i.e., where the amount ofi nIn administered to the subject delivers a total body radiation dose of below 15.0 mCi).

[0387] According to aspects where the radioisotope of the radioimmunoconjugate isU 1ln, the effective amount is below 15.0 mCi, below 14.0 mCi, below 13.0 mCi, below 12.0 mCi, below 11.0 mCi, below 10.0 mCi., below 9.0 mCi, below 8.0 mCi, below 7.0 mCi, below 6.0 mCi, below 5.0 mCi, below 4.0 mCi, below 3.5 mCi, below 3.0 mCi, below 2.5 mCi, below 2.0 mCi, below 1.5 mCi, below 1.0 mCi, below 0.5 mCi, below 0.4 mCi, below 0.3 mCi, below 0.2 mCi, or below 0.1 mCi.

[0388] According to aspects where the radioisotope of the radioimmunoconjugate isU 1ln, the effective amount is from 0.1 mCi to 1.0 mCi, from 0.1 mCi to 2.0 mCi, from 1.0 mCi to 2.0 mCi, from 1.0 mCi to 3.0 mCi, from 1.0 mCi to 4.0 mCi, from 1.0 mCi to 5.0 mCi, from 1.0 mCi to 10.0 mCi, from 1.0 mCi to 15.0 mCi, from 1.0 mCi to 20.0 mCi, from 2.0 mCi to 3.0 mCi, from3.0 mCi to 4.0 mCi, from 4.0 mCi to 5.0 mCi, from 5.0 mCi to 10.0 mCi, from 5.0 mCi to 15.0 mCi, from 5.0 mCi to 20.0 mCi, from 6.0 mCi to 14.0 mCi, from 7.0 mCi to 13.0 mCi, from 8.0 mCi to 12.0 mCi, from 9.0 mCi to 11.0 mCi, or from 10.0 mCi to 15.0 mCi.

[0389] According to aspects where the radioisotope of the radioimmunoconjugate isH 1In, the effective amount is 15.0 mCi, 14.0 mCi, 13.0 mCi, 12.0 mCi, 11.0 mCi, 10.0 mCi, 9.0 mCi, 8.0 mCi, 7.0 mCi, 6.0 mCi, 5.0 mCi, 4.0 mCi, 3.5 mCi, 3.0 mCi, 2.5 mCi, 2.0 mCi, 1.5 mCi, 1.0 mCi, 0.5 mCi, 0.4 mCi, 0.3 mCi, 0.2 mCi, or 0.1 mCi.

[0390] According to aspects where the radioisotope of the radioimmunoconjugate is225Ac, the effective amount is below, for example, 30.0 pCi / kg (i.e., where the amount of225Ac administered to the subject delivers a radiation dose of below 30.0 pCi per kilogram of subject’s body weight).

[0391] According to aspects where the radioisotope of the radioimmunoconjugate is225Ac, the effective amount is below 30 pCi / kg, 25 pCi / kg, 20 pCi / kg, 17.5 pCi / kg, 15.0 pCi / kg, 12.5 pCi / kg, 10.0 pCi / kg, 9 pCi / kg, 8 pCi / kg, 7 pCi / kg, 6 pCi / kg, 5 pCi / kg, 4.5 pCi / kg, 4.0 pCi / kg, 3.5 pCi / kg, 3.0 pCi / kg, 2.5 pCi / kg, 2.0 pCi / kg, 1.5 pCi / kg, 1.0 pCi / kg, 0.9 pCi / kg, 0.8 pCi / kg, 0.7 pCi / kg, 0.6 pCi / kg, 0.5 pCi / kg, 0.4 pCi / kg, 0.3 pCi / kg, 0.2 pCi / kg, 0.1 pCi / kg, or 0.05 pCi / kg.

[0392] According to aspects where the radioisotope of the radioimmunoconjugate is225Ac, the effective amount is from 0.05 pCi / kg to 0 .1 pCi / kg, from 0 .1 pCi / kg to 0.2 pCi / kg, from 0.2 pCi / kg to 0.3 pCi / kg, from 0.3 pCi / kg to 0.4 pCi / kg, from 0.4 pCi / kg to 0.5 pCi / kg, from 0.5 pCi / kg to 0.6 pCi / kg, from 0.6 pCi / kg to 0.7 pCi / kg, from 0.7 pCi / kg to 0.8 pCi / kg, from 0.8 pCi / kg to 0.9 pCi / kg, from 0.9 pCi / kg to 1.0 pCi / kg, from 1.0 pCi / kg to 1.5 pCi / kg, from 1.5 pCi / kg to 2.0 pCi / kg, from 2.0 pCi / kg to 2.5 pCi / kg, from 2.5 pCi / kg to 3.0 pCi / kg, from 3.0 pCi / kg to 3.5 pCi / kg, from 3.5 pCi / kg to 4.0 pCi / kg, from 4.0 pCi / kg to 4.5 pCi / kg, or from 4.5 pCi / kg to 5.0 pCi / kg.

[0393] According to aspects where the radioisotope of the radioimmunoconjugate is225Ac, the effective amount is 0.05 pCi / kg, 0.1 pCi / kg, 0.2 pCi / kg, 0.3 pCi / kg, 0.4 pCi / kg, 0.5 pCi / kg, 0.6 pCi / kg, 0.7 pCi / kg, 0.8 pCi / kg, 0.9 pCi / kg, 1.0 pCi / kg, 1.5 pCi / kg, 2.0 pCi / kg, 2.5 pCi / kg, 3.0 pCi / kg, 3.5 pCi / kg, 4.0 pCi / kg or 4.5 pCi / kg, 5.0 pCi / kg, 6.0 pCi / kg, 7.0 pCi / kg, 8.0 pCi / kg, 9.0 pCi / kg, 10.0 pCi / kg, 12.5 pCi / kg, 15.0 pCi / kg, 17.5 pCi / kg, 20.0 pCi / kg, 25 pCi / kg, or 30 pCi / kg.

[0394] In certain embodiments where the radioisotope of the radioimmunoconjugate is177Lu the effective amount is from 0.1 pCi to 100 mCi per meter squared of body surface area.

[0395] In certain embodiments where the radioisotope of the radioimmunoconjugate is177Lu the effective amount is from 1 mCi to 100 mCi per meter squared of body surface area. In certainembodiments, the effective amount is about 1 per meter squared to about 100 per meter squared. In certain embodiments, the effective amount is about 1 per meter squared to about 5 per meter squared, about 1 per meter squared to about 10 per meter squared, about 1 per meter squared to about 15 per meter squared, about 1 per meter squared to about 20 per meter squared, about 1 per meter squared to about 25 per meter squared, about 1 per meter squared to about 75 per meter squared, about 1 per meter squared to about 100 per meter squared, about 5 per meter squared to about 10 per meter squared, about 5 per meter squared to about 15 per meter squared, about 5 per meter squared to about 20 per meter squared, about 5 per meter squared to about 25 per meter squared, about 5 per meter squared to about 75 per meter squared, about 5 per meter squared to about 100 per meter squared, about 10 per meter squared to about 15 per meter squared, about 10 per meter squared to about 20 per meter squared, about 10 per meter squared to about 25 per meter squared, about 10 per meter squared to about 75 per meter squared, about 10 per meter squared to about 100 per meter squared, about 15 per meter squared to about 20 per meter squared, about 15 per meter squared to about 25 per meter squared, about 15 per meter squared to about 75 per meter squared, about 15 per meter squared to about 100 per meter squared, about 20 per meter squared to about 25 per meter squared, about 20 per meter squared to about 75 per meter squared, about 20 per meter squared to about 100 per meter squared, about 25 per meter squared to about 75 per meter squared, about 25 per meter squared to about 100 per meter squared, or about 75 per meter squared to about 100 per meter squared. In certain embodiments, the effective amount is about 1 per meter squared, about 5 per meter squared, about 10 per meter squared, about 15 per meter squared, about 20 per meter squared, about 25 per meter squared, about 75 per meter squared, or about 100 per meter squared. In certain embodiments, the effective amount is at least about 1 per meter squared, about 5 per meter squared, about 10 per meter squared, about 15 per meter squared, about 20 per meter squared, about 25 per meter squared, or about 75 per meter squared. In certain embodiments, the effective amount is at most about 5 per meter squared, about 10 per meter squared, about 15 per meter squared, about 20 per meter squared, about 25 per meter squared, about 75 per meter squared, or about 100 per meter squared.

[0396] According to certain aspects of the present invention, a preparation of radioimmunoconjugate of the invention, or a composition thereof (e.g., a pharmaceutical composition), may comprise a radiolabeled fraction (radioimmunoconjugate) and an unlabeled fraction (immunoconjugate), wherein the ratio of lab eled: unlab eled may be from about 1 : 1000 to 1 : 1.

