Apparatus for collecting mouse bronchoalveolar lavage fluid and use method

Abstract

Disclosed are an apparatus for collecting mouse bronchoalveolar lavage fluid and a use method. The present invention pertains to the technical field of devices for collecting mouse bronchoalveolar lavage fluid. The apparatus comprises a suction and aspiration mechanism, and the suction and aspiration mechanism is connected to a collection mechanism by means of a conveyance tube. The collection mechanism comprises a collection bottle, and a first through hole and a second through hole are formed in a bottle cap of the collection bottle. An air guide tube passes through the first through hole, and a liquid guide tube passes through the second through hole. A first valve is arranged on the air guide tube, and a second valve is arranged on the liquid guide tube. Both the air guide tube and the liquid guide tube are connected to the conveyance tube by means of a three-way joint. The present invention uses the described apparatus for collecting mouse bronchoalveolar lavage fluid and use method. The structure is simple. The arrangement of the collection bottle eliminates the need for multiple transfers during lavage fluid collection that lead to loss. Moreover, controlling the opening and closing of the first valve, the second valve, and a third valve enables suction and aspiration in one device, so that the overall operation is convenient, which facilitates lavage fluid collection.

Description

A device for collecting bronchoalveolar lavage fluid from mice and its method of use Technical Field

[0001] This invention relates to the field of bronchoalveolar lavage fluid collection equipment for mice, and in particular to a device and method for collecting bronchoalveolar lavage fluid from mice. Background Technology

[0002] Mouse bronchoalveolar lavage fluid (BALF) is mainly used to detect inflammatory factors, exfoliated epithelial cells, and various pathological reactions in the lavage fluid under pathophysiological conditions (especially lung diseases) in mice, and it has diagnostic value for tumors and inflammation. In performing bronchoalveolar lavage experiments, the mouse trachea needs to be exposed and cut open, and sterile saline or PBS is injected into the trachea through an endotracheal tube. The bronchoalveolar lavage fluid is obtained through repeated aspiration.

[0003] In existing technologies, irrigation fluid is collected by aspirating with a syringe. However, due to the limited capacity of the collection device, it requires multiple replacements, making the operation complex and impractical. Furthermore, the collection process involves multiple transfers of liquid, resulting in significant losses and hindering the collection of irrigation fluid. Summary of the Invention

[0004] The purpose of this invention is to provide a device and method for collecting bronchoalveolar lavage fluid from mice. The device has a simple structure and a collection bottle, which eliminates the need for multiple transfers during the collection process, thus preventing losses. Furthermore, by controlling the opening and closing of the first, second, and third valves, suction and aspiration can be achieved in one device, making the overall operation convenient and facilitating the collection of lavage fluid.

[0005] To achieve the above objectives, the present invention provides a device for collecting bronchoalveolar lavage fluid from mice, comprising a suction mechanism connected to a collection mechanism via a delivery tube. The collection mechanism includes a collection bottle, the bottle cap of which has a first through hole and a second through hole. An air guide tube passes through the first through hole, and a liquid guide tube passes through the second through hole. The air guide tube has a first valve, and the liquid guide tube has a second valve. Both the air guide tube and the liquid guide tube are connected to the delivery tube via a tee.

[0006] Preferably, the aspiration mechanism includes a syringe, the injection head of which is connected to the delivery tube via a connecting plug, and the other end of the delivery tube is connected to a flexible tube.

[0007] Preferably, one end of both the gas guide tube and the liquid guide tube is provided with a thread, and the first through hole and the second through hole are provided with corresponding threads. The first through hole and the gas guide tube, and the second through hole and the liquid guide tube are all connected by threads.

[0008] Preferably, a first drain pipe is provided above the first valve, and a second drain pipe is provided above the second valve, with caps provided on both the first drain pipe and the second drain pipe.

[0009] Preferably, the collection bottle has graduations, a filter screen is provided inside the collection bottle, the filter screen is threadedly connected to the collection bottle, and a float is placed at the bottom of the collection bottle.

[0010] Preferably, the collection bottle is placed on a support assembly, the support assembly includes a support horizontal plate, a support vertical plate is fixed on the support horizontal plate, a clamp is fixed on the support vertical plate, and the collection bottle is placed inside the clamp.

[0011] Preferably, a third valve is provided on the delivery pipe between the gas guide pipe and the liquid guide pipe at one end near the liquid guide pipe.

[0012] Preferably, one end of the hose is provided at an angle.

[0013] The invention provides a method of using a device for collecting bronchoalveolar lavage fluid from mice, comprising the following steps:

[0014] Step 1: First, prepare the mouse by inserting a tubing into its trachea, closing the first and second valves, opening the third valve, and injecting the solution from the syringe into the mouse's trachea for irrigation. Then, open the caps on the first and second drain tubes to collect the remaining solution and replace the caps after collection.

