Method and device for producing a chlorella suspension

Abstract

The inventions relate to the cultivation of chlorella. Producing a suspension of chlorella includes cultivating chlorella on a growth medium containing, per 1 l of water: 0.2 g of ammonium nitrate; 0.1 ml of a 15% solution of ammonium phosphate; 0.15 ml of a 1% solution of ferrous chloride; 0.5 ml of a 0.01% solution of cobaltous nitrate; 0.5 ml of a 0.01% solution of cuprous sulphate; 5-10 ml of a solution of carbon dioxide gas (pH 3-5). Carbon dioxide gas is prepared using naked oats doused with the solution of growth medium reagents. A chlorella suspension stock culture and the growth medium solution are introduced into a glass vessel, and hydroplasma in an amount of 1-10 grams per 12 litres of growth medium and a hermetically sealed capsule containing metaplasma in an amount of 2-20 grams per 12 litres of growth medium are added. The chlorella is cultivated in a device in which it is irradiated by a source of light for 4 light cycles, wherein the light source is connected to an alternating positron current. The inventions make it possible to produce a chlorella suspension with an improved chemical composition and enhanced biological activity.

Description

[0001] METHOD FOR OBTAINING CHLORELLA SUSPENSION AND DEVICE FOR OBTAINING

[0002] CHLORELLA SUSPENSIONS

[0003] DESCRIPTION

[0004] The invention relates to methods for breeding microorganisms and obtaining biologically active additives from algae [A23K 10 / 00, A23K 10 / 10, A23K 10 / 12, A23K 10 / 18, A01G 33 / 00, C12N 1 / 00, C12N 1 / 12].

[0005] The prior art discloses a patent for a METHOD FOR GROWING MICROALGAE BIOMASS AND A SETUP FOR ITS IMPLEMENTATION [RU 2718515 C1, published: 04 / 08 / 2020], in which a method for growing microalgae biomass includes preparing a mineral nutrient medium, adding an initial culture of a microalgae strain, pouring the resulting culture mixture into a system of sequentially located bioreactors made in the form of horizontally oriented chambers made of light-transmitting material, illuminating the culture mixture using vertically installed artificial light sources, removing the resulting suspension into a container for natural sedimentation and then removing the resulting sedimented biomass as a target product, characterized in that

[0006] - the prepared mineral nutrient medium has the following composition:

[0007] - ammonium nitrate (34% solution) 0.14 ml ammophos (15% solution) 0.10 ml iron chloride (1% solution) 0.15 ml cobalt nitrate (0.1% solution) 0.10 ml copper sulfate (0.1% solution) 0.10 ml drinking water 1000 ml,

[0008] - biomass is grown in a single chamber of a bioreactor, made in the form of a vertically oriented parallelepiped,

[0009] - illumination of the culture mixture is carried out by artificial light sources installed on the inner side of one of the wide walls of the bioreactor chamber in horizontally oriented rows along the height of the bioreactor chamber,

[0010] - the illumination of the culture mixture is carried out cyclically,

[0011] - during all microalgae growing cycles, the pH value of the culture mixture is maintained in the range of 8.5-9.5 by adding to the culture mixture at the beginning of each light cycle a solution with lactic acid bacteria, the pH value of which is selected in the range of 4.0-5.0, in an amount of 1-3 ml per 1 l of the culture mixture, while the illumination of the culture mixture is carried out during 4 light cycles, each of which includes 10 hours of illumination and the subsequent 2 hours of no illumination, during the illumination period the temperature of the culture mixture is maintained in the range of 26-30 °C, and during the period of no illumination - in the range of 24-26 °C.

[0012] The installation for growing microalgae biomass in the claimed patent contains sequentially arranged bioreactors made in the form of horizontal chambers made of transparent material, equipped with mixing means, connected by a discharge line to a container of finished microalgae biomass, a bioreactor of a solution with lactic acid bacteria, connected at the outlet to the microalgae bioreactors, a unit for preparing a nutrient medium, connected at the outlet to the microalgae bioreactors and the bioreactor of a solution with lactic acid bacteria, artificial light sources in the form of electric lamps equipped with a cooling system, washing heads connected to a system for preparing a washing liquid, pumps and shut-off and control devices, wherein the microalgae bioreactor chamber is made in the form of a vertically oriented parallelepiped made of transparent material,artificial light sources are installed on the inside of one of the wide walls of the chamber perpendicular to it in horizontally oriented n rows along the height of the chamber, washing heads are installed on the inside of the opposite wall of the bioreactor chamber in such a way that 4 washing heads are symmetrically placed around each artificial light source, the installation is equipped with a programmable automatic system for monitoring and controlling the specified parameters of temperature, volume, dosage, loading, unloading of the solution and the finished product.