[0397] Moreover, the pharmaceutical compositions may be provided as a single dose composition tailored to a specific patient, i.e., as a patient specific therapeutic composition,wherein the amount of labeled and unlabeled immunoconjugate (labeled immunoconjugate, for clarity, being the same as radioimmunoconjugate herein) in the composition may depend on at least a patient weight, height, body surface area, age, gender, and / or disease state or health status. As such, a total volume of the patient specific therapeutic composition may be provided in a vial that is configured to be wholly administered to the patient in one treatment session, such that little to no composition remains in the vial after administration.

[0398] Currently, depending on the stage of the cancer, cancer treatment involves one or a combination of the following therapies: surgery to remove the cancerous tissue, radiation therapy, and chemotherapy. Therapy using radioimmunoconjugate of the invention (interchangeably, “radiolabeled immunoconjugate”) may be especially desirable in elderly patients who do not tolerate the toxicity and side effects of chemotherapy well and in metastatic disease where radiation therapy has limited usefulness. For some embodiments, therapy using radiolabeled immunoconjugate of the invention are useful to alleviate target antigen-expressing cancers upon initial diagnosis of the disease or during relapse.

[0399] In some embodiments, determining whether a cancer is amenable to treatment by methods disclosed herein involves detecting the presence of the target antigen in a subject or in a sample from a subject. To determine target antigen expression in a cancer, various detection assays are available. In one embodiment, target antigen overexpression is analyzed by immunohistochemistry (IHC). Parrafin embedded tissue sections from a tumor biopsy are subjected to the IHC assay and accorded a target antigen staining intensity criteria. Alternatively, or additionally, FISH assays such as the INFORM® (sold by Ventana, AZ, U.S.A.) or PATHVISION® (Vysis, IL, U.S.A.) may be carried out on formalin-fixed, paraffin-embedded tumor tissue to determine the extent (if any) of target antigen overexpression in the tumor.

[0400] Target antigen overexpression or amplification may be evaluated using an in vivo detection assay, e.g., by administering a molecule (such as an antibody construct or immunoconjugate of the invention) which binds the molecule to be detected and is tagged with a detectable label (e.g., a radioactive isotope or a fluorescent label) and externally scanning the patient for localization of the label.Using I mmu noconjugates and Radioimmunoconjugates of the Invention for Killing a Cell s)

[0401] An immunoconjugate or radioimmunoconjugate of the invention may be used in, for example, in vitro, ex vivo, and in vivo methods. In one aspect, the invention provides methods for inhibiting cell growth or proliferation, either in vivo or in vitro, the method comprising exposing a cell to an immunoconjugate or radioimmunoconjugate of the invention under conditions permissive for binding of the immunoconjugate or radioimmunoconjugate to a target antigen. Theimmunoconjugate or radioimmunoconjugate of the invention may also (i) inhibit the growth or proliferation of a cell to which they bind; (ii) induce the death of a cell to which they bind; (iii) inhibit the delamination of a cell to which they bind; (iv) inhibit the metastasis of a cell to which they bind; or (v) inhibit the vascularization of a tumor comprising a cell to which they bind.

[0402] In one aspect, the invention provides a method of killing an antigen expressing cell, the method comprising contacting the cell with an immunoconjugate or radioimmunoconjugate of the present invention (or a composition thereof). This method can be used, e.g., to kill, deplete, or eliminate target antigen-expressing cells from a population of mixed cells. This method can be used, e.g., to kill, deplete, or eliminate target antigen-expressing cells from a population of mixed cells as a step in the purification of other cells. This method can be performed in vitro or in vivo, including ex vivo on primary patient cell or tissue compositions to prepare such compositions for transplantation.

[0403] In one aspect, an immunoconjugate or radioimmunoconjugate of the invention is used to treat or prevent a cell proliferative disorder. In certain embodiments, the cell proliferative disorder comprises a solid tumor cancer. A solid tumor cancer is a cancer comprising an abnormal mass of tissue, e.g., carcinomas and sarcomas. In certain other embodiments, the cell proliferative disorder comprises a liquid tumor cancer or hematological cancer, Used interchangeably, such cancers present in the body fluid, e.g., leukemias and lymphomas. In certain embodiments, the cell proliferative disorder is associated with increased expression and / or activity of a target antigen. For example, in certain embodiments, the cell proliferative disorder is associated with increased expression of target antigen on the surface of a cell. In certain embodiments, the cell proliferative disorder is a tumor or a cancer. In certain embodiments, the cell proliferative disorder comprises a solid tumor cancer. A solid tumor cancer is a cancer comprising an abnormal mass of tissue, e.g., carcinomas and sarcomas. In certain other embodiments, the cell proliferative disorder comprises a liquid tumor cancer or hematological cancer, Used interchangeably, such cancers present in the body fluid, e.g., leukemias and lymphomas.

[0404] In one aspect, the invention provides methods for treating a cell proliferative disorder comprising administering to an individual an effective amount of an immunoconjugate or radioimmunoconjugate of the invention.

[0405] In addition to direct cell killing of target cells expressing cell-surface antigen specifically bound by the immunoconjugate or radioimmunoconjugate of the invention, the immunoconjugate or radioimmunoconjugate of the present invention optionally may be used for delivery of additional cargos to the vicinity of or the interiors of target cells. The delivery of additional exogenous materials may be used, e.g., for cytotoxic, cytostatic, information gathering,and / or diagnostic functions. Non-cytotoxic variants of the immunoconjugate or radioimmunoconjugate of the invention, or optionally toxic variants, may be used to deliver cargos to and / or label the interiors of cells expressing the target antigen. Non-limiting examples of cargos include cytotoxic agents, detection-promoting agents, and small molecule chemotherapeutic agents.Using Antibody Constructs, Immunoconi ugates Radioimmunoconjugates and Targeted Imaging Complexes of the Invention for Antigen Detection, In Vivo Imaging, Diagnosis, and Prognostication

[0406] As described herein, in some embodiments, the antibody constructs, immunoconjugates, radioimmunoconjugates and targeted imaging complexes of the present invention have various non-therapeutic applications. In some embodiments, the compositions of the invention may be used to identify patient populations predicted to benefit from a specific therapeutic approach or modality, such as, e.g., treatment with an immunoconjugates or radioimmunoconjugates of the invention. In some embodiments, the compositions of the invention can be useful for staging of target antigen expressing cancers (e.g., by radioimaging) or as prognostic indicators of disease progression. In some embodiments, the compositions are also useful for detection and quantitation of a target epitope in vitro, e.g., in an ELISA or a Western blot, as well as purification or immunoprecipitation of a target antigen from cells or a tissue sample.

[0407] For some embodiments, the immunoconjugate or radioimmunoconjugate of the invention is used in a method to detect the presence of or level of an antigen, such as, e.g., in vitro in a biological sample or in vivo using an imagine technique. Immunoconjugate and radioimmunoconjugate detection can be achieved via different techniques known to the skilled worker and as described herein, e.g., IHC and PET imaging. When an immunoconjugate or radiolabeled immunoconjugate of the invention is used for detection, it may comprise a radioactive atom for scintigraphic studies, for example 99m-Tc or 111-In.

[0408] Labelled immunoconjugates of the invention are useful as imaging biomarkers and probes by the various methods and techniques of biomedical and molecular imaging such as: (i) MRI (magnetic resonance imaging); (ii) MicroCT (computerized tomography); (iii) SPECT (single photon emission computed tomography); (iv) PET (positron emission tomography) Chen et al Bioconjugate Chem. 15: 41-9 (2004); (v) bioluminescence; (vi) fluorescence; and (vii) ultrasound. Immunoscintigraphy is an imaging procedure in which antibodies labeled with radioactive substances are administered to an animal or human patient and a picture is taken of sites in the body where the antibody localizes (US 6528624). Imaging biomarkers may beobjectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, or pharmacological responses to a therapeutic intervention.

[0409] Another aspect of the present invention is a method of determining the presence of a target antigen in a sample suspected of containing the target antigen, wherein the method comprises exposing the sample to an immunoconjugate that binds to the target antigen and determining binding of the immunoconjugate to the target antigen in the sample, wherein the presence of such binding is indicative of the presence of the target antigen in the sample. Optionally, the sample may contain cells (which may be cancer cells) suspected of expressing the target antigen. The immunoconjugate employed in the method may optionally be detectably labeled, attached to a solid support, or the like.

[0410] Another embodiment of the present invention is directed to a method of diagnosing the presence of a tumor in a subject, wherein the method comprises (a) contacting a test sample comprising tissue cells obtained from the mammal with an immunoconjugate that binds to a target antigen and (b) detecting the formation of a complex between the immunoconjugate and the target antigen in the test sample, wherein the formation of a complex is indicative of the presence of a tumor in the mammal. Optionally, the immunoconjugate is detectably labeled, attached to a solid support, or the like, and / or the test sample of tissue cells is obtained from an individual suspected of having a cancerous tumor.