[0015] Step 2: After flushing is completed, open the first and second valves and close the third valve. Pull the syringe handle outward. When you pull it outward, a negative pressure is generated in the collection bottle, which will cause the flushing fluid to flow through the delivery tube into the guide tube and finally into the collection bottle.

[0016] Step 3: After the rinsing fluid flows into the collection bottle, it is first filtered through a filter screen and then flows downward. The amount of rinsing fluid collected is observed by the height of the float.

[0017] Therefore, the present invention adopts the above-mentioned device and method for collecting bronchoalveolar lavage fluid in mice. It has a simple structure. By setting up a collection bottle, the lavage fluid does not need to be transferred multiple times during the collection process, thus avoiding losses. Furthermore, by controlling the opening and closing of the first valve, the second valve, and the third valve, the suction and aspiration can be achieved in one set of equipment. The overall operation is convenient and facilitates the collection of lavage fluid.

[0018] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0019] Figure 1 is a front view of an embodiment of the device and method for collecting bronchoalveolar lavage fluid in mice according to the present invention;

[0020] Figure 2 is an enlarged view of embodiment A of the device and method for collecting bronchoalveolar lavage fluid in mice according to the present invention.

[0021] Figure Labels

[0022] 1. Syringe; 2. Connecting bolt; 3. Injection head; 4. Tube; 5. Delivery tube; 6. First valve; 7. Second valve; 8. Third valve; 9. First discharge tube; 10. Second discharge tube; 11. Collection bottle; 12. Support plate; 13. Support plate; 14. Clamp; 15. Filter screen; 16. Float; 17. Bottle cap; 18. Cap; 19. Gas delivery tube; 20. Liquid delivery tube. Detailed Implementation

[0023] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning understood by one of ordinary skill in the art to which this invention pertains. The terms "first," "second," and similar terms used in this invention do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0025] Example 1

[0026] As shown in Figures 1 and 2, the present invention provides a device for collecting bronchoalveolar lavage fluid from mice, including a suction mechanism for injecting a solution into the trachea of ​​a mouse and then extracting the lavage fluid. The suction mechanism is connected to a collection mechanism via a delivery tube 5, which collects the lavage fluid without requiring further transfer via a syringe 1, thus reducing sample waste.

[0027] The suction mechanism includes a syringe 1. The injection head 3 of the syringe 1 is connected to the delivery tube 5 via a connecting plug 2. The other end of the delivery tube 5 is connected to a flexible tube 4. One end of the flexible tube 4 is set at an angle to prevent the flexible tube 4 from affecting the mouse's trachea.

[0028] The collection mechanism includes a collection bottle 11 for collecting the irrigation fluid. The collection bottle 11 is placed on a support assembly, which supports the collection bottle 11 and prevents it from tipping over and overflowing the irrigation fluid when the syringe 1 is moved. The support assembly includes a horizontal support plate 12, a vertical support plate 13 fixed to the horizontal support plate 12, and a clamp 14 fixed to the vertical support plate 13. The collection bottle 11 is placed inside the clamp 14, which secures the collection bottle 11 to the support assembly. The collection bottle 11 has graduations and a filter screen 15 inside. The filter screen 15 filters impurities in the irrigation fluid and removes foam generated by the irrigation fluid, preventing it from affecting the readings of the collection bottle 11.

[0029] The filter screen 15 is threadedly connected to the collection bottle 11, facilitating the replacement of the filter screen 15. A float 16 is placed at the bottom of the collection bottle 11. After the flushing fluid enters the collection bottle 11, the float 16 will rise as the liquid increases. When taking a reading, you only need to observe which scale line the float 16 is on to accurately read the data. If there is still foam flowing into the collection bottle 11 from the filter screen 15, the float 16 will prevent the reading from being affected by the foam.

[0030] The cap 17 of the collection bottle 11 is provided with a first through hole and a second through hole. The gas guide tube 19 passes through the first through hole and the liquid guide tube 20 passes through the second through hole. One end of the gas guide tube 19 and the liquid guide tube 20 are both provided with threads. The first through hole and the second through hole are provided with corresponding threads. The first through hole and the gas guide tube 19 and the second through hole and the liquid guide tube 20 are all connected by threads, which facilitates the replacement of the gas guide tube 19 and the liquid guide tube 20.

[0031] The suction mechanism extracts gas from the collection bottle 11 through the air guide tube 19, creating a negative pressure in the collection bottle 11. This negative pressure forces the rinsing fluid to flow into the collection bottle 11 through the liquid guide tube 20. The air guide tube 19 is equipped with a first valve 6, and the liquid guide tube 20 is equipped with a second valve 7. Both the air guide tube 19 and the liquid guide tube 20 are connected to the delivery pipe 5 via a tee. The first valve 6 controls whether the air guide tube 19 is open, and the second valve 7 controls whether the liquid guide tube 20 is open.