[0013] The closest in technical essence is the METHOD OF CULTIVATION OF PLANKTONIC CHLORELLA [RU 2685955 C1, published: 23.04.2019], including its cultivation on a nutrient medium consisting of mineral and organic components, characterized in that the cultivation is carried out in three stages and simultaneously in the mode of alternating two light and one dark phase of photosynthesis for 10 ... 4 ... 10 hours, respectively, at each stage under illumination with DRI-250 lamps or sunlight, a culture temperature of 28-30 ° C, on a nutrient medium consisting of mineral and organic components, which, together with the original seed material of the culture, is taken in a ratio of 1: 1, while for each subsequent stage the original material is the suspension obtained at the previous stage of cultivation, and the stages themselves differ from each other in the introduction of organic components into the nutrient medium at the following content per liter of tap water:

[0014] - mineral

[0015] 1. Ammonium nitrate 0.4 g;

[0016] 2. Ammophos, 15% solution 0.2 ml;

[0017] 3. Iron chloride, 1% solution 0.15 ml;

[0018] 4. Cobalt nitrate, 0.01% solution 0.5 ml;

[0019] 5. Copper sulfate, 0.01% solution 0.5 ml;

[0020] 6. Magnesium sulfate 0.3 g;

[0021] 7. Potassium sulfate, 12% solution 0.5 ml;

[0022] - organic

[0023] 1. Filtrate of naked oat infusion (pH 3.5-5.0), cultivation stage: I - 35.0 ml; II - 50.0 ml; III - 60.0 ml;

[0024] 2. Fugate of grain post-alcohol stillage (pH 4.2-4.5), cultivation stage: I - 50.0 ml; II - 60.0 ml; III - 85.0 ml.

[0025] The main technical problem of the analogue and prototype is low biological activity and low nutrient content.

[0026] The objective of the invention is to eliminate the shortcomings of the analogue and prototype.

[0027] The technical result of the invention is the production of a chlorella suspension with improved chemical composition and biological activity.

[0028] The specified technical result is achieved due to the fact that the method for obtaining a chlorella suspension, including culturing chlorella on a nutrient medium consisting of mineral and organic components by photosynthesis, characterized in that the following reagents and carbon dioxide are used to prepare the nutrient medium per 1000 ml of tap water: ammonium nitrate - 0.2 g, ammophos, 15% solution - 0.1 ml, iron chloride, 1% solution - 0.15 ml, cobalt nitrate, 0.01% solution - 0.5 ml, copper sulfate, 0.01% solution - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml, while naked oats are used as raw material for preparing carbon dioxide, which is poured with a solution of reagents prepared for the nutrient medium, cultivation chlorella is carried out in a device for obtaining a chlorella suspension, for which the mother culture of the chlorella suspension and a nutrient solution are added to the container,hydroplasma at the rate of 1-10 grams per 12 liters and a hermetically sealed capsule with metaplasma at the rate of 2-20 grams per 12 liters of nutrient medium, and irradiation with a light source is carried out during 4 light cycles, while in normal mode each of the light cycles includes 16 hours of irradiation with a pause between light cycles of 8 hours, and with the express method each of the light cycles includes 10 hours of irradiation with a pause between light cycles of 2 hours.

[0029] In particular, the mother culture of chlorella suspension and the nutrient solution are introduced into the container in a ratio of 1:4.

[0030] In particular, the nutrient medium is prepared in accordance with the order of addition of reagents and carbon dioxide.

[0031] In particular, the solution of reagents for preparing the nutrient medium is taken in a volume necessary to exceed the oat level in the container by two times.

[0032] In particular, a container with a solution of reagents and oats is kept at a temperature of 23-30°C until the acidity level reaches a pH of 3 to 5.

[0033] A method for producing a chlorella suspension, including culturing chlorella on a nutrient medium consisting of mineral and organic components by photosynthesis, characterized in that the following reagents and carbon dioxide are used to prepare the nutrient medium per 1000 ml of tap water: ammonium nitrate - 0.2 g, ammophos, 15% solution - 0.1 ml, iron chloride, 1% solution - 0.15 ml, cobalt nitrate, 0.01% solution - 0.5 ml, copper sulfate, 0.01% solution - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml, while naked oats are used as raw material for preparing carbon dioxide, which is filled with a solution of reagents prepared for the nutrient medium, chlorella is cultivated in a device for obtaining a suspension chlorella, for which the mother culture of chlorella suspension and nutrient solution are added to the container,hydroplasma at the rate of 1-10 grams per 12 liters and a hermetically sealed capsule with metaplasma at the rate of 2-20 grams per 12 liters of nutrient medium, and irradiation with a light source is carried out in light cycles, while in normal mode the light cycle consists of 16 hours of irradiation and 8 hours of pause, and with the express method the light cycle consists of 10 hours of irradiation and 2 hours of pause, while the draining of the finished chlorella suspension is carried out daily with the replacement of the drained volume with a nutrient solution.

[0034] In particular, the mother culture of chlorella suspension and the nutrient solution are introduced into the container in a ratio of 3:1.

[0035] In particular, the nutrient medium is prepared in accordance with the order of addition of reagents and carbon dioxide.

[0036] In particular, the solution of reagents for preparing the nutrient medium is taken in a volume necessary to exceed the oat level in the container by two times.

[0037] In particular, a container with a solution of reagents and oats is kept at a temperature of 23-30°C until the acidity level reaches a pH of 3 to 5.

[0038] In particular, the chlorella suspension is cultivated at a temperature of 28-30°C. The device for obtaining the chlorella suspension comprises at least one vertical glass container equipped with a shut-off valve, a lamp with at least one light source powered by a positron alternating current and facing the side wall of the container, a controller configured to control the switching on / off of the lamp, a sensor for monitoring the temperature of the chlorella suspension in the container, heating and cooling elements configured to maintain the temperature of the nutrient medium in the container specified in the controller, a photo sensor configured to monitor the level of the light flux from the lamp passing through the container, a sensor for monitoring the level inside the container, an interface module, an indicator unit and controls for the device are connected to the controller.