[0411] In some embodiments, the immunoconjugates of the present invention, including compositions comprising the aforementioned and / or provided herein are useful for detecting the presence of a target antigen, e.g., in vivo or in a biological sample. The immunoconjugates of the invention can be used in a variety of different assays, including but not limited to ELISA, beadbased immunoassays, and mass spectrometry.

[0412] In some embodiments, the immunoconjugates of the present invention are useful to quantitate target antigen amounts in a sample. In some embodiments, a biological sample is a biological fluid, such as whole blood or whole blood components including red blood cells, white blood cells, platelets, serum and plasma, ascites, vitreous fluid, lymph fluid, synovial fluid, follicular fluid, seminal fluid, amniotic fluid, milk, saliva, sputum, tears, perspiration, mucus, cerebrospinal fluid, urine and other constituents of the body that may contain the target antigen of interest. In various embodiments, the sample is a body sample from any animal. In some embodiments, the sample is from a mammal. In some embodiments, the sample is from a human subject. In some embodiments, the biological sample is serum from a clinical patient. In some embodiments, the biological sample is biopsy material. In some embodiments, the biological sample is biopsy material from a clinical patient. In some embodiments, the biological sample isserum from a clinical patient. In some embodiments, the biological sample is primary cell culture material. In some embodiments, the biological sample is primary cell culture material from a clinical patient. In some embodiments, the biological sample is from clinical patients or patients treated with a therapeutic antibody or antibodies that binds the same target antigen.

[0413] In some embodiments, the sample is from a mammal. In some embodiments, the sample is from a human subject, e.g., when measuring antigen expression in a clinical sample. In some embodiments, the biological sample is from clinical patients or a patient treated with a therapy / therapeutic (e.g., an antibody therapy targeting the same target antigen). In some embodiments, the biological sample is serum or plasma. In some embodiments, the biological sample is serum from a clinical patient. In some embodiments, the biological sample is biopsy material. In some embodiments, the biological sample is biopsy material from a clinical patient. In some embodiments, the biological sample is serum from a clinical patient. In some embodiments, the biological sample is primary cell culture material. In some embodiments, the biological sample is primary cell culture material from a clinical patient.

[0414] In some embodiments, compositions comprising ‘labeled’ immunoconjugates are provided. Labels include, but are not limited to, labels or moieties that are detected directly (such as fluorescent, chromophoric, electron-dense, chemiluminescent, and radioactive labels), as well as moieties, such as enzymes or ligands, that are detected indirectly, e.g., through an enzymatic reaction or molecular interaction. Exemplary labels include, but are not limited to, fluorophores such as rare earth chelates or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, luciferases, e.g., firefly luciferase and bacterial luciferase, luciferin, 2,3- dihydrophthalazinediones, horseradish peroxidase (HRP), alkaline phosphatase, J3 -galactosidase, glucoamylase, lysozyme, saccharide oxidases, e.g., glucose oxidase, galactose oxidase, and glucose-6-phosphate dehydrogenase, heterocyclic oxidases such as uricase and xanthine oxidase, coupled with an enzyme that employs hydrogen peroxide to oxidize a dye precursor such as HRP, lactoperoxidase, or microperoxidase, biotin / avidin, spin labels, bacteriophage labels, stable free radicals, and the like.

[0415] Conventional methods are available to bind these labels covalently to proteins or polypeptides. For instance, coupling agents such as dialdehydes, carbodiimides, dimaleimides, bis-imidates, bis-diazotized benzidine, and the like may be used to tag the immunoconjugates or antibody constructs of the invention with the above-described fluorescent, chemiluminescent, and enzyme labels (see e.g., US 3,645,090 (enzymes); US 3,940,475 (fhrorimetry), Hunter et al., Nature, 144:945 (1962); David et al., Biochemistry, 13: 1014-1021 (1974); Pain et al., J. Immunol. Methods, 40:219-230 (1981); Nygren, J. Histochem and Cytochem, 30:407-412 (1982).

[0416] The conjugation of such label, including the enzymes, to the immunoconjugate or antibody construct is a standard manipulative procedure for one of ordinary skill in immunoassay techniques (see e.g., O’Sullivan et al. “Methods for the Preparation of Enzyme-antibody Conjugates for Use in Enzyme Immunoassay,” in Methods in Enzymology, ed. J. Langone and H. Van Vunakis, Vol. 73 (Academic Press, New York, New York, 1981), pp. 147-166). Suitable commercially available labeled antibodies may also be used.

[0417] Following the addition of last labeled immunoconjugate, the amount of bound immunoconjugate is determined by removing excess unbound labeled immunoconjugate through washing and then measuring the amount of the attached label using a detection method appropriate to the label, and correlating the measured amount with the amount of the immunoconjugate of interest in the biological sample. For example, in the case of enzymes, the amount of color developed and measured will be a direct measurement of the amount of the immunoconjugate of interest present. Specifically, if HRP is the label, the color may be detected using the substrate TMD, using a 450 nm read wavelength and a 620 or 630 nm reference wavelength.

[0418] In one example, after an enzyme-labeled second antibody directed against the unlabeled immunoconjugate is washed from the immobilized phase, color or chemiluminescence is developed and measured by incubating the immobilized capture reagent with a substrate of the enzyme. Then the concentration of the antibody of interest is calculated by comparing with the color or chemiluminescence generated by the immunoconjugate of interest run in parallel.

[0419] In some embodiments, the method involves a bead -based immunoassay, an ELISA assay, or a mass spectrometric technique. The mass analyzers of such mass spectrometers include, but are not limited to, quadrupole (Q), time of flight (TOF), ion trap, magnetic sector or Fourier transform ion cyclotron resonance (FT-ICR) or combinations thereof. The ion source of the mass spectrometer should yield mainly sample molecular ions, or pseudo- molecular ions, and certain characterizable fragment ions. Examples of such ion sources include atmospheric pressure ionization sources, e.g., electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) and Matrix Assisted Laser Desorption Ionization (MALDI). ESI and MALDI are the two most commonly employed methods to ionize proteins for mass spectrometric analysis of small molecules, such as, e.g., by liquid chromatography mass spectrometry (LC / MS) (Lee, M., LC / MS Applications in Drug Development (2002) J. Wiley & Sons, New York). Another example is surface enhanced laser desorption ionization (SELDI). SELDI is a surface-based ionization technique that allows for high-throughput mass spectrometry. Typically, SELDI is used to analyze complex mixtures of proteins and other biomolecules. SELDI employs a chemically reactive surface such as a “protein chip” to interact with analytes, e.g., proteins, in solution. Suchsurfaces selectively interact with analytes and immobilize them thereon. Thus, the analytes of the invention can be partially purified on the chip and then quickly analyzed in the mass spectrometer. By providing multiple reactive moieties at different sites on a substrate surface, throughput may be increased.

[0420] In another aspect, the invention provides a method for detecting in a biological sample an antigen, the method comprising: (a) contacting the biological sample with an immunoconjugate described herein to allow forming an immunocomplex; (b) detecting or measuring the level of the immunoconjugate bound to the sample. In some embodiments, the immunoconjugate is immobilized to a solid support. In some embodiments, the immobilized immunoconjugate is conjugated to biotin and bound to a streptavidin coated microtiter plate.Kits and Articles of Manufacture of the Present Invention

[0421] Another aspect of the present invention is an article of manufacture containing materials useful for the treatment, prevention and / or diagnosis of diseases and disorders characterized by target antigen-expressing cells (e.g., a cancer cell). The article of manufacture of the invention comprises a container and a label or package insert on or associated with the container. Suitable containers include, for example, bottles, vials, syringes, etc. The containers may be formed from a variety of materials such as glass or plastic. The container holds a composition which is effective for treating, preventing and / or diagnosing the cancer condition and may have a sterile access port (for example the container may be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). At least one active agent in the composition is an immunoconjugate of the invention. The label or package insert indicates that the composition is used for treating cancer. The label or package insert will further comprise instructions for administering the immunoconjugate composition to the cancer patient. Additionally, the article of manufacture may further comprise a second container comprising a pharmaceutically-acceptable buffer, such as bacteriostatic water for injection (BWFI), phosphate- buffered saline, Ringer’s solution and dextrose solution. The article of manufacture may further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, needles, and syringes.

[0422] In another aspect, the invention provides a kit comprising any of the immunoconjugates described herein and an additional reagent or pharmaceutical device. In some further embodiments, the kit comprises a composition as provided herein (e.g., a pharmaceutical or diagnostic composition). Another aspect of the present invention is a kit useful for various purposes, e.g., target antigen-expressing cell killing; for target antigen-expressing cell detection; quantification, purification, or immunoprecipitation of target antigen from cells.