[0032] A first drain pipe 9 is located above the first valve 6, and a second drain pipe 10 is located above the second valve 7. Both the first drain pipe 9 and the second drain pipe 10 are equipped with caps 18. The first drain pipe 9 is used to recover the liquid remaining in the vent pipe 19, and the second drain pipe 10 is used to collect the liquid remaining in the liquid guide pipe 20. A third valve 8 is located on the delivery pipe 5 between the vent pipe 19 and the liquid guide pipe 20, near the end of the liquid guide pipe 20. The third valve 8 is used to control whether the delivery pipe 5 is open.

[0033] The invention provides a method of using a device for collecting bronchoalveolar lavage fluid from mice, comprising the following steps:

[0034] Step 1: First, prepare the mouse by inserting the tubing 4 into the mouse's trachea, closing the first valve 6 and the second valve 7, opening the third valve 8, injecting the solution in the syringe 1 into the mouse's trachea for irrigation, and opening the caps 18 on the first drain tube 9 and the second drain tube 10 to collect the remaining solution. After collection, put the caps 18 back on.

[0035] Step 2: After rinsing is completed, open the first valve 6 and the second valve 7, and close the third valve 8. Pull the handle of the syringe 1 outward. When pulling outward, a negative pressure is generated in the collection bottle 11, which will cause the rinsing fluid to flow through the delivery tube 5 to the guide tube 20 and finally into the collection bottle 11.

[0036] Step 3: After the rinsing fluid flows into the collection bottle 11, it is first filtered through the filter screen 15 and then flows downward. The amount of rinsing fluid collected is observed by the height of the float 16.

[0037] Therefore, the present invention adopts the above-mentioned device and method for collecting bronchoalveolar lavage fluid in mice. It has a simple structure. By setting up a collection bottle, the lavage fluid does not need to be transferred multiple times during the collection process, thus avoiding losses. Furthermore, by controlling the opening and closing of the first valve, the second valve, and the third valve, the suction and aspiration can be achieved in one set of equipment. The overall operation is convenient and facilitates the collection of lavage fluid.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A device for collecting bronchoalveolar lavage fluid from mice, characterized in that: The device includes a suction mechanism connected to a collection mechanism via a delivery pipe. The collection mechanism includes a collection bottle with a first through hole and a second through hole on its cap. A gas guide pipe passes through the first through hole, and a liquid guide pipe passes through the second through hole. A first valve is provided on the gas guide pipe, and a second valve is provided on the liquid guide pipe. Both the gas guide pipe and the liquid guide pipe are connected to the delivery pipe via a tee.

2. The device for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: The suction mechanism includes a syringe, the injection head of which is connected to the delivery tube via a connecting plug, and the other end of the delivery tube is connected to a flexible tube.

3. The device for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: One end of both the gas guide tube and the liquid guide tube is provided with a thread, and the first through hole and the second through hole are provided with corresponding threads. The first through hole and the gas guide tube, and the second through hole and the liquid guide tube are all connected by threads.

4. The device for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: A first drain pipe is provided above the first valve, and a second drain pipe is provided above the second valve. Both the first drain pipe and the second drain pipe are provided with caps.

5. The device for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: The collection bottle is marked with graduations, and a filter screen is installed inside the collection bottle. The filter screen is threadedly connected to the collection bottle, and a float is placed at the bottom of the collection bottle.

6. The apparatus for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: The collection bottle is placed on a support assembly, which includes a horizontal support plate, a vertical support plate fixed on the horizontal support plate, and a clamp fixed on the vertical support plate. The collection bottle is placed inside the clamp.

7. The device for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: A third valve is provided on the delivery pipe between the gas guide pipe and the liquid guide pipe, near the end of the liquid guide pipe.

8. The apparatus for collecting bronchoalveolar lavage fluid from mice according to claim 1, characterized in that: One end of the hose is angled.

9. A method of using the apparatus for collecting bronchoalveolar lavage fluid from mice as described in any one of claims 1-8, characterized in that: Includes the following steps: Step 1: First, prepare the mouse by inserting a tubing into its trachea, closing the first and second valves, opening the third valve, and injecting the solution from the syringe into the mouse's trachea for irrigation. Then, open the caps on the first and second drain tubes to collect the remaining solution and replace the caps after collection. Step 2: After flushing is completed, open the first and second valves and close the third valve. Pull the syringe handle outward. When you pull it outward, a negative pressure is generated in the collection bottle, which will cause the flushing fluid to flow through the delivery tube into the guide tube and finally into the collection bottle. Step 3: After the rinsing fluid flows into the collection bottle, it is first filtered through a filter screen and then flows downward. The amount of rinsing fluid collected is observed by the height of the float.

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