[0039] In particular, the container is made in the form of a rectangular parallelepiped, a polygonal rectangular prism or an arc-shaped one in plan, while the wall of the arc-shaped container facing the lamp is made convex away from the lamp.

[0040] In particular, the tank is made annular in plan, while the lamp is mounted in the center of the tank. In particular, the light center of the lamp is located in the center of the thickness of the nutrient medium. In particular, DRL or DNaT lamps are used as a light source in the lamp.

[0041] In particular, the shut-off valves of the tank at the bottom for complete drainage of the nutrient medium from the tank and on one of the side walls of the tank with a gap from the bottom of the tank for drainage of the chlorella suspension.

[0042] In particular, the shut-off valves are equipped with valves controlled by a controller.

[0043] In particular, the heating and cooling elements can be made in the form of Peltier elements.

[0044] In particular, the indication unit and / or control elements can be combined with an interface module, implemented in the form of a touch screen for indicating and displaying operating modes on the screen of the interface module, while the control elements are implemented in the form of touch buttons displayed on the screen.

[0045] Brief description of the drawings.

[0046] Fig. 1 shows a top view of the device for obtaining a chlorella suspension.

[0047] Fig. 2 shows a side view of the device for obtaining a chlorella suspension.

[0048] Fig. 3 shows a structural diagram of a device for obtaining a chlorella suspension.

[0049] The following are indicated on the figures: 1 - containers, 2 - lamp, 3 - support surface, 4 - taps, 5 - controller, 6 - relay, 7 - temperature sensor, 8 - photo sensor, 9 - valve, 10 - level sensor, 11 - interface module, 12 - indicator unit, 13 - controls, 14 - heating element, 15 - cooling element.

[0050] Implementation of the invention.

[0051] Chlorella is a representative of green algae - microscopic aquatic plants. The essence of the claimed invention is a method (technology) for obtaining a chlorella suspension, taking into account the biology and morphological features of the species and the preparation for use as a biologically active supplement by humans and animals of a suspension of live chlorella using Chlorella vulgaris, for example, Ch lorella vulgaris IFR No. C-111.

[0052] Planktonic strains of Chlorella vulgaris are distinguished by a high degree of utilization of light energy (efficiency of photosynthetically active radiation 3.6%) and chemical composition of the cell in terms of protein content, essential amino acids, vitamins, a set of microelements and biologically active substances, which cannot be compared not only with aquatic but also with terrestrial plants.

[0053] The preparation of a chlorella suspension from the Chlorella vulgaris strain IFR No. C-111 is carried out in several stages.

[0054] At the first stage, water treatment is carried out, for which a low-mineralized aqueous solution or drinking water with a salt concentration of 0 to 0.3 grams per liter is successively treated with ultraviolet light with a power of 0.1 W per l / h, permanent magnets, with a magnetic field density of 120 Gs per l / h and fed into a reactor on flat electrodes without a separating membrane, where in the cathode chamber of the reactor the solution is exposed to electric current in circulation mode using a circulation circuit while maintaining the pH value of the electrolyte in the circulation circuit.Then the solution is fed into reactors on coaxially located electrodes with a separating membrane, while in each reactor on coaxially located electrodes the anode has a diameter of 20 mm, the cathode is made of titanium, the diameter of the cathode is 30 mm, the separating membrane has a diameter of 25 mm and is made of a mixture of aluminum oxide and zirconium, while the length of the working part of the reactor on coaxially located electrodes is 250 mm, in reactors on coaxially located electrodes with a separating membrane, the synthesis of the final solution is realized by sequential circulation.

[0055] At the second stage, the nutrient medium is prepared to obtain a chlorella suspension.

[0056] In one embodiment, the following reagents and carbon dioxide are used to prepare the nutrient medium per 1000 ml of tap water: ammonium nitrate - 0.2 g, ammophos, 15% solution - 0.1 ml, iron chloride, 1% solution - 0.15 ml, cobalt nitrate, 0.01% solution - 0.5 ml, copper sulfate, 0.01% solution - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml.

[0057] In another embodiment, the following reagents and carbon dioxide are used to prepare the nutrient medium per 1000 ml of tap water: nitrogen-phosphorus solution - 0.3 ml, iron chloride, 1% solution - 0.15 ml, copper-cobalt solution, 0.01% - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml. The nutrient medium is prepared observing the sequence of adding reagents and carbon dioxide. After adding each ingredient, the nutrient medium is thoroughly mixed. When preparing the nutrient medium, before adding the carbon dioxide solution, the state of the solution is monitored and it is ensured that there are no flakes, sediment or opalescence in the nutrient medium solution being prepared.

[0058] To prepare a nitrogen-phosphorus solution according to the second embodiment, ammonium nitrate and a 15% ammophos solution used for the first embodiment are used. The nitrogen-phosphorus solution is prepared by adding a 15% ammophos solution and distilled water to the ammonium nitrate in a ratio of 1.88 kg: 1 l: 1 l. After the ammonium nitrate has completely dissolved, the solution is filtered.