[0423] In some embodiments, the kit of the invention is an immunoassay kit for specifically detecting an antigen in a biological sample, comprising: (a) an immunoconjugate as described herein and / or a composition thereof; and (b) instructions for detecting said immunoconjugate. A target antigen detection assays of the present invention can be provided in the form of a kit. In some embodiments, such a kit comprises an immunoconjugate of the present invention, or a composition comprising the aforementioned, such as one described herein. The kit may further comprise a solid support for the capture reagents, which may be provided as a separate element or to which the capture reagents are already immobilized. For isolation and purification of a target antigen, the kit may contain an immunoconjugate of the invention coupled to beads (e.g., sepharose beads). The invention provides kits that contain an antibody for the detection and / or quantitation of target antigen in vitro, e.g., in an ELISA or a Western blot. In some embodiments, the capture reagents (e.g., the immunoconjugate of the invention) are coated on or attached to a solid material (e.g, to beads, a microtiter plate, or a comb). The detectable antibodies may be labeled antibodies detected directly or unlabeled antibodies that are detected by labeled antibodies directed against the unlabeled antibodies, such as, e.g., antibodies raised in a different species. Where the label is an enzyme, the kit will ordinarily include substrates and cofactors required by the enzyme; where the label is a fluorophore, a dye precursor that provides the detectable chromophore; and where the label is biotin, an avidin such as avidin, streptavidin, or streptavidin conjugated to HRP or P-galactosidase with MUG.

[0424] As with the article of manufacture of the invention, the kit of the invention comprises a container and a label or package insert on or associated with the container. The container holds a composition comprising at least one immunoconjugate of the invention. Additional containers may be included that contain, e.g., diluents and buffers, control immunoconjugates or antibodies. The label or package insert may provide a description of the composition as well as instructions for the intended in vitro or detection use. The kit also typically contains additives such as stabilizers, washing and incubation buffers, and the like for performing the assay method(s). The components of the kit will be provided in predetermined ratios, with the relative amounts of the various reagents suitably varied to provide for concentrations in solution of the reagents that substantially maximize the sensitivity of the assay(s). Particularly, the reagents may be provided as dry powders, usually lyophilized, including excipients, which on dissolution will provide for a reagent solution having the appropriate concentration for combining with the sample to be tested.

[0425] The present invention is further illustrated by the following non-limiting examples of immunoconjugates comprising the aforementioned structures and functions, in particular platforms having VHH polypeptides, a molecular weight between 60 and 110 kDa, a serum half-life of less than 96 hours, which in some embodiments exhibit enhanced stability during the temperatures required for certain radiolabeling processes relative to other antibody fragment platforms, and which in some embodiments exhibit decreased loss of targeting capacity due to radiolysis as compared to other possible delivery platforms.Certain definitions

[0426] In this description, certain specific details are set forth in order to provide a thorough understanding of various embodiments. However, one skilled in the art will understand that the embodiments provided may be practiced without these details. Unless the context requires otherwise, throughout the specification and claims which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense, that is, as “including, but not limited to.” As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and / or” unless the content clearly dictates otherwise. Further, headings provided herein are for convenience only and do not interpret the scope or meaning of the claimed embodiments.

[0427] The practice of the present invention will employ, unless otherwise indicated, conventional techniques of molecular biology (including recombinant techniques), microbiology, cell biology, biochemistry, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature, such as, “Molecular Cloning: A Laboratory Manual”, second edition (Sambrook et al., 1989); “Oligonucleotide Synthesis” (M. J. Gait, ed., 1984); “Animal Cell Culture” (R. I. Freshney, ed., 1987); “Methods in Enzymology” (Academic Press, Inc.); “Current Protocols in Molecular Biology” (F. M. Ausubel et al., eds., 1987, and periodic updates); “PCR: The Polymerase Chain Reaction”, (Mullis et al., ed., 1994); “A Practical Guide to Molecular Cloning” (Perbal Bernard V., 1988); “Phage Display: A Laboratory Manual” (Barbas et al., 2001). The skilled worker will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present invention. Indeed, the present invention is in no way limited to the methods and materials described. For purposes of the present invention, some terms are defined below.

[0428] As used in the specification and the appended claims, the terms “a,” “an” and “the” include both singular and the plural referents unless the context clearly dictates otherwise.

[0429] Throughout this specification, the term “including” is used to mean “including but not limited to.” “Including” and “including but not limited to” are used interchangeably.

[0430] The term “about” as used herein refers to the usual error range for the respective value readily known to the skilled person in this technical field. Reference to “about” a value orparameter herein includes (and describes) embodiments that are directed to that value or parameter per se. The term “about” when used before a numerical designation, e.g., a numerical temperature, time, amount, or concentration, including a range, indicates approximations which may vary by ± 10%, ± 5%, or ± 1%.

[0431] The term “amino acid residue” or “amino acid” includes reference to an amino acid that is incorporated into a protein, polypeptide, and / or peptide. The term “polypeptide” includes any polymer of amino acids or amino acid residues. The term “polypeptide sequence” refers to a series of amino acids or amino acid residues which physically comprise a polypeptide. A “protein” is a macromolecule comprising one or more polypeptides or polypeptide “chains.” A “peptide” is a small polypeptide of a size of 2 to 20 amino acid residues. The term “amino acid sequence” refers to a series of amino acids or amino acid residues which physically comprise a peptide or polypeptide depending on the length. Unless otherwise indicated, polypeptide and protein sequences disclosed herein are written from left to right representing their order from an amino terminus to a carboxy terminus.

[0432] The terms “amino acid,” “amino acid residue,” “amino acid sequence,” or polypeptide sequence include naturally occurring amino acids (including L and D isosteriomers) and, unless otherwise limited, also include known analogs of natural amino acids that can function in a similar manner as the common natural amino acids, such as selenocysteine, pyrrolysine, N- formylmethionine, gamma-carboxyglutamate, hydroxyprolinehypusine, pyroglutamic acid, and selenomethionine (see, e.g., Ho J et al., ACS Synth Biol 5: 163-71 (2016); Wang Y, Tsao M, Chembiochem 17: 2234-9 (2016)).

[0433] As used herein, the term “radioisotope” includes, but is not limited to, an alpha emitting isotope (interchangeably, a-emitting isotope), beta-emitting isotope (interchangeably, P- emitting isotope), and / or gamma-emitting isotope (interchangeably, y-emitting isotope), such as, e.g., any one of86Y,90Y,177Lu,186Re,188Re,89Sr,153Sm,225Ac,213Bi,213Po,212Bi,223Ra,224Ra,227Th,149Tb,68Ga,64Cu,67Cu,89Zr,137Cs,212Pb, and103Pd.

[0434] As used herein, the term “radioimmunoconjugate” refers to a molecular complex comprising (1) an immunoconjugate according to the present invention and (2) a radioisotope. In a preferred embodiment, the radioisotope is an a-emitting radioisotope. In another embodiment, the radioisotope is a P-emitting radioisotope. In another embodiment, the radioisotope is a y- emitting isotope. In another embodiment, the invention provides radioimmunoconjugates comprising a-emitting and P-emitting radioisotopes. The term “radioconjugate” is used interchangeably with the term “radioimmunoconjugate” herein. In one embodiment, theradioisotope is associated with a chelating agent of the radioimmunoconjugate. In one embodiment, the radioisotope is directly linked to the immunoconjugate.

[0435] As used herein, the term “immunoconjugate” refers to a molecular complex comprising an at least one antigen binding region derived from an antibody (e.g., variable regions or complementarity determining regions) further coupled to at least one non-antibody derived molecule, such as a chelator or cytotoxic agent. Non-antibody derived molecules may for example be conjugated to one or more lysine or cysteine resides of the antigen binding region or to a constant region coupled (by peptide linkage or otherwise) to the antigen binding region. In some embodiments, the immunoconjugate further comprises a chelating agent (interchangeably, “chelator”). In one embodiment, an immunoconjugate comprises an antibody construct of the invention linked directly or indirectly to a cytotoxic agent or radioisotope.

[0436] The immunoconjugates and radioimmunoconjugates described herein comprise antigen binding regions. These antigen binding regions can be derived from an “antibody.” The term “antibody” herein is used in the broadest sense and includes monoclonal antibodies, and includes intact antibodies and functional (antigen-binding) antibody fragments thereof, including fragment antigen binding (Fab) fragments, F(ab')2 fragments, Fab' fragments, Fv fragments, recombinant IgG (rlgG) fragments, single chain antibody fragments, including single chain variable fragments (sFv or scFv), and single domain antibodies (e.g., sdAb, sdFv, nanobody) fragments. The term encompasses genetically engineered and / or otherwise modified forms of immunoglobulins, such as intrabodies, peptibodies, chimeric antibodies, fully human antibodies, humanized antibodies, and heteroconjugate antibodies, multispecific, e.g., bispecific, antibodies, diabodies, triabodies, and tetrabodies, tandem di-scFv, tandem tri-scFv. Unless otherwise stated, the term “antibody” should be understood to encompass functional antibody fragments thereof. The term also encompasses intact or full- length antibodies, including antibodies of any class or sub-class, including IgG and sub-classes thereof, IgM, IgE, IgA, and IgD. The antibody can comprise a human IgGl constant region. The antibody can comprise a human IgG4 constant region.