[0059] To prepare a copper-cobalt solution according to the second embodiment, copper sulfate and cobalt nitrate used for the first embodiment are used by diluting them in distilled water in a ratio of 0.1 g: 0.1 g: 1 l.

[0060] Ammonium nitrate is used, for example, in the form of granules with a nitrogen content of N - 34%.

[0061] To prepare a 15% ammophos solution, ammophos with a phosphorus content of 48-50% is used. The ammophos solution is prepared in any known way. In one embodiment, to prepare a 15% ammophos solution, for example, 1.5 kg of ammophos is added to a ten-liter glass bottle. Tap water is poured into the bottle in the amount of 6-7 liters and mixed thoroughly, after which the resulting solution is infused for one day. After the specified period, the solution is thoroughly mixed and allowed to settle for another day. On the third day, after thorough mixing, when the ammophos granules are completely dissolved, the volume of the solution is brought to ten liters with tap water and the solution is allowed to infuse for another day. After settling, the transparent upper part of the solution is drained, leaving sediment at the bottom of the bottle. With this option for preparing the solution, its yield is 8-9 liters.

[0062] The preparation of a 1% solution of ferric chloride is carried out by any known method. In one embodiment, to prepare a 1% solution of ferric chloride, for example, one gram of ferric chloride is introduced into a 100 ml measuring flask and 100 ml of distilled water is added. The ferric chloride solution is prepared using distilled water. One gram of table salt (NaCI) is added to the prepared solution and shaken thoroughly until the salt is completely dissolved.

[0063] The preparation of a 0.1% solution of cobalt nitrate is carried out by any known method. In one embodiment, to prepare a 0.1% solution of cobalt nitrate, a sample of cobalt nitrate in an amount of 0.1 grams is dissolved in one liter of distilled water.

[0064] The preparation of a 0.01% solution of copper sulfate is carried out by any known method. In one embodiment, to prepare a 0.01% solution of copper sulfate, a sample of copper sulfate in the amount of 0.01 grams is dissolved in one liter of distilled water.

[0065] Reagent solutions are stored only in plastic containers. Storage of solutions in glass, ceramic or other containers is not allowed. It is not recommended to store reagent solutions in open light and at temperatures above room temperature.

[0066] Next, a carbon dioxide solution is prepared, for which naked oats are used as raw material. In one embodiment, wheat grains are used instead of naked oats to obtain a nutrient solution.

[0067] Fill the container for preparing the carbon dioxide solution one third full with naked oats. The raw material should not contain foreign impurities in the form of weed residues, foreign grain crops and other mechanical additives, as well as bird and rodent droppings, and should not be pickled or steamed. Mechanically processed cereals in the form of flattening, etc. are not allowed. Shrinking, split and fungal grains (dark coating) are not recommended for use. Oats that have been soaked in the rain and subsequently dried in drying units are not recommended for use.

[0068] A quality control check of oats is carried out by soaking 100-200 grams of naked oats in tap water. All grains soaked in water should lie on the bottom after thorough mixing. If some grains float to the surface, they are removed and the remaining amount is used to prepare a carbon dioxide solution.

[0069] Next, naked oats are poured into a container, preferably glass, to 1 / 3 of the volume of the said container and filled with the reagent solution prepared in the second stage. The reagent solution for preparing the nutrient medium is taken in such a volume that the reagent solution protrudes above the oats by a volume equal to the volume of oats. In this form, the container with the nutrient medium solution is kept for 24 hours at room temperature. After 24 hours, the acidity of the solution (pH) is measured, which should be from 3 to 5. If the acidity index is more than 5, the nutrient medium solution is kept for another 24 hours.

[0070] The amount of nutrient medium in the container with oats is maintained at the initial level by adding the reagent solution to the container with oats in accordance with the amount of nutrient medium consumed to obtain the chlorella suspension. The nutrient medium is consumed to obtain the chlorella suspension as needed, while in order to maintain the quality of the nutrient medium, it is drained from the container to the level of oats in the container, so that the oats should always remain in the solution. Replenishment of fresh reagent solution in the nutrient medium is carried out in such a way that the nutrient medium protrudes above the oats by a volume equal to the volume of oats. Mechanical impact on oats that are in use is not allowed. The container with the nutrient medium solution should not be under direct sunlight. The optimal temperature in the container with the nutrient medium solution should be 28-30 ° C.At night, the temperature of the solution may be reduced by 5 degrees below the specified norm.

[0071] At the third stage, after obtaining the nutrient medium, chlorella is cultivated in a device for obtaining a chlorella suspension.

[0072] The device for obtaining a chlorella suspension contains at least one vertical glass container 1, at one of the side walls of which a lamp 2 is mounted. Fig. 1, 2 shows an embodiment of the device with two containers 1, placed one next to the other with a gap in which the lamp 2 is placed. The shape of the containers 1 can be made in the form of a rectangular parallelepiped, a polygonal rectangular prism or arcuate in plan, wherein the walls of the arcuate containers facing each other are made convex from the lamp 2. In one of the embodiments, the container 1 can be made annular in plan, wherein the lamp 2 is placed in the center of the container 1.