[0437] The terms “complementarity determining region,” and “CDR,” which are synonymous with “hypervariable region” or “HVR,” are known in the art to refer to non-contiguous sequences of amino acids within antibody variable regions, which confer antigen specificity and / or binding affinity. In general, there are three CDRs in each heavy chain variable region (CDR-H1, CDR- H2, CDR-H3) and three CDRs in each light chain variable region (CDR-L1, CDR-L2, CDR-L3). “Framework regions” and “FR” are known in the art to refer to the non-CDR portions of the variable regions of the heavy and light chains. In general, there are four FRs in each full-lengthheavy chain variable region (FR-H1, FR-H2, FR-H3, and FR-H4), and four FRs in each full-length light chain variable region (FR-L1, FR-L2, FR-L3, and FR-L4). The precise amino acid sequence boundaries of a given CDR or FR can be readily determined using any of a number of well-known schemes, including those described by Kabat et al. (1991), “Sequences of Proteins of Immunological Interest,” 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD (“Kabat” numbering scheme), Al-Lazikani et al., (1997) JMB 273,927-948 (“Chothia” numbering scheme); MacCallum et al., J. Mol. Biol. 262:732-745 (1996), “Antibody-antigen interactions: Contact analysis and binding site topography,” J. Mol. Biol. 262, 732-745.” (“Contact” numbering scheme); Lefranc MP et al., “IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains,” Dev Comp Immunol, 2003 Jan;27(l):55-77 (“IMGT” numbering scheme); Honegger A and Pliickthun A, “Yet another numbering scheme for immunoglobulin variable domains: an automatic modeling and analysis tool,” J Mol Biol, 2001 Jun 8;309(3):657-70, (“Aho” numbering scheme); and Whitelegg NR and Rees AR, “WAM: an improved algorithm for modelling antibodies on the WEB,” Protein Eng. 2000 Dec;13(12):819-24 (“AbM” numbering scheme. In certain embodiments, the CDRs of the antibodies described herein can be defined by a method selected from Kabat, Chothia, IMGT, Aho, AbM, or combinations thereof.

[0438] The boundaries of a given CDR or FR may vary depending on the scheme used for identification. For example, the Kabat scheme is based on structural alignments, while the Chothia scheme is based on structural information. Numbering for both the Kabat and Chothia schemes is based upon the most common antibody region sequence lengths, with insertions accommodated by insertion letters, for example, “30a,” and deletions appearing in some antibodies. The two schemes place certain insertions and deletions (“indels”) at different positions, resulting in differential numbering. The Contact scheme is based on analysis of complex crystal structures and is similar in many respects to the Chothia numbering scheme.

[0439] The term “variable region” or “variable domain” refers to the domain of an antibody heavy or light chain that is involved in binding the antibody to antigen. The variable domains of the heavy chain and light chain (VH and VL, respectively) of a native antibody generally have similar structures, with each domain comprising four conserved framework regions (FRs) and three CDRs (See e.g., Kindt et al. Kuby Immunology, 6th ed., W.H. Freeman and Co., page 91(2007)). A single VH or VL domain may be sufficient to confer antigen-binding specificity. Furthermore, antibodies that bind a particular antigen may be isolated using a VH or VL domain from an antibody that binds the antigen to screen a library of complementary VL or VH domains,respectively (See e.g., Portolano et al., J. Immunol. 150:880-887 (1993); Clarkson et al., Nature 352:624-628 (1991)).

[0440] The antigen binding regions of the immunoconjugates described herein may be humanized. “Humanized” in reference to an immunoconjugate refers to an antigen binding region in which all or substantially all CDR amino acid residues are derived from non-human CDRs and all or substantially all FR amino acid residues are derived from human FRs. A humanized immunoconjugate optionally may include at least a portion of an antibody constant region derived from a human antibody.

[0441] Among the provided immunoconjugates are human immunoconjugates. A “human immunoconjugates” is an immunoconjugates possessing an antigen binding region with an amino acid sequence corresponding to that of an antibody produced by a human or a human cell, or non- human source that utilizes human antibody repertoires or other human antibody-encoding sequences, including human antibody libraries. The term excludes humanized forms of non- human antibodies comprising non-human antigen-binding regions, such as those in which all or substantially all CDRs are non-human.

[0442] The phrase “antigen binding arm”, as used herein, refers to a single polypeptide chain, comprising an “antigen binding region”, a hinge region, and a variant constant region. Other elements (e.g., a chelating agent; an imaging metal) may be attached to the antigen binding arm directly or through one or more linkers in compositions of the invention. Immunoconjugates of the invention comprise two antigen binding arms that are covalently linked together. In one embodiment, the antigen binding arms are linked through the hinge region. In one embodiment, the antigen binding arms are linked through an immunoglobulin heavy chain constant region. In one embodiment, the antigen binding arms are linked through the variant constant region. In one embodiment, the antigen binding arms are linked via a disulfide linkage (e.g., via a cysteine residue in a hinge region).

[0443] The phrase “antigen binding region”, as used herein, refers to the region of an immunoconjugate responsible for specific binding to an antigen, such region one or more antigen binding domains comprising complementarity determining regions, variable regions and framework regions, which may be derived from, modeled on, or may mimic, antibodies or fragments thereof, as are known by the person of ordinary skill in the art. In one embodiment, the “antigen binding region’ of an antigen binding arm contains one or two antigen binding domains. In a preferred embodiment, the “antigen binding region” of an antigen binding arm consists of a single antigen binding domain, which antigen binding domain is preferably a VHH polypeptide. In a preferred embodiment, the antigen binding regions of both antigen binding arms of animmunoconjugate independently consist of a single antigen binding domain, which antigen binding domain is preferably a VHH polypeptide, which VHH polypeptides are the same or different.

[0444] The term “VHH polypeptide” as used herein encompasses natural and synthetic compositions and refers to a polypeptide constituting a VHH fragment as it is known in the art, z.e., a polypeptide that constitutes a single domain heavy chain only variable domain fragment, or a polypeptide that structurally and functionally resembles a VHH fragment, as such structure is further described below and has the ability to specifically bind antigen is described below, and as both are well known in the art. In preferred embodiments, the VHH polypeptides comprise a heavy chain variable region comprising three heavy chain CDR’s; in one embodiment the VHH polypeptide is derived from a camelid; in another embodiment the VHH polypeptide is derived from a library; VHH polypeptides bind to antigens with specificity and high affinity. In a preferred embodiment, the VHH polypeptide is a single heavy chain variable domain comprising the arrangement: FR1, CDR1, FR2, CDR2, FR3, CDR3, and FR4. VHH polypeptides may be obtained, for example, as the antigen binding fragments of heavy chain only antibodies generated in vivo (e.g., in camelids). VHH polypeptides may also be obtained from synthetic libraries, e.g., phage display libraries. For example, see McMahon et al., Nature Structural & Molecular Biology | VOL 25 | MARCH 2018 | 289-296 Yeast surface display platform for rapid discovery of conformationally selective nanobodies: Moutel et al., eLife 2016;5:el6228 NaLi-HL A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies. De Genst E, Saerens D, Muyldermans S, Conrath K. Antibody repertoire development in camelids. Dev Comp Immunol. 2006;30(l-2):187-98. doi: 10.I0I6 / j.dci.2005.06.010. PMID: 16051357. Vincke C, Gutierrez C, Wernery U, Devoogdt N, Hassanzadeh-Ghassabeh G, Muyldermans S. Generation of single domain antibody fragments derived from camelids and generation of manifold constructs. Methods Mol Biol. 2012; 907: 145- 76. doi: 10.1007 / 978-l-61779-974-7_8. PMID: 22907350. Arbabi Ghahroudi M, Desmyter A, Wyns L, Hamers R, Muyldermans S. Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett. 1997 Sep 15;414(3):521-6. doi: 10.1016 / s0014-5793(97)01062-4. PMID: 9323027.

[0445] For VHH humanization, see, for example, Vincke C, Loris R, Saerens D, Martinez- Rodriguez S, Muyldermans S, Conrath K. General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold. J Biol Chem. 2009 Jan 30;284(5):3273-84. doi: 10.1074 / jbc.M806889200. Epub 2008 Nov 14. PMID: 19010777.

[0446] For VHH stability, see, for example, Kunz P, Flock T, Soler N, Zaiss M, Vincke C, Sterckx Y, Kastelic D, Muyldermans S, Hoheisel JD. Exploiting sequence and stability information for directing nanobody stability engineering. Biochim Biophys Acta Gen Subj. 2017 Sep;1861(9):2196-2205. doi: 10.1016 / j.bbagen.2017.06.014. Epub 2017 Jun 20. PMID: 28642127; PMCID: PMC5548252; Kunz P, Zinner K, Mucke N, Bartoschik T, Muyldermans S, Hoheisel JD. The structural basis of nanobody unfolding reversibility and thermoresistance. Sci Rep. 2018 May 21;8(1):7934. doi: 10.1038 / s41598-018-26338-z. PMID: 29784954; PMCID: PMC5962586.

[0447] A “linker” herein is also referred to as “linker sequence” “spacer” “tethering sequence” or grammatical equivalents thereof. A “linker” as referred herein connects two distinct molecules that by themselves possess target binding, catalytic activity, or are naturally expressed and assembled as separate polypeptides or comprise separate domains of the same polypeptide. For example, two distinct binding moieties or a heavy-chain / light-chain pair or an antigen binding region and an immunoglobulin heavy chain constant region. A number of strategies may be used to covalently link molecules together. Linkers described herein may be utilized to join a light chain variable region and a heavy chain variable region in an scFv molecule; or may be used to tether an scFv or other antigen binding fragment on the N- or C- terminus of an antibody heavy chain. These include but are not limited to polypeptide linkages between N- and C-termini of proteins or protein domains, linkage via disulfide bonds, and linkage via chemical cross-linking reagents. In one aspect of this embodiment, the linker is a peptide bond, generated by recombinant techniques or peptide synthesis.

[0448] An antibody that “binds” an antigen or epitope of interest is one that binds the antigen or epitope with sufficient affinity that is measurably different from a non-specific interaction. Specific binding can be measured, for example, by determining binding of a molecule compared to binding of a control molecule, which generally is a molecule of similar structure that does not have binding activity.

[0449] “Specific binding” refers to an antibody or immunoconjugate that is capable of binding antigen with sufficient affinity such that the antibody is useful as a diagnostic and / or therapeutic agent in targeting that antigen. In one embodiment, the extent of binding of an antibody to an unrelated protein is less than about 10% of the binding of the antibody to its antigen as measured, e.g., by a radioimmunoassay. An “antigen specific” antibody or immunoconjugate, as used herein, is one that specifically binds to the antigen with sufficient specificity and affinity to be useful in targeting a therapeutic, targeting diagnostic, or method of detecting the antigen in a biological sample from a subject. In some embodiments, an immunoconjugate or antibody construct or targetimaging complex or radioimmunoconjugate that binds to its target antigen has a dissociation constant (KD) of < 1 pM, < 100 nM, < 10 nM, < 1 nM, < 0.1 nM, < 0.01 nM, or < 0.001 nM (e.g., 10'8M or less, e.g., from 10'8M to 10'13M, e.g., from 10'9M to 10'13M). In some embodiments, an immunoconjugate or antibody construct or target imaging complex or radioimmunoconjugate of the present invention binds to multiple antigens, such as, e.g., an epitope conserved among homologs from different species, such as wherein the amino acid identity of the epitope is nonidentical in different species.

[0450] As used herein, the term “variant constant region” refers to a polypeptide comprising of a portion of an immunoglobulin heavy chain constant region that has been modified from native immunoglobulin amino acid sequence, preferably at from one to several amino acid positions. Unless otherwise specified herein, numbering of amino acid residues in the Fc region or constant region is according to the EU numbering system, also called the EU index, as described in Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD (1991). Modifications to Fc regions for various purposes are well known in the art. For example, see Kevin O. Saunders, Frontiers in Immunology, June 2019 | Volume 10 i Article 1296, titled “Conceptual Approaches to Modulating Antibody Effector Functions and Circulation Half-Life”'.

[0451] Percent (%) sequence identity with respect to a reference polypeptide sequence is the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the reference polypeptide sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways using available computer software. Appropriate parameters for aligning sequences are able to be determined, including algorithms needed to achieve maximal alignment over the full length of the sequences being compared. For purposes herein, however, % amino acid sequence identity values are generated using the sequence comparison computer program ALIGN-2. The ALIGN-2 sequence comparison computer program was authored by Genentech, Inc., and the source code has been filed with user documentation in the U.S. Copyright Office, Washington D.C., 20559, where it is registered under U.S. Copyright Registration No. TXU510087. The ALIGN-2 program is publicly available from Genentech, Inc., South San Francisco, Calif., or may be compiled from the source code. The ALIGN-2 program should be compiled for use on a UNIX operating system, including digital UNIX V4.0D. All sequence comparison parameters are set by the ALIGN-2 program and do not vary.

[0452] In situations where ALIGN-2 is employed for amino acid sequence comparisons, the % amino acid sequence identity of a given amino acid sequence A to, with, or against a given amino acid sequence B (which can alternatively be phrased as a given amino acid sequence A that has or comprises a certain % amino acid sequence identity to, with, or against a given amino acid sequence B) is calculated as follows: 100 times the fraction X / Y, where X is the number of amino acid residues scored as identical matches by the sequence alignment program ALIGN-2 in that program's alignment of A and B, and where Y is the total number of amino acid residues in B. It will be appreciated that where the length of amino acid sequence A is not equal to the length of amino acid sequence B, the % amino acid sequence identity of A to B will not equal the % amino acid sequence identity of B to A. Unless specifically stated otherwise, all % amino acid sequence identity values used herein are obtained as described in the immediately preceding paragraph using the ALIGN-2 computer program.

[0453] The term “cytotoxic agent” as used herein refers to a substance that inhibits or prevents a cellular function and / or causes cell death or destruction. Cytotoxic agents include, but are not limited to, radioa...

Claims

CLAIMSWhat is claimed is:

1. A compound of Formula (I), or a pharmaceutically acceptable salt thereof:Formula (I) wherein: at least one tertiary amide is present in the linear chain connecting R1to R8;R1is a radionuclide chelating moiety or a radionuclide complex thereof;X1is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-; m is 1, 2, 3, 4, 5, or 6; x is 0 or 1;R2is hydrogen, halogen, substituted or unsubstituted Ci-Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), -NRa-(substituted or unsubstituted Ci-Cealkyl), or substituted or unsubstituted Ci-Cioheteroalkyl, -©-(substituted or unsubstituted Ci- Cioheteroalkyl), -NRa-(substituted or unsubstituted Ci- Cioheteroalkyl), or C4- ©2opolyethylene glycol;X2is absent, -O-, -S-, -S(=O)2-, -NRa-, or -C(=O)N(Ra)-;L1is absent, Ci-Cioalkylene, ©2-Cioalkenylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, - S-, -S(=O)-, -S(=O)2-, -S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, - NRbC(=O)-, -C(=O)NRb-, -OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, - NRbC(=N-Rb)NRb-, or -NRbC(=O)O-;L2is absent,Y1is absent,n is 0 or 1; each R3and R6is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -C(=0)NH2, -C(=O)OH, or the side chain of an amino acid selected from thegroup consisting of: aspartic acid, glutamic acid, carboxyglutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with - C(=O)(unsubstituted or substituted Ci-C2oalkylene) or -C(=0)-C4-C2opolyethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl-3-bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or -C(=0)NH-(C4-C2opolyethylene glycol); y is 0 or 1;t is 0 or 1; each Ra, Rb, Rc, Rd, R2, R4, R4a, R5, R7and R7ais independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci- Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, -La-(Re), or R3; each u is independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; each Lais independently absent or Ci-Cealkylene;Reis substituted or unsubstituted Cs-Cncycloalkyl, substituted or unsubstituted C2- Cnheteroycloalkyl, substituted or unsubstituted phenyl, or substituted or unsubstituted pyridinyl; or R4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl; or R7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl;X4is absent, -CH2-, -O-, -S-, or -NRC-; q is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;R8is a moiety capable of covalently conjugating with a biologic targeting moiety (RB);2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein:X2is absent, -O-, -S-, -NRa-, or -C(=O)N(Ra)-; andL1is absent, Ci-Cioalkylene, or Ci-Cioheteroalkylene, wherein heteroalkylene is an alkylene where one internal carbon atom is replaced with -O-, -S-, -S(=O)-, -S(=O)2-, - S(=O)(=NH)-, -S(=O)(=NRb)-, -NRb-, -P(=O)ORb-, -NRbC(=O)-, -C(=O)NRb-, - OC(=O)NRb-, -NRbC(=O)NRb-, -NRbC(=N-CN)NRb-, -NRbC(=N-Rb)NRb-, or - NRbC(=O)O-.

3. The compound of claim 1 or 2, or a pharmaceutically acceptable salt thereof, wherein: RBis an antibody or fragment thereof, peptide, nucleic acid, or small molecule.

4. The compound of claim 1 or 2, or a pharmaceutically acceptable salt thereof: wherein: RBis an antibody or fragment thereof.

5. The compound of any one of claims 1-4, or a pharmaceutically acceptable salt thereof, wherein:R8is a moiety capable of conjugating with an amine (-NH2) or thiol (-SH) of an antibody or fragment thereof.The compound of any one of claims 1-4, or a pharmaceutically acceptable salt thereof, wherein: R8is a moiety capable of conjugating with an amine (-NH2) of an antibody or fragment thereof and is selected from:X5is absent, -O-, -S-, -S(=O)-, -S(=O)2-, -NR9-, -C(=O)-, -NR9C(=O)-, -C(=O)NR9-, -C(=O)O-, -OC(=O)-, -OC(=O)NR9-, -NR9C(=O)NR9-, -NR9C(=S)NR9-, -NR9C(=O)O-; and each R9is independently selected from hydrogen, and Ci-C4alkyl.