[0073] The tanks 1 are mounted on a flat support surface 3 at the same level. The lamp 2 is also mounted on the said support surface 3 or is suspended from above in such a way that the light center of the lamp is located in the center of the thickness of the nutrient medium. For example, DRL or DNaT lamps are used as a light source in the lamp 2. The number of light sources in the lamp 2 and their power are determined by the dimensions of the tank and the requirements for the amount of luminous flux necessary for cultivating the chlorella suspension.

[0074] The lamps are powered by positron alternating current from a positron current unit, in which the rectification of alternating currents occurs by converting electrons and positrons into each other. In one embodiment, the positron current unit is made in the form of an Avramenko plug with a ferrite-magnetic filter. In another embodiment, the positron current unit contains two circuits, each of which contains a diode bridge, and the operation of such a positron current unit is based on the fact that the potential difference between the positive potential and the neutral conductor, as well as the potential difference between the neutral conductor and the negative potential, supplied to each of the diode bridges are equal. In a single-phase system, two diodes are always open in the diode bridge, the other two are always closed. Alternating current is an electric current that changes in magnitude over time, and usually in direction in an electric circuit. That is, it is a sinusoid.In the positive direction, the semiconductor bridge of a single-phase system passes a positron current driven by the potential difference between plus and zero. When a negative component of alternating current is applied to the bridge, the same diodes open and the current polarity changes from negative to positive, i.e. the electron motion vector changes to the positron motion vector, with the electrons turning into positrons. The positrons are converted in a similar manner in the second diode bridge, assembled on vacuum diodes.

[0075] Switching on the light source of the lamp 2 through the positron current unit allows to obtain a light source with a photonic information-active spin-field personalized initiation of autocorrection of the functional state, designed to initiate autocorrection and balance in the aquatic environment of viruses, fungi, protozoa, bacteria, helminths, their toxins. In simple words, the light source allows to destroy and block pathogenic microflora, as well as accelerate and stimulate the development of chlorella cells at the energy, genetic, cellular, organ levels. The tanks are equipped with shut-off valves made in the form of taps 4, one of which is mounted on the bottom for complete drainage of the nutrient medium from tank 1, and the other is mounted on one of the side walls with a gap of at least 40 mm from the bottom of tank 1 for draining the chlorella suspension.

[0076] The device for obtaining a chlorella suspension is equipped with a controller 5 (see Fig. 3), to which a relay 6 is mounted, through which a lamp 2 is connected to the controller 5. Relay 6 ensures switching on / off of the lamp 2 on command from the controller 5. At least one temperature sensor 7 is connected to the controller 5, mounted on the wall or inside the container 1, designed with the possibility of monitoring the temperature of the chlorella suspension in the container 1 in order to prevent a decrease or increase in the temperature required for cultivation. An increase in the temperature in the container 1 can be caused by thermal energy emitted by the light source of the lamp 2 or an increase in the temperature in the room where the device is located. A decrease in the temperature in the container 2 can be caused by a decrease in the temperature in the room where the device is located.To regulate the temperature, a heating element 14 mounted under the container 2 on its bottom and a cooling element 15 designed to cool the side walls of the container 2, for example, in the form of a chiller, with a cooling liquid circulating in it, can be connected to the controller via relay 6, for which purpose the chiller is connected to a pump (not shown in the figures), which can also be connected via relay 6 to the controller 5. In one embodiment, the heating element 14 and the cooling element 15 can be designed as Peltier elements.

[0077] The device for obtaining the chlorella suspension is equipped with a photo sensor 8 connected to the controller 5 and mounted on the wall of the container 1 located on the opposite side from the lamp 2, with the ability to control the level of the luminous flux from the lamp 2, necessary to ensure the required optical density or transmittance coefficient of the luminous flux of the chlorella suspension, which should be within the range from 1.4 to 1.8 for the optical density or from 2% to 4% for the transmittance coefficient. Valves 9 for controlling the shut-off valves can also be connected to the controller 5. A level sensor 10 can be connected to the controller 5, mounted inside the container 1 for monitoring the level of the nutrient medium (chlorella suspension) in the container 1.The controller 5 is provided with an interface module 11, made in the form of a screen, an indication unit 12, made, for example, in the form of a light and / or sound signal for displaying and notifying about the operating mode of the device and control elements 13, made with the possibility of entering information (buttons, switches) into the controller 5, changing the operating modes of the device. The indication unit 12 and / or control elements 13 can be combined with the interface module 11 and the indication and display of the operating modes of the device can be displayed on the screen of the interface module 11, and the control elements 13 can be made in the form of touch buttons on the screen.

[0078] To prepare the chlorella suspension at the third stage, the mother culture of the chlorella suspension based on the planktonic strain Chlorella vulgaris, for example, Chlorella vulgaris C-111, is added to tank 1 of the device for obtaining the chlorella suspension with the nutrient medium. The mother culture of the chlorella suspension is added in an amount of 20% of the volume of tank 1. The remaining volume of tank 1 is filled with the nutrient medium. In other words, the mother culture of the chlorella suspension and the nutrient solution are added in a ratio of 1:4, filling tank 1. During the cultivation of the chlorella suspension, hydroplasma is added to tank 1 at a rate of 1-10 grams per 12 liters and a hermetically sealed capsule with metaplasma, for example, GaNS, is placed at a rate of 2-20 grams per 12 liters of the nutrient medium.