7. The compound of any one of claims 1-5, or a pharmaceutically acceptable salt thereof, wherein:

8. The compound of any one of claims 1-6, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (I) has the structure of Formula (la), (lb), (Ic), or a pharmaceutically acceptable salt thereof:Formula (Ic); wherein:X5is absent, -O-, -S-, -S(=O)-, -S(=O)2-, -NR9-, -C(=O)-, -NR9C(=O)-, -C(=O)NR9-, -C(=O)O-, -OC(=O)-, -OC(=O)NR9-, -NR9C(=O)NR9-, -NR9C(=S)NR9-, -NR9C(=O)O-; and each R9is independently selected from hydrogen, and Ci-C4alkyl.

9. The compound of any one of claims 1-4, or a pharmaceutically acceptable salt thereof, wherein:R8is a moiety capable of conjugating with a thiol (-SH) of an antibody or fragment thereof and is selected from:

10. The compound of any one of claims 1-4, or a pharmaceutically acceptable salt thereof, wherein:u is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12;X6is absent, -NR9- or -(CH2)g-NR9-; andR11is Cl, Br or I; and g is 1, 2, 3, or 4.

11. The compound of any one of claims 1-4, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (I) has the structure of Formula (Id), (le), (If), or (Ig), a pharmaceutically acceptable salt thereof:Formula (If), orFormula (Ig) wherein, X6is -NR9- or -(CH2)g-NR9-; and g is 1, 2, 3, or 4.

12. The compound of any one of claims 1-11, or a pharmaceutically acceptable salt thereof, wherein:R1is l,4,7,10-tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTA),1.4.7.10-tetraazacyclododecane-l,4,7-triacetic acid (D03A),1.4.7.10-tetraazacyclododecane-l,7-diacetic acid (D02A), а,a',a",a"'-tetramethyl-l,4,7,10-tetraazacyclododecane-l,4,7,10-tetraacetic acid (DOTMA),1,4,7, lO-tetrakis(carbamoylmethyl)- 1,4,7, 10-tetraazacyclododecane (DOTAM),1.4.7.10-tetraazacy clododecane- 1,4,7, 10-tetrapropionic acid (DOTPA),2,2',2"-(l 0-(2-amino-2-oxoethyl)- 1 ,4,7, 10-tetraazacy clododecane- 1 ,4,7-triyl)triacetic acid, б,6'-(((pyridine-2,6-diylbis(methylene))bis((carboxymethyl)azanediyl))bis(methylene))- dipicolinic acid (F pypa),6,6',6",6"'-(((pyridine-2,6-diylbis(methylene))bis(azanetriyl))-tetrakis(methylene))- tetrapicolinic acid (JHUpydpa),2,2',2”-(l,4,7-triazacyclononane-l,4,7-triyl)triacetic acid (NOTA), 6,6'-((ethane-l,2-diylbis((carboxymethyl)azanediyl))bis(methylene))dipicolinic acid (T octapa), or3,6,9, 12-tetrakis(carboxymethyl)-3, 6,9, 12-tetraazatetradecanedioic acid (TTHA), or a radionuclide complex thereof.

13. The compound of any one of claims 1-11, or a pharmaceutically acceptable salt thereof, wherein:or a radionuclide complex thereof.

14. The compound of any one of claims 1-11, or a pharmaceutically acceptable salt thereof, wherein:or a radionuclide complex thereof.

15. The compound of any one of claims 1-14, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (I) has the structure of Formula (II), or a pharmaceutically acceptable salt thereof:Formula (II).

16. The compound of any one of claims 1-14, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (I) has the structure of Formula (V), or a pharmaceutically acceptable salt thereof:Formula (V).

17. The compound of any one of claims 1-16, or a pharmaceutically acceptable salt thereof, wherein:

18. The compound of claim 17, or a pharmaceutically acceptable salt thereof, wherein:

19. The compound of claim 17, or a pharmaceutically acceptable salt thereof, wherein:R4and R4aare independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re); orR4and R4aare taken together with the intervening atoms connecting R4to R4ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl.

20. The compound of any one of claims 1-16, or a pharmaceutically acceptable salt thereof, wherein:Y1is absent and L3is absent.

21. The compound of any one of claims 1-20, or a pharmaceutically acceptable salt thereof, wherein:

22. The compound of any one of claims 1-20, or a pharmaceutically acceptable salt thereof, wherein:R7and R7aare independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re); orR7and R7aare taken together with the intervening atoms connecting R7to R7ato form a substituted or unsubstituted C2-Ci2heteroycloalkyl.

23. The compound of claim 15, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (II) has the structure of Formula (III), or a pharmaceutically acceptable salt thereof:Formula (III) wherein,X2is absent, -CH2-, or -O-; and X3is absent, -CH2-, or -O-.

24. The compound of claim 15, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (II) has the structure of Formula (IV), or a pharmaceutically acceptable salt thereof:Formula (IV) wherein,X2is absent, -CH2-, or -O-;X3is absent, -CH2-, or -O-; and25. The compound of any one of claims 15, 23, or 24, or a pharmaceutically acceptable salt thereof, wherein:or a radionuclide complex thereof.

26. The compound of claim 16, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (V) has the structure of Formula (VI), or a pharmaceutically acceptable salt thereof:Formula (VI) wherein,X2is absent, -CH2-, or -O-; andX3is absent, -CH2-, or -O-.

27. The compound of claim 16, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (I) has the structure of Formula (VII), or a pharmaceutically acceptable salt thereof:Formula (VII) wherein,X2is absent, -CH2-, or -O-;X3is absent, -CH2-, or -O-; and28. The compound of any one of claims 16, 26, or 27, or a pharmaceutically acceptable salt thereof, wherein:or a radionuclide complex thereof.

29. The compound of any one of claims 23-28, or a pharmaceutically acceptable salt thereof, wherein:X3is -CH2- and X4is -O-; or X3is -O- and X4is -CH2-.

30. The compound of any one of claims 23-28, or a pharmaceutically acceptable salt thereof, wherein:

31. The compound of any one of claims 23-28, or a pharmaceutically acceptable salt thereof, wherein:

32. The compound of any one of claims 23-28, or a pharmaceutically acceptable salt thereof, wherein:

34. The compound of claim 32, or a pharmaceutically acceptable salt thereof, wherein:

35. The compound of any one of claims 1-34, or a pharmaceutically acceptable salt thereof, wherein:L1is Ci-Cioalkylene and X1is absent or -O-.

36. The compound of any one of claims 1-34, or a pharmaceutically acceptable salt thereof, wherein:L1is C2-Cioalkenylene and X1is absent or -O-.

37. The compound of any one of claims 1-36, or a pharmaceutically acceptable salt thereof, wherein:

38. The compound of any one of claims 1-37, or a pharmaceutically acceptable salt thereof, wherein:R3is independently hydrogen, -C(=0)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carb oxy glutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with -C(=O)(unsubstituted or substituted Ci-C2oalkylene) or - C(=0)-C4-C2opoly ethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl-3- bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or - C(=0)NH-(C4-C2opolyethylene glycol).

39. The compound of any one of claims 1-36, or a pharmaceutically acceptable salt thereof, wherein:The compound of any one of claims 1-36, or a pharmaceutically acceptable salt thereof, wherein: L2is absent.

41. The compound of any one of claims 1-34, or a pharmaceutically acceptable salt thereof, wherein:

42. The compound of claim 41, or a pharmaceutically acceptable salt thereof, wherein:

43. The compound of any one of claims 1-42, or a pharmaceutically acceptable salt thereof, wherein: each R4and R7is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re).

44. The compound of claim 43, or a pharmaceutically acceptable salt thereof, wherein: each R4and R7is independently hydrogen, substituted or unsubstituted Ci-Cealkyl, or - La-(Re).

45. The compound of any one of claims 1-42, or a pharmaceutically acceptable salt thereof, wherein:R4is hydrogen; andR7is substituted or unsubstituted Ci-Cealkyl, substituted, or substituted or unsubstituted Ci-Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re).

46. The compound of claim 45, or a pharmaceutically acceptable salt thereof, wherein: R4is hydrogen; andR7is substituted or unsubstituted Ci-Cealkyl, or -La-(Re).

47. The compound of any one of claims 1-42, or a pharmaceutically acceptable salt thereof, wherein:R4is substituted or unsubstituted Ci-Cealkyl, substituted or unsubstituted Ci- Ceheteroalkyl, C4-C2opolyethylene glycol, -(CH2CH2O)U-CH3, or -La-(Re); andR7is hydrogen.

48. The compound of claim 47, or a pharmaceutically acceptable salt thereof, wherein: R4is substituted or unsubstituted Ci-Cealkyl, or -La-(Re); andR7is hydrogen.