[0079] Hydroplasma is a mixture of water and plasma, which is formed when electric and magnetic fields, as well as photon radiation on water. It has unique properties, such as high electrical conductivity, the ability to change its structure under the influence of external factors and the ability to interact with living organisms. It affects microalgae by changing their structure and activity, stimulates their growth and division. It is a concentrate of living water for health, beauty and longevity, used as a means for prevention and health promotion. Increases immunity and improves the resistance of the whole organism, suppresses viral and bacterial infections, cleanses cell membranes, providing stress resistance, promoting recovery from various diseases. Awakens the memory of a healthy cell.

[0080] Metaplasma is a concentrate of water and metal compounds. It has a positive effect on the central nervous system. Neutralizes the effects of radicals, restores the muscular system, and improves brain performance. It is a source of additional energy, allowing you to increase strength during physical activity, increase endurance, performance, and brain activity. It increases bioenergetics, vitality, and stress resistance. It promotes the development of reserved human resources, which is expressed in increased energy, vitality, endurance, performance, mental and physical activity. It allows you to restore bioenergetics and human energy centers. It allows you to neutralize the oxidative effects of free radicals, and normalizes the immune system. It improves the antitoxic function of the liver and kidneys. It activates hematopoiesis, improves blood circulation, and improves the permeability of cell membranes.

[0081] The production of chlorella suspension is based on the photosynthesis of microalgae, which is carried out in a container 1, using a nutrient medium, artificial lighting and at a certain cultivation temperature.

[0082] Irradiation with the light source of lamp 2 during the cultivation of the chlorella suspension is carried out during 4 light cycles, each of which includes 16 hours of irradiation, mainly during the daytime with a pause between light cycles of 8 hours, mainly at night. The total cultivation time is 96 hours.

[0083] In one embodiment, irradiation with light during the cultivation of the chlorella suspension can be carried out using an express method, in which irradiation is also carried out using four light cycles, each of which includes 10 hours of irradiation with a pause of 2 hours between light cycles. The total time for cultivating chlorella using the express method is 48 hours.

[0084] The cultivation temperature is 28-30°C.

[0085] Every day, when switching on and off the lamp 2, the required amount of carbon dioxide solution prepared in the second stage is poured into the container 1. The readiness of the suspension is determined by the optical density or transmittance. The optical density should be in the range from 1.4 to 1.8 inclusive, respectively, the transmittance should be in the range from 2% to 4% inclusive.

[0086] The resulting chlorella suspension is ready for use. The chlorella suspension is reproduced in a nutrient solution prepared using reagents according to the first or second embodiments. The required amount of reagents for different volumes of nutrient medium for cultivating chlorella are given in Tables 1, 2. Table 1.

[0087] Table 2.

[0088] The advantage of the planktonic form of chlorella is its exceptional adaptability to aquaculture conditions. Due to the fact that the chlorella culture is not demanding of carbon dioxide, the saturation of the culture in the present invention is carried out biologically, and the planktonic properties of the strain, characterized by free floating and uniform distribution in the water column, make it possible to exclude mechanical mixing of the suspension.

[0089] To obtain the chlorella suspension in the present invention, a minimum amount of chemical reagents, energy resources are used, environmental pollution is completely prevented, and the resulting products are environmentally friendly. The production of chlorella suspension has no waste, since all manufactured products are used for consumption.

[0090] Metaplasma creates plasma fields that have a beneficial effect on the generation of chlorella and the formation of high concentration metabolites.

[0091] High biological activity of planktonic strains makes it possible to reduce dosages and terms of consumption of chlorella suspension by people. The aftereffect allows achieving high rates of health restoration and normalization of the body. The use of chlorella suspension in the human diet allows for weight normalization up to 40% and restores immunity up to 99% due to obtaining additional muscle mass, increasing overall resistance, reproductive capacity and stimulating human metabolic processes.

[0092] The resulting chlorella suspension is a unique product that does not require significant labor costs or special personnel training and allows for the production of chlorella suspension all year round.

[0093] The process of producing the chlorella suspension can be carried out continuously, in which part of the chlorella suspension volume (up to 25%) is drained daily from tank 1 through tap 4 mounted on the side wall of the tank, but for this, in contrast to the above-stated ratio of the mother culture of the chlorella suspension and the nutrient solution poured into tank 1 of the device for obtaining the chlorella suspension of 1:4, the mother culture of the chlorella suspension and the nutrient solution are poured into the tank in a ratio of 3:1, that is, 25% of the volume of tank 1 of the nutrient solution is added to 75% of the volume of tank 1 of the mother culture. The irradiation mode is set according to the above-stated light cycles - 16 hours of irradiation, 8 hours of pause or 10 hours of irradiation 2 hours of pause. After daily draining of the finished chlorella suspension, the same amount of nutrient medium is added to tank 1.

[0094] In 2023, the author of the invention prepared chlorella suspensions using the claimed method in the device for obtaining a chlorella suspension described in the invention. As a device for preparing a chlorella suspension, the embodiments of the device with two containers 1 with a volume of 120 liters each described in the invention were taken. A lamp 2 with two DNaT-250 light sources was mounted between the containers 1 placed on a supporting surface 3 (table). The light sources of the lamp 2 were powered from a positron current source.

[0095] Two devices for preparing the chlorella suspension were used. In the first device, the chlorella suspension was prepared with a total cultivation time of 96 hours during 4 light cycles, each of which included 16 hours of irradiation with an 8-hour pause between light cycles (Method 1).