49. The compound of any one of claims 1-48, or a pharmaceutically acceptable salt thereof, wherein: each Reis independently selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, bicyclof 1.1.1 ]pentanyl, bicyclo[2.1.1 ]hexanyl, bicyclo[3.1.1 ]heptanyl, bicyclo[2.2.2]octanyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, 2- oxabicyclofl .

1. l]pentanyl, 2-oxabicyclo[2.

1. l]hexanyl, 2-oxabicyclo[2.2.2]octanyl, azetidinyl, pyrrolidinyl, piperidinyl, pyridinyl, phenyl, and adamantyl, wherein each Reis independently optionally substituted.

50. The compound of any one of claims 1-48, or a pharmaceutically acceptable salt thereof, wherein:each Reis independently selected fromwherein each Reis independently optionally substituted.

51. The compound of any one of claims 1-50, or a pharmaceutically acceptable salt thereof, wherein: each Lais independently absent, -CH2-, -CH2CH2-, -CH2CH2CH2-, -CH2CH2CH2CH2-,- CH2CH2CH2CH2CH2-, or -CH2CH2CH2CH2CH2CH2-.

52. The compound of any one of claims 1-42, or a pharmaceutically acceptable salt thereof, wherein:(CH2CH2O)-CH3, -(CH2CH2O)2-CH3, -(CH2CH2O)3-CH3, -(CH2CH2O)4-CH3, - (CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, - (CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)II-CH3, or -(CH2CH2O)I2-CH3.

53. The compound of claim 52, or a pharmaceutically acceptable salt thereof, wherein:(CH2CH2O)4-CH3, -(CH2CH2O)5-CH3, -(CH2CH2O)6-CH3, -(CH2CH2O)7-CH3, -(CH2CH2O)8-CH3, -(CH2CH2O)9-CH3, -(CH2CH20)IO-CH3, -(CH2CH2O)II-CH3, or - (CH2CH2O)12-CH3.

54. The compound of any one of claims 1-42, or a pharmaceutically acceptable salt thereof, wherein:R4or R7is not hydrogen; orR4and R7are the same; orR4and R7are different.

55. The compound of any one of clams 23-38, or a pharmaceutically acceptable salt thereof, wherein:

56. The compound of any one of claims 1-55, or a pharmaceutically acceptable salt thereof, wherein: each R6is independently hydrogen, -C(=0)NH2, -C(=O)OH, or the side chain of an amino acid selected from the group consisting of: aspartic acid, glutamic acid, carb oxy glutamic acid, glutamine, asparagine, lysine, homolysine, ornithine, serine, homoserine, threonine, arginine, methyl arginine, citrulline, homoarginine, methyl homoarginine, norarginine, canavanine, methyl citrulline, methyl norarginine, and methyl canavanine, wherein any free amine (-NH2) of R3or R6is independently optionally substituted with -C(=O)(unsubstituted or substituted Ci-C2oalkylene) or - C(=0)-C4-C2opoly ethylene glycol; wherein any free carboxylic acid (-CO2H) of R3or R6is independently optionally replaced with -C(=O)NH-(2,4,6-trimethyl-3- bromophenyl), -C(=O)NH-( unsubstituted or substituted Ci-C2oalkylene), or - C(=0)NH-(C4-C2opolyethylene glycol).

57. The compound of any one of claims 1-55, or a pharmaceutically acceptable salt thereof, wherein:

58. The compound of any one of claims 23 or 37, or a pharmaceutically acceptable salt thereof, wherein:

59. The compound of any one of claims 1-58, or a pharmaceutically acceptable salt thereof, wherein:R2is hydrogen, halogen, substituted or unsubstituted Ci-Cealkyl, -©-(substituted or unsubstituted Ci-Cealkyl), or C4-C2opolyethylene glycol.

60. The compound of any one of claims 1-59, or a pharmaceutically acceptable salt thereof, wherein: m is 1, 2, 3, or 4.

61. The compound of claim 60, or a pharmaceutically acceptable salt thereof; wherein: m is 1.

62. The compound of claim 15, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula (II) has the structure of Formula (V), or a pharmaceutically acceptable salt thereof:

63. The compound of claim 62, or a pharmaceutically acceptable salt thereof, wherein:

64. The compound of claim 62 or 63, or a pharmaceutically acceptable salt thereof, wherein:

65. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the compound is:,0or a pharmaceutically acceptable salt thereof.

66. The compound of any one of claims 1-65, or a pharmaceutically acceptable salt thereof, wherein: the radionuclide is a lanthanide or an actinide.

67. The compound of any one of claims 1-65, or a pharmaceutically acceptable salt thereof, wherein: the radionuclide is a diagnostic or therapeutic radionuclide.

68. The compound of any one of claims 1-65, or a pharmaceutically acceptable salt thereof, wherein: the radionuclide is an Auger electron-emitting radionuclide, a-emitting radionuclide, P-emitting radionuclide, or y-emitting radionuclide.

69. The compound of any one of claims 1-65, or a pharmaceutically acceptable salt thereof, wherein: the radionuclide is a-emitting radionuclide.

70. The compound of any one of claims 1-65, or a pharmaceutically acceptable salt thereof, wherein: the radionuclide is 225-actinium (225Ac), 213-bismuth (213Bi), 223-Radium (223Ra), 212-lead (212Pb), 111-indium (H 1In), 67-gallium (67Ga), 68-gallium (68Ga), 99m- technetium (99mTc), 90-yttrium (90Y), 177-lutetium (177Lu), 186-rhenium (186Re), 188- rhenium (188Re), 64-copper (64Cu), 67-copper (67Cu), 153-samarium (153Sm), 89- strontium (89Sr), 198-gold (198Au), 169-Erbium (169Er), 165-dysprosium (165Dy), 99m- technetium (99mTc), 89-zirconium (89Zr), or 52-manganese (52Mn).

71. The compound of any one of claims 1-65, or a pharmaceutically acceptable salt thereof, wherein: the radionuclide is 225-actinium (225Ac).

72. The compound of any one of claims 1-71, or a pharmaceutically acceptable salt thereof, wherein the targeting moiety is a polypeptide comprising an antigen binding region and an immunoglobulin heavy chain constant region, wherein the molecular weight of the polypeptide is between 60 and 110 kDa.

73. The compound of any one of claims 1-71, or a pharmaceutically acceptable salt thereof, wherein the targeting moiety binds to Delta-like protein 3 (DLL3), DLL1, folate receptor alpha (FOLR1), Folate Receptor a (FRa), human epidermal growth factor receptor 2 (HER2), HER3, epidermal growth factor receptor (EGFR) insulin-like growth factor 1 (IGF-1) receptor, vascular endothelial growth factor (VEGF), or Tumor-associated calcium signal transducer 2 (TROP2), Angiopoietin, BCMA, CD 19, CD20, CD22, CD25 (IL2-R), CD30, CD33, CD37, CD38, CD52, CD56, CD123 (IL-3R), cMET, DLL / Notch, EpCAM, FGF / FGF-R, GD2, Mesothelin, Nectin-4, PDGFRa, RANKL, SLAMF7, Claudin 18.2, DKK1, B7-H3, NaPi2B, GCC, GCP3, GD2, CLDN6, CLDN18.2, STEAP1, STEAP2, LIV1, MUC16, MUC1, SLC44A, TM4sFl, NTSR1, B7-H3, B7H7, KAAG1, GDPCP1, GD2, LAT1, Integrin beta 6, CAIX, Flt3, EphA2, or TROP2.

74. A pharmaceutical composition comprising a compound of any one of claims 1-73, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient.

75. The pharmaceutical composition of claim 74, wherein the pharmaceutical composition is formulated for administration to a mammal by intravenous administration.

76. A method of treating cancer in a mammal comprising administering to the mammal the compound of any one of claims 1-73 that is conjugated to a targeting moiety.

77. The method of claim 76, wherein the mammal is a human.

78. The method of claim 76 or 77, wherein the cancer comprises a glioma, thyroid cancer, lung cancer, colorectal cancer, stomach cancer, liver cancer, pancreatic cancer, renal cancer, prostate cancer, testis cancer, breast cancer, cervical cancer, endometrial cancer, ovarian cancer, or melanoma.

79. A method for identifying tissues or organs in a mammal with a tumor comprising: i) administering to the mammal a radionuclide complex of the compound of any one of claims 1-73 conjugated to a targeting moiety, and ii) performing positron emission tomography (PET) analysis, single-photon emission computerized tomography (SPECT), or magnetic resonance imaging (MRI); wherein the tumor expresses receptors that are targeted by the targeting moiety; wherein the radionuclide is a diagnostic radionuclide.

0. The method of any claim 79, wherein: step (ii) is initiated after an amount of time following step (i) sufficient for interaction between the radionuclide complex of the compound of any one of claims 1-66 conjugated to the targeting moiety.

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