[0096] In the second device, the chlorella suspension was prepared using an express method with a total cultivation time of 48 hours during 4 light cycles, each of which included 10 hours of irradiation with a pause of 2 hours between light cycles (Method 2).

[0097] To prepare the nutrient medium, the following reagents and carbon dioxide were used per 1000 ml of tap water: ammonium nitrate - 0.2 g, ammophos, 15% solution - 0.1 ml, iron chloride, 1% solution - 0.15 ml, cobalt nitrate, 0.01% solution - 0.5 ml, copper sulfate, 0.01% solution - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml.

[0098] To obtain a chlorella suspension, a stock culture of chlorella suspension based on the planktonic strain Chlorella vulgaris IFR No. C-111 was used.

[0099] 10-100 grams of hydroplasma and one hermetically sealed capsule with metaplasma in the amount of 20-200 grams were added to each of the containers 1.

[0100] The cultivation temperature is 29-30°C.

[0101] Irradiation with light from the light source of lamp 2 during cultivation of the chlorella suspension for 96 hours was carried out during 4 light cycles, each of which included 16 hours of irradiation with a pause between light cycles for eight hours with the addition of nutrient medium to container 1 before each switching on / off of lamp 2 according to the following scheme: day 1: nutrient solution 400 ml, irradiation 06:00-22:00, pause 22:00-06:00; day 2: nutrient solution 600 ml, irradiation 06:00-22:00, pause 22:00-06:00; day 3: nutrient solution 600 ml, irradiation 06:00-22:00, pause 22:00-06:00; Day 4: nutrient solution 600 ml, irradiation 06:00-22:00, pause 22:00-06:00. Draining the chlorella suspension.

[0102] Irradiation with light from the light source of lamp 2 during the cultivation of the chlorella suspension using the express method was carried out for 4 light cycles, each of which included 10 hours of irradiation with a pause between light cycles for 2 hours with the addition of nutrient medium to container 1 before each switching on of lamp 2 according to the following scheme: day 1: nutrient solution 400 ml, irradiation 08:00-18:00, pause 18:00-20:00, nutrient solution 600 ml, irradiation 20:00-06:00, pause 06:00-08:00; Day 2: nutrient solution 600 ml, irradiation 08:00-18:00, pause 18:00-20:00, nutrient solution 600 ml, irradiation 20:00-06:00, pause 06:00-08:00;

[0103] Draining the chlorella suspension. The prepared chlorella suspension in the first and second devices was an aqueous suspension with dark green particles.

[0104] After preparing the chlorella suspension in one and the other way, its chemical studies were carried out.

[0105] The objective of the chemical studies was to quantitatively determine the content of amino acids, polyunsaturated fatty acids, palmitic, oleic, palminoleic, stearic, myristic, pentadecanoic, a-linoleic acid (Omega-3), erucic acids, fiber, vitamins, minerals, as well as the qualitative determination of antioxidants, chlorophyll, carotenoids.

[0106] Research methods:

[0107] - high-performance liquid chromatography with mass-selective detector;

[0108] - high-performance liquid chromatography with a fluorimetric detector;

[0109] - thin layer chromatography;

[0110] - spectrophotometry;

[0111] - gas chromatography with mass-selective detector;

[0112] - inductively coupled plasma mass spectrometry.

[0113] As a result of chemical studies, the content of the following substances in the chlorella suspension was established (see Table 3).

[0114] Table 3. When determining the qualitative determination in the chlorella suspension prepared by methods 1 and 2, the following compounds were detected:

[0115] - rutin (antioxidant);

[0116] - chlorophyll A;

[0117] - chlorophyll B;

[0118] - lutein;

[0119] - zeaxanthin.

[0120] Rutin is a bioflavonoid (vitamin P). When it enters the body, it acts as an antiulcer, hypoazatemic, anti-inflammatory, allergic, antitumor, radioprotective, choleretic agent, and is also an angioprotector and microcirculation corrector. Rutin plays a special role in blood and lymph microcirculation. Thanks to it, capillaries retain elasticity and permeability for biological fluids.

[0121] Zeaxanthin, along with lutein, nourishes the human visual organs and protects them from premature aging.

[0122] Linoleic, linolenic, 7,10-hexadecadienoic and 7,10,13-hexadecadienoic acids are essential and are part of the vitamin F complex.

[0123] The prepared chlorella suspension has virtually no algae taste and has a barely perceptible odor.

[0124] The prepared suspension of live chlorella retained its biological activity for 4 weeks, then a sediment fell out, which was a slightly gelatinous jelly-like mass. The oxidation-reduction potential of the chlorella suspension prepared by the claimed method was up to -300 mV, which is destructive for pathogenic microflora. The oxidation-reduction potential of the chlorella suspension prepared by known methods was up to -100 mV. In addition, as already noted above, the suspension of live chlorella prepared by the claimed method retained its biological activity for 4 weeks, and the chlorella suspension prepared by known methods retained its biological activity for only 2 weeks.

[0125] The use of hydroplasma allowed to create unique conditions, stimulating reproduction. The applied metaplasma, creating plasma fields, favorably influenced the regeneration of the culture. The created conditions provided the most favorable environment with the absence of pathogenic factors, promoting the development, growth and reproduction of chlorella.

Claims

FORMULA 1. A method for producing a chlorella suspension, including cultivating chlorella on a nutrient medium consisting of mineral and organic components by photosynthesis, characterized in that the following reagents and carbon dioxide are used to prepare the nutrient medium per 1000 ml of tap water: ammonium nitrate - 0.2 g, ammophos, 15% solution - 0.1 ml, iron chloride, 1% solution - 0.15 ml, cobalt nitrate, 0.01% solution - 0.5 ml, copper sulfate, 0.01% solution - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml, while naked oats are used as raw material for the preparation of carbon dioxide, which is filled with a solution of reagents prepared for the nutrient medium, the cultivation of chlorella is carried out in a device for obtaining chlorella suspension, for which the mother culture of chlorella suspension and nutrient solution are added to the container,hydroplasma at the rate of 1-10 grams per 12 liters and a hermetically sealed capsule with metaplasma at the rate of 2-20 grams per 12 liters of nutrient medium, and irradiation with a light source is carried out during 4 light cycles, while in the normal mode, each of the light cycles includes 16 hours of irradiation with a pause between light cycles of 8 hours, and with the express method, each of the light cycles includes 10 hours of irradiation with a pause between light cycles of 2 hours.

2. The method according to paragraph 1, characterized in that the mother culture of the chlorella suspension and the nutrient solution are introduced into the container in a ratio of 1:

4.

3. A method for obtaining a chlorella suspension, including cultivating chlorella on a nutrient medium consisting of mineral and organic components by photosynthesis, characterized in that the following reagents and carbon dioxide are used for the preparation of the nutrient medium at the rate of 1000 ml of tap water: ammonium nitrate - 0.2 g, ammophos, 15% solution - 0.1 ml, iron chloride, 1% solution - 0.15 ml, cobalt nitrate, 0.01% solution - 0.5 ml, copper sulfate, 0.01% solution - 0.5 ml, carbon dioxide, solution (pH 3-5) - 5-10 ml, while for the preparation of carbon dioxide, naked oats are used as raw material, which is filled with a solution of reagents prepared for the nutrient medium, the cultivation of chlorella is carried out in a device for obtaining a chlorella suspension, for which the mother culture of the chlorella suspension and a nutrient solution, hydroplasm at the rate of 1-10 grams per 12 liters and a hermetically sealed capsule with metaplasm at the rate of 2-20 grams per 12 liters of nutrient medium are added to the container, and irradiation with a light source is carried out in light cycles, while in normal mode the light cycle consists of 16 hours of irradiation and 8 hours of pause, and with the express method the light cycle consists of 10 hours of irradiation and 2 hours of pause, while the draining of the finished chlorella suspension is carried out daily with the replacement of the drained volume with nutrient solution.

4. The method according to paragraph 2, characterized in that the mother culture of the chlorella suspension and the nutrient solution are introduced into the container in a ratio of 3:

1.

5. The method according to paragraph 1 and paragraph 3, characterized in that the nutrient medium is prepared in compliance with the order of addition of reagents and carbon dioxide.

6. The method according to paragraph 1 and paragraph 3, characterized in that the solution of reagents for preparing the nutrient medium is taken in a volume necessary to exceed the oat level in the container by two times.

7. The method according to paragraph 1 and paragraph 3, characterized in that the container with the reagent solution and oats is maintained at a temperature of 23-30°C until the acidity level reaches a pH of 3 to 5.

8. The method according to paragraph 1 and paragraph 3, characterized in that the cultivation of the chlorella suspension is carried out at a temperature of 28-30°C.

9. A device for obtaining a chlorella suspension comprises at least one vertical glass container equipped with shut-off valves, a lamp with at least one light source powered by a positron alternating current and facing the side wall of the container, a controller configured to control the on / off switching of the lamp, a sensor for monitoring the temperature of the chlorella suspension in the container, heating and cooling elements configured to maintain a temperature of the nutrient medium in the container set by the controller, a photosensor configured to monitor the level of luminous flux from the lamp passing through the container, a sensor level control inside the tank, interface module, indicator unit and device controls.

10. The device according to item 9, characterized in that the container is made in the form of a rectangular parallelepiped, a polygonal rectangular prism, or an arc-shaped one in plan, wherein the wall of the arc-shaped container facing the lamp is made convex away from the lamp.

11. The device according to item 9, characterized in that the container is made annular in plan, and the lamp is mounted in the center of the container.

12. The device according to clause 9, characterized in that the light center of the luminaire is located at the center of the nutrient medium. Specifically, the luminaire uses a DRL or DNaT lamp as its light source.

13. The device according to item 9, characterized in that the shut-off valve of the container is at the bottom for completely draining the nutrient medium from the container and on one of the side walls of the container with a gap from the bottom of the container for draining the chlorella suspension.

14. The device according to item 9, characterized in that the shut-off valves are equipped with valves controlled by a controller.

15. The device according to item 9, characterized in that the heating and cooling elements can be made in the form of Peltier elements.

16. The device according to claim 9, characterized in that the indication unit and / or control elements can be combined with an interface module made in the form of a touch screen for indicating and displaying operating modes on the screen of the interface module, while the control elements are made in the form of touch buttons displayed on the screen.

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